3-Methyladenine (3-MA)

Catalog No.S2767 Synonyms: NSC 66389

3-Methyladenine (3-MA) Chemical Structure

Molecular Weight(MW): 149.15

3-Methyladenine (3-MA) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation.

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4 Customer Reviews

  • MDC-labeled vacuoles were induced by AZD2014 and inhibited by autophagy inhibitor (3-MA). SMMC-7721 cells were treated with AZD2014 or rapamycin at concentrations of 100 and 600 nM, respectively, for 48 hours in the presence or absence of 3-MA, and then stained with MDC. Cells were immediately observed under a confocal microscope. Cells in the control group were treated with DMSO. bars, 20 μm.

    Am J Cancer Res, 2015, 5(1): 125-139. 3-Methyladenine (3-MA) purchased from Selleck.

    Cells were treated with DMSO, TDCIPP (100 μM) only, or pretreated with 3-MA before treatment with TDCIPP (100 μM). LC3 conversion and beclin-1 and p62 expression were assessed by western blotting at 24 h

    Food Chem Toxicol, 2017, 100:183-196. 3-Methyladenine (3-MA) purchased from Selleck.

  • PC-9/ER cells were treated with CX-4945 (5 mM) for 48 h in the presence or absence of 3-MA (2 mM) and Atg7 siRNA (100 nM). Cleavage of PARP-1 and caspase-3 was shown by Western blot analysis. CT: without CX-4945; CX: CX-4945; CX+G: CX-4945 + gefitinib; CX+E: CX-4945 + erlotinib.

    PLoS One, 2014, 9(12): e114000 . 3-Methyladenine (3-MA) purchased from Selleck.

    A: The survival ratio of AML12 cells was measured after treatment with rapamycin or 3-MA; B: Western blot was used to detect the expression of LC3 and P62 in AML12 cells treated with Rapamycin or 3-MA.

    World J Gastroenterol, 2016, 22(18): 4501-4514.. 3-Methyladenine (3-MA) purchased from Selleck.

Purity & Quality Control

Choose Selective PI3K Inhibitors

Biological Activity

Description 3-Methyladenine (3-MA) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation.
Targets
Vps34 [1]
(HeLa cells)
PI3Kγ [1]
(HeLa cells)
25 μM 60 μM
In vitro

The slight preference for Vps34 prevention by 3-Methyladenine probably arises from a hydrophobic ring specific to Vps34, which encircles the 3-methyl group of 3-Methyladenine. [1] 3-Methyladenine has been reported to cause cancer cell death under both normal and starvation conditions. 3-Methyladenine could also suppress cell migration and invasion independently of its ability to inhibit autophagy, implying that 3-Methyladenine possesses functions other than autophagy suppression. 3-Methyladenine elicits caspase-dependent cell death that is independent of autophagy inhibition. Treatment with 5 mM 3-Methyladenine reduces the percentage of glucose-starved HeLa cells displaying GFP-LC3 puncta to 23%. The levels of LC3-I are increasing and the levels of LC3-II are decreasing between 12 and 48 hours in cells that are treated with 3-Methyladenine. Conversion of LC3-I to LC3-II is suppressed by 3-Methyladenine. Treatment of HeLa cells with 3-Methyladenine at 2.5 mM or 5 mM for one day does not affect cell viability, whereas treatment with 10 mM 3-Methyladenine for one day causes a 25.0% decrease in cell viability. Treatment of cells with 2.5, 5 or 10 mM 3-Methyladenine for two days causes 11.5%, 38.0% and 79.4% decrease in viability, respectively. 3-Methyladenine decreases cell viability in a time- and dose-dependent manner. 3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest. [2] Suppression of autophagy by 3-Methyladenine inhibits SU11274-induced cell death. [3] Prolonged treatment with 3-Methyladenine (up to 9 hours) induces significant LC3 I to II conversion in wild type MEFs. Prolonged treatment with 3-Methyladenine, but not wortmannin, markedly increases GFP-LC3 punctuation/aggregation. 3-Methyladenine-induced LC3 conversion and free GFP liberation are ATG7-dependent. 3-Methyladenine treatment leads to evident increase of p62 protein level. 3-Methyladenine increases the p62 level even in Atg5−/− MEFs as well as in cells with DOX-mediated deletion of ATG5. 3-Methyladenine inhibits class I and class III PI3K in different temporal patterns. 3-Methyladenine-induced LC3 I to LC3 II conversion is dramatically compromised in Tsc2−/− cells compared with wild type cells.3-Methyladenine disrupts the anti-autophagic function of mTOR complex 1. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
95D NWrMPZB5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmrFN41O M4\Z[FJp NFzCbXFFVVOR NIHoelVz\WS3Y3XzJIdzd3e2aDDpcohq[mm2b4L5JIVn\mWldDDv[kBDTE2F NXruUXhtOjV5MU[1OlE>
A549  MkfGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXKzcW0> NYXWToxnOmh? MXfEUXNQ NWL2RYRHemWmdXPld{Boem:5dHigbY5pcWKrdH;yfUBm\m[nY4Sgc4YhSkSPQx?= NHPYdGwzPTdzNkW2NS=>
95D NWS0dol7SXCxcITvd4l{KEG|c3H5 MW[1cW0> M4jUfFJp NH3ucHhFVVOR NYPMdFh5cW6qaXLpeJMhSkSPQz3pcoR2[2WmIHHwc5B1d3SrYzDj[YxtKGSnYYTo M1rhflI2PzF4NU[x
A549  NF6yXG1CeG:ydH;zbZMhSXO|YYm= MmO3OY1O MoHlNog> NEPONXNFVVOR MWHpcohq[mm2czDCSG1ENWmwZIXj[YQh[XCxcITveIlkKGOnbHyg[IVifGh? M2L2e|I2PzF4NU[x
NBL-W-S  MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkfBNY1O MoH0Oog> MWHlcohidmOnczDHRW5VNTZzIITvfIlkcXS7 M{nwNFI2OzJ|MkKy
NBL-W-S  MlzPRZBweHSxc3nzJGF{e2G7 MUSxcW0> Ml3POog> Mmn6bY5kemWjc3XzJINmdGxiYYDvdJRwe2m|IHnu[JVk\WRiYomgS2FPXC14MR?= NVL2UVF7OjV|MkOyNlI>
A2780cp  NG\QdXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWOxcW0> NXXpd4hpOWh? NVrFNIw1cW6lcnXhd4V{KGOrc4DsZZRqdi2rbnT1Z4VlKGOnbHyg[IVifGh? MnXNNlU{OjJ4OUS=
A549  NVzwVm8{TnWwY4Tpc44hSXO|YYm= M{TXXlVuVQ>? NHS3OFEzcA>? NXPaTIhRe3WycILld5NmeyCVUFOgbY5lfWOnZDDhZ4N2dXWuYYTpc44hd2ZiTFOzMWlK NIC5[JEzPTJ6NU[yPC=>
H157 Ml64SpVv[3Srb36gRZN{[Xl? MnTJOY1O MnT3Nog> NIjjU2V{fXCycnXzd4V{KFOSQzDpcoR2[2WmIHHjZ5VufWyjdHnvckBw\iCOQ{OtTWk> M1v1cFI2Ojh3NkK4
MDR NYrNRYJWSXCxcITvd4l{KEG|c3H5 MkHSNVBuVQ>? MWS2bC=> NH:xN49{fHKnbnf0bIVveyC2aHWgdI94\XJib3[gZY51cWOjbnPldkBlenWpcx?= MWGyOVAyQTdyMR?=
HT-29  NFPJOZlCeG:ydH;zbZMhSXO|YYm= NVjnfGFKOW2P MXi0PIg> M3nzRWROW09? NEnoeJVmdmijbnPld{Bjd3K2ZYrvcYljNWmwZIXj[YQh[2WubDD2bYFjcWyrdImgcI9{ew>? MmfGNlQ5PDJzNUi=
Microglia  MWnBdI9xfG:|aYOgRZN{[Xl? NXX2WFRoPW2P NUDuVVViOjSq M1z5UoRm[3KnYYPld{BpgXCxeHnhMYlv\HWlZXSgZ4VtdCCmZXH0bC=> NULyOWJQOjR6MUi2NFE>
A2780cp MWPBdI9xfG:|aYOgRZN{[Xl? MX6yMlVuVQ>? MX:xbC=> MVfk[GgzVw>? NXPm[pRK\W6qYX7j[ZMh[2m|cHzheIlvNWmwZIXj[YQh[2WubDDk[YF1cA>? MmLnNlQ5OTd7NE[=
HepG2 MXLGeY5kfGmxbjDBd5NigQ>? MkTXOY1O M120[FRp NE\SU5lqdmO{ZXHz[ZMhUExicnXs[YF{\Q>? NWf4WJlwOjR5MUO1PFc>
A549 MUXBdI9xfG:|aYOgRZN{[Xl? NEHaRVQ2dU1? M3PTclQ5cA>? NVHue2pY\GWlcnXhd4V{KHSqZTDw[ZJk\W62YXflJI9nKGOnbHyg[IVifGhiYX7kJIV5eHKnc4Ppc44hdGW4ZXzzJI9nKGOjc4Dhd4UuOyxiQnXjcIlvNTFiYX7kJGxEOy2LSR?= NVHqeJpoOjR5ME[zNFM>
U2OS NXezNoQ2SXCxcITvd4l{KEG|c3H5 M1T1W|ExdU1? NXrtcmtLOjSq MV3pcpRmdnOrZnnld{B1cGViZ4Lve5RpKGmwaHnibZRqd25ib3[geIhmKFV{T2OgZ4VtdHNiaX7keYNm\CCkeTDEc5g> MmmxNlQ3OzlyMUO=
Saos-2 MUXBdI9xfG:|aYOgRZN{[Xl? NEfSVHYyOG2P M2rwNlI1cA>? M4rwUYlvfGWwc3nmbYV{KHSqZTDndo94fGhiaX7obYJqfGmxbjDv[kB1cGViVULPV{Bk\WyuczDpcoR2[2WmIHL5JGRwgA>? M4nCNlI1PjN7MEGz
A549 M2TocmFxd3C2b4Ppd{BCe3OjeR?= M1Ls[|ExdU1? MUS0PIg> M3OwNoFk[2WuZYLheIV{KHSqZTDy[YR2[3Srb36gc4Yh[2WubDD2bYFjcWyrdImgbY5lfWOnZDDifUBRXFh? M3v6blI1PjJ4N{Ky
HCT116  MXTBdI9xfG:|aYOgRZN{[Xl? MVO1cW0> NGrkZ5YzPGh? NE\PSFRmdmijbnPld{B1cGViYYDvdJRwe2m|IHnu[JVk\WRiYomgZZBq\2WwaX6= NHHUeGMzPDZ{NkWyNi=>
HGC-27 NXrSfldsTnWwY4Tpc44hSXO|YYm= MVqxNI1O Mki3NYg> NGHqeGpqdmirYnn0d{B1cGViY3XscEB3cWGkaXzpeJkhdG:|czDifUBTSURyMEGgc5IhVUtvMkKwOi=> NW\sXIpSOjR2MU[zOFk>
U2OS  MX\GeY5kfGmxbjDBd5NigQ>? NW\ZfGV5OC53L{HtUS=> MXK0PIg> NWPFSot2cW6mdXPld{B{[Wyrbn;tfYNqdi2rbnT1Z4VlKGOnbHygeoli[mmuaYT5JIxwe3N? NEXLWpQzPDN3OEO0Ni=>
MG-63 NH3qeppHfW6ldHnvckBCe3OjeR?= MkTRNE42NzGvTR?= MVO0PIg> M{\Bd4lv\HWlZYOgd4FtcW6xbYnjbY4ucW6mdXPl[EBk\WyuII\pZYJqdGm2eTDsc5N{ M{[wSVI1OzV6M{Sy
MG-63 MX;BdI9xfG:|aYOgRZN{[Xl? M4fJO|AvPS9zbV2= MontN|Jp NFHDeZhmdmijbnPld{B{[Wyrbn;tfYNqdi2rbnT1Z4VlKGOnbHygZZBweHSxc3nz MUWyOFM2QDN2Mh?=
MG-63 M4DxWmFxd3C2b4Ppd{BCe3OjeR?= NU\6OopvOTCvTR?= M{XKeFEzcA>? MWTlcohidmOnczDEVE1qdmS3Y3XkJIFxd3C2b4Ppdy=> MXyyOFM2QDNyMR?=
NTUB1 MV7BdI9xfG:|aYOgRZN{[Xl? M1nZ[VVuVQ>? NFrPbIEyNjWq MXzwc5RmdnSrYYTld{Bk\WynY3;4bYIucW6mdXPl[EBieG:ydH;zbZM> MVmyOFM1QTF5Nh?=
T24 NVTCd3lNSXCxcITvd4l{KEG|c3H5 M4fPcFVuVQ>? MWWxMlVp NY\XcYxXeG:2ZX70bYF1\XNiY3Xs[YNwgGmkLXnu[JVk\WRiYYDvdJRwe2m| MWGyOFM1QTF5Nh?=
SGC-7901  M{fkc2Fxd3C2b4Ppd{BCe3OjeR?= M1Ltb|JuVQ>? MoLCNYg> NEHFWlZqdmO{ZXHz[ZMhS0FvNDDpcoR2[2WmIHHwc5B1d3Orcx?= M3vUUFI1OzJzM{Sw
SMMC-7721 M1PpZmFxd3C2b4Ppd{BCe3OjeR?= MlXYNo1O M2\sdlFp NV7tWIlLcW6lcnXhd4V{KEODLUSgbY5lfWOnZDDhdI9xfG:|aYO= NFLOVZkzPDN{MUO0NC=>
ECSCs  MYnBdI9xfG:|aYOgRZN{[Xl? MlTwNVBuVQ>? NHvjTIM1cA>? NWjjSJEz\GWlcnXhd4V{KHKjcHHtfYNqdi22cnXheIVlKGGyb4D0c5Nqew>? M4jzU|I1OzF7MUC5
MCF-7  NYHRTJRzTnWwY4Tpc44hSXO|YYm= MX2xNI1O MljHNlRp MUTpcohq[mm2czD0bIUh[XW2b4DoZYd6KGmwZIXj[YQh[nliY3jlcY91cGW{YYD5JIRzfWe| M13pdVI1OzF3NUe4
UOK257 NFq3U|BCeG:ydH;zbZMhSXO|YYm= Mo\2OY1O M{fiWFNp NYrUcXZP\W6qYX7j[ZMheGGlbHn0ZZhmdC2vZXTpZZRm\CCjcH;weI9{cXQEoB?= M{LUXVI1OzB3NkC0
ACHN-5968 M33M[GFxd3C2b4Ppd{BCe3OjeR?= NVvkUVBFPW2P MXqzbC=> NV;0eFJo\W6qYX7j[ZMheGGlbHn0ZZhmdC2vZXTpZZRm\CCjcH;weI9{cXQEoB?= NVjEVno1OjR|MEW2NFQ>
Huh7 NUH5TVY4SXCxcITvd4l{KEG|c3H5 MWWycW0> Mkn1NVJp Ml;ySG1UVw>? NEnzXnpqdmirYnn0d{BCYkR6MEW1MYlv\HWlZXSgZ4VtdCCmZXH0bC=> MVmyOFI6PzNyMB?=
Hep3B M2XZeWFxd3C2b4Ppd{BCe3OjeR?= MlK3No1O M2jJcVEzcA>? M13vd2ROW09? MnPibY5pcWKrdIOgRXpFQDB3NT3pcoR2[2WmIHPlcIwh\GWjdHi= NHizdWEzPDJ7N{OwNC=>
RKO M3TMb2Z2dmO2aX;uJGF{e2G7 M4\kU|JuVQ>? NUnKWFl4OWh? NYDr[2ZyTE2VTx?= NFSxNJNmdmijbnPld{Bk\WyuIHTlZZRpKGK7IHflcIRidmGveXPpci=> NXHyU|hnOjR{OUG3O|c>
HepG2 E47 MWPGeY5kfGmxbjDBd5NigQ>? NHW0[5EzNjWvTR?= NIX1elU1QGh? NUSybVhYcW6lcnXhd4V{KHSqZTD0c5hq[2m2eTDv[kBCSSxiQmPPMEBidmRiQ1PsOC=> NYHaWXlUOjR{N{O3N|g>
LoVo  M{\kNWFxd3C2b4Ppd{BCe3OjeR?= M2LkW|VuVQ>? MVG0PIg> MmDK[Y5p[W6lZYOgSGNCNWmwZIXj[YQh[XCxcITvd4l{Ng>? NVTIZ25iOjR{MEG4NVI>
WiDr NFLyZpZHfW6ldHnvckBCe3OjeR?= MYqxNI1O MWWxbC=> M1O0OYlvcGmkaYTzJHBESkxvaX7keYNm\CCOQ{OgTWkh\XiycnXzd4lwdg>? M{nJO|I1OTlyNEi5
H1299  NGG0ZXlHfW6ldHnvckBCe3OjeR?= NF[5cpYyOG2P NGHkS5E1cA>? NG[3b|lqdmO{ZXHz[ZMh[2m|cHzheIlvNWmwZIXj[YQh[2WubDDk[YF1cA>? MlrpNlQyPzN{MEi=
H460 MVTGeY5kfGmxbjDBd5NigQ>? NV7De2xOOTCvTR?= M3\BU|Rp NW\NVYxJcW6lcnXhd4V{KGOrc4DsZZRqdi2rbnT1Z4VlKGOnbHyg[IVifGh? M4LRNlI1OTd|MkC4
A549 NV;DZ5FDTnWwY4Tpc44hSXO|YYm= Mki4NVBuVQ>? M4HvVFRp NF\QXJRqdmirYnn0d{BifXSxcHjh[5khcW6mdXPl[EBjgSCrcoLh[IlifGmxbh?= MUmyOFE1Ojd|NR?=
Hep3B NVnlZZN{SXCxcITvd4l{KEG|c3H5 NXzLbZBTPW2P MX6yOIg> MYfheJRmdnWjdHXzJHRPTi4QsTDwdo91\WO2aX;uJIFo[Wmwc4Sgd4VzfW1ic4ThdpZifGmxbj3t[YRq[XSnZDDhdI9xfG:|aYO= M3X5TlI1ODZ4Nkmz
SMMC-7721 NXf3TpY6SXCxcITvd4l{KEG|c3H5 NW\Wc|BwPW2P MUWyOIg> Ml;RZZR1\W63YYTld{BVVkZvzsGgdJJwfGWldHnvckBi\2GrboP0JJNmenWvIIP0ZZJ3[XSrb36tcYVlcWG2ZXSgZZBweHSxc3nz MofDNlQxPjZ4OUO=
HO8910 M1nld2Fxd3C2b4Ppd{BCe3OjeR?= MkX4NVBuVQ>? Mn\rNVJp Mn;W[Y5p[W6lZYOgRlE6NWmwZIXj[YQh[XCxcITvd4k> NUDoPWtEOjN7OEO2NVA>
MCF7 MXjGeY5kfGmxbjDBd5NigQ>? NYqzUnJ{PW2P M1TtWVI1cA>? Mnv6bY5kemWjc3XzJGN2VyCrbnT1Z4VlKGOnbHyg[IVifGh? NVnxPW1SOjN7NkK2Nlk>
HONE-1  NY\UXVd{TnWwY4Tpc44hSXO|YYm= NGfST4E2dU1? MnXwNYg> MWjy[ZBz\XO|ZYOgOpIudWWmaXH0[YQhWk:VIIDyc4R2[3Srb36= MkLmNlM5QTJ|NUi=
HeLa NXjWXlJNTnWwY4Tpc44hSXO|YYm= M{Xhe|ExdU1? M3jyVFJp NWfmWnF7e3WycILld5NmeyCOQ{OgTWkh\XiycnXzd4l{d25? M1;yVlI{QDZ2N{O4
HepG2 M3;KeWZ2dmO2aX;uJGF{e2G7 NUfpepZ3OTCvTR?= MViyOIg> MkfobY5pcWKrdIOgd4lVUUeDUj2gZY5lKEiEU2OtbY5lfWOnZDDheZRweGijZ4m= MV2yN|gyPzB2MB?=
SH-SY5Y NFvweFdHfW6ldHnvckBCe3OjeR?= NWfTV3lzOW2P NHfJXmgzPGh? MX7pcohq[mm2czD0bIUh[XW2b4DoZYd6KGmwZIXj[YQh[nliVF;DVC=> MVqyN|c1OzF2OB?=
SH-SY5Y NEm0WmpCeG:ydH;zbZMhSXO|YYm= MkjBOY1O MnX1NYg> MXLhZo9tcXOqZYOgZ4Vt[XO2cn;sJI5mfXKxcILveIVkfGm4ZTDl[oZm[3R? NYTpcoZ5OjN4MUmzPVU>
PC12  M1P1PWZ2dmO2aX;uJGF{e2G7 MlnXNVBuVQ>? MUGyOIg> NFr0c254[XSnch?= MkTtxsBqdmirYnn0d{BkcHmvb4TyfZB{cW5vbHnr[UBxem:2ZXHzc41idCCjY4Tpeol1gS5? MljhNlM3ODN7N{m=
OV2008 NF\sdoFCeG:ydH;zbZMhSXO|YYm= NVHMS45FPW2P M3PQWlI1cA>? NFTJS2Rkd264ZYL0d{BHXFl5MkCge4l1cCCFRFTQJIlvfG9iYX6gZYRlcXSrdnWg[YZn\WO2IITve4Fz\HNia3nscIlv\yCxdnHybYFvKGOjbnPldkBk\Wyucx?= MkfxNlM2QTJ{OEG=
A2780 Ml7ORZBweHSxc3nzJGF{e2G7 NYP6SHpQPW2P MXyyOIg> NYG5Noo6[2:wdnXyeJMhTlS\N{KwJJdqfGhiQ1TEVEBqdnSxIHHuJIFl\Gm2aY\lJIVn\mWldDD0c5diemS|IHvpcIxqdmdib4\hdolidiClYX7j[ZIh[2WubIO= MUKyN|U6OjJ6MR?=
Saos-2  Mn[zRZBweHSxc3nzJGF{e2G7 M13xNVFuVQ>? MVG5Oog> MnrPbY5kemWjc3XzJINmdGxiZHXheIghcW6mdXPl[EBjgSCSQ2i= M1fIWFI{PTZ|MUex
1321N1 MYjDfZRwfG:6aXPpeJkhSXO|YYm= NIqwSI42dU1? MnHGNlRp M{fVWZBzd3SnY4TzJINmdGxiYXfhbY5{fCCSQ16tbY5lfWOnZDD0c5hq[2m2eR?= MWSyN|UzPTJ4NR?=
SH-SY5Y MojhR5l1d3SxeHnjbZR6KEG|c3H5 MW[1cW0> Mmf0NlRp MonpbY5kemWjc3XzJHBEViC2b4jpZ4l1gQ>? NVn5V4VVOjN3MkWyOlU>
HT-29  NF30[FZHfW6ldHnvckBCe3OjeR?= NXrPflBVOW2P M2PDPVQ5Nzl4aB?= NViwRYtwcW6qaXLpeJMhSU2SSzDpcoR2[2W|IHH1eI9xcGGpaXOgZ4VtdCCmZXH0bC=> MkTKNlM2ODh{N{K=
OR6 MlrDSpVv[3Srb36gRZN{[Xl? NUG0R5gyOTCvTR?= MYG3Nog> NX:0W5R6e3WycILld5NmeyCKQ2[gdoVxdGmlYYTpc44h[W6mIH\vdo1ifGmxbjDv[kBifXSxcHjh[49{d22ncx?= NIrJc2IzOzN7NUi3OS=>
Hela  MX7GeY5kfGmxbjDBd5NigQ>? M3vud|VuVQ>? MVuyOIg> MVPpcohq[mm2czDzeIFzfmG2aX;uMYlv\HWlZXSgZZV1d3CqYXf5 NE\wdXIzOzN7NU[3PS=>
MCF-7  MnrDSpVv[3Srb36gRZN{[Xl? MnPTOY1O MlTiNlRp MmjDbY5pcWKrdIOgd5Rien[jdHnvck1qdmS3Y3XkJIF2fG:yaHHnfS=> M{PTNlI{Ozl3Nke5
HUVECs M1PyVWZ2dmO2aX;uJGF{e2G7 MnKwN41O MorINlRp MljTZoxw[2u|IITo[UBxem:2ZXP0bZZmKGWoZnXjeEBw\iC{ZYP2[ZJifHKxbDDifUBqdmirYnn0bY5oKGG3dH;wbIFogQ>? NUHjcohTOjN|NUi5Nlg>
T24 NXrobmttTnWwY4Tpc44hSXO|YYm= MljjNVBuVQ>? MVexbC=> MVXy[YR2[2W|IITo[UBkdGWjdnHn[UBw\iCOQ{OgZYZ1\XJiYnHpZ4FtcW5idILlZZRu\W62 MoHGNlM{PTRyOEC=
U251MG  NITHRYJHfW6ldHnvckBCe3OjeR?= MnXiN41O NVnjV5l2OWh? NHX6RXJ{fXCycnXzd4V{KEyFMz3JTUBxem:2ZXnuJIV5eHKnc4Ppc44> NGm3dFEzOzN|OE[xPC=>
GTL-16  NYXzPINnSXCxcITvd4l{KEG|c3H5 NX61OYppPW2P M3HtXVI1cA>? NUDtOYtwemWmdXPld{Bk\WyuII\pZYJqdGm2eTDhd{Bkd22yYYLl[EB1dyClZXzsd{B1emWjdHXkJJdqfGhiTVXUJIlvcGmkaYTvdpM> NXf6fXNMOjN|MUO0PVA>
T-47D NEK1OYlHfW6ldHnvckBCe3OjeR?= Ml3kNVBuVQ>? NEe3NpUzcA>? MXXpcohq[mm2czDheZRweGijZ4mgdJJw[2W|czDhcoQhcW6lcnXhd4V{KHKjcHHtfYNqdiCrbnT1Z4VlKGGyb4D0c5Nqew>? M4X5dFI{OzByMEK2
PaCa44 M2q5fGFxd3C2b4Ppd{BCe3OjeR?= NGrJS2ozNjWvTR?= NEezfoMycA>? NWLJeYZuemWmdXPld:Kh\2WwaYDpck1u\WSrYYTl[EBieG:ydH;zbZM> NITRe5IzOzF{NEGxNi=>
MDA-MB231 MVjGeY5kfGmxbjDBd5NigQ>? NXLVS3Z4PW2P MnTTNYg> MkPJbY5kemWjc3XzJJJme3[ncnH0do9tNW2nZHnheIVlKGOjc4Dhd4Uh[WO2aY\heIlwdiCjbnSgZ4VtdCCmZXH0bC=> M{HuZVI{ODh6OEWw
HeLa MWDBdI9xfG:|aYOgRZN{[Xl? M3zCd|ExdU1? M3\KUlJp NGfG[ZNl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgZ48ufHKnYYTt[Y51KHerdHigVGVK NFnLR2YzOzByMEGzOS=>
SK-HEP-1 M2fHdmFxd3C2b4Ppd{BCe3OjeR?= M{nmZ|ExdU1? MWGxbC=> NYLXN|Y5eHKxdHXjeJMh[WejaX7zeEBifXSxcHjh[5kh[W6mIHnu[JVk\XNiYYDvdJRwe2m|IHnuJIJ2\mGuaX6teJJm[XSnZDDj[Yxtew>? NYPYNGpKOjJ6NUi2OFk>
HepG2 NH3MOZZCeG:ydH;zbZMhSXO|YYm= NF:wfYQ{dU1? NXzzSGpoPWh? MVvy[YR2[2W|IHPlcIwh[XCxcITvd4l{KGmwZIXj[YQh[nliUVTz NVztPWxPOjJ6M{[1PVU>
MCF-7  MnHDSpVv[3Srb36gRZN{[Xl? M3Hse|JuVQ>? NGPqSIwzPGh? M4rYUolvcGmkaYTzJIF2fG:yaHHnfUBqdmS3Y3XkJIJ6KFSPwrC= NH;TVJkzPDl5ME[3Oi=>
MDA-MB-231 M1j2XGZ2dmO2aX;uJGF{e2G7 MXmycW0> NGjD[YwzPGh? NHqzW|ZqdmirYnn0d{BifXSxcHjh[5khcW6mdXPl[EBjgSCWTdMg M2DUT|I1QTdyNke2
MCF-7  M2jNRmZ2dmO2aX;uJGF{e2G7 M32yO|JuVQ>? NGPrcG01QGh? NIfSU4Zxem:vb4Tld{BVVS2rbnT1Z4VlKGOnbHyg[IVifGh? M1PmSFI1QTdyNke2
MDA-MB-231 MoHMSpVv[3Srb36gRZN{[Xl? M1u2TVJuVQ>? MXu0PIg> MYDwdo9ud3SnczDUUU1qdmS3Y3XkJINmdGxiZHXheIg> MUeyOFk4ODZ5Nh?=
PANC-1  NWrCXFE6SXCxcITvd4l{KEG|c3H5 NFnIU|EydU1? MV20PIg> M3r2cGROW09? NEjmdmVmdmijbnPld{Bjd3K2ZYrvcYljNWmwZIXj[YQh[2WubDD2bYFjcWyrdImgcI9{ew>? NXv3PFdtOjR6NEKxOVg>
MDA-MB 231 NHLmWWJCeG:ydH;zbZMhSXO|YYm= NWT2NYp3PW2P NELoSHkxNjWq NGjmXYxud2S3bHH0[ZMhXG:lb33pcuKvyqCrbnT1Z4VlKGGyb4D0c5Nqew>? MkT4NlQ5OzB5OEG=
Microglia  NYP4NFQ1SXCxcITvd4l{KEG|c3H5 MlrkOY1O Mn:yNlRp MoXK[IVkemWjc3XzJIh6eG:6aXGtbY5lfWOnZDDj[YxtKGSnYYTo MX6yOFgyQDZyMR?=
A2780cp NGOxW21CeG:ydH;zbZMhSXO|YYm= MmTPNk42dU1? MXSxbC=> MX3k[GgzVw>? NFT6WZhmdmijbnPld{BkcXOybHH0bY4ucW6mdXPl[EBk\WyuIHTlZZRp NVTBZVZ2OjR6MUe5OFY>
HepG2 M1XwOGZ2dmO2aX;uJGF{e2G7 NHT4ZZM2dU1? NWexeHpFPGh? MYPpcoNz\WG|ZYOgZ4VtdHWuYYKgcIV3\Wy|IH;mJGhNyqB? M4\CfVI1PzF|NUi3
U2OS MmfaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWWxNI1O NVjmNZlROjSq NWjORY43cW62ZX7zbYZq\XNidHjlJIdzd3e2aDDpcohq[mm2aX;uJIlv\HWlZXSgZpkhTG:6 MnzaNlQ3OzlyMUO=
HCT116 M1PuXmFxd3C2b4Ppd{BCe3OjeR?= MX21cW0> M{HSRVI1cA>? NWLub|dsTE2VTx?= MmPF[Y5p[W6lZYOgZZBq\2WwaX6tbY5lfWOnZDDj[YxtKGSnYYTo MV[yOFYzPjV{Mh?=
MCF-7  Mnz4RZBweHSxc3nzJGF{e2G7 NYnsZZplOC5zbV2= M2H0WlZp NYTUSmRn\W6qYX7j[ZMhe2m{dHnuc4wucW6mdXPl[EBieG:ydH;zbZM> Mk\FNlI4PTF7OEm=
PC-3  NULOW3JZSXCxcITvd4l{KEG|c3H5 Mor5No1O MXuybC=> M{m3NIlv[3KnYYPld{BQWklvaX7keYNm\CClZXzsJIRm[XSq M2K0VlIzPzR3NUiw
U251  NUXDT|R4SXCxcITvd4l{KEG|c3H5 NYKwNmJUPW2P Ml\QNlRp NYfMbo41cW6lcnXhd4V{KFNzLXnu[JVk\WRiY3XscEBl\WG2aB?= NHThfmgzOjV5OUe4PC=>
HeLa  MYfBdI9xfG:|aYOgRZN{[Xl? NH[2UXE2dU1? NHLXfnQzPGh? NYWzOG12cW6mdXPld{Bk[XOyYYPlMYRmeGWwZHXueEBk\WyuIHTlZZRp MVSyNlU1PTF{OB?=
A549 MWLGeY5kfGmxbjDBd5NigQ>? MYOwMlFuVQ>? NHzvUnczPGh? NWC2ZY1De3WycILld5NmeyCVVUGxNlc1NWmwZIXj[YQh[2WubDDk[YF1cA>? NX7kS4c3OjJ2Nk[5OlA>
pDCs Mn;3SpVv[3Srb36gRZN{[Xl? MYWxNI1O NXXnWJR[OC53aB?= NWLET3QxyqC{ZXT1Z4V{KHSqZTDpcoR2[3Srb36gc4YhUU[QLd8xJIJ6KHO|Ul7BOFA> MUKyNlM6PjV7OR?=
BGC-823 MX;GeY5kfGmxbjDBd5NigQ>? M17yZ|VuVQ>? NIPoSW0zcA>? M1HHcYlvcGmkaYTzJJRp\SC{YYTlJI9nKGG3dH;wbIFocWNiY3XscJM> NGLITpQzOjN{MkG1Ni=>
U937 MVzGeY5kfGmxbjDBd5NigQ>? NUXXXYV2Om2P NGfzXWMyOmh? M{TvPYRm[3KnYYPld{B1cGViYYX0c5Bp[We7IILheIlwyqB? MXuyNlE2PTF3MB?=
Marc-145 NIroVJdHfW6ldHnvckBCe3OjeR?= MmDvOY1O NH7tS4IyOi9{ND:zOog> MlqwdoVlfWOnczD0bIUhWFKUU2[geIl1\XK|IHHu[EB1cGVicILveIVqdiCneIDy[ZN{cW:w M4\3TVIzOTF7OUCw
HBx  NYDrfXg1SXCxcITvd4l{KEG|c3H5 NVOzNZpPOTCvTR?= MmD4OFhp NWLUOnZzTE2VTx?= MoKxbY5kemWjc3XzJINmdGxiZHXheIg> MWeyNlAzODB5OB?=
MCF-7 M3LnS2Z2dmO2aX;uJGF{e2G7 MYqxNI1O NI\xcW81QGh? M4LQNYJtd2OtczDheZRweGijZ4mgbY5lfWOnZDDifUBjd3K2ZYrvcYlj M2X4RVIyQTNzOUO3
RMPI8226  MYnGeY5kfGmxbjDBd5NigQ>? MWm1cW0> NX7ab|dLOWh? M3fafZN2eHC{ZYPz[ZMhfGinIHzleoVtKG:oIHH1eI9xcGGpeTD1coRmeiCwdYTybYVvfCCmZYDs[ZRqd25? NWDHOWxROjF7MUW2NlA>
PC12/TetOn Mm[zSpVv[3Srb36gRZN{[Xl? MoDrNE4yNzGvTR?= MnLmNVhp NX3tUllRdGWjZIOgeI8h|rFvc4nuLHdVMSCjY3P1cZVt[XSrb36sJJRwgGmlaYT5MEBidmRib3zp[49u\XJiZn;ycYF1cW:wwrC= M1zJcFIyQTB4NkW5
HeLa  NYfvOWxqS3m2b4TvfIlkcXS7IFHzd4F6 M4T2[FJuVQ>? NF;sVHIzPGh? M{fVdYlvcGmkaYTld{B1cGViY4n0c5RwgGmlaYT5JI9nKHOrbHnibY5qdiC2bzDI[WxiKGOnbHzzMi=> NV7TOld3OjF6N{WzPFU>
Jurkat MX3GeY5kfGmxbjDBd5NigQ>? M3vYVlExdU1? MUixbC=> NVf2W|NP\GWlcnXhd4V{KHSqZTDlfJBz\XO|aX;uJI9nKEyFMz3JTUBidmRidHjlJIZwem2jdHnvckBw\iCjdYTvdIhi\2:|b33ldy=> MmfKNlE5PjRyM{e=
K562 NYS3Z5BSTnWwY4Tpc44hSXO|YYm= MVexNI1O MnHqNYg> NH;xdFNl\WO{ZXHz[ZMhfGinIHX4dJJme3Orb36gc4YhVEN|LVnJJIFv\CC2aHWg[o9zdWG2aX;uJI9nKGG3dH;wbIFod3OxbXXz MW[yNVg3PDB|Nx?=
Nara-H MnX0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2DCZ|VuVQ>? MmfwOFhp M4LZN4VvcGGwY3XzJJRmdXOrcn;sbY12e22nZHnheIVlKHO3cIDy[ZN{cW:wIH;mJG5iemFvSDDj[YxtKHC{b3zp[oVz[XSrb36= NFX0coQzOThyNUCzNy=>
HUVECs M3\sTGZ2dmO2aX;uJGF{e2G7 NEfafmwyOG2P NWr2UmpKOC53aB?= NULySo9y\GWlcnXhd4V{KHSqZTDBS2UuSlODaX7keYNm\CCjdYTvdIhi\3libHX2[S=> NULvTYtmOjF2Nki1PVI>
HepG2 NWLjTWtoSXCxcITvd4l{KEG|c3H5 M1H3NVJuVQ>? NFPCcnkycA>? NGTJWm9mdmijbnPld{Bz[WSrYYTpc44ucW6mdXPl[EBk\WyuIHTlZZRp NYX4SHI3OjF2NUO2PVE>

... Click to View More Cell Line Experimental Data

In vivo 3-Methyladenine blocks autophagy through its effect on class III phosphatidylinositol 3-kinase (PI3K). 3-Methyladenine treatment does not alter the degree of hemorrhage compared with the subarachnoid hemorrhage (SAH) group. 3-Methyladenine pretreatment significantly aggravates neurological symptoms when compared with the SAH + vehicle group. Autophagy is decreased when 3-Methyladenine treatment is applied. Conversely, cleaved caspase-3 is markedly up-regulated in the SAH + 3-Methyladenine group. In line with the up-regulation of cleaved caspase-3 expression, the number of TUNEL-positive cells in the right cortex is significantly increased in the SAH + 3-Methyladenine group compared with the SAH + vehicle group. [5]

Protocol

Kinase Assay:[4]
+ Expand

Protein degradation assay:

HeLa cells are radiolabeled for 24 hours with 0.05 mCi/mL l-[U- 14C]valine. At the end of the labeling period, cells are rinsed three times with PBS. Cells are incubated for the designated times in either full medium or EBSS with or without the presence of 10 mM 3-Methyladenine.
Cell Research:[2]
+ Expand
  • Cell lines: HeLa cell line
  • Concentrations: 1-10 mM
  • Incubation Time: 24, 48 or 72 hours
  • Method: Cell (such as HeLa cell) viability is determined by a trypan blue exclusion assay. Briefly, after treated with 3-Methyladenine, both adherent and floating cells are collected and suspended in phosphate buffered saline (PBS, pH 7.4) at a final density of 1-2 × 106/mL. An equal volume of 0.4% trypan blue solution (w/v, in PBS) is added to the cell suspension and mixed thoroughly. After incubation at room temperature for 3 min, cell counting is performed using a hemacytometer.
    (Only for Reference)
Animal Research:[5]
+ Expand
  • Animal Models: Adult male Sprague–Dawley rats weighing 300-350 g
  • Formulation: Saline
  • Dosages: 400 nM
  • Administration: Intracerebral ventricular
    (Only for Reference)

Solubility (25°C)

In vitro Water 10 mg/mL warmed (67.04 mM)
Ethanol 4 mg/mL (26.81 mM)
DMSO 3 mg/mL warmed (20.11 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 149.15
Formula

C6H7N5

CAS No. 5142-23-4
Storage powder
in solvent
Synonyms NSC 66389

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    I'm also wondering whether it can be dissolved in water,or maybe something like culture medium,normal saline solution to form 10mM solution

  • Answer:

    As the reference (http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal. pone.0035665), 3-MA, which was found to inhibit autophagy at concentrations ranging from 1 to 10 mM was directly dissolved into the culture medium at the indicated concentrations. And we tested the solubility of S2767, and found the solubility of 3-MA in DMEM is 31 mg/mL at about 40°C.

PI3K Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID