3-Methyladenine (3-MA)

Catalog No.S2767 Synonyms: NSC 66389

3-Methyladenine (3-MA) Chemical Structure

Molecular Weight(MW): 149.15

3-Methyladenine (3-MA) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation.

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4 Customer Reviews

  • MDC-labeled vacuoles were induced by AZD2014 and inhibited by autophagy inhibitor (3-MA). SMMC-7721 cells were treated with AZD2014 or rapamycin at concentrations of 100 and 600 nM, respectively, for 48 hours in the presence or absence of 3-MA, and then stained with MDC. Cells were immediately observed under a confocal microscope. Cells in the control group were treated with DMSO. bars, 20 μm.

    Am J Cancer Res, 2015, 5(1): 125-139. 3-Methyladenine (3-MA) purchased from Selleck.

    Cells were treated with DMSO, TDCIPP (100 μM) only, or pretreated with 3-MA before treatment with TDCIPP (100 μM). LC3 conversion and beclin-1 and p62 expression were assessed by western blotting at 24 h

    Food Chem Toxicol, 2017, 100:183-196. 3-Methyladenine (3-MA) purchased from Selleck.

  • PC-9/ER cells were treated with CX-4945 (5 mM) for 48 h in the presence or absence of 3-MA (2 mM) and Atg7 siRNA (100 nM). Cleavage of PARP-1 and caspase-3 was shown by Western blot analysis. CT: without CX-4945; CX: CX-4945; CX+G: CX-4945 + gefitinib; CX+E: CX-4945 + erlotinib.

    PLoS One, 2014, 9(12): e114000 . 3-Methyladenine (3-MA) purchased from Selleck.

    A: The survival ratio of AML12 cells was measured after treatment with rapamycin or 3-MA; B: Western blot was used to detect the expression of LC3 and P62 in AML12 cells treated with Rapamycin or 3-MA.

    World J Gastroenterol, 2016, 22(18): 4501-4514.. 3-Methyladenine (3-MA) purchased from Selleck.

Purity & Quality Control

Choose Selective PI3K Inhibitors

Biological Activity

Description 3-Methyladenine (3-MA) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation.
Targets
Vps34 [1]
(HeLa cells)
PI3Kγ [1]
(HeLa cells)
25 μM 60 μM
In vitro

The slight preference for Vps34 prevention by 3-Methyladenine probably arises from a hydrophobic ring specific to Vps34, which encircles the 3-methyl group of 3-Methyladenine. [1] 3-Methyladenine has been reported to cause cancer cell death under both normal and starvation conditions. 3-Methyladenine could also suppress cell migration and invasion independently of its ability to inhibit autophagy, implying that 3-Methyladenine possesses functions other than autophagy suppression. 3-Methyladenine elicits caspase-dependent cell death that is independent of autophagy inhibition. Treatment with 5 mM 3-Methyladenine reduces the percentage of glucose-starved HeLa cells displaying GFP-LC3 puncta to 23%. The levels of LC3-I are increasing and the levels of LC3-II are decreasing between 12 and 48 hours in cells that are treated with 3-Methyladenine. Conversion of LC3-I to LC3-II is suppressed by 3-Methyladenine. Treatment of HeLa cells with 3-Methyladenine at 2.5 mM or 5 mM for one day does not affect cell viability, whereas treatment with 10 mM 3-Methyladenine for one day causes a 25.0% decrease in cell viability. Treatment of cells with 2.5, 5 or 10 mM 3-Methyladenine for two days causes 11.5%, 38.0% and 79.4% decrease in viability, respectively. 3-Methyladenine decreases cell viability in a time- and dose-dependent manner. 3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest. [2] Suppression of autophagy by 3-Methyladenine inhibits SU11274-induced cell death. [3] Prolonged treatment with 3-Methyladenine (up to 9 hours) induces significant LC3 I to II conversion in wild type MEFs. Prolonged treatment with 3-Methyladenine, but not wortmannin, markedly increases GFP-LC3 punctuation/aggregation. 3-Methyladenine-induced LC3 conversion and free GFP liberation are ATG7-dependent. 3-Methyladenine treatment leads to evident increase of p62 protein level. 3-Methyladenine increases the p62 level even in Atg5−/− MEFs as well as in cells with DOX-mediated deletion of ATG5. 3-Methyladenine inhibits class I and class III PI3K in different temporal patterns. 3-Methyladenine-induced LC3 I to LC3 II conversion is dramatically compromised in Tsc2−/− cells compared with wild type cells.3-Methyladenine disrupts the anti-autophagic function of mTOR complex 1. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
95D Mn;xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoWzN41O MlnZNog> NX;xfotmTE2VTx?= NWrWO5JGemWmdXPld{Boem:5dHigbY5pcWKrdH;yfUBm\m[nY4Sgc4YhSkSPQx?= MWCyOVcyPjV4MR?=
A549  MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWKzcW0> NWPufWNnOmh? NXi0VI15TE2VTx?= NYnibGh1emWmdXPld{Boem:5dHigbY5pcWKrdH;yfUBm\m[nY4Sgc4YhSkSPQx?= NFHTd|UzPTdzNkW2NS=>
95D NGLoXWlCeG:ydH;zbZMhSXO|YYm= Mn74OY1O NXLtO2xrOmh? NF7GSWlFVVOR MmXGbY5pcWKrdIOgRmROSy2rbnT1Z4VlKGGyb4D0c5Rq[yClZXzsJIRm[XSq NILiZZYzPTdzNkW2NS=>
A549  NGO3[nBCeG:ydH;zbZMhSXO|YYm= MoLHOY1O Mo\LNog> M3fHZWROW09? NEWxXFJqdmirYnn0d{BDTE2FLXnu[JVk\WRiYYDvdJRwfGmlIHPlcIwh\GWjdHi= NWXjNYZ4OjV5MU[1OlE>
NBL-W-S  NFfFPW5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFnmOYYydU1? M4P4NFZp MoLX[Y5p[W6lZYOgS2FPXC14MTD0c5hq[2m2eR?= MUGyOVMzOzJ{Mh?=
NBL-W-S  MY\BdI9xfG:|aYOgRZN{[Xl? MmHrNY1O MY[2bC=> MofibY5kemWjc3XzJINmdGxiYYDvdJRwe2m|IHnu[JVk\WRiYomgS2FPXC14MR?= M{HEeVI2OzJ|MkKy
A2780cp  MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYfHfYVsOW2P NWPHfVZvOWh? MmDsbY5kemWjc3XzJINqe3CuYYTpck1qdmS3Y3XkJINmdGxiZHXheIg> M{DBcVI2OzJ{Nkm0
A549  NXu0fVZkTnWwY4Tpc44hSXO|YYm= M4fKRlVuVQ>? NGLjNIIzcA>? M{\wN5N2eHC{ZYPz[ZMhW1CFIHnu[JVk\WRiYXPjeY12dGG2aX;uJI9nKEyFMz3JTS=> NYqxcHRiOjV{OEW2Nlg>
H157 NIXzc49HfW6ldHnvckBCe3OjeR?= M4nLSlVuVQ>? Mnf1Nog> NV\NNVFPe3WycILld5NmeyCVUFOgbY5lfWOnZDDhZ4N2dXWuYYTpc44hd2ZiTFOzMWlK MlHjNlUzQDV4Mki=
MDR M{XpZmFxd3C2b4Ppd{BCe3OjeR?= NFXxW|MyOG2P NVW4UXBtPmh? NVvUSotOe3S{ZX7neIhmdnNidHjlJJBwf2W{IH;mJIFvfGmlYX7j[ZIh\HK3Z4O= MX[yOVAyQTdyMR?=
HT-29  M3HTVmFxd3C2b4Ppd{BCe3OjeR?= Ml\RNY1O MYC0PIg> M3PUeWROW09? NHnXfZJmdmijbnPld{Bjd3K2ZYrvcYljNWmwZIXj[YQh[2WubDD2bYFjcWyrdImgcI9{ew>? M2q3d|I1QDR{MUW4
Microglia  NG\1fINCeG:ydH;zbZMhSXO|YYm= NWjY[o9RPW2P MnzJNlRp MULk[YNz\WG|ZYOgbJlxd3irYT3pcoR2[2WmIHPlcIwh\GWjdHi= NGDmd44zPDhzOE[wNS=>
A2780cp MX\BdI9xfG:|aYOgRZN{[Xl? M1fYfFIvPW2P NVnjfHE6OWh? M3H0WoRlUDKR Mnuy[Y5p[W6lZYOgZ4l{eGyjdHnuMYlv\HWlZXSgZ4VtdCCmZXH0bC=> NXjVcXBuOjR6MUe5OFY>
HepG2 Mk\YSpVv[3Srb36gRZN{[Xl? NFqxZ3A2dU1? NYW3VYFrPGh? NFHqNmhqdmO{ZXHz[ZMhUExicnXs[YF{\Q>? MVmyOFcyOzV6Nx?=
A549 MorCRZBweHSxc3nzJGF{e2G7 MXW1cW0> M4Tsb|Q5cA>? NIPafJFl\WO{ZXHz[ZMhfGinIIDldoNmdnSjZ3Wgc4Yh[2WubDDk[YF1cCCjbnSg[ZhxemW|c3nvckBt\X[nbIOgc4Yh[2G|cHHz[U0{NCCEZXPsbY4uOSCjbnSgUGM{NUmL NUXTO4hWOjR5ME[zNFM>
U2OS M4jCfmFxd3C2b4Ppd{BCe3OjeR?= M{fwTVExdU1? MknENlRp M3XHZ4lvfGWwc3nmbYV{KHSqZTDndo94fGhiaX7obYJqfGmxbjDv[kB1cGViVULPV{Bk\WyuczDpcoR2[2WmIHL5JGRwgA>? NXXrT2ZXOjR4M{mwNVM>
Saos-2 MUDBdI9xfG:|aYOgRZN{[Xl? M4DuUlExdU1? MXOyOIg> NFfRNJVqdnSnboPp[olmeyC2aHWg[5Jwf3SqIHnubIljcXSrb36gc4YhfGinIGWyU3Mh[2WubIOgbY5lfWOnZDDifUBFd3h? NX3HOnJOOjR4M{mwNVM>
A549 MX7BdI9xfG:|aYOgRZN{[Xl? MmfwNVBuVQ>? MXi0PIg> NVTIR4VJ[WOlZXzldoF1\XNidHjlJJJm\HWldHnvckBw\iClZXzsJJZq[WKrbHn0fUBqdmS3Y3XkJIJ6KFCWWB?= MUCyOFYzPjd{Mh?=
HCT116  NIPSWYNCeG:ydH;zbZMhSXO|YYm= MoDFOY1O MlTsNlRp M4PWNoVvcGGwY3XzJJRp\SCjcH;weI9{cXNiaX7keYNm\CCkeTDhdIlo\W6rbh?= NHjZdmYzPDZ{NkWyNi=>
HGC-27 M3XOUWZ2dmO2aX;uJGF{e2G7 MWexNI1O M1PabFFp MonXbY5pcWKrdIOgeIhmKGOnbHygeoli[mmuaYT5JIxwe3NiYomgVmFFODBzIH;yJG1MNTJ{ME[= MoDWNlQ1OTZ|NEm=
U2OS  NGnKTWtHfW6ldHnvckBCe3OjeR?= NIfkN5AxNjVxMX3N MmPDOFhp NICyTmNqdmS3Y3XzJJNidGmwb335Z4lvNWmwZIXj[YQh[2WubDD2bYFjcWyrdImgcI9{ew>? NF\OXI8zPDN3OEO0Ni=>
MG-63 Mm\ISpVv[3Srb36gRZN{[Xl? MmqyNE42NzGvTR?= NHTSTIM1QGh? NHjo[opqdmS3Y3XzJJNidGmwb335Z4lvNWmwZIXj[YQh[2WubDD2bYFjcWyrdImgcI9{ew>? NGTCN3gzPDN3OEO0Ni=>
MG-63 MnfRRZBweHSxc3nzJGF{e2G7 NXLycXlUOC53L{HtUS=> NV7ad4lEOzKq MUTlcohidmOnczDzZYxqdm:veXPpck1qdmS3Y3XkJINmdGxiYYDvdJRwe2m| M1G4PVI1OzV6M{Sy
MG-63 MmTGRZBweHSxc3nzJGF{e2G7 MWKxNI1O NFfWdY0yOmh? NEmweFlmdmijbnPld{BFWC2rbnT1Z4VlKGGyb4D0c5Nqew>? MWOyOFM2QDNyMR?=
NTUB1 MkPXRZBweHSxc3nzJGF{e2G7 MYC1cW0> M3XHZlEvPWh? M17lZZBwfGWwdHnheIV{KGOnbHXjc5hq[i2rbnT1Z4VlKGGyb4D0c5Nqew>? MkjPNlQ{PDlzN{[=
T24 M3S3eGFxd3C2b4Ppd{BCe3OjeR?= NVTkXYMzPW2P NUP1Z5BIOS53aB?= NXTJb2hKeG:2ZX70bYF1\XNiY3Xs[YNwgGmkLXnu[JVk\WRiYYDvdJRwe2m| MkPDNlQ{PDlzN{[=
SGC-7901  NFPJbGpCeG:ydH;zbZMhSXO|YYm= NIfycmEzdU1? MYixbC=> MVrpcoNz\WG|ZYOgR2EuPCCrbnT1Z4VlKGGyb4D0c5Nqew>? NV\yPZM2OjR|MkGzOFA>
SMMC-7721 Mkn0RZBweHSxc3nzJGF{e2G7 MoXtNo1O M4fhOFFp NH;vdpVqdmO{ZXHz[ZMhS0FvNDDpcoR2[2WmIHHwc5B1d3Orcx?= M1HTZVI1OzJzM{Sw
ECSCs  MojORZBweHSxc3nzJGF{e2G7 NVixVnYxOTCvTR?= MWC0bC=> MXvk[YNz\WG|ZYOgdoFx[W27Y3nuMZRz\WG2ZXSgZZBweHSxc3nz M2mxU|I1OzF7MUC5
MCF-7  MXLGeY5kfGmxbjDBd5NigQ>? NELHXIYyOG2P NEnpW20zPGh? MnrmbY5pcWKrdIOgeIhmKGG3dH;wbIFogSCrbnT1Z4VlKGK7IHPo[Y1wfGincnHwfUBlenWpcx?= NWjMN5FvOjR|MUW1O|g>
UOK257 NVnjUHZRSXCxcITvd4l{KEG|c3H5 NVqzUY5KPW2P MVezbC=> MYHlcohidmOnczDwZYNtcXSjeHXsMY1m\GmjdHXkJIFxd3C2b4Ppd:Kh NXXjN2Q3OjR|MEW2NFQ>
ACHN-5968 NIHoVJRCeG:ydH;zbZMhSXO|YYm= M3fPfFVuVQ>? NXG5OnFYO2h? NVvsU2hU\W6qYX7j[ZMheGGlbHn0ZZhmdC2vZXTpZZRm\CCjcH;weI9{cXQEoB?= MlXwNlQ{ODV4MES=
Huh7 Mn;URZBweHSxc3nzJGF{e2G7 MnPaNo1O NXvy[JYzOTKq Mnv4SG1UVw>? MlnZbY5pcWKrdIOgRXpFQDB3NT3pcoR2[2WmIHPlcIwh\GWjdHi= M4\MRVI1Ojl5M{Cw
Hep3B MmXMRZBweHSxc3nzJGF{e2G7 NFPlXW4zdU1? MUixNog> Ml76SG1UVw>? MX;pcohq[mm2czDBXmQ5ODV3LXnu[JVk\WRiY3XscEBl\WG2aB?= NFLoSYQzPDJ7N{OwNC=>
RKO MYnGeY5kfGmxbjDBd5NigQ>? MXSycW0> NYLmNJBNOWh? NX3WO4VjTE2VTx?= MVzlcohidmOnczDj[YxtKGSnYYToJIJ6KGenbHThcoFugWOrbh?= MVqyOFI6OTd5Nx?=
HepG2 E47 MXPGeY5kfGmxbjDBd5NigQ>? M2PVUlIvPW2P MkDZOFhp MVTpcoNz\WG|ZYOgeIhmKHSxeHnjbZR6KG:oIFHBMEBDW09uIHHu[EBES2x2 MlHDNlQzPzN5M{i=
LoVo  NWDTNpZ6SXCxcITvd4l{KEG|c3H5 NVfSOHJ{PW2P NECwb5g1QGh? M3TuTIVvcGGwY3XzJGRESS2rbnT1Z4VlKGGyb4D0c5Nqey5? M{H4dlI1OjBzOEGy
WiDr MUHGeY5kfGmxbjDBd5NigQ>? NYnYN3l1OTCvTR?= NE\rb5YycA>? MlPtbY5pcWKrdIOgVGNDVC2rbnT1Z4VlKEyFMzDJTUBmgHC{ZYPzbY9v M1fCO|I1OTlyNEi5
H1299  NV30ZZIzTnWwY4Tpc44hSXO|YYm= NIewOo4yOG2P NWj5TXd{PGh? Mm\3bY5kemWjc3XzJINqe3CuYYTpck1qdmS3Y3XkJINmdGxiZHXheIg> Mm\4NlQyPzN{MEi=
H460 M{j0SGZ2dmO2aX;uJGF{e2G7 MVWxNI1O MkDBOIg> NIPXT5BqdmO{ZXHz[ZMh[2m|cHzheIlvNWmwZIXj[YQh[2WubDDk[YF1cA>? MVOyOFE4OzJyOB?=
A549 MWPGeY5kfGmxbjDBd5NigQ>? M4XvUlExdU1? NHXqfFM1cA>? NVHVVpRZcW6qaXLpeJMh[XW2b4DoZYd6KGmwZIXj[YQh[nliaYLyZYRq[XSrb36= MYWyOFE1Ojd|NR?=
Hep3B MYDBdI9xfG:|aYOgRZN{[Xl? MYW1cW0> MkDvNlRp MmXXZZR1\W63YYTld{BVVkZvzsGgdJJwfGWldHnvckBi\2GrboP0JJNmenWvIIP0ZZJ3[XSrb36tcYVlcWG2ZXSgZZBweHSxc3nz NXTneppHOjRyNk[2PVM>
SMMC-7721 NELhfI9CeG:ydH;zbZMhSXO|YYm= MljQOY1O MWSyOIg> NV\pW|lY[XS2ZX71ZZRmeyCWTl[t{tEheHKxdHXjeIlwdiCjZ3HpcpN1KHOncoXtJJN1[XK4YYTpc44udWWmaXH0[YQh[XCxcITvd4l{ M3m2V|I1ODZ4Nkmz
HO8910 MojCRZBweHSxc3nzJGF{e2G7 MofoNVBuVQ>? NEXWPFkyOmh? NID6eYtmdmijbnPld{BDOTlvaX7keYNm\CCjcH;weI9{cQ>? MmLhNlM6QDN4MUC=
MCF7 MYDGeY5kfGmxbjDBd5NigQ>? MnruOY1O MkXHNlRp MX;pcoNz\WG|ZYOgR5VQKGmwZIXj[YQh[2WubDDk[YF1cA>? M3LFeVI{QTZ{NkK5
HONE-1  NVLEcHgyTnWwY4Tpc44hSXO|YYm= NWPlTYhqPW2P NUjyfGVCOWh? M1vZcpJmeHKnc4Pld{A3ei2vZXTpZZRm\CCUT2OgdJJw\HWldHnvci=> NXn4PXVTOjN6OUKzOVg>
HeLa NVXiPFBpTnWwY4Tpc44hSXO|YYm= Ml7QNVBuVQ>? MVSybC=> MYHzeZBxemW|c3XzJGxEOyCLSTDlfJBz\XO|aYPvci=> MX:yN|g3PDd|OB?=
HepG2 NHK5Uo1HfW6ldHnvckBCe3OjeR?= NGTmboIyOG2P NIDnUY0zPGh? NGmxU3NqdmirYnn0d{B{cVSLR1HSMUBidmRiSFLTV{1qdmS3Y3XkJIF2fG:yaHHnfS=> M1X4d|I{QDF5MESw
SH-SY5Y NIP5bFBHfW6ldHnvckBCe3OjeR?= NIjZTVIydU1? MkXjNlRp NEK2T2pqdmirYnn0d{B1cGViYYX0c5Bp[We7IHnu[JVk\WRiYomgWG9EWA>? M{nQd|I{PzR|MUS4
SH-SY5Y NFzTVIdCeG:ydH;zbZMhSXO|YYm= MlHBOY1O Ml3QNYg> MXThZo9tcXOqZYOgZ4Vt[XO2cn;sJI5mfXKxcILveIVkfGm4ZTDl[oZm[3R? NGjmdVIzOzZzOUO5OS=>
PC12  MV;GeY5kfGmxbjDBd5NigQ>? MVOxNI1O M3[4ZVI1cA>? M4PUfpdifGW{ M{XpbuKhcW6qaXLpeJMh[2i7bX;0dplxe2mwLXzpb4UheHKxdHXhd49u[WxiYXP0bZZqfHlw M{fTZlI{PjB|OUe5
OV2008 M2L1ZmFxd3C2b4Ppd{BCe3OjeR?= NH:0Vo42dU1? NF\wbJgzPGh? MkGzZ49vfmW{dIOgSnR[PzJyIIfpeIghS0SGUDDpcpRwKGGwIHHk[Il1cX[nIHXm[oVkfCC2b4fhdoR{KGurbHzpcochd3[jcnnhckBk[W6lZYKgZ4VtdHN? NGG4XJozOzV7MkK4NS=>
A2780 NVrFV2xHSXCxcITvd4l{KEG|c3H5 M3jE[|VuVQ>? NGPrOoQzPGh? NF7sbZBkd264ZYL0d{BHXFl5MkCge4l1cCCFRFTQJIlvfG9iYX6gZYRlcXSrdnWg[YZn\WO2IITve4Fz\HNia3nscIlv\yCxdnHybYFvKGOjbnPldkBk\Wyucx?= MUeyN|U6OjJ6MR?=
Saos-2  NVXIbXU6SXCxcITvd4l{KEG|c3H5 MVOxcW0> M4mzSVk3cA>? NGPyWmtqdmO{ZXHz[ZMh[2WubDDk[YF1cCCrbnT1Z4VlKGK7IGDDXC=> NXTRTZRSOjN3NkOxO|E>
1321N1 Mnr6R5l1d3SxeHnjbZR6KEG|c3H5 NXzrbFNKPW2P MYeyOIg> NI[xW2hxem:2ZXP0d{Bk\WyuIHHnZYlve3RiUFPOMYlv\HWlZXSgeI95cWOrdIm= MUmyN|UzPTJ4NR?=
SH-SY5Y MUXDfZRwfG:6aXPpeJkhSXO|YYm= NGTWZm82dU1? NVjOTJU5OjSq MVzpcoNz\WG|ZYOgVGNPKHSxeHnjbZR6 MVyyN|UzPTJ4NR?=
HT-29  MYXGeY5kfGmxbjDBd5NigQ>? NH;mZ2kydU1? MUK0PE86Pmh? MmDxbY5pcWKrdIOgRW1RUyCrbnT1Z4V{KGG3dH;wbIFocWNiY3XscEBl\WG2aB?= MUKyN|UxQDJ5Mh?=
OR6 NIfheI9HfW6ldHnvckBCe3OjeR?= M2HqUFExdU1? MVW3Nog> MWrzeZBxemW|c3XzJGhEXiC{ZYDsbYNifGmxbjDhcoQh\m:{bXH0bY9vKG:oIHH1eI9xcGGpb4PvcYV{ NEK4XW8zOzN7NUi3OS=>
Hela  NH;YbFJHfW6ldHnvckBCe3OjeR?= NHPOW4M2dU1? MY[yOIg> Mli0bY5pcWKrdIOgd5Rien[jdHnvck1qdmS3Y3XkJIF2fG:yaHHnfS=> MnuxNlM{QTV4N{m=
MCF-7  M3\oSWZ2dmO2aX;uJGF{e2G7 NGXhPFI2dU1? MnfmNlRp NEG5VpVqdmirYnn0d{B{fGG{dnH0bY9vNWmwZIXj[YQh[XW2b4DoZYd6 NUfnOlJCOjN|OUW2O|k>
HUVECs NWLZcWEzTnWwY4Tpc44hSXO|YYm= NYXSNVZrO22P M4DRZ|I1cA>? NXXMbYpp[myxY3vzJJRp\SCycn;0[YN1cX[nIHXm[oVkfCCxZjDy[ZN3\XKjdILvcEBjgSCrbnjpZol1cW6pIHH1eI9xcGGpeR?= MmfiNlM{PTh7Mki=
T24 NF\1NGNHfW6ldHnvckBCe3OjeR?= NVfTcmJMOTCvTR?= NIW5VIoycA>? MXXy[YR2[2W|IITo[UBkdGWjdnHn[UBw\iCOQ{OgZYZ1\XJiYnHpZ4FtcW5idILlZZRu\W62 MWWyN|M2PDB6MB?=
U251MG  NXvXTpUxTnWwY4Tpc44hSXO|YYm= NX7WT5QzO22P NUD2PZdWOWh? Ml[5d5VxeHKnc4Pld{BNSzNvSVmgdJJwfGWrbjDlfJBz\XO|aX;u NIf1NnIzOzN|OE[xPC=>
GTL-16  M1vxcmFxd3C2b4Ppd{BCe3OjeR?= Mn7JOY1O MnTzNlRp MkfvdoVlfWOnczDj[YxtKH[rYXLpcIl1gSCjczDjc41x[XKnZDD0c{Bk\WyuczD0doVifGWmIIfpeIghVUWWIHnubIljcXSxcoO= NHHTVFkzOzNzM{S5NC=>
T-47D MlnTSpVv[3Srb36gRZN{[Xl? MXWxNI1O MnXJNog> Ml3abY5pcWKrdIOgZZV1d3CqYXf5JJBzd2Onc4OgZY5lKGmwY4LlZZNmeyC{YYDhcZlkcW5iaX7keYNm\CCjcH;weI9{cXN? MXuyN|MxODB{Nh?=
PaCa44 MkP1RZBweHSxc3nzJGF{e2G7 NV;PSItIOi53bV2= MlfsNYg> M1\CR5Jm\HWlZYRCpIdmdmmyaX6tcYVlcWG2ZXSgZZBweHSxc3nz MXSyN|EzPDFzMh?=
MDA-MB231 M33kdWZ2dmO2aX;uJGF{e2G7 NGr5ZVk2dU1? MljFNYg> NEnNcYJqdmO{ZXHz[ZMhemW|dnXyZZRzd2xvbXXkbYF1\WRiY3HzdIF{\SCjY4TpeoF1cW:wIHHu[EBk\WyuIHTlZZRp M4XBVVI{ODh6OEWw
HeLa MkT5RZBweHSxc3nzJGF{e2G7 MorrNVBuVQ>? M1vUb|Jp MVHk[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliY3:teJJm[XSvZX70JJdqfGhiUFXJ M3v6UlI{ODByMUO1
SK-HEP-1 NHzyNXJCeG:ydH;zbZMhSXO|YYm= M3zzS|ExdU1? MVOxbC=> MXvwdo91\WO2czDh[4FqdnO2IHH1eI9xcGGpeTDhcoQhcW6mdXPld{BieG:ydH;zbZMhcW5iYoXmZYxqdi22cnXheIVlKGOnbHzz NUew[mlKOjJ6NUi2OFk>
HepG2 M17icGFxd3C2b4Ppd{BCe3OjeR?= NUHrWpBMO22P NIX3fG82cA>? MXLy[YR2[2W|IHPlcIwh[XCxcITvd4l{KGmwZIXj[YQh[nliUVTz NH2xXFMzOjh|NkW5OS=>
MCF-7  M2K5OGZ2dmO2aX;uJGF{e2G7 MVyycW0> NFjCOWozPGh? NEKx[YVqdmirYnn0d{BifXSxcHjh[5khcW6mdXPl[EBjgSCWTdMg M1HQS|I1QTdyNke2
MDA-MB-231 NVHrSm5ZTnWwY4Tpc44hSXO|YYm= M2PBTVJuVQ>? M4L3UlI1cA>? NIW5T3ZqdmirYnn0d{BifXSxcHjh[5khcW6mdXPl[EBjgSCWTdMg M{PHV|I1QTdyNke2
MCF-7  NGG3bldHfW6ldHnvckBCe3OjeR?= NG[1[G8zdU1? NUD6b5REPDiq NI[yPIlxem:vb4Tld{BVVS2rbnT1Z4VlKGOnbHyg[IVifGh? MXqyOFk4ODZ5Nh?=
MDA-MB-231 NEm2UlJHfW6ldHnvckBCe3OjeR?= MUWycW0> NVLsNpIxPDiq NUCzfXFyeHKxbX;0[ZMhXE1vaX7keYNm\CClZXzsJIRm[XSq NYjZ[mZNOjR7N{C2O|Y>
PANC-1  NV35O2h3SXCxcITvd4l{KEG|c3H5 NF\nXG8ydU1? MXi0PIg> M3P1eWROW09? MWHlcohidmOnczDic5J1\XqxbXniMYlv\HWlZXSgZ4VtdCC4aXHibYxqfHlibH;zdy=> MWSyOFg1OjF3OB?=
MDA-MB 231 NHO2SHZCeG:ydH;zbZMhSXO|YYm= M1qyVlVuVQ>? NFTMWI8xNjWq MYftc4R2dGG2ZYOgWI9kd22rbtMuxsBqdmS3Y3XkJIFxd3C2b4Ppdy=> NH3GW4wzPDh|MEe4NS=>
Microglia  NXLSS4Z2SXCxcITvd4l{KEG|c3H5 MXe1cW0> NHG4NmszPGh? NIPNVHhl\WO{ZXHz[ZMhcHmyb4jpZU1qdmS3Y3XkJINmdGxiZHXheIg> M4nKd|I1QDF6NkCx
A2780cp NHrXN2RCeG:ydH;zbZMhSXO|YYm= Ml;3Nk42dU1? MVexbC=> MWjk[GgzVw>? M4TtU4VvcGGwY3XzJINqe3CuYYTpck1qdmS3Y3XkJINmdGxiZHXheIg> MXeyOFgyPzl2Nh?=
HepG2 M{fIdWZ2dmO2aX;uJGF{e2G7 MUe1cW0> MmfHOIg> NHXQe4xqdmO{ZXHz[ZMh[2WubIXsZZIhdGW4ZXzzJI9nKEiOwrC= MkS4NlQ4OTN3OEe=
U2OS MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFnsW4MyOG2P MUKyOIg> NHP2dmhqdnSnboPp[olmeyC2aHWg[5Jwf3SqIHnubIljcXSrb36gbY5lfWOnZDDifUBFd3h? MkC3NlQ3OzlyMUO=
HCT116 NIe1[W5CeG:ydH;zbZMhSXO|YYm= NWfVboNbPW2P NWrNNXhQOjSq MYrEUXNQ NXTWbVhb\W6qYX7j[ZMh[XCrZ3XubY4ucW6mdXPl[EBk\WyuIHTlZZRp MmTiNlQ3OjZ3MkK=
MCF-7  MmjpRZBweHSxc3nzJGF{e2G7 MmTyNE4ydU1? MYC2bC=> M17ocoVvcGGwY3XzJJNqenSrbn;sMYlv\HWlZXSgZZBweHSxc3nz M4XpblIzPzVzOUi5
PC-3  Ml\FRZBweHSxc3nzJGF{e2G7 Mm\rNo1O MYiybC=> MoWybY5kemWjc3XzJG9TUS2rbnT1Z4VlKGOnbHyg[IVifGh? NULNclJSOjJ5NEW1PFA>
U251  NH;OUVdCeG:ydH;zbZMhSXO|YYm= MVi1cW0> NUnXWnVEOjSq MYLpcoNz\WG|ZYOgV|EucW6mdXPl[EBk\WyuIHTlZZRp NX3lNlV3OjJ3N{m3PFg>
HeLa  MmLyRZBweHSxc3nzJGF{e2G7 MoW3OY1O M17NWVI1cA>? M1;2S4lv\HWlZYOgZ4F{eGG|ZT3k[ZBmdmSnboSgZ4VtdCCmZXH0bC=> MXOyNlU1PTF{OB?=
A549 NYPWe4FGTnWwY4Tpc44hSXO|YYm= MoWyNE4ydU1? M13BS|I1cA>? NX\DVZV6e3WycILld5NmeyCVVUGxNlc1NWmwZIXj[YQh[2WubDDk[YF1cA>? NEDvclQzOjR4Nkm2NC=>
pDCs M1jueWZ2dmO2aX;uJGF{e2G7 MWSxNI1O MonaNE42cA>? MVlCpJJm\HWlZYOgeIhmKGmwZIXjeIlwdiCxZjDJSm4u|rFiYomgd5NTVkF2MB?= MWKyNlM6PjV7OR?=
BGC-823 MmP1SpVv[3Srb36gRZN{[Xl? NEfFWIY2dU1? NV7r[|d1Omh? NXHrRWUxcW6qaXLpeJMhfGinIILheIUhd2ZiYYX0c5Bp[WerYzDj[Yxtew>? NGjXVFMzOjN{MkG1Ni=>
U937 NYq0eJJDTnWwY4Tpc44hSXO|YYm= NGXCXVQzdU1? M2nOPFEzcA>? M{XWb4Rm[3KnYYPld{B1cGViYYX0c5Bp[We7IILheIlwyqB? MkLtNlIyPTVzNUC=
Marc-145 NVnE[WlDTnWwY4Tpc44hSXO|YYm= NXTtcmNxPW2P M4PhOFEzNzJ2L{O2bC=> M2i3VpJm\HWlZYOgeIhmKFCUUmPWJJRqfGW{czDhcoQhfGinIIDyc5RmcW5iZYjwdoV{e2mxbh?= NYGyeI1rOjJzMUm5NFA>
HBx  NX7PPVhKSXCxcITvd4l{KEG|c3H5 NXS4eJFUOTCvTR?= NXK1TZBIPDiq NFPKcI9FVVOR NHXyb29qdmO{ZXHz[ZMh[2WubDDk[YF1cA>? NHO2fnQzOjB{MEC3PC=>
MCF-7 NWCxPGFiTnWwY4Tpc44hSXO|YYm= NUfjd4FyOTCvTR?= Mly4OFhp NYrYe2tm[myxY3vzJIF2fG:yaHHnfUBqdmS3Y3XkJIJ6KGKxcoTlfo9ucWJ? NGnCcYwzOTl|MUmzOy=>
RMPI8226  M1riXGZ2dmO2aX;uJGF{e2G7 NU\YU3RTPW2P M3XNcFFp M13IeJN2eHC{ZYPz[ZMhfGinIHzleoVtKG:oIHH1eI9xcGGpeTD1coRmeiCwdYTybYVvfCCmZYDs[ZRqd25? M33Ve|IyQTF3NkKw
PC12/TetOn M1rKU2Z2dmO2aX;uJGF{e2G7 NFi4bVgxNjFxMX3N MX6xPIg> NEPzNIVt\WGmczD0c{DPuS2|eX6oW3QqKGGlY4XteYxifGmxbjygeI95cWOrdImsJIFv\CCxbHnnc41meiCob4LtZZRqd28EoB?= NYL1UG9NOjF7ME[2OVk>
HeLa  NGHFfGREgXSxdH;4bYNqfHliQYPzZZk> M1HON|JuVQ>? MmThNlRp M{jXd4lvcGmkaYTld{B1cGViY4n0c5RwgGmlaYT5JI9nKHOrbHnibY5qdiC2bzDI[WxiKGOnbHzzMi=> NUjRWVQ{OjF6N{WzPFU>
Jurkat MVnGeY5kfGmxbjDBd5NigQ>? MoLYNVBuVQ>? M{XFTlFp MUTk[YNz\WG|ZYOgeIhmKGW6cILld5Nqd25ib3[gUGM{NUmLIHHu[EB1cGViZn;ycYF1cW:wIH;mJIF2fG:yaHHnc5NwdWW| MnnSNlE5PjRyM{e=
K562 MX;GeY5kfGmxbjDBd5NigQ>? MlXRNVBuVQ>? MmXvNYg> M4HOXIRm[3KnYYPld{B1cGViZYjwdoV{e2mxbjDv[kBNSzNvSVmgZY5lKHSqZTDmc5Ju[XSrb36gc4Yh[XW2b4DoZYdwe2:vZYO= Ml3lNlE5PjRyM{e=
Nara-H NWLGcpF1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF\F[2U2dU1? M3jablQ5cA>? MljE[Y5p[W6lZYOgeIVue2m{b3zpcZV{dWWmaXH0[YQhe3WycILld5Nqd25ib3[gUoFz[S2KIHPlcIwheHKxbHnm[ZJifGmxbh?= NV7GOo1QOjF6MEWwN|M>
HUVECs NEnwO3BHfW6ldHnvckBCe3OjeR?= M1zrTVExdU1? MVmwMlVp MXjk[YNz\WG|ZYOgeIhmKEGJRT3CV2FqdmS3Y3XkJIF2fG:yaHHnfUBt\X[n NHv2Zm4zOTR4OEW5Ni=>
HepG2 M4DoO2Fxd3C2b4Ppd{BCe3OjeR?= M2\LSlJuVQ>? NXjKZ4xiOWh? M2S5e4VvcGGwY3XzJJJi\GmjdHnvck1qdmS3Y3XkJINmdGxiZHXheIg> NYrUe2xnOjF2NUO2PVE>

... Click to View More Cell Line Experimental Data

In vivo 3-Methyladenine blocks autophagy through its effect on class III phosphatidylinositol 3-kinase (PI3K). 3-Methyladenine treatment does not alter the degree of hemorrhage compared with the subarachnoid hemorrhage (SAH) group. 3-Methyladenine pretreatment significantly aggravates neurological symptoms when compared with the SAH + vehicle group. Autophagy is decreased when 3-Methyladenine treatment is applied. Conversely, cleaved caspase-3 is markedly up-regulated in the SAH + 3-Methyladenine group. In line with the up-regulation of cleaved caspase-3 expression, the number of TUNEL-positive cells in the right cortex is significantly increased in the SAH + 3-Methyladenine group compared with the SAH + vehicle group. [5]

Protocol

Kinase Assay:[4]
+ Expand

Protein degradation assay:

HeLa cells are radiolabeled for 24 hours with 0.05 mCi/mL l-[U- 14C]valine. At the end of the labeling period, cells are rinsed three times with PBS. Cells are incubated for the designated times in either full medium or EBSS with or without the presence of 10 mM 3-Methyladenine.
Cell Research:[2]
+ Expand
  • Cell lines: HeLa cell line
  • Concentrations: 1-10 mM
  • Incubation Time: 24, 48 or 72 hours
  • Method: Cell (such as HeLa cell) viability is determined by a trypan blue exclusion assay. Briefly, after treated with 3-Methyladenine, both adherent and floating cells are collected and suspended in phosphate buffered saline (PBS, pH 7.4) at a final density of 1-2 × 106/mL. An equal volume of 0.4% trypan blue solution (w/v, in PBS) is added to the cell suspension and mixed thoroughly. After incubation at room temperature for 3 min, cell counting is performed using a hemacytometer.
    (Only for Reference)
Animal Research:[5]
+ Expand
  • Animal Models: Adult male Sprague–Dawley rats weighing 300-350 g
  • Formulation: Saline
  • Dosages: 400 nM
  • Administration: Intracerebral ventricular
    (Only for Reference)

Solubility (25°C)

In vitro Water 10 mg/mL warmed (67.04 mM)
Ethanol 4 mg/mL (26.81 mM)
DMSO 3 mg/mL warmed (20.11 mM)
In vivo Add solvents to the product individually and in order:
water
For best results, use promptly after mixing.
10mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 149.15
Formula

C6H7N5

CAS No. 5142-23-4
Storage powder
Synonyms NSC 66389

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
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Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    I'm also wondering whether it can be dissolved in water,or maybe something like culture medium,normal saline solution to form 10mM solution

  • Answer:

    As the reference (http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal. pone.0035665), 3-MA, which was found to inhibit autophagy at concentrations ranging from 1 to 10 mM was directly dissolved into the culture medium at the indicated concentrations. And we tested the solubility of S2767, and found the solubility of 3-MA in DMEM is 31 mg/mL at about 40°C.

PI3K Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID