3-Methyladenine (3-MA)

Catalog No.S2767 Synonyms: NSC 66389

3-Methyladenine (3-MA) Chemical Structure

Molecular Weight(MW): 149.15

3-Methyladenine (3-MA) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation.

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4 Customer Reviews

  • MDC-labeled vacuoles were induced by AZD2014 and inhibited by autophagy inhibitor (3-MA). SMMC-7721 cells were treated with AZD2014 or rapamycin at concentrations of 100 and 600 nM, respectively, for 48 hours in the presence or absence of 3-MA, and then stained with MDC. Cells were immediately observed under a confocal microscope. Cells in the control group were treated with DMSO. bars, 20 μm.

    Am J Cancer Res, 2015, 5(1): 125-139. 3-Methyladenine (3-MA) purchased from Selleck.

    Cells were treated with DMSO, TDCIPP (100 μM) only, or pretreated with 3-MA before treatment with TDCIPP (100 μM). LC3 conversion and beclin-1 and p62 expression were assessed by western blotting at 24 h

    Food Chem Toxicol, 2017, 100:183-196. 3-Methyladenine (3-MA) purchased from Selleck.

  • PC-9/ER cells were treated with CX-4945 (5 mM) for 48 h in the presence or absence of 3-MA (2 mM) and Atg7 siRNA (100 nM). Cleavage of PARP-1 and caspase-3 was shown by Western blot analysis. CT: without CX-4945; CX: CX-4945; CX+G: CX-4945 + gefitinib; CX+E: CX-4945 + erlotinib.

    PLoS One, 2014, 9(12): e114000 . 3-Methyladenine (3-MA) purchased from Selleck.

    A: The survival ratio of AML12 cells was measured after treatment with rapamycin or 3-MA; B: Western blot was used to detect the expression of LC3 and P62 in AML12 cells treated with Rapamycin or 3-MA.

    World J Gastroenterol, 2016, 22(18): 4501-4514.. 3-Methyladenine (3-MA) purchased from Selleck.

Purity & Quality Control

Choose Selective PI3K Inhibitors

Biological Activity

Description 3-Methyladenine (3-MA) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation.
Targets
Vps34 [1]
(HeLa cells)
PI3Kγ [1]
(HeLa cells)
25 μM 60 μM
In vitro

The slight preference for Vps34 prevention by 3-Methyladenine probably arises from a hydrophobic ring specific to Vps34, which encircles the 3-methyl group of 3-Methyladenine. [1] 3-Methyladenine has been reported to cause cancer cell death under both normal and starvation conditions. 3-Methyladenine could also suppress cell migration and invasion independently of its ability to inhibit autophagy, implying that 3-Methyladenine possesses functions other than autophagy suppression. 3-Methyladenine elicits caspase-dependent cell death that is independent of autophagy inhibition. Treatment with 5 mM 3-Methyladenine reduces the percentage of glucose-starved HeLa cells displaying GFP-LC3 puncta to 23%. The levels of LC3-I are increasing and the levels of LC3-II are decreasing between 12 and 48 hours in cells that are treated with 3-Methyladenine. Conversion of LC3-I to LC3-II is suppressed by 3-Methyladenine. Treatment of HeLa cells with 3-Methyladenine at 2.5 mM or 5 mM for one day does not affect cell viability, whereas treatment with 10 mM 3-Methyladenine for one day causes a 25.0% decrease in cell viability. Treatment of cells with 2.5, 5 or 10 mM 3-Methyladenine for two days causes 11.5%, 38.0% and 79.4% decrease in viability, respectively. 3-Methyladenine decreases cell viability in a time- and dose-dependent manner. 3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest. [2] Suppression of autophagy by 3-Methyladenine inhibits SU11274-induced cell death. [3] Prolonged treatment with 3-Methyladenine (up to 9 hours) induces significant LC3 I to II conversion in wild type MEFs. Prolonged treatment with 3-Methyladenine, but not wortmannin, markedly increases GFP-LC3 punctuation/aggregation. 3-Methyladenine-induced LC3 conversion and free GFP liberation are ATG7-dependent. 3-Methyladenine treatment leads to evident increase of p62 protein level. 3-Methyladenine increases the p62 level even in Atg5−/− MEFs as well as in cells with DOX-mediated deletion of ATG5. 3-Methyladenine inhibits class I and class III PI3K in different temporal patterns. 3-Methyladenine-induced LC3 I to LC3 II conversion is dramatically compromised in Tsc2−/− cells compared with wild type cells.3-Methyladenine disrupts the anti-autophagic function of mTOR complex 1. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
95D Mnv2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoiwN41O MX:ybC=> MlqzSG1UVw>? MmXOdoVlfWOnczDndo94fGhiaX7obYJqfG:{eTDl[oZm[3Rib3[gRmROSw>? M4Gzc|I2PzF4NU[x
A549  MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkLzN41O MWiybC=> Mo\USG1UVw>? M1XBOJJm\HWlZYOg[5Jwf3SqIHnubIljcXSxcomg[YZn\WO2IH;mJGJFVUN? NFy4dJgzPTdzNkW2NS=>
95D NG\zPJdCeG:ydH;zbZMhSXO|YYm= MUm1cW0> MkD1Nog> MWPEUXNQ MoDlbY5pcWKrdIOgRmROSy2rbnT1Z4VlKGGyb4D0c5Rq[yClZXzsJIRm[XSq NFjRepczPTdzNkW2NS=>
A549  MXLBdI9xfG:|aYOgRZN{[Xl? M3zoNlVuVQ>? MoP1Nog> MWDEUXNQ MlTIbY5pcWKrdIOgRmROSy2rbnT1Z4VlKGGyb4D0c5Rq[yClZXzsJIRm[XSq MXiyOVcyPjV4MR?=
NBL-W-S  MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnH4NY1O NWj4NZI4Pmh? NUjHeWV[\W6qYX7j[ZMhT0GQVD22NUB1d3irY3n0fS=> NVjlVGNmOjV|MkOyNlI>
NBL-W-S  NUjrO5RzSXCxcITvd4l{KEG|c3H5 M4e3R|FuVQ>? NIXFTJE3cA>? MkLUbY5kemWjc3XzJINmdGxiYYDvdJRwe2m|IHnu[JVk\WRiYomgS2FPXC14MR?= MXuyOVMzOzJ{Mh?=
A2780cp  MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{j5OFFuVQ>? M3XQO|Fp NULaWXVOcW6lcnXhd4V{KGOrc4DsZZRqdi2rbnT1Z4VlKGOnbHyg[IVifGh? MUCyOVMzOjZ7NB?=
A549  NWnnSXhSTnWwY4Tpc44hSXO|YYm= M3nyT|VuVQ>? M4nrOVJp MnjYd5VxeHKnc4Pld{BUWENiaX7keYNm\CCjY3P1cZVt[XSrb36gc4YhVEN|LVnJ NInRNYIzPTJ6NU[yPC=>
H157 MkHmSpVv[3Srb36gRZN{[Xl? MYq1cW0> M{nu[FJp M3vtcZN2eHC{ZYPz[ZMhW1CFIHnu[JVk\WRiYXPjeY12dGG2aX;uJI9nKEyFMz3JTS=> NX\WO5dnOjV{OEW2Nlg>
MDR M1j5OmFxd3C2b4Ppd{BCe3OjeR?= NHLYV2MyOG2P MoTQOog> NVv0Vnh{e3S{ZX7neIhmdnNidHjlJJBwf2W{IH;mJIFvfGmlYX7j[ZIh\HK3Z4O= NVzIc|BKOjVyMUm3NFE>
HT-29  MknYRZBweHSxc3nzJGF{e2G7 MXOxcW0> M3vUVlQ5cA>? NG\M[ZlFVVOR M3HhUoVvcGGwY3XzJIJwenSnen;tbYIucW6mdXPl[EBk\WyuII\pZYJqdGm2eTDsc5N{ MVWyOFg1OjF3OB?=
Microglia  Mm\WRZBweHSxc3nzJGF{e2G7 MYe1cW0> MoLYNlRp NX3rbm4z\GWlcnXhd4V{KGi7cH;4bYEucW6mdXPl[EBk\WyuIHTlZZRp NWXNU2I6OjR6MUi2NFE>
A2780cp MXjBdI9xfG:|aYOgRZN{[Xl? NVOwVVNJOi53bV2= NFPoXHYycA>? MkHO[IRJOk9? MlTQ[Y5p[W6lZYOgZ4l{eGyjdHnuMYlv\HWlZXSgZ4VtdCCmZXH0bC=> M4TyZVI1QDF5OUS2
HepG2 MkLuSpVv[3Srb36gRZN{[Xl? NIjufFM2dU1? MXe0bC=> Mne4bY5kemWjc3XzJGhNKHKnbHXhd4U> NVPzOWt5OjR5MUO1PFc>
A549 NIHMWppCeG:ydH;zbZMhSXO|YYm= MYm1cW0> NXXvbVBOPDiq NV\KdW9v\GWlcnXhd4V{KHSqZTDw[ZJk\W62YXflJI9nKGOnbHyg[IVifGhiYX7kJIV5eHKnc4Ppc44hdGW4ZXzzJI9nKGOjc4Dhd4UuOyxiQnXjcIlvNTFiYX7kJGxEOy2LSR?= MVmyOFcxPjNyMx?=
U2OS MXTBdI9xfG:|aYOgRZN{[Xl? NHvOeGsyOG2P MnGyNlRp M4PoRolvfGWwc3nmbYV{KHSqZTDndo94fGhiaX7obYJqfGmxbjDv[kB1cGViVULPV{Bk\WyuczDpcoR2[2WmIHL5JGRwgA>? M2S0UVI1PjN7MEGz
Saos-2 M{LqPGFxd3C2b4Ppd{BCe3OjeR?= MnflNVBuVQ>? MWGyOIg> MWnpcpRmdnOrZnnld{B1cGViZ4Lve5RpKGmwaHnibZRqd25ib3[geIhmKFV{T2OgZ4VtdHNiaX7keYNm\CCkeTDEc5g> M1G4clI1PjN7MEGz
A549 NHr2bFBCeG:ydH;zbZMhSXO|YYm= NWnmfmxEOTCvTR?= MlH0OFhp MmjYZYNk\WyncnH0[ZMhfGinIILl[JVkfGmxbjDv[kBk\WyuII\pZYJqdGm2eTDpcoR2[2WmIHL5JHBVYA>? M4jGNVI1PjJ4N{Ky
HCT116  MUPBdI9xfG:|aYOgRZN{[Xl? NUL3SFdtPW2P MVeyOIg> NYrCXHpV\W6qYX7j[ZMhfGinIHHwc5B1d3OrczDpcoR2[2WmIHL5JIFxcWenbnnu M{e0blI1PjJ4NUKy
HGC-27 MVfGeY5kfGmxbjDBd5NigQ>? Mlm2NVBuVQ>? NV7CN4w1OWh? NV7MfG95cW6qaXLpeJMhfGinIHPlcIwhfmmjYnnsbZR6KGyxc4OgZpkhWkGGMECxJI9zKE2NLUKyNFY> MWmyOFQyPjN2OR?=
U2OS  MWHGeY5kfGmxbjDBd5NigQ>? M2HDblAvPS9zbV2= MlfyOFhp NHnidVNqdmS3Y3XzJJNidGmwb335Z4lvNWmwZIXj[YQh[2WubDD2bYFjcWyrdImgcI9{ew>? NXPyNplsOjR|NUizOFI>
MG-63 NIPZfFhHfW6ldHnvckBCe3OjeR?= NYD4flZVOC53L{HtUS=> NF[3S2o1QGh? NEPCe29qdmS3Y3XzJJNidGmwb335Z4lvNWmwZIXj[YQh[2WubDD2bYFjcWyrdImgcI9{ew>? NYjJcYFnOjR|NUizOFI>
MG-63 Mn\hRZBweHSxc3nzJGF{e2G7 NHW3OngxNjVxMX3N NFjP[Y4{Omh? MV;lcohidmOnczDzZYxqdm:veXPpck1qdmS3Y3XkJINmdGxiYYDvdJRwe2m| NHz1XHUzPDN3OEO0Ni=>
MG-63 M{fGVWFxd3C2b4Ppd{BCe3OjeR?= NV;v[mE4OTCvTR?= MmjONVJp NXzyWmU1\W6qYX7j[ZMhTFBvaX7keYNm\CCjcH;weI9{cXN? NHXpTpYzPDN3OEOwNS=>
NTUB1 M2P3c2Fxd3C2b4Ppd{BCe3OjeR?= MnfaOY1O NYnzbpg4OS53aB?= MVfwc5RmdnSrYYTld{Bk\WynY3;4bYIucW6mdXPl[EBieG:ydH;zbZM> NIHGd5AzPDN2OUG3Oi=>
T24 NH\OPFFCeG:ydH;zbZMhSXO|YYm= MkHhOY1O NVqySmJFOS53aB?= NXHPXXZPeG:2ZX70bYF1\XNiY3Xs[YNwgGmkLXnu[JVk\WRiYYDvdJRwe2m| NX7heW5COjR|NEmxO|Y>
SGC-7901  MY\BdI9xfG:|aYOgRZN{[Xl? NFX6dG8zdU1? NUHvUJJtOWh? NWr6[WZEcW6lcnXhd4V{KEODLUSgbY5lfWOnZDDhdI9xfG:|aYO= M{i4elI1OzJzM{Sw
SMMC-7721 M1vy[GFxd3C2b4Ppd{BCe3OjeR?= NV3QcoZbOm2P NUTLT2NoOWh? MkHkbY5kemWjc3XzJGNCNTRiaX7keYNm\CCjcH;weI9{cXN? NH;NfJIzPDN{MUO0NC=>
ECSCs  M2OwemFxd3C2b4Ppd{BCe3OjeR?= NYPTRmw3OTCvTR?= M13PZ|Rp MVrk[YNz\WG|ZYOgdoFx[W27Y3nuMZRz\WG2ZXSgZZBweHSxc3nz NVXjbWMxOjR|MUmxNFk>
MCF-7  MY\GeY5kfGmxbjDBd5NigQ>? Mn3jNVBuVQ>? NYLRU5VJOjSq MorzbY5pcWKrdIOgeIhmKGG3dH;wbIFogSCrbnT1Z4VlKGK7IHPo[Y1wfGincnHwfUBlenWpcx?= M3TOSFI1OzF3NUe4
UOK257 NFnn[pFCeG:ydH;zbZMhSXO|YYm= MWG1cW0> M4[xbVNp NG\DVmtmdmijbnPld{Bx[WOuaYThfIVtNW2nZHnheIVlKGGyb4D0c5Nqe8Li NI\QVGwzPDNyNU[wOC=>
ACHN-5968 NI\GfpJCeG:ydH;zbZMhSXO|YYm= NYnuUVV5PW2P MUmzbC=> MlzS[Y5p[W6lZYOgdIFkdGm2YYjlcE1u\WSrYYTl[EBieG:ydH;zbZPDqA>? NXPvSnNqOjR|MEW2NFQ>
Huh7 NFfmdJNCeG:ydH;zbZMhSXO|YYm= MUOycW0> NY\4Vnh4OTKq NHj2[ndFVVOR M4W2bolvcGmkaYTzJGFbTDhyNUWtbY5lfWOnZDDj[YxtKGSnYYTo NFS5eWgzPDJ7N{OwNC=>
Hep3B NFrIbG9CeG:ydH;zbZMhSXO|YYm= Ml61No1O MkPWNVJp NVnxToh1TE2VTx?= MnnBbY5pcWKrdIOgRXpFQDB3NT3pcoR2[2WmIHPlcIwh\GWjdHi= NE\VV2MzPDJ7N{OwNC=>
RKO NIr5SnRHfW6ldHnvckBCe3OjeR?= MVmycW0> MVqxbC=> NXPsWVA3TE2VTx?= MknZ[Y5p[W6lZYOgZ4VtdCCmZXH0bEBjgSCpZXzkZY5idXmlaX6= NVzrRXZ4OjR{OUG3O|c>
HepG2 E47 M4rtTGZ2dmO2aX;uJGF{e2G7 M2fF[VIvPW2P MoT5OFhp NHn1N|FqdmO{ZXHz[ZMhfGinIITvfIlkcXS7IH;mJGFCNCCEU1:sJIFv\CCFQ3y0 NX3VNYdQOjR{N{O3N|g>
LoVo  MYDBdI9xfG:|aYOgRZN{[Xl? NXqwOGQxPW2P NH3qUYs1QGh? MmTo[Y5p[W6lZYOgSGNCNWmwZIXj[YQh[XCxcITvd4l{Ng>? NFzEb3kzPDJyMUixNi=>
WiDr NFjHU2dHfW6ldHnvckBCe3OjeR?= NYXEe4FmOTCvTR?= MoWzNYg> NUi5V|U6cW6qaXLpeJMhWEOETD3pcoR2[2WmIFzDN{BKUSCneIDy[ZN{cW:w M{O5WVI1OTlyNEi5
H1299  Ml[4SpVv[3Srb36gRZN{[Xl? NVS1U3VHOTCvTR?= NH7jZpM1cA>? M32ySYlv[3KnYYPld{BkcXOybHH0bY4ucW6mdXPl[EBk\WyuIHTlZZRp MVOyOFE4OzJyOB?=
H460 MYjGeY5kfGmxbjDBd5NigQ>? NVrGZ5pJOTCvTR?= NGDGc5k1cA>? NX\KfnFUcW6lcnXhd4V{KGOrc4DsZZRqdi2rbnT1Z4VlKGOnbHyg[IVifGh? M4rFU|I1OTd|MkC4
A549 NVrpXm5PTnWwY4Tpc44hSXO|YYm= NVrITVY4OTCvTR?= Mo\ROIg> M4fEUolvcGmkaYTzJIF2fG:yaHHnfUBqdmS3Y3XkJIJ6KGm{cnHkbYF1cW:w NYDVVIZPOjRzNEK3N|U>
Hep3B M4\iXmFxd3C2b4Ppd{BCe3OjeR?= M4G5OFVuVQ>? NF;GRnMzPGh? MoTtZZR1\W63YYTld{BVVkZvzsGgdJJwfGWldHnvckBi\2GrboP0JJNmenWvIIP0ZZJ3[XSrb36tcYVlcWG2ZXSgZZBweHSxc3nz NVH5RnJKOjRyNk[2PVM>
SMMC-7721 MonuRZBweHSxc3nzJGF{e2G7 NXrtW3IyPW2P NWq2fVZROjSq MWjheJRmdnWjdHXzJHRPTi4QsTDwdo91\WO2aX;uJIFo[Wmwc4Sgd4VzfW1ic4ThdpZifGmxbj3t[YRq[XSnZDDhdI9xfG:|aYO= NETWZ5czPDB4Nk[5Ny=>
HO8910 MlHNRZBweHSxc3nzJGF{e2G7 M3jkcVExdU1? NELVW4gyOmh? NV24ZYEy\W6qYX7j[ZMhSjF7LXnu[JVk\WRiYYDvdJRwe2l? MlnwNlM6QDN4MUC=
MCF7 NFzpRolHfW6ldHnvckBCe3OjeR?= M1u4e|VuVQ>? MlLmNlRp MXrpcoNz\WG|ZYOgR5VQKGmwZIXj[YQh[2WubDDk[YF1cA>? MljMNlM6PjJ4Mkm=
HONE-1  NIjkd49HfW6ldHnvckBCe3OjeR?= NFrJcFI2dU1? MYKxbC=> M4S2cZJmeHKnc4Pld{A3ei2vZXTpZZRm\CCUT2OgdJJw\HWldHnvci=> MUWyN|g6OjN3OB?=
HeLa MlPJSpVv[3Srb36gRZN{[Xl? NFu5WYkyOG2P NH\r[W8zcA>? NHft[VB{fXCycnXzd4V{KEyFMzDJTUBmgHC{ZYPzbZNwdg>? NWHVN495OjN6NkS3N|g>
HepG2 NUXoPFJzTnWwY4Tpc44hSXO|YYm= M3yyVFExdU1? MV[yOIg> MY\pcohq[mm2czDzbXRKT0GULTDhcoQhUEKVUz3pcoR2[2WmIHH1eI9xcGGpeR?= M3\ZS|I{QDF5MESw
SH-SY5Y M1fCdGZ2dmO2aX;uJGF{e2G7 MXqxcW0> MVeyOIg> NYG3RmpScW6qaXLpeJMhfGinIHH1eI9xcGGpeTDpcoR2[2WmIHL5JHRQS1B? NIPxU4UzOzd2M{G0PC=>
SH-SY5Y Mk\URZBweHSxc3nzJGF{e2G7 Ml:1OY1O MVSxbC=> M{XCUYFjd2yrc3jld{Bk\Wyjc4Tyc4whdmW3cn;wdo91\WO2aY\lJIVn\mWldB?= MoPsNlM3OTl|OUW=
PC12  NGPyTYlHfW6ldHnvckBCe3OjeR?= MWCxNI1O MYGyOIg> M1XVbZdifGW{ NVvZb|JnyqCrbnjpZol1eyClaIntc5RzgXC|aX6tcIls\SCycn;0[YF{d22jbDDhZ5Rqfmm2eT6= NVnMNFVyOjN4MEO5O|k>
OV2008 NUHxUFhkSXCxcITvd4l{KEG|c3H5 M1HCclVuVQ>? NYHVSJRVOjSq M1\rN4Nwdn[ncoTzJGZVYTd{MDD3bZRpKEOGRGCgbY51dyCjbjDh[IRqfGm4ZTDl[oZm[3RidH;3ZZJleyCtaXzsbY5oKG:4YYLpZY4h[2GwY3XyJINmdGy| NYDrbmZpOjN3OUKyPFE>
A2780 Mo\NRZBweHSxc3nzJGF{e2G7 MlTqOY1O NIXjR3EzPGh? M1z2XINwdn[ncoTzJGZVYTd{MDD3bZRpKEOGRGCgbY51dyCjbjDh[IRqfGm4ZTDl[oZm[3RidH;3ZZJleyCtaXzsbY5oKG:4YYLpZY4h[2GwY3XyJINmdGy| MWCyN|U6OjJ6MR?=
Saos-2  NXLxUpYxSXCxcITvd4l{KEG|c3H5 M2LoWlFuVQ>? M3r0b|k3cA>? M3LyUYlv[3KnYYPld{Bk\WyuIHTlZZRpKGmwZIXj[YQh[nliUFPY NF7PfmwzOzV4M{G3NS=>
1321N1 MYDDfZRwfG:6aXPpeJkhSXO|YYm= MUW1cW0> MVGyOIg> MVTwdo91\WO2czDj[YxtKGGpYXnud5QhWEOQLXnu[JVk\WRidH;4bYNqfHl? M{H0NlI{PTJ3Mk[1
SH-SY5Y NWXTeGVIS3m2b4TvfIlkcXS7IFHzd4F6 Mkf1OY1O Ml6wNlRp MYfpcoNz\WG|ZYOgVGNPKHSxeHnjbZR6 MXmyN|UzPTJ4NR?=
HT-29  MnPjSpVv[3Srb36gRZN{[Xl? MUGxcW0> MlHSOFgwQT[q NEnQV49qdmirYnn0d{BCVVCNIHnu[JVk\XNiYYX0c5Bp[WerYzDj[YxtKGSnYYTo M{HndlI{PTB6Mkey
OR6 M{TzeWZ2dmO2aX;uJGF{e2G7 MnrWNVBuVQ>? NVz5R5dzPzKq NGnNRW5{fXCycnXzd4V{KEiFVjDy[ZBtcWOjdHnvckBidmRiZn;ycYF1cW:wIH;mJIF2fG:yaHHnc5NwdWW| MYGyN|M6PTh5NR?=
Hela  NVnWZWl[TnWwY4Tpc44hSXO|YYm= NXjueWl7PW2P NUfMOWNzOjSq MlrNbY5pcWKrdIOgd5Rien[jdHnvck1qdmS3Y3XkJIF2fG:yaHHnfS=> MXWyN|M6PTZ5OR?=
MCF-7  NGjVcXVHfW6ldHnvckBCe3OjeR?= Mk\mOY1O M{PhbFI1cA>? MmjGbY5pcWKrdIOgd5Rien[jdHnvck1qdmS3Y3XkJIF2fG:yaHHnfS=> NYLWXHRVOjN|OUW2O|k>
HUVECs NHPPU3lHfW6ldHnvckBCe3OjeR?= M4nXdVNuVQ>? M{S4T|I1cA>? MkG2Zoxw[2u|IITo[UBxem:2ZXP0bZZmKGWoZnXjeEBw\iC{ZYP2[ZJifHKxbDDifUBqdmirYnn0bY5oKGG3dH;wbIFogQ>? MkizNlM{PTh7Mki=
T24 NF\kOZdHfW6ldHnvckBCe3OjeR?= M1LhZVExdU1? NF3YN5YycA>? NF7nVGdz\WS3Y3XzJJRp\SClbHXheoFo\SCxZjDMR|Mh[W[2ZYKgZoFq[2GuaX6geJJm[XSvZX70 NFvmd4IzOzN3NEC4NC=>
U251MG  MYjGeY5kfGmxbjDBd5NigQ>? MojwN41O MUmxbC=> NEDycpZ{fXCycnXzd4V{KEyFMz3JTUBxem:2ZXnuJIV5eHKnc4Ppc44> NXPTR25WOjN|M{i2NVg>
GTL-16  NWW1NmZMSXCxcITvd4l{KEG|c3H5 NIPXepA2dU1? MnvpNlRp NWjSeFd3emWmdXPld{Bk\WyuII\pZYJqdGm2eTDhd{Bkd22yYYLl[EB1dyClZXzsd{B1emWjdHXkJJdqfGhiTVXUJIlvcGmkaYTvdpM> MVWyN|MyOzR7MB?=
T-47D NWjxb5hvTnWwY4Tpc44hSXO|YYm= MlTzNVBuVQ>? NHnQU3EzcA>? NXnxNYFkcW6qaXLpeJMh[XW2b4DoZYd6KHC{b3Pld5Mh[W6mIHnuZ5Jm[XOnczDyZZBidXmlaX6gbY5lfWOnZDDhdI9xfG:|aYO= MkHGNlM{ODByMk[=
PaCa44 MnnNRZBweHSxc3nzJGF{e2G7 NXzYTmpwOi53bV2= M1e3SVFp NUfvVmZTemWmdXPld:Kh\2WwaYDpck1u\WSrYYTl[EBieG:ydH;zbZM> MV[yN|EzPDFzMh?=
MDA-MB231 MonZSpVv[3Srb36gRZN{[Xl? MmG4OY1O NVHl[ml4OWh? MUnpcoNz\WG|ZYOgdoV{fmW{YYTyc4wudWWmaXH0[YQh[2G|cHHz[UBi[3SrdnH0bY9vKGGwZDDj[YxtKGSnYYTo NIK4coczOzB6OEi1NC=>
HeLa NFP3TJBCeG:ydH;zbZMhSXO|YYm= M1H5RVExdU1? MXWybC=> M{DTUYRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDjc{11emWjdH3lcpQhf2m2aDDQSWk> MVSyN|AxODF|NR?=
SK-HEP-1 NGDFTFhCeG:ydH;zbZMhSXO|YYm= MXSxNI1O MXSxbC=> NXPJXJp{eHKxdHXjeJMh[WejaX7zeEBifXSxcHjh[5kh[W6mIHnu[JVk\XNiYYDvdJRwe2m|IHnuJIJ2\mGuaX6teJJm[XSnZDDj[Yxtew>? NGHhVmkzOjh3OE[0PS=>
HepG2 NIHYVpRCeG:ydH;zbZMhSXO|YYm= MlrFN41O MXm1bC=> MoDRdoVlfWOnczDj[YxtKGGyb4D0c5NqeyCrbnT1Z4VlKGK7IGHEdy=> NUnyRYNGOjJ6M{[1PVU>
MCF-7  MnG5SpVv[3Srb36gRZN{[Xl? M{nsPFJuVQ>? NXXtOm9xOjSq M3zWUIlvcGmkaYTzJIF2fG:yaHHnfUBqdmS3Y3XkJIJ6KFSPwrC= MVeyOFk4ODZ5Nh?=
MDA-MB-231 NVzvWFVxTnWwY4Tpc44hSXO|YYm= NYTuZXh4Om2P M4T4fVI1cA>? NVriU3FocW6qaXLpeJMh[XW2b4DoZYd6KGmwZIXj[YQh[nliVF5CpC=> NFTXPVQzPDl5ME[3Oi=>
MCF-7  NIjNeWJHfW6ldHnvckBCe3OjeR?= NULON|NJOm2P MWi0PIg> NUfrVFQ{eHKxbX;0[ZMhXE1vaX7keYNm\CClZXzsJIRm[XSq MnyxNlQ6PzB4N{[=
MDA-MB-231 MnjDSpVv[3Srb36gRZN{[Xl? MnOzNo1O MVm0PIg> NYqyV3Q2eHKxbX;0[ZMhXE1vaX7keYNm\CClZXzsJIRm[XSq NYDDdnd5OjR7N{C2O|Y>
PANC-1  NFTJWm9CeG:ydH;zbZMhSXO|YYm= NGHRW3gydU1? M3TROVQ5cA>? NGmyN|RFVVOR NHnPTFRmdmijbnPld{Bjd3K2ZYrvcYljNWmwZIXj[YQh[2WubDD2bYFjcWyrdImgcI9{ew>? M1;K[FI1QDR{MUW4
MDA-MB 231 MYXBdI9xfG:|aYOgRZN{[Xl? NXWyTFBOPW2P MYWwMlVp MYftc4R2dGG2ZYOgWI9kd22rbtMuxsBqdmS3Y3XkJIFxd3C2b4Ppdy=> MkPSNlQ5OzB5OEG=
Microglia  MkK3RZBweHSxc3nzJGF{e2G7 NVixVFhIPW2P MlPqNlRp NWPsZm91\GWlcnXhd4V{KGi7cH;4bYEucW6mdXPl[EBk\WyuIHTlZZRp M3v4[lI1QDF6NkCx
A2780cp MV7BdI9xfG:|aYOgRZN{[Xl? MXGyMlVuVQ>? MV6xbC=> NUm0Z2ZK\GSKMl:= MX3lcohidmOnczDjbZNxdGG2aX6tbY5lfWOnZDDj[YxtKGSnYYTo M2XK[VI1QDF5OUS2
HepG2 NGLBTHBHfW6ldHnvckBCe3OjeR?= MXe1cW0> NYTMZm1PPGh? M13aTolv[3KnYYPld{Bk\WyudXzhdkBt\X[nbIOgc4YhUE{EoB?= NH3MRWIzPDdzM{W4Oy=>
U2OS NVjzTXhKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmO5NVBuVQ>? M{\2U|I1cA>? NHfrU|lqdnSnboPp[olmeyC2aHWg[5Jwf3SqIHnubIljcXSrb36gbY5lfWOnZDDifUBFd3h? MXuyOFY{QTBzMx?=
HCT116 MWXBdI9xfG:|aYOgRZN{[Xl? MkDwOY1O NGH0PGUzPGh? NYHQXGlNTE2VTx?= MUnlcohidmOnczDhdIlo\W6rbj3pcoR2[2WmIHPlcIwh\GWjdHi= MnLLNlQ3OjZ3MkK=
MCF-7  MXvBdI9xfG:|aYOgRZN{[Xl? NFrHT5kxNjGvTR?= NIrEb5o3cA>? M1;XfIVvcGGwY3XzJJNqenSrbn;sMYlv\HWlZXSgZZBweHSxc3nz MofwNlI4PTF7OEm=
PC-3  NVH3fXg1SXCxcITvd4l{KEG|c3H5 MkHTNo1O M1HweFJp NXGzeotncW6lcnXhd4V{KE:UST3pcoR2[2WmIHPlcIwh\GWjdHi= MYeyNlc1PTV6MB?=
U251  Mk\vRZBweHSxc3nzJGF{e2G7 MXS1cW0> M4DscVI1cA>? MlfQbY5kemWjc3XzJHMyNWmwZIXj[YQh[2WubDDk[YF1cA>? NHPXZWgzOjV5OUe4PC=>
HeLa  MnHnRZBweHSxc3nzJGF{e2G7 M4PhTVVuVQ>? NEnF[mkzPGh? NGfkUnBqdmS3Y3XzJINie3Cjc3Wt[IVx\W6mZX70JINmdGxiZHXheIg> NHzGRoozOjV2NUGyPC=>
A549 NXPtRohlTnWwY4Tpc44hSXO|YYm= NHj0e4UxNjGvTR?= NVjHR3ZzOjSq Mke5d5VxeHKnc4Pld{BUXTFzMke0MYlv\HWlZXSgZ4VtdCCmZXH0bC=> MkLPNlI1PjZ7NkC=
pDCs NXHwUXUzTnWwY4Tpc44hSXO|YYm= M3fXbVExdU1? M1nGcFAvPWh? MVVCpJJm\HWlZYOgeIhmKGmwZIXjeIlwdiCxZjDJSm4u|rFiYomgd5NTVkF2MB?= Mnz3NlI{QTZ3OUm=
BGC-823 M3KyT2Z2dmO2aX;uJGF{e2G7 NUHkRmh{PW2P NVHQd|Y2Omh? NWHvZo4{cW6qaXLpeJMhfGinIILheIUhd2ZiYYX0c5Bp[WerYzDj[Yxtew>? M4C0NVIzOzJ{MUWy
U937 NV2wS25NTnWwY4Tpc44hSXO|YYm= MVqycW0> MW[xNog> MYjk[YNz\WG|ZYOgeIhmKGG3dH;wbIFogSC{YYTpc:Kh NVTscYI{OjJzNUWxOVA>
Marc-145 MoLGSpVv[3Srb36gRZN{[Xl? MYm1cW0> MUmxNk8zPC9|Nni= MVzy[YR2[2W|IITo[UBRWlKVVjD0bZRmenNiYX7kJJRp\SCycn;0[YlvKGW6cILld5Nqd25? MVWyNlEyQTlyMB?=
HBx  MVfBdI9xfG:|aYOgRZN{[Xl? M1vzN|ExdU1? M33sNFQ5cA>? NELpUZFFVVOR MV3pcoNz\WG|ZYOgZ4VtdCCmZXH0bC=> MUOyNlAzODB5OB?=
MCF-7 MVXGeY5kfGmxbjDBd5NigQ>? M2TONVExdU1? MXi0PIg> MUXicI9kc3NiYYX0c5Bp[We7IHnu[JVk\WRiYomgZo9zfGW8b33pZi=> MVyyNVk{OTl|Nx?=
RMPI8226  MULGeY5kfGmxbjDBd5NigQ>? Ml;VOY1O M4XoVFFp M3LGc5N2eHC{ZYPz[ZMhfGinIHzleoVtKG:oIHH1eI9xcGGpeTD1coRmeiCwdYTybYVvfCCmZYDs[ZRqd25? NHu3SFczOTlzNU[yNC=>
PC12/TetOn NETlWlBHfW6ldHnvckBCe3OjeR?= NX;rc4VLOC5zL{HtUS=> M1rSb|E5cA>? MkjBcIVi\HNidH:g{tEue3mwKGfULUBi[2O3bYXsZZRqd25uIITvfIlkcXS7LDDhcoQhd2yrZ3;t[ZIh\m:{bXH0bY9vyqB? MVeyNVkxPjZ3OR?=
HeLa  MWTDfZRwfG:6aXPpeJkhSXO|YYm= MVuycW0> MlLqNlRp NVvNXJZPcW6qaXLpeIV{KHSqZTDjfZRwfG:6aXPpeJkhd2Zic3nsbYJqdmmwIITvJGhmVGFiY3XscJMv MnXWNlE5PzV|OEW=
Jurkat M{LqNWZ2dmO2aX;uJGF{e2G7 NGfPbo8yOG2P NU\mOWg2OWh? M4Xa[4Rm[3KnYYPld{B1cGViZYjwdoV{e2mxbjDv[kBNSzNvSVmgZY5lKHSqZTDmc5Ju[XSrb36gc4Yh[XW2b4DoZYdwe2:vZYO= MlXQNlE5PjRyM{e=
K562 NVrTNmJFTnWwY4Tpc44hSXO|YYm= M4PaNlExdU1? MlvPNYg> NH65bFFl\WO{ZXHz[ZMhfGinIHX4dJJme3Orb36gc4YhVEN|LVnJJIFv\CC2aHWg[o9zdWG2aX;uJI9nKGG3dH;wbIFod3OxbXXz M17ze|IyQDZ2MEO3
Nara-H M3HqZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGrzS242dU1? NGDDXXI1QGh? MYflcohidmOnczD0[Y1{cXKxbHnteZNu\WSrYYTl[EB{fXCycnXzd4lwdiCxZjDOZZJiNUhiY3XscEBxem:uaX\ldoF1cW:w NFnMVGQzOThyNUCzNy=>
HUVECs MUTGeY5kfGmxbjDBd5NigQ>? NXj4PFJ3OTCvTR?= NFu2NoYxNjWq Mk\j[IVkemWjc3XzJJRp\SCDR1WtRnNCcW6mdXPl[EBifXSxcHjh[5khdGW4ZR?= MX:yNVQ3QDV7Mh?=
HepG2 MUfBdI9xfG:|aYOgRZN{[Xl? MlPkNo1O MkTxNYg> MnPI[Y5p[W6lZYOgdoFlcWG2aX;uMYlv\HWlZXSgZ4VtdCCmZXH0bC=> MV:yNVQ2OzZ7MR?=

... Click to View More Cell Line Experimental Data

In vivo 3-Methyladenine blocks autophagy through its effect on class III phosphatidylinositol 3-kinase (PI3K). 3-Methyladenine treatment does not alter the degree of hemorrhage compared with the subarachnoid hemorrhage (SAH) group. 3-Methyladenine pretreatment significantly aggravates neurological symptoms when compared with the SAH + vehicle group. Autophagy is decreased when 3-Methyladenine treatment is applied. Conversely, cleaved caspase-3 is markedly up-regulated in the SAH + 3-Methyladenine group. In line with the up-regulation of cleaved caspase-3 expression, the number of TUNEL-positive cells in the right cortex is significantly increased in the SAH + 3-Methyladenine group compared with the SAH + vehicle group. [5]

Protocol

Kinase Assay:[4]
+ Expand

Protein degradation assay:

HeLa cells are radiolabeled for 24 hours with 0.05 mCi/mL l-[U- 14C]valine. At the end of the labeling period, cells are rinsed three times with PBS. Cells are incubated for the designated times in either full medium or EBSS with or without the presence of 10 mM 3-Methyladenine.
Cell Research:[2]
+ Expand
  • Cell lines: HeLa cell line
  • Concentrations: 1-10 mM
  • Incubation Time: 24, 48 or 72 hours
  • Method: Cell (such as HeLa cell) viability is determined by a trypan blue exclusion assay. Briefly, after treated with 3-Methyladenine, both adherent and floating cells are collected and suspended in phosphate buffered saline (PBS, pH 7.4) at a final density of 1-2 × 106/mL. An equal volume of 0.4% trypan blue solution (w/v, in PBS) is added to the cell suspension and mixed thoroughly. After incubation at room temperature for 3 min, cell counting is performed using a hemacytometer.
    (Only for Reference)
Animal Research:[5]
+ Expand
  • Animal Models: Adult male Sprague–Dawley rats weighing 300-350 g
  • Formulation: Saline
  • Dosages: 400 nM
  • Administration: Intracerebral ventricular
    (Only for Reference)

Solubility (25°C)

In vitro Water 10 mg/mL warmed (67.04 mM)
Ethanol 4 mg/mL (26.81 mM)
DMSO 3 mg/mL warmed (20.11 mM)
In vivo Add solvents to the product individually and in order:
water
For best results, use promptly after mixing.
10mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 149.15
Formula

C6H7N5

CAS No. 5142-23-4
Storage powder
Synonyms NSC 66389

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
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Molecular Weight Calculator

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Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    I'm also wondering whether it can be dissolved in water,or maybe something like culture medium,normal saline solution to form 10mM solution

  • Answer:

    As the reference (http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal. pone.0035665), 3-MA, which was found to inhibit autophagy at concentrations ranging from 1 to 10 mM was directly dissolved into the culture medium at the indicated concentrations. And we tested the solubility of S2767, and found the solubility of 3-MA in DMEM is 31 mg/mL at about 40°C.

PI3K Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID