3-Methyladenine (3-MA)

Catalog No.S2767 Synonyms: NSC 66389

3-Methyladenine (3-MA) Chemical Structure

Molecular Weight(MW): 149.15

3-Methyladenine (3-MA) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation.

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4 Customer Reviews

  • MDC-labeled vacuoles were induced by AZD2014 and inhibited by autophagy inhibitor (3-MA). SMMC-7721 cells were treated with AZD2014 or rapamycin at concentrations of 100 and 600 nM, respectively, for 48 hours in the presence or absence of 3-MA, and then stained with MDC. Cells were immediately observed under a confocal microscope. Cells in the control group were treated with DMSO. bars, 20 μm.

    Am J Cancer Res, 2015, 5(1): 125-139. 3-Methyladenine (3-MA) purchased from Selleck.

    Cells were treated with DMSO, TDCIPP (100 μM) only, or pretreated with 3-MA before treatment with TDCIPP (100 μM). LC3 conversion and beclin-1 and p62 expression were assessed by western blotting at 24 h

    Food Chem Toxicol, 2017, 100:183-196. 3-Methyladenine (3-MA) purchased from Selleck.

  • PC-9/ER cells were treated with CX-4945 (5 mM) for 48 h in the presence or absence of 3-MA (2 mM) and Atg7 siRNA (100 nM). Cleavage of PARP-1 and caspase-3 was shown by Western blot analysis. CT: without CX-4945; CX: CX-4945; CX+G: CX-4945 + gefitinib; CX+E: CX-4945 + erlotinib.

    PLoS One, 2014, 9(12): e114000 . 3-Methyladenine (3-MA) purchased from Selleck.

    A: The survival ratio of AML12 cells was measured after treatment with rapamycin or 3-MA; B: Western blot was used to detect the expression of LC3 and P62 in AML12 cells treated with Rapamycin or 3-MA.

    World J Gastroenterol, 2016, 22(18): 4501-4514.. 3-Methyladenine (3-MA) purchased from Selleck.

Purity & Quality Control

Choose Selective PI3K Inhibitors

Biological Activity

Description 3-Methyladenine (3-MA) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation.
Targets
Vps34 [1]
(HeLa cells)
PI3Kγ [1]
(HeLa cells)
25 μM 60 μM
In vitro

The slight preference for Vps34 prevention by 3-Methyladenine probably arises from a hydrophobic ring specific to Vps34, which encircles the 3-methyl group of 3-Methyladenine. [1] 3-Methyladenine has been reported to cause cancer cell death under both normal and starvation conditions. 3-Methyladenine could also suppress cell migration and invasion independently of its ability to inhibit autophagy, implying that 3-Methyladenine possesses functions other than autophagy suppression. 3-Methyladenine elicits caspase-dependent cell death that is independent of autophagy inhibition. Treatment with 5 mM 3-Methyladenine reduces the percentage of glucose-starved HeLa cells displaying GFP-LC3 puncta to 23%. The levels of LC3-I are increasing and the levels of LC3-II are decreasing between 12 and 48 hours in cells that are treated with 3-Methyladenine. Conversion of LC3-I to LC3-II is suppressed by 3-Methyladenine. Treatment of HeLa cells with 3-Methyladenine at 2.5 mM or 5 mM for one day does not affect cell viability, whereas treatment with 10 mM 3-Methyladenine for one day causes a 25.0% decrease in cell viability. Treatment of cells with 2.5, 5 or 10 mM 3-Methyladenine for two days causes 11.5%, 38.0% and 79.4% decrease in viability, respectively. 3-Methyladenine decreases cell viability in a time- and dose-dependent manner. 3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest. [2] Suppression of autophagy by 3-Methyladenine inhibits SU11274-induced cell death. [3] Prolonged treatment with 3-Methyladenine (up to 9 hours) induces significant LC3 I to II conversion in wild type MEFs. Prolonged treatment with 3-Methyladenine, but not wortmannin, markedly increases GFP-LC3 punctuation/aggregation. 3-Methyladenine-induced LC3 conversion and free GFP liberation are ATG7-dependent. 3-Methyladenine treatment leads to evident increase of p62 protein level. 3-Methyladenine increases the p62 level even in Atg5−/− MEFs as well as in cells with DOX-mediated deletion of ATG5. 3-Methyladenine inhibits class I and class III PI3K in different temporal patterns. 3-Methyladenine-induced LC3 I to LC3 II conversion is dramatically compromised in Tsc2−/− cells compared with wild type cells.3-Methyladenine disrupts the anti-autophagic function of mTOR complex 1. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
95D MlfDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1KxUVNuVQ>? MYmybC=> MVXEUXNQ M{TXbpJm\HWlZYOg[5Jwf3SqIHnubIljcXSxcomg[YZn\WO2IH;mJGJFVUN? NIrxXYEzPTdzNkW2NS=>
A549  NUXCXmh3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NInYXoY{dU1? NGTNNo4zcA>? NVjZcVJnTE2VTx?= MYHy[YR2[2W|IHfyc5d1cCCrbnjpZol1d3K7IHXm[oVkfCCxZjDCSG1E M1jwdVI2PzF4NU[x
95D NXfUOJF5SXCxcITvd4l{KEG|c3H5 MnzCOY1O MWqybC=> NVTIbIVoTE2VTx?= NHL2XW1qdmirYnn0d{BDTE2FLXnu[JVk\WRiYYDvdJRwfGmlIHPlcIwh\GWjdHi= MoHrNlU4OTZ3NkG=
A549  MWPBdI9xfG:|aYOgRZN{[Xl? MnjVOY1O NXGySHB[Omh? M1vVO2ROW09? MnywbY5pcWKrdIOgRmROSy2rbnT1Z4VlKGGyb4D0c5Rq[yClZXzsJIRm[XSq NGnGSJozPTdzNkW2NS=>
NBL-W-S  M2KwZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVuxcW0> MmiwOog> NYfYU3Fw\W6qYX7j[ZMhT0GQVD22NUB1d3irY3n0fS=> NEfUUGMzPTN{M{KyNi=>
NBL-W-S  Mn\TRZBweHSxc3nzJGF{e2G7 Mo\ZNY1O MnvGOog> NUfQO4Y6cW6lcnXhd4V{KGOnbHygZZBweHSxc3nzJIlv\HWlZXSgZpkhT0GQVD22NS=> NULVTI9JOjV|MkOyNlI>
A2780cp  M33kc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXrCWZBJOW2P NGe0W3YycA>? NYTYWW9ZcW6lcnXhd4V{KGOrc4DsZZRqdi2rbnT1Z4VlKGOnbHyg[IVifGh? NYfnRlZJOjV|MkK2PVQ>
A549  M4fGRmZ2dmO2aX;uJGF{e2G7 MofBOY1O NI\X[3MzcA>? M2DpPZN2eHC{ZYPz[ZMhW1CFIHnu[JVk\WRiYXPjeY12dGG2aX;uJI9nKEyFMz3JTS=> MnzWNlUzQDV4Mki=
H157 Mn7zSpVv[3Srb36gRZN{[Xl? M4jSXlVuVQ>? NF;wcokzcA>? NIfsWXp{fXCycnXzd4V{KFOSQzDpcoR2[2WmIHHjZ5VufWyjdHnvckBw\iCOQ{OtTWk> M{\PfFI2Ojh3NkK4
MDR MYHBdI9xfG:|aYOgRZN{[Xl? NYTMTlRTOTCvTR?= M1;vRVZp MXTzeJJmdme2aHXud{B1cGVicH;3[ZIhd2ZiYX70bYNidmOncjDkdpVoew>? NYmxNWlDOjVyMUm3NFE>
HT-29  NIm3UIJCeG:ydH;zbZMhSXO|YYm= NH;zZpkydU1? M1Xu[|Q5cA>? M1HlT2ROW09? Mn;p[Y5p[W6lZYOgZo9zfGW8b33pZk1qdmS3Y3XkJINmdGxidnnhZoltcXS7IHzvd5M> NUXCXYpjOjR6NEKxOVg>
Microglia  NIfxc45CeG:ydH;zbZMhSXO|YYm= M4nKVVVuVQ>? MX[yOIg> NFnpcI1l\WO{ZXHz[ZMhcHmyb4jpZU1qdmS3Y3XkJINmdGxiZHXheIg> MUmyOFgyQDZyMR?=
A2780cp M4\mUWFxd3C2b4Ppd{BCe3OjeR?= M33sdFIvPW2P MYexbC=> Mnj1[IRJOk9? M4\aVIVvcGGwY3XzJINqe3CuYYTpck1qdmS3Y3XkJINmdGxiZHXheIg> MVuyOFgyPzl2Nh?=
HepG2 NF72PY1HfW6ldHnvckBCe3OjeR?= MUK1cW0> MXi0bC=> Mly4bY5kemWjc3XzJGhNKHKnbHXhd4U> MUeyOFcyOzV6Nx?=
A549 NW\2b|RqSXCxcITvd4l{KEG|c3H5 M3zWbFVuVQ>? M1vzZVQ5cA>? M2TTeoRm[3KnYYPld{B1cGVicHXyZ4VvfGGpZTDv[kBk\WyuIHTlZZRpKGGwZDDlfJBz\XO|aX;uJIxmfmWuczDv[kBk[XOyYYPlMVMtKEKnY3zpck0yKGGwZDDMR|MuUUl? NIjkeFEzPDdyNkOwNy=>
U2OS NF7rb|NCeG:ydH;zbZMhSXO|YYm= Mn;BNVBuVQ>? M4W3TVI1cA>? NHO0eGlqdnSnboPp[olmeyC2aHWg[5Jwf3SqIHnubIljcXSrb36gc4YhfGinIGWyU3Mh[2WubIOgbY5lfWOnZDDifUBFd3h? NVXUNHF3OjR4M{mwNVM>
Saos-2 NYrFc3RSSXCxcITvd4l{KEG|c3H5 M{TDV|ExdU1? NUTKNFdoOjSq NFjKW|FqdnSnboPp[olmeyC2aHWg[5Jwf3SqIHnubIljcXSrb36gc4YhfGinIGWyU3Mh[2WubIOgbY5lfWOnZDDifUBFd3h? MVOyOFY{QTBzMx?=
A549 M{HSdmFxd3C2b4Ppd{BCe3OjeR?= MVGxNI1O MVO0PIg> NVK1XVFS[WOlZXzldoF1\XNidHjlJJJm\HWldHnvckBw\iClZXzsJJZq[WKrbHn0fUBqdmS3Y3XkJIJ6KFCWWB?= NVvadZpSOjR4Mk[3NlI>
HCT116  NIL6cW9CeG:ydH;zbZMhSXO|YYm= M3vafFVuVQ>? MkP5NlRp M4\oOYVvcGGwY3XzJJRp\SCjcH;weI9{cXNiaX7keYNm\CCkeTDhdIlo\W6rbh?= NXPTPXNvOjR4Mk[1NlI>
HGC-27 MojDSpVv[3Srb36gRZN{[Xl? NUHoTmxLOTCvTR?= M2OzclFp MYDpcohq[mm2czD0bIUh[2WubDD2bYFjcWyrdImgcI9{eyCkeTDSRWQxODFib4KgUWsuOjJyNh?= NH63SGkzPDRzNkO0PS=>
U2OS  MVXGeY5kfGmxbjDBd5NigQ>? MnPsNE42NzGvTR?= M{naXlQ5cA>? M2H4[olv\HWlZYOgd4FtcW6xbYnjbY4ucW6mdXPl[EBk\WyuII\pZYJqdGm2eTDsc5N{ MnHxNlQ{PTh|NEK=
MG-63 MXrGeY5kfGmxbjDBd5NigQ>? MmfXNE42NzGvTR?= MWO0PIg> MYXpcoR2[2W|IIPhcIlvd227Y3nuMYlv\HWlZXSgZ4VtdCC4aXHibYxqfHlibH;zdy=> MWWyOFM2QDN2Mh?=
MG-63 MnfGRZBweHSxc3nzJGF{e2G7 MnGxNE42NzGvTR?= MnjnN|Jp MkPp[Y5p[W6lZYOgd4FtcW6xbYnjbY4ucW6mdXPl[EBk\WyuIHHwc5B1d3Orcx?= NIX3[VIzPDN3OEO0Ni=>
MG-63 NEfvUphCeG:ydH;zbZMhSXO|YYm= MVqxNI1O MX:xNog> M1KxUYVvcGGwY3XzJGRRNWmwZIXj[YQh[XCxcITvd4l{ M4LEdVI1OzV6M{Cx
NTUB1 NUOzNplnSXCxcITvd4l{KEG|c3H5 M2DkRVVuVQ>? Ml60NU42cA>? MmDXdI91\W62aXH0[ZMh[2WuZXPvfIljNWmwZIXj[YQh[XCxcITvd4l{ NETVfW4zPDN2OUG3Oi=>
T24 M4HZdWFxd3C2b4Ppd{BCe3OjeR?= MojDOY1O M4rVTFEvPWh? M{jOVZBwfGWwdHnheIV{KGOnbHXjc5hq[i2rbnT1Z4VlKGGyb4D0c5Nqew>? NEjSNJUzPDN2OUG3Oi=>
SGC-7901  NHTrbppCeG:ydH;zbZMhSXO|YYm= NUXKc2xNOm2P M1;TVFFp NHuwVoFqdmO{ZXHz[ZMhS0FvNDDpcoR2[2WmIHHwc5B1d3Orcx?= M1zhWFI1OzJzM{Sw
SMMC-7721 MV7BdI9xfG:|aYOgRZN{[Xl? MYWycW0> NHLQVZIycA>? MYnpcoNz\WG|ZYOgR2EuPCCrbnT1Z4VlKGGyb4D0c5Nqew>? NVHYVW12OjR|MkGzOFA>
ECSCs  NFj1bXNCeG:ydH;zbZMhSXO|YYm= NUXQOYlzOTCvTR?= M1fWcVRp Mlza[IVkemWjc3XzJJJieGGveXPpck11emWjdHXkJIFxd3C2b4Ppdy=> NIjqeHIzPDNzOUGwPS=>
MCF-7  Mn20SpVv[3Srb36gRZN{[Xl? MlXqNVBuVQ>? M{W2WVI1cA>? Mk\wbY5pcWKrdIOgeIhmKGG3dH;wbIFogSCrbnT1Z4VlKGK7IHPo[Y1wfGincnHwfUBlenWpcx?= M{XP[|I1OzF3NUe4
UOK257 MmHZRZBweHSxc3nzJGF{e2G7 NITSOlQ2dU1? NFXpOJg{cA>? MUflcohidmOnczDwZYNtcXSjeHXsMY1m\GmjdHXkJIFxd3C2b4Ppd:Kh M4PoOFI1OzB3NkC0
ACHN-5968 M1LudWFxd3C2b4Ppd{BCe3OjeR?= NIWzWZc2dU1? Mn;QN4g> Mlju[Y5p[W6lZYOgdIFkdGm2YYjlcE1u\WSrYYTl[EBieG:ydH;zbZPDqA>? MnfHNlQ{ODV4MES=
Huh7 NFLnO4lCeG:ydH;zbZMhSXO|YYm= Mn;0No1O M1jtb|EzcA>? MY\EUXNQ MYDpcohq[mm2czDBXmQ5ODV3LXnu[JVk\WRiY3XscEBl\WG2aB?= NHi0PVUzPDJ7N{OwNC=>
Hep3B NFHjSHRCeG:ydH;zbZMhSXO|YYm= Mmj2No1O M4\xOVEzcA>? MoXGSG1UVw>? MnHPbY5pcWKrdIOgRXpFQDB3NT3pcoR2[2WmIHPlcIwh\GWjdHi= M2nSdlI1Ojl5M{Cw
RKO NWPOfoxzTnWwY4Tpc44hSXO|YYm= MnHTNo1O M2\qdFFp MmSxSG1UVw>? MUnlcohidmOnczDj[YxtKGSnYYToJIJ6KGenbHThcoFugWOrbh?= MlfoNlQzQTF5N{e=
HepG2 E47 MVvGeY5kfGmxbjDBd5NigQ>? MYWyMlVuVQ>? MkHoOFhp M1vVTIlv[3KnYYPld{B1cGVidH;4bYNqfHlib3[gRWEtKEKVTzygZY5lKEOFbES= NYrSdoFnOjR{N{O3N|g>
LoVo  M2XTd2Fxd3C2b4Ppd{BCe3OjeR?= MVe1cW0> MnXrOFhp NXTJTZJF\W6qYX7j[ZMhTEODLXnu[JVk\WRiYYDvdJRwe2m|Lh?= MXmyOFIxOThzMh?=
WiDr MVTGeY5kfGmxbjDBd5NigQ>? NYLWb|dSOTCvTR?= NELydG0ycA>? MoWwbY5pcWKrdIOgVGNDVC2rbnT1Z4VlKEyFMzDJTUBmgHC{ZYPzbY9v MUWyOFE6ODR6OR?=
H1299  MonqSpVv[3Srb36gRZN{[Xl? NFroV4kyOG2P MXi0bC=> NFT1dJVqdmO{ZXHz[ZMh[2m|cHzheIlvNWmwZIXj[YQh[2WubDDk[YF1cA>? M2XOdlI1OTd|MkC4
H460 M2PRc2Z2dmO2aX;uJGF{e2G7 NILFPVgyOG2P NV3QeoRKPGh? MmPXbY5kemWjc3XzJINqe3CuYYTpck1qdmS3Y3XkJINmdGxiZHXheIg> MkGzNlQyPzN{MEi=
A549 NFWwbJFHfW6ldHnvckBCe3OjeR?= M2W1[|ExdU1? MoXCOIg> M1fpXYlvcGmkaYTzJIF2fG:yaHHnfUBqdmS3Y3XkJIJ6KGm{cnHkbYF1cW:w MUKyOFE1Ojd|NR?=
Hep3B M17yPGFxd3C2b4Ppd{BCe3OjeR?= M{exblVuVQ>? NYTGPWpZOjSq MoTvZZR1\W63YYTld{BVVkZvzsGgdJJwfGWldHnvckBi\2GrboP0JJNmenWvIIP0ZZJ3[XSrb36tcYVlcWG2ZXSgZZBweHSxc3nz NGj4TWwzPDB4Nk[5Ny=>
SMMC-7721 MnfHRZBweHSxc3nzJGF{e2G7 NWDNVHVFPW2P MnS2NlRp NIe1Um9ifHSnboXheIV{KFSQRj5OtUBxem:2ZXP0bY9vKGGpYXnud5Qhe2W{dX2gd5Rien[jdHnvck1u\WSrYYTl[EBieG:ydH;zbZM> MmPYNlQxPjZ4OUO=
HO8910 MnOwRZBweHSxc3nzJGF{e2G7 M{HMT|ExdU1? MYixNog> MUDlcohidmOnczDCNVkucW6mdXPl[EBieG:ydH;zbS=> MoP2NlM6QDN4MUC=
MCF7 M1TuNGZ2dmO2aX;uJGF{e2G7 NHPXUFk2dU1? M1G3bFI1cA>? NILKVGNqdmO{ZXHz[ZMhS3WRIHnu[JVk\WRiY3XscEBl\WG2aB?= MVmyN|k3OjZ{OR?=
HONE-1  MkDpSpVv[3Srb36gRZN{[Xl? MWi1cW0> MnfSNYg> NFrhSpdz\XC{ZYPz[ZMhPnJvbXXkbYF1\WRiUl;TJJBzd2S3Y4Tpc44> NHfrVIkzOzh7MkO1PC=>
HeLa MX;GeY5kfGmxbjDBd5NigQ>? MXOxNI1O M1XwWFJp MlPod5VxeHKnc4Pld{BNSzNiSVmg[ZhxemW|c3nzc44> NEjQZYkzOzh4NEezPC=>
HepG2 NY\yXlFxTnWwY4Tpc44hSXO|YYm= NHPkUG8yOG2P NF;WRWczPGh? NUTEfYVYcW6qaXLpeJMhe2mWSVfBVk0h[W6mIFjCV3MucW6mdXPl[EBifXSxcHjh[5k> MmLyNlM5OTdyNEC=
SH-SY5Y MlzzSpVv[3Srb36gRZN{[Xl? MX:xcW0> M4PrfFI1cA>? M{nWUolvcGmkaYTzJJRp\SCjdYTvdIhi\3liaX7keYNm\CCkeTDUU2NR MYmyN|c1OzF2OB?=
SH-SY5Y NUjVcpZrSXCxcITvd4l{KEG|c3H5 NFX3NnA2dU1? NXzYPYlwOWh? NEL5VY9i[m:uaYPo[ZMh[2WuYYP0do9tKG6ndYLvdJJwfGWldHn2[UBm\m[nY4S= MXeyN|YyQTN7NR?=
PC12  M3LhSWZ2dmO2aX;uJGF{e2G7 NX3GeYllOTCvTR?= NHj0eHczPGh? MlLne4F1\XJ? NXnoNFl5yqCrbnjpZol1eyClaIntc5RzgXC|aX6tcIls\SCycn;0[YF{d22jbDDhZ5Rqfmm2eT6= NX7sSVVTOjN4MEO5O|k>
OV2008 NV;lSVNwSXCxcITvd4l{KEG|c3H5 M2Dw[|VuVQ>? MXOyOIg> NVqyOHZP[2:wdnXyeJMhTlS\N{KwJJdqfGhiQ1TEVEBqdnSxIHHuJIFl\Gm2aY\lJIVn\mWldDD0c5diemS|IHvpcIxqdmdib4\hdolidiClYX7j[ZIh[2WubIO= NHThbVczOzV7MkK4NS=>
A2780 M2LUemFxd3C2b4Ppd{BCe3OjeR?= M2PHOlVuVQ>? Mn6yNlRp M2DzSYNwdn[ncoTzJGZVYTd{MDD3bZRpKEOGRGCgbY51dyCjbjDh[IRqfGm4ZTDl[oZm[3RidH;3ZZJleyCtaXzsbY5oKG:4YYLpZY4h[2GwY3XyJINmdGy| MkLyNlM2QTJ{OEG=
Saos-2  MUTBdI9xfG:|aYOgRZN{[Xl? NYjZZVdiOW2P MVm5Oog> MX7pcoNz\WG|ZYOgZ4VtdCCmZXH0bEBqdmS3Y3XkJIJ6KFCFWB?= M3[wfVI{PTZ|MUex
1321N1 MYjDfZRwfG:6aXPpeJkhSXO|YYm= NHjBXHc2dU1? MUSyOIg> Mlf6dJJwfGWldIOgZ4VtdCCjZ3HpcpN1KFCFTj3pcoR2[2WmIITvfIlkcXS7 NF;NWokzOzV{NUK2OS=>
SH-SY5Y NIj4OJVEgXSxdH;4bYNqfHliQYPzZZk> MX[1cW0> MoHyNlRp M1f1Rolv[3KnYYPld{BRS05idH;4bYNqfHl? NYq3SWtUOjN3MkWyOlU>
HT-29  NEXzZo1HfW6ldHnvckBCe3OjeR?= NVnlW2pwOW2P M1:3SlQ5Nzl4aB?= M1;GdolvcGmkaYTzJGFOWEtiaX7keYNmeyCjdYTvdIhi\2mlIHPlcIwh\GWjdHi= MXqyN|UxQDJ5Mh?=
OR6 M{jIeGZ2dmO2aX;uJGF{e2G7 NXnINIg5OTCvTR?= MXG3Nog> NIfkNph{fXCycnXzd4V{KEiFVjDy[ZBtcWOjdHnvckBidmRiZn;ycYF1cW:wIH;mJIF2fG:yaHHnc5NwdWW| NW[5cHlqOjN|OUW4O|U>
Hela  NEPMXolHfW6ldHnvckBCe3OjeR?= MVm1cW0> NUnqe|E6OjSq M1\pPYlvcGmkaYTzJJN1[XK4YYTpc44ucW6mdXPl[EBifXSxcHjh[5k> MVmyN|M6PTZ5OR?=
MCF-7  M{P0WmZ2dmO2aX;uJGF{e2G7 NGnwSWY2dU1? M1XxN|I1cA>? NYfZSItScW6qaXLpeJMhe3Sjco\heIlwdi2rbnT1Z4VlKGG3dH;wbIFogQ>? MWmyN|M6PTZ5OR?=
HUVECs NFPJSolHfW6ldHnvckBCe3OjeR?= NIDydno{dU1? NWnCbWc6OjSq M3fSR4Jtd2OtczD0bIUheHKxdHXjeIl3\SCnZn\lZ5Qhd2ZicnXzeoVz[XS{b3ygZpkhcW6qaXLpeIlv\yCjdYTvdIhi\3l? NH\rVY0zOzN3OEmyPC=>
T24 MmjLSpVv[3Srb36gRZN{[Xl? Mkm4NVBuVQ>? M{DlN|Fp MlHjdoVlfWOnczD0bIUh[2ynYY\h[4Uhd2ZiTFOzJIFnfGW{IHLhbYNidGmwIITy[YF1dWWwdB?= M4LXZVI{OzV2MEiw
U251MG  MkHtSpVv[3Srb36gRZN{[Xl? MVSzcW0> M3[zPFFp MUTzeZBxemW|c3XzJGxEOy2LSTDwdo91\WmwIHX4dJJme3Orb36= NIXTfYwzOzN|OE[xPC=>
GTL-16  MkLiRZBweHSxc3nzJGF{e2G7 M2Tq[VVuVQ>? MknzNlRp M2XaZ5Jm\HWlZYOgZ4VtdCC4aXHibYxqfHliYYOgZ49ueGG{ZXSgeI8h[2WubIOgeJJm[XSnZDD3bZRpKE2HVDDpcohq[mm2b4Lz NX3MeXlkOjN|MUO0PVA>
T-47D MoTuSpVv[3Srb36gRZN{[Xl? MmPJNVBuVQ>? MYOybC=> M2XGbolvcGmkaYTzJIF2fG:yaHHnfUBxem:lZYPzJIFv\CCrbnPy[YF{\XNicnHwZY16[2mwIHnu[JVk\WRiYYDvdJRwe2m| MlXnNlM{ODByMk[=
PaCa44 MVTBdI9xfG:|aYOgRZN{[Xl? M{jPT|IvPW2P MnXpNYg> MoDadoVlfWOnc9Mg[4VvcXCrbj3t[YRq[XSnZDDhdI9xfG:|aYO= MV:yN|EzPDFzMh?=
MDA-MB231 MVzGeY5kfGmxbjDBd5NigQ>? NG\qdnE2dU1? NGjKSZUycA>? NITKOmdqdmO{ZXHz[ZMhemW|dnXyZZRzd2xvbXXkbYF1\WRiY3HzdIF{\SCjY4TpeoF1cW:wIHHu[EBk\WyuIHTlZZRp M4PBZ|I{ODh6OEWw
HeLa NXXDV41ySXCxcITvd4l{KEG|c3H5 NWTpR4Y{OTCvTR?= NFPvblYzcA>? M17RW4Rm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDjc{11emWjdH3lcpQhf2m2aDDQSWk> NFe5Z3MzOzByMEGzOS=>
SK-HEP-1 MnfSRZBweHSxc3nzJGF{e2G7 MoCxNVBuVQ>? NIH0VmgycA>? NW\QcZdyeHKxdHXjeJMh[WejaX7zeEBifXSxcHjh[5kh[W6mIHnu[JVk\XNiYYDvdJRwe2m|IHnuJIJ2\mGuaX6teJJm[XSnZDDj[Yxtew>? NILCOIszOjh3OE[0PS=>
HepG2 NGfCT2VCeG:ydH;zbZMhSXO|YYm= MoPUN41O M13Od|Vp M{jheJJm\HWlZYOgZ4VtdCCjcH;weI9{cXNiaX7keYNm\CCkeTDRSJM> NY[x[W1POjJ6M{[1PVU>
MCF-7  MlXsSpVv[3Srb36gRZN{[Xl? MX6ycW0> Mk\hNlRp MkHubY5pcWKrdIOgZZV1d3CqYXf5JIlv\HWlZXSgZpkhXE4EoB?= M2LX[lI1QTdyNke2
MDA-MB-231 M3rIfWZ2dmO2aX;uJGF{e2G7 NHXvO48zdU1? NXL4U2JsOjSq M4[wPIlvcGmkaYTzJIF2fG:yaHHnfUBqdmS3Y3XkJIJ6KFSPwrC= NYXNXIpSOjR7N{C2O|Y>
MCF-7  NHPNXolHfW6ldHnvckBCe3OjeR?= M2XISFJuVQ>? M1fqO|Q5cA>? MXTwdo9ud3SnczDUUU1qdmS3Y3XkJINmdGxiZHXheIg> MWCyOFk4ODZ5Nh?=
MDA-MB-231 MmjWSpVv[3Srb36gRZN{[Xl? M1zER|JuVQ>? M4THNFQ5cA>? MUnwdo9ud3SnczDUUU1qdmS3Y3XkJINmdGxiZHXheIg> MV2yOFk4ODZ5Nh?=
PANC-1  NFvUd2hCeG:ydH;zbZMhSXO|YYm= MVuxcW0> NH3sblE1QGh? MV3EUXNQ NV3qdnA4\W6qYX7j[ZMh[m:{dHX6c41q[i2rbnT1Z4VlKGOnbHygeoli[mmuaYT5JIxwe3N? M2ju[lI1QDR{MUW4
MDA-MB 231 NYLLR296SXCxcITvd4l{KEG|c3H5 MnfuOY1O MmDxNE42cA>? MUftc4R2dGG2ZYOgWI9kd22rbtMuxsBqdmS3Y3XkJIFxd3C2b4Ppdy=> MYKyOFg{ODd6MR?=
Microglia  M{i5UmFxd3C2b4Ppd{BCe3OjeR?= MXq1cW0> NI\wV5EzPGh? Mljq[IVkemWjc3XzJIh6eG:6aXGtbY5lfWOnZDDj[YxtKGSnYYTo MlT2NlQ5OTh4MEG=
A2780cp MonoRZBweHSxc3nzJGF{e2G7 MmTnNk42dU1? MX2xbC=> M{nyW4RlUDKR M2ntfoVvcGGwY3XzJINqe3CuYYTpck1qdmS3Y3XkJINmdGxiZHXheIg> NWnuO2tYOjR6MUe5OFY>
HepG2 MXLGeY5kfGmxbjDBd5NigQ>? Mn[xOY1O MnjWOIg> MmT0bY5kemWjc3XzJINmdGy3bHHyJIxmfmWuczDv[kBJVMLi NEDjOGIzPDdzM{W4Oy=>
U2OS MlLTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHixVXkyOG2P M1\jVFI1cA>? MWXpcpRmdnOrZnnld{B1cGViZ4Lve5RpKGmwaHnibZRqd25iaX7keYNm\CCkeTDEc5g> NWrhbmRZOjR4M{mwNVM>
HCT116 M{GxNmFxd3C2b4Ppd{BCe3OjeR?= MX21cW0> M1z2VlI1cA>? NVzTb2NITE2VTx?= NEn0[pdmdmijbnPld{BieGmpZX7pck1qdmS3Y3XkJINmdGxiZHXheIg> MnfhNlQ3OjZ3MkK=
MCF-7  Mme5RZBweHSxc3nzJGF{e2G7 M1S2UFAvOW2P M2TBT|Zp NVm5bpVG\W6qYX7j[ZMhe2m{dHnuc4wucW6mdXPl[EBieG:ydH;zbZM> M1PQe|IzPzVzOUi5
PC-3  MUHBdI9xfG:|aYOgRZN{[Xl? M3fJdFJuVQ>? MXSybC=> MVjpcoNz\WG|ZYOgU3JKNWmwZIXj[YQh[2WubDDk[YF1cA>? M3W5VlIzPzR3NUiw
U251  MmXCRZBweHSxc3nzJGF{e2G7 NULJOJVTPW2P MX2yOIg> NGT2T5hqdmO{ZXHz[ZMhWzFvaX7keYNm\CClZXzsJIRm[XSq NYTCXYllOjJ3N{m3PFg>
HeLa  MmrDRZBweHSxc3nzJGF{e2G7 NGDyPJQ2dU1? NYj4eI1GOjSq NHfEc2hqdmS3Y3XzJINie3Cjc3Wt[IVx\W6mZX70JINmdGxiZHXheIg> NXPs[WdqOjJ3NEWxNlg>
A549 MYfGeY5kfGmxbjDBd5NigQ>? MlLzNE4ydU1? M3PtZlI1cA>? Mln3d5VxeHKnc4Pld{BUXTFzMke0MYlv\HWlZXSgZ4VtdCCmZXH0bC=> MnrMNlI1PjZ7NkC=
pDCs MnT5SpVv[3Srb36gRZN{[Xl? NUPLdWpzOTCvTR?= NWDZUGJoOC53aB?= NH\sSZLDqHKnZIXj[ZMhfGinIHnu[JVkfGmxbjDv[kBKTk5vzsGgZpkhe3OUTlG0NC=> M1qwe|IzOzl4NUm5
BGC-823 M2rHNmZ2dmO2aX;uJGF{e2G7 MX[1cW0> MonUNog> MXLpcohq[mm2czD0bIUhemG2ZTDv[kBifXSxcHjh[4lkKGOnbHzz MXOyNlMzOjF3Mh?=
U937 Ml3ESpVv[3Srb36gRZN{[Xl? NELFfHQzdU1? MYGxNog> MlPY[IVkemWjc3XzJJRp\SCjdYTvdIhi\3licnH0bY/DqA>? M4XPWlIzOTV3MUWw
Marc-145 M4riPWZ2dmO2aX;uJGF{e2G7 NI[4Z482dU1? M4TRdlEzNzJ2L{O2bC=> MlfLdoVlfWOnczD0bIUhWFKUU2[geIl1\XK|IHHu[EB1cGVicILveIVqdiCneIDy[ZN{cW:w NGS3PGMzOjFzOUmwNC=>
HBx  M3XxOWFxd3C2b4Ppd{BCe3OjeR?= MY[xNI1O M3zkSlQ5cA>? MkHsSG1UVw>? NH7VeIRqdmO{ZXHz[ZMh[2WubDDk[YF1cA>? MmP6NlIxOjByN{i=
MCF-7 MnXPSpVv[3Srb36gRZN{[Xl? MXmxNI1O MW[0PIg> MXficI9kc3NiYYX0c5Bp[We7IHnu[JVk\WRiYomgZo9zfGW8b33pZi=> MofjNlE6OzF7M{e=
RMPI8226  M3KzbmZ2dmO2aX;uJGF{e2G7 MUi1cW0> MUKxbC=> M13aW5N2eHC{ZYPz[ZMhfGinIHzleoVtKG:oIHH1eI9xcGGpeTD1coRmeiCwdYTybYVvfCCmZYDs[ZRqd25? NWC2UI5qOjF7MUW2NlA>
PC12/TetOn Mm\iSpVv[3Srb36gRZN{[Xl? M3qwW|AvOS9zbV2= M3r6VlE5cA>? MX\s[YFleyC2bzFOtU1{gW5qV2SpJIFk[3WvdXzheIlwdixidH;4bYNqfHluIHHu[EBwdGmpb33ldkBnd3KvYYTpc47DqA>? MoXjNlE6ODZ4NUm=
HeLa  M{TZcmN6fG:2b4jpZ4l1gSCDc4PhfS=> NHH4ZYIzdU1? MorqNlRp NYnBUIFJcW6qaXLpeIV{KHSqZTDjfZRwfG:6aXPpeJkhd2Zic3nsbYJqdmmwIITvJGhmVGFiY3XscJMv M4\rb|IyQDd3M{i1
Jurkat MX\GeY5kfGmxbjDBd5NigQ>? NHHGbHEyOG2P NWLWVlBVOWh? NVu4cmI5\GWlcnXhd4V{KHSqZTDlfJBz\XO|aX;uJI9nKEyFMz3JTUBidmRidHjlJIZwem2jdHnvckBw\iCjdYTvdIhi\2:|b33ldy=> NXH2bmJIOjF6NkSwN|c>
K562 MUTGeY5kfGmxbjDBd5NigQ>? NFHUfIIyOG2P NEL5S3oycA>? M4rt[YRm[3KnYYPld{B1cGViZYjwdoV{e2mxbjDv[kBNSzNvSVmgZY5lKHSqZTDmc5Ju[XSrb36gc4Yh[XW2b4DoZYdwe2:vZYO= NUHaVmtCOjF6NkSwN|c>
Nara-H MoryS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV21cW0> NUHwS45uPDiq MUHlcohidmOnczD0[Y1{cXKxbHnteZNu\WSrYYTl[EB{fXCycnXzd4lwdiCxZjDOZZJiNUhiY3XscEBxem:uaX\ldoF1cW:w NH71TXIzOThyNUCzNy=>
HUVECs Mn7nSpVv[3Srb36gRZN{[Xl? NGW3[4YyOG2P M4L3UlAvPWh? M{jtfoRm[3KnYYPld{B1cGViQVfFMWJUSWmwZIXj[YQh[XW2b4DoZYd6KGyndnW= M4LqXFIyPDZ6NUmy
HepG2 MUjBdI9xfG:|aYOgRZN{[Xl? NH\CdlUzdU1? MVyxbC=> MWLlcohidmOnczDyZYRq[XSrb36tbY5lfWOnZDDj[YxtKGSnYYTo MmPYNlE1PTN4OUG=

... Click to View More Cell Line Experimental Data

In vivo 3-Methyladenine blocks autophagy through its effect on class III phosphatidylinositol 3-kinase (PI3K). 3-Methyladenine treatment does not alter the degree of hemorrhage compared with the subarachnoid hemorrhage (SAH) group. 3-Methyladenine pretreatment significantly aggravates neurological symptoms when compared with the SAH + vehicle group. Autophagy is decreased when 3-Methyladenine treatment is applied. Conversely, cleaved caspase-3 is markedly up-regulated in the SAH + 3-Methyladenine group. In line with the up-regulation of cleaved caspase-3 expression, the number of TUNEL-positive cells in the right cortex is significantly increased in the SAH + 3-Methyladenine group compared with the SAH + vehicle group. [5]

Protocol

Kinase Assay:[4]
+ Expand

Protein degradation assay:

HeLa cells are radiolabeled for 24 hours with 0.05 mCi/mL l-[U- 14C]valine. At the end of the labeling period, cells are rinsed three times with PBS. Cells are incubated for the designated times in either full medium or EBSS with or without the presence of 10 mM 3-Methyladenine.
Cell Research:[2]
+ Expand
  • Cell lines: HeLa cell line
  • Concentrations: 1-10 mM
  • Incubation Time: 24, 48 or 72 hours
  • Method: Cell (such as HeLa cell) viability is determined by a trypan blue exclusion assay. Briefly, after treated with 3-Methyladenine, both adherent and floating cells are collected and suspended in phosphate buffered saline (PBS, pH 7.4) at a final density of 1-2 × 106/mL. An equal volume of 0.4% trypan blue solution (w/v, in PBS) is added to the cell suspension and mixed thoroughly. After incubation at room temperature for 3 min, cell counting is performed using a hemacytometer.
    (Only for Reference)
Animal Research:[5]
+ Expand
  • Animal Models: Adult male Sprague–Dawley rats weighing 300-350 g
  • Formulation: Saline
  • Dosages: 400 nM
  • Administration: Intracerebral ventricular
    (Only for Reference)

Solubility (25°C)

In vitro Water 10 mg/mL warmed (67.04 mM)
Ethanol 4 mg/mL (26.81 mM)
DMSO 3 mg/mL warmed (20.11 mM)
In vivo Add solvents individually and in order:
water
10mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 149.15
Formula

C6H7N5

CAS No. 5142-23-4
Storage powder
Synonyms NSC 66389

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    I'm also wondering whether it can be dissolved in water,or maybe something like culture medium,normal saline solution to form 10mM solution

  • Answer:

    As the reference (http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal. pone.0035665), 3-MA, which was found to inhibit autophagy at concentrations ranging from 1 to 10 mM was directly dissolved into the culture medium at the indicated concentrations. And we tested the solubility of S2767, and found the solubility of 3-MA in DMEM is 31 mg/mL at about 40°C.

PI3K Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID