3-Methyladenine (3-MA)

Catalog No.S2767 Synonyms: NSC 66389

3-Methyladenine (3-MA) Chemical Structure

Molecular Weight(MW): 149.15

3-Methyladenine (3-MA) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation.

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4 Customer Reviews

  • MDC-labeled vacuoles were induced by AZD2014 and inhibited by autophagy inhibitor (3-MA). SMMC-7721 cells were treated with AZD2014 or rapamycin at concentrations of 100 and 600 nM, respectively, for 48 hours in the presence or absence of 3-MA, and then stained with MDC. Cells were immediately observed under a confocal microscope. Cells in the control group were treated with DMSO. bars, 20 μm.

    Am J Cancer Res, 2015, 5(1): 125-139. 3-Methyladenine (3-MA) purchased from Selleck.

    Cells were treated with DMSO, TDCIPP (100 μM) only, or pretreated with 3-MA before treatment with TDCIPP (100 μM). LC3 conversion and beclin-1 and p62 expression were assessed by western blotting at 24 h

    Food Chem Toxicol, 2017, 100:183-196. 3-Methyladenine (3-MA) purchased from Selleck.

  • PC-9/ER cells were treated with CX-4945 (5 mM) for 48 h in the presence or absence of 3-MA (2 mM) and Atg7 siRNA (100 nM). Cleavage of PARP-1 and caspase-3 was shown by Western blot analysis. CT: without CX-4945; CX: CX-4945; CX+G: CX-4945 + gefitinib; CX+E: CX-4945 + erlotinib.

    PLoS One, 2014, 9(12): e114000 . 3-Methyladenine (3-MA) purchased from Selleck.

    A: The survival ratio of AML12 cells was measured after treatment with rapamycin or 3-MA; B: Western blot was used to detect the expression of LC3 and P62 in AML12 cells treated with Rapamycin or 3-MA.

    World J Gastroenterol, 2016, 22(18): 4501-4514.. 3-Methyladenine (3-MA) purchased from Selleck.

Purity & Quality Control

Choose Selective PI3K Inhibitors

Biological Activity

Description 3-Methyladenine (3-MA) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation.
Targets
Vps34 [1]
(HeLa cells)
PI3Kγ [1]
(HeLa cells)
25 μM 60 μM
In vitro

The slight preference for Vps34 prevention by 3-Methyladenine probably arises from a hydrophobic ring specific to Vps34, which encircles the 3-methyl group of 3-Methyladenine. [1] 3-Methyladenine has been reported to cause cancer cell death under both normal and starvation conditions. 3-Methyladenine could also suppress cell migration and invasion independently of its ability to inhibit autophagy, implying that 3-Methyladenine possesses functions other than autophagy suppression. 3-Methyladenine elicits caspase-dependent cell death that is independent of autophagy inhibition. Treatment with 5 mM 3-Methyladenine reduces the percentage of glucose-starved HeLa cells displaying GFP-LC3 puncta to 23%. The levels of LC3-I are increasing and the levels of LC3-II are decreasing between 12 and 48 hours in cells that are treated with 3-Methyladenine. Conversion of LC3-I to LC3-II is suppressed by 3-Methyladenine. Treatment of HeLa cells with 3-Methyladenine at 2.5 mM or 5 mM for one day does not affect cell viability, whereas treatment with 10 mM 3-Methyladenine for one day causes a 25.0% decrease in cell viability. Treatment of cells with 2.5, 5 or 10 mM 3-Methyladenine for two days causes 11.5%, 38.0% and 79.4% decrease in viability, respectively. 3-Methyladenine decreases cell viability in a time- and dose-dependent manner. 3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest. [2] Suppression of autophagy by 3-Methyladenine inhibits SU11274-induced cell death. [3] Prolonged treatment with 3-Methyladenine (up to 9 hours) induces significant LC3 I to II conversion in wild type MEFs. Prolonged treatment with 3-Methyladenine, but not wortmannin, markedly increases GFP-LC3 punctuation/aggregation. 3-Methyladenine-induced LC3 conversion and free GFP liberation are ATG7-dependent. 3-Methyladenine treatment leads to evident increase of p62 protein level. 3-Methyladenine increases the p62 level even in Atg5−/− MEFs as well as in cells with DOX-mediated deletion of ATG5. 3-Methyladenine inhibits class I and class III PI3K in different temporal patterns. 3-Methyladenine-induced LC3 I to LC3 II conversion is dramatically compromised in Tsc2−/− cells compared with wild type cells.3-Methyladenine disrupts the anti-autophagic function of mTOR complex 1. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
95D NYXZZnp5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUToO2FMO22P MnvFNog> MX\EUXNQ NXjkeHhlemWmdXPld{Boem:5dHigbY5pcWKrdH;yfUBm\m[nY4Sgc4YhSkSPQx?= NFnoUXIzPTdzNkW2NS=>
A549  NFr3[ZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXLtUoFFO22P MkiwNog> M4f4OWROW09? MVLy[YR2[2W|IHfyc5d1cCCrbnjpZol1d3K7IHXm[oVkfCCxZjDCSG1E NGPwSY8zPTdzNkW2NS=>
95D MlPURZBweHSxc3nzJGF{e2G7 M{jwclVuVQ>? M3HjNFJp MoTZSG1UVw>? NWLvd4l4cW6qaXLpeJMhSkSPQz3pcoR2[2WmIHHwc5B1d3SrYzDj[YxtKGSnYYTo NInNZZgzPTdzNkW2NS=>
A549  MmDaRZBweHSxc3nzJGF{e2G7 M{PSO|VuVQ>? MYOybC=> NX7JdVF3TE2VTx?= M1i3Z4lvcGmkaYTzJGJFVUNvaX7keYNm\CCjcH;weI91cWNiY3XscEBl\WG2aB?= Ml7vNlU4OTZ3NkG=
NBL-W-S  NETmVlZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVfSfZBqOW2P NHv4PYY3cA>? NF35PYFmdmijbnPld{BISU6WLU[xJJRwgGmlaYT5 M2nYVFI2OzJ|MkKy
NBL-W-S  M1HUZ2Fxd3C2b4Ppd{BCe3OjeR?= MWKxcW0> NUDGclR7Pmh? M2CxPYlv[3KnYYPld{Bk\WyuIHHwc5B1d3OrczDpcoR2[2WmIHL5JGdCVlRvNkG= NXHoOWNrOjV|MkOyNlI>
A2780cp  MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVm0WHV2OW2P Ml;HNYg> NHnQd5ZqdmO{ZXHz[ZMh[2m|cHzheIlvNWmwZIXj[YQh[2WubDDk[YF1cA>? NVHGZpBGOjV|MkK2PVQ>
A549  MknnSpVv[3Srb36gRZN{[Xl? M2DVdlVuVQ>? MnPuNog> NYqzPFFDe3WycILld5NmeyCVUFOgbY5lfWOnZDDhZ4N2dXWuYYTpc44hd2ZiTFOzMWlK M{WxU|I2Ojh3NkK4
H157 NVW5VnVFTnWwY4Tpc44hSXO|YYm= M3f4fFVuVQ>? NEHSd2gzcA>? M{npTJN2eHC{ZYPz[ZMhW1CFIHnu[JVk\WRiYXPjeY12dGG2aX;uJI9nKEyFMz3JTS=> M1G4XVI2Ojh3NkK4
MDR MkXERZBweHSxc3nzJGF{e2G7 MWKxNI1O M13pdFZp M3jrRZN1emWwZ4To[Y5{KHSqZTDwc5dmeiCxZjDhcpRq[2GwY3XyJIRzfWe| NFjwbHkzPTBzOUewNS=>
HT-29  NV\sc4xpSXCxcITvd4l{KEG|c3H5 NWex[FJwOW2P MoLtOFhp MlTFSG1UVw>? Mo\i[Y5p[W6lZYOgZo9zfGW8b33pZk1qdmS3Y3XkJINmdGxidnnhZoltcXS7IHzvd5M> NILLPFQzPDh2MkG1PC=>
Microglia  NXvs[pV1SXCxcITvd4l{KEG|c3H5 M1TYdFVuVQ>? NWHJZYliOjSq MUHk[YNz\WG|ZYOgbJlxd3irYT3pcoR2[2WmIHPlcIwh\GWjdHi= M4TFVlI1QDF6NkCx
A2780cp NHXpbZlCeG:ydH;zbZMhSXO|YYm= NH[4fJAzNjWvTR?= MYexbC=> NUnjcYZE\GSKMl:= M4\DZoVvcGGwY3XzJINqe3CuYYTpck1qdmS3Y3XkJINmdGxiZHXheIg> MmfxNlQ5OTd7NE[=
HepG2 MmP4SpVv[3Srb36gRZN{[Xl? NXX5NYxUPW2P MmTqOIg> M4TvdYlv[3KnYYPld{BJVCC{ZXzlZZNm NILubZUzPDdzM{W4Oy=>
A549 Ml7ERZBweHSxc3nzJGF{e2G7 M4mxW|VuVQ>? NFn3cIw1QGh? M3\Ie4Rm[3KnYYPld{B1cGVicHXyZ4VvfGGpZTDv[kBk\WyuIHTlZZRpKGGwZDDlfJBz\XO|aX;uJIxmfmWuczDv[kBk[XOyYYPlMVMtKEKnY3zpck0yKGGwZDDMR|MuUUl? MVKyOFcxPjNyMx?=
U2OS M2rUfGFxd3C2b4Ppd{BCe3OjeR?= NWi3dIc5OTCvTR?= NInrRXIzPGh? MnOzbY51\W6|aX\p[ZMhfGinIHfyc5d1cCCrbnjpZol1cW:wIH;mJJRp\SCXMl;TJINmdGy|IHnu[JVk\WRiYomgSI95 NYr0RmN3OjR4M{mwNVM>
Saos-2 M2npO2Fxd3C2b4Ppd{BCe3OjeR?= M3LmWVExdU1? NUHBOmlWOjSq NHzQe2lqdnSnboPp[olmeyC2aHWg[5Jwf3SqIHnubIljcXSrb36gc4YhfGinIGWyU3Mh[2WubIOgbY5lfWOnZDDifUBFd3h? MnruNlQ3OzlyMUO=
A549 MnnaRZBweHSxc3nzJGF{e2G7 NWTJZ3JpOTCvTR?= MonQOFhp NYHB[W1H[WOlZXzldoF1\XNidHjlJJJm\HWldHnvckBw\iClZXzsJJZq[WKrbHn0fUBqdmS3Y3XkJIJ6KFCWWB?= NUfNZ2FvOjR4Mk[3NlI>
HCT116  MmfqRZBweHSxc3nzJGF{e2G7 NULiPVJYPW2P MnfLNlRp MXPlcohidmOnczD0bIUh[XCxcITvd4l{KGmwZIXj[YQh[nliYYDp[4VvcW5? NUjWXW1lOjR4Mk[1NlI>
HGC-27 MW\GeY5kfGmxbjDBd5NigQ>? MoPxNVBuVQ>? Mn\LNYg> NEHQSHdqdmirYnn0d{B1cGViY3XscEB3cWGkaXzpeJkhdG:|czDifUBTSURyMEGgc5IhVUtvMkKwOi=> MUGyOFQyPjN2OR?=
U2OS  MWnGeY5kfGmxbjDBd5NigQ>? NU\UWFZROC53L{HtUS=> NWLmfmQ5PDiq MX3pcoR2[2W|IIPhcIlvd227Y3nuMYlv\HWlZXSgZ4VtdCC4aXHibYxqfHlibH;zdy=> NVXXVnY1OjR|NUizOFI>
MG-63 MWTGeY5kfGmxbjDBd5NigQ>? MY[wMlUwOW2P NVrMXYJXPDiq M4\xSIlv\HWlZYOgd4FtcW6xbYnjbY4ucW6mdXPl[EBk\WyuII\pZYJqdGm2eTDsc5N{ NXXGdHdUOjR|NUizOFI>
MG-63 NG[2NXVCeG:ydH;zbZMhSXO|YYm= NGH0cJYxNjVxMX3N M1HxXFMzcA>? M4PUS4VvcGGwY3XzJJNidGmwb335Z4lvNWmwZIXj[YQh[2WubDDhdI9xfG:|aYO= MWqyOFM2QDN2Mh?=
MG-63 NW\hSXJQSXCxcITvd4l{KEG|c3H5 MV:xNI1O NYPkV3d3OTKq Mnm0[Y5p[W6lZYOgSHAucW6mdXPl[EBieG:ydH;zbZM> MVyyOFM2QDNyMR?=
NTUB1 NXrZdXdESXCxcITvd4l{KEG|c3H5 NHP1UXA2dU1? NH30N2EyNjWq NULzc4JReG:2ZX70bYF1\XNiY3Xs[YNwgGmkLXnu[JVk\WRiYYDvdJRwe2m| NEDZRpAzPDN2OUG3Oi=>
T24 M4HneGFxd3C2b4Ppd{BCe3OjeR?= NFHHWG82dU1? M2PRZlEvPWh? Mo\EdI91\W62aXH0[ZMh[2WuZXPvfIljNWmwZIXj[YQh[XCxcITvd4l{ NYLsUpR4OjR|NEmxO|Y>
SGC-7901  NFTIfnJCeG:ydH;zbZMhSXO|YYm= MYKycW0> NUTJRmY1OWh? NHrscoFqdmO{ZXHz[ZMhS0FvNDDpcoR2[2WmIHHwc5B1d3Orcx?= NHzKNZczPDN{MUO0NC=>
SMMC-7721 M3HpZmFxd3C2b4Ppd{BCe3OjeR?= MYmycW0> NF;xUnkycA>? M3j4OYlv[3KnYYPld{BESS12IHnu[JVk\WRiYYDvdJRwe2m| MXuyOFMzOTN2MB?=
ECSCs  MlPPRZBweHSxc3nzJGF{e2G7 NE\3O5MyOG2P MlW4OIg> NVnCZWli\GWlcnXhd4V{KHKjcHHtfYNqdi22cnXheIVlKGGyb4D0c5Nqew>? NVnZUGV4OjR|MUmxNFk>
MCF-7  NHXwNHNHfW6ldHnvckBCe3OjeR?= M3;Pd|ExdU1? MUSyOIg> NWrXOJhwcW6qaXLpeJMhfGinIHH1eI9xcGGpeTDpcoR2[2WmIHL5JINp\W2xdHjldoFxgSCmcoXndy=> NVK2em1FOjR|MUW1O|g>
UOK257 MX3BdI9xfG:|aYOgRZN{[Xl? MoixOY1O M1\RSlNp M4HhZoVvcGGwY3XzJJBi[2yrdHH4[YwudWWmaXH0[YQh[XCxcITvd4l{yqB? M3zhNlI1OzB3NkC0
ACHN-5968 MoPFRZBweHSxc3nzJGF{e2G7 M3zKcFVuVQ>? NGLwepU{cA>? NEHiRppmdmijbnPld{Bx[WOuaYThfIVtNW2nZHnheIVlKGGyb4D0c5Nqe8Li NYXZfpFvOjR|MEW2NFQ>
Huh7 NGfVNXhCeG:ydH;zbZMhSXO|YYm= NEf5flczdU1? MoXRNVJp M2nRNGROW09? MmLSbY5pcWKrdIOgRXpFQDB3NT3pcoR2[2WmIHPlcIwh\GWjdHi= MonQNlQzQTd|MEC=
Hep3B M2DVcmFxd3C2b4Ppd{BCe3OjeR?= NWH3eINtOm2P NFe3eYUyOmh? NGPydJRFVVOR M1vSSYlvcGmkaYTzJGFbTDhyNUWtbY5lfWOnZDDj[YxtKGSnYYTo MWWyOFI6PzNyMB?=
RKO MlHmSpVv[3Srb36gRZN{[Xl? M1O4WlJuVQ>? M2\SXlFp NFrrc2lFVVOR NHfodmhmdmijbnPld{Bk\WyuIHTlZZRpKGK7IHflcIRidmGveXPpci=> NYLNPJVZOjR{OUG3O|c>
HepG2 E47 MkfwSpVv[3Srb36gRZN{[Xl? NFz1RW8zNjWvTR?= NVTKN2J[PDiq NYXtUXBScW6lcnXhd4V{KHSqZTD0c5hq[2m2eTDv[kBCSSxiQmPPMEBidmRiQ1PsOC=> NF7TUpczPDJ5M{ezPC=>
LoVo  MU\BdI9xfG:|aYOgRZN{[Xl? NED3PIU2dU1? NUHN[FllPDiq NF\LVY5mdmijbnPld{BFS0FvaX7keYNm\CCjcH;weI9{cXNw NV\EfGxQOjR{MEG4NVI>
WiDr NGjjRYRHfW6ldHnvckBCe3OjeR?= NWDOZW5SOTCvTR?= M2TCSlFp MonGbY5pcWKrdIOgVGNDVC2rbnT1Z4VlKEyFMzDJTUBmgHC{ZYPzbY9v MmjLNlQyQTB2OEm=
H1299  NYP0fnNWTnWwY4Tpc44hSXO|YYm= NXHRS2hTOTCvTR?= M3j4WlRp NYry[ncxcW6lcnXhd4V{KGOrc4DsZZRqdi2rbnT1Z4VlKGOnbHyg[IVifGh? NHTqNYwzPDF5M{KwPC=>
H460 MXfGeY5kfGmxbjDBd5NigQ>? NVrifGxWOTCvTR?= NWXGO41iPGh? NYC0eGNMcW6lcnXhd4V{KGOrc4DsZZRqdi2rbnT1Z4VlKGOnbHyg[IVifGh? Mo\TNlQyPzN{MEi=
A549 NWe4V25TTnWwY4Tpc44hSXO|YYm= M3mwcFExdU1? M1;KPVRp NWDT[Y9{cW6qaXLpeJMh[XW2b4DoZYd6KGmwZIXj[YQh[nliaYLyZYRq[XSrb36= MWGyOFE1Ojd|NR?=
Hep3B NIC4WJRCeG:ydH;zbZMhSXO|YYm= M3\ObFVuVQ>? NX;5c|RbOjSq NU\QU4Rb[XS2ZX71ZZRmeyCWTl[t{tEheHKxdHXjeIlwdiCjZ3HpcpN1KHOncoXtJJN1[XK4YYTpc44udWWmaXH0[YQh[XCxcITvd4l{ NEmxPIQzPDB4Nk[5Ny=>
SMMC-7721 NGToWHJCeG:ydH;zbZMhSXO|YYm= Mo\3OY1O M{n2V|I1cA>? MmnLZZR1\W63YYTld{BVVkZvzsGgdJJwfGWldHnvckBi\2GrboP0JJNmenWvIIP0ZZJ3[XSrb36tcYVlcWG2ZXSgZZBweHSxc3nz NHrVUGozPDB4Nk[5Ny=>
HO8910 Mn7RRZBweHSxc3nzJGF{e2G7 NHj0fowyOG2P NUn4UVV2OTKq NVTuRY97\W6qYX7j[ZMhSjF7LXnu[JVk\WRiYYDvdJRwe2l? NIThUlUzOzl6M{[xNC=>
MCF7 M1LqSmZ2dmO2aX;uJGF{e2G7 NEDUdok2dU1? M3\MTVI1cA>? M2PkXolv[3KnYYPld{BEfU9iaX7keYNm\CClZXzsJIRm[XSq NW\xR3lLOjN7NkK2Nlk>
HONE-1  M4P2dGZ2dmO2aX;uJGF{e2G7 M3u3clVuVQ>? NXjaflRyOWh? NEL3WpRz\XC{ZYPz[ZMhPnJvbXXkbYF1\WRiUl;TJJBzd2S3Y4Tpc44> M2H5bFI{QDl{M{W4
HeLa NWL4UIZHTnWwY4Tpc44hSXO|YYm= MnHVNVBuVQ>? MXWybC=> NXHNUoN2e3WycILld5NmeyCOQ{OgTWkh\XiycnXzd4l{d25? M{nud|I{QDZ2N{O4
HepG2 MWXGeY5kfGmxbjDBd5NigQ>? NGO4OYsyOG2P NV72TVBJOjSq MVPpcohq[mm2czDzbXRKT0GULTDhcoQhUEKVUz3pcoR2[2WmIHH1eI9xcGGpeR?= NYTxR4JLOjN6MUewOFA>
SH-SY5Y MoqySpVv[3Srb36gRZN{[Xl? MoHMNY1O M{XVRVI1cA>? M2jqd4lvcGmkaYTzJJRp\SCjdYTvdIhi\3liaX7keYNm\CCkeTDUU2NR NU[yS|Y{OjN5NEOxOFg>
SH-SY5Y NVHsZnJ7SXCxcITvd4l{KEG|c3H5 NEDXfmY2dU1? MYSxbC=> MXvhZo9tcXOqZYOgZ4Vt[XO2cn;sJI5mfXKxcILveIVkfGm4ZTDl[oZm[3R? MY[yN|YyQTN7NR?=
PC12  NH\mN5hHfW6ldHnvckBCe3OjeR?= M{HQW|ExdU1? MmDlNlRp MUX3ZZRmeg>? NVnneFJZyqCrbnjpZol1eyClaIntc5RzgXC|aX6tcIls\SCycn;0[YF{d22jbDDhZ5Rqfmm2eT6= M13qO|I{PjB|OUe5
OV2008 M3H1dGFxd3C2b4Ppd{BCe3OjeR?= NWXt[lFMPW2P NGK3UWczPGh? Ml3CZ49vfmW{dIOgSnR[PzJyIIfpeIghS0SGUDDpcpRwKGGwIHHk[Il1cX[nIHXm[oVkfCC2b4fhdoR{KGurbHzpcochd3[jcnnhckBk[W6lZYKgZ4VtdHN? MlfhNlM2QTJ{OEG=
A2780 M{\UVmFxd3C2b4Ppd{BCe3OjeR?= NF7vPG82dU1? MViyOIg> NGjzNG9kd264ZYL0d{BHXFl5MkCge4l1cCCFRFTQJIlvfG9iYX6gZYRlcXSrdnWg[YZn\WO2IITve4Fz\HNia3nscIlv\yCxdnHybYFvKGOjbnPldkBk\Wyucx?= NGXpNmQzOzV7MkK4NS=>
Saos-2  MUXBdI9xfG:|aYOgRZN{[Xl? NV\ielZyOW2P MUm5Oog> MlHWbY5kemWjc3XzJINmdGxiZHXheIghcW6mdXPl[EBjgSCSQ2i= M1TyNlI{PTZ|MUex
1321N1 NGewNZpEgXSxdH;4bYNqfHliQYPzZZk> MnrVOY1O MYeyOIg> M4rITZBzd3SnY4TzJINmdGxiYXfhbY5{fCCSQ16tbY5lfWOnZDD0c5hq[2m2eR?= NYXETFB6OjN3MkWyOlU>
SH-SY5Y M1[3VmN6fG:2b4jpZ4l1gSCDc4PhfS=> M{PTW|VuVQ>? MUWyOIg> NGGzXlBqdmO{ZXHz[ZMhWEOQIITvfIlkcXS7 NYfIblV1OjN3MkWyOlU>
HT-29  M3TnbmZ2dmO2aX;uJGF{e2G7 NUHHOol5OW2P M4D6dFQ5Nzl4aB?= MVnpcohq[mm2czDBUXBMKGmwZIXj[ZMh[XW2b4DoZYdq[yClZXzsJIRm[XSq M1POUlI{PTB6Mkey
OR6 MonXSpVv[3Srb36gRZN{[Xl? MYixNI1O NIruZ2I4Omh? MUPzeZBxemW|c3XzJGhEXiC{ZYDsbYNifGmxbjDhcoQh\m:{bXH0bY9vKG:oIHH1eI9xcGGpb4PvcYV{ MV[yN|M6PTh5NR?=
Hela  Moe3SpVv[3Srb36gRZN{[Xl? Ml7nOY1O MlXkNlRp MV\pcohq[mm2czDzeIFzfmG2aX;uMYlv\HWlZXSgZZV1d3CqYXf5 NWfIXZZ4OjN|OUW2O|k>
MCF-7  MYLGeY5kfGmxbjDBd5NigQ>? MVG1cW0> MmSyNlRp NXLEO2VkcW6qaXLpeJMhe3Sjco\heIlwdi2rbnT1Z4VlKGG3dH;wbIFogQ>? NFnuTYczOzN7NU[3PS=>
HUVECs NWPKfnhCTnWwY4Tpc44hSXO|YYm= Mmn5N41O NX3SWFR{OjSq NX7nTYw1[myxY3vzJJRp\SCycn;0[YN1cX[nIHXm[oVkfCCxZjDy[ZN3\XKjdILvcEBjgSCrbnjpZol1cW6pIHH1eI9xcGGpeR?= MXeyN|M2QDl{OB?=
T24 NHyzZ5BHfW6ldHnvckBCe3OjeR?= MVWxNI1O MYqxbC=> NVTr[FROemWmdXPld{B1cGViY3zlZZZi\2Vib3[gUGM{KGGodHXyJIJicWOjbHnuJJRz\WG2bXXueC=> M1\YOFI{OzV2MEiw
U251MG  MYnGeY5kfGmxbjDBd5NigQ>? MUOzcW0> NF7vdHQycA>? M{LJc5N2eHC{ZYPz[ZMhVEN|LVnJJJBzd3SnaX6g[ZhxemW|c3nvci=> MkTTNlM{Ozh4MUi=
GTL-16  MXnBdI9xfG:|aYOgRZN{[Xl? NWLpWVdmPW2P MWeyOIg> Mo\CdoVlfWOnczDj[YxtKH[rYXLpcIl1gSCjczDjc41x[XKnZDD0c{Bk\WyuczD0doVifGWmIIfpeIghVUWWIHnubIljcXSxcoO= MUmyN|MyOzR7MB?=
T-47D NX\2XGUzTnWwY4Tpc44hSXO|YYm= NIGxS4gyOG2P NYPHUoI2Omh? NWP5dml7cW6qaXLpeJMh[XW2b4DoZYd6KHC{b3Pld5Mh[W6mIHnuZ5Jm[XOnczDyZZBidXmlaX6gbY5lfWOnZDDhdI9xfG:|aYO= MYGyN|MxODB{Nh?=
PaCa44 NELCNVRCeG:ydH;zbZMhSXO|YYm= MUWyMlVuVQ>? NH7MfZIycA>? MofsdoVlfWOnc9Mg[4VvcXCrbj3t[YRq[XSnZDDhdI9xfG:|aYO= NUnrOFZqOjNzMkSxNVI>
MDA-MB231 MmLrSpVv[3Srb36gRZN{[Xl? M2jITFVuVQ>? MnLBNYg> NIrhNXZqdmO{ZXHz[ZMhemW|dnXyZZRzd2xvbXXkbYF1\WRiY3HzdIF{\SCjY4TpeoF1cW:wIHHu[EBk\WyuIHTlZZRp MY[yN|A5QDh3MB?=
HeLa M2\scmFxd3C2b4Ppd{BCe3OjeR?= Mon1NVBuVQ>? NWn2R2t3Omh? NF7BR4Zl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgZ48ufHKnYYTt[Y51KHerdHigVGVK M3fqOlI{ODByMUO1
SK-HEP-1 M4i3XGFxd3C2b4Ppd{BCe3OjeR?= NXXucFNEOTCvTR?= MkfSNYg> NVK4WmV3eHKxdHXjeJMh[WejaX7zeEBifXSxcHjh[5kh[W6mIHnu[JVk\XNiYYDvdJRwe2m|IHnuJIJ2\mGuaX6teJJm[XSnZDDj[Yxtew>? NInJdHozOjh3OE[0PS=>
HepG2 M{fvT2Fxd3C2b4Ppd{BCe3OjeR?= NWrheoZmO22P MmXmOYg> MlfPdoVlfWOnczDj[YxtKGGyb4D0c5NqeyCrbnT1Z4VlKGK7IGHEdy=> NGjQOGozOjh|NkW5OS=>
MCF-7  NHzHWGJHfW6ldHnvckBCe3OjeR?= MVeycW0> MWeyOIg> MV;pcohq[mm2czDheZRweGijZ4mgbY5lfWOnZDDifUBVVcLi NV3XUI5lOjR7N{C2O|Y>
MDA-MB-231 NI\vcGtHfW6ldHnvckBCe3OjeR?= Ml3BNo1O MUOyOIg> MVXpcohq[mm2czDheZRweGijZ4mgbY5lfWOnZDDifUBVVcLi NWDy[I1IOjR7N{C2O|Y>
MCF-7  MUfGeY5kfGmxbjDBd5NigQ>? MXWycW0> NVnLbG5nPDiq M3;aepBzd22xdHXzJHRONWmwZIXj[YQh[2WubDDk[YF1cA>? M4j5eVI1QTdyNke2
MDA-MB-231 NIj3cmRHfW6ldHnvckBCe3OjeR?= M13rbFJuVQ>? NXHIRXFlPDiq M13XSZBzd22xdHXzJHRONWmwZIXj[YQh[2WubDDk[YF1cA>? MkDtNlQ6PzB4N{[=
PANC-1  M3nle2Fxd3C2b4Ppd{BCe3OjeR?= Mly4NY1O NVy3NoFWPDiq NV3CTmpTTE2VTx?= M4i3[4VvcGGwY3XzJIJwenSnen;tbYIucW6mdXPl[EBk\WyuII\pZYJqdGm2eTDsc5N{ NVvIfplFOjR6NEKxOVg>
MDA-MB 231 NImzeYNCeG:ydH;zbZMhSXO|YYm= Mn7jOY1O M2PJcVAvPWh? M{OwTI1w\HWuYYTld{BVd2OxbXnuxs7DqGmwZIXj[YQh[XCxcITvd4l{ MV[yOFg{ODd6MR?=
Microglia  M3TLb2Fxd3C2b4Ppd{BCe3OjeR?= NEPBeGc2dU1? NV7JWol6OjSq MWrk[YNz\WG|ZYOgbJlxd3irYT3pcoR2[2WmIHPlcIwh\GWjdHi= MYmyOFgyQDZyMR?=
A2780cp M3fwRWFxd3C2b4Ppd{BCe3OjeR?= M{[3SFIvPW2P NHe4TVMycA>? M2fCXYRlUDKR MX3lcohidmOnczDjbZNxdGG2aX6tbY5lfWOnZDDj[YxtKGSnYYTo NIPXRlczPDhzN{m0Oi=>
HepG2 MmmzSpVv[3Srb36gRZN{[Xl? NX;BT3V5PW2P NX7ucnNEPGh? M4TIW4lv[3KnYYPld{Bk\WyudXzhdkBt\X[nbIOgc4YhUE{EoB?= M4qyNFI1PzF|NUi3
U2OS MlTpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmXxNVBuVQ>? NYTEdJZTOjSq M4O1O4lvfGWwc3nmbYV{KHSqZTDndo94fGhiaX7obYJqfGmxbjDpcoR2[2WmIHL5JGRwgA>? NXPq[oRGOjR4M{mwNVM>
HCT116 M3u2[WFxd3C2b4Ppd{BCe3OjeR?= NUO5XHZjPW2P MmjPNlRp NHPoZZpFVVOR NUXoZmI4\W6qYX7j[ZMh[XCrZ3XubY4ucW6mdXPl[EBk\WyuIHTlZZRp MVqyOFYzPjV{Mh?=
MCF-7  MnW5RZBweHSxc3nzJGF{e2G7 M2W4Z|AvOW2P MYK2bC=> Mkjt[Y5p[W6lZYOgd4lzfGmwb3ytbY5lfWOnZDDhdI9xfG:|aYO= NH7DNFIzOjd3MUm4PS=>
PC-3  MkLhRZBweHSxc3nzJGF{e2G7 M3;OcVJuVQ>? NUjuN4NOOmh? MmHmbY5kemWjc3XzJG9TUS2rbnT1Z4VlKGOnbHyg[IVifGh? NVrvPJZ7OjJ5NEW1PFA>
U251  MVHBdI9xfG:|aYOgRZN{[Xl? MYW1cW0> NVH4OnN{OjSq M2nLPIlv[3KnYYPld{BUOS2rbnT1Z4VlKGOnbHyg[IVifGh? Ml\tNlI2Pzl5OEi=
HeLa  MXXBdI9xfG:|aYOgRZN{[Xl? NXzMW2FiPW2P M2njcVI1cA>? NFywcHdqdmS3Y3XzJINie3Cjc3Wt[IVx\W6mZX70JINmdGxiZHXheIg> M1j3VlIzPTR3MUK4
A549 NWjoXmNbTnWwY4Tpc44hSXO|YYm= NGO0bmoxNjGvTR?= NHKxZlAzPGh? Ml7Kd5VxeHKnc4Pld{BUXTFzMke0MYlv\HWlZXSgZ4VtdCCmZXH0bC=> Moi2NlI1PjZ7NkC=
pDCs MWDGeY5kfGmxbjDBd5NigQ>? MYqxNI1O Mn3YNE42cA>? MWdCpJJm\HWlZYOgeIhmKGmwZIXjeIlwdiCxZjDJSm4u|rFiYomgd5NTVkF2MB?= MnrkNlI{QTZ3OUm=
BGC-823 NYDVU2RrTnWwY4Tpc44hSXO|YYm= MoDGOY1O NGnkT40zcA>? NE[2TGFqdmirYnn0d{B1cGVicnH0[UBw\iCjdYTvdIhi\2mlIHPlcIx{ M2jaT|IzOzJ{MUWy
U937 NWmzSJZ{TnWwY4Tpc44hSXO|YYm= NF\RT5gzdU1? NYT0SIlZOTKq MoL0[IVkemWjc3XzJJRp\SCjdYTvdIhi\3licnH0bY/DqA>? M4DUNVIzOTV3MUWw
Marc-145 NI\OWoFHfW6ldHnvckBCe3OjeR?= NX3yb4tRPW2P NFXBWJUyOi9{ND:zOog> M3P1ZpJm\HWlZYOgeIhmKFCUUmPWJJRqfGW{czDhcoQhfGinIIDyc5RmcW5iZYjwdoV{e2mxbh?= Mnv0NlIyOTl7MEC=
HBx  NVv3XolCSXCxcITvd4l{KEG|c3H5 NFrTfG0yOG2P NITMdZo1QGh? NHW1N3dFVVOR M1TIcolv[3KnYYPld{Bk\WyuIHTlZZRp M1frV|IzODJyMEe4
MCF-7 MWTGeY5kfGmxbjDBd5NigQ>? NXz1e|hNOTCvTR?= M363OlQ5cA>? NGnsNHJjdG:la4OgZZV1d3CqYXf5JIlv\HWlZXSgZpkh[m:{dHX6c41q[g>? M3\ibFIyQTNzOUO3
RMPI8226  MkjRSpVv[3Srb36gRZN{[Xl? NUnRVXFNPW2P NVLrc5RsOWh? M{X3fZN2eHC{ZYPz[ZMhfGinIHzleoVtKG:oIHH1eI9xcGGpeTD1coRmeiCwdYTybYVvfCCmZYDs[ZRqd25? MWCyNVkyPTZ{MB?=
PC12/TetOn MUfGeY5kfGmxbjDBd5NigQ>? M4PTTFAvOS9zbV2= NI\GSXAyQGh? NX\uOGhKdGWjZIOgeI8h|rFvc4nuLHdVMSCjY3P1cZVt[XSrb36sJJRwgGmlaYT5MEBidmRib3zp[49u\XJiZn;ycYF1cW:wwrC= NIXBeXAzOTlyNk[1PS=>
HeLa  NV3WV4V2S3m2b4TvfIlkcXS7IFHzd4F6 NY\KR|A4Om2P NYnDR2NPOjSq Ml;XbY5pcWKrdHXzJJRp\SCleYTveI95cWOrdImgc4Yhe2muaXLpcolvKHSxIFjlUIEh[2WubIOu MWeyNVg4PTN6NR?=
Jurkat NWqyWJBCTnWwY4Tpc44hSXO|YYm= NIrEeGoyOG2P NFHHeHcycA>? NXToW3Y5\GWlcnXhd4V{KHSqZTDlfJBz\XO|aX;uJI9nKEyFMz3JTUBidmRidHjlJIZwem2jdHnvckBw\iCjdYTvdIhi\2:|b33ldy=> NGfyZXYzOTh4NECzOy=>
K562 NEHtenpHfW6ldHnvckBCe3OjeR?= MVGxNI1O Mo[xNYg> M{\hNoRm[3KnYYPld{B1cGViZYjwdoV{e2mxbjDv[kBNSzNvSVmgZY5lKHSqZTDmc5Ju[XSrb36gc4Yh[XW2b4DoZYdwe2:vZYO= M{fHR|IyQDZ2MEO3
Nara-H NUnvbG04T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHXKfVA2dU1? NUDEboVSPDiq MVnlcohidmOnczD0[Y1{cXKxbHnteZNu\WSrYYTl[EB{fXCycnXzd4lwdiCxZjDOZZJiNUhiY3XscEBxem:uaX\ldoF1cW:w NUTxd5JWOjF6MEWwN|M>
HUVECs MVzGeY5kfGmxbjDBd5NigQ>? MmD6NVBuVQ>? MlroNE42cA>? MoDY[IVkemWjc3XzJJRp\SCDR1WtRnNCcW6mdXPl[EBifXSxcHjh[5khdGW4ZR?= NYDnOIppOjF2Nki1PVI>
HepG2 MkPERZBweHSxc3nzJGF{e2G7 NInUbmYzdU1? M3nFUFFp M4X0XIVvcGGwY3XzJJJi\GmjdHnvck1qdmS3Y3XkJINmdGxiZHXheIg> MmToNlE1PTN4OUG=

... Click to View More Cell Line Experimental Data

In vivo 3-Methyladenine blocks autophagy through its effect on class III phosphatidylinositol 3-kinase (PI3K). 3-Methyladenine treatment does not alter the degree of hemorrhage compared with the subarachnoid hemorrhage (SAH) group. 3-Methyladenine pretreatment significantly aggravates neurological symptoms when compared with the SAH + vehicle group. Autophagy is decreased when 3-Methyladenine treatment is applied. Conversely, cleaved caspase-3 is markedly up-regulated in the SAH + 3-Methyladenine group. In line with the up-regulation of cleaved caspase-3 expression, the number of TUNEL-positive cells in the right cortex is significantly increased in the SAH + 3-Methyladenine group compared with the SAH + vehicle group. [5]

Protocol

Kinase Assay:[4]
+ Expand

Protein degradation assay:

HeLa cells are radiolabeled for 24 hours with 0.05 mCi/mL l-[U- 14C]valine. At the end of the labeling period, cells are rinsed three times with PBS. Cells are incubated for the designated times in either full medium or EBSS with or without the presence of 10 mM 3-Methyladenine.
Cell Research:[2]
+ Expand
  • Cell lines: HeLa cell line
  • Concentrations: 1-10 mM
  • Incubation Time: 24, 48 or 72 hours
  • Method: Cell (such as HeLa cell) viability is determined by a trypan blue exclusion assay. Briefly, after treated with 3-Methyladenine, both adherent and floating cells are collected and suspended in phosphate buffered saline (PBS, pH 7.4) at a final density of 1-2 × 106/mL. An equal volume of 0.4% trypan blue solution (w/v, in PBS) is added to the cell suspension and mixed thoroughly. After incubation at room temperature for 3 min, cell counting is performed using a hemacytometer.
    (Only for Reference)
Animal Research:[5]
+ Expand
  • Animal Models: Adult male Sprague–Dawley rats weighing 300-350 g
  • Formulation: Saline
  • Dosages: 400 nM
  • Administration: Intracerebral ventricular
    (Only for Reference)

Solubility (25°C)

In vitro Water 10 mg/mL warmed (67.04 mM)
Ethanol 4 mg/mL (26.81 mM)
DMSO 3 mg/mL warmed (20.11 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 149.15
Formula

C6H7N5

CAS No. 5142-23-4
Storage powder
in solvent
Synonyms NSC 66389

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

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Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    I'm also wondering whether it can be dissolved in water,or maybe something like culture medium,normal saline solution to form 10mM solution

  • Answer:

    As the reference (http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal. pone.0035665), 3-MA, which was found to inhibit autophagy at concentrations ranging from 1 to 10 mM was directly dissolved into the culture medium at the indicated concentrations. And we tested the solubility of S2767, and found the solubility of 3-MA in DMEM is 31 mg/mL at about 40°C.

PI3K Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID