3-Methyladenine (3-MA)

Catalog No.S2767 Synonyms: NSC 66389

3-Methyladenine (3-MA) Chemical Structure

Molecular Weight(MW): 149.15

3-Methyladenine (3-MA) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation.

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In DMSO USD 64 In stock
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4 Customer Reviews

  • MDC-labeled vacuoles were induced by AZD2014 and inhibited by autophagy inhibitor (3-MA). SMMC-7721 cells were treated with AZD2014 or rapamycin at concentrations of 100 and 600 nM, respectively, for 48 hours in the presence or absence of 3-MA, and then stained with MDC. Cells were immediately observed under a confocal microscope. Cells in the control group were treated with DMSO. bars, 20 μm.

    Am J Cancer Res, 2015, 5(1): 125-139. 3-Methyladenine (3-MA) purchased from Selleck.

    Cells were treated with DMSO, TDCIPP (100 μM) only, or pretreated with 3-MA before treatment with TDCIPP (100 μM). LC3 conversion and beclin-1 and p62 expression were assessed by western blotting at 24 h

    Food Chem Toxicol, 2017, 100:183-196. 3-Methyladenine (3-MA) purchased from Selleck.

  • PC-9/ER cells were treated with CX-4945 (5 mM) for 48 h in the presence or absence of 3-MA (2 mM) and Atg7 siRNA (100 nM). Cleavage of PARP-1 and caspase-3 was shown by Western blot analysis. CT: without CX-4945; CX: CX-4945; CX+G: CX-4945 + gefitinib; CX+E: CX-4945 + erlotinib.

    PLoS One, 2014, 9(12): e114000 . 3-Methyladenine (3-MA) purchased from Selleck.

    A: The survival ratio of AML12 cells was measured after treatment with rapamycin or 3-MA; B: Western blot was used to detect the expression of LC3 and P62 in AML12 cells treated with Rapamycin or 3-MA.

    World J Gastroenterol, 2016, 22(18): 4501-4514.. 3-Methyladenine (3-MA) purchased from Selleck.

Purity & Quality Control

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Biological Activity

Description 3-Methyladenine (3-MA) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation.
Targets
Vps34 [1]
(HeLa cells)
PI3Kγ [1]
(HeLa cells)
25 μM 60 μM
In vitro

The slight preference for Vps34 prevention by 3-Methyladenine probably arises from a hydrophobic ring specific to Vps34, which encircles the 3-methyl group of 3-Methyladenine. [1] 3-Methyladenine has been reported to cause cancer cell death under both normal and starvation conditions. 3-Methyladenine could also suppress cell migration and invasion independently of its ability to inhibit autophagy, implying that 3-Methyladenine possesses functions other than autophagy suppression. 3-Methyladenine elicits caspase-dependent cell death that is independent of autophagy inhibition. Treatment with 5 mM 3-Methyladenine reduces the percentage of glucose-starved HeLa cells displaying GFP-LC3 puncta to 23%. The levels of LC3-I are increasing and the levels of LC3-II are decreasing between 12 and 48 hours in cells that are treated with 3-Methyladenine. Conversion of LC3-I to LC3-II is suppressed by 3-Methyladenine. Treatment of HeLa cells with 3-Methyladenine at 2.5 mM or 5 mM for one day does not affect cell viability, whereas treatment with 10 mM 3-Methyladenine for one day causes a 25.0% decrease in cell viability. Treatment of cells with 2.5, 5 or 10 mM 3-Methyladenine for two days causes 11.5%, 38.0% and 79.4% decrease in viability, respectively. 3-Methyladenine decreases cell viability in a time- and dose-dependent manner. 3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest. [2] Suppression of autophagy by 3-Methyladenine inhibits SU11274-induced cell death. [3] Prolonged treatment with 3-Methyladenine (up to 9 hours) induces significant LC3 I to II conversion in wild type MEFs. Prolonged treatment with 3-Methyladenine, but not wortmannin, markedly increases GFP-LC3 punctuation/aggregation. 3-Methyladenine-induced LC3 conversion and free GFP liberation are ATG7-dependent. 3-Methyladenine treatment leads to evident increase of p62 protein level. 3-Methyladenine increases the p62 level even in Atg5−/− MEFs as well as in cells with DOX-mediated deletion of ATG5. 3-Methyladenine inhibits class I and class III PI3K in different temporal patterns. 3-Methyladenine-induced LC3 I to LC3 II conversion is dramatically compromised in Tsc2−/− cells compared with wild type cells.3-Methyladenine disrupts the anti-autophagic function of mTOR complex 1. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
95D NWDLc2h[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYOzcW0> NETy[XEzcA>? M{XrVmROW09? NYPBPFBNemWmdXPld{Boem:5dHigbY5pcWKrdH;yfUBm\m[nY4Sgc4YhSkSPQx?= M2HkcVI2PzF4NU[x
A549  MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUmzcW0> M4\2fFJp M3nXNGROW09? MlvRdoVlfWOnczDndo94fGhiaX7obYJqfG:{eTDl[oZm[3Rib3[gRmROSw>? MVOyOVcyPjV4MR?=
95D NWL6S|ZzSXCxcITvd4l{KEG|c3H5 MkDXOY1O NGniVVAzcA>? M3vTfGROW09? MXLpcohq[mm2czDCSG1ENWmwZIXj[YQh[XCxcITveIlkKGOnbHyg[IVifGh? M4PET|I2PzF4NU[x
A549  MUfBdI9xfG:|aYOgRZN{[Xl? MXm1cW0> NX\w[ok6Omh? NVXqNXhlTE2VTx?= MWHpcohq[mm2czDCSG1ENWmwZIXj[YQh[XCxcITveIlkKGOnbHyg[IVifGh? M4PYPFI2PzF4NU[x
NBL-W-S  NGX0[3hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFnWd40ydU1? MUm2bC=> MVjlcohidmOnczDHRW5VNTZzIITvfIlkcXS7 M122[lI2OzJ|MkKy
NBL-W-S  NGTCXZVCeG:ydH;zbZMhSXO|YYm= M13xelFuVQ>? M1LVVFZp MWHpcoNz\WG|ZYOgZ4VtdCCjcH;weI9{cXNiaX7keYNm\CCkeTDHRW5VNTZz MXWyOVMzOzJ{Mh?=
A2780cp  NXjFU3drT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFrCd2UydU1? NIDYSZcycA>? NYrrZ5J{cW6lcnXhd4V{KGOrc4DsZZRqdi2rbnT1Z4VlKGOnbHyg[IVifGh? NFHrcpkzPTN{Mk[5OC=>
A549  NWjpR3lkTnWwY4Tpc44hSXO|YYm= M{\OZ|VuVQ>? NG[1R5AzcA>? MoGzd5VxeHKnc4Pld{BUWENiaX7keYNm\CCjY3P1cZVt[XSrb36gc4YhVEN|LVnJ NYL4PFBnOjV{OEW2Nlg>
H157 Mo\PSpVv[3Srb36gRZN{[Xl? MmnsOY1O MWiybC=> NHrtdGd{fXCycnXzd4V{KFOSQzDpcoR2[2WmIHHjZ5VufWyjdHnvckBw\iCOQ{OtTWk> MoqzNlUzQDV4Mki=
MDR M1LyVmFxd3C2b4Ppd{BCe3OjeR?= M4rJelExdU1? MlHNOog> MYDzeJJmdme2aHXud{B1cGVicH;3[ZIhd2ZiYX70bYNidmOncjDkdpVoew>? MVqyOVAyQTdyMR?=
HT-29  MUPBdI9xfG:|aYOgRZN{[Xl? MmrLNY1O MoHvOFhp MYnEUXNQ NFWze2FmdmijbnPld{Bjd3K2ZYrvcYljNWmwZIXj[YQh[2WubDD2bYFjcWyrdImgcI9{ew>? M3\wdlI1QDR{MUW4
Microglia  M3zRW2Fxd3C2b4Ppd{BCe3OjeR?= NYKwN4tSPW2P NH7OTVkzPGh? MVfk[YNz\WG|ZYOgbJlxd3irYT3pcoR2[2WmIHPlcIwh\GWjdHi= MUWyOFgyQDZyMR?=
A2780cp NXXkdVhtSXCxcITvd4l{KEG|c3H5 MViyMlVuVQ>? NYrsSVhsOWh? M{HZSoRlUDKR M4jUUYVvcGGwY3XzJINqe3CuYYTpck1qdmS3Y3XkJINmdGxiZHXheIg> M3fDXFI1QDF5OUS2
HepG2 NFHPbXRHfW6ldHnvckBCe3OjeR?= MYe1cW0> M3jUblRp Ml3tbY5kemWjc3XzJGhNKHKnbHXhd4U> Mn\vNlQ4OTN3OEe=
A549 NEX2THVCeG:ydH;zbZMhSXO|YYm= NFr1d282dU1? MWi0PIg> MUnk[YNz\WG|ZYOgeIhmKHCncnPlcpRi\2Vib3[gZ4VtdCCmZXH0bEBidmRiZYjwdoV{e2mxbjDs[ZZmdHNib3[gZ4F{eGG|ZT2zMEBD\WOuaX6tNUBidmRiTFOzMWlK NESxWGwzPDdyNkOwNy=>
U2OS NUC1fIxsSXCxcITvd4l{KEG|c3H5 NFLSZZMyOG2P NXfrVVdDOjSq MXjpcpRmdnOrZnnld{B1cGViZ4Lve5RpKGmwaHnibZRqd25ib3[geIhmKFV{T2OgZ4VtdHNiaX7keYNm\CCkeTDEc5g> MYmyOFY{QTBzMx?=
Saos-2 M{XtXWFxd3C2b4Ppd{BCe3OjeR?= NYDhO3Q6OTCvTR?= MlHHNlRp NFzZc5dqdnSnboPp[olmeyC2aHWg[5Jwf3SqIHnubIljcXSrb36gc4YhfGinIGWyU3Mh[2WubIOgbY5lfWOnZDDifUBFd3h? NWHOcFNMOjR4M{mwNVM>
A549 MVPBdI9xfG:|aYOgRZN{[Xl? M2fINFExdU1? MXi0PIg> MVXhZ4NmdGW{YYTld{B1cGVicnXkeYN1cW:wIH;mJINmdGxidnnhZoltcXS7IHnu[JVk\WRiYomgVHRZ MXyyOFYzPjd{Mh?=
HCT116  M{LuSmFxd3C2b4Ppd{BCe3OjeR?= MYi1cW0> M2XNR|I1cA>? NVTKe4pv\W6qYX7j[ZMhfGinIHHwc5B1d3OrczDpcoR2[2WmIHL5JIFxcWenbnnu MWGyOFYzPjV{Mh?=
HGC-27 NE\kfFFHfW6ldHnvckBCe3OjeR?= NHS3fHQyOG2P MU[xbC=> M1nLOYlvcGmkaYTzJJRp\SClZXzsJJZq[WKrbHn0fUBtd3O|IHL5JHJCTDByMTDvdkBOUy1{MkC2 M{fGelI1PDF4M{S5
U2OS  MnzFSpVv[3Srb36gRZN{[Xl? NEeydowxNjVxMX3N NIj5dI41QGh? NHzPSZFqdmS3Y3XzJJNidGmwb335Z4lvNWmwZIXj[YQh[2WubDD2bYFjcWyrdImgcI9{ew>? MUeyOFM2QDN2Mh?=
MG-63 M1z5UGZ2dmO2aX;uJGF{e2G7 MofKNE42NzGvTR?= NWDHWGNoPDiq MlTObY5lfWOnczDzZYxqdm:veXPpck1qdmS3Y3XkJINmdGxidnnhZoltcXS7IHzvd5M> NH\QfVIzPDN3OEO0Ni=>
MG-63 M2jSVmFxd3C2b4Ppd{BCe3OjeR?= MUSwMlUwOW2P Ml;ZN|Jp NIj6eWRmdmijbnPld{B{[Wyrbn;tfYNqdi2rbnT1Z4VlKGOnbHygZZBweHSxc3nz NH\FfIozPDN3OEO0Ni=>
MG-63 NWn5S3VXSXCxcITvd4l{KEG|c3H5 M3zpbFExdU1? NXvCUW05OTKq NUTjSIVz\W6qYX7j[ZMhTFBvaX7keYNm\CCjcH;weI9{cXN? NGHVdoczPDN3OEOwNS=>
NTUB1 M13OeWFxd3C2b4Ppd{BCe3OjeR?= MnvkOY1O NX3ud3BFOS53aB?= M3LvOZBwfGWwdHnheIV{KGOnbHXjc5hq[i2rbnT1Z4VlKGGyb4D0c5Nqew>? MWiyOFM1QTF5Nh?=
T24 NGPnW5hCeG:ydH;zbZMhSXO|YYm= M2S0[|VuVQ>? NGDTZ|AyNjWq M1\1UJBwfGWwdHnheIV{KGOnbHXjc5hq[i2rbnT1Z4VlKGGyb4D0c5Nqew>? NIrCSowzPDN2OUG3Oi=>
SGC-7901  NGKzeWhCeG:ydH;zbZMhSXO|YYm= NHe0cY8zdU1? NFy1NpMycA>? MV7pcoNz\WG|ZYOgR2EuPCCrbnT1Z4VlKGGyb4D0c5Nqew>? NIrVZVMzPDN{MUO0NC=>
SMMC-7721 MYLBdI9xfG:|aYOgRZN{[Xl? MVyycW0> NVjnRmlHOWh? NEfLc2lqdmO{ZXHz[ZMhS0FvNDDpcoR2[2WmIHHwc5B1d3Orcx?= M{jpblI1OzJzM{Sw
ECSCs  MVHBdI9xfG:|aYOgRZN{[Xl? M{ezfFExdU1? NEm5UJE1cA>? M2fOZYRm[3KnYYPld{Bz[XCjbYnjbY4ufHKnYYTl[EBieG:ydH;zbZM> M3juTVI1OzF7MUC5
MCF-7  NUL3W|BbTnWwY4Tpc44hSXO|YYm= NEmxOmEyOG2P NX7CZZN{OjSq MX;pcohq[mm2czD0bIUh[XW2b4DoZYd6KGmwZIXj[YQh[nliY3jlcY91cGW{YYD5JIRzfWe| NHvFTJYzPDNzNUW3PC=>
UOK257 Ml3PRZBweHSxc3nzJGF{e2G7 MnHnOY1O MlTFN4g> MXflcohidmOnczDwZYNtcXSjeHXsMY1m\GmjdHXkJIFxd3C2b4Ppd:Kh NEfBRZIzPDNyNU[wOC=>
ACHN-5968 MmHIRZBweHSxc3nzJGF{e2G7 MmXQOY1O NYHkPVJDO2h? NGrt[mJmdmijbnPld{Bx[WOuaYThfIVtNW2nZHnheIVlKGGyb4D0c5Nqe8Li MlriNlQ{ODV4MES=
Huh7 NUT4e|hzSXCxcITvd4l{KEG|c3H5 NGX3R3UzdU1? NUfCfZN4OTKq MljrSG1UVw>? NHj3c3hqdmirYnn0d{BCYkR6MEW1MYlv\HWlZXSgZ4VtdCCmZXH0bC=> MnnSNlQzQTd|MEC=
Hep3B NGCyflFCeG:ydH;zbZMhSXO|YYm= MVmycW0> MUKxNog> MYTEUXNQ MmnkbY5pcWKrdIOgRXpFQDB3NT3pcoR2[2WmIHPlcIwh\GWjdHi= NFTVOlUzPDJ7N{OwNC=>
RKO M3L3WGZ2dmO2aX;uJGF{e2G7 NF36VVEzdU1? MXGxbC=> MljiSG1UVw>? MX3lcohidmOnczDj[YxtKGSnYYToJIJ6KGenbHThcoFugWOrbh?= M1TRXFI1OjlzN{e3
HepG2 E47 NHPuRnRHfW6ldHnvckBCe3OjeR?= M{XuW|IvPW2P MYe0PIg> NVT5XIFOcW6lcnXhd4V{KHSqZTD0c5hq[2m2eTDv[kBCSSxiQmPPMEBidmRiQ1PsOC=> M1rBWFI1Ojd|N{O4
LoVo  NUC0NmRESXCxcITvd4l{KEG|c3H5 MmHCOY1O NYLrdZh{PDiq NYrWbJhU\W6qYX7j[ZMhTEODLXnu[JVk\WRiYYDvdJRwe2m|Lh?= NF\BdGUzPDJyMUixNi=>
WiDr NEi4codHfW6ldHnvckBCe3OjeR?= NIn0[WIyOG2P Ml7yNYg> NV7iZnU{cW6qaXLpeJMhWEOETD3pcoR2[2WmIFzDN{BKUSCneIDy[ZN{cW:w MnX4NlQyQTB2OEm=
H1299  MljFSpVv[3Srb36gRZN{[Xl? M4PFU|ExdU1? MnrVOIg> NWXYVmZXcW6lcnXhd4V{KGOrc4DsZZRqdi2rbnT1Z4VlKGOnbHyg[IVifGh? M2XOZ|I1OTd|MkC4
H460 M4DXcmZ2dmO2aX;uJGF{e2G7 NVXQbGhxOTCvTR?= MYW0bC=> MkjhbY5kemWjc3XzJINqe3CuYYTpck1qdmS3Y3XkJINmdGxiZHXheIg> NFnSRXAzPDF5M{KwPC=>
A549 NVnBUo83TnWwY4Tpc44hSXO|YYm= NGP2bmcyOG2P NV\SVoF1PGh? MljibY5pcWKrdIOgZZV1d3CqYXf5JIlv\HWlZXSgZpkhcXK{YXTpZZRqd25? NVvVSmZQOjRzNEK3N|U>
Hep3B MYHBdI9xfG:|aYOgRZN{[Xl? MV:1cW0> MV2yOIg> MYLheJRmdnWjdHXzJHRPTi4QsTDwdo91\WO2aX;uJIFo[Wmwc4Sgd4VzfW1ic4ThdpZifGmxbj3t[YRq[XSnZDDhdI9xfG:|aYO= MXGyOFA3PjZ7Mx?=
SMMC-7721 MonSRZBweHSxc3nzJGF{e2G7 MXu1cW0> NHnGToozPGh? MnXlZZR1\W63YYTld{BVVkZvzsGgdJJwfGWldHnvckBi\2GrboP0JJNmenWvIIP0ZZJ3[XSrb36tcYVlcWG2ZXSgZZBweHSxc3nz NWfxSll6OjRyNk[2PVM>
HO8910 MoTsRZBweHSxc3nzJGF{e2G7 NVHSRW9SOTCvTR?= NXjFNmp[OTKq NGLicJpmdmijbnPld{BDOTlvaX7keYNm\CCjcH;weI9{cQ>? MVmyN|k5OzZzMB?=
MCF7 NXX2[2ZyTnWwY4Tpc44hSXO|YYm= NFS0dXc2dU1? NITKfXEzPGh? NYrOUotqcW6lcnXhd4V{KEO3TzDpcoR2[2WmIHPlcIwh\GWjdHi= M1;jfVI{QTZ{NkK5
HONE-1  MV7GeY5kfGmxbjDBd5NigQ>? MXq1cW0> M1H6cVFp NF;sNI5z\XC{ZYPz[ZMhPnJvbXXkbYF1\WRiUl;TJJBzd2S3Y4Tpc44> NFixNVQzOzh7MkO1PC=>
HeLa MkDCSpVv[3Srb36gRZN{[Xl? M3LNTVExdU1? MUeybC=> NYSxNVRIe3WycILld5NmeyCOQ{OgTWkh\XiycnXzd4l{d25? M{\rZ|I{QDZ2N{O4
HepG2 MYjGeY5kfGmxbjDBd5NigQ>? NXr3OYR{OTCvTR?= MnK3NlRp NYjZSlNmcW6qaXLpeJMhe2mWSVfBVk0h[W6mIFjCV3MucW6mdXPl[EBifXSxcHjh[5k> Moq2NlM5OTdyNEC=
SH-SY5Y Mn;1SpVv[3Srb36gRZN{[Xl? MUKxcW0> NG[2[nUzPGh? MXTpcohq[mm2czD0bIUh[XW2b4DoZYd6KGmwZIXj[YQh[nliVF;DVC=> M3OzXlI{PzR|MUS4
SH-SY5Y MXzBdI9xfG:|aYOgRZN{[Xl? Mn7sOY1O MlnONYg> NX7TSJQz[WKxbHnzbIV{KGOnbHHzeJJwdCCwZYXyc5Bzd3SnY4TpeoUh\W[oZXP0 NWjMWWszOjN4MUmzPVU>
PC12  MoT4SpVv[3Srb36gRZN{[Xl? M3uze|ExdU1? MWCyOIg> MXf3ZZRmeg>? NGDZenTDqGmwaHnibZR{KGOqeX3veJJ6eHOrbj3sbYtmKHC{b4TlZZNwdWGuIHHjeIl3cXS7Lh?= M4DseFI{PjB|OUe5
OV2008 NX7RWolCSXCxcITvd4l{KEG|c3H5 MXu1cW0> M3vkbFI1cA>? Mne3Z49vfmW{dIOgSnR[PzJyIIfpeIghS0SGUDDpcpRwKGGwIHHk[Il1cX[nIHXm[oVkfCC2b4fhdoR{KGurbHzpcochd3[jcnnhckBk[W6lZYKgZ4VtdHN? MlnvNlM2QTJ{OEG=
A2780 M3LoOGFxd3C2b4Ppd{BCe3OjeR?= MoTtOY1O NEfDPXYzPGh? NGjVVIpkd264ZYL0d{BHXFl5MkCge4l1cCCFRFTQJIlvfG9iYX6gZYRlcXSrdnWg[YZn\WO2IITve4Fz\HNia3nscIlv\yCxdnHybYFvKGOjbnPldkBk\Wyucx?= MV6yN|U6OjJ6MR?=
Saos-2  NYfvO4JtSXCxcITvd4l{KEG|c3H5 M3j1bFFuVQ>? NH3pT5o6Pmh? MV7pcoNz\WG|ZYOgZ4VtdCCmZXH0bEBqdmS3Y3XkJIJ6KFCFWB?= MYOyN|U3OzF5MR?=
1321N1 NU\qcYF7S3m2b4TvfIlkcXS7IFHzd4F6 M{KzbFVuVQ>? NHu5boczPGh? M2GxVJBzd3SnY4TzJINmdGxiYXfhbY5{fCCSQ16tbY5lfWOnZDD0c5hq[2m2eR?= NWnJ[mFwOjN3MkWyOlU>
SH-SY5Y NX6wXZVMS3m2b4TvfIlkcXS7IFHzd4F6 NEjleHY2dU1? M4DsN|I1cA>? NUWz[GM4cW6lcnXhd4V{KFCFTjD0c5hq[2m2eR?= M2Ll[|I{PTJ3Mk[1
HT-29  NXS3dlZOTnWwY4Tpc44hSXO|YYm= M3fWc|FuVQ>? NYLSc|dxPDhxOU\o M2H5OIlvcGmkaYTzJGFOWEtiaX7keYNmeyCjdYTvdIhi\2mlIHPlcIwh\GWjdHi= M4TMflI{PTB6Mkey
OR6 NV3zb3VZTnWwY4Tpc44hSXO|YYm= NXfPfmE2OTCvTR?= NXnod|ZCPzKq M3;LUZN2eHC{ZYPz[ZMhUEOYIILldIxq[2G2aX;uJIFv\CCob4LtZZRqd25ib3[gZZV1d3CqYXfvd49u\XN? NES2T3QzOzN7NUi3OS=>
Hela  NXnYWoJLTnWwY4Tpc44hSXO|YYm= MoDMOY1O M{[1c|I1cA>? MXLpcohq[mm2czDzeIFzfmG2aX;uMYlv\HWlZXSgZZV1d3CqYXf5 MXmyN|M6PTZ5OR?=
MCF-7  MV3GeY5kfGmxbjDBd5NigQ>? M1XmRlVuVQ>? NVLLWIV3OjSq NHzVSIdqdmirYnn0d{B{fGG{dnH0bY9vNWmwZIXj[YQh[XW2b4DoZYd6 NVzSc5dvOjN|OUW2O|k>
HUVECs M2fYTWZ2dmO2aX;uJGF{e2G7 NVG0eoR{O22P M1;4eFI1cA>? NVvFUYo2[myxY3vzJJRp\SCycn;0[YN1cX[nIHXm[oVkfCCxZjDy[ZN3\XKjdILvcEBjgSCrbnjpZol1cW6pIHH1eI9xcGGpeR?= M{HIbVI{OzV6OUK4
T24 MlLlSpVv[3Srb36gRZN{[Xl? MoTzNVBuVQ>? NEXHWJoycA>? MVLy[YR2[2W|IITo[UBkdGWjdnHn[UBw\iCOQ{OgZYZ1\XJiYnHpZ4FtcW5idILlZZRu\W62 M130SlI{OzV2MEiw
U251MG  NX\jbo1JTnWwY4Tpc44hSXO|YYm= MVezcW0> M1THdFFp M17JR5N2eHC{ZYPz[ZMhVEN|LVnJJJBzd3SnaX6g[ZhxemW|c3nvci=> NXmwRW5kOjN|M{i2NVg>
GTL-16  NUPMfZBDSXCxcITvd4l{KEG|c3H5 M4THbFVuVQ>? MkLtNlRp MoC5doVlfWOnczDj[YxtKH[rYXLpcIl1gSCjczDjc41x[XKnZDD0c{Bk\WyuczD0doVifGWmIIfpeIghVUWWIHnubIljcXSxcoO= NY[4WnpJOjN|MUO0PVA>
T-47D M3\UdmZ2dmO2aX;uJGF{e2G7 MV2xNI1O M2rvVlJp NX7Z[25xcW6qaXLpeJMh[XW2b4DoZYd6KHC{b3Pld5Mh[W6mIHnuZ5Jm[XOnczDyZZBidXmlaX6gbY5lfWOnZDDhdI9xfG:|aYO= NGHvZWozOzNyMECyOi=>
PaCa44 MmnzRZBweHSxc3nzJGF{e2G7 M2\DblIvPW2P NGSwOHUycA>? NW\MPYFNemWmdXPld:Kh\2WwaYDpck1u\WSrYYTl[EBieG:ydH;zbZM> NG\uVW4zOzF{NEGxNi=>
MDA-MB231 NXnEfFYxTnWwY4Tpc44hSXO|YYm= NUfZbFN5PW2P MmPnNYg> MXvpcoNz\WG|ZYOgdoV{fmW{YYTyc4wudWWmaXH0[YQh[2G|cHHz[UBi[3SrdnH0bY9vKGGwZDDj[YxtKGSnYYTo NUPGZ|FnOjNyOEi4OVA>
HeLa NFfveoJCeG:ydH;zbZMhSXO|YYm= MkfkNVBuVQ>? MnLGNog> NYDBT2dN\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JINwNXS{ZXH0cYVvfCC5aYToJHBGUQ>? NIL0XWMzOzByMEGzOS=>
SK-HEP-1 MojKRZBweHSxc3nzJGF{e2G7 MknrNVBuVQ>? NWjXc3NMOWh? NWXZe3N2eHKxdHXjeJMh[WejaX7zeEBifXSxcHjh[5kh[W6mIHnu[JVk\XNiYYDvdJRwe2m|IHnuJIJ2\mGuaX6teJJm[XSnZDDj[Yxtew>? NEjvO|gzOjh3OE[0PS=>
HepG2 M4izdmFxd3C2b4Ppd{BCe3OjeR?= MoDKN41O NWfpU4tMPWh? M4fBWJJm\HWlZYOgZ4VtdCCjcH;weI9{cXNiaX7keYNm\CCkeTDRSJM> M{\qfVIzQDN4NUm1
MCF-7  NYf4[2U3TnWwY4Tpc44hSXO|YYm= M2DtZVJuVQ>? NYX0VplQOjSq NHLWNpRqdmirYnn0d{BifXSxcHjh[5khcW6mdXPl[EBjgSCWTdMg MXuyOFk4ODZ5Nh?=
MDA-MB-231 NELMWFhHfW6ldHnvckBCe3OjeR?= MWSycW0> NX\UOlBEOjSq MX7pcohq[mm2czDheZRweGijZ4mgbY5lfWOnZDDifUBVVcLi MXmyOFk4ODZ5Nh?=
MCF-7  M{nhW2Z2dmO2aX;uJGF{e2G7 Mlm4No1O M130flQ5cA>? Mor4dJJwdW:2ZYOgWG0ucW6mdXPl[EBk\WyuIHTlZZRp M2\GTlI1QTdyNke2
MDA-MB-231 NI\RbYlHfW6ldHnvckBCe3OjeR?= MmDMNo1O Mmj4OFhp NWXFbXB5eHKxbX;0[ZMhXE1vaX7keYNm\CClZXzsJIRm[XSq NV7XW4VLOjR7N{C2O|Y>
PANC-1  Mm\rRZBweHSxc3nzJGF{e2G7 Mn3uNY1O NWi2S4NkPDiq Mk\QSG1UVw>? MYnlcohidmOnczDic5J1\XqxbXniMYlv\HWlZXSgZ4VtdCC4aXHibYxqfHlibH;zdy=> M{n4WFI1QDR{MUW4
MDA-MB 231 MV7BdI9xfG:|aYOgRZN{[Xl? MljVOY1O M3TVN|AvPWh? NFmwUm1ud2S3bHH0[ZMhXG:lb33pcuKvyqCrbnT1Z4VlKGGyb4D0c5Nqew>? MorZNlQ5OzB5OEG=
Microglia  NF7TU4RCeG:ydH;zbZMhSXO|YYm= MnTKOY1O MVGyOIg> NEW1ZZZl\WO{ZXHz[ZMhcHmyb4jpZU1qdmS3Y3XkJINmdGxiZHXheIg> M{WxSlI1QDF6NkCx
A2780cp MXHBdI9xfG:|aYOgRZN{[Xl? MnTYNk42dU1? NF;VVIEycA>? Mlvt[IRJOk9? NIW3cnRmdmijbnPld{BkcXOybHH0bY4ucW6mdXPl[EBk\WyuIHTlZZRp M2jqfVI1QDF5OUS2
HepG2 NFnJepdHfW6ldHnvckBCe3OjeR?= M1TDZVVuVQ>? NXjGSII5PGh? M4W4OIlv[3KnYYPld{Bk\WyudXzhdkBt\X[nbIOgc4YhUE{EoB?= MmH4NlQ4OTN3OEe=
U2OS MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEXsbFIyOG2P NH3MfHQzPGh? NYjxZotRcW62ZX7zbYZq\XNidHjlJIdzd3e2aDDpcohq[mm2aX;uJIlv\HWlZXSgZpkhTG:6 NVqx[XI{OjR4M{mwNVM>
HCT116 MVvBdI9xfG:|aYOgRZN{[Xl? M1;xVVVuVQ>? M1XJfFI1cA>? NFzrPY5FVVOR MkPx[Y5p[W6lZYOgZZBq\2WwaX6tbY5lfWOnZDDj[YxtKGSnYYTo Mn;0NlQ3OjZ3MkK=
MCF-7  MnLDRZBweHSxc3nzJGF{e2G7 M3K2UVAvOW2P MnjKOog> NUXUR|VD\W6qYX7j[ZMhe2m{dHnuc4wucW6mdXPl[EBieG:ydH;zbZM> MVGyNlc2OTl6OR?=
PC-3  NV7Id4xMSXCxcITvd4l{KEG|c3H5 MUeycW0> M4iwU|Jp Mn7KbY5kemWjc3XzJG9TUS2rbnT1Z4VlKGOnbHyg[IVifGh? NUO4cm9tOjJ5NEW1PFA>
U251  NIru[lZCeG:ydH;zbZMhSXO|YYm= NXnudIxHPW2P MonRNlRp NHju[4RqdmO{ZXHz[ZMhWzFvaX7keYNm\CClZXzsJIRm[XSq NYnyVploOjJ3N{m3PFg>
HeLa  MVjBdI9xfG:|aYOgRZN{[Xl? MYO1cW0> NVroN4tMOjSq MWXpcoR2[2W|IHPhd5Bie2VvZHXw[Y5l\W62IHPlcIwh\GWjdHi= Mnu0NlI2PDVzMki=
A549 M1rEfGZ2dmO2aX;uJGF{e2G7 MnjnNE4ydU1? MoizNlRp M4rKdpN2eHC{ZYPz[ZMhW1VzMUK3OE1qdmS3Y3XkJINmdGxiZHXheIg> NWK3bnd{OjJ2Nk[5OlA>
pDCs NEC4PGlHfW6ldHnvckBCe3OjeR?= NETOdXMyOG2P M2OzTVAvPWh? MonFxsBz\WS3Y3XzJJRp\SCrbnT1Z5Rqd25ib3[gTWZPNc7zIHL5JJN{Wk6DNEC= MojjNlI{QTZ3OUm=
BGC-823 NFL4flZHfW6ldHnvckBCe3OjeR?= NUnnR2hUPW2P NVm3cm5GOmh? MVvpcohq[mm2czD0bIUhemG2ZTDv[kBifXSxcHjh[4lkKGOnbHzz MlzkNlI{OjJzNUK=
U937 M1nuRWZ2dmO2aX;uJGF{e2G7 NFu0T5QzdU1? NGHzWYUyOmh? MoDk[IVkemWjc3XzJJRp\SCjdYTvdIhi\3licnH0bY/DqA>? MWKyNlE2PTF3MB?=
Marc-145 Ml[wSpVv[3Srb36gRZN{[Xl? NFK5bog2dU1? NWK5N3RqOTJxMkSvN|Zp MWLy[YR2[2W|IITo[UBRWlKVVjD0bZRmenNiYX7kJJRp\SCycn;0[YlvKGW6cILld5Nqd25? MUGyNlEyQTlyMB?=
HBx  MnrtRZBweHSxc3nzJGF{e2G7 NGDDcYsyOG2P NUe1O|NxPDiq NFrRXFNFVVOR NFO2ZZJqdmO{ZXHz[ZMh[2WubDDk[YF1cA>? MXiyNlAzODB5OB?=
MCF-7 M1;rXmZ2dmO2aX;uJGF{e2G7 NGfHXG4yOG2P NU\2[YVvPDiq M4DIWYJtd2OtczDheZRweGijZ4mgbY5lfWOnZDDifUBjd3K2ZYrvcYlj NWW2dHBPOjF7M{G5N|c>
RMPI8226  MkC1SpVv[3Srb36gRZN{[Xl? M13ZWFVuVQ>? NIPoT|YycA>? NGnoe5B{fXCycnXzd4V{KHSqZTDs[ZZmdCCxZjDheZRweGijZ4mgeY5l\XJiboX0dolmdnRiZHXwcIV1cW:w M3TEbFIyQTF3NkKw
PC12/TetOn NH\6eZhHfW6ldHnvckBCe3OjeR?= NVjweoxDOC5zL{HtUS=> NWDIcFJxOTiq NYLQc3hPdGWjZIOgeI8h|rFvc4nuLHdVMSCjY3P1cZVt[XSrb36sJJRwgGmlaYT5MEBidmRib3zp[49u\XJiZn;ycYF1cW:wwrC= M1PUS|IyQTB4NkW5
HeLa  NGi0O29EgXSxdH;4bYNqfHliQYPzZZk> MX2ycW0> Mn;LNlRp NF:zNXhqdmirYnn0[ZMhfGinIHP5eI91d3irY3n0fUBw\iC|aXzpZolvcW5idH:gTIVN[SClZXzsd{4> NWXXWIpYOjF6N{WzPFU>
Jurkat NW\Hc|JzTnWwY4Tpc44hSXO|YYm= MYGxNI1O NI\YeVAycA>? MnrX[IVkemWjc3XzJJRp\SCneIDy[ZN{cW:wIH;mJGxEOy2LSTDhcoQhfGinIH\vdo1ifGmxbjDv[kBifXSxcHjh[49{d22ncx?= MXyyNVg3PDB|Nx?=
K562 MVjGeY5kfGmxbjDBd5NigQ>? MWKxNI1O MmfVNYg> NGPtPZRl\WO{ZXHz[ZMhfGinIHX4dJJme3Orb36gc4YhVEN|LVnJJIFv\CC2aHWg[o9zdWG2aX;uJI9nKGG3dH;wbIFod3OxbXXz NWrHe444OjF6NkSwN|c>
Nara-H MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHHaN2U2dU1? MYS0PIg> MVvlcohidmOnczD0[Y1{cXKxbHnteZNu\WSrYYTl[EB{fXCycnXzd4lwdiCxZjDOZZJiNUhiY3XscEBxem:uaX\ldoF1cW:w NUTCWJV6OjF6MEWwN|M>
HUVECs M1j3bWZ2dmO2aX;uJGF{e2G7 M2nBUlExdU1? NFm2XIExNjWq MXLk[YNz\WG|ZYOgeIhmKEGJRT3CV2FqdmS3Y3XkJIF2fG:yaHHnfUBt\X[n MkPxNlE1Pjh3OUK=
HepG2 Mn[xRZBweHSxc3nzJGF{e2G7 NWP2eZNuOm2P NIL2SGQycA>? M3Oz[YVvcGGwY3XzJJJi\GmjdHnvck1qdmS3Y3XkJINmdGxiZHXheIg> M{X1[lIyPDV|Nkmx

... Click to View More Cell Line Experimental Data

In vivo 3-Methyladenine blocks autophagy through its effect on class III phosphatidylinositol 3-kinase (PI3K). 3-Methyladenine treatment does not alter the degree of hemorrhage compared with the subarachnoid hemorrhage (SAH) group. 3-Methyladenine pretreatment significantly aggravates neurological symptoms when compared with the SAH + vehicle group. Autophagy is decreased when 3-Methyladenine treatment is applied. Conversely, cleaved caspase-3 is markedly up-regulated in the SAH + 3-Methyladenine group. In line with the up-regulation of cleaved caspase-3 expression, the number of TUNEL-positive cells in the right cortex is significantly increased in the SAH + 3-Methyladenine group compared with the SAH + vehicle group. [5]

Protocol

Kinase Assay:[4]
+ Expand

Protein degradation assay:

HeLa cells are radiolabeled for 24 hours with 0.05 mCi/mL l-[U- 14C]valine. At the end of the labeling period, cells are rinsed three times with PBS. Cells are incubated for the designated times in either full medium or EBSS with or without the presence of 10 mM 3-Methyladenine.
Cell Research:[2]
+ Expand
  • Cell lines: HeLa cell line
  • Concentrations: 1-10 mM
  • Incubation Time: 24, 48 or 72 hours
  • Method: Cell (such as HeLa cell) viability is determined by a trypan blue exclusion assay. Briefly, after treated with 3-Methyladenine, both adherent and floating cells are collected and suspended in phosphate buffered saline (PBS, pH 7.4) at a final density of 1-2 × 106/mL. An equal volume of 0.4% trypan blue solution (w/v, in PBS) is added to the cell suspension and mixed thoroughly. After incubation at room temperature for 3 min, cell counting is performed using a hemacytometer.
    (Only for Reference)
Animal Research:[5]
+ Expand
  • Animal Models: Adult male Sprague–Dawley rats weighing 300-350 g
  • Formulation: Saline
  • Dosages: 400 nM
  • Administration: Intracerebral ventricular
    (Only for Reference)

Solubility (25°C)

In vitro Water 10 mg/mL warmed (67.04 mM)
Ethanol 4 mg/mL (26.81 mM)
DMSO 3 mg/mL warmed (20.11 mM)
In vivo Add solvents individually and in order:
water
10mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 149.15
Formula

C6H7N5

CAS No. 5142-23-4
Storage powder
in solvent
Synonyms NSC 66389

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

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Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID