3-Methyladenine (3-MA)

Catalog No.S2767 Synonyms: NSC 66389

3-Methyladenine (3-MA) Chemical Structure

Molecular Weight(MW): 149.15

3-Methyladenine (3-MA) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation.

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4 Customer Reviews

  • MDC-labeled vacuoles were induced by AZD2014 and inhibited by autophagy inhibitor (3-MA). SMMC-7721 cells were treated with AZD2014 or rapamycin at concentrations of 100 and 600 nM, respectively, for 48 hours in the presence or absence of 3-MA, and then stained with MDC. Cells were immediately observed under a confocal microscope. Cells in the control group were treated with DMSO. bars, 20 μm.

    Am J Cancer Res, 2015, 5(1): 125-139. 3-Methyladenine (3-MA) purchased from Selleck.

    Cells were treated with DMSO, TDCIPP (100 μM) only, or pretreated with 3-MA before treatment with TDCIPP (100 μM). LC3 conversion and beclin-1 and p62 expression were assessed by western blotting at 24 h

    Food Chem Toxicol, 2017, 100:183-196. 3-Methyladenine (3-MA) purchased from Selleck.

  • PC-9/ER cells were treated with CX-4945 (5 mM) for 48 h in the presence or absence of 3-MA (2 mM) and Atg7 siRNA (100 nM). Cleavage of PARP-1 and caspase-3 was shown by Western blot analysis. CT: without CX-4945; CX: CX-4945; CX+G: CX-4945 + gefitinib; CX+E: CX-4945 + erlotinib.

    PLoS One, 2014, 9(12): e114000 . 3-Methyladenine (3-MA) purchased from Selleck.

    A: The survival ratio of AML12 cells was measured after treatment with rapamycin or 3-MA; B: Western blot was used to detect the expression of LC3 and P62 in AML12 cells treated with Rapamycin or 3-MA.

    World J Gastroenterol, 2016, 22(18): 4501-4514.. 3-Methyladenine (3-MA) purchased from Selleck.

Purity & Quality Control

Choose Selective PI3K Inhibitors

Biological Activity

Description 3-Methyladenine (3-MA) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation.
Targets
Vps34 [1]
(HeLa cells)
PI3Kγ [1]
(HeLa cells)
25 μM 60 μM
In vitro

The slight preference for Vps34 prevention by 3-Methyladenine probably arises from a hydrophobic ring specific to Vps34, which encircles the 3-methyl group of 3-Methyladenine. [1] 3-Methyladenine has been reported to cause cancer cell death under both normal and starvation conditions. 3-Methyladenine could also suppress cell migration and invasion independently of its ability to inhibit autophagy, implying that 3-Methyladenine possesses functions other than autophagy suppression. 3-Methyladenine elicits caspase-dependent cell death that is independent of autophagy inhibition. Treatment with 5 mM 3-Methyladenine reduces the percentage of glucose-starved HeLa cells displaying GFP-LC3 puncta to 23%. The levels of LC3-I are increasing and the levels of LC3-II are decreasing between 12 and 48 hours in cells that are treated with 3-Methyladenine. Conversion of LC3-I to LC3-II is suppressed by 3-Methyladenine. Treatment of HeLa cells with 3-Methyladenine at 2.5 mM or 5 mM for one day does not affect cell viability, whereas treatment with 10 mM 3-Methyladenine for one day causes a 25.0% decrease in cell viability. Treatment of cells with 2.5, 5 or 10 mM 3-Methyladenine for two days causes 11.5%, 38.0% and 79.4% decrease in viability, respectively. 3-Methyladenine decreases cell viability in a time- and dose-dependent manner. 3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest. [2] Suppression of autophagy by 3-Methyladenine inhibits SU11274-induced cell death. [3] Prolonged treatment with 3-Methyladenine (up to 9 hours) induces significant LC3 I to II conversion in wild type MEFs. Prolonged treatment with 3-Methyladenine, but not wortmannin, markedly increases GFP-LC3 punctuation/aggregation. 3-Methyladenine-induced LC3 conversion and free GFP liberation are ATG7-dependent. 3-Methyladenine treatment leads to evident increase of p62 protein level. 3-Methyladenine increases the p62 level even in Atg5−/− MEFs as well as in cells with DOX-mediated deletion of ATG5. 3-Methyladenine inhibits class I and class III PI3K in different temporal patterns. 3-Methyladenine-induced LC3 I to LC3 II conversion is dramatically compromised in Tsc2−/− cells compared with wild type cells.3-Methyladenine disrupts the anti-autophagic function of mTOR complex 1. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
95D NV63R29oT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{jL[FNuVQ>? NHThT48zcA>? M2ns[2ROW09? MX3y[YR2[2W|IHfyc5d1cCCrbnjpZol1d3K7IHXm[oVkfCCxZjDCSG1E NUXySHk1OjV5MU[1OlE>
A549  MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYKzcW0> MXiybC=> NHT6c4ZFVVOR M{TCSZJm\HWlZYOg[5Jwf3SqIHnubIljcXSxcomg[YZn\WO2IH;mJGJFVUN? NUPtSFlzOjV5MU[1OlE>
95D MlPKRZBweHSxc3nzJGF{e2G7 NUPsT4VPPW2P NIS5OWczcA>? Mlr5SG1UVw>? MmrMbY5pcWKrdIOgRmROSy2rbnT1Z4VlKGGyb4D0c5Rq[yClZXzsJIRm[XSq M{jtcVI2PzF4NU[x
A549  NV:1XG9kSXCxcITvd4l{KEG|c3H5 NFHzR4o2dU1? NWXtSVl3Omh? MmHKSG1UVw>? MVrpcohq[mm2czDCSG1ENWmwZIXj[YQh[XCxcITveIlkKGOnbHyg[IVifGh? MW[yOVcyPjV4MR?=
NBL-W-S  NHf1b4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4C4UlFuVQ>? M{DWZ|Zp NGTvNXhmdmijbnPld{BISU6WLU[xJJRwgGmlaYT5 NWnnem9LOjV|MkOyNlI>
NBL-W-S  NHPMUFJCeG:ydH;zbZMhSXO|YYm= NWH3elViOW2P M4m4VFZp MVPpcoNz\WG|ZYOgZ4VtdCCjcH;weI9{cXNiaX7keYNm\CCkeTDHRW5VNTZz NEfRV5ozPTN{M{KyNi=>
A2780cp  MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHnEdJAydU1? NWH0Z5dJOWh? MnrBbY5kemWjc3XzJINqe3CuYYTpck1qdmS3Y3XkJINmdGxiZHXheIg> M1zjb|I2OzJ{Nkm0
A549  Ml3DSpVv[3Srb36gRZN{[Xl? NFy2OXY2dU1? NEXlSWMzcA>? Mn\Cd5VxeHKnc4Pld{BUWENiaX7keYNm\CCjY3P1cZVt[XSrb36gc4YhVEN|LVnJ M{G3V|I2Ojh3NkK4
H157 MmrUSpVv[3Srb36gRZN{[Xl? NUfm[m95PW2P NH7zW5MzcA>? MmLPd5VxeHKnc4Pld{BUWENiaX7keYNm\CCjY3P1cZVt[XSrb36gc4YhVEN|LVnJ NYfTOIM6OjV{OEW2Nlg>
MDR M1HDemFxd3C2b4Ppd{BCe3OjeR?= Mlv5NVBuVQ>? MkTFOog> Ml21d5Rz\W6pdHjlcpMhfGinIIDve4VzKG:oIHHueIlk[W6lZYKg[JJ2\3N? NIntN4gzPTBzOUewNS=>
HT-29  MkjhRZBweHSxc3nzJGF{e2G7 NGrrSYUydU1? M3ztSVQ5cA>? MXjEUXNQ MUHlcohidmOnczDic5J1\XqxbXniMYlv\HWlZXSgZ4VtdCC4aXHibYxqfHlibH;zdy=> M2\5fVI1QDR{MUW4
Microglia  MWTBdI9xfG:|aYOgRZN{[Xl? NW\oWIt{PW2P MmTDNlRp M{TiUIRm[3KnYYPld{BpgXCxeHnhMYlv\HWlZXSgZ4VtdCCmZXH0bC=> MXKyOFgyQDZyMR?=
A2780cp NVXucJJXSXCxcITvd4l{KEG|c3H5 MV[yMlVuVQ>? M4XDfFFp Mln1[IRJOk9? MULlcohidmOnczDjbZNxdGG2aX6tbY5lfWOnZDDj[YxtKGSnYYTo Ml7SNlQ5OTd7NE[=
HepG2 MlTkSpVv[3Srb36gRZN{[Xl? NX62WotIPW2P M3HuR|Rp MmDUbY5kemWjc3XzJGhNKHKnbHXhd4U> MVeyOFcyOzV6Nx?=
A549 NEjjbGlCeG:ydH;zbZMhSXO|YYm= Ml7xOY1O NX7TfnBIPDiq Mlr4[IVkemWjc3XzJJRp\SCyZYLj[Y51[WenIH;mJINmdGxiZHXheIgh[W6mIHX4dJJme3Orb36gcIV3\Wy|IH;mJINie3Cjc3WtN{whSmWlbHnuMVEh[W6mIFzDN{1KUQ>? M4LCNlI1PzB4M{Cz
U2OS MUTBdI9xfG:|aYOgRZN{[Xl? NFfyW4QyOG2P MYqyOIg> NVXpbpF5cW62ZX7zbYZq\XNidHjlJIdzd3e2aDDpcohq[mm2aX;uJI9nKHSqZTDVNm9UKGOnbHzzJIlv\HWlZXSgZpkhTG:6 MUCyOFY{QTBzMx?=
Saos-2 Ml;HRZBweHSxc3nzJGF{e2G7 Ml;sNVBuVQ>? NYD3fJBmOjSq NFPPdnpqdnSnboPp[olmeyC2aHWg[5Jwf3SqIHnubIljcXSrb36gc4YhfGinIGWyU3Mh[2WubIOgbY5lfWOnZDDifUBFd3h? MXuyOFY{QTBzMx?=
A549 MYXBdI9xfG:|aYOgRZN{[Xl? M3v5OVExdU1? NWKyOGQ2PDiq M4npSoFk[2WuZYLheIV{KHSqZTDy[YR2[3Srb36gc4Yh[2WubDD2bYFjcWyrdImgbY5lfWOnZDDifUBRXFh? NUnuVXU6OjR4Mk[3NlI>
HCT116  NYX6VGdOSXCxcITvd4l{KEG|c3H5 M1TuelVuVQ>? M2j1dVI1cA>? M{nOVoVvcGGwY3XzJJRp\SCjcH;weI9{cXNiaX7keYNm\CCkeTDhdIlo\W6rbh?= NI\FOFUzPDZ{NkWyNi=>
HGC-27 MV;GeY5kfGmxbjDBd5NigQ>? NIjPcYIyOG2P MWGxbC=> NGfreo5qdmirYnn0d{B1cGViY3XscEB3cWGkaXzpeJkhdG:|czDifUBTSURyMEGgc5IhVUtvMkKwOi=> M2q1fVI1PDF4M{S5
U2OS  Ml;MSpVv[3Srb36gRZN{[Xl? NVyzSXNxOC53L{HtUS=> NFPCOXM1QGh? MWHpcoR2[2W|IIPhcIlvd227Y3nuMYlv\HWlZXSgZ4VtdCC4aXHibYxqfHlibH;zdy=> M2f1NVI1OzV6M{Sy
MG-63 NX:yOnFbTnWwY4Tpc44hSXO|YYm= Ml3jNE42NzGvTR?= NXTxWXJ3PDiq M4P4[4lv\HWlZYOgd4FtcW6xbYnjbY4ucW6mdXPl[EBk\WyuII\pZYJqdGm2eTDsc5N{ NV;iXWZHOjR|NUizOFI>
MG-63 MmLqRZBweHSxc3nzJGF{e2G7 NGP6OZUxNjVxMX3N NGnhOIg{Omh? NHjSTmVmdmijbnPld{B{[Wyrbn;tfYNqdi2rbnT1Z4VlKGOnbHygZZBweHSxc3nz MYeyOFM2QDN2Mh?=
MG-63 MkXORZBweHSxc3nzJGF{e2G7 NXXxXnZSOTCvTR?= NFX1elcyOmh? NYDkb4li\W6qYX7j[ZMhTFBvaX7keYNm\CCjcH;weI9{cXN? M4nSelI1OzV6M{Cx
NTUB1 NF3I[4dCeG:ydH;zbZMhSXO|YYm= Mnv3OY1O NYDleoZrOS53aB?= MXnwc5RmdnSrYYTld{Bk\WynY3;4bYIucW6mdXPl[EBieG:ydH;zbZM> MWGyOFM1QTF5Nh?=
T24 Mn;ERZBweHSxc3nzJGF{e2G7 MVe1cW0> MVmxMlVp M{S3NZBwfGWwdHnheIV{KGOnbHXjc5hq[i2rbnT1Z4VlKGGyb4D0c5Nqew>? NFTBeJczPDN2OUG3Oi=>
SGC-7901  NX36WWw6SXCxcITvd4l{KEG|c3H5 MmrNNo1O M3HuNlFp MUHpcoNz\WG|ZYOgR2EuPCCrbnT1Z4VlKGGyb4D0c5Nqew>? NH7vOFAzPDN{MUO0NC=>
SMMC-7721 NXv6Z4U6SXCxcITvd4l{KEG|c3H5 MmHzNo1O M{X5OVFp M3P0dYlv[3KnYYPld{BESS12IHnu[JVk\WRiYYDvdJRwe2m| NFvZW|YzPDN{MUO0NC=>
ECSCs  M4LBbmFxd3C2b4Ppd{BCe3OjeR?= MV2xNI1O NGPteYM1cA>? MUXk[YNz\WG|ZYOgdoFx[W27Y3nuMZRz\WG2ZXSgZZBweHSxc3nz NVnRSGRuOjR|MUmxNFk>
MCF-7  NYG3fFFWTnWwY4Tpc44hSXO|YYm= NVrGe5IyOTCvTR?= M4GxZVI1cA>? NILGT5JqdmirYnn0d{B1cGViYYX0c5Bp[We7IHnu[JVk\WRiYomgZ4hmdW:2aHXyZZB6KGS{dXfz MX6yOFMyPTV5OB?=
UOK257 MnPRRZBweHSxc3nzJGF{e2G7 MWi1cW0> NHPhPVM{cA>? NUHzdYZv\W6qYX7j[ZMheGGlbHn0ZZhmdC2vZXTpZZRm\CCjcH;weI9{cXQEoB?= MorQNlQ{ODV4MES=
ACHN-5968 MnnlRZBweHSxc3nzJGF{e2G7 NYO1WGoyPW2P NFjyXpY{cA>? MmPw[Y5p[W6lZYOgdIFkdGm2YYjlcE1u\WSrYYTl[EBieG:ydH;zbZPDqA>? NYrvZ41FOjR|MEW2NFQ>
Huh7 M1vBOGFxd3C2b4Ppd{BCe3OjeR?= M2ewSFJuVQ>? M1XvcFEzcA>? M1mxRmROW09? MnPSbY5pcWKrdIOgRXpFQDB3NT3pcoR2[2WmIHPlcIwh\GWjdHi= MlruNlQzQTd|MEC=
Hep3B MkC2RZBweHSxc3nzJGF{e2G7 M4\4SVJuVQ>? MUKxNog> NYTmSpN7TE2VTx?= NYrNcYFNcW6qaXLpeJMhSVqGOEC1OU1qdmS3Y3XkJINmdGxiZHXheIg> NFT0fYYzPDJ7N{OwNC=>
RKO NInjNZFHfW6ldHnvckBCe3OjeR?= Ml3QNo1O MYCxbC=> M37DZ2ROW09? MX;lcohidmOnczDj[YxtKGSnYYToJIJ6KGenbHThcoFugWOrbh?= MoiwNlQzQTF5N{e=
HepG2 E47 MkPPSpVv[3Srb36gRZN{[Xl? M1\3ZlIvPW2P MV20PIg> M4O2bIlv[3KnYYPld{B1cGVidH;4bYNqfHlib3[gRWEtKEKVTzygZY5lKEOFbES= MXmyOFI4Ozd|OB?=
LoVo  NHHyOXZCeG:ydH;zbZMhSXO|YYm= MmHkOY1O NWHp[3lHPDiq MXHlcohidmOnczDER2EucW6mdXPl[EBieG:ydH;zbZMv MXmyOFIxOThzMh?=
WiDr NYroboJSTnWwY4Tpc44hSXO|YYm= M3fvZlExdU1? NYHBRmFuOWh? M1O4VIlvcGmkaYTzJHBESkxvaX7keYNm\CCOQ{OgTWkh\XiycnXzd4lwdg>? M2HHW|I1OTlyNEi5
H1299  MXjGeY5kfGmxbjDBd5NigQ>? MWqxNI1O NIL6W|c1cA>? NF\wdlFqdmO{ZXHz[ZMh[2m|cHzheIlvNWmwZIXj[YQh[2WubDDk[YF1cA>? NEG5[XAzPDF5M{KwPC=>
H460 NXXQfIxHTnWwY4Tpc44hSXO|YYm= NU\aU3lrOTCvTR?= NGDUNHA1cA>? NFzrTGdqdmO{ZXHz[ZMh[2m|cHzheIlvNWmwZIXj[YQh[2WubDDk[YF1cA>? MoHkNlQyPzN{MEi=
A549 NGXPd5JHfW6ldHnvckBCe3OjeR?= M4n0elExdU1? MXW0bC=> MU\pcohq[mm2czDheZRweGijZ4mgbY5lfWOnZDDifUBqenKjZHnheIlwdg>? NGfPZ|UzPDF2MkezOS=>
Hep3B MX;BdI9xfG:|aYOgRZN{[Xl? MYi1cW0> NYXHPWFiOjSq M4qyeoF1fGWwdXH0[ZMhXE6ILd8xJJBzd3SnY4Tpc44h[WejaX7zeEB{\XK3bTDzeIFzfmG2aX;uMY1m\GmjdHXkJIFxd3C2b4Ppdy=> NGHKWYgzPDB4Nk[5Ny=>
SMMC-7721 MoXjRZBweHSxc3nzJGF{e2G7 MXW1cW0> NIqxe2wzPGh? NY\LbVY{[XS2ZX71ZZRmeyCWTl[t{tEheHKxdHXjeIlwdiCjZ3HpcpN1KHOncoXtJJN1[XK4YYTpc44udWWmaXH0[YQh[XCxcITvd4l{ MlrtNlQxPjZ4OUO=
HO8910 MUXBdI9xfG:|aYOgRZN{[Xl? NGTqS48yOG2P MlnrNVJp NIXuT4tmdmijbnPld{BDOTlvaX7keYNm\CCjcH;weI9{cQ>? NF;jTYkzOzl6M{[xNC=>
MCF7 M4PYc2Z2dmO2aX;uJGF{e2G7 M1PQUVVuVQ>? M{HRcFI1cA>? M17yNIlv[3KnYYPld{BEfU9iaX7keYNm\CClZXzsJIRm[XSq MmfrNlM6PjJ4Mkm=
HONE-1  NYHBb2JmTnWwY4Tpc44hSXO|YYm= M1nITFVuVQ>? NYnOVYdROWh? MVny[ZBz\XO|ZYOgOpIudWWmaXH0[YQhWk:VIIDyc4R2[3Srb36= MVuyN|g6OjN3OB?=
HeLa M32x[2Z2dmO2aX;uJGF{e2G7 NIfRSm0yOG2P NWDy[ZFUOmh? MnjKd5VxeHKnc4Pld{BNSzNiSVmg[ZhxemW|c3nzc44> M2WxNFI{QDZ2N{O4
HepG2 M1rMcWZ2dmO2aX;uJGF{e2G7 MnrONVBuVQ>? MVKyOIg> NXXWb|JYcW6qaXLpeJMhe2mWSVfBVk0h[W6mIFjCV3MucW6mdXPl[EBifXSxcHjh[5k> MYCyN|gyPzB2MB?=
SH-SY5Y MXTGeY5kfGmxbjDBd5NigQ>? MnLYNY1O Mke2NlRp M{\2ZYlvcGmkaYTzJJRp\SCjdYTvdIhi\3liaX7keYNm\CCkeTDUU2NR M1PZNFI{PzR|MUS4
SH-SY5Y M2XKbmFxd3C2b4Ppd{BCe3OjeR?= NHXme2k2dU1? M{XnNFFp NH7LR2pi[m:uaYPo[ZMh[2WuYYP0do9tKG6ndYLvdJJwfGWldHn2[UBm\m[nY4S= M4CyVFI{PjF7M{m1
PC12  MorkSpVv[3Srb36gRZN{[Xl? MV6xNI1O M1nITVI1cA>? NEG0NIp4[XSnch?= M{XuZeKhcW6qaXLpeJMh[2i7bX;0dplxe2mwLXzpb4UheHKxdHXhd49u[WxiYXP0bZZqfHlw MlnsNlM3ODN7N{m=
OV2008 NVnnT5hHSXCxcITvd4l{KEG|c3H5 MljtOY1O NXrk[mE1OjSq MkSyZ49vfmW{dIOgSnR[PzJyIIfpeIghS0SGUDDpcpRwKGGwIHHk[Il1cX[nIHXm[oVkfCC2b4fhdoR{KGurbHzpcochd3[jcnnhckBk[W6lZYKgZ4VtdHN? MoXINlM2QTJ{OEG=
A2780 MnLtRZBweHSxc3nzJGF{e2G7 MUm1cW0> Mli0NlRp M3G4doNwdn[ncoTzJGZVYTd{MDD3bZRpKEOGRGCgbY51dyCjbjDh[IRqfGm4ZTDl[oZm[3RidH;3ZZJleyCtaXzsbY5oKG:4YYLpZY4h[2GwY3XyJINmdGy| NF62NIUzOzV7MkK4NS=>
Saos-2  NX;vZldESXCxcITvd4l{KEG|c3H5 MoriNY1O MVu5Oog> NGLtVHhqdmO{ZXHz[ZMh[2WubDDk[YF1cCCrbnT1Z4VlKGK7IGDDXC=> M2ntRVI{PTZ|MUex
1321N1 MnHBR5l1d3SxeHnjbZR6KEG|c3H5 NFO1em02dU1? MX6yOIg> M1rxe5Bzd3SnY4TzJINmdGxiYXfhbY5{fCCSQ16tbY5lfWOnZDD0c5hq[2m2eR?= NXLmNXk6OjN3MkWyOlU>
SH-SY5Y NIrwZlNEgXSxdH;4bYNqfHliQYPzZZk> M2PSblVuVQ>? NGK0doMzPGh? MXHpcoNz\WG|ZYOgVGNPKHSxeHnjbZR6 M3TPRlI{PTJ3Mk[1
HT-29  M1jIUGZ2dmO2aX;uJGF{e2G7 NHLCcmIydU1? MmfDOFgwQT[q MmPKbY5pcWKrdIOgRW1RUyCrbnT1Z4V{KGG3dH;wbIFocWNiY3XscEBl\WG2aB?= MWiyN|UxQDJ5Mh?=
OR6 MlvLSpVv[3Srb36gRZN{[Xl? NGjNUmkyOG2P MkW1O|Jp MVXzeZBxemW|c3XzJGhEXiC{ZYDsbYNifGmxbjDhcoQh\m:{bXH0bY9vKG:oIHH1eI9xcGGpb4PvcYV{ Mkj5NlM{QTV6N{W=
Hela  MWnGeY5kfGmxbjDBd5NigQ>? NV3VXmRHPW2P MVyyOIg> MnfObY5pcWKrdIOgd5Rien[jdHnvck1qdmS3Y3XkJIF2fG:yaHHnfS=> NH6xU5MzOzN7NU[3PS=>
MCF-7  NECwU|hHfW6ldHnvckBCe3OjeR?= NVzCNok3PW2P NIXKU40zPGh? MVvpcohq[mm2czDzeIFzfmG2aX;uMYlv\HWlZXSgZZV1d3CqYXf5 NI\nVpMzOzN7NU[3PS=>
HUVECs MU\GeY5kfGmxbjDBd5NigQ>? NHrzdnc{dU1? NFvOcoIzPGh? NH3KRWpjdG:la4OgeIhmKHC{b4TlZ5RqfmViZX\m[YN1KG:oIILld5ZmemG2cn;sJIJ6KGmwaHnibZRqdmdiYYX0c5Bp[We7 MWSyN|M2QDl{OB?=
T24 MoLkSpVv[3Srb36gRZN{[Xl? NUXNWWtpOTCvTR?= M2m3eFFp MoLPdoVlfWOnczD0bIUh[2ynYY\h[4Uhd2ZiTFOzJIFnfGW{IHLhbYNidGmwIITy[YF1dWWwdB?= NYrGZ2VuOjN|NUSwPFA>
U251MG  M2\lbmZ2dmO2aX;uJGF{e2G7 NUDqd3N6O22P NIfCOWQycA>? NYrQS3JIe3WycILld5NmeyCOQ{OtTWkheHKxdHXpckBmgHC{ZYPzbY9v NXPQO2JyOjN|M{i2NVg>
GTL-16  M4fhPGFxd3C2b4Ppd{BCe3OjeR?= NHi5c3Q2dU1? MlrONlRp MkfMdoVlfWOnczDj[YxtKH[rYXLpcIl1gSCjczDjc41x[XKnZDD0c{Bk\WyuczD0doVifGWmIIfpeIghVUWWIHnubIljcXSxcoO= NV76PXhqOjN|MUO0PVA>
T-47D MWXGeY5kfGmxbjDBd5NigQ>? MlzYNVBuVQ>? MVKybC=> NHH5d4RqdmirYnn0d{BifXSxcHjh[5kheHKxY3Xzd{BidmRiaX7jdoVie2W|IILhdIFugWOrbjDpcoR2[2WmIHHwc5B1d3Orcx?= NXrkS4diOjN|MECwNlY>
PaCa44 NFK0UHlCeG:ydH;zbZMhSXO|YYm= MVOyMlVuVQ>? M1zaN|Fp MYTy[YR2[2W|wrDn[Y5qeGmwLX3l[IlifGWmIHHwc5B1d3Orcx?= NWTxeIhqOjNzMkSxNVI>
MDA-MB231 MXfGeY5kfGmxbjDBd5NigQ>? M{THWlVuVQ>? NEXwPIEycA>? MoLIbY5kemWjc3XzJJJme3[ncnH0do9tNW2nZHnheIVlKGOjc4Dhd4Uh[WO2aY\heIlwdiCjbnSgZ4VtdCCmZXH0bC=> M1\EbVI{ODh6OEWw
HeLa MUjBdI9xfG:|aYOgRZN{[Xl? NGL5[3EyOG2P NUHxeVFsOmh? MV;k[YNz\WG|ZYOgZ4VtdCC4aXHibYxqfHliY3:teJJm[XSvZX70JJdqfGhiUFXJ MnzxNlMxODBzM{W=
SK-HEP-1 MkfoRZBweHSxc3nzJGF{e2G7 M3O0NlExdU1? NGm3VVcycA>? MVHwdo91\WO2czDh[4FqdnO2IHH1eI9xcGGpeTDhcoQhcW6mdXPld{BieG:ydH;zbZMhcW5iYoXmZYxqdi22cnXheIVlKGOnbHzz NXTEfopLOjJ6NUi2OFk>
HepG2 M3;KS2Fxd3C2b4Ppd{BCe3OjeR?= NG\CNng{dU1? MYe1bC=> MmLDdoVlfWOnczDj[YxtKGGyb4D0c5NqeyCrbnT1Z4VlKGK7IGHEdy=> M4jUO|IzQDN4NUm1
MCF-7  NXvhc4NGTnWwY4Tpc44hSXO|YYm= M3rTUlJuVQ>? M2LwR|I1cA>? MonrbY5pcWKrdIOgZZV1d3CqYXf5JIlv\HWlZXSgZpkhXE4EoB?= MnmyNlQ6PzB4N{[=
MDA-MB-231 NEizZ|FHfW6ldHnvckBCe3OjeR?= NYjv[FlHOm2P MYOyOIg> NHjsdmpqdmirYnn0d{BifXSxcHjh[5khcW6mdXPl[EBjgSCWTdMg NGDpZpAzPDl5ME[3Oi=>
MCF-7  MYTGeY5kfGmxbjDBd5NigQ>? MV6ycW0> MYG0PIg> NWrjRpZweHKxbX;0[ZMhXE1vaX7keYNm\CClZXzsJIRm[XSq NI\GUY0zPDl5ME[3Oi=>
MDA-MB-231 M4HLdGZ2dmO2aX;uJGF{e2G7 NHzjSYMzdU1? MojPOFhp MoXxdJJwdW:2ZYOgWG0ucW6mdXPl[EBk\WyuIHTlZZRp MXuyOFk4ODZ5Nh?=
PANC-1  NYjmd|EySXCxcITvd4l{KEG|c3H5 NXXJUoV3OW2P MofoOFhp MY\EUXNQ NELWN4pmdmijbnPld{Bjd3K2ZYrvcYljNWmwZIXj[YQh[2WubDD2bYFjcWyrdImgcI9{ew>? NUXrR3N{OjR6NEKxOVg>
MDA-MB 231 NY[4PI14SXCxcITvd4l{KEG|c3H5 M1jpTVVuVQ>? M1jH[lAvPWh? M1HXZo1w\HWuYYTld{BVd2OxbXnuxs7DqGmwZIXj[YQh[XCxcITvd4l{ NFW1b3kzPDh|MEe4NS=>
Microglia  MlGwRZBweHSxc3nzJGF{e2G7 MUC1cW0> NFzt[oIzPGh? M2XnToRm[3KnYYPld{BpgXCxeHnhMYlv\HWlZXSgZ4VtdCCmZXH0bC=> NGrRcJUzPDhzOE[wNS=>
A2780cp M3LQdmFxd3C2b4Ppd{BCe3OjeR?= M{XPWlIvPW2P NXrpRodqOWh? Mkjo[IRJOk9? M1zsboVvcGGwY3XzJINqe3CuYYTpck1qdmS3Y3XkJINmdGxiZHXheIg> MkLlNlQ5OTd7NE[=
HepG2 NHPINnBHfW6ldHnvckBCe3OjeR?= NXmzXnV{PW2P M3O5XlRp M3rIbYlv[3KnYYPld{Bk\WyudXzhdkBt\X[nbIOgc4YhUE{EoB?= M1HFT|I1PzF|NUi3
U2OS MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4X3c|ExdU1? MkKwNlRp M2H5cIlvfGWwc3nmbYV{KHSqZTDndo94fGhiaX7obYJqfGmxbjDpcoR2[2WmIHL5JGRwgA>? MofwNlQ3OzlyMUO=
HCT116 Mkj1RZBweHSxc3nzJGF{e2G7 NYi3U4J1PW2P NI\y[48zPGh? Mn3SSG1UVw>? MlKx[Y5p[W6lZYOgZZBq\2WwaX6tbY5lfWOnZDDj[YxtKGSnYYTo NWX2Xm9lOjR4Mk[1NlI>
MCF-7  MkDIRZBweHSxc3nzJGF{e2G7 NIi0UpcxNjGvTR?= Ml3pOog> M1;QcYVvcGGwY3XzJJNqenSrbn;sMYlv\HWlZXSgZZBweHSxc3nz MVGyNlc2OTl6OR?=
PC-3  NEDqTHdCeG:ydH;zbZMhSXO|YYm= M3LkUlJuVQ>? NF\uTlgzcA>? MknnbY5kemWjc3XzJG9TUS2rbnT1Z4VlKGOnbHyg[IVifGh? NYLjPJFJOjJ5NEW1PFA>
U251  MnS3RZBweHSxc3nzJGF{e2G7 M4XmRlVuVQ>? NEfYdmkzPGh? NUDLNpBFcW6lcnXhd4V{KFNzLXnu[JVk\WRiY3XscEBl\WG2aB?= MYmyNlU4QTd6OB?=
HeLa  MXHBdI9xfG:|aYOgRZN{[Xl? NU\EWIMyPW2P MmXKNlRp NHTnSZZqdmS3Y3XzJINie3Cjc3Wt[IVx\W6mZX70JINmdGxiZHXheIg> M3yzUlIzPTR3MUK4
A549 MWLGeY5kfGmxbjDBd5NigQ>? Ml:1NE4ydU1? NEP0NVEzPGh? MoC1d5VxeHKnc4Pld{BUXTFzMke0MYlv\HWlZXSgZ4VtdCCmZXH0bC=> NEDNd44zOjR4Nkm2NC=>
pDCs NYHNV5p1TnWwY4Tpc44hSXO|YYm= NF7JU2MyOG2P NGfPOFkxNjWq M3HQdeKhemWmdXPld{B1cGViaX7keYN1cW:wIH;mJGlHVi4QsTDifUB{e1KQQUSw NIG5VlAzOjN7NkW5PS=>
BGC-823 Ml3mSpVv[3Srb36gRZN{[Xl? NI\kfW82dU1? NV3MdnhXOmh? M1PIZYlvcGmkaYTzJJRp\SC{YYTlJI9nKGG3dH;wbIFocWNiY3XscJM> NHO5cpozOjN{MkG1Ni=>
U937 MUfGeY5kfGmxbjDBd5NigQ>? MYOycW0> NILJ[XUyOmh? NUSwU|RT\GWlcnXhd4V{KHSqZTDheZRweGijZ4mgdoF1cW0EoB?= NUDsbHJSOjJzNUWxOVA>
Marc-145 NV\N[oVXTnWwY4Tpc44hSXO|YYm= MmizOY1O M1ry[lEzNzJ2L{O2bC=> Mn2ydoVlfWOnczD0bIUhWFKUU2[geIl1\XK|IHHu[EB1cGVicILveIVqdiCneIDy[ZN{cW:w NVf5OYJjOjJzMUm5NFA>
HBx  Mnz0RZBweHSxc3nzJGF{e2G7 MXSxNI1O NUTyb3REPDiq MorKSG1UVw>? M2LEPIlv[3KnYYPld{Bk\WyuIHTlZZRp Mo\UNlIxOjByN{i=
MCF-7 NEjOcVJHfW6ldHnvckBCe3OjeR?= NF7lZVIyOG2P MmW4OFhp NX3qb4V1[myxY3vzJIF2fG:yaHHnfUBqdmS3Y3XkJIJ6KGKxcoTlfo9ucWJ? M2KxTFIyQTNzOUO3
RMPI8226  NV2zT|ByTnWwY4Tpc44hSXO|YYm= MYO1cW0> MUixbC=> NInaR|R{fXCycnXzd4V{KHSqZTDs[ZZmdCCxZjDheZRweGijZ4mgeY5l\XJiboX0dolmdnRiZHXwcIV1cW:w M{nZW|IyQTF3NkKw
PC12/TetOn M3:4TGZ2dmO2aX;uJGF{e2G7 M3fTNlAvOS9zbV2= NYnWenVPOTiq Mn;vcIVi\HNidH:g{tEue3mwKGfULUBi[2O3bYXsZZRqd25uIITvfIlkcXS7LDDhcoQhd2yrZ3;t[ZIh\m:{bXH0bY9vyqB? MoTSNlE6ODZ4NUm=
HeLa  MYPDfZRwfG:6aXPpeJkhSXO|YYm= NX\KTZFqOm2P MortNlRp M1fFfolvcGmkaYTld{B1cGViY4n0c5RwgGmlaYT5JI9nKHOrbHnibY5qdiC2bzDI[WxiKGOnbHzzMi=> M2fXd|IyQDd3M{i1
Jurkat MoHqSpVv[3Srb36gRZN{[Xl? MVyxNI1O NGfGN28ycA>? MYHk[YNz\WG|ZYOgeIhmKGW6cILld5Nqd25ib3[gUGM{NUmLIHHu[EB1cGViZn;ycYF1cW:wIH;mJIF2fG:yaHHnc5NwdWW| NEKwdYMzOTh4NECzOy=>
K562 M1zHdWZ2dmO2aX;uJGF{e2G7 MVKxNI1O NWPRfno1OWh? MWfk[YNz\WG|ZYOgeIhmKGW6cILld5Nqd25ib3[gUGM{NUmLIHHu[EB1cGViZn;ycYF1cW:wIH;mJIF2fG:yaHHnc5NwdWW| MViyNVg3PDB|Nx?=
Nara-H NFvj[VZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX;vc2dMPW2P NX;j[pJ4PDiq NVvPT3Jz\W6qYX7j[ZMhfGWvc3nyc4xqdXW|bXXkbYF1\WRic4XwdJJme3Orb36gc4YhVmG{YT3IJINmdGxicILvcIln\XKjdHnvci=> NYLzVpVTOjF6MEWwN|M>
HUVECs NVfYeYZCTnWwY4Tpc44hSXO|YYm= MXWxNI1O MljnNE42cA>? NILaRmxl\WO{ZXHz[ZMhfGinIFHHSU1DW0GrbnT1Z4VlKGG3dH;wbIFogSCuZY\l NHTEdFYzOTR4OEW5Ni=>
HepG2 MYPBdI9xfG:|aYOgRZN{[Xl? MYmycW0> MVexbC=> Mono[Y5p[W6lZYOgdoFlcWG2aX;uMYlv\HWlZXSgZ4VtdCCmZXH0bC=> MWWyNVQ2OzZ7MR?=

... Click to View More Cell Line Experimental Data

In vivo 3-Methyladenine blocks autophagy through its effect on class III phosphatidylinositol 3-kinase (PI3K). 3-Methyladenine treatment does not alter the degree of hemorrhage compared with the subarachnoid hemorrhage (SAH) group. 3-Methyladenine pretreatment significantly aggravates neurological symptoms when compared with the SAH + vehicle group. Autophagy is decreased when 3-Methyladenine treatment is applied. Conversely, cleaved caspase-3 is markedly up-regulated in the SAH + 3-Methyladenine group. In line with the up-regulation of cleaved caspase-3 expression, the number of TUNEL-positive cells in the right cortex is significantly increased in the SAH + 3-Methyladenine group compared with the SAH + vehicle group. [5]

Protocol

Kinase Assay:[4]
+ Expand

Protein degradation assay:

HeLa cells are radiolabeled for 24 hours with 0.05 mCi/mL l-[U- 14C]valine. At the end of the labeling period, cells are rinsed three times with PBS. Cells are incubated for the designated times in either full medium or EBSS with or without the presence of 10 mM 3-Methyladenine.
Cell Research:[2]
+ Expand
  • Cell lines: HeLa cell line
  • Concentrations: 1-10 mM
  • Incubation Time: 24, 48 or 72 hours
  • Method: Cell (such as HeLa cell) viability is determined by a trypan blue exclusion assay. Briefly, after treated with 3-Methyladenine, both adherent and floating cells are collected and suspended in phosphate buffered saline (PBS, pH 7.4) at a final density of 1-2 × 106/mL. An equal volume of 0.4% trypan blue solution (w/v, in PBS) is added to the cell suspension and mixed thoroughly. After incubation at room temperature for 3 min, cell counting is performed using a hemacytometer.
    (Only for Reference)
Animal Research:[5]
+ Expand
  • Animal Models: Adult male Sprague–Dawley rats weighing 300-350 g
  • Formulation: Saline
  • Dosages: 400 nM
  • Administration: Intracerebral ventricular
    (Only for Reference)

Solubility (25°C)

In vitro Water 10 mg/mL warmed (67.04 mM)
Ethanol 4 mg/mL (26.81 mM)
DMSO 3 mg/mL warmed (20.11 mM)
In vivo Add solvents individually and in order:
water
10mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 149.15
Formula

C6H7N5

CAS No. 5142-23-4
Storage powder
Synonyms NSC 66389

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
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Molecular Weight Calculator

Molecular Weight Calculator

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Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    I'm also wondering whether it can be dissolved in water,or maybe something like culture medium,normal saline solution to form 10mM solution

  • Answer:

    As the reference (http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal. pone.0035665), 3-MA, which was found to inhibit autophagy at concentrations ranging from 1 to 10 mM was directly dissolved into the culture medium at the indicated concentrations. And we tested the solubility of S2767, and found the solubility of 3-MA in DMEM is 31 mg/mL at about 40°C.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID