3-Methyladenine (3-MA)

Catalog No.S2767 Synonyms: NSC 66389

3-Methyladenine (3-MA) Chemical Structure

Molecular Weight(MW): 149.15

3-Methyladenine (3-MA) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation.

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4 Customer Reviews

  • MDC-labeled vacuoles were induced by AZD2014 and inhibited by autophagy inhibitor (3-MA). SMMC-7721 cells were treated with AZD2014 or rapamycin at concentrations of 100 and 600 nM, respectively, for 48 hours in the presence or absence of 3-MA, and then stained with MDC. Cells were immediately observed under a confocal microscope. Cells in the control group were treated with DMSO. bars, 20 μm.

    Am J Cancer Res, 2015, 5(1): 125-139. 3-Methyladenine (3-MA) purchased from Selleck.

    Cells were treated with DMSO, TDCIPP (100 μM) only, or pretreated with 3-MA before treatment with TDCIPP (100 μM). LC3 conversion and beclin-1 and p62 expression were assessed by western blotting at 24 h

    Food Chem Toxicol, 2017, 100:183-196. 3-Methyladenine (3-MA) purchased from Selleck.

  • PC-9/ER cells were treated with CX-4945 (5 mM) for 48 h in the presence or absence of 3-MA (2 mM) and Atg7 siRNA (100 nM). Cleavage of PARP-1 and caspase-3 was shown by Western blot analysis. CT: without CX-4945; CX: CX-4945; CX+G: CX-4945 + gefitinib; CX+E: CX-4945 + erlotinib.

    PLoS One, 2014, 9(12): e114000 . 3-Methyladenine (3-MA) purchased from Selleck.

    A: The survival ratio of AML12 cells was measured after treatment with rapamycin or 3-MA; B: Western blot was used to detect the expression of LC3 and P62 in AML12 cells treated with Rapamycin or 3-MA.

    World J Gastroenterol, 2016, 22(18): 4501-4514.. 3-Methyladenine (3-MA) purchased from Selleck.

Purity & Quality Control

Choose Selective PI3K Inhibitors

Biological Activity

Description 3-Methyladenine (3-MA) is a selective PI3K inhibitor for Vps34 and PI3Kγ with IC50 of 25 μM and 60 μM in HeLa cells; blocks class I PI3K consistently, whereas suppression of class III PI3K is transient, and also blocks autophagosome formation.
Targets
Vps34 [1]
(HeLa cells)
PI3Kγ [1]
(HeLa cells)
25 μM 60 μM
In vitro

The slight preference for Vps34 prevention by 3-Methyladenine probably arises from a hydrophobic ring specific to Vps34, which encircles the 3-methyl group of 3-Methyladenine. [1] 3-Methyladenine has been reported to cause cancer cell death under both normal and starvation conditions. 3-Methyladenine could also suppress cell migration and invasion independently of its ability to inhibit autophagy, implying that 3-Methyladenine possesses functions other than autophagy suppression. 3-Methyladenine elicits caspase-dependent cell death that is independent of autophagy inhibition. Treatment with 5 mM 3-Methyladenine reduces the percentage of glucose-starved HeLa cells displaying GFP-LC3 puncta to 23%. The levels of LC3-I are increasing and the levels of LC3-II are decreasing between 12 and 48 hours in cells that are treated with 3-Methyladenine. Conversion of LC3-I to LC3-II is suppressed by 3-Methyladenine. Treatment of HeLa cells with 3-Methyladenine at 2.5 mM or 5 mM for one day does not affect cell viability, whereas treatment with 10 mM 3-Methyladenine for one day causes a 25.0% decrease in cell viability. Treatment of cells with 2.5, 5 or 10 mM 3-Methyladenine for two days causes 11.5%, 38.0% and 79.4% decrease in viability, respectively. 3-Methyladenine decreases cell viability in a time- and dose-dependent manner. 3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest. [2] Suppression of autophagy by 3-Methyladenine inhibits SU11274-induced cell death. [3] Prolonged treatment with 3-Methyladenine (up to 9 hours) induces significant LC3 I to II conversion in wild type MEFs. Prolonged treatment with 3-Methyladenine, but not wortmannin, markedly increases GFP-LC3 punctuation/aggregation. 3-Methyladenine-induced LC3 conversion and free GFP liberation are ATG7-dependent. 3-Methyladenine treatment leads to evident increase of p62 protein level. 3-Methyladenine increases the p62 level even in Atg5−/− MEFs as well as in cells with DOX-mediated deletion of ATG5. 3-Methyladenine inhibits class I and class III PI3K in different temporal patterns. 3-Methyladenine-induced LC3 I to LC3 II conversion is dramatically compromised in Tsc2−/− cells compared with wild type cells.3-Methyladenine disrupts the anti-autophagic function of mTOR complex 1. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
95D M4DPRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFi4[lU{dU1? Mn\KNog> NHP4V5NFVVOR M4C0TJJm\HWlZYOg[5Jwf3SqIHnubIljcXSxcomg[YZn\WO2IH;mJGJFVUN? MknxNlU4OTZ3NkG=
A549  MkezS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnHpN41O MmnwNog> M3fCNmROW09? MkGwdoVlfWOnczDndo94fGhiaX7obYJqfG:{eTDl[oZm[3Rib3[gRmROSw>? NYTJZlM3OjV5MU[1OlE>
95D M{LKO2Fxd3C2b4Ppd{BCe3OjeR?= MlW5OY1O MX2ybC=> Mn;ESG1UVw>? M4[1fIlvcGmkaYTzJGJFVUNvaX7keYNm\CCjcH;weI91cWNiY3XscEBl\WG2aB?= Mn[2NlU4OTZ3NkG=
A549  M3nJZmFxd3C2b4Ppd{BCe3OjeR?= MlzROY1O NIDsTHUzcA>? NIjvTGZFVVOR MkjQbY5pcWKrdIOgRmROSy2rbnT1Z4VlKGGyb4D0c5Rq[yClZXzsJIRm[XSq MmLqNlU4OTZ3NkG=
NBL-W-S  M3nuO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHjnWGgydU1? M{flW|Zp MXjlcohidmOnczDHRW5VNTZzIITvfIlkcXS7 NH;qcXIzPTN{M{KyNi=>
NBL-W-S  NFfabFFCeG:ydH;zbZMhSXO|YYm= M2jCTVFuVQ>? MWm2bC=> MV3pcoNz\WG|ZYOgZ4VtdCCjcH;weI9{cXNiaX7keYNm\CCkeTDHRW5VNTZz MlTyNlU{OjN{MkK=
A2780cp  MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIDqZmMydU1? MkLxNYg> NFu5SWNqdmO{ZXHz[ZMh[2m|cHzheIlvNWmwZIXj[YQh[2WubDDk[YF1cA>? MVKyOVMzOjZ7NB?=
A549  MnLDSpVv[3Srb36gRZN{[Xl? MkXROY1O NXLL[4E4Omh? NVvoU3hke3WycILld5NmeyCVUFOgbY5lfWOnZDDhZ4N2dXWuYYTpc44hd2ZiTFOzMWlK Mo\JNlUzQDV4Mki=
H157 NHjTZVdHfW6ldHnvckBCe3OjeR?= MXS1cW0> NUjFbIVPOmh? MX7zeZBxemW|c3XzJHNRSyCrbnT1Z4VlKGGlY4XteYxifGmxbjDv[kBNSzNvSVm= Mn3mNlUzQDV4Mki=
MDR MVzBdI9xfG:|aYOgRZN{[Xl? NEC1OYsyOG2P M1m5ZVZp NW\EW2JEe3S{ZX7neIhmdnNidHjlJJBwf2W{IH;mJIFvfGmlYX7j[ZIh\HK3Z4O= MkS1NlUxOTl5MEG=
HT-29  M3X0XWFxd3C2b4Ppd{BCe3OjeR?= MXmxcW0> NYT2N5c{PDiq MWLEUXNQ MmLD[Y5p[W6lZYOgZo9zfGW8b33pZk1qdmS3Y3XkJINmdGxidnnhZoltcXS7IHzvd5M> M3noSlI1QDR{MUW4
Microglia  MkKxRZBweHSxc3nzJGF{e2G7 MoC3OY1O M13CeVI1cA>? MYfk[YNz\WG|ZYOgbJlxd3irYT3pcoR2[2WmIHPlcIwh\GWjdHi= MVmyOFgyQDZyMR?=
A2780cp M2LZc2Fxd3C2b4Ppd{BCe3OjeR?= MoDpNk42dU1? Mor0NYg> NX2zPXhy\GSKMl:= M{LwbYVvcGGwY3XzJINqe3CuYYTpck1qdmS3Y3XkJINmdGxiZHXheIg> NVi0UGNOOjR6MUe5OFY>
HepG2 NF7pU|FHfW6ldHnvckBCe3OjeR?= NILLXZE2dU1? NG\lOIM1cA>? M{jJc4lv[3KnYYPld{BJVCC{ZXzlZZNm NHvSOFUzPDdzM{W4Oy=>
A549 MYLBdI9xfG:|aYOgRZN{[Xl? M3;6PFVuVQ>? NXzXbHlDPDiq NI\xNGxl\WO{ZXHz[ZMhfGinIIDldoNmdnSjZ3Wgc4Yh[2WubDDk[YF1cCCjbnSg[ZhxemW|c3nvckBt\X[nbIOgc4Yh[2G|cHHz[U0{NCCEZXPsbY4uOSCjbnSgUGM{NUmL MXqyOFcxPjNyMx?=
U2OS MoHERZBweHSxc3nzJGF{e2G7 NVridHpEOTCvTR?= M4LyZlI1cA>? NVyzNYdbcW62ZX7zbYZq\XNidHjlJIdzd3e2aDDpcohq[mm2aX;uJI9nKHSqZTDVNm9UKGOnbHzzJIlv\HWlZXSgZpkhTG:6 MXqyOFY{QTBzMx?=
Saos-2 M3v5WGFxd3C2b4Ppd{BCe3OjeR?= NYWxNnczOTCvTR?= NIDwUnQzPGh? NYLmfJg3cW62ZX7zbYZq\XNidHjlJIdzd3e2aDDpcohq[mm2aX;uJI9nKHSqZTDVNm9UKGOnbHzzJIlv\HWlZXSgZpkhTG:6 MXiyOFY{QTBzMx?=
A549 NYWyZnE{SXCxcITvd4l{KEG|c3H5 MnS3NVBuVQ>? M1HyflQ5cA>? MkTuZYNk\WyncnH0[ZMhfGinIILl[JVkfGmxbjDv[kBk\WyuII\pZYJqdGm2eTDpcoR2[2WmIHL5JHBVYA>? M2Xn[lI1PjJ4N{Ky
HCT116  MkT0RZBweHSxc3nzJGF{e2G7 NHO0elA2dU1? Mn\lNlRp MWDlcohidmOnczD0bIUh[XCxcITvd4l{KGmwZIXj[YQh[nliYYDp[4VvcW5? NFfjO3IzPDZ{NkWyNi=>
HGC-27 MUXGeY5kfGmxbjDBd5NigQ>? NXXtXHhOOTCvTR?= MWqxbC=> M16xbYlvcGmkaYTzJJRp\SClZXzsJJZq[WKrbHn0fUBtd3O|IHL5JHJCTDByMTDvdkBOUy1{MkC2 MmOyNlQ1OTZ|NEm=
U2OS  MXjGeY5kfGmxbjDBd5NigQ>? MXewMlUwOW2P M2rHW|Q5cA>? NE[2WnRqdmS3Y3XzJJNidGmwb335Z4lvNWmwZIXj[YQh[2WubDD2bYFjcWyrdImgcI9{ew>? MlTwNlQ{PTh|NEK=
MG-63 MXLGeY5kfGmxbjDBd5NigQ>? M{\Fd|AvPS9zbV2= M1;MV|Q5cA>? MmfPbY5lfWOnczDzZYxqdm:veXPpck1qdmS3Y3XkJINmdGxidnnhZoltcXS7IHzvd5M> NG[yToEzPDN3OEO0Ni=>
MG-63 M4XKbWFxd3C2b4Ppd{BCe3OjeR?= NYPzbZRTOC53L{HtUS=> NWSxeolUOzKq MnnF[Y5p[W6lZYOgd4FtcW6xbYnjbY4ucW6mdXPl[EBk\WyuIHHwc5B1d3Orcx?= NILsXW4zPDN3OEO0Ni=>
MG-63 MlPWRZBweHSxc3nzJGF{e2G7 MYGxNI1O M1TkWVEzcA>? NWPxPGlv\W6qYX7j[ZMhTFBvaX7keYNm\CCjcH;weI9{cXN? NWfUSHBsOjR|NUizNFE>
NTUB1 MVvBdI9xfG:|aYOgRZN{[Xl? M1X2TlVuVQ>? NWLiWpJTOS53aB?= NXn1OoM2eG:2ZX70bYF1\XNiY3Xs[YNwgGmkLXnu[JVk\WRiYYDvdJRwe2m| MmLQNlQ{PDlzN{[=
T24 NX3h[o9[SXCxcITvd4l{KEG|c3H5 MorpOY1O NXPtbo5DOS53aB?= NEG2c5Bxd3SnboTpZZRmeyClZXzlZ495cWJvaX7keYNm\CCjcH;weI9{cXN? MWqyOFM1QTF5Nh?=
SGC-7901  MoGwRZBweHSxc3nzJGF{e2G7 MoLENo1O NEXMbWQycA>? MlPZbY5kemWjc3XzJGNCNTRiaX7keYNm\CCjcH;weI9{cXN? MUiyOFMzOTN2MB?=
SMMC-7721 MXzBdI9xfG:|aYOgRZN{[Xl? Mn;oNo1O NVr2ZXdpOWh? MkjzbY5kemWjc3XzJGNCNTRiaX7keYNm\CCjcH;weI9{cXN? M1LtZVI1OzJzM{Sw
ECSCs  MoPjRZBweHSxc3nzJGF{e2G7 Ml\oNVBuVQ>? NEDse|A1cA>? NIfieodl\WO{ZXHz[ZMhemGyYX35Z4lvNXS{ZXH0[YQh[XCxcITvd4l{ MmXxNlQ{OTlzMEm=
MCF-7  Mlv4SpVv[3Srb36gRZN{[Xl? NWi3RW4{OTCvTR?= MoDnNlRp M3nuU4lvcGmkaYTzJJRp\SCjdYTvdIhi\3liaX7keYNm\CCkeTDjbIVud3SqZYLhdJkh\HK3Z4O= M3y1dlI1OzF3NUe4
UOK257 MkfiRZBweHSxc3nzJGF{e2G7 M{OySFVuVQ>? M2W5OlNp MkfC[Y5p[W6lZYOgdIFkdGm2YYjlcE1u\WSrYYTl[EBieG:ydH;zbZPDqA>? MY[yOFMxPTZyNB?=
ACHN-5968 M1jjOGFxd3C2b4Ppd{BCe3OjeR?= M4PUOVVuVQ>? NFHHUIU{cA>? NYWyXmE{\W6qYX7j[ZMheGGlbHn0ZZhmdC2vZXTpZZRm\CCjcH;weI9{cXQEoB?= MX6yOFMxPTZyNB?=
Huh7 NHrQbZlCeG:ydH;zbZMhSXO|YYm= MX2ycW0> NIjMV3cyOmh? NXzUdYY4TE2VTx?= M{LETYlvcGmkaYTzJGFbTDhyNUWtbY5lfWOnZDDj[YxtKGSnYYTo M4n6UVI1Ojl5M{Cw
Hep3B NInpfJlCeG:ydH;zbZMhSXO|YYm= M{PlNFJuVQ>? NYHpPIc1OTKq NV72NmVZTE2VTx?= NX3vXHJGcW6qaXLpeJMhSVqGOEC1OU1qdmS3Y3XkJINmdGxiZHXheIg> M37wTFI1Ojl5M{Cw
RKO M1jFRmZ2dmO2aX;uJGF{e2G7 NYrabXZuOm2P M2TZO|Fp M2rpZ2ROW09? MoTs[Y5p[W6lZYOgZ4VtdCCmZXH0bEBjgSCpZXzkZY5idXmlaX6= NYSybHZTOjR{OUG3O|c>
HepG2 E47 NWn4TFk6TnWwY4Tpc44hSXO|YYm= NUDsWpdPOi53bV2= MljZOFhp NYXNZlZtcW6lcnXhd4V{KHSqZTD0c5hq[2m2eTDv[kBCSSxiQmPPMEBidmRiQ1PsOC=> Mm[1NlQzPzN5M{i=
LoVo  NVPOR2VvSXCxcITvd4l{KEG|c3H5 NXLoO4x{PW2P MkDVOFhp Mknm[Y5p[W6lZYOgSGNCNWmwZIXj[YQh[XCxcITvd4l{Ng>? NV7hNY9XOjR{MEG4NVI>
WiDr NIezWXRHfW6ldHnvckBCe3OjeR?= NX;BUo9POTCvTR?= MYWxbC=> MWfpcohq[mm2czDQR2JNNWmwZIXj[YQhVEN|IFnJJIV5eHKnc4Ppc44> NYfxdXQ3OjRzOUC0PFk>
H1299  MXrGeY5kfGmxbjDBd5NigQ>? NVrIU|VCOTCvTR?= NIP1bpg1cA>? NFzt[XpqdmO{ZXHz[ZMh[2m|cHzheIlvNWmwZIXj[YQh[2WubDDk[YF1cA>? M1rLbVI1OTd|MkC4
H460 NYHYT3lITnWwY4Tpc44hSXO|YYm= MlnFNVBuVQ>? NIL3NZk1cA>? NWr0SYt2cW6lcnXhd4V{KGOrc4DsZZRqdi2rbnT1Z4VlKGOnbHyg[IVifGh? MkXRNlQyPzN{MEi=
A549 NYHKXXFjTnWwY4Tpc44hSXO|YYm= MYKxNI1O NFzJN4M1cA>? Mm\pbY5pcWKrdIOgZZV1d3CqYXf5JIlv\HWlZXSgZpkhcXK{YXTpZZRqd25? Mmf2NlQyPDJ5M{W=
Hep3B NUPsUXZYSXCxcITvd4l{KEG|c3H5 MlLvOY1O MWCyOIg> M{O3coF1fGWwdXH0[ZMhXE6ILd8xJJBzd3SnY4Tpc44h[WejaX7zeEB{\XK3bTDzeIFzfmG2aX;uMY1m\GmjdHXkJIFxd3C2b4Ppdy=> NUOy[XdEOjRyNk[2PVM>
SMMC-7721 M2rWNGFxd3C2b4Ppd{BCe3OjeR?= MkXGOY1O M2P2ZVI1cA>? Mn3hZZR1\W63YYTld{BVVkZvzsGgdJJwfGWldHnvckBi\2GrboP0JJNmenWvIIP0ZZJ3[XSrb36tcYVlcWG2ZXSgZZBweHSxc3nz Mn3ONlQxPjZ4OUO=
HO8910 MXTBdI9xfG:|aYOgRZN{[Xl? MYKxNI1O M1XQU|EzcA>? NEja[3hmdmijbnPld{BDOTlvaX7keYNm\CCjcH;weI9{cQ>? NFvZfJozOzl6M{[xNC=>
MCF7 NVLWbJBLTnWwY4Tpc44hSXO|YYm= M4W4cFVuVQ>? NGXjb2czPGh? NYDMc4hWcW6lcnXhd4V{KEO3TzDpcoR2[2WmIHPlcIwh\GWjdHi= MV[yN|k3OjZ{OR?=
HONE-1  MlzhSpVv[3Srb36gRZN{[Xl? NXvNelZVPW2P NELndWgycA>? M13JWZJmeHKnc4Pld{A3ei2vZXTpZZRm\CCUT2OgdJJw\HWldHnvci=> NUWzNnVIOjN6OUKzOVg>
HeLa NX7DVmxlTnWwY4Tpc44hSXO|YYm= NGDrNlIyOG2P NXW4PZIzOmh? NVrLfIs6e3WycILld5NmeyCOQ{OgTWkh\XiycnXzd4l{d25? MUGyN|g3PDd|OB?=
HepG2 NU\2R4dlTnWwY4Tpc44hSXO|YYm= MkXKNVBuVQ>? MmXYNlRp NHj2UVlqdmirYnn0d{B{cVSLR1HSMUBidmRiSFLTV{1qdmS3Y3XkJIF2fG:yaHHnfS=> MX[yN|gyPzB2MB?=
SH-SY5Y NWPmPVNpTnWwY4Tpc44hSXO|YYm= MYOxcW0> M1jSWlI1cA>? NFXWNmFqdmirYnn0d{B1cGViYYX0c5Bp[We7IHnu[JVk\WRiYomgWG9EWA>? NWDWXoFUOjN5NEOxOFg>
SH-SY5Y MYLBdI9xfG:|aYOgRZN{[Xl? NIfS[W82dU1? NH[4dWEycA>? NFXYXnJi[m:uaYPo[ZMh[2WuYYP0do9tKG6ndYLvdJJwfGWldHn2[UBm\m[nY4S= MXGyN|YyQTN7NR?=
PC12  NILQS2hHfW6ldHnvckBCe3OjeR?= M4P0WFExdU1? MWKyOIg> MmLDe4F1\XJ? M{TiZuKhcW6qaXLpeJMh[2i7bX;0dplxe2mwLXzpb4UheHKxdHXhd49u[WxiYXP0bZZqfHlw NHnMN2QzOzZyM{m3PS=>
OV2008 NWnzZZZoSXCxcITvd4l{KEG|c3H5 NFTwWYs2dU1? MX2yOIg> M1;Yd4Nwdn[ncoTzJGZVYTd{MDD3bZRpKEOGRGCgbY51dyCjbjDh[IRqfGm4ZTDl[oZm[3RidH;3ZZJleyCtaXzsbY5oKG:4YYLpZY4h[2GwY3XyJINmdGy| NVy1PHB7OjN3OUKyPFE>
A2780 M3PqdmFxd3C2b4Ppd{BCe3OjeR?= M1rkZVVuVQ>? MY[yOIg> NXf1XZBP[2:wdnXyeJMhTlS\N{KwJJdqfGhiQ1TEVEBqdnSxIHHuJIFl\Gm2aY\lJIVn\mWldDD0c5diemS|IHvpcIxqdmdib4\hdolidiClYX7j[ZIh[2WubIO= MkLxNlM2QTJ{OEG=
Saos-2  M2nn[2Fxd3C2b4Ppd{BCe3OjeR?= MkLINY1O MYO5Oog> NEfQbo9qdmO{ZXHz[ZMh[2WubDDk[YF1cCCrbnT1Z4VlKGK7IGDDXC=> MX:yN|U3OzF5MR?=
1321N1 NUjBNFVuS3m2b4TvfIlkcXS7IFHzd4F6 NYnI[G1MPW2P NWTpUFZZOjSq MVTwdo91\WO2czDj[YxtKGGpYXnud5QhWEOQLXnu[JVk\WRidH;4bYNqfHl? NWLPTmdxOjN3MkWyOlU>
SH-SY5Y Mn:1R5l1d3SxeHnjbZR6KEG|c3H5 MVG1cW0> NIT5N2UzPGh? NGGy[ndqdmO{ZXHz[ZMhWEOQIITvfIlkcXS7 M33mWFI{PTJ3Mk[1
HT-29  NIXxZ2tHfW6ldHnvckBCe3OjeR?= MnWwNY1O M{DpeVQ5Nzl4aB?= MXPpcohq[mm2czDBUXBMKGmwZIXj[ZMh[XW2b4DoZYdq[yClZXzsJIRm[XSq NWf0PXhQOjN3MEiyO|I>
OR6 NIHs[FhHfW6ldHnvckBCe3OjeR?= MU[xNI1O MoG3O|Jp NX34S5BWe3WycILld5NmeyCKQ2[gdoVxdGmlYYTpc44h[W6mIH\vdo1ifGmxbjDv[kBifXSxcHjh[49{d22ncx?= MmHNNlM{QTV6N{W=
Hela  NXTNOGFwTnWwY4Tpc44hSXO|YYm= NF\KTWw2dU1? NVXaT496OjSq NVjFOnkzcW6qaXLpeJMhe3Sjco\heIlwdi2rbnT1Z4VlKGG3dH;wbIFogQ>? MXmyN|M6PTZ5OR?=
MCF-7  Mn3DSpVv[3Srb36gRZN{[Xl? MWK1cW0> M17UVVI1cA>? NYTueog4cW6qaXLpeJMhe3Sjco\heIlwdi2rbnT1Z4VlKGG3dH;wbIFogQ>? MUGyN|M6PTZ5OR?=
HUVECs MoLLSpVv[3Srb36gRZN{[Xl? Mke5N41O MlTmNlRp MXjicI9kc3NidHjlJJBzd3SnY4TpeoUh\W[oZXP0JI9nKHKnc4\ldoF1em:uIHL5JIlvcGmkaYTpcoch[XW2b4DoZYd6 Mm\DNlM{PTh7Mki=
T24 NFzqTmRHfW6ldHnvckBCe3OjeR?= MYKxNI1O MX:xbC=> MWry[YR2[2W|IITo[UBkdGWjdnHn[UBw\iCOQ{OgZYZ1\XJiYnHpZ4FtcW5idILlZZRu\W62 MnziNlM{PTRyOEC=
U251MG  M4jPNGZ2dmO2aX;uJGF{e2G7 NEPreIw{dU1? M33wXlFp M3n1bZN2eHC{ZYPz[ZMhVEN|LVnJJJBzd3SnaX6g[ZhxemW|c3nvci=> NWP3UHM6OjN|M{i2NVg>
GTL-16  MUXBdI9xfG:|aYOgRZN{[Xl? NUj6O4pxPW2P NXr2eY5pOjSq MVLy[YR2[2W|IHPlcIwhfmmjYnnsbZR6KGG|IHPvcZBiemWmIITvJINmdGy|IITy[YF1\WRid3n0bEBOTVRiaX7obYJqfG:{cx?= M2jsR|I{OzF|NEmw
T-47D NYrJSGl{TnWwY4Tpc44hSXO|YYm= M13NWFExdU1? MlzqNog> M3vhe4lvcGmkaYTzJIF2fG:yaHHnfUBxem:lZYPzJIFv\CCrbnPy[YF{\XNicnHwZY16[2mwIHnu[JVk\WRiYYDvdJRwe2m| Mlq1NlM{ODByMk[=
PaCa44 NV3CXWw3SXCxcITvd4l{KEG|c3H5 MXeyMlVuVQ>? M2Hx[FFp NWO3[odIemWmdXPld:Kh\2WwaYDpck1u\WSrYYTl[EBieG:ydH;zbZM> MWqyN|EzPDFzMh?=
MDA-MB231 MVPGeY5kfGmxbjDBd5NigQ>? NWPTVWFuPW2P NYPLVWQ{OWh? MYTpcoNz\WG|ZYOgdoV{fmW{YYTyc4wudWWmaXH0[YQh[2G|cHHz[UBi[3SrdnH0bY9vKGGwZDDj[YxtKGSnYYTo NVjuVWg4OjNyOEi4OVA>
HeLa M1P3N2Fxd3C2b4Ppd{BCe3OjeR?= NFLtVWYyOG2P M1HtOVJp NHLZXXFl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgZ48ufHKnYYTt[Y51KHerdHigVGVK NV;ie4pmOjNyMECxN|U>
SK-HEP-1 NH;IcJlCeG:ydH;zbZMhSXO|YYm= NFfWWYYyOG2P MnLhNYg> MmfadJJwfGWldIOgZYdicW6|dDDheZRweGijZ4mgZY5lKGmwZIXj[ZMh[XCxcITvd4l{KGmwIHL1[oFtcW5vdILlZZRm\CClZXzsdy=> M1;FV|IzQDV6NkS5
HepG2 M4Ho[mFxd3C2b4Ppd{BCe3OjeR?= NHrsVFE{dU1? M{HsWlVp NViyTVg5emWmdXPld{Bk\WyuIHHwc5B1d3OrczDpcoR2[2WmIHL5JHFFew>? MV6yNlg{PjV7NR?=
MCF-7  NUSyeFV5TnWwY4Tpc44hSXO|YYm= MVqycW0> MoLuNlRp NWHme2JwcW6qaXLpeJMh[XW2b4DoZYd6KGmwZIXj[YQh[nliVF5CpC=> MVqyOFk4ODZ5Nh?=
MDA-MB-231 NH7VR|FHfW6ldHnvckBCe3OjeR?= NITmZ2szdU1? MVqyOIg> MlnNbY5pcWKrdIOgZZV1d3CqYXf5JIlv\HWlZXSgZpkhXE4EoB?= MnPaNlQ6PzB4N{[=
MCF-7  M4fJbmZ2dmO2aX;uJGF{e2G7 NYC3UFNCOm2P M3T6fVQ5cA>? NVG3fG9OeHKxbX;0[ZMhXE1vaX7keYNm\CClZXzsJIRm[XSq NISzT5AzPDl5ME[3Oi=>
MDA-MB-231 MmPJSpVv[3Srb36gRZN{[Xl? NELuNWIzdU1? MoDxOFhp NWi1SWs5eHKxbX;0[ZMhXE1vaX7keYNm\CClZXzsJIRm[XSq NGK5eI0zPDl5ME[3Oi=>
PANC-1  NUXzRVJzSXCxcITvd4l{KEG|c3H5 NWLRbpMxOW2P NVSzO3BPPDiq NISzXHlFVVOR NETjcGNmdmijbnPld{Bjd3K2ZYrvcYljNWmwZIXj[YQh[2WubDD2bYFjcWyrdImgcI9{ew>? MXyyOFg1OjF3OB?=
MDA-MB 231 MlvlRZBweHSxc3nzJGF{e2G7 MX61cW0> MoPENE42cA>? MUTtc4R2dGG2ZYOgWI9kd22rbtMuxsBqdmS3Y3XkJIFxd3C2b4Ppdy=> M3TDRVI1QDNyN{ix
Microglia  MoTnRZBweHSxc3nzJGF{e2G7 M{nOTFVuVQ>? NF[5ZWEzPGh? MXHk[YNz\WG|ZYOgbJlxd3irYT3pcoR2[2WmIHPlcIwh\GWjdHi= M1PVb|I1QDF6NkCx
A2780cp M3fvUWFxd3C2b4Ppd{BCe3OjeR?= MVWyMlVuVQ>? MlPQNYg> M3\TW4RlUDKR MYnlcohidmOnczDjbZNxdGG2aX6tbY5lfWOnZDDj[YxtKGSnYYTo MXeyOFgyPzl2Nh?=
HepG2 NFH6O5lHfW6ldHnvckBCe3OjeR?= M2izWVVuVQ>? MYq0bC=> NGD3PVNqdmO{ZXHz[ZMh[2WubIXsZZIhdGW4ZXzzJI9nKEiOwrC= MUSyOFcyOzV6Nx?=
U2OS M1rlNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mmq4NVBuVQ>? M3[1OlI1cA>? NFHLOppqdnSnboPp[olmeyC2aHWg[5Jwf3SqIHnubIljcXSrb36gbY5lfWOnZDDifUBFd3h? M4X6XVI1PjN7MEGz
HCT116 NWPtSZVSSXCxcITvd4l{KEG|c3H5 M3zaZlVuVQ>? MoWzNlRp MojESG1UVw>? MVvlcohidmOnczDhdIlo\W6rbj3pcoR2[2WmIHPlcIwh\GWjdHi= MYmyOFYzPjV{Mh?=
MCF-7  MYTBdI9xfG:|aYOgRZN{[Xl? MVywMlFuVQ>? NYe1cXVLPmh? Ml3j[Y5p[W6lZYOgd4lzfGmwb3ytbY5lfWOnZDDhdI9xfG:|aYO= NXHRWI9MOjJ5NUG5PFk>
PC-3  NUH4VHc2SXCxcITvd4l{KEG|c3H5 NFHLRlAzdU1? MoLXNog> M4HjdIlv[3KnYYPld{BQWklvaX7keYNm\CClZXzsJIRm[XSq NF3UVpYzOjd2NUW4NC=>
U251  M3n6VWFxd3C2b4Ppd{BCe3OjeR?= NUTWT4FYPW2P MYKyOIg> NXLDPWNvcW6lcnXhd4V{KFNzLXnu[JVk\WRiY3XscEBl\WG2aB?= NF;nbXkzOjV5OUe4PC=>
HeLa  NUXSNpVYSXCxcITvd4l{KEG|c3H5 NWWwZoVwPW2P MnH5NlRp NHG5eXdqdmS3Y3XzJINie3Cjc3Wt[IVx\W6mZX70JINmdGxiZHXheIg> NHrDVokzOjV2NUGyPC=>
A549 MnX3SpVv[3Srb36gRZN{[Xl? NUnRfogxOC5zbV2= M{DaNlI1cA>? M17xfJN2eHC{ZYPz[ZMhW1VzMUK3OE1qdmS3Y3XkJINmdGxiZHXheIg> MmKwNlI1PjZ7NkC=
pDCs NHfRVnZHfW6ldHnvckBCe3OjeR?= M3;qb|ExdU1? MojTNE42cA>? Mn3VxsBz\WS3Y3XzJJRp\SCrbnT1Z5Rqd25ib3[gTWZPNc7zIHL5JJN{Wk6DNEC= NXXnXJV4OjJ|OU[1PVk>
BGC-823 NWLlbJk{TnWwY4Tpc44hSXO|YYm= NHzLRYg2dU1? NVfLZnM1Omh? NWDaUm54cW6qaXLpeJMhfGinIILheIUhd2ZiYYX0c5Bp[WerYzDj[Yxtew>? M2PQNFIzOzJ{MUWy
U937 M3rHZmZ2dmO2aX;uJGF{e2G7 MoX1No1O MYCxNog> M3XXOYRm[3KnYYPld{B1cGViYYX0c5Bp[We7IILheIlwyqB? NXnle4VlOjJzNUWxOVA>
Marc-145 NFvCc5hHfW6ldHnvckBCe3OjeR?= NHvsXlY2dU1? MnHQNVIwOjRxM{\o NF3rTndz\WS3Y3XzJJRp\SCSUmLTWkB1cXSncoOgZY5lKHSqZTDwdo91\WmwIHX4dJJme3Orb36= NVfzb|FyOjJzMUm5NFA>
HBx  NGewZY1CeG:ydH;zbZMhSXO|YYm= M1HzO|ExdU1? M2rvUFQ5cA>? MkjvSG1UVw>? MXPpcoNz\WG|ZYOgZ4VtdCCmZXH0bC=> MonmNlIxOjByN{i=
MCF-7 NF3TW5dHfW6ldHnvckBCe3OjeR?= NWHWdnBkOTCvTR?= NUO2c2prPDiq MWTicI9kc3NiYYX0c5Bp[We7IHnu[JVk\WRiYomgZo9zfGW8b33pZi=> MV:yNVk{OTl|Nx?=
RMPI8226  NHzuc2dHfW6ldHnvckBCe3OjeR?= M{fC[VVuVQ>? NXLSTYtpOWh? MmPpd5VxeHKnc4Pld{B1cGVibHX2[Ywhd2ZiYYX0c5Bp[We7IIXu[IVzKG63dILp[Y51KGSncHzleIlwdg>? M{nHdFIyQTF3NkKw
PC12/TetOn NWq1XYtrTnWwY4Tpc44hSXO|YYm= Mnz6NE4yNzGvTR?= MUCxPIg> MoDicIVi\HNidH:g{tEue3mwKGfULUBi[2O3bYXsZZRqd25uIITvfIlkcXS7LDDhcoQhd2yrZ3;t[ZIh\m:{bXH0bY9vyqB? NGLM[HEzOTlyNk[1PS=>
HeLa  NGrUZYlEgXSxdH;4bYNqfHliQYPzZZk> MnjuNo1O Mlq4NlRp MWXpcohq[mm2ZYOgeIhmKGO7dH;0c5hq[2m2eTDv[kB{cWyrYnnubY4hfG9iSHXMZUBk\Wyucz6= NXq0[HFxOjF6N{WzPFU>
Jurkat MoK0SpVv[3Srb36gRZN{[Xl? M4rVOVExdU1? NH\qUIsycA>? NHrpNVhl\WO{ZXHz[ZMhfGinIHX4dJJme3Orb36gc4YhVEN|LVnJJIFv\CC2aHWg[o9zdWG2aX;uJI9nKGG3dH;wbIFod3OxbXXz MmnCNlE5PjRyM{e=
K562 NYrzdJBNTnWwY4Tpc44hSXO|YYm= NUDrW3Q4OTCvTR?= MYexbC=> M2OwNoRm[3KnYYPld{B1cGViZYjwdoV{e2mxbjDv[kBNSzNvSVmgZY5lKHSqZTDmc5Ju[XSrb36gc4Yh[XW2b4DoZYdwe2:vZYO= MVmyNVg3PDB|Nx?=
Nara-H Mk[0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2G2V|VuVQ>? Mn;sOFhp NYnR[FJ6\W6qYX7j[ZMhfGWvc3nyc4xqdXW|bXXkbYF1\WRic4XwdJJme3Orb36gc4YhVmG{YT3IJINmdGxicILvcIln\XKjdHnvci=> MnvrNlE5ODVyM{O=
HUVECs M3;1cWZ2dmO2aX;uJGF{e2G7 M4W4TlExdU1? NWnsclVjOC53aB?= NWnEbYFt\GWlcnXhd4V{KHSqZTDBS2UuSlODaX7keYNm\CCjdYTvdIhi\3libHX2[S=> NIL5VY4zOTR4OEW5Ni=>
HepG2 M13MT2Fxd3C2b4Ppd{BCe3OjeR?= MVSycW0> Mn3JNYg> NEfDcIdmdmijbnPld{Bz[WSrYYTpc44ucW6mdXPl[EBk\WyuIHTlZZRp NVHHNIlmOjF2NUO2PVE>

... Click to View More Cell Line Experimental Data

In vivo 3-Methyladenine blocks autophagy through its effect on class III phosphatidylinositol 3-kinase (PI3K). 3-Methyladenine treatment does not alter the degree of hemorrhage compared with the subarachnoid hemorrhage (SAH) group. 3-Methyladenine pretreatment significantly aggravates neurological symptoms when compared with the SAH + vehicle group. Autophagy is decreased when 3-Methyladenine treatment is applied. Conversely, cleaved caspase-3 is markedly up-regulated in the SAH + 3-Methyladenine group. In line with the up-regulation of cleaved caspase-3 expression, the number of TUNEL-positive cells in the right cortex is significantly increased in the SAH + 3-Methyladenine group compared with the SAH + vehicle group. [5]

Protocol

Kinase Assay:[4]
+ Expand

Protein degradation assay:

HeLa cells are radiolabeled for 24 hours with 0.05 mCi/mL l-[U- 14C]valine. At the end of the labeling period, cells are rinsed three times with PBS. Cells are incubated for the designated times in either full medium or EBSS with or without the presence of 10 mM 3-Methyladenine.
Cell Research:[2]
+ Expand
  • Cell lines: HeLa cell line
  • Concentrations: 1-10 mM
  • Incubation Time: 24, 48 or 72 hours
  • Method: Cell (such as HeLa cell) viability is determined by a trypan blue exclusion assay. Briefly, after treated with 3-Methyladenine, both adherent and floating cells are collected and suspended in phosphate buffered saline (PBS, pH 7.4) at a final density of 1-2 × 106/mL. An equal volume of 0.4% trypan blue solution (w/v, in PBS) is added to the cell suspension and mixed thoroughly. After incubation at room temperature for 3 min, cell counting is performed using a hemacytometer.
    (Only for Reference)
Animal Research:[5]
+ Expand
  • Animal Models: Adult male Sprague–Dawley rats weighing 300-350 g
  • Formulation: Saline
  • Dosages: 400 nM
  • Administration: Intracerebral ventricular
    (Only for Reference)

Solubility (25°C)

In vitro Water 10 mg/mL warmed (67.04 mM)
Ethanol 4 mg/mL (26.81 mM)
DMSO 3 mg/mL warmed (20.11 mM)
In vivo Add solvents individually and in order:
water
10mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 149.15
Formula

C6H7N5

CAS No. 5142-23-4
Storage powder
Synonyms NSC 66389

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
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Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    I'm also wondering whether it can be dissolved in water,or maybe something like culture medium,normal saline solution to form 10mM solution

  • Answer:

    As the reference (http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal. pone.0035665), 3-MA, which was found to inhibit autophagy at concentrations ranging from 1 to 10 mM was directly dissolved into the culture medium at the indicated concentrations. And we tested the solubility of S2767, and found the solubility of 3-MA in DMEM is 31 mg/mL at about 40°C.

PI3K Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID