Catalog No.S7312 Synonyms: Caspase-3 Inhibitor
Molecular Weight(MW): 668.66
Z-DEVD-FMK is a specific, irreversible Caspase-3 inhibitor, and also shows potent inhibition on caspase-6, caspase-7, caspase-8, and caspase-10.
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Immunoblotting analysis was performed for the active-caspase-3 and cleavage of MCL-1 in MM.1S cells treated with z-DEVD-fmk (20 μM, 1 h) and following GSK126 (25 μM, 24 h).
Oncotarget, 2017, 8(2):3396-3411. Z-DEVD-FMK purchased from Selleck.
(J,K)After pre-treated with Caspase-3 inhibitor Z-DEVD-FMK 50μM, the apoptotic rates of cells induced by E Platinum in BGC-823, MGC-803, and SGC-7901 cells were detected by Annexin V/PI double-staining assay. Ann.V-/PI- as healthy cells, Ann.+/PI- as early apoptotic cells, Ann.+/PI+ probably as late apoptotic cells. Data were shown as means SD for three independent experiments (*P<0.05 and **P<0.01 compared with control, ##P<0.01 compared to Z-DEVD-FMK+40 μM E Platinum group with treatment of 40 μM E Platinum).
Mol Carcinog, 2016, 56(1):218-231. Z-DEVD-FMK purchased from Selleck.
Caspase activation degrades Beclin-1. (A and B) After pretreatment with z-VAD-fmk (20 μM) or z-DEVD-fmk (20 μM) for 1 h, K562 cells were exposed to BIIB021 (400 nM) while 32Dp210-T315I cells were exposed to BIIB021 (200 nM) for 24 h. Whole-cell lysates were subjected to western blot analysis to examine the expression of Beclin-1, LC3I/II, p62, PARP and caspase-3.
INTERNATIONAL JOURNAL OF ONCOLOGY, 2016, 48:1710-1720.. Z-DEVD-FMK purchased from Selleck.
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Choose Selective Caspase Inhibitors
|Description||Z-DEVD-FMK is a specific, irreversible Caspase-3 inhibitor, and also shows potent inhibition on caspase-6, caspase-7, caspase-8, and caspase-10.|
Z-DEVD-FMK (1–200 μM) inhibits D4-GDI cleavage and apoptosis in a concentration-dependent manner.  Z-DEVD-FMK reduces ceramide-induced cardiomyocyte death and significantly inhibits the activation of caspase 3.  Z-DEVD-FMK (100μM) attenuates OxyHb-induced cell detachment, reduced caspase-2 and -3 activities, abolishes OxyHb-induced DNA ladders, and prevents OxyHb-induced cleavage of PARP in cultured brain microvessel endothelial cells.  Z-DEVD-FMK (100 μM) blocks MPP+-induced increases in caspase-3 enzyme activity. Z-DEVD-FMK dose dependently blocks 6-OHDA-induced apoptotic cell death with IC50 of 18 μM. 
|In vivo||Z-DEVD-FMK, before and after injury, markedly reduces post-traumatic apoptosis, and significantly improved neurological recovery. |
Caspase activity assay :Caspase-3 and caspase-9 activities are measured using fluorescent-based substrate. After treatment, the cells are resuspended in lysis buffer (50 mM Tris HCl, 1 mM EDTA, and 10 mM EGTA) containing 10 mM digitonin for 20 min at 37°C. Supernatants are treated with either of the fluorogenic substrates Ac-DEVD-AFC for caspase-3 or Ac-LEHD-AFC for caspase-9 for 1 h at 37°C and fluorescence is measured at excitation at 400 nm and emission at 505 nm using a Gemini XS fluorescence plate reade
-  Rickers A, et al. Eur J Immunol. 1998, (1), 296-304.
-  Yakovlev AG, et al. J Neurosci. 1997, 17(19), 7415-7424.
-  Wang J, et al. J Card Fail. 2000, 6(3), 243-249.
|In vitro||DMSO||100 mg/mL (149.55 mM)|
|In vivo||Add solvents to the product individually and in order:
2% DMSO+30% PEG 400+5% Tween 80+ddH2O
For best results, use promptly after mixing.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).
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