SH-4-54

Catalog No.S7337

SH-4-54 Chemical Structure

Molecular Weight(MW): 610.59

4 Customer Reviews

  • Expression of indicated proteins in PRL-stimulated INS-1 cells without (control, white bars) or with (black bars) STAT inhibitor (SH-4-54).

    Diabetologia, 2015, 58: 2064–2073. SH-4-54 purchased from Selleck.

    After cells exposed to 20 μM SH-4-54 for 24 hr, (a) Western blot analysis was conducted to determine protein expression of p-Stat5 at Tyr694, Stat5, Akt1, Akt2, and Akt, and (b) RT-PCR was performed to determine mRNA expression of Akt1/2. After cells transfected with si-Stat5/si-NC.

    Sci Rep, 2016, 6:33358.. SH-4-54 purchased from Selleck.

  • Pancreatic acini exposed for 1 h to 300 μM of oleic acid (OA) or linoleic acid (LA) were pre-treated for 45 min with the vehicle (DMSO), JSH-23 (30 μM) or SH-4-54 (10 μM). CCL2 mRNA expression was analyzed by qRT-PCR with 18S as internal standard. *p<0.05, **p<0.01 as compared with untreated acini, ♦♦p<0.01 as compared with OA- or LA-treated acini.

    Biochim Biophys Acta, 2015, 1852(12):2671-7. SH-4-54 purchased from Selleck.

    Influence of small molecule inhibitors on IFN-g production in MAIT cells. (A to F) Representative flow plots showing IFN-g production in MAIT cells stimulated with Mtb lysates/IL-15, in the presence of DMSO alone (A), JNK1/2/3 inhibitor SP600125 (B), NFkB inhibitor BAY11-7802 (C), p38 MAPK inhibitor SB203580 (D), PI3 Kinase p100a/d/b inhibitor LY294002 (E), or STAT3/STAT5 inhibitor SH-4-54 (F). (G) Comparison of inhibition effect on IFN-g production in MAIT cells in the presence of DMSO (solvent for small molecule inhibitors), or different small molecule inhibitors (nZ6). In the figure, error bars indicate SEM. Paired t-test was used for statistical analysis between groups.

    J Infect, 2016, 72(3):338-52.. SH-4-54 purchased from Selleck.

Purity & Quality Control

Choose Selective STAT Inhibitors

Biological Activity

Description SH-4-54 is a potent STAT inhibitor with KD of 300 nM and 464 nM for STAT3 and STAT5, respectively.
Targets
STAT3 [1] STAT5 [1]
300 nM(Kd) 464 nM(Kd)
In vitro

SH-4-54 shows unprecedented cytotoxicity in human glioblastoma brain cancer stem cells (BTSCs), while has no toxicity in human fetal astrocytes. In addition, SH-4-54 effectively suppresses STAT3 phosphorylation and its downstream transcriptional targets. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human 127EF cells NGPYVlVEgXSxdH;4bYPDqGG|c3H5 NETtWmQ{KGSjeYO= M2XuSmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJFEzP0WIIHPlcIx{KGG|c3Xzd4VlKGG|IHPlcIwhfmmjYnnsbZR6KGGodHXyJFMh\GG7czDifUBCdGGvYYKgRox2\SCjc4PhfUwhUUN3ME22OkBvVQ>? M{nnZlI1QTByNkGy
human 30M cells NVvJUW1iS3m2b4TvfIlkyqCjc4PhfS=> MWSzJIRigXN? Mn;oR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gN|BOKGOnbHzzJIF{e2W|c3XkJIF{KGOnbHygeoli[mmuaYT5JIFnfGW{IEOg[IF6eyCkeTDBcIFu[XJiQnz1[UBie3OjeTygSWM2OD1yLkGg{txO MV2yOFkxODZzMh?=
human 84EF cells M1f1eGN6fG:2b4jpZ:Kh[XO|YYm= M4O1dVMh\GG7cx?= MkXpR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gPFRGTiClZXzsd{Bie3Onc4Pl[EBieyClZXzsJJZq[WKrbHn0fUBi\nSncjCzJIRigXNiYomgRYxidWG{IFLseYUh[XO|YYmsJGlEPTB;MD6xNFIh|ryP NXfwXVBZOjR7MEC2NVI>
human 67EF cells NVjaU5N3S3m2b4TvfIlkyqCjc4PhfS=> MmTuN{Bl[Xm| MVrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjC2O2VHKGOnbHzzJIF{e2W|c3XkJIF{KGOnbHygeoli[mmuaYT5JIFnfGW{IEOg[IF6eyCkeTDBcIFu[XJiQnz1[UBie3OjeTygTWM2OD1yLkGwOkDPxE1? NX\CTHdLOjR7MEC2NVI>
human 73EF cells MWXDfZRwfG:6aXRCpIF{e2G7 MkjuN{Bl[Xm| NVX5d2doS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hPzOHRjDj[YxteyCjc4Pld5Nm\CCjczDj[YxtKH[rYXLpcIl1gSCjZoTldkA{KGSjeYOgZpkhSWyjbXHyJGJtfWViYYPzZZktKEmFNUC9NE4yPjJizszN MV2yOFkxODZzMh?=
human 25EF cells MXnDfZRwfG:6aXRCpIF{e2G7 MnrkN{Bl[Xm| NHjkWlBEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckAzPUWIIHPlcIx{KGG|c3Xzd4VlKGG|IHPlcIwhfmmjYnnsbZR6KGGodHXyJFMh\GG7czDifUBCdGGvYYKgRox2\SCjc4PhfUwhUUN3ME2wMlI{PCEQvF2= MlPONlQ6ODB4MUK=
human 73M cells MUDDfZRwfG:6aXRCpIF{e2G7 M{LERlMh\GG7cx?= NWnuc2RtS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hPzOPIHPlcIx{KGG|c3Xzd4VlKGG|IHPlcIwhfmmjYnnsbZR6KGGodHXyJFgh\GG7czDifUBCdGGvYYKgRox2\SCjc4PhfUwhUUN3ME2xMlA{KM7:TR?= NV2ybYVROjR7MEC2NVI>
human 147EF cells M4joU2Z2dmO2aX;uJIF{e2G7 NVvLU2I4Oi15MjDo MXzJcohq[mm2aX;uJI9nKFOWQWSzJIlvKGi3bXHuJFE1P0WIIHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDv[kBxcG:|cHjvdplt[XSnZDDjfYNtcW5iREGgcIV3\WxiYX\0[ZIhOiC2bzC3NkBpenNiYomgW4V{fGW{bjDicI91fGmwZzDhcoFtgXOrcx?= MVWyOFkxODZzMh?=

... Click to View More Cell Line Experimental Data

In vivo In mice orthotopically xenografted with BT73, SH-4-54 (10 mg/kg i.p.) exhibits BBB permeability, potently suppresses glioma tumor growth, and inhibits pSTAT3. [1]

Protocol

Kinase Assay:[1]
+ Expand

Surface Plasmon Resonance (SPR) studies:

The binding experiments are carried out on a ProteOn XPR36 biosensor at 25°C using the HTE sensor chip. The flow cells of the sensor chip are loaded with a nickel solution at 30 μL/min for 120 s to saturate the Tris–NTA surface with Ni(II) ions. Purified His-tagged STAT3 and STAT5 in PBST buffer (PBS with 0.005% (v/v) Tween-20 and 0.001% DMSO pH 7.4) is injected in the first and second channels of the chip respectively in the vertical direction at a flow rate of 25 μg/μL for 300 s, which attained, on average, ~8000 resonance unit (RU). After a wash with PBST buffer, inhibitors binding to the immobilized proteins is monitored by injecting a range of concentrations along with a blank at a flow rate of 100 μL/min for 200 s for each of these small molecules. When the injection of the small molecule inhibitor is completed, running buffer is allowed to flow over the immobilized substrates for the non-specifically bound inhibitors to dissociate for 600 s. Following dissociation of the inhibitors, the chip surface is regenerated with an injection of 1 M NaCl at a flow rate of 100 μL/ml for 18 s. Interspot channel reference is used for non-specific binding corrections and the blank channel used with each analyte injection served as a double reference to correct for possible baseline drift. Data are analyzed using ProteOn Manager Software version 3.1. The Langmuir 1:1 binding model was used to determine the KD values.
Cell Research:sup>[1]
+ Expand
  • Cell lines: BTSC lines 25M, 67EF, 73EF, 84EF and 127EF
  • Concentrations: ~25 μM
  • Incubation Time: 72 hours
  • Method: BTSC spheres are dissociated to single cells with the enzyme Accumax, seeded at 1500 cells/ 96-well and treated with drug or vehicle (DMSO) one day after plating. Cytotoxicity studies are repeated independently using BTSC lines 25M, 67EF, 73EF, 84EF and 127EF. BTSC spheres are dissociated to single cells as above and plated in 96 well plates in triplicate at 3000 cells/ 96-well. In both sets of experiments drugs are used as serial dilutions within the range of 5 μM to 100 nM in the first set and 25 μM to 10 nM. Cell viability following drug treatment is assessed three days later using the alamarBlue assay according to the manufacturer’s instructions. All culture experiments are performed in triplicate with a minimum of three wells per condition.
    (Only for Reference)
Animal Research:[1]
+ Expand
  • Animal Models: NOD-SCID bearing ed with BT73 glioma xenografts
  • Formulation: 10 mg/kg
  • Dosages: Suspended in 50% polyethylene glycol 300 in water
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (163.77 mM)
Ethanol 50 mg/mL warmed (81.88 mM)
Water slightly soluble or insoluble
In vivo Add solvents individually and in order:

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 610.59
Formula

C29H27F5N2O5

CAS No. 1456632-40-8
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

STAT Signaling Pathway Map

Related STAT Products

Tags: buy SH-4-54 | SH-4-54 supplier | purchase SH-4-54 | SH-4-54 cost | SH-4-54 manufacturer | order SH-4-54 | SH-4-54 distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID