Home JAK/STAT STAT inhibitor STAT5-IN-1 For research use only.

STAT5-IN-1

STAT5-IN-1 is a potent and selective STAT5 inhibitor with IC50 of 47 μM for STAT5β isoform.

STAT5-IN-1 Chemical Structure

STAT5-IN-1 Chemical Structure

CAS: 285986-31-4

Selleck's STAT5-IN-1 has been cited by 12 publications

Purity & Quality Control

Batch: Purity: 99.84%
99.84

STAT5-IN-1 Related Products

Signaling Pathway

STAT Signaling Pathways

STAT Signaling Pathway

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Biological Activity

Description STAT5-IN-1 is a potent and selective STAT5 inhibitor with IC50 of 47 μM for STAT5β isoform.
Targets
STAT5β [1]
(Cell-free assay)
47 μM
In vitro
In vitro

STAT5-IN-1, the STAT5 phosphorylation specific inhibitor, selectively blocks the STAT5 signaling and decreases the percentage of CD4+CD25+Foxp3+ Tregs after SEC2 stimulation on murine splenocytes.[2]
Cell Research Cell lines murine splenocytes
Concentrations 0.47 mM
Incubation Time 3 days
Method

Fresh murine splenocytes seeded in 24-well flat-bottom plates are treated with each inhibitor at final concentrations of 10 μM LY294002, 10 mM Trigonelline, 0.47 mM STAT5-IN-1, 50 μM SB431542, and 1 μM SIS3, or treated with 10 μg/mL anti-IL-2 neutralizing antibody, respectively, then cells are stimulated with 104 ng/mL SEC2 for 3 days in a humidified incubator at 37 ℃ with 5% CO2.
In Vivo
In vivo

STAT5-IN-1, a STAT5 inhibitor, significantly attenuates atherosclerosis in ApoE-/- mice induced by HFD via decreasing inflammation.[3]
Animal Research Animal Models ApoE-/- mice
Dosages 10 mg/kg
Administration p.o.

Chemical Information & Solubility

Molecular Weight 293.28 Formula

C16H11N3O3
CAS No. 285986-31-4 SDF --
Smiles C1=CC=C2C(=C1)C(=O)C(=CO2)C=NNC(=O)C3=CN=CC=C3
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 30 mg/mL ( (102.29 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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