Stattic

Catalog No.S7024

Stattic Chemical Structure

Molecular Weight(MW): 211.19

Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1.

Size Price Stock Quantity  
In DMSO USD 90 In stock
USD 110 In stock
USD 170 In stock
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4 Customer Reviews

  • The cell viability was determined in HEC-1B cells treated with MPA, insulin, GSK1904529A or Stattic or with silenced DHCR24.

    Sci Rep, 2017. Stattic purchased from Selleck.

    LN229 cells were treated with vehicle or the small molecule STAT3 inhibitor Stattic and analyzed for cell migration (top panel) or cell invasion (bottom panel) by DAPI staining of nuclei. Bar graphs show quantification of cell motility.

    J Biol Chem 2013 288(8), 5553-61. Stattic purchased from Selleck.

  • (B) Western blotting showing the hepaCAM expression and the phosphorylation level of STAT3, ERK and AKT in cells treated with IL-6 (20 ng/mL) for 24 h with or without the pretreatment of three respective inhibitors, Stattic (10 μM), U0126 (5 μM) and MK-2206 (1 μM)

    Cell Signal, 2017, 32:48-58. Stattic purchased from Selleck.

    Western blot analysis of p-STAT3, total STAT3, ABCA1 and ABCG1 levels in cells pretreated with DMSO or Stattic (10 uM) for 1 hr before and during stimulation with rDKK1 in cells transfected with STAT3 siRNA or negative control, and rDKK1 added into the supernatant.

    FEBS Lett 2014 10.1016/j.febslet.2014.11.023. Stattic purchased from Selleck.

Purity & Quality Control

Choose Selective STAT Inhibitors

Biological Activity

Description Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1.
Features Stattic is the first non-peptide small molecule with inhibitory activity against STAT3 SH2 domain regardless of the STAT3 phosphorylation state in vitro.
Targets
STAT3 [1]
(Cell-free assay)
5.1 μM
In vitro

Stattic inhibits binding of a phosphotyrosine-containing peptide derived from the gp130 receptor to the STAT3 SH2 domain in a strongly temperature-dependent manner. Stattic only has a very weak effect on binding of a tyrosinephosphorylated peptide to the SH2-domain of the tyrosine kinase Lck. And it doesn’t inhibit dimerization of two other dimeric transcription factors (c-Myc/Max and Jun/Jun). It also inhibits fluorescein-labeled phosphopeptides to the SH2 domains of STAT1 and STAT5b. Stattic selectively inhibits DNA binding of STAT3 homodimers at a concentration of 10 μM. Shown to inhibit cellular phosphorylation of STAT3 at Tyr705 with little effect towards STAT1 phosphorylation at Tyr701 (in HepG2 cells) or the phosphorylation of JAK1, JAK2, and c-Src (in MDA-MB-231 and MDA-MB-235S cells). Stattic increases the apoptotic rate of STAT3-dependent breast cancer cell lines. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
H9c2 M2nqfWZ2dmO2aX;uJGF{e2G7 NF7MTmYyOOLCid88US=> NUiwNIhzPOLCiXi= NHWxR4Fz\X[ncoPld{B1cGViZX\m[YN1eyCxZjDJUE0zPw>? NXrOW|M4OjZ|M{m2N|M>
NPC  NVrNXpk{TnWwY4Tpc44hSXO|YYm= M1ywRVAuPy53INM1US=> M4rpZ4Fjd2yrc3jld{BGVVRvbHnr[UBud2ynY4XsZZIh[Wy2ZYLheIlwdnNuIHHu[EBk\WyuIH3p[5JifGmxbjDhcoQhcW64YYPpc44hcW6mdXPl[EBjgSCUS1nQJItvd2OtZH;3ci=> Ml3LNlU6OTV2M{C=
HASMC NX\2b3BKTnWwY4Tpc44hSXO|YYm= NITnTYsyNjJ3LUWg{txO NYW5OVdDOjBibXnu M4jLc2ROW00EoB?= NILvNI9qdmirYnn0d{BxNSi\KT3TWGFVNTFuMzy1JJNq\26jbIRCpC=> MmLrNlU5PDl4MkK=
H9c2  Mo\zSpVv[3Srb36gRZN{[Xl? M1\LS|IwOTBizszN NXrNbJdIOiCq M3rBT2ROW00EoB?= M2exW4Fjem:pYYTld:KhfGinIHP5eI9xem:2ZXP0bZZmKGWoZnXjeJMhd2ZiSVytNlch[WejaX7zeEBUUA>? NYHnRWVCOjV6MkC5NFc>
A431 Mk\GS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3zoUlLDqM7:TR?= MVSyxsBp M2PVV4Jtd2OtczDFS2YuemW4ZYLz[YQh\GWlcnXhd4V{KGmwIHPlcIwhfmmjYnnsbZR6 NWHmfYNZOjV5MkC0N|U>
A431 NH7FSIVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1HsUFLDqM7:TR?= MWOyxsBp NWPmSJRDcW6lcnXhd4V{KGmwIHHwc5B1d3OrczDpcoR2[2WmIHL5JJNpcWuxbnnu NX7acGx4OjV5MkC0N|U>
SiHa Ml;1R4VtdCCYaXHibYxqfHliQYPzZZk> M3PI[|UuPzVibl2= MnrKNlTDqGkEoB?= M2C5fZNpd3e|IH3vdpBpd2yxZ4mgc4Yh[SC2eYDpZ4FtKGGyb4D0c5Rq[yClZXzsJIFv\CCmb4PlMYRmeGWwZHXueEBtd3O|IH;mJINmdGxidnnhZoltcXS7wrC= NYjUdWo1OjV3M{m2OFQ>
SiHa NHTJNnJHfW6ldHnvckBCe3OjeR?= MlzIOU04PSCwTR?= MmjyNlTDqGkEoB?= M4XFNpJm\HWlZYOgeIhmyqCyaH;zdIhwenmuYYTpc44h[XRidHjlJJR6em:|aX7lJJJme2mmdXWgO|A2yqB? M4fpOVI2PTN7NkS0
ECA109 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYewMVIxKM7:TR?= MkXHNlTDqGkEoB?= M3L0SmlEPTB;NT61NEDPxE1? M2noS|I2PDl{NEiw
TE13 M4TxOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYjz[XhMOC1{MDFOwG0> NXvOfFJiOjUEoHlCpC=> MXrJR|UxRTZwMUZCpO69VQ>? MUKyOVQ6OjR6MB?=
KYSE150  MoXCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnvQNE0zOCEQvF2= MV[yOOKhcMLi MX7JR|UxRTF{Lk[0xsDPxE1? NGLseoUzPTR7MkS4NC=>
ECA109 NHfWOpREdG:wb3flcolkKFO3co\peoFtKEG|c3H5 MnPLNE42yqEQvF2= M3XQ[lI1yqCqwrC= MUnzeZBxemW|c3XzJJRp\SClbH;uc4dmdmmlIH\vdo1ifGmxbh?= NELXbHMzPTR7MkS4NC=>
TE13 MmjER4xwdm:pZX7pZ{BUfXK4aY\hcEBCe3OjeR?= NFLEdGgxNjYEoN88US=> Mkf0NlTDqGkEoB?= NEHSb|R{fXCycnXzd4V{KHSqZTDjcI9vd2enbnnjJIZwem2jdHnvci=> MlTPNlU1QTJ2OEC=
KYSE150  MnzTR4xwdm:pZX7pZ{BUfXK4aY\hcEBCe3OjeR?= MXSwMlXDqM7:TR?= M17vTFI1yqCqwrC= MlfQd5VxeHKnc4Pld{B1cGViY3zvco9o\W6rYzDmc5Ju[XSrb36= MmC1NlU1QTJ2OEC=
ECA109 MUjGeY5kfGmxbjDBd5NigQ>? M3HLTVAvPcLizszN M161[FI1yqCqwrC= MX;lcohidmOnczDJVk1qdmS3Y3XkJIdmdmW{YYTpc44hd2ZiRGPCdy=> M36xWVI2PDl{NEiw
PC3M-1E8 M17Ee2Z2dmO2aX;uJGF{e2G7 NVvvOHBFOi53L{WvNVAh|ryP M36we|AuPCCq MlPlbY5pcWKrdIOgeIhmKFOWQWSzJIFkfGm4YYTpc44hcW5iYTDkc5NmNSCjbnSgeIlu\S2mZYDlcoRmdnRibXHucoVzyqB? NVjNN45rOjV{NkGzOlU>
PC3M-1E8 MmnNSpVv[3Srb36gRZN{[Xl? NUjx[WZoOTBizszN MoLpNlQhcA>? M3P5[oRwf26{ZXf1cIF1\XNiQnPsMZhNNCC|dYL2bZZqdiCjbnSgZ{1OgWN? MnnmNlUzPjF|NkW=
PC3M-1E8 MX;GeY5kfGmxbjDBd5NigQ>? NGHWOo4yOCEQvF2= NWnCZnRyOjRiaB?= NXrPd5VmcW6qaXLpeJMhUUxvNjDpcoR2[2WmIGPURXQ{KGGldHn2ZZRqd25iYX7kJJRp\SCLTD22MYlv\HWlZXSgV3RCXDNiYXP0bZZifGmxbh?= MmDLNlUzPjF|NkW=
PC3M-1E8 NVLkOmFMS2yxbn;n[Y5q[yCVdYL2bZZidCCDc4PhfS=> NFOySHgzNjVxNT:xNEDPxE1? MV;pcohq[mm2c9MgeIhmKGOxbH;ufUBnd3KvYYTpc44he2mpbnnmbYNidnSueR?= Mle5NlUzPjF|NkW=
MDA-MB-231 NYrmS|RUTnWwY4Tpc44hSXO|YYm= MmPqNlAh|ryP NFmwZ5kzKGh? NWq0RlBo\XiqaXLpeJMhW26jaXygZY5lKEVvY3HkbIVzcW5iZYjwdoV{e2mxbh?= MUmyOVE2OzN2OR?=
H9c2 MWDGeY5kfGmxbjDBd5NigQ>? MUSyNEDDvU1? MmPmN|AhdWmw NEjyUGFFVVORwrC= NF\QZ4pi[m:uaYPo[ZMheHKxcH;mc4wucW6mdXPl[EBCU1RicHjvd5Bpd3K7bHH0bY9vKGG2IHLveIghe2W{NEezJIFv\CC2aIKzNFg> NF73flczPTFyNUC2Oy=>
HaCaT  M2LQWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1P6dVExKML3TR?= MXKyNEBucW5? MWrEUXNQyqB? NWnPTWhF\W6qYX7j[ZMhe2:{YX\lcoljNSCjbnSgd5VvcXSrbnniMYlv\HWlZXSg[5Jwf3SqIHnubIljcXSrb36= M4LsNlI2ODF|OUC3
Caki-1 NEXBUVFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{XyRlExKML3TR?= NWLaU3FCOjBibXnu MVPEUXNQyqB? NUPJdmZH\W6qYX7j[ZMhe2:{YX\lcoljNSCjbnSgd5VvcXSrbnniMYlv\HWlZXSg[5Jwf3SqIHnubIljcXSrb36= NWq1PY84OjVyMUO5NFc>
HaCaT  NILTdnpCeG:ydH;zbZMhSXO|YYm= M{OyUVExKML3TR?= NVHSVJQ3OjBibXnu NUjtWY96TE2VT9Mg NWGwRnFxcW6lcnXhd4V{KHC{b4DvdpRqd26|IH;mJIFxd3C2b4TpZ{Bk\WyuczDkeYUhfG9idILlZZRu\W62IIfpeIghe2:{YX\lcoljKG:{IIP1col1cW6rYh?= NEG2fo0zPTBzM{mwOy=>
FHL-primed hNSCs MVHD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NEHrZokxNjB{LUWg{txO MmHvO|IhcA>? Mn3lcIVi\HNidH:geIhmKGyxc4Ogc4Yh[2WubDD2bYFjcWyrdImgZZQhcGmpaDDjc45k\W62cnH0bY9v M{fk[|I1QTR3NEO0
ELL-primed hNSCs MWDD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NWO0[445OC5yMj21JO69VQ>? NEnSb5o4OiCq MoHvcIVi\HNidH:geIhmKGyxc4Ogc4Yh[2WubDD2bYFjcWyrdImgZZQhcGmpaDDjc45k\W62cnH0bY9v M172eVI1QTR3NEO0
SS NFrnT3hE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NXjCT21COS1zMDFOwG0> NYLleG1wPzJiaB?= M2TmPGROW09? NIThR2Nk[XW|ZYOgZUBld3OnLXTldIVv\GWwdDDpcohq[mm2aX;uJI9nKHSqZTD2bYFjcWyrdIpCpC=> MXWyOFc2PjFzMR?=
SeAx  Mly1R4VtdCCYaXHibYxqfHliQYPzZZk> NH34N4gyNTFyIN88US=> MVS3NkBp MonHSG1UVw>? M{HtUoNifXOnczDhJIRwe2VvZHXw[Y5l\W62IHnubIljcXSrb36gc4YhfGinII\pZYJqdGm2edMg MU[yOFc2PjFzMR?=
HuT-78 MWrD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MWKxMVExKM7:TR?= MnfnO|IhcA>? NWHxV45XTE2VTx?= NWH1d21t[2G3c3XzJIEh\G:|ZT3k[ZBmdmSnboSgbY5pcWKrdHnvckBw\iC2aHWgeoli[mmuaYT5xsA> MorkNlQ4PTZzMUG=
CD4+ NV7q[4pOSXCxcITvd4l{KEG|c3H5 NW[5NHhnOTEkgJpOwI0> Mnq2NlQhcA>? M4DBU2ROW09? Mn3ybY5lfWOnczDhdI9xfG:|aYOgd5Rzd26pbIm= NFTBc40zPDd3NkGxNS=>
MCF-7 NYnDPZM2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2jFbFAvPDZ7LUOuO|Uh|ryP Ml7YOUBl M2LGN5Jm\HWlZYOgZ4VtdCCwdX3i[ZIhe2mpbnnmbYNidnSueR?= NYnxeIFrOjR5MkiwO|g>
MCF-7/LCC1 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1LXVFAvPDZ7LUOuO|Uh|ryP MmjlOUBl M4HYPJJm\HWlZYOgZ4VtdCCwdX3i[ZIhe2mpbnnmbYNidnSueR?= M2fldFI1PzJ6MEe4
MCF-7/LCC9 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGDK[3IxNjR4OT2zMlc2KM7:TR?= NHHJd242KGR? Mn3SdoVlfWOnczDj[YxtKG63bXLldkB{cWewaX\pZ4FvfGy7 NEXIOIQzPDd{OEC3PC=>
HaCaT MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGW3N3YyOCEEtV2= NE[wOnYzOCCvaX6= M1Hzb2ROW00EoB?= MlzR[Y5p[W6lZYOg[ZZmem:uaX31d{1qdmS3Y3XkJINmdGxiZ4Lve5RpKGmwaHnibZRqd25? Mkf4NlQ1OjNzM{G=
HaCaT MmXSRZBweHSxc3nzJGF{e2G7 MWGxNEDDvU1? NVHVeIQ5OjBibXnu NV\ZPWhRTE2VT9Mg M17tVIVvcGGwY3XzJJRp\SCjcH;weI91cWNiZX\m[YN1eyCxZjDleoVzd2yrbYXz NHHCdpozPDR{M{GzNS=>
MDA-MB-231 NIHVelFHfW6ldHnvckBCe3OjeR?= NGXSRW0yOCEEtV2= NYKxfmh7OjRiaB?= M3LsUWROW00EoB?= NGPxOpFz\WS3Y3XzJHAuW1SDVEOg[ZhxemW|c3nvci=> NIPTb3IzPDN5NkW4Oi=>
SUM-159 NHL0ZZFHfW6ldHnvckBCe3OjeR?= MnLINVAhyrWP MViyOEBp NFrN[ZVFVVORwrC= MX3y[YR2[2W|IGCtV3RCXDNiZYjwdoV{e2mxbh?= M2nxbVI1Ozd4NUi2
SK-BR-3 NHLUcpJHfW6ldHnvckBCe3OjeR?= NV34fXZ7OTBiwsXN MorCNlQhcA>? NXzl[JA2TE2VT9Mg NXjZfI9nemWmdXPld{BRNVOWQWSzJIV5eHKnc4Ppc44> NHnpUnMzPDN5NkW4Oi=>
MCF7-HER2 NFTBUoVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mm[3NE0yOCEQvF2= MofTOFghcA>? MYHEUXNQ NHO0TpNqdmS3Y3XzJINmdGxiZHXheIgh\G:|ZTDk[ZBmdmSnboTsfS=> M1u3Z|I1Ojl5NUC4
MCF7-HER2 MYfGeY5kfGmxbjDBd5NigQ>? Mnn0OUDPxE1? M1PxSlI1KGh? NFTLVZhFVVOR MoXM[IlucW6rc3jld{BUd3hvMjygU4N1NTRuIHHu[EB{dHWpIHX4dJJme3Orb36= MWeyOFI6PzVyOB?=
MCF7-HER2 MonqSpVv[3Srb36gRZN{[Xl? NH7rfIQ2KM7:TR?= MkjRNlQhcA>? M2nYdGROW09? Ml7o[IVkemWjc3XzJJRp\SCneIDy[ZN{cW:wIHzleoVteyCxZjDFUXQhdWG{a3Xyd{whfmmvZX70bY4h[W6mIIPseYc> NEi4VXQzPDJ7N{WwPC=>
MCF7-HER2 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEDsVII2KM7:TR?= M4rLdVI1KGh? NVvX[4tPTE2VTx?= MnvG[Y5p[W6lZYOgZ4VtdCCpcn;3eIghcW6qaXLpeIlwdiClb33ibY5m\CC5aYToJGhmemOncITpci=> MVeyOFI6PzVyOB?=
HMECs  MnPqSpVv[3Srb36gRZN{[Xl? MVixNEDPxE1? NUfjfmJXOiCq NHfmNHdqdmirYnn0d{BKTk8QsdMgcYVlcWG2ZXSgdIhwe3Cqb4L5cIF1cW:wIH;mJHNVSVRzLDDTWGFVOiCjbnSgV3RCXDN? MV:yOFIyOTN{Nx?=
HTR8/SVneo M2LlRmZ2dmO2aX;uJGF{e2G7 NV\KbGVTOcLizszNxsA> MV2xxsBp NWr6fI14e3WycILld5Nm\CCRU12tbY5lfWOnZDDTWGFVOyCyaH;zdIhwenmuYYTpc44> NVvO[Ww6OjRyNkCyOFE>
HTR8/SVneo NInh[lVHfW6ldHnvckBCe3OjeR?= NF6z[XAxNjVxMdMg{txOyqB? MVW0PEBp NYnPdVFpemW|dH;y[ZMhfGinIHX4dJJme3Orb36gc4YhTS2lYXTo[ZJqdiC|dYDwdoV{e2WmIHL5JG9UVQ>? MnX0NlQxPjB{NEG=
HTR8/SVneo NVHxZZhYTnWwY4Tpc44hSXO|YYm= MYqxxsDPxE4EoB?= NI\R[VU1QCCq NVXyfYVFe2mpbnnmbYNidnSueTDpcoNz\WG|ZYOgcYloemG2aX;uJIJ6KE:VTdMg MXiyOFA3ODJ2MR?=
C13* MYHBdI9xfG:|aYOgRZN{[Xl? Mn7NNE0yOCEQvF2= M{XMS|I1NzR6IHi= MnixbY5lfWOnczDhdI9xfG:|aYOgbY4h[SCmb4PlJIFv\CC2aX3lJIRmeGWwZHXueEBu[W6wZYK= Mlf6NlM6PjJ3NUi=
OV2008 M3zRWWFxd3C2b4Ppd{BCe3OjeR?= NX[4cVBUOC1zMDFOwG0> NULGZmxmOjRxNEigbC=> NHLsdlRqdmS3Y3XzJIFxd3C2b4Ppd{BqdiCjIHTvd4Uh[W6mIITpcYUh\GWyZX7k[Y51KG2jbn7ldi=> MnH3NlM6PjJ3NUi=
C13* NYjxbWRiSXCxcITvd4l{KEG|c3H5 M3TVXlI1NzR6IHi= NULPepU5\W6qYX7j[ZMh[2m|cHzheIlvNWmwZIXj[YQh[XCxcITvd4l{ M4K1b|I{QTZ{NUW4
OV2008 NWjaemQySXCxcITvd4l{KEG|c3H5 NGHHNpAzPC92ODDo MmXn[Y5p[W6lZYOgZ4l{eGyjdHnuMYlv\HWlZXSgZZBweHSxc3nz MYOyN|k3OjV3OB?=
W480  M3m3bWZ2dmO2aX;uJGF{e2G7 MUWyMlUwOTBizszN MVmzNEBucW5? NGn2OHRFVVOR MXnz[Y5{cXSrenXzJINmdGy|IITvJINp\W2xcnHkbY91cGW{YYD5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy NIfLbJAzOzl|NEm3Ni=>
SW837 Mn3qSpVv[3Srb36gRZN{[Xl? NYDDcHRoOi53L{GwJO69VQ>? MmH0N|AhdWmw MmL0SG1UVw>? NXXDNmRwe2Wwc3n0bZpmeyClZXzsd{B1dyClaHXtc5Ji\GmxdHjldoFxgSCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? MYeyN|k{PDl5Mh?=
T24 NH:3cpRHfW6ldHnvckBCe3OjeR?= MknNNk8yOC9{MDFOwG0> MXOyOEBp MWHjZZV{\XNiZH;z[U1l\XCnbnTlcpQhcW6qaXLpeIlwdiCxZjD0bIUhS1iFTEGyMYlv\HWlZXSgbY5kemWjc3Wgc4YhcW64YXTpcoch[2WubIO= NVjU[lU6OjN3Mk[wO|k>
CNE1 M4\EfWZ2dmO2aX;uJGF{e2G7 MVOyNEDDvU1? MoC4OFghcA>? MWnicI9kc3NidHjlJGlNNTZiaX7jdoVie2WmIIDoc5NxcG:{eXzheIlwdiCxZjDTeIF1Ow>? Mmn4NlM{QDJ7MUS=
CNE2 NX\KUnBMTnWwY4Tpc44hSXO|YYm= MmPYNlAhyrWP NWizZXM{PDhiaB?= NGPHc3RjdG:la4OgeIhmKEmOLU[gbY5kemWjc3XkJJBpd3OyaH;yfYxifGmxbjDv[kBUfGG2Mx?= Ml;rNlM{QDJ7MUS=
HONE1 M{XU[GZ2dmO2aX;uJGF{e2G7 MmDzNlAhyrWP NF;UWnI1QCCq NXjUOoMy[myxY3vzJJRp\SCLTD22JIlv[3KnYYPl[EBxcG:|cHjvdplt[XSrb36gc4YhW3SjdEO= MXSyN|M5OjlzNB?=
CNE1 NHjoUXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn\WOEDPxE1? Ml\zd4lodmmoaXPhcpRtgSC{ZXT1Z4V{KGOnbHygeoli[mmuaYT5 NGfVWGszOzN6MkmxOC=>
CNE1 MUHGeY5kfGmxbjDBd5NigQ>? MUCwMVIxKM7:TR?= NVLWOJZtOC12IHi= Mm\mbY5pcWKrdIOgV5RifDNiYXP0bZZifGmxbjDpckBiKGSxc3WtJIFv\CC2aX3lMYRmeGWwZHXueEBu[W6wZYK= NInaVmYzOzN6MkmxOC=>
CNE2 M2KxN2Z2dmO2aX;uJGF{e2G7 M3fOOVAuOjBizszN MVqwMVQhcA>? MWTpcohq[mm2czDTeIF1OyCjY4TpeoF1cW:wIHnuJIEh\G:|ZT2gZY5lKHSrbXWt[IVx\W6mZX70JI1idm6nch?= NV2zOFJPOjN|OEK5NVQ>
HONE1 NUDuOY5VTnWwY4Tpc44hSXO|YYm= NHu1T4cxNTJyIN88US=> NV7IbZJKOC12IHi= NW\oOHd6cW6qaXLpeJMhW3SjdEOgZYN1cX[jdHnvckBqdiCjIHTvd4UuKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI> MnywNlM{QDJ7MUS=
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C666-1  MXLD[YxtKF[rYXLpcIl1gSCDc4PhfS=> M{XFSFAvPS14NDFOwG0> MmTlOFghcA>? M1fIZpN2eHC{ZYPz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMUBidmRidHnt[U1l\XCnbnTlcpQhdWGwbnXy NV:wNHlUOjN|OEK5NVQ>
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CT26 MWjGeY5kfGmxbjDBd5NigQ>? NV;IelZHOjBibV2= M4XhPFEhcA>? NUnWeYxVe3WycILld5NmeyCKR1[tbY5lfWOnZDDWSWdHKGW6cILld5Nqd28EoB?= MnzUNlMzOzNzNkO=
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Cal33 M{LmOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYnJR|UxRTJwMkiyJOKyKDBwNEKzJO69VSxiR1m1NF0yNjN2OTFCtUAxNjN4MzFOwG0> M17RNFIzPzdyOEm5
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U-373MG MX3D[YxtKF[rYXLpcIl1gSCDc4PhfS=> M4jlVFAuOTBizszN NHnIboQ4OiCq MmTLSG1UVw>? MmnTbY5pcWKrdIOgZ4VtdCC4aXHibYxqfHliZH;z[UBl\XCnbnTlcpRtgQ>? M2KySlI2PDN4Nkiy
SH-SY5Y NGr3cYhE\WyuIG\pZYJqdGm2eTDBd5NigQ>? Mn20NE0yOCEQvF2= NUm5NWptPzJiaB?= MUjEUXNQ NV;rW|RwcW6qaXLpeJMh[2WubDD2bYFjcWyrdImg[I9{\SCmZYDlcoRmdnSueR?= NEjwN2YzPTR|Nk[4Ni=>
Tu-9648 MljER4VtdCCYaXHibYxqfHliQYPzZZk> NFnPb2QxNTFyIN88US=> M4T4NVczKGh? NVHVVWdHTE2VTx?= NIj5e2xqdmirYnn0d{Bk\WyuII\pZYJqdGm2eTDkc5NmKGSncHXu[IVvfGy7 NYDUdos6OjV2M{[2PFI>
Neuro-2a NITOPGVE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MVqwMVExKM7:TR?= M4S5OlczKGh? NGfHfnpFVVOR MUfpcohq[mm2czDj[YxtKH[rYXLpcIl1gSCmb4PlJIRmeGWwZHXueIx6 NWHlWG9QOjV2M{[2PFI>
PCNs M1PpTmNmdGxiVnnhZoltcXS7IFHzd4F6 M{DlcVAuOTBizszN M13sSFczKGh? NHnyZVlFVVOR M4TDPIlvcGmkaYTzJINmdGxidnnhZoltcXS7IHTvd4Uh\GWyZX7k[Y51dHl? M1H2R|I2PDN4Nkiy
PGCs Mkm5R4VtdCCYaXHibYxqfHliQYPzZZk> M{OzfVAuOTBizszN MlvOO|IhcA>? MUTEUXNQ MVjpcohq[mm2czDj[YxtKH[rYXLpcIl1gSCmb4PlJIRmeGWwZHXueIx6 NGn4bHUzPTR|Nk[4Ni=>
RAW264.7 M1LPNGZ2dmO2aX;uJGF{e2G7 M1n4WFExKM7:TR?= M3jDcVEzKGh? MmOxZYJzd2ejdHXzJJRp\SCvUl7BJIV5eHKnc4Ppc45{KG:oIFrBT|ItKFOWQWSxMEBUXEGWMjygZY5lKFOWQWSzJIlv\HWlZXSgZpkhTE:QIHHu[EBVNTJidH;4bY4> MlTiNlI1PTR2M{G=
RAW264.7 MWTBdI9xfG:|aYOgRZN{[Xl? MmiwOU8yOCEQvF2= NX73W3VnPDVibXnu NVS0bJVK\W6qYX7j[ZMhfG:6aX7zJIlv\HWlZXSgZZBweHSxc3nzJIFv\CCPTWCgcI9{ew>? M2D4bVIzPDV2NEOx
SW480 MXTD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NF7pVFk2NzFyL{KwJO69VQ>? MY[3NkBp NUjnbJp2cW6qaXLpeJMh[2WubDD2bYFjcWyrdImgc4YhfGinIFHMSGgsN0OGMUOzL:Kh[2WubIO= NHPDeGozOTlyMEO5Oy=>
HCT116 NHXnZ5pE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NV7PW3ZkPS9zMD:yNEDPxE1? NVrL[GppPzJiaB?= MlrTbY5pcWKrdIOgZ4VtdCC4aXHibYxqfHlib3[geIhmKEGORFirM2NFOTN|K9MgZ4VtdHN? MnHyNlE6ODB|OUe=
DLD-1  MnXvR4VtdCCYaXHibYxqfHliQYPzZZk> NYXnPJFwPS9zMD:yNEDPxE1? NXHNXpJyPzJiaB?= NFLx[HdqdmirYnn0d{Bk\WyuII\pZYJqdGm2eTDv[kB1cGViQVzETEswS0RzM{OrxsBk\Wyucx?= NYnOb|BDOjF7MECzPVc>
SNU387  NF\5UoZE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MmDHNlDDqM7:TdMg M2DIbVI1KGh? MUDy[YR2[2W|IHPlcIwhfmmjYnnsbZR6 Ml;LNlE{OTF7N{W=
SNU398 NVflUZVjS2WubDDWbYFjcWyrdImgRZN{[Xl? NYfhNJdTOjEEoN88UeKh MoPrNlQhcA>? NFywU3Fz\WS3Y3XzJINmdGxidnnhZoltcXS7 MVOyNVMyOTl5NR?=
HepG2 NH;6ZmxE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NXvKNG9OOjEEoN88UeKh NInqNo4zPCCq M3W4fZJm\HWlZYOgZ4VtdCC4aXHibYxqfHl? Ml\4NlE{OTF7N{W=
Huh-7 NEX2Z|FE\WyuIG\pZYJqdGm2eTDBd5NigQ>? M1TXcFIxyqEQvF5CpC=> M2S4OlI1KGh? NXTLU21iemWmdXPld{Bk\WyuII\pZYJqdGm2eR?= NHHvN5EzOTNzMUm3OS=>
VSMC NWHqRotvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFjk[Io{NzVxMUCg{txO M4CzOVMxKG2rbh?= MYXEUXNQ M4jGcpBz\X[nboTzJHBFT0ZvIHHu[EB1cHKxbXLpck1u\WSrYYTl[EBXW02FIIDyc4xq\mW{YYTpc44hcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> NGToPIQzODh2N{OwOi=>
MDA-MB-231 MV7BdI9xfG:|aYOgRZN{[Xl? M{\teFExKM7:TR?= MnLUNlQhcA>? MkLmSG1UV8Li NHnrfFRqdmS3Y3XzJIFxd3C2b4Ppdy=> NFHPOmUyPzFzNECwOS=>
MDA-MB-435S MX\BdI9xfG:|aYOgRZN{[Xl? M4i4[VExKM7:TR?= MlvGNlQhcA>? MWXEUXNQyqB? MlW5bY5lfWOnczDhdI9xfG:|aYO= MWWxO|EyPDByNR?=

... Click to View More Cell Line Experimental Data

Protocol

Kinase Assay:

[1]

+ Expand

High-Throughput Screening and FluorescencePolarization Assays:

Screening is performed at approximately 30 °C. The specificity ofscreening hits is validated in analogous assays for binding of thetest compounds to the SH2 domains of STAT1, STAT5, and Lck. The final concentration of buffer components used for all FP assays is 10 mM HEPES (pH 7.5), 1 mM EDTA, 0.1% Nonidet P-40, 50 mM NaCl, and 10% DMSO. The absence of dithiothreitol is essential for inhibitory activity. The sequences of the peptides are: STAT3, 5-carboxyfluorescein-GY(PO3H2)LPQTV-NH2; STAT1, 5-carboxyfluorescein-GY(PO3H2)DKPHVL; STAT5, 5-carboxyfluorescein-GY(PO3H2)LVLDKW; and Lck, 5-carboxyfluorescein-GY(PO3H2)EEIP. For specificity analysis at 30 °C, proteins are used at 150 nM (STAT1, STAT3, and STAT5). For specificity analysis at 37 °C, proteins are used at 370 nM (STAT3) or 100 nM (Lck). Proteins are incubated with test compounds in Eppendorf tubes at the indicated temperatures for 60 min prior addition of therespective 5-carboxyfluorescein labeled peptides (final concentration: 10 nM).Before measurement at room temperature, the mixtures are allowed to equilibrate for at least 30 min. Test compounds are used at the indicated concentrations diluted from a 20× stock in DMSO. Binding curves and inhibition curves are fitted with SigmaPlot. All competition curves are repeated three times in independent experiment
Cell Research:

[2]

+ Expand
  • Cell lines: Ly3 cells
  • Concentrations: ~2.5 μM
  • Incubation Time: 48 h
  • Method:

    MTS


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 42 mg/mL (198.87 mM)
Water <1 mg/mL
Ethanol <1 mg/mL
In vivo 2% DMSO+30% PEG 300+H2O 8mg/mL

* 1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 211.19
Formula

C8H5NO4S

CAS No. 19983-44-9
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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STAT Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID