Stattic

Catalog No.S7024

Stattic Chemical Structure

Molecular Weight(MW): 211.19

Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1.

Size Price Stock Quantity  
In DMSO USD 90 In stock
USD 110 In stock
USD 170 In stock

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2 Customer Reviews

  • LN229 cells were treated with vehicle or the small molecule STAT3 inhibitor Stattic and analyzed for cell migration (top panel) or cell invasion (bottom panel) by DAPI staining of nuclei. Bar graphs show quantification of cell motility.

    J Biol Chem 2013 288(8), 5553-61. Stattic purchased from Selleck.

    Western blot analysis of p-STAT3, total STAT3, ABCA1 and ABCG1 levels in cells pretreated with DMSO or Stattic (10 uM) for 1 hr before and during stimulation with rDKK1 in cells transfected with STAT3 siRNA or negative control, and rDKK1 added into the supernatant.

    FEBS Lett 2014 10.1016/j.febslet.2014.11.023. Stattic purchased from Selleck.

Purity & Quality Control

Choose Selective STAT Inhibitors

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Notes:

2. For more details, such as half maximal inhibitory concentrations (IC50s) and working concentrations of each inhibitor, please click on the link of the inhibitor of interest.
3. "+" indicates inhibitory effect. Increased inhibition is marked by a higher "+" designation.
4. Orange "√" refers to compounds which do inhibitory effects on the related isoform, but without specific value.

Biological Activity

Description Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1.
Features Stattic is the first non-peptide small molecule with inhibitory activity against STAT3 SH2 domain regardless of the STAT3 phosphorylation state in vitro.
Targets
STAT3 [1]
(Cell-free assay)
5.1 μM
In vitro

Stattic inhibits binding of a phosphotyrosine-containing peptide derived from the gp130 receptor to the STAT3 SH2 domain in a strongly temperature-dependent manner. Stattic only has a very weak effect on binding of a tyrosinephosphorylated peptide to the SH2-domain of the tyrosine kinase Lck. And it doesn’t inhibit dimerization of two other dimeric transcription factors (c-Myc/Max and Jun/Jun). It also inhibits fluorescein-labeled phosphopeptides to the SH2 domains of STAT1 and STAT5b. Stattic selectively inhibits DNA binding of STAT3 homodimers at a concentration of 10 μM. Shown to inhibit cellular phosphorylation of STAT3 at Tyr705 with little effect towards STAT1 phosphorylation at Tyr701 (in HepG2 cells) or the phosphorylation of JAK1, JAK2, and c-Src (in MDA-MB-231 and MDA-MB-235S cells). Stattic increases the apoptotic rate of STAT3-dependent breast cancer cell lines. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
H9c2 M4PpPGZ2dmO2aX;uJGF{e2G7 NILUeJMyOOLCid88US=> NFfDbYo16oDLaB?= NHzzN3Zz\X[ncoPld{B1cGViZX\m[YN1eyCxZjDJUE0zPw>? NVe2VohPOjZ|M{m2N|M>
NPC  NGC0emxHfW6ldHnvckBCe3OjeR?= NV3lUWtNOC15LkWgxtVO NFHteJdi[m:uaYPo[ZMhTU2WLXzpb4UhdW:uZXP1cIFzKGGudHXyZZRqd26|LDDhcoQh[2WubDDtbYdz[XSrb36gZY5lKGmwdnHzbY9vKGmwZIXj[YQh[nliUlvJVEBsdm:la3Tve44> NHjTWlkzPTlzNUSzNC=>
HASMC NVf5b29KTnWwY4Tpc44hSXO|YYm= MXmxMlI2NTVizszN M3PsSVIxKG2rbh?= MnXJSG1UV8Li MVnpcohq[mm2czDwMUh[MS2VVFHUMVEtOyx3IIPp[45idHQEoB?= MYGyOVg1QTZ{Mh?=
H9c2  MV7GeY5kfGmxbjDBd5NigQ>? NG\JdYwzNzFyIN88US=> NV35SJhUOiCq NH;Ld2NFVVORwrC= NXP0T5o6[WK{b3fheIV{yqC2aHWgZ5l1d3C{b4TlZ5RqfmViZX\m[YN1eyCxZjDJUE0zPyCjZ3HpcpN1KFOK NHnnRVUzPTh{MEmwOy=>
A431 NXWzbm1MT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXnJO5ZSOsLizszN NVq4cJlsOsLiaB?= MV3icI9kc3NiRVfGMZJmfmW{c3XkJIRm[3KnYYPld{BqdiClZXzsJJZq[WKrbHn0fS=> M{KyRVI2PzJyNEO1
A431 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoP2NuKh|ryP NH;6eXIzyqCq MmfwbY5kemWjc3XzJIlvKGGyb4D0c5NqeyCrbnT1Z4VlKGK7IIPobYtwdmmw MnLKNlU4OjB2M{W=
SiHa NVPD[JRHS2WubDDWbYFjcWyrdImgRZN{[Xl? NUL6bphUPS15NTDuUS=> NX72dWxjOjUEoHlCpC=> MoPvd4hwf3NibX;ydIhwdG:peTDv[kBiKHS7cHnjZYwh[XCxcITveIlkKGOnbHygZY5lKGSxc3Wt[IVx\W6mZX70JIxwe3Nib3[gZ4VtdCC4aXHibYxqfHoEoB?= NWP1NW5IOjV3M{m2OFQ>
SiHa MVTGeY5kfGmxbjDBd5NigQ>? NGWzcG02NTd3IH7N NUDTW3FROjUEoHlCpC=> NX;rPFBEemWmdXPld{B1cGYEoIDoc5NxcG:{eXzheIlwdiCjdDD0bIUhfHm{b4PpcoUhemW|aXT1[UA4ODYEoB?= NYXHXZpYOjV3M{m2OFQ>
ECA109 M3zjVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX;SW4RDOC1{MDFOwG0> NH\le3ozPMLiaNMg MXTJR|UxRTVwNUCg{txO NYnmc|RKOjV2OUK0PFA>
TE13 M4XrWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWfyPZdDOC1{MDFOwG0> MUCyOOKhcMLi M2j1bmlEPTB;Nj6xOeKh|ryP NWTqU2FqOjV2OUK0PFA>
KYSE150  NVjVO|hXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWmyS2tkOC1{MDFOwG0> MYKyOOKhcMLi MVLJR|UxRTF{Lk[0xsDPxE1? NWPaT215OjV2OUK0PFA>
ECA109 MkHSR4xwdm:pZX7pZ{BUfXK4aY\hcEBCe3OjeR?= MViwMlXDqM7:TR?= NUfDfYdbOjUEoHlCpC=> M3;SZpN2eHC{ZYPz[ZMhfGinIHPsc45w\2WwaXOg[o9zdWG2aX;u MVeyOVQ6OjR6MB?=
TE13 NIPkeGJEdG:wb3flcolkKFO3co\peoFtKEG|c3H5 MlTPNE42yqEQvF2= NYr2cZhvOjUEoHlCpC=> Mljkd5VxeHKnc4Pld{B1cGViY3zvco9o\W6rYzDmc5Ju[XSrb36= NUXCR2NpOjV2OUK0PFA>
KYSE150  MkXyR4xwdm:pZX7pZ{BUfXK4aY\hcEBCe3OjeR?= NEXleYgxNjYEoN88US=> MnK0NlTDqGkEoB?= NX\EfI1Ue3WycILld5NmeyC2aHWgZ4xwdm:pZX7pZ{Bnd3KvYYTpc44> MnLjNlU1QTJ2OEC=
ECA109 NGTUTHRHfW6ldHnvckBCe3OjeR?= NEHFNFcxNjYEoN88US=> NUWxcGMyOjUEoHlCpC=> M1\jdoVvcGGwY3XzJGlTNWmwZIXj[YQh\2WwZYLheIlwdiCxZjDEV2J{ NULGPJVzOjV2OUK0PFA>
PC3M-1E8 M2HmdWZ2dmO2aX;uJGF{e2G7 Ml\JNk42NzVxMUCg{txO NVTOS|dLOC12IHi= Mnz4bY5pcWKrdIOgeIhmKFOWQWSzJIFkfGm4YYTpc44hcW5iYTDkc5NmNSCjbnSgeIlu\S2mZYDlcoRmdnRibXHucoVzyqB? MXGyOVI3OTN4NR?=
PC3M-1E8 M2nKcmZ2dmO2aX;uJGF{e2G7 NXPLd3RIOTBizszN NIDkcHUzPCCq MkLB[I94dnKnZ4XsZZRmeyCEY3ytfGwtKHO3co\peolvKGGwZDDjMW16[w>? MkiyNlUzPjF|NkW=
PC3M-1E8 NIPH[HFHfW6ldHnvckBCe3OjeR?= Mn7HNVAh|ryP NUTTc|dHOjRiaB?= NYXheZR7cW6qaXLpeJMhUUxvNjDpcoR2[2WmIGPURXQ{KGGldHn2ZZRqd25iYX7kJJRp\SCLTD22MYlv\HWlZXSgV3RCXDNiYXP0bZZifGmxbh?= NIjjbXkzPTJ4MUO2OS=>
PC3M-1E8 MnrUR4xwdm:pZX7pZ{BUfXK4aY\hcEBCe3OjeR?= MkjaNk42NzVxMUCg{txO MYjpcohq[mm2c9MgeIhmKGOxbH;ufUBnd3KvYYTpc44he2mpbnnmbYNidnSueR?= NXH4bXZ7OjV{NkGzOlU>
MDA-MB-231 MoTrSpVv[3Srb36gRZN{[Xl? M1nWTlIxKM7:TR?= NYrnS|czOiCq MXvlfIhq[mm2czDTcoFqdCCjbnSgSU1k[WSqZYLpckBmgHC{ZYPzbY9v NF;Re|czPTF3M{O0PS=>
H9c2 NIHoZWtHfW6ldHnvckBCe3OjeR?= NF;lXmozOCEEtV2= MV:zNEBucW5? M3nHZWROW00EoB?= NHTHVm9i[m:uaYPo[ZMheHKxcH;mc4wucW6mdXPl[EBCU1RicHjvd5Bpd3K7bHH0bY9vKGG2IHLveIghe2W{NEezJIFv\CC2aIKzNFg> MnvpNlUyODVyNke=
HaCaT  NFPoSndIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M37NU|ExKML3TR?= NET3OnEzOCCvaX6= NVHtRZJHTE2VT9Mg M4PnUoVvcGGwY3XzJJNwemGoZX7pZk0h[W6mIIP1col1cW6rYj3pcoR2[2WmIHfyc5d1cCCrbnjpZol1cW:w NVzCbVhkOjVyMUO5NFc>
Caki-1 M{KxRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUDBbZk5OTBiwsXN MlH4NlAhdWmw NIjUR4RFVVORwrC= M1vmXIVvcGGwY3XzJJNwemGoZX7pZk0h[W6mIIP1col1cW6rYj3pcoR2[2WmIHfyc5d1cCCrbnjpZol1cW:w MkfYNlUxOTN7MEe=
HaCaT  MX7BdI9xfG:|aYOgRZN{[Xl? MmPCNVAhyrWP NWfzPZFYOjBibXnu NUK2bHBVTE2VT9Mg NXfSUFZQcW6lcnXhd4V{KHC{b4DvdpRqd26|IH;mJIFxd3C2b4TpZ{Bk\WyuczDkeYUhfG9idILlZZRu\W62IIfpeIghe2:{YX\lcoljKG:{IIP1col1cW6rYh?= M4jnWVI2ODF|OUC3
FHL-primed hNSCs M2S1S2NmdGxiVnnhZoltcXS7IFHzd4F6 M4jGcFAvODJvNTFOwG0> Ml\YO|IhcA>? NYrhXGtIdGWjZIOgeI8hfGinIHzvd5Mhd2ZiY3XscEB3cWGkaXzpeJkh[XRiaHnnbEBkd26lZX70doF1cW:w MXSyOFk1PTR|NB?=
ELL-primed hNSCs MmrNR4VtdCCYaXHibYxqfHliQYPzZZk> NIHybWMxNjB{LUWg{txO NX3vS5A{PzJiaB?= Ml3jcIVi\HNidH:geIhmKGyxc4Ogc4Yh[2WubDD2bYFjcWyrdImgZZQhcGmpaDDjc45k\W62cnH0bY9v NInPbYYzPDl2NUSzOC=>
SS MXzD[YxtKF[rYXLpcIl1gSCDc4PhfS=> M2O5bVEuOTBizszN MkTOO|IhcA>? MnjNSG1UVw>? NXLZcoV2[2G3c3XzJIEh\G:|ZT3k[ZBmdmSnboSgbY5pcWKrdHnvckBw\iC2aHWgeoli[mmuaYT5xsA> MYiyOFc2PjFzMR?=
SeAx  M37HT2NmdGxiVnnhZoltcXS7IFHzd4F6 NGixcmwyNTFyIN88US=> M{TxeVczKGh? NX3LbYxITE2VTx?= M1PTWoNifXOnczDhJIRwe2VvZHXw[Y5l\W62IHnubIljcXSrb36gc4YhfGinII\pZYJqdGm2edMg NEj1SmIzPDd3NkGxNS=>
HuT-78 M3\RW2NmdGxiVnnhZoltcXS7IFHzd4F6 MnvDNU0yOCEQvF2= MmLRO|IhcA>? M3LMNWROW09? M1LzWYNifXOnczDhJIRwe2VvZHXw[Y5l\W62IHnubIljcXSrb36gc4YhfGinII\pZYJqdGm2edMg MYWyOFc2PjFzMR?=
CD4+ MXnBdI9xfG:|aYOgRZN{[Xl? MlTRNVDjiIoQvH2= NYHNO4k1OjRiaB?= MV\EUXNQ NHqzflVqdmS3Y3XzJIFxd3C2b4Ppd{B{fHKxbnfsfS=> MmLWNlQ4PTZzMUG=
MCF-7 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3LhbFAvPDZ7LUOuO|Uh|ryP MVO1JIQ> M1HJZpJm\HWlZYOgZ4VtdCCwdX3i[ZIhe2mpbnnmbYNidnSueR?= MmPiNlQ4OjhyN{i=
MCF-7/LCC1 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYewMlQ3QS1|Lke1JO69VQ>? NHXwVXA2KGR? M4S0bJJm\HWlZYOgZ4VtdCCwdX3i[ZIhe2mpbnnmbYNidnSueR?= NXfndGIxOjR5MkiwO|g>
MCF-7/LCC9 NICxR2FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXrhXWdWOC52NkmtN{44PSEQvF2= NVvEZ4J2PSCm MlXmdoVlfWOnczDj[YxtKG63bXLldkB{cWewaX\pZ4FvfGy7 MnzwNlQ4OjhyN{i=
HaCaT M1XPS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2O4WFExKML3TR?= NFrleZUzOCCvaX6= MYLEUXNQyqB? MmPX[Y5p[W6lZYOg[ZZmem:uaX31d{1qdmS3Y3XkJINmdGxiZ4Lve5RpKGmwaHnibZRqd25? MVKyOFQzOzF|MR?=
HaCaT NFnyUmVCeG:ydH;zbZMhSXO|YYm= M4DmTlExKML3TR?= NHPzeHczOCCvaX6= NX7vPHR5TE2VT9Mg NYHjeHVN\W6qYX7j[ZMhfGinIHHwc5B1d3SrYzDl[oZm[3S|IH;mJIV3\XKxbHnteZM> M{\JS|I1PDJ|MUOx
MDA-MB-231 MkjqSpVv[3Srb36gRZN{[Xl? NUDUR4hXOTBiwsXN MlvQNlQhcA>? NUjMO4xvTE2VT9Mg MmLjdoVlfWOnczDQMXNVSVR|IHX4dJJme3Orb36= MYGyOFM4PjV6Nh?=
SUM-159 MoTZSpVv[3Srb36gRZN{[Xl? M33TXFExKML3TR?= NUD5TndoOjRiaB?= M3iySWROW00EoB?= MlPXdoVlfWOnczDQMXNVSVR|IHX4dJJme3Orb36= MWeyOFM4PjV6Nh?=
SK-BR-3 MWTGeY5kfGmxbjDBd5NigQ>? NX7EfW5sOTBiwsXN NVnWN4Z5OjRiaB?= NXi1S|JOTE2VT9Mg M4HE[JJm\HWlZYOgVE1UXEGWMzDlfJBz\XO|aX;u NEPFRnczPDN5NkW4Oi=>
MCF7-HER2 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUKwMVExKM7:TR?= M1rGV|Q5KGh? Ml3BSG1UVw>? M1;YPYlv\HWlZYOgZ4VtdCCmZXH0bEBld3OnIHTldIVv\GWwdHz5 MUSyOFI6PzVyOB?=
MCF7-HER2 M1PqUmZ2dmO2aX;uJGF{e2G7 NYXwcYdNPSEQvF2= M3HYOFI1KGh? MYjEUXNQ NX3ER3ZV\GmvaX7pd4hmeyCVb4itNkwhV2O2LUSsJIFv\CC|bIXnJIV5eHKnc4Ppc44> MkHpNlQzQTd3MEi=
MCF7-HER2 M3;QT2Z2dmO2aX;uJGF{e2G7 NYCyW4lOPSEQvF2= NV63c3p1OjRiaB?= MVvEUXNQ MkHG[IVkemWjc3XzJJRp\SCneIDy[ZN{cW:wIHzleoVteyCxZjDFUXQhdWG{a3Xyd{whfmmvZX70bY4h[W6mIIPseYc> MnSyNlQzQTd3MEi=
MCF7-HER2 NUO2eoFiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2L5SFUh|ryP MkDHNlQhcA>? MYjEUXNQ MXLlcohidmOnczDj[YxtKGe{b4f0bEBqdmirYnn0bY9vKGOxbXLpcoVlKHerdHigTIVz[2WydHnu NVLwUWUyOjR{OUe1NFg>
HMECs  M2f3bmZ2dmO2aX;uJGF{e2G7 MoTnNVAh|ryP NYHtdW9YOiCq NGnqbZhqdmirYnn0d{BKTk8QsdMgcYVlcWG2ZXSgdIhwe3Cqb4L5cIF1cW:wIH;mJHNVSVRzLDDTWGFVOiCjbnSgV3RCXDN? M1i4UlI1OjFzM{K3
HTR8/SVneo MlP5SpVv[3Srb36gRZN{[Xl? NGLhSoQyyqEQvF5CpC=> MUGxxsBp M{CzdpN2eHC{ZYPz[YQhV1OPLXnu[JVk\WRiU2TBWFMheGixc4Doc5J6dGG2aX;u MXyyOFA3ODJ2MR?=
HTR8/SVneo NXmwUlFYTnWwY4Tpc44hSXO|YYm= NEfOW5kxNjVxMdMg{txOyqB? MUG0PEBp M2[4WJJme3SxcnXzJJRp\SCneIDy[ZN{cW:wIH;mJGUu[2GmaHXybY4he3WycILld5Nm\CCkeTDPV20> NUS0enRHOjRyNkCyOFE>
HTR8/SVneo MXTGeY5kfGmxbjDBd5NigQ>? M{fkbFHDqM7:TdMg MWK0PEBp NVfVfJFue2mpbnnmbYNidnSueTDpcoNz\WG|ZYOgcYloemG2aX;uJIJ6KE:VTdMg MUiyOFA3ODJ2MR?=
C13* NWrKN2ttSXCxcITvd4l{KEG|c3H5 MX[wMVExKM7:TR?= NIjJeHMzPC92ODDo NHfWNI9qdmS3Y3XzJIFxd3C2b4Ppd{BqdiCjIHTvd4Uh[W6mIITpcYUh\GWyZX7k[Y51KG2jbn7ldi=> MorHNlM6PjJ3NUi=
OV2008 MXjBdI9xfG:|aYOgRZN{[Xl? NGjofIwxNTFyIN88US=> MlPlNlQwPDhiaB?= NVzjcHVbcW6mdXPld{BieG:ydH;zbZMhcW5iYTDkc5NmKGGwZDD0bY1mKGSncHXu[IVvfCCvYX7u[ZI> M3PkVlI{QTZ{NUW4
C13* NHK1PZdCeG:ydH;zbZMhSXO|YYm= MWKyOE81QCCq MlHX[Y5p[W6lZYOgZ4l{eGyjdHnuMYlv\HWlZXSgZZBweHSxc3nz MoLENlM6PjJ3NUi=
OV2008 MYPBdI9xfG:|aYOgRZN{[Xl? NGLqem4zPC92ODDo Mlv5[Y5p[W6lZYOgZ4l{eGyjdHnuMYlv\HWlZXSgZZBweHSxc3nz NILqZWQzOzl4MkW1PC=>
W480  MkTHSpVv[3Srb36gRZN{[Xl? MUiyMlUwOTBizszN M1v6TVMxKG2rbh?= M{TUdGROW09? NHGwPGd{\W6|aYTpfoV{KGOnbHzzJJRwKGOqZX3vdoFlcW:2aHXyZZB6KGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz NEDJbGMzOzl|NEm3Ni=>
SW837 M4q2VmZ2dmO2aX;uJGF{e2G7 NU\BTpNuOi53L{GwJO69VQ>? NXLRc3dDOzBibXnu NYX0VodqTE2VTx?= MWjz[Y5{cXSrenXzJINmdGy|IITvJINp\W2xcnHkbY91cGW{YYD5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy NWDYNIRTOjN7M{S5O|I>
T24 NVy3VZNHTnWwY4Tpc44hSXO|YYm= NULnSmV[Oi9zMD:yNEDPxE1? NYfWe|VQOjRiaB?= MmfqZ4F2e2W|IHTvd4Uu\GWyZX7k[Y51KGmwaHnibZRqd25ib3[geIhmKEO[Q1yxNk1qdmS3Y3XkJIlv[3KnYYPlJI9nKGmwdnHkbY5oKGOnbHzz MmLUNlM2OjZyN{m=
CNE1 Mm[zSpVv[3Srb36gRZN{[Xl? MUCyNEDDvU1? NIXSWXc1QCCq NFXkXmljdG:la4OgeIhmKEmOLU[gbY5kemWjc3XkJJBpd3OyaH;yfYxifGmxbjDv[kBUfGG2Mx?= NVnERpJLOjN|OEK5NVQ>
CNE2 MUfGeY5kfGmxbjDBd5NigQ>? NWTJO3ZbOjBiwsXN MorhOFghcA>? MX3icI9kc3NidHjlJGlNNTZiaX7jdoVie2WmIIDoc5NxcG:{eXzheIlwdiCxZjDTeIF1Ow>? MVOyN|M5OjlzNB?=
HONE1 MkLtSpVv[3Srb36gRZN{[Xl? M{K1eFIxKML3TR?= MnyzOFghcA>? MUXicI9kc3NidHjlJGlNNTZiaX7jdoVie2WmIIDoc5NxcG:{eXzheIlwdiCxZjDTeIF1Ow>? MmHHNlM{QDJ7MUS=
CNE1 M2PCS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4\LVFQh|ryP Mo[3d4lodmmoaXPhcpRtgSC{ZXT1Z4V{KGOnbHygeoli[mmuaYT5 MXqyN|M5OjlzNB?=
CNE1 M3i3W2Z2dmO2aX;uJGF{e2G7 MVewMVIxKM7:TR?= NX\DN4VCOC12IHi= MULpcohq[mm2czDTeIF1OyCjY4TpeoF1cW:wIHnuJIEh\G:|ZT2gZY5lKHSrbXWt[IVx\W6mZX70JI1idm6nch?= MWiyN|M5OjlzNB?=
CNE2 MXTGeY5kfGmxbjDBd5NigQ>? MVKwMVIxKM7:TR?= NFjGVZExNTRiaB?= M1P0e4lvcGmkaYTzJHN1[XR|IHHjeIl3[XSrb36gbY4h[SCmb4PlMUBidmRidHnt[U1l\XCnbnTlcpQhdWGwbnXy NHTDWVgzOzN6MkmxOC=>
HONE1 M3PGe2Z2dmO2aX;uJGF{e2G7 M1TPcVAuOjBizszN NFnsdpIxNTRiaB?= M1vSbolvcGmkaYTzJHN1[XR|IHHjeIl3[XSrb36gbY4h[SCmb4PlMUBidmRidHnt[U1l\XCnbnTlcpQhdWGwbnXy NX3X[oJVOjN|OEK5NVQ>
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PGCs MWLD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MmnrNE0yOCEQvF2= NF3RTY44OiCq MUXEUXNQ NUP0dolTcW6qaXLpeJMh[2WubDD2bYFjcWyrdImg[I9{\SCmZYDlcoRmdnSueR?= M2j2b|I2PDN4Nkiy
RAW264.7 MkPsSpVv[3Srb36gRZN{[Xl? M120UVExKM7:TR?= MUGxNkBp NHPqboJi[nKxZ3H0[ZMhfGinIH3SUmEh\XiycnXzd4lwdnNib3[gTmFMOixiU2TBWFEtKFOWQWSyMEBidmRiU2TBWFMhcW6mdXPl[EBjgSCGT16gZY5lKFRvMjD0c5hqdg>? NHj6[HIzOjR3NESzNS=>
RAW264.7 M3rFbGFxd3C2b4Ppd{BCe3OjeR?= M2XuWlUwOTBizszN NU\UbYwzPDVibXnu MWHlcohidmOnczD0c5hqdnNiaX7keYNm\CCjcH;weI9{cXNiYX7kJG1OWCCub4Pz NFHjVWwzOjR3NESzNS=>
SW480 MkWwR4VtdCCYaXHibYxqfHliQYPzZZk> NIPvUmE2NzFyL{KwJO69VQ>? MkDYO|IhcA>? M{XSUYlvcGmkaYTzJINmdGxidnnhZoltcXS7IH;mJJRp\SCDTFTIL{9ETDF|MzxCpINmdGy| NXfxOm1LOjF7MECzPVc>
HCT116 M{jyU2NmdGxiVnnhZoltcXS7IFHzd4F6 MW[1M|ExNzJyIN88US=> M3TI[FczKGh? MoizbY5pcWKrdIOgZ4VtdCC4aXHibYxqfHlib3[geIhmKEGORFirM2NFOTN|K9MgZ4VtdHN? MWWyNVkxODN7Nx?=
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SNU387  MX\D[YxtKF[rYXLpcIl1gSCDc4PhfS=> MV:yNOKh|ryPwrC= NHPFT40zPCCq NIjsWHhz\WS3Y3XzJINmdGxidnnhZoltcXS7 MWKyNVMyOTl5NR?=
SNU398 NH7vcIRE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MVqyNOKh|ryPwrC= Ml3wNlQhcA>? NHPEcYtz\WS3Y3XzJINmdGxidnnhZoltcXS7 M1[1[lIyOzFzOUe1
HepG2 M1\pU2NmdGxiVnnhZoltcXS7IFHzd4F6 NIDUNY0zOMLizszNxsA> Ml3INlQhcA>? MUPy[YR2[2W|IHPlcIwhfmmjYnnsbZR6 M13qT|IyOzFzOUe1
Huh-7 M1zLUWNmdGxiVnnhZoltcXS7IFHzd4F6 M4XKNFIxyqEQvF5CpC=> MWSyOEBp MXHy[YR2[2W|IHPlcIwhfmmjYnnsbZR6 NVP4WJF5OjF|MUG5O|U>
VSMC M3\oXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4ewUFMwPS9zMDFOwG0> NIfJPXY{OCCvaX6= NVPDW2M1TE2VTx?= NVy1OoV6eHKndnXueJMhWESJRj2gZY5lKHSqcn;tZolvNW2nZHnheIVlKF[VTVOgdJJwdGmoZYLheIlwdiCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? M4HqfVIxQDR5M{C2
MDA-MB-231 NF7oXmFCeG:ydH;zbZMhSXO|YYm= M3PCXVExKM7:TR?= M1SybFI1KGh? MV3EUXNQyqB? MXPpcoR2[2W|IHHwc5B1d3Orcx?= M1roT|E4OTF2MEC1
MDA-MB-435S MmfQRZBweHSxc3nzJGF{e2G7 NVftfJhIOTBizszN MmXWNlQhcA>? NWfKXmF6TE2VT9Mg M2SwfYlv\HWlZYOgZZBweHSxc3nz NFfEN5MyPzFzNECwOS=>

... Click to View More Cell Line Experimental Data

Protocol

Kinase Assay:[1]
+ Expand

High-Throughput Screening and Fluorescence Polarization Assays :

Screening is performed at approximately 30 °C. The specificity of screening hits is validated in analogous assays for binding of the test compounds to the SH2 domains of STAT1, STAT5, and Lck. The final concentration of buffer components used for all FP assays is 10 mM HEPES (pH 7.5), 1 mM EDTA, 0.1% Nonidet P-40, 50 mM NaCl, and 10% DMSO. The absence of dithiothreitol is essential for inhibitory activity. The sequences of the peptides are: STAT3, 5-carboxyfluorescein-GY(PO3H2)LPQTV-NH2; STAT1, 5-carboxyfluorescein-GY(PO3H2)DKPHVL; STAT5, 5-carboxyfluorescein-GY(PO3H2)LVLDKW; and Lck, 5-carboxyfluorescein-GY(PO3H2)EEIP. For specificity analysis at 30 °C, proteins are used at 150 nM (STAT1, STAT3, and STAT5). For specificity analysis at 37 °C, proteins are used at 370 nM (STAT3) or 100 nM (Lck). Proteins are incubated with test compounds in Eppendorf tubes at the indicated temperatures for 60 min prior addition of the respective 5-carboxyfluorescein labeled peptides (final concentration: 10 nM). Before measurement at room temperature, the mixtures are allowed to equilibrate for at least 30 min. Test compounds are used at the indicated concentrations diluted from a 20× stock in DMSO. Binding curves and inhibition curves are fitted with SigmaPlot. All competition curves are repeated three times in independent experiment
Cell Research:[2]
+ Expand
  • Cell lines: Ly3 cells
  • Concentrations: ~2.5 μM
  • Incubation Time: 48 h
  • Method: MTS
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 42 mg/mL (198.87 mM)
Water <1 mg/mL
Ethanol <1 mg/mL
In vivo 2% DMSO+30% PEG 300+H2O 8mg/mL

* 1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 211.19
Formula

C8H5NO4S

CAS No. 19983-44-9
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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STAT Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID