Stattic

Catalog No.S7024

Stattic Chemical Structure

Molecular Weight(MW): 211.19

Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1.

Size Price Stock Quantity  
In DMSO USD 90 In stock
USD 110 In stock
USD 170 In stock
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4 Customer Reviews

  • The cell viability was determined in HEC-1B cells treated with MPA, insulin, GSK1904529A or Stattic or with silenced DHCR24.

    Sci Rep, 2017. Stattic purchased from Selleck.

    LN229 cells were treated with vehicle or the small molecule STAT3 inhibitor Stattic and analyzed for cell migration (top panel) or cell invasion (bottom panel) by DAPI staining of nuclei. Bar graphs show quantification of cell motility.

    J Biol Chem 2013 288(8), 5553-61. Stattic purchased from Selleck.

  • (B) Western blotting showing the hepaCAM expression and the phosphorylation level of STAT3, ERK and AKT in cells treated with IL-6 (20 ng/mL) for 24 h with or without the pretreatment of three respective inhibitors, Stattic (10 μM), U0126 (5 μM) and MK-2206 (1 μM)

    Cell Signal, 2017, 32:48-58. Stattic purchased from Selleck.

    Western blot analysis of p-STAT3, total STAT3, ABCA1 and ABCG1 levels in cells pretreated with DMSO or Stattic (10 uM) for 1 hr before and during stimulation with rDKK1 in cells transfected with STAT3 siRNA or negative control, and rDKK1 added into the supernatant.

    FEBS Lett 2014 10.1016/j.febslet.2014.11.023. Stattic purchased from Selleck.

Purity & Quality Control

Choose Selective STAT Inhibitors

Biological Activity

Description Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1.
Features Stattic is the first non-peptide small molecule with inhibitory activity against STAT3 SH2 domain regardless of the STAT3 phosphorylation state in vitro.
Targets
STAT3 [1]
(Cell-free assay)
5.1 μM
In vitro

Stattic inhibits binding of a phosphotyrosine-containing peptide derived from the gp130 receptor to the STAT3 SH2 domain in a strongly temperature-dependent manner. Stattic only has a very weak effect on binding of a tyrosinephosphorylated peptide to the SH2-domain of the tyrosine kinase Lck. And it doesn’t inhibit dimerization of two other dimeric transcription factors (c-Myc/Max and Jun/Jun). It also inhibits fluorescein-labeled phosphopeptides to the SH2 domains of STAT1 and STAT5b. Stattic selectively inhibits DNA binding of STAT3 homodimers at a concentration of 10 μM. Shown to inhibit cellular phosphorylation of STAT3 at Tyr705 with little effect towards STAT1 phosphorylation at Tyr701 (in HepG2 cells) or the phosphorylation of JAK1, JAK2, and c-Src (in MDA-MB-231 and MDA-MB-235S cells). Stattic increases the apoptotic rate of STAT3-dependent breast cancer cell lines. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
H9c2 MV3GeY5kfGmxbjDBd5NigQ>? NHmyPYIyOOLCid88US=> Mn;4OQKBkWh? NYDwPI81emW4ZYLz[ZMhfGinIHXm[oVkfHNib3[gTWwuOjd? M2fjflI3OzN7NkOz
NPC  M2ixb2Z2dmO2aX;uJGF{e2G7 NX\xb5NEOC15LkWgxtVO MU\hZo9tcXOqZYOgSW1VNWyra3WgcY9t\WO3bHHyJIFtfGW{YYTpc45{NCCjbnSgZ4VtdCCvaXfyZZRqd25iYX7kJIlvfmG|aX;uJIlv\HWlZXSgZpkhWkuLUDDrco9kc2Sxd36= MnHpNlU6OTV2M{C=
HASMC Mmq0SpVv[3Srb36gRZN{[Xl? MVuxMlI2NTVizszN M37KTVIxKG2rbh?= MlnOSG1UV8Li MWrpcohq[mm2czDwMUh[MS2VVFHUMVEtOyx3IIPp[45idHQEoB?= Mn7NNlU5PDl4MkK=
H9c2  NFnIdHNHfW6ldHnvckBCe3OjeR?= NUfUPHBbOi9zMDFOwG0> Mn3PNkBp NWTkOndPTE2VT9Mg MnrzZYJzd2ejdHXzxsB1cGViY4n0c5Bzd3SnY4TpeoUh\W[oZXP0d{Bw\iCLTD2yO{Bi\2GrboP0JHNJ M2LKXVI2QDJyOUC3
A431 NIjH[VJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGrjXm4zyqEQvF2= NUTV[YZsOsLiaB?= MX7icI9kc3NiRVfGMZJmfmW{c3XkJIRm[3KnYYPld{BqdiClZXzsJJZq[WKrbHn0fS=> MYeyOVczODR|NR?=
A431 M1LGSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFXUc5kzyqEQvF2= MXmyxsBp NE\sb4ZqdmO{ZXHz[ZMhcW5iYYDvdJRwe2m|IHnu[JVk\WRiYomgd4hqc2:waX6= M4fCU|I2PzJyNEO1
SiHa M{\Ze2NmdGxiVnnhZoltcXS7IFHzd4F6 NUG3[HdIPS15NTDuUS=> NH3EPI0zPMLiaNMg M2HZfZNpd3e|IH3vdpBpd2yxZ4mgc4Yh[SC2eYDpZ4FtKGGyb4D0c5Rq[yClZXzsJIFv\CCmb4PlMYRmeGWwZHXueEBtd3O|IH;mJINmdGxidnnhZoltcXS7wrC= M4\pSVI2PTN7NkS0
SiHa NV;Rd2NHTnWwY4Tpc44hSXO|YYm= MXy1MVc2KG6P M4PPd|I1yqCqwrC= NETXUZpz\WS3Y3XzJJRp\cLicHjvd5Bpd3K7bHH0bY9vKGG2IITo[UB1gXKxc3nu[UBz\XOrZIXlJFcxPcLi Mn3pNlU2Ozl4NES=
ECA109 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYiwMVIxKM7:TR?= M1rIV|I1yqCqwrC= MkP1TWM2OD13LkWwJO69VQ>? MUOyOVQ6OjR6MB?=
TE13 NGPzSGNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmrDNE0zOCEQvF2= NUnBTnY6OjUEoHlCpC=> M4\wcmlEPTB;Nj6xOeKh|ryP MWmyOVQ6OjR6MB?=
KYSE150  NGTS[nFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MofUNE0zOCEQvF2= NHLq[YQzPMLiaNMg NGnTcWtKSzVyPUGyMlY1yqEQvF2= NHHSWnUzPTR7MkS4NC=>
ECA109 NFTnR4NEdG:wb3flcolkKFO3co\peoFtKEG|c3H5 MV2wMlXDqM7:TR?= M3[xO|I1yqCqwrC= NGT3RVZ{fXCycnXzd4V{KHSqZTDjcI9vd2enbnnjJIZwem2jdHnvci=> MWOyOVQ6OjR6MB?=
TE13 MUXDcI9vd2enbnnjJHN2en[rdnHsJGF{e2G7 M375O|AvPcLizszN MoHMNlTDqGkEoB?= M{Tyd5N2eHC{ZYPz[ZMhfGinIHPsc45w\2WwaXOg[o9zdWG2aX;u NEL6WYUzPTR7MkS4NC=>
KYSE150  NYXUbXZ5S2yxbn;n[Y5q[yCVdYL2bZZidCCDc4PhfS=> MV:wMlXDqM7:TR?= M4LF[|I1yqCqwrC= NIPQd2N{fXCycnXzd4V{KHSqZTDjcI9vd2enbnnjJIZwem2jdHnvci=> MUWyOVQ6OjR6MB?=
ECA109 NYnYXG1{TnWwY4Tpc44hSXO|YYm= MoPENE42yqEQvF2= NW[4eVNiOjUEoHlCpC=> NULBeFZR\W6qYX7j[ZMhUVJvaX7keYNm\CCpZX7ldoF1cW:wIH;mJGRUSnN? MWWyOVQ6OjR6MB?=
PC3M-1E8 M1LzS2Z2dmO2aX;uJGF{e2G7 MoL5Nk42NzVxMUCg{txO NUHJS3BzOC12IHi= NHPVOYlqdmirYnn0d{B1cGViU2TBWFMh[WO2aY\heIlwdiCrbjDhJIRwe2VvIHHu[EB1cW2nLXTldIVv\GWwdDDtZY5v\XMEoB?= Mlj2NlUzPjF|NkW=
PC3M-1E8 MnvkSpVv[3Srb36gRZN{[Xl? MVuxNEDPxE1? MoniNlQhcA>? Ml\G[I94dnKnZ4XsZZRmeyCEY3ytfGwtKHO3co\peolvKGGwZDDjMW16[w>? NWrHTZVQOjV{NkGzOlU>
PC3M-1E8 NWfYdZBFTnWwY4Tpc44hSXO|YYm= NH;T[|AyOCEQvF2= NGnRTVIzPCCq Ml;WbY5pcWKrdIOgTWwuPiCrbnT1Z4VlKFOWQWSzJIFkfGm4YYTpc44h[W6mIITo[UBKVC14LXnu[JVk\WRiU2TBWFMh[WO2aY\heIlwdg>? NYTwR2Q2OjV{NkGzOlU>
PC3M-1E8 MWPDcI9vd2enbnnjJHN2en[rdnHsJGF{e2G7 NVzWRYxEOi53L{WvNVAh|ryP M{C1bIlvcGmkaYTzxsB1cGViY3;sc456KG[xcn3heIlwdiC|aXfubYZq[2GwdHz5 NE\yOVYzPTJ4MUO2OS=>
MDA-MB-231 M2HwV2Z2dmO2aX;uJGF{e2G7 M{OxcVIxKM7:TR?= MUOyJIg> NEi4TFZmgGirYnn0d{BUdmGrbDDhcoQhTS2lYXTo[ZJqdiCneIDy[ZN{cW:w MYiyOVE2OzN2OR?=
H9c2 NFPpRZJHfW6ldHnvckBCe3OjeR?= MlLKNlAhyrWP MWWzNEBucW5? MXfEUXNQyqB? M2P1[oFjd2yrc3jld{Bxem:yb3\vcE1qdmS3Y3XkJGFMXCCyaH;zdIhwenmuYYTpc44h[XRiYn;0bEB{\XJ2N{OgZY5lKHSqckOwPC=> Ml\TNlUyODVyNke=
HaCaT  NF;MWpVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoPkNVAhyrWP M2PkWVIxKG2rbh?= NHXBSWdFVVORwrC= Ml3P[Y5p[W6lZYOgd49z[W[nbnniMUBidmRic4XubZRqdmmkLXnu[JVk\WRiZ4Lve5RpKGmwaHnibZRqd25? Ml;kNlUxOTN7MEe=
Caki-1 Ml3RS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIDGT3cyOCEEtV2= NHfudmgzOCCvaX6= NVvWUYRKTE2VT9Mg Mk\a[Y5p[W6lZYOgd49z[W[nbnniMUBidmRic4XubZRqdmmkLXnu[JVk\WRiZ4Lve5RpKGmwaHnibZRqd25? NHP2eoMzPTBzM{mwOy=>
HaCaT  NUn1Z2d4SXCxcITvd4l{KEG|c3H5 MUexNEDDvU1? NFzzcXEzOCCvaX6= NWHuSGU6TE2VT9Mg MkTPbY5kemWjc3XzJJBzd3CxcoTpc45{KG:oIHHwc5B1d3SrYzDj[YxteyCmdXWgeI8hfHKnYYTt[Y51KHerdHigd49z[W[nbnniJI9zKHO3bnn0bY5q[g>? NX7mRpJvOjVyMUO5NFc>
FHL-primed hNSCs NF7CTm5E\WyuIG\pZYJqdGm2eTDBd5NigQ>? NXTKeHFbOC5yMj21JO69VQ>? M4\CNFczKGh? MVvs[YFleyC2bzD0bIUhdG:|czDv[kBk\WyuII\pZYJqdGm2eTDheEBpcWeqIHPvcoNmdnS{YYTpc44> M{\zd|I1QTR3NEO0
ELL-primed hNSCs NXm3NXlQS2WubDDWbYFjcWyrdImgRZN{[Xl? MXSwMlAzNTVizszN NHjWPGQ4OiCq NGTLTGNt\WGmczD0c{B1cGVibH;zd{Bw\iClZXzsJJZq[WKrbHn0fUBifCCqaXfoJINwdmOnboTyZZRqd25? MWOyOFk1PTR|NB?=
SS NY\rfmw2S2WubDDWbYFjcWyrdImgRZN{[Xl? NXLmXVRUOS1zMDFOwG0> MV:3NkBp NVnLUJRSTE2VTx?= MlPSZ4F2e2W|IHGg[I9{\S2mZYDlcoRmdnRiaX7obYJqfGmxbjDv[kB1cGVidnnhZoltcXS7wrC= MnexNlQ4PTZzMUG=
SeAx  NX;CNnk5S2WubDDWbYFjcWyrdImgRZN{[Xl? M4TVTVEuOTBizszN NYrMWIduPzJiaB?= NV3Sb3lqTE2VTx?= NYXrNoNM[2G3c3XzJIEh\G:|ZT3k[ZBmdmSnboSgbY5pcWKrdHnvckBw\iC2aHWgeoli[mmuaYT5xsA> MUOyOFc2PjFzMR?=
HuT-78 M1XzZ2NmdGxiVnnhZoltcXS7IFHzd4F6 Ml3HNU0yOCEQvF2= NWSxfZAyPzJiaB?= MUDEUXNQ M2TabYNifXOnczDhJIRwe2VvZHXw[Y5l\W62IHnubIljcXSrb36gc4YhfGinII\pZYJqdGm2edMg M{\WVVI1PzV4MUGx
CD4+ MmHORZBweHSxc3nzJGF{e2G7 MVKxNQKBkc7:bR?= MonmNlQhcA>? NWC4VpVETE2VTx?= MWDpcoR2[2W|IHHwc5B1d3OrczDzeJJwdmeueR?= NGe4NG0zPDd3NkGxNS=>
MCF-7 M4rVbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2S1XVAvPDZ7LUOuO|Uh|ryP NFHMZlg2KGR? NYHBNHNUemWmdXPld{Bk\WyuIH71cYJmeiC|aXfubYZq[2GwdHz5 MVeyOFczQDB5OB?=
MCF-7/LCC1 M4TTN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlvBNE41PjlvMz63OUDPxE1? MnPROUBl NX7Ue5A2emWmdXPld{Bk\WyuIH71cYJmeiC|aXfubYZq[2GwdHz5 MUOyOFczQDB5OB?=
MCF-7/LCC9 MljLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV\vPWRyOC52NkmtN{44PSEQvF2= NXfIbFdtPSCm NFTxNm5z\WS3Y3XzJINmdGxiboXtZoVzKHOrZ37p[olk[W62bIm= Ml7ENlQ4OjhyN{i=
HaCaT NIHpdGlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYKxNEDDvU1? MX2yNEBucW5? NIjFcYFFVVORwrC= MWrlcohidmOnczDleoVzd2yrbYXzMYlv\HWlZXSgZ4VtdCCpcn;3eIghcW6qaXLpeIlwdg>? MV6yOFQzOzF|MR?=
HaCaT NWS3UWdySXCxcITvd4l{KEG|c3H5 NGW2eocyOCEEtV2= NF7kdoozOCCvaX6= NYfJSGlmTE2VT9Mg MXzlcohidmOnczD0bIUh[XCxcITveIlkKGWoZnXjeJMhd2ZiZY\ldo9tcW23cx?= MVSyOFQzOzF|MR?=
MDA-MB-231 NUG3U5Q5TnWwY4Tpc44hSXO|YYm= M3r1U|ExKML3TR?= MoHoNlQhcA>? NGj6WoZFVVORwrC= M4XpNZJm\HWlZYOgVE1UXEGWMzDlfJBz\XO|aX;u M{DPUFI1Ozd4NUi2
SUM-159 NWLFVYFlTnWwY4Tpc44hSXO|YYm= NGnXb24yOCEEtV2= NFv1d5UzPCCq MXLEUXNQyqB? M3LHdJJm\HWlZYOgVE1UXEGWMzDlfJBz\XO|aX;u NIjrU40zPDN5NkW4Oi=>
SK-BR-3 MnfRSpVv[3Srb36gRZN{[Xl? MYexNEDDvU1? M3zrPVI1KGh? NWTJPJJ3TE2VT9Mg MULy[YR2[2W|IGCtV3RCXDNiZYjwdoV{e2mxbh?= NXvv[IFrOjR|N{[1PFY>
MCF7-HER2 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4PY[lAuOTBizszN NH\FS2Q1QCCq MV7EUXNQ M1\yOolv\HWlZYOgZ4VtdCCmZXH0bEBld3OnIHTldIVv\GWwdHz5 MUmyOFI6PzVyOB?=
MCF7-HER2 M1m2emZ2dmO2aX;uJGF{e2G7 NIH4cFE2KM7:TR?= NEn4N44zPCCq NYKwO2VJTE2VTx?= MmP3[IlucW6rc3jld{BUd3hvMjygU4N1NTRuIHHu[EB{dHWpIHX4dJJme3Orb36= NV\wd|d3OjR{OUe1NFg>
MCF7-HER2 NV;TfIszTnWwY4Tpc44hSXO|YYm= MnGxOUDPxE1? MXGyOEBp NHnUd4lFVVOR M1zLboRm[3KnYYPld{B1cGViZYjwdoV{e2mxbjDs[ZZmdHNib3[gSW1VKG2jcnvldpMtKH[rbXXueIlvKGGwZDDzcJVo NXnZVHJVOjR{OUe1NFg>
MCF7-HER2 NV;aeWplT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{HI[lUh|ryP MXmyOEBp NGf4S25FVVOR NV;PcYNR\W6qYX7j[ZMh[2WubDDndo94fGhiaX7obYJqfGmxbjDjc41jcW6nZDD3bZRpKEincnPldJRqdg>? NFP3XGUzPDJ7N{WwPC=>
HMECs  NH7sXm9HfW6ldHnvckBCe3OjeR?= NFHzcVMyOCEQvF2= NFLJV3MzKGh? M4ftN4lvcGmkaYTzJGlHVs7zwrDt[YRq[XSnZDDwbI9{eGixconsZZRqd25ib3[gV3RCXDFuIGPURXQzKGGwZDDTWGFVOw>? NIXlb3MzPDJzMUOyOy=>
HTR8/SVneo M4nIVWZ2dmO2aX;uJGF{e2G7 M1fZWlHDqM7:TdMg MWGxxsBp NI\Wb4R{fXCycnXzd4VlKE:VTT3pcoR2[2WmIGPURXQ{KHCqb4PwbI9zgWyjdHnvci=> NIDObHgzPDB4MEK0NS=>
HTR8/SVneo M{XCcWZ2dmO2aX;uJGF{e2G7 NIKyfYQxNjVxMdMg{txOyqB? MWO0PEBp M4LGfJJme3SxcnXzJJRp\SCneIDy[ZN{cW:wIH;mJGUu[2GmaHXybY4he3WycILld5Nm\CCkeTDPV20> NYPRTZBJOjRyNkCyOFE>
HTR8/SVneo M4fGVWZ2dmO2aX;uJGF{e2G7 M2XZRVHDqM7:TdMg MoLhOFghcA>? NIHJfXd{cWewaX\pZ4FvfGy7IHnuZ5Jm[XOnczDtbYdz[XSrb36gZpkhV1OPwrC= MVeyOFA3ODJ2MR?=
C13* NWDvfI5QSXCxcITvd4l{KEG|c3H5 MnK2NE0yOCEQvF2= MkHsNlQwPDhiaB?= NHfKfG9qdmS3Y3XzJIFxd3C2b4Ppd{BqdiCjIHTvd4Uh[W6mIITpcYUh\GWyZX7k[Y51KG2jbn7ldi=> M1rkeVI{QTZ{NUW4
OV2008 NX;wcGQ3SXCxcITvd4l{KEG|c3H5 NXXIR2VtOC1zMDFOwG0> NV7yZYVXOjRxNEigbC=> MnHMbY5lfWOnczDhdI9xfG:|aYOgbY4h[SCmb4PlJIFv\CC2aX3lJIRmeGWwZHXueEBu[W6wZYK= Mn\XNlM6PjJ3NUi=
C13* NGjybGdCeG:ydH;zbZMhSXO|YYm= Mn;iNlQwPDhiaB?= Moq1[Y5p[W6lZYOgZ4l{eGyjdHnuMYlv\HWlZXSgZZBweHSxc3nz NXfufmo3OjN7NkK1OVg>
OV2008 M2n1b2Fxd3C2b4Ppd{BCe3OjeR?= MmPONlQwPDhiaB?= NWLvVXlM\W6qYX7j[ZMh[2m|cHzheIlvNWmwZIXj[YQh[XCxcITvd4l{ M17aNlI{QTZ{NUW4
W480  M2LkXGZ2dmO2aX;uJGF{e2G7 NHLEPJIzNjVxMUCg{txO NFj1SnM{OCCvaX6= NV7PRnp5TE2VTx?= NHzw[Yl{\W6|aYTpfoV{KGOnbHzzJJRwKGOqZX3vdoFlcW:2aHXyZZB6KGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz NGnCXIEzOzl|NEm3Ni=>
SW837 MoLlSpVv[3Srb36gRZN{[Xl? M4XnSlIvPS9zMDFOwG0> NELVVZA{OCCvaX6= M{Tvd2ROW09? MnL5d4Vve2m2aYrld{Bk\WyuczD0c{BkcGWvb4Lh[IlwfGincnHwfUBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NYPrRllNOjN7M{S5O|I>
T24 M1;xemZ2dmO2aX;uJGF{e2G7 MnTTNk8yOC9{MDFOwG0> NYPPZnlqOjRiaB?= NX3vS|h5[2G3c3XzJIRwe2VvZHXw[Y5l\W62IHnubIljcXSrb36gc4YhfGinIFPYR2wyOi2rbnT1Z4VlKGmwY4LlZZNmKG:oIHnueoFlcW6pIHPlcIx{ NYrq[lI4OjN3Mk[wO|k>
CNE1 NGj5flNHfW6ldHnvckBCe3OjeR?= MnrUNlAhyrWP NYXMNnJQPDhiaB?= NUO2XmZX[myxY3vzJJRp\SCLTD22JIlv[3KnYYPl[EBxcG:|cHjvdplt[XSrb36gc4YhW3SjdEO= M3TMU|I{Ozh{OUG0
CNE2 MUjGeY5kfGmxbjDBd5NigQ>? NHn2[GYzOCEEtV2= Mn7YOFghcA>? MVjicI9kc3NidHjlJGlNNTZiaX7jdoVie2WmIIDoc5NxcG:{eXzheIlwdiCxZjDTeIF1Ow>? MViyN|M5OjlzNB?=
HONE1 MnzYSpVv[3Srb36gRZN{[Xl? NWXwe5VHOjBiwsXN NV;2eY9NPDhiaB?= MYricI9kc3NidHjlJGlNNTZiaX7jdoVie2WmIIDoc5NxcG:{eXzheIlwdiCxZjDTeIF1Ow>? MXOyN|M5OjlzNB?=
CNE1 NXzH[Zc3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1HZNlQh|ryP MVHzbYdvcW[rY3HueIx6KHKnZIXj[ZMh[2WubDD2bYFjcWyrdIm= M3j4[VI{Ozh{OUG0
CNE1 MnvpSpVv[3Srb36gRZN{[Xl? NWPxc|g3OC1{MDFOwG0> M4rFXFAuPCCq NFPXeVVqdmirYnn0d{BUfGG2MzDhZ5RqfmG2aX;uJIlvKGFiZH;z[U0h[W6mIITpcYUu\GWyZX7k[Y51KG2jbn7ldi=> NEHPdoMzOzN6MkmxOC=>
CNE2 MkD0SpVv[3Srb36gRZN{[Xl? NVzzdJJlOC1{MDFOwG0> MXOwMVQhcA>? NGPBcnFqdmirYnn0d{BUfGG2MzDhZ5RqfmG2aX;uJIlvKGFiZH;z[U0h[W6mIITpcYUu\GWyZX7k[Y51KG2jbn7ldi=> M1mxcFI{Ozh{OUG0
HONE1 NV3aT|VZTnWwY4Tpc44hSXO|YYm= MoL1NE0zOCEQvF2= M2n3NVAuPCCq NWnKdGRmcW6qaXLpeJMhW3SjdEOgZYN1cX[jdHnvckBqdiCjIHTvd4UuKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI> MYSyN|M5OjlzNB?=
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RAW264.7 NI\xfWFCeG:ydH;zbZMhSXO|YYm= MYK1M|ExKM7:TR?= M4\4b|Q2KG2rbh?= MX\lcohidmOnczD0c5hqdnNiaX7keYNm\CCjcH;weI9{cXNiYX7kJG1OWCCub4Pz MmP6NlI1PTR2M{G=
SW480 NFLxe4hE\WyuIG\pZYJqdGm2eTDBd5NigQ>? M2\0U|UwOTBxMkCg{txO MVe3NkBp M1rTe4lvcGmkaYTzJINmdGxidnnhZoltcXS7IH;mJJRp\SCDTFTIL{9ETDF|MzxCpINmdGy| MmDSNlE6ODB|OUe=
HCT116 NGrEVYVE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NH;6bFE2NzFyL{KwJO69VQ>? M{S1UFczKGh? MlrxbY5pcWKrdIOgZ4VtdCC4aXHibYxqfHlib3[geIhmKEGORFirM2NFOTN|K9MgZ4VtdHN? MkLFNlE6ODB|OUe=
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SNU387  M{fNSWNmdGxiVnnhZoltcXS7IFHzd4F6 NVT1VoEzOjEEoN88UeKh NXixUnJiOjRiaB?= M2DzfJJm\HWlZYOgZ4VtdCC4aXHibYxqfHl? MVGyNVMyOTl5NR?=
SNU398 NXXifmdTS2WubDDWbYFjcWyrdImgRZN{[Xl? MViyNOKh|ryPwrC= M3exNlI1KGh? M1PsN5Jm\HWlZYOgZ4VtdCC4aXHibYxqfHl? NV7K[4tqOjF|MUG5O|U>
HepG2 MmPoR4VtdCCYaXHibYxqfHliQYPzZZk> MYqyNOKh|ryPwrC= M{\wTlI1KGh? NXXDc4ZGemWmdXPld{Bk\WyuII\pZYJqdGm2eR?= M3fPVFIyOzFzOUe1
Huh-7 M1X5fGNmdGxiVnnhZoltcXS7IFHzd4F6 NF;F[YMzOMLizszNxsA> NF;iRlczPCCq MVzy[YR2[2W|IHPlcIwhfmmjYnnsbZR6 MUCyNVMyOTl5NR?=
VSMC MonvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF7WT|M{NzVxMUCg{txO MXWzNEBucW5? MmDUSG1UVw>? NHm3[m5xemW4ZX70d{BRTEeILTDhcoQhfGi{b33ibY4udWWmaXH0[YQhXlOPQzDwdo9tcW[ncnH0bY9vKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz NEPZV4MzODh2N{OwOi=>
MDA-MB-231 MYPBdI9xfG:|aYOgRZN{[Xl? MUGxNEDPxE1? MkHCNlQhcA>? M2\TbWROW00EoB?= NFPaUZdqdmS3Y3XzJIFxd3C2b4Ppdy=> M2OyeVE4OTF2MEC1
MDA-MB-435S NU\vOYZKSXCxcITvd4l{KEG|c3H5 M1G0VlExKM7:TR?= NUXyVmRuOjRiaB?= MWrEUXNQyqB? Mn[xbY5lfWOnczDhdI9xfG:|aYO= MknCNVcyOTRyMEW=

... Click to View More Cell Line Experimental Data

Protocol

Kinase Assay:

[1]

+ Expand

High-Throughput Screening and FluorescencePolarization Assays:

Screening is performed at approximately 30 °C. The specificity ofscreening hits is validated in analogous assays for binding of thetest compounds to the SH2 domains of STAT1, STAT5, and Lck. The final concentration of buffer components used for all FP assays is 10 mM HEPES (pH 7.5), 1 mM EDTA, 0.1% Nonidet P-40, 50 mM NaCl, and 10% DMSO. The absence of dithiothreitol is essential for inhibitory activity. The sequences of the peptides are: STAT3, 5-carboxyfluorescein-GY(PO3H2)LPQTV-NH2; STAT1, 5-carboxyfluorescein-GY(PO3H2)DKPHVL; STAT5, 5-carboxyfluorescein-GY(PO3H2)LVLDKW; and Lck, 5-carboxyfluorescein-GY(PO3H2)EEIP. For specificity analysis at 30 °C, proteins are used at 150 nM (STAT1, STAT3, and STAT5). For specificity analysis at 37 °C, proteins are used at 370 nM (STAT3) or 100 nM (Lck). Proteins are incubated with test compounds in Eppendorf tubes at the indicated temperatures for 60 min prior addition of therespective 5-carboxyfluorescein labeled peptides (final concentration: 10 nM).Before measurement at room temperature, the mixtures are allowed to equilibrate for at least 30 min. Test compounds are used at the indicated concentrations diluted from a 20× stock in DMSO. Binding curves and inhibition curves are fitted with SigmaPlot. All competition curves are repeated three times in independent experiment
Cell Research:

[2]

+ Expand
  • Cell lines: Ly3 cells
  • Concentrations: ~2.5 μM
  • Incubation Time: 48 h
  • Method:

    MTS


    (Only for Reference)
Animal Research:

[2]

+ Expand
  • Animal Models: Ly3 parental NOD/SCID IL2R null xenograft
  • Formulation: --
  • Dosages: 3.75 mg/kg every day
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 42 mg/mL (198.87 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order:
2% DMSO+30% PEG 300+H2O
For best results, use promptly after mixing.
8mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 211.19
Formula

C8H5NO4S

CAS No. 19983-44-9
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    We need to dissolve the compound for i.p. injection, could you give me some suggestions?

  • Answer:

    S7024 Stattic can be dissolved in 2% DMSO/PEG 300/dd H2O at 8 mg/mL as a clear solution.

STAT Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID