Stattic

Catalog No.S7024

Stattic Chemical Structure

Molecular Weight(MW): 211.19

Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1.

Size Price Stock Quantity  
In DMSO USD 90 In stock
USD 110 In stock
USD 170 In stock

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4 Customer Reviews

  • The cell viability was determined in HEC-1B cells treated with MPA, insulin, GSK1904529A or Stattic or with silenced DHCR24.

    Sci Rep, 2017. Stattic purchased from Selleck.

    LN229 cells were treated with vehicle or the small molecule STAT3 inhibitor Stattic and analyzed for cell migration (top panel) or cell invasion (bottom panel) by DAPI staining of nuclei. Bar graphs show quantification of cell motility.

    J Biol Chem 2013 288(8), 5553-61. Stattic purchased from Selleck.

  • (B) Western blotting showing the hepaCAM expression and the phosphorylation level of STAT3, ERK and AKT in cells treated with IL-6 (20 ng/mL) for 24 h with or without the pretreatment of three respective inhibitors, Stattic (10 μM), U0126 (5 μM) and MK-2206 (1 μM)

    Cell Signal, 2017, 32:48-58. Stattic purchased from Selleck.

    Western blot analysis of p-STAT3, total STAT3, ABCA1 and ABCG1 levels in cells pretreated with DMSO or Stattic (10 uM) for 1 hr before and during stimulation with rDKK1 in cells transfected with STAT3 siRNA or negative control, and rDKK1 added into the supernatant.

    FEBS Lett 2014 10.1016/j.febslet.2014.11.023. Stattic purchased from Selleck.

Purity & Quality Control

Choose Selective STAT Inhibitors

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Notes:

2. For more details, such as half maximal inhibitory concentrations (IC50s) and working concentrations of each inhibitor, please click on the link of the inhibitor of interest.
3. "+" indicates inhibitory effect. Increased inhibition is marked by a higher "+" designation.
4. Orange "√" refers to compounds which do inhibitory effects on the related isoform, but without specific value.

Biological Activity

Description Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1.
Features Stattic is the first non-peptide small molecule with inhibitory activity against STAT3 SH2 domain regardless of the STAT3 phosphorylation state in vitro.
Targets
STAT3 [1]
(Cell-free assay)
5.1 μM
In vitro

Stattic inhibits binding of a phosphotyrosine-containing peptide derived from the gp130 receptor to the STAT3 SH2 domain in a strongly temperature-dependent manner. Stattic only has a very weak effect on binding of a tyrosinephosphorylated peptide to the SH2-domain of the tyrosine kinase Lck. And it doesn’t inhibit dimerization of two other dimeric transcription factors (c-Myc/Max and Jun/Jun). It also inhibits fluorescein-labeled phosphopeptides to the SH2 domains of STAT1 and STAT5b. Stattic selectively inhibits DNA binding of STAT3 homodimers at a concentration of 10 μM. Shown to inhibit cellular phosphorylation of STAT3 at Tyr705 with little effect towards STAT1 phosphorylation at Tyr701 (in HepG2 cells) or the phosphorylation of JAK1, JAK2, and c-Src (in MDA-MB-231 and MDA-MB-235S cells). Stattic increases the apoptotic rate of STAT3-dependent breast cancer cell lines. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
H9c2 MX3GeY5kfGmxbjDBd5NigQ>? NIfFXWgyOOLCid88US=> MXW05qCKcA>? MojmdoV3\XK|ZYOgeIhmKGWoZnXjeJMhd2ZiSVytNlc> MYqyOlM{QTZ|Mx?=
NPC  NXPHblBlTnWwY4Tpc44hSXO|YYm= MVSwMVcvPSEEtV2= Mn:1ZYJwdGm|aHXzJGVOXC2uaXvlJI1wdGWldXzhdkBidHSncnH0bY9veyxiYX7kJINmdGxibXnndoF1cW:wIHHu[EBqdn[jc3nvckBqdmS3Y3XkJIJ6KFKNSWCgb45w[2umb4fu M1qyd|I2QTF3NEOw
HASMC NEnFcG1HfW6ldHnvckBCe3OjeR?= Mmn2NU4zPS13IN88US=> MVOyNEBucW5? Mn\5SG1UV8Li NFHiRVhqdmirYnn0d{BxNSi\KT3TWGFVNTFuMzy1JJNq\26jbIRCpC=> M3XYW|I2QDR7NkKy
H9c2  NUDw[40yTnWwY4Tpc44hSXO|YYm= NUf1R5dGOi9zMDFOwG0> NUXjWZRTOiCq MXnEUXNQyqB? NHfLbJNi[nKxZ3H0[ZPDqHSqZTDjfZRweHKxdHXjeIl3\SCnZn\lZ5R{KG:oIFnMMVI4KGGpYXnud5QhW0h? MnvVNlU5OjB7MEe=
A431 M4HaeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYCyxsDPxE1? MnfBNuKhcA>? Mo\WZoxw[2u|IFXHSk1z\X[ncoPl[EBl\WO{ZXHz[ZMhcW5iY3XscEB3cWGkaXzpeJk> MYqyOVczODR|NR?=
A431 NGj6VpBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHXQeZMzyqEQvF2= NGjpOY8zyqCq NY\1V2Z4cW6lcnXhd4V{KGmwIHHwc5B1d3OrczDpcoR2[2WmIHL5JJNpcWuxbnnu NF;Hb4szPTd{MESzOS=>
SiHa M2TuW2NmdGxiVnnhZoltcXS7IFHzd4F6 MmHsOU04PSCwTR?= MXiyOOKhcMLi MXjzbI94eyCvb4LwbI9td2e7IH;mJIEhfHmyaXPhcEBieG:ydH;0bYMh[2WubDDhcoQh\G:|ZT3k[ZBmdmSnboSgcI9{eyCxZjDj[YxtKH[rYXLpcIl1gcLi MoS4NlU2Ozl4NES=
SiHa NIexcXdHfW6ldHnvckBCe3OjeR?= MmrGOU04PSCwTR?= NVfQNVBiOjUEoHlCpC=> NGf2dZVz\WS3Y3XzJJRp\cLicHjvd5Bpd3K7bHH0bY9vKGG2IITo[UB1gXKxc3nu[UBz\XOrZIXlJFcxPcLi MV:yOVU{QTZ2NB?=
ECA109 NGHVZYpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmniNE0zOCEQvF2= NFTtSoczPMLiaNMg MlLMTWM2OD13LkWwJO69VQ>? MkHKNlU1QTJ2OEC=
TE13 NGnTR5lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV2wMVIxKM7:TR?= NWHYV2JjOjUEoHlCpC=> MVfJR|UxRTZwMUZCpO69VQ>? NETae3kzPTR7MkS4NC=>
KYSE150  MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoLZNE0zOCEQvF2= NV:4XpdbOjUEoHlCpC=> NEnmRYtKSzVyPUGyMlY1yqEQvF2= NH\R[Y4zPTR7MkS4NC=>
ECA109 MYHDcI9vd2enbnnjJHN2en[rdnHsJGF{e2G7 NEXoUlUxNjYEoN88US=> NVzwT|NDOjUEoHlCpC=> MXzzeZBxemW|c3XzJJRp\SClbH;uc4dmdmmlIH\vdo1ifGmxbh?= M1e5RlI2PDl{NEiw
TE13 NIPRUJlEdG:wb3flcolkKFO3co\peoFtKEG|c3H5 MXiwMlXDqM7:TR?= MnvwNlTDqGkEoB?= M1zEUJN2eHC{ZYPz[ZMhfGinIHPsc45w\2WwaXOg[o9zdWG2aX;u NYLLdFlvOjV2OUK0PFA>
KYSE150  NHyxUHFEdG:wb3flcolkKFO3co\peoFtKEG|c3H5 MkT5NE42yqEQvF2= NFXaWIUzPMLiaNMg NEjvVXl{fXCycnXzd4V{KHSqZTDjcI9vd2enbnnjJIZwem2jdHnvci=> NWfO[JZoOjV2OUK0PFA>
ECA109 NFrIdJNHfW6ldHnvckBCe3OjeR?= M{HJc|AvPcLizszN MV6yOOKhcMLi MY\lcohidmOnczDJVk1qdmS3Y3XkJIdmdmW{YYTpc44hd2ZiRGPCdy=> NH;veHkzPTR7MkS4NC=>
PC3M-1E8 M3zBNWZ2dmO2aX;uJGF{e2G7 M{DCflIvPS93L{GwJO69VQ>? MX:wMVQhcA>? NIHaSVZqdmirYnn0d{B1cGViU2TBWFMh[WO2aY\heIlwdiCrbjDhJIRwe2VvIHHu[EB1cW2nLXTldIVv\GWwdDDtZY5v\XMEoB?= NInXb3EzPTJ4MUO2OS=>
PC3M-1E8 M1TBNGZ2dmO2aX;uJGF{e2G7 MVKxNEDPxE1? Mlm0NlQhcA>? NET0eIZld3ewcnXneYxifGW|IFLjcE15VCxic4Xyeol3cW5iYX7kJIMuVXml M1uweFI2OjZzM{[1
PC3M-1E8 MkjJSpVv[3Srb36gRZN{[Xl? NIHheokyOCEQvF2= NUDIZZluOjRiaB?= M3PETIlvcGmkaYTzJGlNNTZiaX7keYNm\CCVVFHUN{Bi[3SrdnH0bY9vKGGwZDD0bIUhUUxvNj3pcoR2[2WmIGPURXQ{KGGldHn2ZZRqd25? M2izTFI2OjZzM{[1
PC3M-1E8 MkjrR4xwdm:pZX7pZ{BUfXK4aY\hcEBCe3OjeR?= MkjiNk42NzVxMUCg{txO M1\BO4lvcGmkaYTzxsB1cGViY3;sc456KG[xcn3heIlwdiC|aXfubYZq[2GwdHz5 MYiyOVI3OTN4NR?=
MDA-MB-231 MXvGeY5kfGmxbjDBd5NigQ>? MoTDNlAh|ryP MmnjNkBp M{PDRoV5cGmkaYTzJHNv[WmuIHHu[EBGNWOjZHjldolvKGW6cILld5Nqd25? MVGyOVE2OzN2OR?=
H9c2 NU\vT|NwTnWwY4Tpc44hSXO|YYm= NVK4TXRFOjBiwsXN M3P0VlMxKG2rbh?= Mmn1SG1UV8Li NYnEclR6[WKxbHnzbIV{KHC{b4Dv[o9tNWmwZIXj[YQhSUuWIIDoc5NxcG:{eXzheIlwdiCjdDDic5RpKHOnckS3N{BidmRidHjyN|A5 NH;4bpYzPTFyNUC2Oy=>
HaCaT  M{XzW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{i4d|ExKML3TR?= M{H4b|IxKG2rbh?= M{S5V2ROW00EoB?= NUPObmRi\W6qYX7j[ZMhe2:{YX\lcoljNSCjbnSgd5VvcXSrbnniMYlv\HWlZXSg[5Jwf3SqIHnubIljcXSrb36= Mn6wNlUxOTN7MEe=
Caki-1 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mo\xNVAhyrWP M3zlZVIxKG2rbh?= NG\ab2ZFVVORwrC= MnPV[Y5p[W6lZYOgd49z[W[nbnniMUBidmRic4XubZRqdmmkLXnu[JVk\WRiZ4Lve5RpKGmwaHnibZRqd25? MXeyOVAyOzlyNx?=
HaCaT  M4HTO2Fxd3C2b4Ppd{BCe3OjeR?= MmTNNVAhyrWP MUCyNEBucW5? MUnEUXNQyqB? MVPpcoNz\WG|ZYOgdJJweG:{dHnvcpMhd2ZiYYDvdJRwfGmlIHPlcIx{KGS3ZTD0c{B1emWjdH3lcpQhf2m2aDDzc5Ji\mWwaXKgc5Ihe3WwaYTpcolj MUWyOVAyOzlyNx?=
FHL-primed hNSCs M{fueWNmdGxiVnnhZoltcXS7IFHzd4F6 Mk\uNE4xOi13IN88US=> NWHRT|dGPzJiaB?= M1\Mfoxm[WS|IITvJJRp\SCub4PzJI9nKGOnbHygeoli[mmuaYT5JIF1KGirZ3igZ49v[2WwdILheIlwdg>? M3LXflI1QTR3NEO0
ELL-primed hNSCs MnTUR4VtdCCYaXHibYxqfHliQYPzZZk> NUHxdo5DOC5yMj21JO69VQ>? NG\W[GQ4OiCq NHLmNJlt\WGmczD0c{B1cGVibH;zd{Bw\iClZXzsJJZq[WKrbHn0fUBifCCqaXfoJINwdmOnboTyZZRqd25? NV\R[GJCOjR7NEW0N|Q>
SS MVfD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NEPJ[GIyNTFyIN88US=> NXHTOGRFPzJiaB?= MXHEUXNQ M4H6[4NifXOnczDhJIRwe2VvZHXw[Y5l\W62IHnubIljcXSrb36gc4YhfGinII\pZYJqdGm2edMg MoPtNlQ4PTZzMUG=
SeAx  MV;D[YxtKF[rYXLpcIl1gSCDc4PhfS=> MX:xMVExKM7:TR?= MWi3NkBp NFrFWllFVVOR NUjqNYhm[2G3c3XzJIEh\G:|ZT3k[ZBmdmSnboSgbY5pcWKrdHnvckBw\iC2aHWgeoli[mmuaYT5xsA> Mlu5NlQ4PTZzMUG=
HuT-78 MoXaR4VtdCCYaXHibYxqfHliQYPzZZk> NUDMbIRoOS1zMDFOwG0> M17q[VczKGh? M{DWPWROW09? NF[5fVZk[XW|ZYOgZUBld3OnLXTldIVv\GWwdDDpcohq[mm2aX;uJI9nKHSqZTD2bYFjcWyrdIpCpC=> NVqxe41lOjR5NU[xNVE>
CD4+ M{O3N2Fxd3C2b4Ppd{BCe3OjeR?= M1HJOlEx6oDLzszt NGm2OFIzPCCq MWTEUXNQ NYDrNY0{cW6mdXPld{BieG:ydH;zbZMhe3S{b37ncJk> NH7lNZkzPDd3NkGxNS=>
MCF-7 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVPufIZnOC52NkmtN{44PSEQvF2= NEjaTGg2KGR? NWrjS2d4emWmdXPld{Bk\WyuIH71cYJmeiC|aXfubYZq[2GwdHz5 NETRVVMzPDd{OEC3PC=>
MCF-7/LCC1 M4rqbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFfDWGMxNjR4OT2zMlc2KM7:TR?= NVe5PY5kPSCm NUf3TZl4emWmdXPld{Bk\WyuIH71cYJmeiC|aXfubYZq[2GwdHz5 MmnaNlQ4OjhyN{i=
MCF-7/LCC9 NHPTOVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2jGU|AvPDZ7LUOuO|Uh|ryP NH\yeVk2KGR? MlT3doVlfWOnczDj[YxtKG63bXLldkB{cWewaX\pZ4FvfGy7 NHzuSGIzPDd{OEC3PC=>
HaCaT M13NNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{\kblExKML3TR?= M4O2clIxKG2rbh?= M4DDSGROW00EoB?= NFf0UVZmdmijbnPld{BmfmW{b3zpcZV{NWmwZIXj[YQh[2WubDDndo94fGhiaX7obYJqfGmxbh?= Ml3iNlQ1OjNzM{G=
HaCaT MX7BdI9xfG:|aYOgRZN{[Xl? M3zqNlExKML3TR?= NYS4V4ttOjBibXnu NVPZO4RpTE2VT9Mg Monv[Y5p[W6lZYOgeIhmKGGyb4D0c5Rq[yCnZn\lZ5R{KG:oIHX2[ZJwdGmvdYO= NVr1[5A1OjR2MkOxN|E>
MDA-MB-231 M1zqZ2Z2dmO2aX;uJGF{e2G7 MnHvNVAhyrWP MWmyOEBp NHu5bZRFVVORwrC= NVPNPFN5emWmdXPld{BRNVOWQWSzJIV5eHKnc4Ppc44> M3vSNlI1Ozd4NUi2
SUM-159 MYHGeY5kfGmxbjDBd5NigQ>? M4HjXFExKML3TR?= MYKyOEBp MU\EUXNQyqB? NFvE[5lz\WS3Y3XzJHAuW1SDVEOg[ZhxemW|c3nvci=> Moq5NlQ{PzZ3OE[=
SK-BR-3 M{jzb2Z2dmO2aX;uJGF{e2G7 NV\Uc|RTOTBiwsXN M2j6eFI1KGh? NXfTOZRmTE2VT9Mg MUfy[YR2[2W|IGCtV3RCXDNiZYjwdoV{e2mxbh?= NX[4TYhkOjR|N{[1PFY>
MCF7-HER2 NH;XSlNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4XRXFAuOTBizszN NVrtbIpnPDhiaB?= MlPESG1UVw>? NX;wSGpHcW6mdXPld{Bk\WyuIHTlZZRpKGSxc3Wg[IVx\W6mZX70cJk> MWeyOFI6PzVyOB?=
MCF7-HER2 MVrGeY5kfGmxbjDBd5NigQ>? MUS1JO69VQ>? NH;zSHkzPCCq MULEUXNQ MVHkbY1qdmm|aHXzJHNwgC1{LDDPZ5QuPCxiYX7kJJNtfWdiZYjwdoV{e2mxbh?= MnHTNlQzQTd3MEi=
MCF7-HER2 NFXKNVNHfW6ldHnvckBCe3OjeR?= MYG1JO69VQ>? MUGyOEBp NHLDR5dFVVOR NIfycY9l\WO{ZXHz[ZMhfGinIHX4dJJme3Orb36gcIV3\Wy|IH;mJGVOXCCvYYLr[ZJ{NCC4aX3lcpRqdiCjbnSgd4x2\w>? M4\EdlI1Ojl5NUC4
MCF7-HER2 M3LzeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX32V4NOPSEQvF2= MXKyOEBp M3TaSWROW09? Mkm5[Y5p[W6lZYOgZ4VtdCCpcn;3eIghcW6qaXLpeIlwdiClb33ibY5m\CC5aYToJGhmemOncITpci=> MXGyOFI6PzVyOB?=
HMECs  M{fIOWZ2dmO2aX;uJGF{e2G7 MUGxNEDPxE1? M3XLdFIhcA>? NFTvdFlqdmirYnn0d{BKTk8QsdMgcYVlcWG2ZXSgdIhwe3Cqb4L5cIF1cW:wIH;mJHNVSVRzLDDTWGFVOiCjbnSgV3RCXDN? NUeyWJlmOjR{MUGzNlc>
HTR8/SVneo NWrtW3BnTnWwY4Tpc44hSXO|YYm= M{PBeFHDqM7:TdMg MXOxxsBp M1zCS5N2eHC{ZYPz[YQhV1OPLXnu[JVk\WRiU2TBWFMheGixc4Doc5J6dGG2aX;u NVf2PFFDOjRyNkCyOFE>
HTR8/SVneo NHrzb49HfW6ldHnvckBCe3OjeR?= MXWwMlUwOcLizszNxsA> NV\3Toc1PDhiaB?= NHHpZ25z\XO2b4Lld{B1cGViZYjwdoV{e2mxbjDv[kBGNWOjZHjldolvKHO3cIDy[ZN{\WRiYomgU3NO M1\BWlI1ODZyMkSx
HTR8/SVneo NGmzOXhHfW6ldHnvckBCe3OjeR?= MVexxsDPxE4EoB?= NI\YNW41QCCq M3njZZNq\26rZnnjZY51dHliaX7jdoVie2W|IH3p[5JifGmxbjDifUBQW04EoB?= MVmyOFA3ODJ2MR?=
C13* NX;wZlRlSXCxcITvd4l{KEG|c3H5 NFLkdmMxNTFyIN88US=> M2G4eVI1NzR6IHi= MojPbY5lfWOnczDhdI9xfG:|aYOgbY4h[SCmb4PlJIFv\CC2aX3lJIRmeGWwZHXueEBu[W6wZYK= MYCyN|k3OjV3OB?=
OV2008 NYXudnN{SXCxcITvd4l{KEG|c3H5 MXOwMVExKM7:TR?= NFLz[nozPC92ODDo NGnxSXZqdmS3Y3XzJIFxd3C2b4Ppd{BqdiCjIHTvd4Uh[W6mIITpcYUh\GWyZX7k[Y51KG2jbn7ldi=> M3\U[FI{QTZ{NUW4
C13* NEK4U3NCeG:ydH;zbZMhSXO|YYm= NGXkZW0zPC92ODDo NEnmNVFmdmijbnPld{BkcXOybHH0bY4ucW6mdXPl[EBieG:ydH;zbZM> NWm4fI5POjN7NkK1OVg>
OV2008 MlrSRZBweHSxc3nzJGF{e2G7 NXK2NpBHOjRxNEigbC=> MlXq[Y5p[W6lZYOgZ4l{eGyjdHnuMYlv\HWlZXSgZZBweHSxc3nz M{nBfVI{QTZ{NUW4
W480  NHXJ[2JHfW6ldHnvckBCe3OjeR?= MnvwNk42NzFyIN88US=> NYXxeFVnOzBibXnu MmHKSG1UVw>? M3TiWJNmdnOrdHn6[ZMh[2WubIOgeI8h[2inbX;yZYRqd3SqZYLhdJkhcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> M2jrblI{QTN2OUey
SW837 NWe0XGprTnWwY4Tpc44hSXO|YYm= NF[2NYEzNjVxMUCg{txO NHOwOYY{OCCvaX6= Mli5SG1UVw>? M2DxWJNmdnOrdHn6[ZMh[2WubIOgeI8h[2inbX;yZYRqd3SqZYLhdJkhcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> NWPrZZA6OjN7M{S5O|I>
T24 MUDGeY5kfGmxbjDBd5NigQ>? MlHTNk8yOC9{MDFOwG0> NGHOZpYzPCCq M1;Pe4NifXOnczDkc5NmNWSncHXu[IVvfCCrbnjpZol1cW:wIH;mJJRp\SCFWFPMNVIucW6mdXPl[EBqdmO{ZXHz[UBw\iCrbo\h[Ilv\yClZXzsdy=> NW\te2llOjN3Mk[wO|k>
CNE1 NFOzTnBHfW6ldHnvckBCe3OjeR?= NESwb44zOCEEtV2= MV:0PEBp MlXiZoxw[2u|IITo[UBKVC14IHnuZ5Jm[XOnZDDwbI9{eGixconsZZRqd25ib3[gV5RifDN? M1vGUVI{Ozh{OUG0
CNE2 NXvVT2JyTnWwY4Tpc44hSXO|YYm= NELFcIkzOCEEtV2= NYHrbVc{PDhiaB?= MmTPZoxw[2u|IITo[UBKVC14IHnuZ5Jm[XOnZDDwbI9{eGixconsZZRqd25ib3[gV5RifDN? M2rJTlI{Ozh{OUG0
HONE1 Mn7JSpVv[3Srb36gRZN{[Xl? NIPpZYUzOCEEtV2= MUW0PEBp NIHOSHRjdG:la4OgeIhmKEmOLU[gbY5kemWjc3XkJJBpd3OyaH;yfYxifGmxbjDv[kBUfGG2Mx?= NYPnVpc2OjN|OEK5NVQ>
CNE1 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXm0JO69VQ>? NYTN[W5Te2mpbnnmbYNidnSueTDy[YR2[2W|IHPlcIwhfmmjYnnsbZR6 NHHDOGczOzN6MkmxOC=>
CNE1 MoDtSpVv[3Srb36gRZN{[Xl? Mny2NE0zOCEQvF2= MoCwNE01KGh? MULpcohq[mm2czDTeIF1OyCjY4TpeoF1cW:wIHnuJIEh\G:|ZT2gZY5lKHSrbXWt[IVx\W6mZX70JI1idm6nch?= MoKyNlM{QDJ7MUS=
CNE2 M1LQUGZ2dmO2aX;uJGF{e2G7 NGfmfVgxNTJyIN88US=> M17Z[FAuPCCq NFm1WmhqdmirYnn0d{BUfGG2MzDhZ5RqfmG2aX;uJIlvKGFiZH;z[U0h[W6mIITpcYUu\GWyZX7k[Y51KG2jbn7ldi=> NUHUTJhDOjN|OEK5NVQ>
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PCNs M1O5[2NmdGxiVnnhZoltcXS7IFHzd4F6 MoXaNE0yOCEQvF2= MomwO|IhcA>? NYfMWphbTE2VTx?= MWfpcohq[mm2czDj[YxtKH[rYXLpcIl1gSCmb4PlJIRmeGWwZHXueIx6 M3SxbVI2PDN4Nkiy
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RAW264.7 NGL5ZZpHfW6ldHnvckBCe3OjeR?= NHHhc5gyOCEQvF2= NGL4[nAyOiCq NWD4cFNX[WK{b3fheIV{KHSqZTDtVm5CKGW6cILld5Nqd26|IH;mJGpCUzJuIGPURXQyNCCVVFHUNkwh[W6mIGPURXQ{KGmwZIXj[YQh[nliRF;OJIFv\CCWLUKgeI95cW5? MnK2NlI1PTR2M{G=
RAW264.7 MoHURZBweHSxc3nzJGF{e2G7 MX61M|ExKM7:TR?= NFfOS3A1PSCvaX6= MWrlcohidmOnczD0c5hqdnNiaX7keYNm\CCjcH;weI9{cXNiYX7kJG1OWCCub4Pz NV[2UHN4OjJ2NUS0N|E>
SW480 M3fLdGNmdGxiVnnhZoltcXS7IFHzd4F6 MVG1M|ExNzJyIN88US=> MWq3NkBp NYT5W|FXcW6qaXLpeJMh[2WubDD2bYFjcWyrdImgc4YhfGinIFHMSGgsN0OGMUOzL:Kh[2WubIO= MkfrNlE6ODB|OUe=
HCT116 MYXD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MVK1M|ExNzJyIN88US=> MmjuO|IhcA>? M2nsfYlvcGmkaYTzJINmdGxidnnhZoltcXS7IH;mJJRp\SCDTFTIL{9ETDF|MzxCpINmdGy| MmrmNlE6ODB|OUe=
DLD-1  MmfHR4VtdCCYaXHibYxqfHliQYPzZZk> Ml3FOU8yOC9{MDFOwG0> M2rFfFczKGh? M1HsSIlvcGmkaYTzJINmdGxidnnhZoltcXS7IH;mJJRp\SCDTFTIL{9ETDF|MzxCpINmdGy| M1r6[FIyQTByM{m3
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SNU398 M3S3eWNmdGxiVnnhZoltcXS7IFHzd4F6 M4\BOlIxyqEQvF5CpC=> NHPi[FgzPCCq MXHy[YR2[2W|IHPlcIwhfmmjYnnsbZR6 M1zUd|IyOzFzOUe1
HepG2 MojJR4VtdCCYaXHibYxqfHliQYPzZZk> M1fsVlIxyqEQvF5CpC=> MkOzNlQhcA>? MWfy[YR2[2W|IHPlcIwhfmmjYnnsbZR6 MVGyNVMyOTl5NR?=
Huh-7 M3XpNmNmdGxiVnnhZoltcXS7IFHzd4F6 MnjINlDDqM7:TdMg M1fSZlI1KGh? M3jvWZJm\HWlZYOgZ4VtdCC4aXHibYxqfHl? MU[yNVMyOTl5NR?=
VSMC M{fHcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXvLfZRyOy93L{GwJO69VQ>? NWjUe4hOOzBibXnu MXXEUXNQ MlqzdJJmfmWwdIOgVGRITi1iYX7kJJRpem:vYnnuMY1m\GmjdHXkJHZUVUNicILvcIln\XKjdHnvckBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> MU[yNFg1PzNyNh?=
MDA-MB-231 MVXBdI9xfG:|aYOgRZN{[Xl? NX\VNY5jOTBizszN NUmxVWppOjRiaB?= NHPubWhFVVORwrC= M{i1N4lv\HWlZYOgZZBweHSxc3nz MkTTNVcyOTRyMEW=
MDA-MB-435S M1TvcmFxd3C2b4Ppd{BCe3OjeR?= NHH4SGUyOCEQvF2= MVeyOEBp M2PBeGROW00EoB?= NXz3UGJScW6mdXPld{BieG:ydH;zbZM> NGW0[XUyPzFzNECwOS=>

... Click to View More Cell Line Experimental Data

Protocol

Kinase Assay:

[1]

+ Expand

High-Throughput Screening and FluorescencePolarization Assays:

Screening is performed at approximately 30 °C. The specificity ofscreening hits is validated in analogous assays for binding of thetest compounds to the SH2 domains of STAT1, STAT5, and Lck. The final concentration of buffer components used for all FP assays is 10 mM HEPES (pH 7.5), 1 mM EDTA, 0.1% Nonidet P-40, 50 mM NaCl, and 10% DMSO. The absence of dithiothreitol is essential for inhibitory activity. The sequences of the peptides are: STAT3, 5-carboxyfluorescein-GY(PO3H2)LPQTV-NH2; STAT1, 5-carboxyfluorescein-GY(PO3H2)DKPHVL; STAT5, 5-carboxyfluorescein-GY(PO3H2)LVLDKW; and Lck, 5-carboxyfluorescein-GY(PO3H2)EEIP. For specificity analysis at 30 °C, proteins are used at 150 nM (STAT1, STAT3, and STAT5). For specificity analysis at 37 °C, proteins are used at 370 nM (STAT3) or 100 nM (Lck). Proteins are incubated with test compounds in Eppendorf tubes at the indicated temperatures for 60 min prior addition of therespective 5-carboxyfluorescein labeled peptides (final concentration: 10 nM).Before measurement at room temperature, the mixtures are allowed to equilibrate for at least 30 min. Test compounds are used at the indicated concentrations diluted from a 20× stock in DMSO. Binding curves and inhibition curves are fitted with SigmaPlot. All competition curves are repeated three times in independent experiment
Cell Research:

[2]

+ Expand
  • Cell lines: Ly3 cells
  • Concentrations: ~2.5 μM
  • Incubation Time: 48 h
  • Method:

    MTS


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 42 mg/mL (198.87 mM)
Water <1 mg/mL
Ethanol <1 mg/mL
In vivo 2% DMSO+30% PEG 300+H2O 8mg/mL

* 1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 211.19
Formula

C8H5NO4S

CAS No. 19983-44-9
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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STAT Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID