Stattic

Catalog No.S7024

Stattic Chemical Structure

Molecular Weight(MW): 211.19

Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1.

Size Price Stock Quantity  
In DMSO USD 90 In stock
USD 110 In stock
USD 170 In stock
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4 Customer Reviews

  • The cell viability was determined in HEC-1B cells treated with MPA, insulin, GSK1904529A or Stattic or with silenced DHCR24.

    Sci Rep, 2017. Stattic purchased from Selleck.

    LN229 cells were treated with vehicle or the small molecule STAT3 inhibitor Stattic and analyzed for cell migration (top panel) or cell invasion (bottom panel) by DAPI staining of nuclei. Bar graphs show quantification of cell motility.

    J Biol Chem 2013 288(8), 5553-61. Stattic purchased from Selleck.

  • (B) Western blotting showing the hepaCAM expression and the phosphorylation level of STAT3, ERK and AKT in cells treated with IL-6 (20 ng/mL) for 24 h with or without the pretreatment of three respective inhibitors, Stattic (10 μM), U0126 (5 μM) and MK-2206 (1 μM)

    Cell Signal, 2017, 32:48-58. Stattic purchased from Selleck.

    Western blot analysis of p-STAT3, total STAT3, ABCA1 and ABCG1 levels in cells pretreated with DMSO or Stattic (10 uM) for 1 hr before and during stimulation with rDKK1 in cells transfected with STAT3 siRNA or negative control, and rDKK1 added into the supernatant.

    FEBS Lett 2014 10.1016/j.febslet.2014.11.023. Stattic purchased from Selleck.

Purity & Quality Control

Choose Selective STAT Inhibitors

Biological Activity

Description Stattic, the first nonpeptidic small molecule, potently inhibits STAT3 activation and nuclear translocation with IC50 of 5.1 μM in cell-free assays, highly selectivity over STAT1.
Features Stattic is the first non-peptide small molecule with inhibitory activity against STAT3 SH2 domain regardless of the STAT3 phosphorylation state in vitro.
Targets
STAT3 [1]
(Cell-free assay)
5.1 μM
In vitro

Stattic inhibits binding of a phosphotyrosine-containing peptide derived from the gp130 receptor to the STAT3 SH2 domain in a strongly temperature-dependent manner. Stattic only has a very weak effect on binding of a tyrosinephosphorylated peptide to the SH2-domain of the tyrosine kinase Lck. And it doesn’t inhibit dimerization of two other dimeric transcription factors (c-Myc/Max and Jun/Jun). It also inhibits fluorescein-labeled phosphopeptides to the SH2 domains of STAT1 and STAT5b. Stattic selectively inhibits DNA binding of STAT3 homodimers at a concentration of 10 μM. Shown to inhibit cellular phosphorylation of STAT3 at Tyr705 with little effect towards STAT1 phosphorylation at Tyr701 (in HepG2 cells) or the phosphorylation of JAK1, JAK2, and c-Src (in MDA-MB-231 and MDA-MB-235S cells). Stattic increases the apoptotic rate of STAT3-dependent breast cancer cell lines. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
H9c2 NFvhWY1HfW6ldHnvckBCe3OjeR?= MWmxNQKBkc7:TR?= M1ixNFTjiImq Mln2doV3\XK|ZYOgeIhmKGWoZnXjeJMhd2ZiSVytNlc> NFTVb|EzPjN|OU[zNy=>
NPC  NXvZPYp[TnWwY4Tpc44hSXO|YYm= NV24V|gzOC15LkWgxtVO MVnhZo9tcXOqZYOgSW1VNWyra3WgcY9t\WO3bHHyJIFtfGW{YYTpc45{NCCjbnSgZ4VtdCCvaXfyZZRqd25iYX7kJIlvfmG|aX;uJIlv\HWlZXSgZpkhWkuLUDDrco9kc2Sxd36= NEnxNHAzPTlzNUSzNC=>
HASMC M4nSW2Z2dmO2aX;uJGF{e2G7 NGezUHYyNjJ3LUWg{txO MmLVNlAhdWmw MUXEUXNQyqB? MmK4bY5pcWKrdIOgdE0pYSlvU2TBWE0yNDNuNTDzbYdv[Wy|wrC= MnLmNlU5PDl4MkK=
H9c2  M3HHcWZ2dmO2aX;uJGF{e2G7 NGPpfo0zNzFyIN88US=> NUnMb5psOiCq M36yPWROW00EoB?= Mn7SZYJzd2ejdHXzxsB1cGViY4n0c5Bzd3SnY4TpeoUh\W[oZXP0d{Bw\iCLTD2yO{Bi\2GrboP0JHNJ MlrINlU5OjB7MEe=
A431 NWDV[Wd1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWGyxsDPxE1? NXS0RWVrOsLiaB?= M1jtPYJtd2OtczDFS2YuemW4ZYLz[YQh\GWlcnXhd4V{KGmwIHPlcIwhfmmjYnnsbZR6 M2\x[VI2PzJyNEO1
A431 NUjjO3JrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mmj4NuKh|ryP M1:3ZlLDqGh? M1fLPYlv[3KnYYPld{BqdiCjcH;weI9{cXNiaX7keYNm\CCkeTDzbIlsd26rbh?= MXyyOVczODR|NR?=
SiHa MYTD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NInz[pQ2NTd3IH7N NYfBWGZxOjUEoHlCpC=> NEHtbIh{cG:5czDtc5JxcG:ub3f5JI9nKGFidInwbYNidCCjcH;weI91cWNiY3XscEBidmRiZH;z[U1l\XCnbnTlcpQhdG:|czDv[kBk\WyuII\pZYJqdGm2edMg MmfKNlU2Ozl4NES=
SiHa NITsZ25HfW6ldHnvckBCe3OjeR?= MkfjOU04PSCwTR?= NGnFSZYzPMLiaNMg NFz3c|Zz\WS3Y3XzJJRp\cLicHjvd5Bpd3K7bHH0bY9vKGG2IITo[UB1gXKxc3nu[UBz\XOrZIXlJFcxPcLi MU[yOVU{QTZ2NB?=
ECA109 NGLKOm9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVvlNmNjOC1{MDFOwG0> NI[4O5AzPMLiaNMg NEHpeFNKSzVyPUWuOVAh|ryP NFHnc3UzPTR7MkS4NC=>
TE13 NHn4O3hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWqwMVIxKM7:TR?= MYOyOOKhcMLi MV7JR|UxRTZwMUZCpO69VQ>? MmXQNlU1QTJ2OEC=
KYSE150  NXrOSnY2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWjO[GhoOC1{MDFOwG0> M4T6V|I1yqCqwrC= NVn3fol[UUN3ME2xNk43PMLizszN NUnnUGp6OjV2OUK0PFA>
ECA109 MUfDcI9vd2enbnnjJHN2en[rdnHsJGF{e2G7 MmXuNE42yqEQvF2= M{Xsc|I1yqCqwrC= NYO1XFd4e3WycILld5NmeyC2aHWgZ4xwdm:pZX7pZ{Bnd3KvYYTpc44> NUPFbW1[OjV2OUK0PFA>
TE13 MUfDcI9vd2enbnnjJHN2en[rdnHsJGF{e2G7 Mkj6NE42yqEQvF2= MkjzNlTDqGkEoB?= NVq3OGFHe3WycILld5NmeyC2aHWgZ4xwdm:pZX7pZ{Bnd3KvYYTpc44> M{XnR|I2PDl{NEiw
KYSE150  NXi5fmdqS2yxbn;n[Y5q[yCVdYL2bZZidCCDc4PhfS=> NUDOTWxpOC53wrFOwG0> MnK3NlTDqGkEoB?= MkTtd5VxeHKnc4Pld{B1cGViY3zvco9o\W6rYzDmc5Ju[XSrb36= NGn4ZnMzPTR7MkS4NC=>
ECA109 MofHSpVv[3Srb36gRZN{[Xl? M3jVeFAvPcLizszN MYOyOOKhcMLi Mnfz[Y5p[W6lZYOgTXIucW6mdXPl[EBo\W6ncnH0bY9vKG:oIFTTRpM> M3LNdVI2PDl{NEiw
PC3M-1E8 NYDGNIdmTnWwY4Tpc44hSXO|YYm= MVuyMlUwPS9zMDFOwG0> NGHjSXUxNTRiaB?= MXTpcohq[mm2czD0bIUhW1SDVEOgZYN1cX[jdHnvckBqdiCjIHTvd4UuKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZLDqA>? NIHLT|QzPTJ4MUO2OS=>
PC3M-1E8 MYfGeY5kfGmxbjDBd5NigQ>? MVmxNEDPxE1? MX[yOEBp NHLTb2Zld3ewcnXneYxifGW|IFLjcE15VCxic4Xyeol3cW5iYX7kJIMuVXml NV\ZOllZOjV{NkGzOlU>
PC3M-1E8 NIXKbGNHfW6ldHnvckBCe3OjeR?= MXqxNEDPxE1? NI\1dmwzPCCq Mn3QbY5pcWKrdIOgTWwuPiCrbnT1Z4VlKFOWQWSzJIFkfGm4YYTpc44h[W6mIITo[UBKVC14LXnu[JVk\WRiU2TBWFMh[WO2aY\heIlwdg>? MmX1NlUzPjF|NkW=
PC3M-1E8 NGrpUopEdG:wb3flcolkKFO3co\peoFtKEG|c3H5 M2TB[|IvPS93L{GwJO69VQ>? M{DreYlvcGmkaYTzxsB1cGViY3;sc456KG[xcn3heIlwdiC|aXfubYZq[2GwdHz5 MViyOVI3OTN4NR?=
MDA-MB-231 Mo\0SpVv[3Srb36gRZN{[Xl? M2LHSVIxKM7:TR?= MWCyJIg> NVj2TZRI\XiqaXLpeJMhW26jaXygZY5lKEVvY3HkbIVzcW5iZYjwdoV{e2mxbh?= Mn[xNlUyPTN|NEm=
H9c2 MofGSpVv[3Srb36gRZN{[Xl? MUiyNEDDvU1? MUmzNEBucW5? M4G1emROW00EoB?= MmHtZYJwdGm|aHXzJJBzd3CxZn;sMYlv\HWlZXSgRWtVKHCqb4PwbI9zgWyjdHnvckBifCCkb4ToJJNmejR5MzDhcoQhfGi{M{C4 NUPTRlZNOjVzMEWwOlc>
HaCaT  M2TpOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUDBSJBlOTBiwsXN MkfZNlAhdWmw M2fjeWROW00EoB?= MVXlcohidmOnczDzc5Ji\mWwaXKtJIFv\CC|dX7peIlvcWJvaX7keYNm\CCpcn;3eIghcW6qaXLpeIlwdg>? M4e3e|I2ODF|OUC3
Caki-1 M{S4TGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn;6NVAhyrWP NH;1SI0zOCCvaX6= NEjKbmpFVVORwrC= MX7lcohidmOnczDzc5Ji\mWwaXKtJIFv\CC|dX7peIlvcWJvaX7keYNm\CCpcn;3eIghcW6qaXLpeIlwdg>? M13IZlI2ODF|OUC3
HaCaT  NG\Hbo1CeG:ydH;zbZMhSXO|YYm= M3XPeFExKML3TR?= NUTYNGNkOjBibXnu M1fUWmROW00EoB?= NF7QXW1qdmO{ZXHz[ZMheHKxcH;yeIlwdnNib3[gZZBweHSxdHnjJINmdGy|IHT1[UB1dyC2cnXheI1mdnRid3n0bEB{d3KjZnXubYIhd3Jic4XubZRqdmmk NH;TZYQzPTBzM{mwOy=>
FHL-primed hNSCs NXHiNHh[S2WubDDWbYFjcWyrdImgRZN{[Xl? NGTMO3UxNjB{LUWg{txO Mlj6O|IhcA>? MlnDcIVi\HNidH:geIhmKGyxc4Ogc4Yh[2WubDD2bYFjcWyrdImgZZQhcGmpaDDjc45k\W62cnH0bY9v MWOyOFk1PTR|NB?=
ELL-primed hNSCs NWD5RpU5S2WubDDWbYFjcWyrdImgRZN{[Xl? M3vFNVAvODJvNTFOwG0> MlnpO|IhcA>? MnWzcIVi\HNidH:geIhmKGyxc4Ogc4Yh[2WubDD2bYFjcWyrdImgZZQhcGmpaDDjc45k\W62cnH0bY9v NF;t[3gzPDl2NUSzOC=>
SS MV3D[YxtKF[rYXLpcIl1gSCDc4PhfS=> MnvDNU0yOCEQvF2= M3TKVFczKGh? M4rkUGROW09? NX3ZXFli[2G3c3XzJIEh\G:|ZT3k[ZBmdmSnboSgbY5pcWKrdHnvckBw\iC2aHWgeoli[mmuaYT5xsA> NGjleYkzPDd3NkGxNS=>
SeAx  M3rofmNmdGxiVnnhZoltcXS7IFHzd4F6 MmnDNU0yOCEQvF2= MVK3NkBp NHexVI9FVVOR NIPh[Ydk[XW|ZYOgZUBld3OnLXTldIVv\GWwdDDpcohq[mm2aX;uJI9nKHSqZTD2bYFjcWyrdIpCpC=> NYK2VI5UOjR5NU[xNVE>
HuT-78 MUTD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MVixMVExKM7:TR?= MWm3NkBp MVXEUXNQ NGO2cYNk[XW|ZYOgZUBld3OnLXTldIVv\GWwdDDpcohq[mm2aX;uJI9nKHSqZTD2bYFjcWyrdIpCpC=> M4\yeFI1PzV4MUGx
CD4+ NWTIUlZnSXCxcITvd4l{KEG|c3H5 NF3nTZkyOOLCid88cS=> MXKyOEBp NIXoN2tFVVOR NWjNbFRPcW6mdXPld{BieG:ydH;zbZMhe3S{b37ncJk> M3jzfVI1PzV4MUGx
MCF-7 M1;m[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWGwMlQ3QS1|Lke1JO69VQ>? MXu1JIQ> MVny[YR2[2W|IHPlcIwhdnWvYnXyJJNq\26rZnnjZY51dHl? M2LNWFI1PzJ6MEe4
MCF-7/LCC1 NGPPWYZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUKwMlQ3QS1|Lke1JO69VQ>? NUXRTW9CPSCm MVLy[YR2[2W|IHPlcIwhdnWvYnXyJJNq\26rZnnjZY51dHl? NFHVTm0zPDd{OEC3PC=>
MCF-7/LCC9 NXPVflhKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3PGNVAvPDZ7LUOuO|Uh|ryP M4\IZ|Uh\A>? MV7y[YR2[2W|IHPlcIwhdnWvYnXyJJNq\26rZnnjZY51dHl? M{nVd|I1PzJ6MEe4
HaCaT NF7pcVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXTiWpExOTBiwsXN MYWyNEBucW5? NH;wRXlFVVORwrC= NGO3fVhmdmijbnPld{BmfmW{b3zpcZV{NWmwZIXj[YQh[2WubDDndo94fGhiaX7obYJqfGmxbh?= NIW4b5YzPDR{M{GzNS=>
HaCaT MVfBdI9xfG:|aYOgRZN{[Xl? MljtNVAhyrWP M1jjeVIxKG2rbh?= MkjYSG1UV8Li MXPlcohidmOnczD0bIUh[XCxcITveIlkKGWoZnXjeJMhd2ZiZY\ldo9tcW23cx?= Ml[wNlQ1OjNzM{G=
MDA-MB-231 MnX5SpVv[3Srb36gRZN{[Xl? MkXUNVAhyrWP MlnvNlQhcA>? MXrEUXNQyqB? NFHHdpdz\WS3Y3XzJHAuW1SDVEOg[ZhxemW|c3nvci=> MlP5NlQ{PzZ3OE[=
SUM-159 M{S1c2Z2dmO2aX;uJGF{e2G7 Mn7rNVAhyrWP Mnv2NlQhcA>? M132TmROW00EoB?= NUfmXId1emWmdXPld{BRNVOWQWSzJIV5eHKnc4Ppc44> MWmyOFM4PjV6Nh?=
SK-BR-3 MoizSpVv[3Srb36gRZN{[Xl? Ml\hNVAhyrWP MmLoNlQhcA>? MXnEUXNQyqB? M1KwXJJm\HWlZYOgVE1UXEGWMzDlfJBz\XO|aX;u NYX4e5pSOjR|N{[1PFY>
MCF7-HER2 MlTjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWGwMVExKM7:TR?= NH\ZcIM1QCCq MlLiSG1UVw>? Ml[3bY5lfWOnczDj[YxtKGSnYYToJIRwe2ViZHXw[Y5l\W62bIm= NH;tb5gzPDJ7N{WwPC=>
MCF7-HER2 NEfxSnRHfW6ldHnvckBCe3OjeR?= MkntOUDPxE1? MV2yOEBp M3\BXmROW09? M1rWWIRqdWmwaYPo[ZMhW2:6LUKsJG9kfC12LDDhcoQhe2y3ZzDlfJBz\XO|aX;u NWPoeGlGOjR{OUe1NFg>
MCF7-HER2 NGLud4JHfW6ldHnvckBCe3OjeR?= M{PP[FUh|ryP MVuyOEBp NH3LS4RFVVOR NFKzfZpl\WO{ZXHz[ZMhfGinIHX4dJJme3Orb36gcIV3\Wy|IH;mJGVOXCCvYYLr[ZJ{NCC4aX3lcpRqdiCjbnSgd4x2\w>? MV2yOFI6PzVyOB?=
MCF7-HER2 NFi1WVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF32NXc2KM7:TR?= MmG4NlQhcA>? NYHEWZNMTE2VTx?= MUHlcohidmOnczDj[YxtKGe{b4f0bEBqdmirYnn0bY9vKGOxbXLpcoVlKHerdHigTIVz[2WydHnu NVntZpdCOjR{OUe1NFg>
HMECs  MUTGeY5kfGmxbjDBd5NigQ>? NG[yWZgyOCEQvF2= NYHuSGt1OiCq MnrNbY5pcWKrdIOgTWZP|rIEoH3l[IlifGWmIIDoc5NxcG:{eXzheIlwdiCxZjDTWGFVOSxiU2TBWFIh[W6mIGPURXQ{ M2X4O|I1OjFzM{K3
HTR8/SVneo Ml;jSpVv[3Srb36gRZN{[Xl? NFP0UIsyyqEQvF5CpC=> NUHsSJNHOcLiaB?= MWTzeZBxemW|c3XkJG9UVS2rbnT1Z4VlKFOWQWSzJJBpd3OyaH;yfYxifGmxbh?= NG\KU3czPDB4MEK0NS=>
HTR8/SVneo MnK4SpVv[3Srb36gRZN{[Xl? NHfN[XQxNjVxMdMg{txOyqB? Ml;mOFghcA>? MlTIdoV{fG:{ZYOgeIhmKGW6cILld5Nqd25ib3[gSU1k[WSqZYLpckB{fXCycnXzd4VlKGK7IF;TUS=> MkHmNlQxPjB{NEG=
HTR8/SVneo MnrFSpVv[3Srb36gRZN{[Xl? MV6xxsDPxE4EoB?= MUm0PEBp MoW2d4lodmmoaXPhcpRtgSCrbnPy[YF{\XNibXnndoF1cW:wIHL5JG9UVcLi M13RR|I1ODZyMkSx
C13* NV;vVHVuSXCxcITvd4l{KEG|c3H5 MV2wMVExKM7:TR?= M{C5XVI1NzR6IHi= MWTpcoR2[2W|IHHwc5B1d3OrczDpckBiKGSxc3WgZY5lKHSrbXWg[IVx\W6mZX70JI1idm6nch?= NXyzPYcyOjN7NkK1OVg>
OV2008 M{fwWmFxd3C2b4Ppd{BCe3OjeR?= MnixNE0yOCEQvF2= MoDpNlQwPDhiaB?= MnTVbY5lfWOnczDhdI9xfG:|aYOgbY4h[SCmb4PlJIFv\CC2aX3lJIRmeGWwZHXueEBu[W6wZYK= MXGyN|k3OjV3OB?=
C13* M{[3V2Fxd3C2b4Ppd{BCe3OjeR?= MmnONlQwPDhiaB?= NHi4e3RmdmijbnPld{BkcXOybHH0bY4ucW6mdXPl[EBieG:ydH;zbZM> NUKzbnRYOjN7NkK1OVg>
OV2008 MYnBdI9xfG:|aYOgRZN{[Xl? MXiyOE81QCCq M1j1[oVvcGGwY3XzJINqe3CuYYTpck1qdmS3Y3XkJIFxd3C2b4Ppdy=> NVj4bHdyOjN7NkK1OVg>
W480  MV3GeY5kfGmxbjDBd5NigQ>? NIm5Z|IzNjVxMUCg{txO MnS3N|AhdWmw MXPEUXNQ MVXz[Y5{cXSrenXzJINmdGy|IITvJINp\W2xcnHkbY91cGW{YYD5JIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy M3:xNlI{QTN2OUey
SW837 MXTGeY5kfGmxbjDBd5NigQ>? M1vBXlIvPS9zMDFOwG0> M1n3clMxKG2rbh?= M1j0[WROW09? Ml:0d4Vve2m2aYrld{Bk\WyuczD0c{BkcGWvb4Lh[IlwfGincnHwfUBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NVvwTG9nOjN7M{S5O|I>
T24 MkDMSpVv[3Srb36gRZN{[Xl? NHvpeXgzNzFyL{KwJO69VQ>? MXGyOEBp M3;UOYNifXOnczDkc5NmNWSncHXu[IVvfCCrbnjpZol1cW:wIH;mJJRp\SCFWFPMNVIucW6mdXPl[EBqdmO{ZXHz[UBw\iCrbo\h[Ilv\yClZXzsdy=> MYKyN|UzPjB5OR?=
CNE1 NIq0U3ZHfW6ldHnvckBCe3OjeR?= M17PTFIxKML3TR?= M4XTblQ5KGh? M{K3XIJtd2OtczD0bIUhUUxvNjDpcoNz\WG|ZXSgdIhwe3Cqb4L5cIF1cW:wIH;mJHN1[XR| MkjpNlM{QDJ7MUS=
CNE2 MmHBSpVv[3Srb36gRZN{[Xl? NG\5V3kzOCEEtV2= NXryUIxOPDhiaB?= NYLGSGZW[myxY3vzJJRp\SCLTD22JIlv[3KnYYPl[EBxcG:|cHjvdplt[XSrb36gc4YhW3SjdEO= NFnobmszOzN6MkmxOC=>
HONE1 MoXwSpVv[3Srb36gRZN{[Xl? NHHRdmIzOCEEtV2= MX60PEBp NXj0fo5P[myxY3vzJJRp\SCLTD22JIlv[3KnYYPl[EBxcG:|cHjvdplt[XSrb36gc4YhW3SjdEO= NWjVN4NnOjN|OEK5NVQ>
CNE1 M1TQWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmjWOEDPxE1? M4jmPJNq\26rZnnjZY51dHlicnXkeYNmeyClZXzsJJZq[WKrbHn0fS=> MUOyN|M5OjlzNB?=
CNE1 MlLzSpVv[3Srb36gRZN{[Xl? NVn3VVRTOC1{MDFOwG0> MWiwMVQhcA>? MWnpcohq[mm2czDTeIF1OyCjY4TpeoF1cW:wIHnuJIEh\G:|ZT2gZY5lKHSrbXWt[IVx\W6mZX70JI1idm6nch?= M{f6XFI{Ozh{OUG0
CNE2 NXPuXXQ{TnWwY4Tpc44hSXO|YYm= MY[wMVIxKM7:TR?= MXuwMVQhcA>? M3nCZYlvcGmkaYTzJHN1[XR|IHHjeIl3[XSrb36gbY4h[SCmb4PlMUBidmRidHnt[U1l\XCnbnTlcpQhdWGwbnXy Mn7WNlM{QDJ7MUS=
HONE1 M3LyeWZ2dmO2aX;uJGF{e2G7 NIP6bGExNTJyIN88US=> M3LINVAuPCCq NWLLVGZ6cW6qaXLpeJMhW3SjdEOgZYN1cX[jdHnvckBqdiCjIHTvd4UuKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI> MXqyN|M5OjlzNB?=
CNE1 MYHD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MUewMlUuPjRizszN NIrCTmk1QCCq MXvzeZBxemW|c3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZT2gZY5lKHSrbXWt[IVx\W6mZX70JI1idm6nch?= M2XsWVI{Ozh{OUG0
CNE2 MkXoR4VtdCCYaXHibYxqfHliQYPzZZk> NUX0OGFjOC53LU[0JO69VQ>? NHuzT5o1QCCq Monnd5VxeHKnc4Pld{Bk\WyuII\pZYJqdGm2eTDpckBiKGSxc3WtJIFv\CC2aX3lMYRmeGWwZHXueEBu[W6wZYK= NFnCTm8zOzN6MkmxOC=>
HONE1 MYXD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NHrwdVkxNjVvNkSg{txO NWfpfJdoPDhiaB?= M{X1NZN2eHC{ZYPz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlMUBidmRidHnt[U1l\XCnbnTlcpQhdWGwbnXy MX6yN|M5OjlzNB?=
C666-1  M4nzR2NmdGxiVnnhZoltcXS7IFHzd4F6 NFT0THMxNjVvNkSg{txO M2XReFQ5KGh? NILROHJ{fXCycnXzd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[U0h[W6mIITpcYUu\GWyZX7k[Y51KG2jbn7ldi=> M3PkeFI{Ozh{OUG0
CNE1 NILMeoFCeG:ydH;zbZMhSXO|YYm= M1rGS|ExKML3TdMg NX7UTYpKPDhiaB?= M3PVN4lv\HWlZYOgZZBweHSxc3nzxsA> Mly1NlM{QDJ7MUS=
CNE2 MmPHRZBweHSxc3nzJGF{e2G7 MYOxNEDDvU4EoB?= NHrLfIg1QCCq Mmm1bY5lfWOnczDhdI9xfG:|aYRCpC=> NISyWZYzOzN6MkmxOC=>
HONE1 M1y0NmFxd3C2b4Ppd{BCe3OjeR?= Mlv0NVAhyrWPwrC= M3nwOFQ5KGh? MlnsbY5lfWOnczDhdI9xfG:|aYRCpC=> NF;iRmozOzN6MkmxOC=>
CNE2 NEGwTmhE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MUGxM|Ih|ryP NIi1epY1QCCq NHHPZop{\W6|aYTpfoUh[2WubIOgeI8hemGmaX;0bIVz[XC7 NXWxRZY5OjN|OEK5NVQ>
HONE1 NVTYZXA6S2WubDDWbYFjcWyrdImgRZN{[Xl? NIHlT4cyNzJizszN NUXKdYN7PDhiaB?= MnOyd4Vve2m2aYrlJINmdGy|IITvJJJi\GmxdHjldoFxgQ>? MXKyN|M5OjlzNB?=
C666-1  MlnER4VtdCCYaXHibYxqfHliQYPzZZk> MmLINU8zKM7:TR?= MVO0PEBp M4m4R5NmdnOrdHn6[UBk\WyuczD0c{Bz[WSrb4To[ZJieHl? M{f1c|I{Ozh{OUG0
HEC-1A MUDGeY5kfGmxbjDBd5NigQ>? M2nNOFHDqM7:TdMg M{\VVVI1KGh? M{LsfGROW09? M1GxdIJtd2OtczD0bIUhVVWFMkCt[Y5p[W6lZXSgbY53[XOrb36geJJq\2encnXkJIJ6KDFyJTDGRnM> NX3y[ZZFOjN{NkKyNFg>
RL95-2 M{O2bGZ2dmO2aX;uJGF{e2G7 MVWxxsDPxE4EoB?= M1y5NlI1KGh? MoPjSG1UVw>? NX\nOnV{[myxY3vzJJRp\SCPVVOyNE1mdmijbnPl[EBqdn[jc3nvckB1emmpZ3Xy[YQh[nliMUClJGZDWw>? M17VdVI{OjZ{MkC4
HEC-1A MnPrSpVv[3Srb36gRZN{[Xl? NInZOFgyyqEQvF5CpC=> MmTwNlQhcA>? M{TsUGROW09? M2DucoJtd2OtczD0bIUhVVWFMkCt[Y5p[W6lZXSgbY53[XOrb36geJJq\2encnXkJIJ6KEWJRtMg Mn;lNlMzPjJ{MEi=
RL95-2 M2\BPGZ2dmO2aX;uJGF{e2G7 MorhNeKh|ryPwrC= MkO2NlQhcA>? MkTkSG1UVw>? MoX6Zoxw[2u|IITo[UBOXUN{MD3lcohidmOnZDDpcpZie2mxbjD0dolo\2W{ZXSgZpkhTUeIwrC= MU[yN|I3OjJyOB?=
CT26 M4fY[2Z2dmO2aX;uJGF{e2G7 MmnWNlAhdU1? M4XoWVEhcA>? MoDld5VxeHKnc4Pld{BJT0ZvaX7keYNm\CCYRVfGJIV5eHKnc4Ppc47DqA>? M2HoflI{OjN|MU[z
UM-SCC-17B Mn7GS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXWwW5dHUUN3ME2yMlU3OiEEsTCwMlQxQSEQvF2sJGdKPTB;MT6yO|khyrFiMD6xPVQh|ryP NHPqdIozOjd5MEi5PS=>
OSC-19 Mkj1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWrJR|UxRTNwNEixJOKyKDBwOUWzJO69VSxiR1m1NF0yNjN4NjFCtUAxNjd5MDFOwG0> NGfpc4YzOjd5MEi5PS=>
Cal33 NFvoeVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYXJR|UxRTJwMkiyJOKyKDBwNEKzJO69VSxiR1m1NF0yNjN2OTFCtUAxNjN4MzFOwG0> NFj1O2QzOjd5MEi5PS=>
UM-SCC-22B M2HqbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4HqbGlEPTB;Mj62OFghyrFiMD61OFIh|ryPLDDHTVUxRTFwM{KwJOKyKDBwMkC0JO69VQ>? MU[yNlc4ODh7OR?=
UM-SCC-17B MoX5SpVv[3Srb36gRZN{[Xl? NVW0eIZYOC1|MDFOwG0> Ml\VNE0zPCCq NELZZZFqdmirYnn0d{BUXEGWMzDhZ5RqfmG2aX;uJIRwe2ViYX7kJJRqdWViZHXw[Y5l\W62bIm= Mly5NlI4PzB6OUm=
OSC-19 M4f2emZ2dmO2aX;uJGF{e2G7 NGX5Z4sxNTNyIN88US=> NGO2clAxNTJ2IHi= MnTTbY5pcWKrdIOgV3RCXDNiYXP0bZZifGmxbjDkc5NmKGGwZDD0bY1mKGSncHXu[IVvfGy7 NUjUT5hNOjJ5N{C4PVk>
Cal33 M2T5T2Z2dmO2aX;uJGF{e2G7 MmjQNE0{OCEQvF2= NXTqNmRLOC1{NDDo MWDpcohq[mm2czDTWGFVOyCjY4TpeoF1cW:wIHTvd4Uh[W6mIITpcYUh\GWyZX7k[Y51dHl? M1LaSlIzPzdyOEm5
UM-SCC-22B Mm\hSpVv[3Srb36gRZN{[Xl? M{TPNVAuOzBizszN NVTXdZY5OC1{NDDo MXfpcohq[mm2czDTWGFVOyCjY4TpeoF1cW:wIHTvd4Uh[W6mIITpcYUh\GWyZX7k[Y51dHl? MnK2NlI4PzB6OUm=
U-87MG NWfLenFlS2WubDDWbYFjcWyrdImgRZN{[Xl? MVSwMVExKM7:TR?= NH3KfZQ4OiCq MWDEUXNQ MnnnbY5pcWKrdIOgZ4VtdCC4aXHibYxqfHliZH;z[UBl\XCnbnTlcpRtgQ>? NWLMfpRbOjV2M{[2PFI>
U-373MG M1vyfGNmdGxiVnnhZoltcXS7IFHzd4F6 NGTSRVIxNTFyIN88US=> MnvJO|IhcA>? M2XNO2ROW09? MX3pcohq[mm2czDj[YxtKH[rYXLpcIl1gSCmb4PlJIRmeGWwZHXueIx6 MkPDNlU1OzZ4OEK=
SH-SY5Y MYDD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NUP3SIMxOC1zMDFOwG0> Mmn4O|IhcA>? MVvEUXNQ NX\5N3gycW6qaXLpeJMh[2WubDD2bYFjcWyrdImg[I9{\SCmZYDlcoRmdnSueR?= MnzRNlU1OzZ4OEK=
Tu-9648 NFfvSZlE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MlLaNE0yOCEQvF2= MnrLO|IhcA>? NUTrXXd5TE2VTx?= MXLpcohq[mm2czDj[YxtKH[rYXLpcIl1gSCmb4PlJIRmeGWwZHXueIx6 M4nqR|I2PDN4Nkiy
Neuro-2a NFXzSYhE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NEHHR4UxNTFyIN88US=> NFXBTnY4OiCq MV3EUXNQ NE\3dIJqdmirYnn0d{Bk\WyuII\pZYJqdGm2eTDkc5NmKGSncHXu[IVvfGy7 MXOyOVQ{PjZ6Mh?=
PCNs NGHOW4dE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NFiyfVIxNTFyIN88US=> MUi3NkBp MkfGSG1UVw>? MYjpcohq[mm2czDj[YxtKH[rYXLpcIl1gSCmb4PlJIRmeGWwZHXueIx6 MUiyOVQ{PjZ6Mh?=
PGCs MYjD[YxtKF[rYXLpcIl1gSCDc4PhfS=> M2jM[|AuOTBizszN MmD5O|IhcA>? MWPEUXNQ MW\pcohq[mm2czDj[YxtKH[rYXLpcIl1gSCmb4PlJIRmeGWwZHXueIx6 M32xVVI2PDN4Nkiy
RAW264.7 NWLHdZNKTnWwY4Tpc44hSXO|YYm= MmPFNVAh|ryP MUGxNkBp M4HNXoFjem:pYYTld{B1cGVibWLORUBmgHC{ZYPzbY9veyCxZjDKRWszNCCVVFHUNUwhW1SDVEKsJIFv\CCVVFHUN{BqdmS3Y3XkJIJ6KESRTjDhcoQhXC1{IITvfIlv NXPBTJpLOjJ2NUS0N|E>
RAW264.7 Ml31RZBweHSxc3nzJGF{e2G7 NXvXXZdJPS9zMDFOwG0> NXfHT4huPDVibXnu NUn4[mFS\W6qYX7j[ZMhfG:6aX7zJIlv\HWlZXSgZZBweHSxc3nzJIFv\CCPTWCgcI9{ew>? MWeyNlQ2PDR|MR?=
SW480 NVrnTWh4S2WubDDWbYFjcWyrdImgRZN{[Xl? MXu1M|ExNzJyIN88US=> NYixOXQ5PzJiaB?= NUnR[3JucW6qaXLpeJMh[2WubDD2bYFjcWyrdImgc4YhfGinIFHMSGgsN0OGMUOzL:Kh[2WubIO= NWnmTmloOjF7MECzPVc>
HCT116 M{e1Z2NmdGxiVnnhZoltcXS7IFHzd4F6 MYG1M|ExNzJyIN88US=> MmG3O|IhcA>? NYftTVJqcW6qaXLpeJMh[2WubDD2bYFjcWyrdImgc4YhfGinIFHMSGgsN0OGMUOzL:Kh[2WubIO= MYiyNVkxODN7Nx?=
DLD-1  NG\OOFdE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NYnITmpZPS9zMD:yNEDPxE1? NY\kcZBnPzJiaB?= MoXFbY5pcWKrdIOgZ4VtdCC4aXHibYxqfHlib3[geIhmKEGORFirM2NFOTN|K9MgZ4VtdHN? MmDQNlE6ODB|OUe=
SNU387  NGTvNXBE\WyuIG\pZYJqdGm2eTDBd5NigQ>? M{[4bFIxyqEQvF5CpC=> MojkNlQhcA>? M2TSN5Jm\HWlZYOgZ4VtdCC4aXHibYxqfHl? M2LITlIyOzFzOUe1
SNU398 M4HKbmNmdGxiVnnhZoltcXS7IFHzd4F6 MUCyNOKh|ryPwrC= M371Z|I1KGh? NFrpNXdz\WS3Y3XzJINmdGxidnnhZoltcXS7 NYLXRlBKOjF|MUG5O|U>
HepG2 MkPnR4VtdCCYaXHibYxqfHliQYPzZZk> MUmyNOKh|ryPwrC= M1zSVlI1KGh? MX\y[YR2[2W|IHPlcIwhfmmjYnnsbZR6 Ml3LNlE{OTF7N{W=
Huh-7 M3\xc2NmdGxiVnnhZoltcXS7IFHzd4F6 MoDSNlDDqM7:TdMg NFLqVHgzPCCq M1fsZpJm\HWlZYOgZ4VtdCC4aXHibYxqfHl? MmnVNlE{OTF7N{W=
VSMC MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGj4WFI{NzVxMUCg{txO MknCN|AhdWmw NF7tTZJFVVOR MnXMdJJmfmWwdIOgVGRITi1iYX7kJJRpem:vYnnuMY1m\GmjdHXkJHZUVUNicILvcIln\XKjdHnvckBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NUL4[G9lOjB6NEezNFY>
MDA-MB-231 M2r2fmFxd3C2b4Ppd{BCe3OjeR?= NFyw[I4yOCEQvF2= M4TSfVI1KGh? NGHZNVNFVVORwrC= M4TiVIlv\HWlZYOgZZBweHSxc3nz M1H6b|E4OTF2MEC1
MDA-MB-435S MlTrRZBweHSxc3nzJGF{e2G7 MYexNEDPxE1? M2XNVFI1KGh? Mn3YSG1UV8Li MY\pcoR2[2W|IHHwc5B1d3Orcx?= MnThNVcyOTRyMEW=

... Click to View More Cell Line Experimental Data

Protocol

Kinase Assay:

[1]

+ Expand

High-Throughput Screening and FluorescencePolarization Assays:

Screening is performed at approximately 30 °C. The specificity ofscreening hits is validated in analogous assays for binding of thetest compounds to the SH2 domains of STAT1, STAT5, and Lck. The final concentration of buffer components used for all FP assays is 10 mM HEPES (pH 7.5), 1 mM EDTA, 0.1% Nonidet P-40, 50 mM NaCl, and 10% DMSO. The absence of dithiothreitol is essential for inhibitory activity. The sequences of the peptides are: STAT3, 5-carboxyfluorescein-GY(PO3H2)LPQTV-NH2; STAT1, 5-carboxyfluorescein-GY(PO3H2)DKPHVL; STAT5, 5-carboxyfluorescein-GY(PO3H2)LVLDKW; and Lck, 5-carboxyfluorescein-GY(PO3H2)EEIP. For specificity analysis at 30 °C, proteins are used at 150 nM (STAT1, STAT3, and STAT5). For specificity analysis at 37 °C, proteins are used at 370 nM (STAT3) or 100 nM (Lck). Proteins are incubated with test compounds in Eppendorf tubes at the indicated temperatures for 60 min prior addition of therespective 5-carboxyfluorescein labeled peptides (final concentration: 10 nM).Before measurement at room temperature, the mixtures are allowed to equilibrate for at least 30 min. Test compounds are used at the indicated concentrations diluted from a 20× stock in DMSO. Binding curves and inhibition curves are fitted with SigmaPlot. All competition curves are repeated three times in independent experiment
Cell Research:

[2]

+ Expand
  • Cell lines: Ly3 cells
  • Concentrations: ~2.5 μM
  • Incubation Time: 48 h
  • Method:

    MTS


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 42 mg/mL (198.87 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents individually and in order:
2% DMSO+30% PEG 300+H2O
8mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 211.19
Formula

C8H5NO4S

CAS No. 19983-44-9
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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STAT Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID