Home JAK/STAT STAT inhibitor Narciclasine For research use only.

Narciclasine

Synonyms: Lycoricidinol

Narciclasine (Lycoricidinol), an isocarbostyril alkaloid narciclasine, targets STAT3 via distinct mechanisms in tamoxifen-resistant breast cancer cells. Narciclasine suppresses phosphorylation, activation, dimerization, and nuclear translocation of STAT3 by directly binding with the STAT3 SH2 domain and leads STAT3 degradation. Narciclasine is a plant growth modulator. Narciclasine modulates the Rho/Rho kinase/LIM kinase/cofilin signaling pathway, greatly increasing GTPase RhoA activity as well as inducing actin stress fiber formation in a RhoA-dependent manner.

Narciclasine Chemical Structure

Narciclasine Chemical Structure

CAS: 29477-83-6

Selleck's Narciclasine has been cited by 2 publications

Purity & Quality Control

Batch: E112401 DMSO] 61 mg/mL] false] ] ] false] ] ] false Purity: 99.74%
99.74

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Signaling Pathway

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Biological Activity

Description Narciclasine (Lycoricidinol), an isocarbostyril alkaloid narciclasine, targets STAT3 via distinct mechanisms in tamoxifen-resistant breast cancer cells. Narciclasine suppresses phosphorylation, activation, dimerization, and nuclear translocation of STAT3 by directly binding with the STAT3 SH2 domain and leads STAT3 degradation. Narciclasine is a plant growth modulator. Narciclasine modulates the Rho/Rho kinase/LIM kinase/cofilin signaling pathway, greatly increasing GTPase RhoA activity as well as inducing actin stress fiber formation in a RhoA-dependent manner.
Targets
STAT3 [1]
In vitro
In vitro

Narciclasine activates Rho and stress fibers in glioblastoma multiforme cells. The mean IC50 of ~50 nM calculated on the 6 human glioblastoma multiforme (U373, Hs683, GL19, GL5, GL16, GL17), The mean IC50 value of 47 nM for Narciclasine across a panel of 60 cancer cell lines. [2]
Cell Research Cell lines Human glioblastoma multiforme cell lines
Concentrations 0.001-10 μM
Incubation Time 72 h
Method

The cells are incubated for 72 h in the presence and absence of the narciclasine (0.001-10 μM) for the determination of narciclasine IC50 values by colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.
In Vivo
In vivo

Narciclasine significantly increases the survival of GL19 glioblastoma multiforme-bearing mice, and the survival of Hs683 glioblastoma multiforme-bearing mice. [2]
Animal Research Animal Models GL19 glioblastoma multiforme-bearing mice
Dosages 1 mg/kg
Administration i.v., p.o.

Chemical Information & Solubility

Molecular Weight 307.26 Formula

C14H13NO7
CAS No. 29477-83-6 SDF --
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 61 mg/mL ( (198.52 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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