Molecular Weight(MW): 422.46
HS-173 is a potent PI3Kα inhibitor with IC50 of 0.8 nM.
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b, d Serum-deprived MG-63 (b) and U20S cells (d) were pre-treated with 1 nmol/L HS-173 (PI3Kα inhibitor), 10 nmol/L TGX-221 (PI3Kβ inhibitor), 10 nmol/L CZC24832 (PI3Kγ inhibitor) and 10 nmol/L CAL-101 (PI3Kδ inhibitor) for 1 h, then were incubated with 100 ng/mL Wnt5a and harvested at 30 min after the start of Wnt5a treatment. Data were presented as mean ± SD of 3 determinations. The relative RhoA activity was normalized to the average value of each inhibitor-untreated group
Cancer Cell Int, 2017, 17:27. HS-173 purchased from Selleck.
Purity & Quality Control
Choose Selective PI3K Inhibitors
|Description||HS-173 is a potent PI3Kα inhibitor with IC50 of 0.8 nM.|
HS-173 shows potent antiproliferative effects in T47D, SK-BR3, and MCF7 cells with IC50 of 0.6, 1.5, and 7.8 μM, respectively.  HS-173 causes complete suppression of the PI3K pathway in cancer cell lines (Hep3B and SkBr3). Moreover, HS-173 induces cell apoptosis by affecting cell-cycle distribution and activating caspases, and block VEGF-induced angiogenesis in vitro.  Combined treatment with Sorafenib and HS-173 has a synergistic anti-cancer effect on pancreatic cancer cells. 
|In vivo||HS-173 diminishes blood vessel formation in mice.  HS-173 markedly attenuates the development of liver fibrosis by blocking PI3K/Akt signaling in vivo. |
PI3-Kinase assay:The PI3K assay is performed using the Kinase-Glo Max luminescent kinase assay kit which quantifies the amount of ADP produced by the PI3K reaction. In brief, an active PI3K (100 ng) is preincubated with compound for 5 min in kinase reaction buffer (25 mM MOPS [pH 7.0], 5 mM MgCl2, and 1 mM EGTA) and 10 μg l-α-phosphatidylinositol (PI). Before addition of PI, it is sonicated with sonication buffer (25 mM MOPS [pH 7.0], 1 mM EGTA) in water for 20 min for allowing miscelle formation. Then reaction is started by the addition of 10 μM ATP and ran for 180 min. To terminate kinase reaction, same volume of Kinase-Glo Max buffer is added. After 10 min, the plates are then read on a GloMax plate reader for luminescence detection.
|In vitro||DMSO||84 mg/mL warmed (198.83 mM)|
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
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This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )
* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).
Molecular Weight Calculator
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Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
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