Doxorubicin (Adriamycin) HCl

Licensed by Pfizer Catalog No.S1208 Synonyms: NSC 123127

Doxorubicin (Adriamycin) HCl Chemical Structure

Molecular Weight(MW): 579.98

Doxorubicin (Adriamycin) HCl is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage and apoptosis in tumor cells.

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Cited by 32 Publications

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  • Enhancement of apoptotic response by addition of targeted agents lapatinib and sunitinib to doxorubicin in the same device reservoir at 24 hours. P values were determined using Student’s t test for all graphs.

    Sci Transl Med, 2015, 7(284): 284ra57 . Doxorubicin (Adriamycin) HCl purchased from Selleck.

    Cell viabilities with increasing concentrations of cisplatin (CP) and doxorubicin (DOXO) under normoxic and hypoxic condition for 48 hours were determined by MTT assay. IC50 values are presented as the means ?SDs (n=4) and * denotes p<0.05.

    Cancer Res 2014 74, 298-308. Doxorubicin (Adriamycin) HCl purchased from Selleck.

  • Cleavage of caspase-3, -9 and the expression of PARP were measured by western blot using b-actin as a loading control.

    Cell Cycle, 2017.. Doxorubicin (Adriamycin) HCl purchased from Selleck.

    (C) & (D) Treatments with doxorubicin, cisplatin or AZD6244 further decreased the cell viability in RH30 cloned cells in which STAT3 or GP130 was knockdown by shRNA. (*P<.05, ** P <.01, *** P <.001).

    Curr Cancer Drug Targets, 2016, 16(7):631-8. Doxorubicin (Adriamycin) HCl purchased from Selleck.

  •  

    Doxorubicin release of P(MAA-g-EG) and P(MAA-g-EG) containing nanoparticles in two-step pH conditions. Doxorubicinloaded P(MAA-g-EG) ,P(MAA-g-EG)-1.0NP ,P(MAA-g-EG)-2.5NP, and P(MAA-g-EG)-5.0NP crushed particles (75–150 um) were released in 1 PBS (pH 2.0) for 90 min. Then the pH was increased to 7.0 by adding 5N NaOH and release continued for 6 h.

    J Biomed Mater Res Part A 2012 10.1002/jbm.a.34064. Doxorubicin (Adriamycin) HCl purchased from Selleck.

    Temporal uptake of free DOX or DOX-PCL63-b-PNVP90 micelles into K-562 (I) and DL (J) cells were studied by incubating in 24-well plates at a concentration of 50,000 cells per well. Representative images shown were obtained using a fluorescence microscope Eclipse 80i (Nikon, Japan) (Plan Fluor, 40X, NA 0.75 objective) equipped with blue and red filters for, Hoechst and Doxorubicin respectively (n = 3).

    PLoS One 2014 9, e94309. Doxorubicin (Adriamycin) HCl purchased from Selleck.

  • PLoS One 2013 8(1), e54595. Doxorubicin (Adriamycin) HCl purchased from Selleck.

    CON+ -positive control (with FBS as chemoattractant); CON- - negative control (without chemoattractant); DOX -doxorubicin; WZ -WZ811; PF- PF-573228; * -significant difference compared to positive control: p<0.05; ** -significant difference compared to positive control: p<0.01; # -significant difference between combination and inhibitor only: p<0.05. Cancer cell invasion was analyzed using Transwell membranes assembled in 24-well plates and covered with a layer of Matrigel. COR-L23 cells were treated for 24 h with 50 nM DOX, 10 uM WZ811, 5 uM PF-573228 and combinations of inhibitors with 50 nM DOX. Similarly, RH460 cells were treated for 24 h with 1 uM DOX, 10 uM WZ811, 2.5 uM PF-573228 and combinations of inhibitors with 1 uM DOX. Applied doses were the ones that exerted the most pronounced synergistic effect on cytotoxicity level. After the incubation period, cells that invaded through matrigel and membranes were fixed in 4% paraformaldehyde-PBS and stained with Hoechst 33342 nuclear stain. Cells were visualized and counted under a fluorescent microscope at 10x magnification.

    2014 Dr Milica Pesic from Institute for Biological Research. Doxorubicin (Adriamycin) HCl purchased from Selleck.

Purity & Quality Control

Choose Selective Topoisomerase Inhibitors

Biological Activity

Description Doxorubicin (Adriamycin) HCl is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage and apoptosis in tumor cells.
Targets
Topo II [1]
(Tumor cells)
In vitro

Doxorubicin, an antibiotic anthracycline, is commonly considered to exert its anti-tumor activity at two fundamental levels, altering DNA and producing free radicals to trigger apoptosis of cancer cells through DNA damage. Doxorubicin can block the synthesis of DNA by intercalating into the DNA strand, and inhibits DNA topoisomerase II (TOP2). Doxorubicin is most effective when cells are rapidly proliferating and expressing high levels of TOP2. Additionally, Doxorubicin can trigger apoptosis by producing ceramide (which prompts apoptosis by activating p53 or other downstream pathways such as JNK), the degradation of Akt by serine threonine proteases, the mitochondrial release of cytochrome c, increased FasL (death receptor Fas/CD95 ligand) mRNA production, and a greater production of free radicals. [2] Pre-treatment with GSNO (nitrosoglutathione) suppresses the resistance in the doxorubicin-resistant breast cancer cell line MCF7/Dx, accompanied by enhanced protein glutathionylation and accumulation of doxorubicin in the nucleus. [3] Doxorubicin induced G2/M checkpoint arrest are attributed to elevated cyclin G2 (CycG2) expression and phospho-modification of proteins in the ataxia telangiectasia mutated (ATM) and ATM and Rad3-related (ATR) signaling pathways. [5] Doxorubicin inhibits AMP-activated protein kinase (AMPK), resulting in SIRT1 dysfunction, p53 accumulation, and increased cell death in mouse embryonic fibroblasts (MEFs) and cardiomyocytes, which can be further sensitized by pre-inhibition of AMPK. [6] Doxorubicin elicits a marked heat shock response, and that either inhibition or silencing of heat shock proteins enhance the Doxorubicin apoptotic effect in neuroblastoma cells. Nanomolar Doxorubicin treatment of neuroblastoma cells causes dose-dependent over-ubiquitination of a specific set of proteins in the absence of measurable inhibition of proteasome, and loss of activity of ubiquitinated enzymes such as lactate dehydrogenase and α-enolase, the protein ubiquination patterns of which is similar to those with proteasome inhibitor Bortezomib, indicating that Doxorubicin may also exert its effect by damaging proteins. [8]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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Yoshida sarcoma MlHlSpVv[3Srb36gRZN{[Xl? MnToTY5pcWKrdHnvckBw\iCdM1jdWIRTKGmwY3;ydI9z[XSrb36ge4l1cCCLQ{WwJI9nKDBwMEKg{txO NXXpbm5QOTN{Mkm4Oi=>
CHO MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX;iXINrUUN3ME2wMlA3KM7:TR?= Mn;RNVM2PDd3MB?=
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8226/ADR NWTsZ5RtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3myZmlEPTB;MD61OkDPxE1? MnTrNVQ1Pzd|MB?=
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H69 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXjJR|UxRTBwOEeg{txO MUSxOFc6PTh3
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H9 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1nFPGlvcGmkaYTpc44hd2ZiSEmgZ4VtdHNiY3jyc45q[2GubImgbY5n\WO2ZXSge4l1cCC2aHWgTGlXNTFiKFi5M2lKUUJrIIfpeIghUUR3MDDv[kAxNjBzIN88US=> NYWzSYpoOTV2OE[4NS=>
T-C8166 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGWydZpKTDVyPUCuNFEh|ryP NF[2UHEyPTR6Nkix
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T-MT4 NInKSXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWe0PYpyUUR3ME2wMlAyKM7:TR?= MlW1NVU1QDZ6MR?=
SKMEL-5 M{W5Vmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFO2[WZGTDVyPUOuNkDPxE1? MVexOlE{PzV|
MLM NWjwV2hQS3m2b4TvfIlkKEG|c3H5 MULFSFUxRTBwMUOg{txO NYeyTphPOTh5NUO1NC=>
KBM-3 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWHJOHVWUUN3ME2wMlAzPiEQvF2= NU\VV2tmOTl7NUi3Oy=>
HT-29 M1;IZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2T3[|AvODFizszn MoX0OFghcA>? NVi1bXVQSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJk> NWXDO4FROjd5OES0PS=>
EMT6 Mk\kS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmPWNE42KM7:TR?= NIDDcVEyKGh? NIjxZWpKdmirYnn0bY9vKG:oIIDyc4xq\mW{YYTpc44h[nlibXXhd5VzcW6pIH\vdkB{fXK4aY\pcoch\nKjY4Tpc45{KHerdHigSWQ2OCCxZjCwMlAxODFizszN M{Xhe|c1PTJ4N{S=
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C38 MoKwR5l1d3SxeHnjJGF{e2G7 NH\1b3pKSzVyPUCuNFEh|ryP MlrJPFA2PzJ7MR?=
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MCF Ml3HSpVv[3Srb36gRZN{[Xl? NIjYe2pT\X[ncoPhcEBw\iCSLXfwMY1m\GmjdHXkJI12dHSrZIL1[{Bz\XOrc4ThcoNmKGG|c3Xzd4VlKGG|IHXubIFv[2WvZX70JIlvKGO7dH;0c5hq[2m2eTDv[kBi\HKrYX35Z4lv NV6ze2JlQDl6NEG1NS=>
C6 M1K3TWN6fG:2b4jpZ{BCe3OjeR?= M3O4NVIvPSCvZz;tUC=> MkLyO|IhcA>? MXHEUXNQ Mo\RR5l1d3SxeHnjbZR6KGGpYXnud5QhemG2IFO2JINmdGy|IHH0JFUxKHWpL33MJJRwKDJwNTDt[{9uVA>? M{T0ZVg6QDh4MES=
C6 M2q5cGZ2dmO2aX;uJGF{e2G7 NWL4T3Q3Oi53IH3nM41N NUHwXWFSPCCq NIPJSWFFVVOR M{n1VGlvcGmkaYTpc44hd2ZidIXieYxqdiCyb3z5cYVzcXqjdHnvci=> M3\1clg6QDh4MES=
C6 MV7DfZRwfG:6aXOgRZN{[Xl? NGrrb|EzNjVibXevcWw> M3G5O|UhcA>? MULEUXNQ NYHBN49iS3m2b4TvfIlkcXS7IHHzd4V{e2WmIHHzJINmdGxicnXs[YF{\Q>? MlTRPFk5QDZyNB?=
SKVLB NUOzNGdHS3m2b4TvfIlkKEG|c3H5 MnzDO|IhcA>? NVnmc3VqUUN3ME22MlU1KM7:TR?= M4j3eVkxODN3MkC=
MCF7/VP16 M2XvfmN6fG:2b4jpZ{BCe3OjeR?= NGPqfHk2OCEQvHevcWw> NHf2Tmc4OiCq NF60WVVKSzVyPUG0MlIh|ryP NHHSSnk6ODJ{N{my
CA755 NV6yNoxVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHfSXHEyOCEQvF2= MofXOkBl M33te2lEPTB;MD6wNVQh|ryP M2LrZlk2OjZ3N{C=
CHO NGLp[VdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV6xNEDPxE1? MnP3OkBl Mn\ETWM2OD1yLkC2JO69VQ>? MXK5OVI3PTdy
Panc02 MmHXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnPZNVAh|ryP MnnrOkBl Mn\STWM2OD1yLkC2OUDPxE1? M3ywcVk2OjZ3N{C=
PC14 M4X0[2N6fG:2b4jpZ{BCe3OjeR?= MYS0JIQ> MlX5SG1UVw>? M4HrUGlEPTB;MD6yPUDPxE1? MkD3PVgzOjV2Mh?=
HOP62 MX;DfZRwfG:6aXOgRZN{[Xl? MXy3NkBp MVfJR|UxRTBwMEC0JO69VQ>? MlGzPVg4OzZ4MR?=
SF-295 NUWxdFlpS3m2b4TvfIlkKEG|c3H5 NHjBN|I4OiCq NU\IXFNGUUN3ME2wMlA{PSEQvF2= M{m3OFk5PzN4NkG=
MDA-MB-361 NIfrW3FEgXSxdH;4bYMhSXO|YYm= NGrreYUyKGh? M1PXV2ROW09? M{DWWmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KE2GQT3NRk0{PjFiZYjwdoV{e2mwZzDMZYNbKHerdHigTWM2OCCxZjCwMlAyOiEQvF2= NWDydlBNOTB|OUW0PVA>
MDA-MB-361 MmnCR5l1d3SxeHnjJGF{e2G7 NHrHe|kyKGh? NXTUV2p1TE2VTx?= NEPlb2tEgXSxdH;4bYNqfHliYXfhbY5{fCCPRFGgUWIhOzZzIHX4dJJme3OrbnegZ4Fz[m:6eYDldJRq\GG|ZTDzeGNRTzJid3n0bEBKSzVyIH;mJFAvODF6IN88US=> MV[xNFM6PTR7MB?=
CCRF-SB NVnTfYFGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M173SVQh\A>? M3fuZmROW09? NGezUHZKSzVyPUCuNFEh|ryP NYTkWVdSOTB2MUG0O|Y>
CHO-K1 M1vkNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4Hmd|Qh\A>? Ml[ySG1UVw>? MmmzTWM2OD1yLkSg{txO NVntSllqOTB2MUG0O|Y>
KB M3XHU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYm0JIQ> MlX0SG1UVw>? M3TLZWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4Sg[JJ2\y2{ZYPpd5RidnRiS1K3SEB4cXSqIFnDOVAhd2ZiMD62OUDPxE1? MX[xNFQyOTR5Nh?=
KB NHf3b4tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVPLcot2PCCm MYXEUXNQ NYDDeI5sSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBlenWpLYLld4l{fGGwdDDLRmNidXC5aYToJGlEPTBib3[gNE4xPiEQvF2= NYHubJFXOTB2MUG0O|Y>
KB NFjKb2NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIPqdIk1KGR? NGXQXnRFVVOR MYrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHTyeYcuemW|aYP0ZY51KEuETVTSJJdqfGhiSVO1NEBw\iByLkWg{txO M17jR|ExPDFzNEe2
KB MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHnQZ5E1KGR? M37rbmROW09? NXfIN5F[SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBlenWpLYLld4l{fGGwdDDLRnYzOENid3n0bEBKSzVyIH;mJFAvPCEQvF2= NXraVHd{OTB2MUG0O|Y>
HTB-54 MXPDfZRwfG:6aXOgRZN{[Xl? NWLoT5ptOTByIN88US=> MXvJR|UxRTFwMTFOwG0> MkmxNVA1PzZ6NkG=
MEL-AC MkHvR5l1d3SxeHnjJGF{e2G7 MorsNVAxKM7:TR?= NFTUbY5KSzVyPUGuNkDPxE1? MVixNFQ4Pjh4MR?=
DU145 NFr4XpNEgXSxdH;4bYMhSXO|YYm= NGjqT4M4OiCq MYLJR|UxRTBwMEWyPEDPxE1? M3G1U|ExPDd7Mkiy
K562R M3S1dWN6fG:2b4jpZ{BCe3OjeR?= MV63NkBp MV\JR|UxRTBwMEG1OkDPxE1? NGm3OVkyODR5OUK4Ni=>
PACA-2 M3riXWN6fG:2b4jpZ{BCe3OjeR?= MnLWOkBl MnjnSWQ2OD1yLkCwOlMh|ryP MXSxNFQ6QDJzNB?=
3Y1 MofOR5l1d3SxeHnjJGF{e2G7 MXK3NkBp MWfHTVUxRTBwMECzPEDPxE1? M1zwZlExPjlzN{Gw
P388 MY\DfZRwfG:6aXOgRZN{[Xl? NU\LOm9VPzJiaB?= M1XKSGdKPTB;MD6wNVIh|ryP NXXqeGltOTB4OUG3NVA>
HMV-1 NF\rVnpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2C5XGlEPTB;MD6yPEDPxE1? MYOxNFY6QDR|Nh?=
MKN-1 MoXHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MU\JR|UxRTBwNkSg{txO M4rlTVExPjl6NEO2
PC14 NHfONlhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXjaRXl[UUN3ME2xMlkh|ryP M1rJTVExPjl6NEO2
T24 MlfIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NELVV2FKSzVyPUCuOlQh|ryP MUOxNFY6QDR|Nh?=
U937 NEfMS41HfW6ldHnvckBCe3OjeR?= MW[yOEBp NYe5W4U4TE2VTx?= M4HtcWlvcGmkaYTzJIlv[2:{cH;yZZRqd25ib3[gX|NJZXSqeX3p[Ilv\SC5aYToJGlEPTBib3[gNE4yKM7:TR?= MYexNFc5ODlzMR?=
H460pv8/eto MkC1R5l1d3SxeHnjJGF{e2G7 NV\pfllFOTByIN88US=> MXG0PEBp NHjDcWtU[WyrbnW= NG[zU|hKSzVyPUCuNFIh|ryP NWjDOmxLOTB5OEC5NVM>
H460pv8 NYDkRpF{S3m2b4TvfIlkKEG|c3H5 Mk[xNVAxKM7:TR?= M135VFQ5KGh? NFfWOZJU[WyrbnW= MlezTWM2OD1yLkCwOEDPxE1? M1LrWVExPzhyOUGz
MDA-231 M2DIUmN6fG:2b4jpZ{BCe3OjeR?= NU\z[|ZzOTByIN88US=> MmDGOFghcA>? M1\lfXNidGmwZR?= MVLDfZRwfG:6aXPpeJkh[WejaX7zeEBOTEFvMkOxJINmdGxiKFXSMUxGT0[UKzzteZRidnRicEWzLUB4cXSqIFnDOVAhd2ZiMD6wNFYh|ryP NET0NnQyODd6MEmxNy=>
MDA-468 NYS5dJA3S3m2b4TvfIlkKEG|c3H5 NVHCXWpZOTByIN88US=> MkSxOFghcA>? Mm\KV4FtcW6n MlrTR5l1d3SxeHnjbZR6KGGpYXnud5QhVUSDLUS2PEBk\WyuIDjFVk0tKGGvcHzp[olm\CCHR1\SMEBufXSjboSgdFU{MSC5aYToJGlEPTBib3[gNE4xODVizszN MVyxNFc5ODlzMx?=
NCI-H226 MoLvR5l1d3SxeHnjJGF{e2G7 NY\GW|ZZOTByIN88US=> NFLRUJM1QCCq MlnYV4FtcW6n NGi1bWZEgXSxdH;4bYNqfHliYXfhbY5{fCCQQ1mtTFIzPiClZXzsJEhufXSjboSgdFU{MSC5aYToJGlEPTBib3[gNE4xODhizszN MVexNFc5ODlzMx?=
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HCC70 Mke4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{jF[2lEPTB;Mj6wOVQ3OyEQvF2= MW\TRW5ITVJ?
SW1088 MmrIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWjkfGpSUUN3ME2yMlA3QTNzIN88US=> MnfpV2FPT0WU
NB6 M3nMOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml\XTWM2OD1{LkG4OUDPxE1? MkfxV2FPT0WU
KU-19-19 NGHLd21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1vHdmlEPTB;Mj6yOlkyPyEQvF2= MVnTRW5ITVJ?
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GMS-10 NHXxc3JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHPCOJlKSzVyPUKuN|k4OzhizszN M12xNnNCVkeHUh?=
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T98G NVSzOZdVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGG3VJdKSzVyPUKuPFA1ODFizszN NXznOHdYW0GQR1XS
DMS-79 NWjpSVYzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mle5TWM2OD1{LkmwPVg5KM7:TR?= NW\CSmV6W0GQR1XS
SNU-475 M1riPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFzHWmFKSzVyPUKuPVI2PzJizszN NHHTcJZUSU6JRWK=
COLO-792 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXfJR|UxRTNwMEK4O{DPxE1? MXrTRW5ITVJ?
YAPC Mm[0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mk\ZTWM2OD1|LkOyPFMzKM7:TR?= MYfTRW5ITVJ?
UACC-812 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFrtXlRKSzVyPUOuN|M4OzhizszN NEPse5ZUSU6JRWK=
Mewo MlPVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoTSTWM2OD1|LkS2NFg3KM7:TR?= MVjTRW5ITVJ?
COLO-824 M1HlNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVzSVYx3UUN3ME2zMlU4Ozd6IN88US=> MU\TRW5ITVJ?
SCC-9 MmPKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3TieGlEPTB;Mz65NVMxQSEQvF2= M2HGXnNCVkeHUh?=
CP50-MEL-B NUKz[2tET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkDPTWM2OD1|Lkm4O|A{KM7:TR?= MlTyV2FPT0WU
NCI-H345 Mlq2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlLwTWM2OD12LkCxNVc1KM7:TR?= Mn;GV2FPT0WU
COR-L88 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGTQdpJKSzVyPUSuN|k2QCEQvF2= MoXtV2FPT0WU
RCM-1 NUT0U3JXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{DXTWlEPTB;ND60PVI4OiEQvF2= NFzMRZpUSU6JRWK=
JVM-3 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1G1U2lEPTB;ND62NFYyQCEQvF2= M3LmOHNCVkeHUh?=
ECC4 NE[2c21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX3mclNpUUN3ME20Mlc3PTRzIN88US=> NWfBcGtuW0GQR1XS
MDA-MB-453 NU[3RmkxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NU\CWoFVUUN3ME20Mlg2OTN2IN88US=> M2LrRXNCVkeHUh?=
SK-N-FI MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEjqO2NKSzVyPUWuNlI5PzdizszN MnnLV2FPT0WU
D-502MG NF3kVZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnTyTWM2OD13LkK0NlYzKM7:TR?= NHT3WVdUSU6JRWK=
NCI-H2227 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M13ZW2lEPTB;NT6zOlI{OSEQvF2= MVXTRW5ITVJ?
DMS-53 NUfVNphJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUK1VYsxUUN3ME22MlE4OTB4IN88US=> M3zjUnNCVkeHUh?=
LB771-HNC MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmnSTWM2OD14LkS2PFA3KM7:TR?= NW\LTVB2W0GQR1XS
BB49-HNC NVfSS3VJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIfsVodKSzVyPUeuOFE4QTFizszN MVrTRW5ITVJ?
MDA-MB-415 NWe4fZdnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{jpPWlEPTB;Nz61O|MxOyEQvF2= MXnTRW5ITVJ?
TALL-1 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV3KXWF5UUN3ME23Mlk3QDhizszN NIfxOnZUSU6JRWK=
HCC1395 NIe4TnVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M171e2lEPTB;OD6xOVEzOiEQvF2= NFXOTllUSU6JRWK=
EFE-184 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV;JR|UxRThwM{eyNVYh|ryP MV\TRW5ITVJ?
EC-GI-10 M1zzV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXfJR|UxRThwNki2PFYh|ryP NVLCNWxLW0GQR1XS
TT NF:w[nJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml64TWM2OD17LkC2PVY3KM7:TR?= M{m1UXNCVkeHUh?=
U-2-OS NFvDb4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mmn2TWM2OD17LkixN|k{KM7:TR?= NH3iN2lUSU6JRWK=
SHP-77 MlH1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV7vdnFGUUN3ME25MlkxOjlizszN MYLTRW5ITVJ?
HCC1143 NGHJUlFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3mxUmlEPTB;MUCuOFc6PyEQvF2= Mmr0V2FPT0WU
A498 NILhb3RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFjMTYhKSzVyPUG4MlI6PzZizszN NHXxN2lUSU6JRWK=
LS-513 Ml\BS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWS3fIVmUUN3ME2xPE43ODl|IN88US=> NGe3NWxUSU6JRWK=
K052 Mn3GS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXfJR|UxRTJzLkS0NVgh|ryP MUPTRW5ITVJ?
SiHa NF33RVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXf0N2FuUUN3ME2yN{4{OzdzIN88US=> NXu0cHlUW0GQR1XS
RCC10RGB NGTiO5ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MknjTWM2OD1|MD6wOlEyKM7:TR?= NYHtT5A3W0GQR1XS
NCI-SNU-16 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUjJR|UxRTNzLkG4N|Yh|ryP M2G1OHNCVkeHUh?=
SNU-C1 MkLZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkH4TWM2OD1|Mj60NVc2KM7:TR?= NX3NVmFoW0GQR1XS
C32 M1\LRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYPJR|UxRTN{Lki3NkDPxE1? MnPHV2FPT0WU
SW684 NX[zcIFIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnXTTWM2OD1|ND6xOVYyKM7:TR?= NV7wdYp3W0GQR1XS
SK-MEL-28 MmHqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXvJR|UxRTN3LkWzO|ch|ryP MWDTRW5ITVJ?
SK-MEL-1 M2jWcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWTJR|UxRTN4Lke1OFch|ryP M33M[XNCVkeHUh?=
NCI-H720 NFXmTnFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NES0XG5KSzVyPUSxMlk4OjZizszN NITwdVhUSU6JRWK=
P31-FUJ NHHIOJFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV\JR|UxRTR2LkOyPFQh|ryP NXvpOGppW0GQR1XS
NCI-H524 NH3QZ2JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M17ZeWlEPTB;NEiuPFI1QCEQvF2= MkfrV2FPT0WU

... Click to View More Cell Line Experimental Data

In vivo In vivo, Doxorubicin in combination with adenoviral MnSOD (AdMnSOD) plus 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) has the greatest effect in decreasing the volumes of MB231 tumors and prolonging survival of mice. [1] Although its use is limited by the chronic and acute toxic side effects it produces, Doxorubicin is essential in treating breast and oesophageal carcinomas, solid tumours in childhood, osteosarcomas, Kaposi's sarcoma, soft tissue sarcomas, and Hodgkin and non-Hodgkin lymphomas. [2]

Protocol

Animal Research:[1]
+ Expand
  • Animal Models: Female athymic nude mice injected s.c. with MB231 cells
  • Formulation: Dissolved in DMSO, and diluted in saline
  • Dosages: 3 mg/kg/day
  • Administration: Delivered intratumorly
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (172.41 mM)
Water 20 mg/mL (34.48 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 579.98
Formula

C27H29NO11.HCl

CAS No. 25316-40-9
Storage powder
Synonyms NSC 123127

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03036904 Not yet recruiting Diffuse Large B-Cell Lymphoma|High Grade B-Cell Lymphoma Weill Medical College of Cornell University|Genentech, Inc.|Massachusetts General Hospital|M.D. Anderson Cancer Center February 6, 2017 Phase 1
NCT02659930 Recruiting Kaposi Sarcoma National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) January 4, 2016 Phase 1
NCT03042819 Not yet recruiting Soft Tissue Sarcoma University Health Network, Toronto|Karyopharm Therapeutics, Inc March 27, 2017 Phase 1
NCT03033914 Recruiting Hodgkin Lymphoma Memorial Sloan Kettering Cancer Center|Bristol-Myers Squibb|M.D. Anderson Cancer Center|City of Hope Medical Center January 25, 2017 Phase 1
NCT02957968 Recruiting Locally Advanced Breast Cancer|Invasive Adenocarcinoma of the Breast|Breast Cancer Stage II|Breast Cancer Stage III|Breast Cancer Stage IV Virginia Commonwealth University|Merck Sharp & Dohme Corp.|National Cancer Institute (NCI) January 24, 2017 Phase 2
NCT02377752 Active, not recruiting Neoplasm Eli Lilly and Company March 23, 2015 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    Why the IC50 of the compound varied over time in our measurement?

  • Answer:

    Sometimes the IC50 value might vary between different batch of compounds due to the slight differences in purity. If different IC50 was seen in the same batch of compound, the problem may lies in the process of dissolvement. Make sure that you see no particles and the solution is clear with your stock solution.

Topoisomerase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID