Doxorubicin (Adriamycin) HCl

Licensed by Pfizer Catalog No.S1208 Synonyms: NSC 123127

Doxorubicin (Adriamycin) HCl Chemical Structure

Molecular Weight(MW): 579.98

Doxorubicin (Adriamycin) HCl is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage and apoptosis in tumor cells.

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Cited by 32 Publications

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  • Enhancement of apoptotic response by addition of targeted agents lapatinib and sunitinib to doxorubicin in the same device reservoir at 24 hours. P values were determined using Student’s t test for all graphs.

    Sci Transl Med, 2015, 7(284): 284ra57 . Doxorubicin (Adriamycin) HCl purchased from Selleck.

    Cell viabilities with increasing concentrations of cisplatin (CP) and doxorubicin (DOXO) under normoxic and hypoxic condition for 48 hours were determined by MTT assay. IC50 values are presented as the means ?SDs (n=4) and * denotes p<0.05.

    Cancer Res 2014 74, 298-308. Doxorubicin (Adriamycin) HCl purchased from Selleck.

  • Cleavage of caspase-3, -9 and the expression of PARP were measured by western blot using b-actin as a loading control.

    Cell Cycle, 2017, 16(5):468-476. Doxorubicin (Adriamycin) HCl purchased from Selleck.

    (C) & (D) Treatments with doxorubicin, cisplatin or AZD6244 further decreased the cell viability in RH30 cloned cells in which STAT3 or GP130 was knockdown by shRNA. (*P<.05, ** P <.01, *** P <.001).

    Curr Cancer Drug Targets, 2016, 16(7):631-8. Doxorubicin (Adriamycin) HCl purchased from Selleck.

  •  

    Doxorubicin release of P(MAA-g-EG) and P(MAA-g-EG) containing nanoparticles in two-step pH conditions. Doxorubicinloaded P(MAA-g-EG) ,P(MAA-g-EG)-1.0NP ,P(MAA-g-EG)-2.5NP, and P(MAA-g-EG)-5.0NP crushed particles (75–150 um) were released in 1 PBS (pH 2.0) for 90 min. Then the pH was increased to 7.0 by adding 5N NaOH and release continued for 6 h.

    J Biomed Mater Res Part A 2012 10.1002/jbm.a.34064. Doxorubicin (Adriamycin) HCl purchased from Selleck.

    Temporal uptake of free DOX or DOX-PCL63-b-PNVP90 micelles into K-562 (I) and DL (J) cells were studied by incubating in 24-well plates at a concentration of 50,000 cells per well. Representative images shown were obtained using a fluorescence microscope Eclipse 80i (Nikon, Japan) (Plan Fluor, 40X, NA 0.75 objective) equipped with blue and red filters for, Hoechst and Doxorubicin respectively (n = 3).

    PLoS One 2014 9, e94309. Doxorubicin (Adriamycin) HCl purchased from Selleck.

  • PLoS One 2013 8(1), e54595. Doxorubicin (Adriamycin) HCl purchased from Selleck.

    CON+ -positive control (with FBS as chemoattractant); CON- - negative control (without chemoattractant); DOX -doxorubicin; WZ -WZ811; PF- PF-573228; * -significant difference compared to positive control: p<0.05; ** -significant difference compared to positive control: p<0.01; # -significant difference between combination and inhibitor only: p<0.05. Cancer cell invasion was analyzed using Transwell membranes assembled in 24-well plates and covered with a layer of Matrigel. COR-L23 cells were treated for 24 h with 50 nM DOX, 10 uM WZ811, 5 uM PF-573228 and combinations of inhibitors with 50 nM DOX. Similarly, RH460 cells were treated for 24 h with 1 uM DOX, 10 uM WZ811, 2.5 uM PF-573228 and combinations of inhibitors with 1 uM DOX. Applied doses were the ones that exerted the most pronounced synergistic effect on cytotoxicity level. After the incubation period, cells that invaded through matrigel and membranes were fixed in 4% paraformaldehyde-PBS and stained with Hoechst 33342 nuclear stain. Cells were visualized and counted under a fluorescent microscope at 10x magnification.

    2014 Dr Milica Pesic from Institute for Biological Research. Doxorubicin (Adriamycin) HCl purchased from Selleck.

Purity & Quality Control

Choose Selective Topoisomerase Inhibitors

Biological Activity

Description Doxorubicin (Adriamycin) HCl is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage and apoptosis in tumor cells.
Targets
Topo II [1]
(Tumor cells)
In vitro

Doxorubicin, an antibiotic anthracycline, is commonly considered to exert its anti-tumor activity at two fundamental levels, altering DNA and producing free radicals to trigger apoptosis of cancer cells through DNA damage. Doxorubicin can block the synthesis of DNA by intercalating into the DNA strand, and inhibits DNA topoisomerase II (TOP2). Doxorubicin is most effective when cells are rapidly proliferating and expressing high levels of TOP2. Additionally, Doxorubicin can trigger apoptosis by producing ceramide (which prompts apoptosis by activating p53 or other downstream pathways such as JNK), the degradation of Akt by serine threonine proteases, the mitochondrial release of cytochrome c, increased FasL (death receptor Fas/CD95 ligand) mRNA production, and a greater production of free radicals. [2] Pre-treatment with GSNO (nitrosoglutathione) suppresses the resistance in the doxorubicin-resistant breast cancer cell line MCF7/Dx, accompanied by enhanced protein glutathionylation and accumulation of doxorubicin in the nucleus. [3] Doxorubicin induced G2/M checkpoint arrest are attributed to elevated cyclin G2 (CycG2) expression and phospho-modification of proteins in the ataxia telangiectasia mutated (ATM) and ATM and Rad3-related (ATR) signaling pathways. [5] Doxorubicin inhibits AMP-activated protein kinase (AMPK), resulting in SIRT1 dysfunction, p53 accumulation, and increased cell death in mouse embryonic fibroblasts (MEFs) and cardiomyocytes, which can be further sensitized by pre-inhibition of AMPK. [6] Doxorubicin elicits a marked heat shock response, and that either inhibition or silencing of heat shock proteins enhance the Doxorubicin apoptotic effect in neuroblastoma cells. Nanomolar Doxorubicin treatment of neuroblastoma cells causes dose-dependent over-ubiquitination of a specific set of proteins in the absence of measurable inhibition of proteasome, and loss of activity of ubiquitinated enzymes such as lactate dehydrogenase and α-enolase, the protein ubiquination patterns of which is similar to those with proteasome inhibitor Bortezomib, indicating that Doxorubicin may also exert its effect by damaging proteins. [8]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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H9 NVHjbmVXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWLJcohq[mm2aX;uJI9nKEh7IHPlcIx{KGOqcn;ubYNidGy7IHnu[oVkfGWmIIfpeIghfGinIFjJWk0yKCiKOT;JTWlDMSC5aYToJGlFPTBib3[gNE4xOSEQvF2= NVTEVJRzOTV2OE[4NS=>
T-C8166 NHrXZWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHTJXVdKTDVyPUCuNFEh|ryP NVjuNVdlOTV2OE[4NS=>
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T-MT4 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlzJTWQ2OD1yLkCxJO69VQ>? M1S5OlE2PDh4OEG=
SKMEL-5 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmjMSWQ2OD1|LkKg{txO NVPCXmZOOTZzM{e1Ny=>
MLM M4LuS2N6fG:2b4jpZ{BCe3OjeR?= Mn:wSWQ2OD1yLkGzJO69VQ>? Ml76NVg4PTN3MB?=
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HT-29 NEjmfHhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGLHTWYxNjBzIN88[y=> NWfYSmplPDhiaB?= M1vYSGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5 MnW0Nlc4QDR2OR?=
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H2981 Mon3R5l1d3SxeHnjJGF{e2G7 M2fFZ2lEPTB;MD61JO69VQ>? M4j5NVc4OzFyMkO=
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C38 M4DEPGN6fG:2b4jpZ{BCe3OjeR?= MW\JR|UxRTBwMEGg{txO MUK4NFU4Ojlz
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MCF NX;FV4xWTnWwY4Tpc44hSXO|YYm= MojDVoV3\XK|YXygc4YhWC2pcD3t[YRq[XSnZDDteYx1cWS{dXegdoV{cXO2YX7j[UBie3Onc4Pl[EBieyCnbnjhcoNmdWWwdDDpckBkgXSxdH;4bYNqfHlib3[gZYRzcWGveXPpci=> M1v6R|g6QDRzNUG=
C6 MoPPR5l1d3SxeHnjJGF{e2G7 MnnpNk42KG2pL33M NI[wfHQ4OiCq MmHrSG1UVw>? MUPDfZRwfG:6aXPpeJkh[WejaX7zeEBz[XRiQ{[gZ4VtdHNiYYSgOVAhfWdxbVygeI8hOi53IH3nM41N M2Lp[lg6QDh4MES=
C6 M{S1UmZ2dmO2aX;uJGF{e2G7 NWHhWlQ4Oi53IH3nM41N MmPHOEBp MXXEUXNQ M{XSPWlvcGmkaYTpc44hd2ZidIXieYxqdiCyb3z5cYVzcXqjdHnvci=> M2jNUFg6QDh4MES=
C6 MWLDfZRwfG:6aXOgRZN{[Xl? MV2yMlUhdWdxbVy= MnnsOUBp MnXBSG1UVw>? NWKzXmdzS3m2b4TvfIlkcXS7IHHzd4V{e2WmIHHzJINmdGxicnXs[YF{\Q>? M2ntVVg6QDh4MES=
SKVLB NETxZ2ZEgXSxdH;4bYMhSXO|YYm= MnfpO|IhcA>? MmDYTWM2OD14LkW0JO69VQ>? MnHRPVAxOzV{MB?=
MCF7/VP16 NGDYc3FEgXSxdH;4bYMhSXO|YYm= MnPBOVAh|rypL33M M{DLd|czKGh? MUTJR|UxRTF2LkKg{txO MY[5NFIzPzl{
CA755 M1PBPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2rCOFExKM7:TR?= MXy2JIQ> MmrlTWM2OD1yLkCxOEDPxE1? M3vnW|k2OjZ3N{C=
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Panc02 Ml\XS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVuxNEDPxE1? NF3tUpA3KGR? M4fNcmlEPTB;MD6wOlUh|ryP MV65OVI3PTdy
PC14 NXf3dVR{S3m2b4TvfIlkKEG|c3H5 MXG0JIQ> NEnOWVdFVVOR NXjSd|Q4UUN3ME2wMlI6KM7:TR?= MW[5PFIzPTR{
HOP62 MlryR5l1d3SxeHnjJGF{e2G7 Ml3IO|IhcA>? MmLvTWM2OD1yLkCwOEDPxE1? Ml[zPVg4OzZ4MR?=
SF-295 MoHLR5l1d3SxeHnjJGF{e2G7 MWq3NkBp MUHJR|UxRTBwMEO1JO69VQ>? M1LZO|k5PzN4NkG=
MDA-MB-361 NHHBPJpEgXSxdH;4bYMhSXO|YYm= NGHOSpEyKGh? Mn7FSG1UVw>? NH:1NnZEgXSxdH;4bYNqfHliYXfhbY5{fCCPRFGtUWIuOzZzIHX4dJJme3OrbnegUIFkYiC5aYToJGlEPTBib3[gNE4xOTJizszN MmO5NVA{QTV2OUC=
MDA-MB-361 M3ezTmN6fG:2b4jpZ{BCe3OjeR?= MUWxJIg> M1nCVGROW09? M1LHXGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KE2GQTDNRkA{PjFiZYjwdoV{e2mwZzDjZZJjd3i7cHXweIll[XOnIIP0R3BIOiC5aYToJGlEPTBib3[gNE4xOThizszN NWDlTZhUOTB|OUW0PVA>
CCRF-SB MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M17jWFQh\A>? M2DP[WROW09? NE\4dphKSzVyPUCuNFEh|ryP MWSxNFQyOTR5Nh?=
CHO-K1 NEPySYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mmf1OEBl MVrEUXNQ MkDXTWM2OD1yLkSg{txO NGnZUYgyODRzMUS3Oi=>
KB NFX6eWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MljPOEBl NH63TolFVVOR NY[zT5F6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBlenWpLYLld4l{fGGwdDDLRldFKHerdHigTWM2OCCxZjCwMlY2KM7:TR?= MVOxNFQyOTR5Nh?=
KB NHPufYhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVO0JIQ> NFrGPWlFVVOR M2PmemFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4Sg[JJ2\y2{ZYPpd5RidnRiS1LDZY1xf2m2aDDJR|UxKG:oIECuNFYh|ryP NHPYTlMyODRzMUS3Oi=>
KB NYK2XWJKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkHxOEBl NXPhXItbTE2VTx?= MVHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHTyeYcuemW|aYP0ZY51KEuETVTSJJdqfGhiSVO1NEBw\iByLkWg{txO NHzDfZAyODRzMUS3Oi=>
KB NFXXRZJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVK0JIQ> M3\lPWROW09? M3XzXGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4Sg[JJ2\y2{ZYPpd5RidnRiS1LWNlBEKHerdHigTWM2OCCxZjCwMlQh|ryP NVzt[JNIOTB2MUG0O|Y>
HTB-54 NWf6UWVHS3m2b4TvfIlkKEG|c3H5 NXLubmdwOTByIN88US=> NVX5N3J2UUN3ME2xMlEh|ryP NUPxepJJOTB2N{[4OlE>
MEL-AC MlvXR5l1d3SxeHnjJGF{e2G7 MmKyNVAxKM7:TR?= NX;FeHhRUUN3ME2xMlIh|ryP MV:xNFQ4Pjh4MR?=
DU145 MVvDfZRwfG:6aXOgRZN{[Xl? NH3zbHQ4OiCq MV\JR|UxRTBwMEWyPEDPxE1? M{iwWlExPDd7Mkiy
K562R M4X0OGN6fG:2b4jpZ{BCe3OjeR?= M2HJZVczKGh? NHHHV|dKSzVyPUCuNFE2PiEQvF2= MYGxNFQ4QTJ6Mh?=
PACA-2 MX3DfZRwfG:6aXOgRZN{[Xl? MnzKOkBl NIT0RpNGTDVyPUCuNFA3OyEQvF2= MmTtNVA1QTh{MUS=
3Y1 M{HORWN6fG:2b4jpZ{BCe3OjeR?= NUHVd4YzPzJiaB?= NWnwWIp{T0l3ME2wMlAxOzhizszN MWixNFY6OTdzMB?=
P388 MW\DfZRwfG:6aXOgRZN{[Xl? M{[zTFczKGh? M2W5fmdKPTB;MD6wNVIh|ryP MVGxNFY6OTdzMB?=
HMV-1 M4jn[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYLjfY1IUUN3ME2wMlI5KM7:TR?= MlnyNVA3QTh2M{[=
MKN-1 NVjVOWlmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX;JR|UxRTBwNkSg{txO NFzWNVEyODZ7OESzOi=>
PC14 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml:4TWM2OD1zLkmg{txO MYOxNFY6QDR|Nh?=
T24 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3zwZ2lEPTB;MD62OEDPxE1? NWTMcGREOTB4OUi0N|Y>
U937 NEDrXXBHfW6ldHnvckBCe3OjeR?= MlqxNlQhcA>? NITpUXFFVVOR M4jXZmlvcGmkaYTzJIlv[2:{cH;yZZRqd25ib3[gX|NJZXSqeX3p[Ilv\SC5aYToJGlEPTBib3[gNE4yKM7:TR?= Mn35NVA4QDB7MUG=
H460pv8/eto NHPIcVVEgXSxdH;4bYMhSXO|YYm= MnS5NVAxKM7:TR?= NF;YeVU1QCCq MYXTZYxqdmV? NWT5ZpVXUUN3ME2wMlAzKM7:TR?= M{fWTlExPzhyOUGz
H460pv8 MXzDfZRwfG:6aXOgRZN{[Xl? NX7tSFZXOTByIN88US=> NHX2[oE1QCCq MmDVV4FtcW6n MlLGTWM2OD1yLkCwOEDPxE1? MorlNVA4QDB7MUO=
MDA-231 M2jqO2N6fG:2b4jpZ{BCe3OjeR?= MWCxNFAh|ryP NVf4cm9uPDhiaB?= MYHTZYxqdmV? MYDDfZRwfG:6aXPpeJkh[WejaX7zeEBOTEFvMkOxJINmdGxiKFXSMUxGT0[UKzzteZRidnRicEWzLUB4cXSqIFnDOVAhd2ZiMD6wNFYh|ryP NFnpTW4yODd6MEmxNy=>
MDA-468 NHzYNpFEgXSxdH;4bYMhSXO|YYm= NELrbZgyODBizszN M{fCUFQ5KGh? MkDYV4FtcW6n NWPMSmloS3m2b4TvfIlkcXS7IHHnZYlve3RiTVTBMVQ3QCClZXzsJEhGWi1uIHHtdIxq\mmnZDDFS2ZTNCCvdYThcpQheDV|KTD3bZRpKEmFNUCgc4YhOC5yMEWg{txO M16yVVExPzhyOUGz
NCI-H226 MXrDfZRwfG:6aXOgRZN{[Xl? NUntc|E3OTByIN88US=> MnK4OFghcA>? NY[wZ5oxW2GuaX7l M1XxT2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KE6FST3INlI3KGOnbHygLI12fGGwdDDwOVMqKHerdHigTWM2OCCxZjCwMlAxQCEQvF2= MoD6NVA4QDB7MUO=
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GT3TKB NEnxXHNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{iwWGlEPTB;NkeuNVkhdk1? MlTKV2FPT0WU
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MC-IXC MkHrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUH2[lNLUUN3ME22O{46KG6P NH3nbY5USU6JRWK=
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A2780 M120S2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUfCeXo6UUN3ME22PE44QSCwTR?= NFPEcm5USU6JRWK=
Daudi M33SO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYnJR|UxRTZ6Lkm2JI5O MkPmV2FPT0WU
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AN3-CA MofPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3PsVWlEPTB;N{KuN|Ihdk1? Mn\MV2FPT0WU
HCC1599 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3X6[mlEPTB;N{KuOVQhdk1? NYPvdXJjW0GQR1XS
HuCCT1 NX\MO2JVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MU\JR|UxRTd{LkmzJI5O M4HpR3NCVkeHUh?=
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IST-SL2 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1rMXWlEPTB;N{OuPFEhdk1? MkLuV2FPT0WU
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NH-12 NGK0ToRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVvJR|UxRTd4IH7N M13sZXNCVkeHUh?=
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TUR MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYXJR|UxRTd4LkOyJI5O MVjTRW5ITVJ?
TE-1 NY[3OHNJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYf1fVI1UUN3ME23Ok4{PSCwTR?= NHrOfFBUSU6JRWK=
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LB996-RCC MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml;6TWM2OD15OD60OUBvVQ>? MkPCV2FPT0WU
NCI-H1650 MmnKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEDlboJKSzVyPUe4MlYzKG6P NGnjfVFUSU6JRWK=
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A172 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYHJR|UxRThyLkW1JI5O NH7Ob3NUSU6JRWK=
TE-12 NHHaWolIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4XDV2lEPTB;OEGuNFYhdk1? NF\aPHVUSU6JRWK=
MKN45 MkTRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXLVVlAxUUN3ME24NU42OSCwTR?= Mo\iV2FPT0WU
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ETK-1 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmnFTWM2OD16Mz6wNUBvVQ>? NVnyNFZUW0GQR1XS
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PANC-10-05 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIXrc3ZKSzVyPUi4MlQ2KG6P NE\DSpdUSU6JRWK=
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TE-441-T M3npSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHftV41KSzVyPUi5MlgzKG6P NX;4S2t5W0GQR1XS
KM-H2 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGLQcmxKSzVyPUmwMlY5KG6P NVnhcGF2W0GQR1XS
SH-4 NYPvXXZsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWHxfFhuUUN3ME25Nk4yOSCwTR?= NFPsWYdUSU6JRWK=
SF268 NVrlVZhGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3XFPGlEPTB;OUKuOFIhdk1? NWS1fmp7W0GQR1XS
VMRC-RCZ MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn;tTWM2OD17Mz6yOkBvVQ>? NUTpPG1kW0GQR1XS
GI-ME-N NWjENIlYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWDsW2YyUUN3ME25N{4{OyCwTR?= NVuwcoZ3W0GQR1XS
JEG-3 M3fpcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3P2RmlEPTB;OUWuNVIhdk1? NEX2SZVUSU6JRWK=
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K5 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFWxOYlKSzVyPUm2Mlkhdk1? NYPMU|lrW0GQR1XS
EFO-27 NF\ae3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnfuTWM2OD17Nz60OEBvVQ>? Mn;YV2FPT0WU
SW1783 NUL0NYdiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIrQeZlKSzVyPUm3Mlk6KG6P M1jSW3NCVkeHUh?=
CAL-39 MmrUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYrudnd[UUN3ME25PE4zPCCwTR?= NG\jRZlUSU6JRWK=
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G-402 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWnJR|UxRTl7LkC5JI5O Mnj3V2FPT0WU
MES-SA M4TSc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXPYc2JJUUN3ME25PU42OyCwTR?= MU\TRW5ITVJ?
FADU NFHKU2xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn3ZTWM2OD1zMEKuNVghdk1? NFHNd2NUSU6JRWK=
DJM-1 NXXNVXhzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV3JR|UxRTFyMj6yOUBvVQ>? MnWyV2FPT0WU
SU-DHL-1 MnfaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnnJTWM2OD1zMEKuOUBvVQ>? MVfTRW5ITVJ?
KYSE-410 M1[xNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV;3d2R5UUN3ME2xNFMvPDZibl2= M3[2dHNCVkeHUh?=
NCI-H1618 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnPwTWM2OD1zMESuN|Uhdk1? M4f1XXNCVkeHUh?=
AU565 NGn0TYtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX7ofI9UUUN3ME2xNFQvPTFibl2= NXnDO5VnW0GQR1XS
SF126 MnXqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF;ZfotKSzVyPUGwOE42QCCwTR?= M3rlWHNCVkeHUh?=
MMAC-SF Ml64S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUCwe25QUUN3ME2xNFYvPDhibl2= NIjMXIpUSU6JRWK=
ChaGo-K-1 NFzsRoJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYrOUnpEUUN3ME2xNFcvQTVibl2= MUPTRW5ITVJ?
NCI-H596 MmfrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{PBN2lEPTB;MUC4MlAyKG6P M2jEWnNCVkeHUh?=
LXF-289 NXzUcIxsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWDaSZRXUUN3ME2xNFkvOjRibl2= NIDXNIZUSU6JRWK=
HCC1569 NH72e45Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmfpTWM2OD1zMEmuN|Qhdk1? MlvwV2FPT0WU
NCI-H2170 MljxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoXGTWM2OD1zMEmuPVIhdk1? NHvzc|dUSU6JRWK=
ARH-77 MoCwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX3JR|UxRTFyOT65PUBvVQ>? MWXTRW5ITVJ?
BOKU MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mnv6TWM2OD1zMUCuPFchdk1? MVjTRW5ITVJ?
SJSA-1 NGLIZW9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXHNeJcyUUN3ME2xNVEvODVibl2= M2jTbnNCVkeHUh?=
SW626 NIjEemdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mmn5TWM2OD1zMUGuO|Ehdk1? NEm4dHBUSU6JRWK=
EHEB NYXLb486T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWPJR|UxRTFzMj61PUBvVQ>? MWDTRW5ITVJ?
TE-10 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn[wTWM2OD1zMUKuOlghdk1? M3LhdXNCVkeHUh?=
MZ7-mel MmfwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGDWfI5KSzVyPUGxNk45PCCwTR?= NVvDb2hWW0GQR1XS
SAS NFPlRYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXP2b3NkUUN3ME2xNVQvPDVibl2= Mk\DV2FPT0WU
TK10 M1nldWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MljaTWM2OD1zMUSuOkBvVQ>? MXrTRW5ITVJ?
SW13 NU\ISIRjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUPPdYp4UUN3ME2xNVUvOjZibl2= NXPxNFI2W0GQR1XS
HCC1937 MnrkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVXJR|UxRTFzNT64OEBvVQ>? NHTVRpNUSU6JRWK=
SK-NEP-1 MlrnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGToSFJKSzVyPUGxOk4zPiCwTR?= MmeyV2FPT0WU
OVCAR-5 M1jReGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NE\ndldKSzVyPUGxOk45OSCwTR?= M{joO3NCVkeHUh?=
SW837 Mm\VS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVXJR|UxRTFzNz65OUBvVQ>? Mo\hV2FPT0WU
BFTC-909 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWTE[HZoUUN3ME2xNVgvPDhibl2= M1K0R3NCVkeHUh?=
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SF539 NEftTJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH;CT|lKSzVyPUGyNU44OiCwTR?= MmnSV2FPT0WU
MLMA M3rSTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHHPSHVKSzVyPUGyNk4xQCCwTR?= NXrRVGwzW0GQR1XS
BL-41 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoHNTWM2OD1zMkKuNlIhdk1? NVvOZoRkW0GQR1XS
LB647-SCLC NUTEc2xiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUjJR|UxRTF{Mz6zOUBvVQ>? NFnPNZlUSU6JRWK=
NCI-H1703 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{S4d2lEPTB;MUK2MlQzKG6P MoXyV2FPT0WU
KURAMOCHI MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWHJR|UxRTF{Nj63OkBvVQ>? NHiwWGlUSU6JRWK=
ATN-1 NV3BS3ZNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYrJR|UxRTF{Nz63JI5O NITLUFBUSU6JRWK=
SK-CO-1 Ml:3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2rnS2lEPTB;MUK5MlIyKG6P MlXNV2FPT0WU
RPMI-7951 NX[yWJdkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmfnTWM2OD1zM{CuNVQhdk1? MlrwV2FPT0WU
NCI-H1648 M2W3Tmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mm\UTWM2OD1zM{GuPVQhdk1? MnrvV2FPT0WU
NCI-H64 NFnJS|NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXvWRndnUUN3ME2xN|IvPjVibl2= M16zc3NCVkeHUh?=
HT-3 NHznSJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2GxSGlEPTB;MUOzMlA1KG6P NHj5PY5USU6JRWK=
NCI-H1770 NVrsW|czT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYXPeXdzUUN3ME2xN|MvOjFibl2= NF:0dHBUSU6JRWK=
NB13 NE\xZmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFfUSIhKSzVyPUGzOE42PSCwTR?= M1;yR3NCVkeHUh?=
NCI-H747 MknoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV3SVlE{UUN3ME2xN|UvODJibl2= M4m5THNCVkeHUh?=
ML-2 Mnf4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYnTboxUUUN3ME2xN|UvOTZibl2= MVjTRW5ITVJ?
CA46 M2nITGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIThWo1KSzVyPUGzOk45QCCwTR?= MYTTRW5ITVJ?
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JiyoyeP-2003 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYrJR|UxRTF|Nz64JI5O M1zXW3NCVkeHUh?=
NMC-G1 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3;tUmlEPTB;MUO5MlE{KG6P MV7TRW5ITVJ?
U-698-M NVrGb5RQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIfaTplKSzVyPUGzPU42KG6P MWHTRW5ITVJ?
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NCI-H748 NVu1d3BHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVLJfHkxUUN3ME2xOFcvOzFibl2= M3\PbHNCVkeHUh?=
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SNB75 NUnBN5JZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlTpTWM2OD1zNEiuOlUhdk1? Mn\CV2FPT0WU
BB65-RCC NEjmd41Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUXJR|UxRTF2OT6zNUBvVQ>? NF62T3VUSU6JRWK=
NCI-H1355 MknFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVLJR|UxRTF3Mj6yOEBvVQ>? NHGwco1USU6JRWK=
SCC-25 M4nRSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX3zPFFkUUN3ME2xOVMvPzNibl2= MUnTRW5ITVJ?
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MONO-MAC-6 NYXvV2s3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlT4TWM2OD1zNUSuN|Yhdk1? Mn\tV2FPT0WU
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UACC-812 M3\5S2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnS5TWM2OD1|LkOzO|M5KM7:TR?= MVjTRW5ITVJ?
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COLO-824 NI\XbolIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXLJR|UxRTNwNUezO|gh|ryP M4PBenNCVkeHUh?=
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COR-L88 NYXrdFJET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX;JR|UxRTRwM{m1PEDPxE1? MoD0V2FPT0WU
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D-502MG NXj5dHZ[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUjvZ29sUUN3ME21MlI1OjZ{IN88US=> MW\TRW5ITVJ?
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DMS-53 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXrJR|UxRTZwMUexNFYh|ryP MX7TRW5ITVJ?
LB771-HNC NV23emoyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnzTTWM2OD14LkS2PFA3KM7:TR?= MljPV2FPT0WU
BB49-HNC NHT1ZVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF7RSllKSzVyPUeuOFE4QTFizszN MWrTRW5ITVJ?
MDA-MB-415 NFzDZXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3q1dmlEPTB;Nz61O|MxOyEQvF2= NGPOeVFUSU6JRWK=
TALL-1 NEDKeIlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYnsOop1UUN3ME23Mlk3QDhizszN M1:zXXNCVkeHUh?=
HCC1395 NHz6N4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUj6XXZIUUN3ME24MlE2OTJ{IN88US=> M17N[HNCVkeHUh?=
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C32 NHvZUZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVLJR|UxRTN{Lki3NkDPxE1? NHHZN5pUSU6JRWK=
SW684 NVzyZ|RkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1GxVmlEPTB;M{SuNVU3OSEQvF2= MXzTRW5ITVJ?
SK-MEL-28 NUnHeYh1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4LYeWlEPTB;M{WuOVM4PyEQvF2= NFHuTmpUSU6JRWK=
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NCI-H524 M332Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUfJcIQ4UUN3ME20PE45OjR6IN88US=> MlHWV2FPT0WU

... Click to View More Cell Line Experimental Data

In vivo In vivo, Doxorubicin in combination with adenoviral MnSOD (AdMnSOD) plus 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) has the greatest effect in decreasing the volumes of MB231 tumors and prolonging survival of mice. [1] Although its use is limited by the chronic and acute toxic side effects it produces, Doxorubicin is essential in treating breast and oesophageal carcinomas, solid tumours in childhood, osteosarcomas, Kaposi's sarcoma, soft tissue sarcomas, and Hodgkin and non-Hodgkin lymphomas. [2]

Protocol

Animal Research:[1]
+ Expand
  • Animal Models: Female athymic nude mice injected s.c. with MB231 cells
  • Formulation: Dissolved in DMSO, and diluted in saline
  • Dosages: 3 mg/kg/day
  • Administration: Delivered intratumorly
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (172.41 mM)
Water 20 mg/mL (34.48 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 579.98
Formula

C27H29NO11.HCl

CAS No. 25316-40-9
Storage powder
in solvent
Synonyms NSC 123127

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03036904 Not yet recruiting Diffuse Large B-Cell Lymphoma|High Grade B-Cell Lymphoma Weill Medical College of Cornell University|Genentech, Inc.|Massachusetts General Hospital|M.D. Anderson Cancer Center February 6, 2017 Phase 1
NCT02659930 Recruiting Kaposi Sarcoma National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) January 4, 2016 Phase 1
NCT03042819 Not yet recruiting Soft Tissue Sarcoma University Health Network, Toronto|Karyopharm Therapeutics, Inc March 27, 2017 Phase 1
NCT03033914 Recruiting Hodgkin Lymphoma Memorial Sloan Kettering Cancer Center|Bristol-Myers Squibb|M.D. Anderson Cancer Center|City of Hope Medical Center January 25, 2017 Phase 1
NCT02957968 Recruiting Locally Advanced Breast Cancer|Invasive Adenocarcinoma of the Breast|Breast Cancer Stage II|Breast Cancer Stage III|Breast Cancer Stage IV Virginia Commonwealth University|Merck Sharp & Dohme Corp.|National Cancer Institute (NCI) January 24, 2017 Phase 2
NCT02377752 Active, not recruiting Neoplasm Eli Lilly and Company March 23, 2015 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    Why the IC50 of the compound varied over time in our measurement?

  • Answer:

    Sometimes the IC50 value might vary between different batch of compounds due to the slight differences in purity. If different IC50 was seen in the same batch of compound, the problem may lies in the process of dissolvement. Make sure that you see no particles and the solution is clear with your stock solution.

Topoisomerase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID