Doxorubicin (Adriamycin) HCl

Licensed by Pfizer Catalog No.S1208 Synonyms: NSC 123127

Doxorubicin (Adriamycin) HCl Chemical Structure

Molecular Weight(MW): 579.98

Doxorubicin (Adriamycin) HCl is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage and apoptosis in tumor cells.

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Cited by 31 Publications

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  • Enhancement of apoptotic response by addition of targeted agents lapatinib and sunitinib to doxorubicin in the same device reservoir at 24 hours. P values were determined using Student’s t test for all graphs.

    Sci Transl Med, 2015, 7(284): 284ra57 . Doxorubicin (Adriamycin) HCl purchased from Selleck.

    Cell viabilities with increasing concentrations of cisplatin (CP) and doxorubicin (DOXO) under normoxic and hypoxic condition for 48 hours were determined by MTT assay. IC50 values are presented as the means ?SDs (n=4) and * denotes p<0.05.

    Cancer Res 2014 74, 298-308. Doxorubicin (Adriamycin) HCl purchased from Selleck.

  • Cleavage of caspase-3, -9 and the expression of PARP were measured by western blot using b-actin as a loading control.

    Cell Cycle, 2017.. Doxorubicin (Adriamycin) HCl purchased from Selleck.

    (C) & (D) Treatments with doxorubicin, cisplatin or AZD6244 further decreased the cell viability in RH30 cloned cells in which STAT3 or GP130 was knockdown by shRNA. (*P<.05, ** P <.01, *** P <.001).

    Curr Cancer Drug Targets, 2016, 16(7):631-8. Doxorubicin (Adriamycin) HCl purchased from Selleck.

  •  

    Doxorubicin release of P(MAA-g-EG) and P(MAA-g-EG) containing nanoparticles in two-step pH conditions. Doxorubicinloaded P(MAA-g-EG) ,P(MAA-g-EG)-1.0NP ,P(MAA-g-EG)-2.5NP, and P(MAA-g-EG)-5.0NP crushed particles (75–150 um) were released in 1 PBS (pH 2.0) for 90 min. Then the pH was increased to 7.0 by adding 5N NaOH and release continued for 6 h.

    J Biomed Mater Res Part A 2012 10.1002/jbm.a.34064. Doxorubicin (Adriamycin) HCl purchased from Selleck.

    Temporal uptake of free DOX or DOX-PCL63-b-PNVP90 micelles into K-562 (I) and DL (J) cells were studied by incubating in 24-well plates at a concentration of 50,000 cells per well. Representative images shown were obtained using a fluorescence microscope Eclipse 80i (Nikon, Japan) (Plan Fluor, 40X, NA 0.75 objective) equipped with blue and red filters for, Hoechst and Doxorubicin respectively (n = 3).

    PLoS One 2014 9, e94309. Doxorubicin (Adriamycin) HCl purchased from Selleck.

  • PLoS One 2013 8(1), e54595. Doxorubicin (Adriamycin) HCl purchased from Selleck.

    CON+ -positive control (with FBS as chemoattractant); CON- - negative control (without chemoattractant); DOX -doxorubicin; WZ -WZ811; PF- PF-573228; * -significant difference compared to positive control: p<0.05; ** -significant difference compared to positive control: p<0.01; # -significant difference between combination and inhibitor only: p<0.05. Cancer cell invasion was analyzed using Transwell membranes assembled in 24-well plates and covered with a layer of Matrigel. COR-L23 cells were treated for 24 h with 50 nM DOX, 10 uM WZ811, 5 uM PF-573228 and combinations of inhibitors with 50 nM DOX. Similarly, RH460 cells were treated for 24 h with 1 uM DOX, 10 uM WZ811, 2.5 uM PF-573228 and combinations of inhibitors with 1 uM DOX. Applied doses were the ones that exerted the most pronounced synergistic effect on cytotoxicity level. After the incubation period, cells that invaded through matrigel and membranes were fixed in 4% paraformaldehyde-PBS and stained with Hoechst 33342 nuclear stain. Cells were visualized and counted under a fluorescent microscope at 10x magnification.

    2014 Dr Milica Pesic from Institute for Biological Research. Doxorubicin (Adriamycin) HCl purchased from Selleck.

Purity & Quality Control

Choose Selective Topoisomerase Inhibitors

Biological Activity

Description Doxorubicin (Adriamycin) HCl is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage and apoptosis in tumor cells.
Targets
Topo II [1]
(Tumor cells)
In vitro

Doxorubicin, an antibiotic anthracycline, is commonly considered to exert its anti-tumor activity at two fundamental levels, altering DNA and producing free radicals to trigger apoptosis of cancer cells through DNA damage. Doxorubicin can block the synthesis of DNA by intercalating into the DNA strand, and inhibits DNA topoisomerase II (TOP2). Doxorubicin is most effective when cells are rapidly proliferating and expressing high levels of TOP2. Additionally, Doxorubicin can trigger apoptosis by producing ceramide (which prompts apoptosis by activating p53 or other downstream pathways such as JNK), the degradation of Akt by serine threonine proteases, the mitochondrial release of cytochrome c, increased FasL (death receptor Fas/CD95 ligand) mRNA production, and a greater production of free radicals. [2] Pre-treatment with GSNO (nitrosoglutathione) suppresses the resistance in the doxorubicin-resistant breast cancer cell line MCF7/Dx, accompanied by enhanced protein glutathionylation and accumulation of doxorubicin in the nucleus. [3] Doxorubicin induced G2/M checkpoint arrest are attributed to elevated cyclin G2 (CycG2) expression and phospho-modification of proteins in the ataxia telangiectasia mutated (ATM) and ATM and Rad3-related (ATR) signaling pathways. [5] Doxorubicin inhibits AMP-activated protein kinase (AMPK), resulting in SIRT1 dysfunction, p53 accumulation, and increased cell death in mouse embryonic fibroblasts (MEFs) and cardiomyocytes, which can be further sensitized by pre-inhibition of AMPK. [6] Doxorubicin elicits a marked heat shock response, and that either inhibition or silencing of heat shock proteins enhance the Doxorubicin apoptotic effect in neuroblastoma cells. Nanomolar Doxorubicin treatment of neuroblastoma cells causes dose-dependent over-ubiquitination of a specific set of proteins in the absence of measurable inhibition of proteasome, and loss of activity of ubiquitinated enzymes such as lactate dehydrogenase and α-enolase, the protein ubiquination patterns of which is similar to those with proteasome inhibitor Bortezomib, indicating that Doxorubicin may also exert its effect by damaging proteins. [8]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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Panc02 M4e3dWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3HqXFExKM7:TR?= M2TRe|Yh\A>? NGnTbphKSzVyPUCuNFY2KM7:TR?= M2jl[Fk2OjZ3N{C=
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HOP62 MWrDfZRwfG:6aXOgRZN{[Xl? M4\OdlczKGh? M1LWRWlEPTB;MD6wNFQh|ryP M2qzeFk5PzN4NkG=
SF-295 M{C3TWN6fG:2b4jpZ{BCe3OjeR?= M2f4UFczKGh? M2DndWlEPTB;MD6wN|Uh|ryP M1fScFk5PzN4NkG=
MDA-MB-361 M2PzTGN6fG:2b4jpZ{BCe3OjeR?= NFjHdpIyKGh? MYPEUXNQ MmjyR5l1d3SxeHnjbZR6KGGpYXnud5QhVUSDLV3CMVM3OSCneIDy[ZN{cW6pIFzhZ3ohf2m2aDDJR|UxKG:oIECuNFEzKM7:TR?= NELmO3gyODN7NUS5NC=>
MDA-MB-361 M1XwW2N6fG:2b4jpZ{BCe3OjeR?= NHLSOooyKGh? NYXvXplRTE2VTx?= MXnDfZRwfG:6aXPpeJkh[WejaX7zeEBOTEFiTVKgN|YyKGW6cILld5NqdmdiY3HyZo95gXCncITp[IF{\SC|dFPQS|Ihf2m2aDDJR|UxKG:oIECuNFE5KM7:TR?= MkjoNVA{QTV2OUC=
CCRF-SB MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHLxWm01KGR? MorHSG1UVw>? Mnj5TWM2OD1yLkCxJO69VQ>? M3rBNVExPDFzNEe2
CHO-K1 M1;uV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{PV[VQh\A>? NY\NV3plTE2VTx?= Mn\lTWM2OD1yLkSg{txO MlrjNVA1OTF2N{[=
KB NGT0WpBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVG0JIQ> NITiS5JFVVOR M4q4bGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4Sg[JJ2\y2{ZYPpd5RidnRiS1K3SEB4cXSqIFnDOVAhd2ZiMD62OUDPxE1? M1i4[FExPDFzNEe2
KB M4LZ[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MY[0JIQ> MULEUXNQ MoK3RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDkdpVoNXKnc3nzeIFvfCCNQlPhcZB4cXSqIFnDOVAhd2ZiMD6wOkDPxE1? NV\iTJR{OTB2MUG0O|Y>
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HTB-54 Mmi0R5l1d3SxeHnjJGF{e2G7 MVqxNFAh|ryP MmH1TWM2OD1zLkGg{txO M33mcFExPDd4OE[x
MEL-AC NXnycFNLS3m2b4TvfIlkKEG|c3H5 NUDkOFcyOTByIN88US=> M1[0emlEPTB;MT6yJO69VQ>? MljTNVA1PzZ6NkG=
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PACA-2 M3nKOGN6fG:2b4jpZ{BCe3OjeR?= M3G5O|Yh\A>? NVLJUnpkTUR3ME2wMlAxPjNizszN NWDvPIFGOTB2OUiyNVQ>
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MKN-1 NHzOTI9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVvJR|UxRTBwNkSg{txO NIX3OmgyODZ7OESzOi=>
PC14 NWrpeJI6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVm5VoVlUUN3ME2xMlkh|ryP M{n2SFExPjl6NEO2
T24 NXLY[5hFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4XoTGlEPTB;MD62OEDPxE1? MkLJNVA3QTh2M{[=
U937 NVn1UY8{TnWwY4Tpc44hSXO|YYm= M2jvTlI1KGh? M2j5WGROW09? NX\q[mQzUW6qaXLpeJMhcW6lb4Lwc5JifGmxbjDv[kBcO0ifdHj5cYllcW6nIIfpeIghUUN3MDDv[kAxNjFizszN NWnmcXg3OTB5OEC5NVE>
H460pv8/eto MWfDfZRwfG:6aXOgRZN{[Xl? M4i3ZlExOCEQvF2= NGjrR|k1QCCq MnTZV4FtcW6n Mm\lTWM2OD1yLkCyJO69VQ>? M3raOlExPzhyOUGz
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MDA-231 MXjDfZRwfG:6aXOgRZN{[Xl? MofiNVAxKM7:TR?= MofZOFghcA>? NYHXdnVRW2GuaX7l NVXEbY9NS3m2b4TvfIlkcXS7IHHnZYlve3RiTVTBMVI{OSClZXzsJEhGWi1uRVfGVkstdXW2YX70JJA2Oylid3n0bEBKSzVyIH;mJFAvODB4IN88US=> MXOxNFc5ODlzMx?=
MDA-468 M2TId2N6fG:2b4jpZ{BCe3OjeR?= MknYNVAxKM7:TR?= M{P6V|Q5KGh? MUPTZYxqdmV? NYK1ZpNRS3m2b4TvfIlkcXS7IHHnZYlve3RiTVTBMVQ3QCClZXzsJEhGWi1uIHHtdIxq\mmnZDDFS2ZTNCCvdYThcpQheDV|KTD3bZRpKEmFNUCgc4YhOC5yMEWg{txO MlXtNVA4QDB7MUO=
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HT1080 MY\DfZRwfG:6aXOgRZN{[Xl? NX;0VGx1PzJiaB?= NWDSSmdLTE2VTx?= MXzJR|UxRTBwMEKyJO69VQ>? MlK1NVg{OjF5MUW=
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NCI-H209 NHfoTIRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVnJR|UxRTR6LkezJI5O NXjtdphkW0GQR1XS
MC-CAR MmXtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV3L[IdoUUN3ME20PE44PCCwTR?= MXjTRW5ITVJ?
NCI-H661 NGXkcpNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2fz[2lEPTB;NEmuNFMhdk1? NG\NR4xUSU6JRWK=
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NCI-H526 NVfJ[416T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NU\mNZF6UUN3ME20PU46PiCwTR?= MV3TRW5ITVJ?
Ca9-22 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3jkXGlEPTB;NUCuNFchdk1? MWnTRW5ITVJ?
SK-OV-3 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Moe5TWM2OD13MD6xNkBvVQ>? MX;TRW5ITVJ?
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CAPAN-1 NH3NO2dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnvuTWM2OD13MT63OkBvVQ>? MYDTRW5ITVJ?
NCI-H2009 Ml;GS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M17vfmlEPTB;NUGuO|Yhdk1? NU\uS3F4W0GQR1XS
NCI-H522 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmTYTWM2OD13MT65N{BvVQ>? MVrTRW5ITVJ?
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NCI-H1734 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIKzR49KSzVyPUWzJI5O MnS3V2FPT0WU
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RPMI-8402 M2frZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGrqfJNKSzVyPUWzMlg3KG6P NVr4O2hnW0GQR1XS
Calu-3 NGrTOlRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2LxSmlEPTB;NUWuPVghdk1? MmPiV2FPT0WU
COR-L279 MoPDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4PYWGlEPTB;NU[uPVUhdk1? NF34cm1USU6JRWK=
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P30-OHK MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3HS[GlEPTB;NUeuOVchdk1? NYflenVGW0GQR1XS
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KYSE-180 MoPQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{H0dmlEPTB;NUmuO|Uhdk1? MXzTRW5ITVJ?
CAMG MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoPITWM2OD13OT65OkBvVQ>? MkX2V2FPT0WU
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OVCAR-8 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX3FdmxFUUN3ME22NU46OiCwTR?= NH75boZUSU6JRWK=
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Daudi MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{flW2lEPTB;NkiuPVYhdk1? NITiSpdUSU6JRWK=
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SW962 MmO1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{X6SmlEPTB;NkmuPVUhdk1? M3zYb3NCVkeHUh?=
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DU-145 MnixS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1f6WmlEPTB;N{OuO|Mhdk1? MV3TRW5ITVJ?
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SW1573 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4n0XWlEPTB;OEKuPFEhdk1? NH7Pe|RUSU6JRWK=
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ABC-1 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlHTTWM2OD16Mz60OUBvVQ>? MUTTRW5ITVJ?
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KM-H2 M13pN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWTJR|UxRTlyLk[4JI5O MlWzV2FPT0WU
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SF268 Ml;TS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkLvTWM2OD17Mj60NkBvVQ>? NHzkdHZUSU6JRWK=
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JEG-3 MkjoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGTncnZKSzVyPUm1MlEzKG6P NXLHOZZmW0GQR1XS
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EFO-27 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MULJR|UxRTl5LkS0JI5O M4PEdXNCVkeHUh?=
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LS-441N MlyxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnXXTWM2OD17OT6wOkBvVQ>? MWXTRW5ITVJ?
G-402 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX\VdXNEUUN3ME25PU4xQSCwTR?= MnO0V2FPT0WU
MES-SA MlSxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFrscJBKSzVyPUm5MlU{KG6P Mn7jV2FPT0WU
FADU NXn3Xoh6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mli2TWM2OD1zMEKuNVghdk1? MX\TRW5ITVJ?
DJM-1 MonJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn3sTWM2OD1zMEKuNlUhdk1? NILGUXVUSU6JRWK=
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KYSE-410 NXroSGM1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVrEd4hGUUN3ME2xNFMvPDZibl2= NHzPOYlUSU6JRWK=
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AU565 MlLMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUjF[INZUUN3ME2xNFQvPTFibl2= NFHJZZZUSU6JRWK=
SF126 MnL0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHPPTm9KSzVyPUGwOE42QCCwTR?= MWfTRW5ITVJ?
MMAC-SF MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF\ubVNKSzVyPUGwOk41QCCwTR?= MU\TRW5ITVJ?
ChaGo-K-1 M{D4c2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFTqNVBKSzVyPUGwO{46PSCwTR?= MlzmV2FPT0WU
NCI-H596 MlnFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYPNVVFJUUN3ME2xNFgvODFibl2= M1e5VnNCVkeHUh?=
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HCC1569 M{OzV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVHJR|UxRTFyOT6zOEBvVQ>? MkWwV2FPT0WU
NCI-H2170 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIK1dplKSzVyPUGwPU46OiCwTR?= NGPrUHFUSU6JRWK=
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BOKU NIrOdFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEfHe5ZKSzVyPUGxNE45PyCwTR?= MW\TRW5ITVJ?
SJSA-1 NGjU[YdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2noNGlEPTB;MUGxMlA2KG6P MXvTRW5ITVJ?
SW626 NYTiNYxvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWruO4JPUUN3ME2xNVEvPzFibl2= M4XHW3NCVkeHUh?=
EHEB M1LNS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mm\TTWM2OD1zMUKuOVkhdk1? MmSwV2FPT0WU
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MZ7-mel M2nLTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUTJR|UxRTFzMj64OEBvVQ>? MoS3V2FPT0WU
SAS NVLMcpN3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYfkdm46UUN3ME2xNVQvPDVibl2= NEn0WWRUSU6JRWK=
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SW13 M4\2NGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmPNTWM2OD1zMUWuNlYhdk1? MUDTRW5ITVJ?
HCC1937 M3TYS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVrBNGZOUUN3ME2xNVUvQDRibl2= NYfWXnh2W0GQR1XS
SK-NEP-1 M37Pb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M121XGlEPTB;MUG2MlI3KG6P NV7RSpQ2W0GQR1XS
OVCAR-5 NIrFfHJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmO4TWM2OD1zMU[uPFEhdk1? NHS4dHJUSU6JRWK=
SW837 MkPpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEDvcXBKSzVyPUGxO{46PSCwTR?= NFTMWoFUSU6JRWK=
BFTC-909 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVHJR|UxRTFzOD60PEBvVQ>? MoLvV2FPT0WU
SW872 NGT2SHdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVXJR|UxRTF{MD64NUBvVQ>? MmfpV2FPT0WU
SF539 NFn0[pNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NY[4WpBTUUN3ME2xNlEvPzJibl2= NUDpd5VYW0GQR1XS
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LB647-SCLC NVXaTHZKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVj5ZplOUUN3ME2xNlMvOzVibl2= NHqybmFUSU6JRWK=
NCI-H1703 NI\hSHNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVvJR|UxRTF{Nj60NkBvVQ>? M{HqUHNCVkeHUh?=
KURAMOCHI Mo\rS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3PU[mlEPTB;MUK2Mlc3KG6P NHPiNW1USU6JRWK=
ATN-1 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlHvTWM2OD1zMkeuO{BvVQ>? NFfyclNUSU6JRWK=
SK-CO-1 NYD1PGxST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkDwTWM2OD1zMkmuNlEhdk1? NETjfWVUSU6JRWK=
RPMI-7951 M4X1PWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEPkZYlKSzVyPUGzNE4yPCCwTR?= MlPWV2FPT0WU
NCI-H1648 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlXuTWM2OD1zM{GuPVQhdk1? NFnw[opUSU6JRWK=
NCI-H64 NXTaN2lpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MULJR|UxRTF|Mj62OUBvVQ>? NF\CZ4JUSU6JRWK=
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NCI-H1770 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkTuTWM2OD1zM{OuNlEhdk1? M2TkW3NCVkeHUh?=
NB13 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVjJR|UxRTF|ND61OUBvVQ>? M3nEO3NCVkeHUh?=
NCI-H747 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXnJR|UxRTF|NT6wNkBvVQ>? MlLVV2FPT0WU
ML-2 NWeyZodwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWLGNZBQUUN3ME2xN|UvOTZibl2= M1H1UHNCVkeHUh?=
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JiyoyeP-2003 M1TKb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4ewZWlEPTB;MUO3Mlghdk1? NX3aOXYyW0GQR1XS
NMC-G1 M1XDc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX\BN|MzUUN3ME2xN|kvOTNibl2= MnXKV2FPT0WU
U-698-M MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn2xTWM2OD1zM{muOUBvVQ>? NHnqZmFUSU6JRWK=
LU-99A M4fjVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUTJR|UxRTF2Nj61OUBvVQ>? MljtV2FPT0WU
NCI-H748 NEfjfZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlL4TWM2OD1zNEeuN|Ehdk1? Mne4V2FPT0WU
SK-MEL-3 M3G2Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1v4eGlEPTB;MUS3MlYyKG6P NW[0OlJ7W0GQR1XS
SNB75 MnqzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVjJR|UxRTF2OD62OUBvVQ>? MVLTRW5ITVJ?
BB65-RCC MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmD5TWM2OD1zNEmuN|Ehdk1? NITPV2ZUSU6JRWK=
NCI-H1355 M13hT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV7xXJoyUUN3ME2xOVIvOjRibl2= NFS4ZZNUSU6JRWK=
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NCI-H2126 NVSyWmJOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn\iTWM2OD1zOUKuNkBvVQ>? MXnTRW5ITVJ?
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L-540 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGSxW|dKSzVyPUKxN{41QSCwTR?= M36zNHNCVkeHUh?=
NCI-H1882 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M17rXWlEPTB;MkG1JI5O Mnq0V2FPT0WU
A673 MnnOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mk\jTWM2OD1{MUWuO|khdk1? M4Kw[nNCVkeHUh?=
NCI-H650 MoiyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlmwTWM2OD1{MUeuOlEhdk1? MV3TRW5ITVJ?
KM12 NVzOW3VtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIDNdnRKSzVyPUKxPE4yPSCwTR?= NEfvdFJUSU6JRWK=
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SK-MEL-2 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1TvW2lEPTB;MkOwMlMhdk1? MoTqV2FPT0WU
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CAS-1 Mn72S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUDFc4pDUUN3ME2yN|gvOzFibl2= M{XzbnNCVkeHUh?=
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NCI-H520 NHrIN3NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1fC[mlEPTB;Mk[2MlYyKG6P MWjTRW5ITVJ?
HD-MY-Z M4nzOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml7BTWM2OD1{N{KuO|khdk1? NIe1NXZUSU6JRWK=
SW756 Ml\KS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH\5VGRKSzVyPUK3OE44QCCwTR?= MX7TRW5ITVJ?
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GOTO M33JT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIDEe|ZKSzVyPUK4OU42PiCwTR?= NUHNNnFLW0GQR1XS
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HOP-92 M{CzWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXW0R29rUUN3ME2yPVAvPyCwTR?= M4HJPXNCVkeHUh?=
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CAL-54 M{nuemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3u2NWlEPTB;M{CwMlc{KG6P M37WTHNCVkeHUh?=
D-263MG M{DGNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYTJR|UxRTNyNT61NkBvVQ>? M1vLO3NCVkeHUh?=
SKG-IIIa NXj0ZVZkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml\VTWM2OD1|MUGuNVQhdk1? NV\qTlE4W0GQR1XS
HAL-01 NFTNNohIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4D6b2lEPTB;M{GzMlQ1KG6P M3TGZ3NCVkeHUh?=
LAN-6 NEnzcodIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFWxdIZKSzVyPUOxOU4yOSCwTR?= MlK5V2FPT0WU
NCI-H1304 M4[5VWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml\QTWM2OD1|MkGuNVchdk1? NVPzRWZVW0GQR1XS
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L-363 NF3TcpZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXvJR|UxRTN{ND60N{BvVQ>? NWPrUoFUW0GQR1XS
EM-2 NHjpVmhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUTJR|UxRTN{NT60NUBvVQ>? M4\sfXNCVkeHUh?=
DV-90 NFXjT3RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmjRTWM2OD1|MkmuOFUhdk1? NHXOPJpUSU6JRWK=
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Raji M3TFeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MonTTWM2OD1|M{SuOlQhdk1? NX;n[o9{W0GQR1XS
RXF393 MkHMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1vOV2lEPTB;M{O1MlQ{KG6P MWLTRW5ITVJ?
EW-11 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUXJR|UxRTN2MT61OkBvVQ>? MUHTRW5ITVJ?
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LB373-MEL-D M2POfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NG\Fc4VKSzVyPUO0OE43PCCwTR?= NGiyPJFUSU6JRWK=
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GMS-10 Ml3IS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIX0VWNKSzVyPUKuN|k4OzhizszN Mn6yV2FPT0WU
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SK-N-AS NGHXbFVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV7hV|V6UUN3ME2yMlc{ODF3IN88US=> NGL3TXFUSU6JRWK=
T98G NHvEdVdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3\kPWlEPTB;Mj64NFQxOSEQvF2= NYLZV3dwW0GQR1XS
DMS-79 MoDTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVTJR|UxRTJwOUC5PFgh|ryP NXHVR5V5W0GQR1XS
SNU-475 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVPJR|UxRTJwOUK1O|Ih|ryP M1\r[nNCVkeHUh?=
COLO-792 MnvnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NILYSoJKSzVyPUOuNFI5PyEQvF2= MmizV2FPT0WU
YAPC MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlfwTWM2OD1|LkOyPFMzKM7:TR?= NX\BOVZvW0GQR1XS
UACC-812 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXm1N2JSUUN3ME2zMlM{PzN6IN88US=> MXnTRW5ITVJ?
Mewo MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlS3TWM2OD1|LkS2NFg3KM7:TR?= MUfTRW5ITVJ?
COLO-824 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFvaUZpKSzVyPUOuOVc{PzhizszN NU\I[o9qW0GQR1XS
SCC-9 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mne0TWM2OD1|LkmxN|A6KM7:TR?= NW\FUlloW0GQR1XS
CP50-MEL-B MmHrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml;oTWM2OD1|Lkm4O|A{KM7:TR?= MXXTRW5ITVJ?
NCI-H345 NV3VbJBTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHfLVmtKSzVyPUSuNFEyPzRizszN MnHoV2FPT0WU
COR-L88 M4TabWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUPYU4prUUN3ME20MlM6PThizszN MUPTRW5ITVJ?
RCM-1 MknOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmH5TWM2OD12LkS5NlczKM7:TR?= NYq5bYxJW0GQR1XS
JVM-3 M{nVOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1PYVWlEPTB;ND62NFYyQCEQvF2= M1;BVHNCVkeHUh?=
ECC4 M1;FO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUXNfWhKUUN3ME20Mlc3PTRzIN88US=> NVjlV2hEW0GQR1XS
MDA-MB-453 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn3wTWM2OD12Lki1NVM1KM7:TR?= M1[5eHNCVkeHUh?=
SK-N-FI M3LEPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1nO[mlEPTB;NT6yNlg4PyEQvF2= M3TT[XNCVkeHUh?=
D-502MG NF70eFBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2G3OGlEPTB;NT6yOFI3OiEQvF2= MXHTRW5ITVJ?
NCI-H2227 M4nCPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4HsbmlEPTB;NT6zOlI{OSEQvF2= NYO1NHZNW0GQR1XS
DMS-53 NFPqXoNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXvJR|UxRTZwMUexNFYh|ryP MnHsV2FPT0WU
LB771-HNC M{faWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV7JR|UxRTZwNE[4NFYh|ryP M3qwbHNCVkeHUh?=
BB49-HNC NUO0fW9kT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{nZN2lEPTB;Nz60NVc6OSEQvF2= NH6zT2RUSU6JRWK=
MDA-MB-415 M3n4eWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXTJR|UxRTdwNUezNFMh|ryP M3HiUHNCVkeHUh?=
TALL-1 M{\rXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkPCTWM2OD15Lkm2PFgh|ryP MkT2V2FPT0WU
HCC1395 NHHLe2tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlTITWM2OD16LkG1NVIzKM7:TR?= M{HGO3NCVkeHUh?=
EFE-184 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3K0SmlEPTB;OD6zO|IyPiEQvF2= NVTUdmY6W0GQR1XS
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TT M4XZfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH\SRWxKSzVyPUmuNFY6PjZizszN NU\4SnV{W0GQR1XS
U-2-OS MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1nUeGlEPTB;OT64NVM6OyEQvF2= MVTTRW5ITVJ?
SHP-77 NH3MPXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmPoTWM2OD17LkmwNlkh|ryP NWP6WIU5W0GQR1XS
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A498 NGHtVpNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFnqcHZKSzVyPUG4MlI6PzZizszN M3[yT3NCVkeHUh?=
LS-513 MlfES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXHJR|UxRTF6Lk[wPVMh|ryP NHP2WHpUSU6JRWK=
K052 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWDKVHc4UUN3ME2yNU41PDF6IN88US=> Ml\rV2FPT0WU
SiHa NWTRepJrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2nxfGlEPTB;MkOuN|M4OSEQvF2= Mn7lV2FPT0WU
RCC10RGB NU\IcldsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXHhRVR4UUN3ME2zNE4xPjFzIN88US=> MWTTRW5ITVJ?
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SNU-C1 NV7Ibo9OT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnrhTWM2OD1|Mj60NVc2KM7:TR?= MUHTRW5ITVJ?
C32 MmPkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX\JR|UxRTN{Lki3NkDPxE1? MWHTRW5ITVJ?
SW684 M3rqfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2P0V2lEPTB;M{SuNVU3OSEQvF2= Mm\FV2FPT0WU
SK-MEL-28 NVXWTmhQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGj5[XNKSzVyPUO1MlU{PzdizszN MXvTRW5ITVJ?
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NCI-H720 NEn3RXRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn3zTWM2OD12MT65O|I3KM7:TR?= MYnTRW5ITVJ?
P31-FUJ NGT4UoNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{\0XWlEPTB;NESuN|I5PCEQvF2= MmHqV2FPT0WU
NCI-H524 Ml3mS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NECwc4VKSzVyPUS4MlgzPDhizszN M{TGXnNCVkeHUh?=

... Click to View More Cell Line Experimental Data

In vivo In vivo, Doxorubicin in combination with adenoviral MnSOD (AdMnSOD) plus 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) has the greatest effect in decreasing the volumes of MB231 tumors and prolonging survival of mice. [1] Although its use is limited by the chronic and acute toxic side effects it produces, Doxorubicin is essential in treating breast and oesophageal carcinomas, solid tumours in childhood, osteosarcomas, Kaposi's sarcoma, soft tissue sarcomas, and Hodgkin and non-Hodgkin lymphomas. [2]

Protocol

Animal Research:[1]
+ Expand
  • Animal Models: Female athymic nude mice injected s.c. with MB231 cells
  • Formulation: Dissolved in DMSO, and diluted in saline
  • Dosages: 3 mg/kg/day
  • Administration: Delivered intratumorly
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (172.41 mM)
Water 20 mg/mL (34.48 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 579.98
Formula

C27H29NO11.HCl

CAS No. 25316-40-9
Storage powder
Synonyms NSC 123127

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03036904 Not yet recruiting Diffuse Large B-Cell Lymphoma|High Grade B-Cell Lymphoma Weill Medical College of Cornell University|Genentech, Inc.|Massachusetts General Hospital|M.D. Anderson Cancer Center February 6, 2017 Phase 1
NCT02659930 Recruiting Kaposi Sarcoma National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) January 4, 2016 Phase 1
NCT03042819 Not yet recruiting Soft Tissue Sarcoma University Health Network, Toronto|Karyopharm Therapeutics, Inc March 27, 2017 Phase 1
NCT03033914 Recruiting Hodgkin Lymphoma Memorial Sloan Kettering Cancer Center|Bristol-Myers Squibb|M.D. Anderson Cancer Center|City of Hope Medical Center January 25, 2017 Phase 1
NCT02957968 Recruiting Locally Advanced Breast Cancer|Invasive Adenocarcinoma of the Breast|Breast Cancer Stage II|Breast Cancer Stage III|Breast Cancer Stage IV Virginia Commonwealth University|Merck Sharp & Dohme Corp.|National Cancer Institute (NCI) January 24, 2017 Phase 2
NCT02377752 Active, not recruiting Neoplasm Eli Lilly and Company March 23, 2015 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    Why the IC50 of the compound varied over time in our measurement?

  • Answer:

    Sometimes the IC50 value might vary between different batch of compounds due to the slight differences in purity. If different IC50 was seen in the same batch of compound, the problem may lies in the process of dissolvement. Make sure that you see no particles and the solution is clear with your stock solution.

Topoisomerase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID