Doxorubicin (Adriamycin) HCl

Licensed by Pfizer Catalog No.S1208 Synonyms: NSC 123127

Doxorubicin (Adriamycin) HCl Chemical Structure

Molecular Weight(MW): 579.98

Doxorubicin (Adriamycin) HCl is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage and apoptosis in tumor cells.

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Cited by 33 Publications

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  • Enhancement of apoptotic response by addition of targeted agents lapatinib and sunitinib to doxorubicin in the same device reservoir at 24 hours. P values were determined using Student’s t test for all graphs.

    Sci Transl Med, 2015, 7(284): 284ra57 . Doxorubicin (Adriamycin) HCl purchased from Selleck.

    (F) Kaplan-Meier curves are shown for all groups described: no hydrogel, a hydrogel loaded with paclitaxel, or a hydrogel loaded with doxorubicin. The number of mice per group (n) and median survival (ms) are listed. The experiment was performed at least three times.

    Science, 2018, 10(433), doi: 10.1126/scitranslmed.aar1916. Doxorubicin (Adriamycin) HCl purchased from Selleck.

  • Cell viabilities with increasing concentrations of cisplatin (CP) and doxorubicin (DOXO) under normoxic and hypoxic condition for 48 hours were determined by MTT assay. IC50 values are presented as the means ?SDs (n=4) and * denotes p<0.05.

    Cancer Res 2014 74, 298-308. Doxorubicin (Adriamycin) HCl purchased from Selleck.

    Cleavage of caspase-3, -9 and the expression of PARP were measured by western blot using b-actin as a loading control.

    Cell Cycle, 2017, 16(5):468-476. Doxorubicin (Adriamycin) HCl purchased from Selleck.

  • (C) & (D) Treatments with doxorubicin, cisplatin or AZD6244 further decreased the cell viability in RH30 cloned cells in which STAT3 or GP130 was knockdown by shRNA. (*P<.05, ** P <.01, *** P <.001).

    Curr Cancer Drug Targets, 2016, 16(7):631-8. Doxorubicin (Adriamycin) HCl purchased from Selleck.

     

    Doxorubicin release of P(MAA-g-EG) and P(MAA-g-EG) containing nanoparticles in two-step pH conditions. Doxorubicinloaded P(MAA-g-EG) ,P(MAA-g-EG)-1.0NP ,P(MAA-g-EG)-2.5NP, and P(MAA-g-EG)-5.0NP crushed particles (75–150 um) were released in 1 PBS (pH 2.0) for 90 min. Then the pH was increased to 7.0 by adding 5N NaOH and release continued for 6 h.

    J Biomed Mater Res Part A 2012 10.1002/jbm.a.34064. Doxorubicin (Adriamycin) HCl purchased from Selleck.

  • Temporal uptake of free DOX or DOX-PCL63-b-PNVP90 micelles into K-562 (I) and DL (J) cells were studied by incubating in 24-well plates at a concentration of 50,000 cells per well. Representative images shown were obtained using a fluorescence microscope Eclipse 80i (Nikon, Japan) (Plan Fluor, 40X, NA 0.75 objective) equipped with blue and red filters for, Hoechst and Doxorubicin respectively (n = 3).

    PLoS One 2014 9, e94309. Doxorubicin (Adriamycin) HCl purchased from Selleck.

    PLoS One 2013 8(1), e54595. Doxorubicin (Adriamycin) HCl purchased from Selleck.

  • CON+ -positive control (with FBS as chemoattractant); CON- - negative control (without chemoattractant); DOX -doxorubicin; WZ -WZ811; PF- PF-573228; * -significant difference compared to positive control: p<0.05; ** -significant difference compared to positive control: p<0.01; # -significant difference between combination and inhibitor only: p<0.05. Cancer cell invasion was analyzed using Transwell membranes assembled in 24-well plates and covered with a layer of Matrigel. COR-L23 cells were treated for 24 h with 50 nM DOX, 10 uM WZ811, 5 uM PF-573228 and combinations of inhibitors with 50 nM DOX. Similarly, RH460 cells were treated for 24 h with 1 uM DOX, 10 uM WZ811, 2.5 uM PF-573228 and combinations of inhibitors with 1 uM DOX. Applied doses were the ones that exerted the most pronounced synergistic effect on cytotoxicity level. After the incubation period, cells that invaded through matrigel and membranes were fixed in 4% paraformaldehyde-PBS and stained with Hoechst 33342 nuclear stain. Cells were visualized and counted under a fluorescent microscope at 10x magnification.

    2014 Dr Milica Pesic from Institute for Biological Research. Doxorubicin (Adriamycin) HCl purchased from Selleck.

Purity & Quality Control

Choose Selective Topoisomerase Inhibitors

Biological Activity

Description Doxorubicin (Adriamycin) HCl is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage and apoptosis in tumor cells.
Targets
Topo II [1]
(Tumor cells)
In vitro

Doxorubicin, an antibiotic anthracycline, is commonly considered to exert its anti-tumor activity at two fundamental levels, altering DNA and producing free radicals to trigger apoptosis of cancer cells through DNA damage. Doxorubicin can block the synthesis of DNA by intercalating into the DNA strand, and inhibits DNA topoisomerase II (TOP2). Doxorubicin is most effective when cells are rapidly proliferating and expressing high levels of TOP2. Additionally, Doxorubicin can trigger apoptosis by producing ceramide (which prompts apoptosis by activating p53 or other downstream pathways such as JNK), the degradation of Akt by serine threonine proteases, the mitochondrial release of cytochrome c, increased FasL (death receptor Fas/CD95 ligand) mRNA production, and a greater production of free radicals. [2] Pre-treatment with GSNO (nitrosoglutathione) suppresses the resistance in the doxorubicin-resistant breast cancer cell line MCF7/Dx, accompanied by enhanced protein glutathionylation and accumulation of doxorubicin in the nucleus. [3] Doxorubicin induced G2/M checkpoint arrest are attributed to elevated cyclin G2 (CycG2) expression and phospho-modification of proteins in the ataxia telangiectasia mutated (ATM) and ATM and Rad3-related (ATR) signaling pathways. [5] Doxorubicin inhibits AMP-activated protein kinase (AMPK), resulting in SIRT1 dysfunction, p53 accumulation, and increased cell death in mouse embryonic fibroblasts (MEFs) and cardiomyocytes, which can be further sensitized by pre-inhibition of AMPK. [6] Doxorubicin elicits a marked heat shock response, and that either inhibition or silencing of heat shock proteins enhance the Doxorubicin apoptotic effect in neuroblastoma cells. Nanomolar Doxorubicin treatment of neuroblastoma cells causes dose-dependent over-ubiquitination of a specific set of proteins in the absence of measurable inhibition of proteasome, and loss of activity of ubiquitinated enzymes such as lactate dehydrogenase and α-enolase, the protein ubiquination patterns of which is similar to those with proteasome inhibitor Bortezomib, indicating that Doxorubicin may also exert its effect by damaging proteins. [8]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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Yoshida sarcoma MULGeY5kfGmxbjDBd5NigQ>? M2n4[WlvcGmkaYTpc44hd2ZiW{PIYXRlWiCrbnPvdpBwemG2aX;uJJdqfGhiSVO1NEBw\iByLkCyJO69VQ>? NXP1cHRrOTN{Mkm4Oi=>
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T-MT4 M4PmSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NITXc3FKTDVyPUCuNFEh|ryP M12x[FE2PDh4OEG=
SKMEL-5 MknMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIjBcVhGTDVyPUOuNkDPxE1? NEXQXHQyPjF|N{Wz
MLM NX;ZNY5qS3m2b4TvfIlkKEG|c3H5 NYn6bXJRTUR3ME2wMlE{KM7:TR?= NXvQcFRyOTh5NUO1NC=>
KBM-3 M3jmVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4r4WGlEPTB;MD6wNlYh|ryP MYWxPVk2QDd5
HT-29 M4rlT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWmwMlAyKM7:Zx?= MYO0PEBp MXvBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eR?= MkWxNlc4QDR2OR?=
EMT6 M3WwOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYSwMlUh|ryP NGLXcIYyKGh? M3v2OWlvcGmkaYTpc44hd2ZicILvcIln\XKjdHnvckBjgSCvZXHzeZJqdmdiZn;yJJN2en[rdnnu[{BnemGldHnvcpMhf2m2aDDFSFUxKG:oIECuNFAxOSEQvF2= MnLmO|Q2OjZ5NB?=
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WiDr M4L2d2N6fG:2b4jpZ{BCe3OjeR?= MX3BZ5Rqfmm2eTDh[4FqdnO2IIPlcpNqfGm4ZTDXbWRzKGOnbHyge4l1cCCLQ{WwJI9nKDBwMEWzJO69VQ>? NYXnUGJ4QDl4MEW1PC=>
MCF NV3aRmRUTnWwY4Tpc44hSXO|YYm= NFr3ZXNT\X[ncoPhcEBw\iCSLXfwMY1m\GmjdHXkJI12dHSrZIL1[{Bz\XOrc4ThcoNmKGG|c3Xzd4VlKGG|IHXubIFv[2WvZX70JIlvKGO7dH;0c5hq[2m2eTDv[kBi\HKrYX35Z4lv Mke5PFk5PDF3MR?=
C6 NFTBZpJEgXSxdH;4bYMhSXO|YYm= NFLrblQzNjVibXevcWw> MnW0O|IhcA>? MWnEUXNQ MYnDfZRwfG:6aXPpeJkh[WejaX7zeEBz[XRiQ{[gZ4VtdHNiYYSgOVAhfWdxbVygeI8hOi53IH3nM41N M3HpV|g6QDh4MES=
C6 M1;3WmZ2dmO2aX;uJGF{e2G7 NX:wTYlrOi53IH3nM41N MYi0JIg> M3fZV2ROW09? NVG0TFhTUW6qaXLpeIlwdiCxZjD0eYJ2dGmwIIDvcJlu\XKrenH0bY9v NET3W|Y5QTh6NkC0
C6 MoHJR5l1d3SxeHnjJGF{e2G7 NGLPfI8zNjVibXevcWw> MnTQOUBp NUL3WHRETE2VTx?= Mlv5R5l1d3SxeHnjbZR6KGG|c3Xzd4VlKGG|IHPlcIwhemWuZXHz[S=> M{HyUFg6QDh4MES=
SKVLB MkfER5l1d3SxeHnjJGF{e2G7 NUPZXmI2PzJiaB?= NEniXWxKSzVyPU[uOVQh|ryP NX7nS4RlQTByM{WyNC=>
MCF7/VP16 M1W4XWN6fG:2b4jpZ{BCe3OjeR?= NGDJTXA2OCEQvHevcWw> NVH5PHBMPzJiaB?= NFzFfppKSzVyPUG0MlIh|ryP MYW5NFIzPzl{
CA755 NYDnSYc{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3u3cFExKM7:TR?= MmDtOkBl MXfJR|UxRTBwMEG0JO69VQ>? Mm\qPVUzPjV5MB?=
CHO NVLvTZFuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYHVWZhxOTBizszN Ml\mOkBl NUWzXJBrUUN3ME2wMlA3KM7:TR?= Mn\2PVUzPjV5MB?=
Panc02 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGPkOIcyOCEQvF2= Ml;FOkBl MnjoTWM2OD1yLkC2OUDPxE1? NXTBTYtKQTV{NkW3NC=>
PC14 MmfGR5l1d3SxeHnjJGF{e2G7 MUS0JIQ> NVHR[2llTE2VTx?= MXjJR|UxRTBwMkmg{txO NUXpTpNCQTh{MkW0Ni=>
HOP62 MVfDfZRwfG:6aXOgRZN{[Xl? NFnsW4g4OiCq NHntXmtKSzVyPUCuNFA1KM7:TR?= NUHrb5dmQTh5M{[2NS=>
SF-295 MVLDfZRwfG:6aXOgRZN{[Xl? M4HhfVczKGh? MkP1TWM2OD1yLkCzOUDPxE1? MnnwPVg4OzZ4MR?=
MDA-MB-361 M4HHbGN6fG:2b4jpZ{BCe3OjeR?= MmXUNUBp NFOzRmZFVVOR M4jFUGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KE2GQT3NRk0{PjFiZYjwdoV{e2mwZzDMZYNbKHerdHigTWM2OCCxZjCwMlAyOiEQvF2= NV\tSmRKOTB|OUW0PVA>
MDA-MB-361 MmTiR5l1d3SxeHnjJGF{e2G7 NGfXepYyKGh? M4nmV2ROW09? MV\DfZRwfG:6aXPpeJkh[WejaX7zeEBOTEFiTVKgN|YyKGW6cILld5NqdmdiY3HyZo95gXCncITp[IF{\SC|dFPQS|Ihf2m2aDDJR|UxKG:oIECuNFE5KM7:TR?= M4TX[FExOzl3NEmw
CCRF-SB M1LoUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUK0JIQ> MnLHSG1UVw>? NEj3codKSzVyPUCuNFEh|ryP NWDnSHlIOTB2MUG0O|Y>
CHO-K1 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEHrdI41KGR? MU\EUXNQ NYG3Vpg3UUN3ME2wMlQh|ryP M4LmUFExPDFzNEe2
KB NYnWVndVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEniRZM1KGR? NF3q[Y5FVVOR MXvBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHTyeYcuemW|aYP0ZY51KEuEN1Sge4l1cCCLQ{WwJI9nKDBwNkWg{txO NET4XmEyODRzMUS3Oi=>
KB M1TyVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4j5RVQh\A>? MUfEUXNQ MWXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHTyeYcuemW|aYP0ZY51KEuEQ3HtdJdqfGhiSVO1NEBw\iByLkC2JO69VQ>? NXztTpdCOTB2MUG0O|Y>
KB NWPNZ3JrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3zDcVQh\A>? Moe1SG1UVw>? MWHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHTyeYcuemW|aYP0ZY51KEuETVTSJJdqfGhiSVO1NEBw\iByLkWg{txO NUHaV5h1OTB2MUG0O|Y>
KB M3W4[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXO0NnRNPCCm Ml6xSG1UVw>? MXXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHTyeYcuemW|aYP0ZY51KEuEVkKwR{B4cXSqIFnDOVAhd2ZiMD60JO69VQ>? MlzRNVA1OTF2N{[=
HTB-54 MXHDfZRwfG:6aXOgRZN{[Xl? MlPpNVAxKM7:TR?= M{LoRWlEPTB;MT6xJO69VQ>? MWmxNFQ4Pjh4MR?=
MEL-AC MljiR5l1d3SxeHnjJGF{e2G7 NFWyNWcyODBizszN M3vQ[GlEPTB;MT6yJO69VQ>? MUCxNFQ4Pjh4MR?=
DU145 Mm\3R5l1d3SxeHnjJGF{e2G7 MljMO|IhcA>? MlTiTWM2OD1yLkC1Nlgh|ryP MorNNVA1Pzl{OEK=
K562R NGTOV25EgXSxdH;4bYMhSXO|YYm= MlvpO|IhcA>? NYLrWHp{UUN3ME2wMlAyPTZizszN MnLRNVA1Pzl{OEK=
PACA-2 M{T6[2N6fG:2b4jpZ{BCe3OjeR?= MV[2JIQ> MmC1SWQ2OD1yLkCwOlMh|ryP MmnhNVA1QTh{MUS=
3Y1 M4GzNWN6fG:2b4jpZ{BCe3OjeR?= Mn3CO|IhcA>? MVrHTVUxRTBwMECzPEDPxE1? MYixNFY6OTdzMB?=
P388 M2j0NGN6fG:2b4jpZ{BCe3OjeR?= M3LITFczKGh? MWXHTVUxRTBwMEGyJO69VQ>? NIXZe3IyODZ7MUexNC=>
HMV-1 MnfGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWO0NYV6UUN3ME2wMlI5KM7:TR?= MVexNFY6QDR|Nh?=
MKN-1 MojiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUm3T4xvUUN3ME2wMlY1KM7:TR?= NWDwZ45DOTB4OUi0N|Y>
PC14 NULyNmRzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVfJR|UxRTFwOTFOwG0> M17OVVExPjl6NEO2
T24 MoLCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX3RSGY3UUN3ME2wMlY1KM7:TR?= M{nIUFExPjl6NEO2
U937 M2\DVGZ2dmO2aX;uJGF{e2G7 M1HIZVI1KGh? NGnQfFhFVVOR Mk\LTY5pcWKrdIOgbY5kd3Kyb4LheIlwdiCxZjDbN2hefGi7bXnkbY5mKHerdHigTWM2OCCxZjCwMlEh|ryP MVyxNFc5ODlzMR?=
H460pv8/eto NUD1cGdWS3m2b4TvfIlkKEG|c3H5 MoS2NVAxKM7:TR?= MXy0PEBp MYXTZYxqdmV? MY\JR|UxRTBwMEKg{txO NVe5S254OTB5OEC5NVM>
H460pv8 MlLrR5l1d3SxeHnjJGF{e2G7 NXWxcVFuOTByIN88US=> NYTKUnJ2PDhiaB?= NELR[FdU[WyrbnW= NXfnN|dmUUN3ME2wMlAxPCEQvF2= MUWxNFc5ODlzMx?=
MDA-231 NVvHSHZzS3m2b4TvfIlkKEG|c3H5 MVGxNFAh|ryP NY\rb2ppPDhiaB?= NIrVeZZU[WyrbnW= NFvFXJZEgXSxdH;4bYNqfHliYXfhbY5{fCCPRFGtNlMyKGOnbHygLGVTNSyHR1\SL{xufXSjboSgdFU{MSC5aYToJGlEPTBib3[gNE4xODZizszN MX:xNFc5ODlzMx?=
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NCI-H1703 MljhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX3JR|UxRTF{Nj60NkBvVQ>? MVPTRW5ITVJ?
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SNB75 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mle5TWM2OD1zNEiuOlUhdk1? MkPzV2FPT0WU
BB65-RCC Mne2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1jJfmlEPTB;MUS5MlMyKG6P NUPPUWpJW0GQR1XS
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EW-11 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoDoTWM2OD1|NEGuOVYhdk1? MUjTRW5ITVJ?
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LU-139 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH3YZo9KSzVyPUO0OE46OiCwTR?= MX7TRW5ITVJ?
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BT-20 M{TOemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYHJR|UxRTFwN{SwOlgh|ryP MVjTRW5ITVJ?
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RT4 NEn1RodIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2HXTWlEPTB;MT64OFQyPyEQvF2= MUPTRW5ITVJ?
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Hs-578-T M2rzWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoK3TWM2OD1{LkCxO|gzKM7:TR?= MnHQV2FPT0WU
HCC70 MoPQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFnISplKSzVyPUKuNFU1PjNizszN M3j0RnNCVkeHUh?=
SW1088 Mn;6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVy5VI15UUN3ME2yMlA3QTNzIN88US=> NFvieZRUSU6JRWK=
NB6 MlX0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2rCTGlEPTB;Mj6xPFUh|ryP M{CxcnNCVkeHUh?=
KU-19-19 NXK3SoYyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEezXWhKSzVyPUKuNlY6OTdizszN MYTTRW5ITVJ?
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GMS-10 M{\YcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXnJR|UxRTJwM{m3N|gh|ryP NFLVVmVUSU6JRWK=
TGBC24TKB NUXMe|dVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWfWWoVvUUN3ME2yMlQyPDJ7IN88US=> MofJV2FPT0WU
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SK-N-DZ Mlm4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV7JR|UxRTJwNU[1PVIh|ryP MWjTRW5ITVJ?
SK-N-AS MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV[0XJBHUUN3ME2yMlc{ODF3IN88US=> MUfTRW5ITVJ?
T98G NFLKdXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmfnTWM2OD1{LkiwOFAyKM7:TR?= MkfKV2FPT0WU
DMS-79 MlXNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGXlWJdKSzVyPUKuPVA6QDhizszN M2O4O3NCVkeHUh?=
SNU-475 NULBRlk1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYLDVIVPUUN3ME2yMlkzPTd{IN88US=> NYnXTYVLW0GQR1XS
COLO-792 MnTGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVrodXhCUUN3ME2zMlAzQDdizszN NUSwN|dNW0GQR1XS
YAPC MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH:yTolKSzVyPUOuN|I5OzJizszN M3\QZXNCVkeHUh?=
UACC-812 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWXJR|UxRTNwM{O3N|gh|ryP MoHVV2FPT0WU
Mewo NFntUJJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3r6WWlEPTB;Mz60OlA5PiEQvF2= NXG3fXlZW0GQR1XS
COLO-824 NHywZ5BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFLKWmFKSzVyPUOuOVc{PzhizszN NXO5OWtQW0GQR1XS
SCC-9 M1jVR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWjJR|UxRTNwOUGzNFkh|ryP Ml\hV2FPT0WU
CP50-MEL-B NGjkWHdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml\nTWM2OD1|Lkm4O|A{KM7:TR?= MmTiV2FPT0WU
NCI-H345 M2nGemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mnv1TWM2OD12LkCxNVc1KM7:TR?= M4TyVnNCVkeHUh?=
COR-L88 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWTBOIpuUUN3ME20MlM6PThizszN NYLi[GRtW0GQR1XS
RCM-1 NXXNbWdNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkTpTWM2OD12LkS5NlczKM7:TR?= NHv5eo9USU6JRWK=
JVM-3 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkfHTWM2OD12Lk[wOlE5KM7:TR?= MV7TRW5ITVJ?
ECC4 NUm1cpE{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1HoeWlEPTB;ND63OlU1OSEQvF2= M2DCTnNCVkeHUh?=
MDA-MB-453 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHTt[WFKSzVyPUSuPFUyOzRizszN Mnz4V2FPT0WU
SK-N-FI NVzaNHJZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVjJR|UxRTVwMkK4O|ch|ryP MYHTRW5ITVJ?
D-502MG MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF7XdnZKSzVyPUWuNlQzPjJizszN NULIdZZVW0GQR1XS
NCI-H2227 MlfPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoOwTWM2OD13LkO2NlMyKM7:TR?= NXu3WIlWW0GQR1XS
DMS-53 Ml3CS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV3ib|hZUUN3ME22MlE4OTB4IN88US=> MlK5V2FPT0WU
LB771-HNC M1i5N2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYLIWVVqUUN3ME22MlQ3QDB4IN88US=> MVPTRW5ITVJ?
BB49-HNC NUXPe5VOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIHHfXVKSzVyPUeuOFE4QTFizszN NHnMbZZUSU6JRWK=
MDA-MB-415 M4HUeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2jybWlEPTB;Nz61O|MxOyEQvF2= MWPTRW5ITVJ?
TALL-1 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVS0eoZYUUN3ME23Mlk3QDhizszN MnPWV2FPT0WU
HCC1395 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlzkTWM2OD16LkG1NVIzKM7:TR?= NHX1cY1USU6JRWK=
EFE-184 NYeyWWh6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFHmR4RKSzVyPUiuN|czOTZizszN NHq1XZRUSU6JRWK=
EC-GI-10 NEjnPJBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlPZTWM2OD16Lk[4Olg3KM7:TR?= NU\VOXFWW0GQR1XS
TT M4KwW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXfJR|UxRTlwME[5OlYh|ryP NELJWJdUSU6JRWK=
U-2-OS Mn;NS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUPNXXg2UUN3ME25MlgyOzl|IN88US=> MYDTRW5ITVJ?
SHP-77 NEDmPVdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVSzXopSUUN3ME25MlkxOjlizszN MX;TRW5ITVJ?
HCC1143 M1HXSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH2yTldKSzVyPUGwMlQ4QTdizszN NGjHRWpUSU6JRWK=
A498 MoPDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2rLS2lEPTB;MUiuNlk4PiEQvF2= NWHRbnYzW0GQR1XS
LS-513 NFfLbJpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1;FO2lEPTB;MUiuOlA6OyEQvF2= MmXXV2FPT0WU
K052 NYrlOGxoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmXaTWM2OD1{MT60OFE5KM7:TR?= MWfTRW5ITVJ?
SiHa NEXRTJJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGLtPVBKSzVyPUKzMlM{PzFizszN Mn3yV2FPT0WU
RCC10RGB NVK2XoJvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH3q[ZhKSzVyPUOwMlA3OTFizszN Mln4V2FPT0WU
NCI-SNU-16 MljoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3GzOWlEPTB;M{GuNVg{PiEQvF2= MXnTRW5ITVJ?
SNU-C1 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHXW[o1KSzVyPUOyMlQyPzVizszN M1TlVXNCVkeHUh?=
C32 NEfCVnpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXHINphFUUN3ME2zNk45PzJizszN Mn\6V2FPT0WU
SW684 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MW\JR|UxRTN2LkG1OlEh|ryP NIe4SJRUSU6JRWK=
SK-MEL-28 NH;wSVVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{jqWmlEPTB;M{WuOVM4PyEQvF2= MkXGV2FPT0WU
SK-MEL-1 M2\LRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV\JR|UxRTN4Lke1OFch|ryP Mm\LV2FPT0WU
NCI-H720 M2GxXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFGxUYZKSzVyPUSxMlk4OjZizszN MV7TRW5ITVJ?
P31-FUJ NIHabZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn3jTWM2OD12ND6zNlg1KM7:TR?= NVexcm5VW0GQR1XS
NCI-H524 NFXxVXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYDJR|UxRTR6LkiyOFgh|ryP MV\TRW5ITVJ?

... Click to View More Cell Line Experimental Data

In vivo In vivo, Doxorubicin in combination with adenoviral MnSOD (AdMnSOD) plus 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) has the greatest effect in decreasing the volumes of MB231 tumors and prolonging survival of mice. [1] Although its use is limited by the chronic and acute toxic side effects it produces, Doxorubicin is essential in treating breast and oesophageal carcinomas, solid tumours in childhood, osteosarcomas, Kaposi's sarcoma, soft tissue sarcomas, and Hodgkin and non-Hodgkin lymphomas. [2]

Protocol

Animal Research:[1]
+ Expand
  • Animal Models: Female athymic nude mice injected s.c. with MB231 cells
  • Formulation: Dissolved in DMSO, and diluted in saline
  • Dosages: 3 mg/kg/day
  • Administration: Delivered intratumorly
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (172.41 mM)
Water 20 mg/mL (34.48 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 579.98
Formula

C27H29NO11.HCl

CAS No. 25316-40-9
Storage powder
in solvent
Synonyms NSC 123127

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
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    Volume
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03036904 Not yet recruiting Diffuse Large B-Cell Lymphoma|High Grade B-Cell Lymphoma Weill Medical College of Cornell University|Genentech, Inc.|Massachusetts General Hospital|M.D. Anderson Cancer Center February 6, 2017 Phase 1
NCT02659930 Recruiting Kaposi Sarcoma National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) January 4, 2016 Phase 1
NCT03042819 Not yet recruiting Soft Tissue Sarcoma University Health Network, Toronto|Karyopharm Therapeutics, Inc March 27, 2017 Phase 1
NCT03033914 Recruiting Hodgkin Lymphoma Memorial Sloan Kettering Cancer Center|Bristol-Myers Squibb|M.D. Anderson Cancer Center|City of Hope Medical Center January 25, 2017 Phase 1
NCT02957968 Recruiting Locally Advanced Breast Cancer|Invasive Adenocarcinoma of the Breast|Breast Cancer Stage II|Breast Cancer Stage III|Breast Cancer Stage IV Virginia Commonwealth University|Merck Sharp & Dohme Corp.|National Cancer Institute (NCI) January 24, 2017 Phase 2
NCT02377752 Active, not recruiting Neoplasm Eli Lilly and Company March 23, 2015 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    Why the IC50 of the compound varied over time in our measurement?

  • Answer:

    Sometimes the IC50 value might vary between different batch of compounds due to the slight differences in purity. If different IC50 was seen in the same batch of compound, the problem may lies in the process of dissolvement. Make sure that you see no particles and the solution is clear with your stock solution.

Topoisomerase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID