Doxorubicin (Adriamycin)

Licensed by Pfizer Catalog No.S1208 Synonyms: NSC 123127

Doxorubicin (Adriamycin) Chemical Structure

Molecular Weight(MW): 579.98

Doxorubicin (Adriamycin) is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage and apoptosis in tumor cells.

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In DMSO USD 117 In stock
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Cited by 28 Publications

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  • Enhancement of apoptotic response by addition of targeted agents lapatinib and sunitinib to doxorubicin in the same device reservoir at 24 hours. P values were determined using Student’s t test for all graphs.

    Sci Transl Med, 2015, 7(284): 284ra57 . Doxorubicin (Adriamycin) purchased from Selleck.

    Cell viabilities with increasing concentrations of cisplatin (CP) and doxorubicin (DOXO) under normoxic and hypoxic condition for 48 hours were determined by MTT assay. IC50 values are presented as the means ?SDs (n=4) and * denotes p<0.05.

    Cancer Res 2014 74, 298-308. Doxorubicin (Adriamycin) purchased from Selleck.

  •  

    Doxorubicin release of P(MAA-g-EG) and P(MAA-g-EG) containing nanoparticles in two-step pH conditions. Doxorubicinloaded P(MAA-g-EG) ,P(MAA-g-EG)-1.0NP ,P(MAA-g-EG)-2.5NP, and P(MAA-g-EG)-5.0NP crushed particles (75–150 um) were released in 1 PBS (pH 2.0) for 90 min. Then the pH was increased to 7.0 by adding 5N NaOH and release continued for 6 h.

    J Biomed Mater Res Part A 2012 10.1002/jbm.a.34064. Doxorubicin (Adriamycin) purchased from Selleck.

    Temporal uptake of free DOX or DOX-PCL63-b-PNVP90 micelles into K-562 (I) and DL (J) cells were studied by incubating in 24-well plates at a concentration of 50,000 cells per well. Representative images shown were obtained using a fluorescence microscope Eclipse 80i (Nikon, Japan) (Plan Fluor, 40X, NA 0.75 objective) equipped with blue and red filters for, Hoechst and Doxorubicin respectively (n = 3).

    PLoS One 2014 9, e94309. Doxorubicin (Adriamycin) purchased from Selleck.

  • PLoS One 2013 8(1), e54595. Doxorubicin (Adriamycin) purchased from Selleck.

    CON+ -positive control (with FBS as chemoattractant); CON- - negative control (without chemoattractant); DOX -doxorubicin; WZ -WZ811; PF- PF-573228; * -significant difference compared to positive control: p<0.05; ** -significant difference compared to positive control: p<0.01; # -significant difference between combination and inhibitor only: p<0.05. Cancer cell invasion was analyzed using Transwell membranes assembled in 24-well plates and covered with a layer of Matrigel. COR-L23 cells were treated for 24 h with 50 nM DOX, 10 uM WZ811, 5 uM PF-573228 and combinations of inhibitors with 50 nM DOX. Similarly, RH460 cells were treated for 24 h with 1 uM DOX, 10 uM WZ811, 2.5 uM PF-573228 and combinations of inhibitors with 1 uM DOX. Applied doses were the ones that exerted the most pronounced synergistic effect on cytotoxicity level. After the incubation period, cells that invaded through matrigel and membranes were fixed in 4% paraformaldehyde-PBS and stained with Hoechst 33342 nuclear stain. Cells were visualized and counted under a fluorescent microscope at 10x magnification.

    2014 Dr Milica Pesic from Institute for Biological Research. Doxorubicin (Adriamycin) purchased from Selleck.

Purity & Quality Control

Choose Selective Topoisomerase Inhibitors

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Notes:

2. For more details, such as half maximal inhibitory concentrations (IC50s) and working concentrations of each inhibitor, please click on the link of the inhibitor of interest.
3. "+" indicates inhibitory effect. Increased inhibition is marked by a higher "+" designation.
4. Orange "√" refers to compounds which do inhibitory effects on the related isoform, but without specific value.

Biological Activity

Description Doxorubicin (Adriamycin) is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage and apoptosis in tumor cells.
Targets
Topo II [1]
(Tumor cells)
In vitro

Doxorubicin, an antibiotic anthracycline, is commonly considered to exert its anti-tumor activity at two fundamental levels, altering DNA and producing free radicals to trigger apoptosis of cancer cells through DNA damage. Doxorubicin can block the synthesis of DNA by intercalating into the DNA strand, and inhibits DNA topoisomerase II (TOP2). Doxorubicin is most effective when cells are rapidly proliferating and expressing high levels of TOP2. Additionally, Doxorubicin can trigger apoptosis by producing ceramide (which prompts apoptosis by activating p53 or other downstream pathways such as JNK), the degradation of Akt by serine threonine proteases, the mitochondrial release of cytochrome c, increased FasL (death receptor Fas/CD95 ligand) mRNA production, and a greater production of free radicals. [2] Pre-treatment with GSNO (nitrosoglutathione) suppresses the resistance in the doxorubicin-resistant breast cancer cell line MCF7/Dx, accompanied by enhanced protein glutathionylation and accumulation of doxorubicin in the nucleus. [3] Doxorubicin induced G2/M checkpoint arrest are attributed to elevated cyclin G2 (CycG2) expression and phospho-modification of proteins in the ataxia telangiectasia mutated (ATM) and ATM and Rad3-related (ATR) signaling pathways. [5] Doxorubicin inhibits AMP-activated protein kinase (AMPK), resulting in SIRT1 dysfunction, p53 accumulation, and increased cell death in mouse embryonic fibroblasts (MEFs) and cardiomyocytes, which can be further sensitized by pre-inhibition of AMPK. [6] Doxorubicin elicits a marked heat shock response, and that either inhibition or silencing of heat shock proteins enhance the Doxorubicin apoptotic effect in neuroblastoma cells. Nanomolar Doxorubicin treatment of neuroblastoma cells causes dose-dependent over-ubiquitination of a specific set of proteins in the absence of measurable inhibition of proteasome, and loss of activity of ubiquitinated enzymes such as lactate dehydrogenase and α-enolase, the protein ubiquination patterns of which is similar to those with proteasome inhibitor Bortezomib, indicating that Doxorubicin may also exert its effect by damaging proteins. [8]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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MCF NYTZNo5XTnWwY4Tpc44hSXO|YYm= MmDwVoV3\XK|YXygc4YhWC2pcD3t[YRq[XSnZDDteYx1cWS{dXegdoV{cXO2YX7j[UBie3Onc4Pl[EBieyCnbnjhcoNmdWWwdDDpckBkgXSxdH;4bYNqfHlib3[gZYRzcWGveXPpci=> NG\TdFE5QTh2MUWx
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CHO Mmm1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYWxNEDPxE1? NXPVRpF1PiCm M1;5cmlEPTB;MD6wOkDPxE1? NGTwZZk6PTJ4NUew
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PC14 MnnKR5l1d3SxeHnjJGF{e2G7 NHP1dVY1KGR? MYPEUXNQ NWTGcIhEUUN3ME2wMlI6KM7:TR?= MUW5PFIzPTR{
HOP62 NUm3XW5pS3m2b4TvfIlkKEG|c3H5 M3;xcVczKGh? MlXDTWM2OD1yLkCwOEDPxE1? MkPLPVg4OzZ4MR?=
SF-295 NUna[Yh7S3m2b4TvfIlkKEG|c3H5 NH7sN3U4OiCq M{DGSGlEPTB;MD6wN|Uh|ryP MVO5PFc{PjZz
MDA-MB-361 MomwR5l1d3SxeHnjJGF{e2G7 NIHrZ4QyKGh? NGLFNoJFVVOR NHOyZlZEgXSxdH;4bYNqfHliYXfhbY5{fCCPRFGtUWIuOzZzIHX4dJJme3OrbnegUIFkYiC5aYToJGlEPTBib3[gNE4xOTJizszN M4nRNlExOzl3NEmw
MDA-MB-361 NFOyZ5hEgXSxdH;4bYMhSXO|YYm= NGThUoEyKGh? M33pbGROW09? MV\DfZRwfG:6aXPpeJkh[WejaX7zeEBOTEFiTVKgN|YyKGW6cILld5NqdmdiY3HyZo95gXCncITp[IF{\SC|dFPQS|Ihf2m2aDDJR|UxKG:oIECuNFE5KM7:TR?= M4PORlExOzl3NEmw
CCRF-SB NFLndo1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXq0JIQ> MXPEUXNQ NXTpZXMxUUN3ME2wMlAyKM7:TR?= NHjWRo4yODRzMUS3Oi=>
CHO-K1 M3nkcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVvCXGZGPCCm MVvEUXNQ M2LhUGlEPTB;MD60JO69VQ>? MXyxNFQyOTR5Nh?=
KB NHyw[lRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXW0JIQ> MUnEUXNQ NGjWOotCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIRzfWdvcnXzbZN1[W62IFvCO2Qhf2m2aDDJR|UxKG:oIECuOlUh|ryP MYSxNFQyOTR5Nh?=
KB NYPKc|Z3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVu0JIQ> NH\udnZFVVOR MlLQRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDkdpVoNXKnc3nzeIFvfCCNQlPhcZB4cXSqIFnDOVAhd2ZiMD6wOkDPxE1? MoHlNVA1OTF2N{[=
KB M1LNc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1O4OlQh\A>? NFzldIJFVVOR NIjFSpRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIRzfWdvcnXzbZN1[W62IFvCUWRTKHerdHigTWM2OCCxZjCwMlUh|ryP MmPzNVA1OTF2N{[=
KB NFPZW41Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYH6fpZoPCCm M1i5fGROW09? M4G5d2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4Sg[JJ2\y2{ZYPpd5RidnRiS1LWNlBEKHerdHigTWM2OCCxZjCwMlQh|ryP M1HRWFExPDFzNEe2
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MEL-AC NYPSOFVNS3m2b4TvfIlkKEG|c3H5 M4XkRVExOCEQvF2= M3fTPWlEPTB;MT6yJO69VQ>? M3LxXFExPDd4OE[x
DU145 NEPPXlNEgXSxdH;4bYMhSXO|YYm= NIfrNJQ4OiCq MkTkTWM2OD1yLkC1Nlgh|ryP M3TWW|ExPDd7Mkiy
K562R MV\DfZRwfG:6aXOgRZN{[Xl? M3HWU|czKGh? NH73N25KSzVyPUCuNFE2PiEQvF2= NV7OUodqOTB2N{myPFI>
PACA-2 Mn7oR5l1d3SxeHnjJGF{e2G7 MoPSOkBl NVnNNYhMTUR3ME2wMlAxPjNizszN NE\ES5kyODR7OEKxOC=>
3Y1 M4e5RmN6fG:2b4jpZ{BCe3OjeR?= MXm3NkBp M3jQSWdKPTB;MD6wNFM5KM7:TR?= M3zGdFExPjlzN{Gw
P388 NUPi[nlNS3m2b4TvfIlkKEG|c3H5 MXS3NkBp MXXHTVUxRTBwMEGyJO69VQ>? Mn\BNVA3QTF5MUC=
HMV-1 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{W5WGlEPTB;MD6yPEDPxE1? M2fkR|ExPjl6NEO2
MKN-1 MoWwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVrTeJJQUUN3ME2wMlY1KM7:TR?= MnjINVA3QTh2M{[=
PC14 M{n6[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnHUTWM2OD1zLkmg{txO NUS1NlZuOTB4OUi0N|Y>
T24 MlnaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXO0VndyUUN3ME2wMlY1KM7:TR?= NFXzcZEyODZ7OESzOi=>
U937 MnLUSpVv[3Srb36gRZN{[Xl? M2j5WlI1KGh? MWXEUXNQ NWPse2p5UW6qaXLpeJMhcW6lb4Lwc5JifGmxbjDv[kBcO0ifdHj5cYllcW6nIIfpeIghUUN3MDDv[kAxNjFizszN M4PJVFExPzhyOUGx
H460pv8/eto MU\DfZRwfG:6aXOgRZN{[Xl? NV\6THFTOTByIN88US=> MojmOFghcA>? M1fSO3NidGmwZR?= M1\QcGlEPTB;MD6wNkDPxE1? NHX3ZZAyODd6MEmxNy=>
H460pv8 M1LF[2N6fG:2b4jpZ{BCe3OjeR?= NInacngyODBizszN M{fRWlQ5KGh? MUTTZYxqdmV? MonUTWM2OD1yLkCwOEDPxE1? NXrDWW1OOTB5OEC5NVM>
MDA-231 NWX6O2JjS3m2b4TvfIlkKEG|c3H5 NFrXRVEyODBizszN NUjSe45JPDhiaB?= Mk\RV4FtcW6n Mn\yR5l1d3SxeHnjbZR6KGGpYXnud5QhVUSDLUKzNUBk\WyuIDjFVk0tTUeIUjuscZV1[W62IIC1N{khf2m2aDDJR|UxKG:oIECuNFA3KM7:TR?= NW[xUG9UOTB5OEC5NVM>
MDA-468 NVOze5U4S3m2b4TvfIlkKEG|c3H5 MmXkNVAxKM7:TR?= NX3FcIJoPDhiaB?= M1jubXNidGmwZR?= M1\ybmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KE2GQT20Olgh[2WubDCoSXIuNCCjbYDsbYZq\WRiRVfGVkwhdXW2YX70JJA2Oylid3n0bEBKSzVyIH;mJFAvODB3IN88US=> M1fK[|ExPzhyOUGz
NCI-H226 Mn;WR5l1d3SxeHnjJGF{e2G7 MlvpNVAxKM7:TR?= NHvMUGc1QCCq NXzQfFRFW2GuaX7l MmmyR5l1d3SxeHnjbZR6KGGpYXnud5QhVkOLLViyNlYh[2WubDCocZV1[W62IIC1N{khf2m2aDDJR|UxKG:oIECuNFA5KM7:TR?= MmPENVA4QDB7MUO=
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MDA-MB-231 NX7leYFOTnWwY4Tpc44hSXO|YYm= MorBOUDPxE1? M4DrW|I1KGh? M3jOW2NmdGy3bHHyJJVxfGGtZR?= MUeyN|k4OzJzMx?=
MDA-MB-231 MVzGeY5kfGmxbjDBd5NigQ>? NXvEb|lGPSEQvF2= NYq1d3Y6OjRiaB?= MnnMRY51cW:6aXThcpQh[WO2aY\peJkh[XO|ZYPz[YQh[XNiaX70doFk\WyudXzhdkBu[Wyxbons[IlidGSnaInk[UBt\X[nbB?= Mor3NlM6PzN{MUO=
MDA-MB-231 NH25bIZHfW6ldHnvckBCe3OjeR?= NGOwT|g2KM7:TR?= NXO5e21kOjRiaB?= MlzZRY51cW:6aXThcpQh[WO2aY\peJkh[XO|ZYPz[YQh[XNiaX70doFucXSxY3jvcoRzcWGuIGLPV{Bt\X[nbB?= NYDSV5VvOjN7N{OyNVM>
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MDA-MB-435 M{PWbGN6fG:2b4jpZ{BCe3OjeR?= MnLHNVAxKM7:TR?= MmDPOFghcA>? NEXJ[mhKSzVyPUCuNVUh|ryP NUDVNlltOjRyMki0OFY>
AS49-RAW NFT5WG1EgXSxdH;4bYMhSXO|YYm= Mnm0NVAxKM7:TR?= MnmwOFghcA>? M3ntO2lEPTB;M{KuO|gh|ryP M1mwU|I1ODd5NUK4
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LNcaP MXzGeY5kfGmxbjDBd5NigQ>? NIXxTlU{KM7:TR?= M1q1TlI1KGh? MoLFTY5kemWjc3Wgc4Yh[2ynYY\l[EBk[XOyYYPlMVkhdGW4ZXy= NGf2d5IzPDB7NUC4PS=>
LNcaP M1vIO2Z2dmO2aX;uJGF{e2G7 MV[zJO69VQ>? NF3IOVEzPCCq M4GxT3VxNXKnZ4XsZZRqd25ib3[gdFU{KGW6cILld5Nqd25? M1jyTlI1ODl3MEi5
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NCI-H1437 M3;Vemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3SyNWlEPTB;NkSuOlQhdk1? MW\TRW5ITVJ?
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RPMI-6666 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MW\JR|UxRTZ3Lkm5JI5O M3LqcnNCVkeHUh?=
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GT3TKB NF21T2tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV33eFdSUUN3ME22O{4yQSCwTR?= MnHSV2FPT0WU
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SW962 M{Gye2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXPJR|UxRTZ7Lkm1JI5O MoDpV2FPT0WU
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DU-145 M2i1Z2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV\JR|UxRTd|LkezJI5O M17m[XNCVkeHUh?=
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HCC1806 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX;JR|UxRTd2Lkm2JI5O MorrV2FPT0WU
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NH-12 NXP6SHpCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NITyNIdKSzVyPUe2JI5O NVfvS5NmW0GQR1XS
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CMK MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHjaSldKSzVyPUe3MlQzKG6P NGmzNpVUSU6JRWK=
LB996-RCC MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGjFUo9KSzVyPUe4MlQ2KG6P NYXFOXduW0GQR1XS
NCI-H1650 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVPJR|UxRTd6Lk[yJI5O MlP6V2FPT0WU
DMS-273 NFLjeldIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVzJR|UxRTd6Lkmgcm0> M3z4cXNCVkeHUh?=
NOS-1 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXzBT4xIUUN3ME23PU4yPCCwTR?= M3rDcXNCVkeHUh?=
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A172 MmfXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV7JR|UxRThyLkW1JI5O NXjyTWZSW0GQR1XS
TE-12 M2DYUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYnvdHE5UUN3ME24NU4xPiCwTR?= NHvrPGRUSU6JRWK=
MKN45 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVfJR|UxRThzLkWxJI5O M4C0N3NCVkeHUh?=
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SW1573 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4e0PGlEPTB;OEKuPFEhdk1? MWjTRW5ITVJ?
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ABC-1 NVLqTFBbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHXxOndKSzVyPUizMlQ2KG6P MYnTRW5ITVJ?
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PANC-10-05 NWXLR|NkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUfJR|UxRTh6LkS1JI5O MnnsV2FPT0WU
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TE-441-T NHTpU5JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlHOTWM2OD16OT64NkBvVQ>? MY\TRW5ITVJ?
KM-H2 NUfBXo8{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVfJR|UxRTlyLk[4JI5O M1z0PXNCVkeHUh?=
SH-4 NFvKR5lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4TxdGlEPTB;OUKuNVEhdk1? MmTqV2FPT0WU
SF268 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MU\JR|UxRTl{LkSyJI5O M121XXNCVkeHUh?=
VMRC-RCZ MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXLJR|UxRTl|LkK2JI5O MorOV2FPT0WU
GI-ME-N MkizS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYfxb|BsUUN3ME25N{4{OyCwTR?= M4LvS3NCVkeHUh?=
JEG-3 NUTwfmVDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF3GRphKSzVyPUm1MlEzKG6P MonoV2FPT0WU
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K5 NWH1d5VRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mnj5TWM2OD17Nj65JI5O NE\j[FdUSU6JRWK=
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SW1783 NH3Kb5hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MY\JR|UxRTl5Lkm5JI5O MlvCV2FPT0WU
CAL-39 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3fGfWlEPTB;OUiuNlQhdk1? NXS1SpZYW0GQR1XS
LS-441N M1juVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYfpTW9[UUN3ME25PU4xPiCwTR?= M33uS3NCVkeHUh?=
G-402 NVXSW5ZlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF3OVVlKSzVyPUm5MlA6KG6P M1Ph[3NCVkeHUh?=
MES-SA NVz3d2NVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWTveYtKUUN3ME25PU42OyCwTR?= NHXXbmRUSU6JRWK=
FADU NVjj[pVtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mm\aTWM2OD1zMEKuNVghdk1? Mof4V2FPT0WU
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SU-DHL-1 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MofrTWM2OD1zMEKuOUBvVQ>? MXfTRW5ITVJ?
KYSE-410 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MW\JR|UxRTFyMz60OkBvVQ>? NUnWZoUzW0GQR1XS
NCI-H1618 NF:3RZZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWnjVXBCUUN3ME2xNFQvOzVibl2= NFWzTYZUSU6JRWK=
AU565 M1\rXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NETvdYhKSzVyPUGwOE42OSCwTR?= MYHTRW5ITVJ?
SF126 NH:3WVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHnXRpRKSzVyPUGwOE42QCCwTR?= MlnJV2FPT0WU
MMAC-SF M{DIOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M17hSGlEPTB;MUC2MlQ5KG6P MmnqV2FPT0WU
ChaGo-K-1 M2myfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEHBfI1KSzVyPUGwO{46PSCwTR?= NVfnS2tOW0GQR1XS
NCI-H596 MmfSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{fQeGlEPTB;MUC4MlAyKG6P MYfTRW5ITVJ?
LXF-289 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmPuTWM2OD1zMEmuNlQhdk1? MYjTRW5ITVJ?
HCC1569 M3\sSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3XxNmlEPTB;MUC5MlM1KG6P M3rUWnNCVkeHUh?=
NCI-H2170 NHPJWYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWnmTHhtUUN3ME2xNFkvQTJibl2= MnP6V2FPT0WU
ARH-77 NGPoWXdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1\Xd2lEPTB;MUC5Mlk6KG6P MXHTRW5ITVJ?
BOKU MknYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH;0bmJKSzVyPUGxNE45PyCwTR?= M3jDRnNCVkeHUh?=
SJSA-1 NYC5Z|l{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M13zd2lEPTB;MUGxMlA2KG6P NHLuNnBUSU6JRWK=
SW626 NFvjPWtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWnBbYRuUUN3ME2xNVEvPzFibl2= NH\2[3BUSU6JRWK=
EHEB NYrzS49yT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEPYeHBKSzVyPUGxNk42QSCwTR?= NX:ySXo5W0GQR1XS
TE-10 MnrYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{H4dmlEPTB;MUGyMlY5KG6P NIHndFFUSU6JRWK=
MZ7-mel NITHWIFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUfiU|AyUUN3ME2xNVIvQDRibl2= NFfFdZlUSU6JRWK=
SAS NEe3b2dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHfJSZBKSzVyPUGxOE41PSCwTR?= NGrOOmJUSU6JRWK=
TK10 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHK5VZhKSzVyPUGxOE43KG6P NVvxU3I5W0GQR1XS
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HCC1937 MoLoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkjiTWM2OD1zMUWuPFQhdk1? MULTRW5ITVJ?
SK-NEP-1 MnnIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2X1SWlEPTB;MUG2MlI3KG6P M2PYU3NCVkeHUh?=
OVCAR-5 MoLBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWjJR|UxRTFzNj64NUBvVQ>? NUTXSJJ3W0GQR1XS
SW837 NHK3[5ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUPCbmtSUUN3ME2xNVcvQTVibl2= NYDsSWpkW0GQR1XS
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MLMA NH7DUYpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVj5[GVlUUN3ME2xNlIvODhibl2= M3nIS3NCVkeHUh?=
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LB647-SCLC M4LEOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NI\MeItKSzVyPUGyN{4{PSCwTR?= NU\VSmo1W0GQR1XS
NCI-H1703 NIjTSJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlruTWM2OD1zMk[uOFIhdk1? NXm1UHc2W0GQR1XS
KURAMOCHI MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2q1e2lEPTB;MUK2Mlc3KG6P NWLXN2NEW0GQR1XS
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RPMI-7951 M{DNd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2\MXWlEPTB;MUOwMlE1KG6P NHrsOnRUSU6JRWK=
NCI-H1648 NHvtelRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUflVWxDUUN3ME2xN|EvQTRibl2= MoHvV2FPT0WU
NCI-H64 M1SycWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1fBPWlEPTB;MUOyMlY2KG6P MV;TRW5ITVJ?
HT-3 M2P2R2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mom0TWM2OD1zM{OuNFQhdk1? M3v2fXNCVkeHUh?=
NCI-H1770 MlvmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mo\wTWM2OD1zM{OuNlEhdk1? Mnj2V2FPT0WU
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NCI-H747 NYrscYxCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX7JR|UxRTF|NT6wNkBvVQ>? NFXvc2VUSU6JRWK=
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U-698-M NWPBV|BWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M37pSmlEPTB;MUO5MlUhdk1? NF7NRXNUSU6JRWK=
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NCI-H748 NXW5cZpjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml:wTWM2OD1zNEeuN|Ehdk1? MnjlV2FPT0WU
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SNB75 MmLIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXrJR|UxRTF2OD62OUBvVQ>? M3f1UHNCVkeHUh?=
BB65-RCC MoPxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV72dWZoUUN3ME2xOFkvOzFibl2= Mm\tV2FPT0WU
NCI-H1355 MmTmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWrJR|UxRTF3Mj6yOEBvVQ>? NWHrd|hFW0GQR1XS
SCC-25 NGO3WmdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUjJR|UxRTF3Mz63N{BvVQ>? NHrzOHVUSU6JRWK=
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NCI-H1573 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGryNIFKSzVyPUGuO|E4OzNizszN MlzuV2FPT0WU
BT-20 NW[yboRIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MU\JR|UxRTFwN{SwOlgh|ryP MV;TRW5ITVJ?
NCI-H28 MnPsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEi0VnFKSzVyPUGuO|QxPzhizszN NVS2N2FlW0GQR1XS
CaR-1 MlXXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUnJR|UxRTFwN{izNlQh|ryP NEPJb5NUSU6JRWK=
LN-405 MofIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1ftXWlEPTB;MT64NFE1OyEQvF2= Mke0V2FPT0WU
RT4 M3L0fGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV3uWlZHUUN3ME2xMlg1PDF5IN88US=> NFjwS|hUSU6JRWK=
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SW1463 NWDQUIp7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3\jfmlEPTB;MT65OVcxOSEQvF2= MlnmV2FPT0WU
SCLC-21H M4jlcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWXYSGVwUUN3ME2yMlAxPjJ2IN88US=> M{ntd3NCVkeHUh?=
CP66-MEL M2PMPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn3BTWM2OD1{LkCxO|Y4KM7:TR?= NYDsU5NkW0GQR1XS
Hs-578-T NF:zXFJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVzJR|UxRTJwMEG3PFIh|ryP MX;TRW5ITVJ?
HCC70 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2rUWGlEPTB;Mj6wOVQ3OyEQvF2= M{XsNnNCVkeHUh?=
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NB6 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIfw[4tKSzVyPUKuNVg2KM7:TR?= MlLtV2FPT0WU
KU-19-19 NX3kRmh1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHnjUXdKSzVyPUKuNlY6OTdizszN MVrTRW5ITVJ?
A704 MkDPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlnwTWM2OD1{LkK5NVM2KM7:TR?= MXrTRW5ITVJ?
EVSA-T MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFfQTG5KSzVyPUKuN|AxQDlizszN MojJV2FPT0WU
HCT-15 MkPxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVTpOJVjUUN3ME2yMlM2PDZ4IN88US=> MXHTRW5ITVJ?
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T98G NHHVZmhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWrJR|UxRTJwOEC0NFEh|ryP NFK3cXpUSU6JRWK=
DMS-79 MkjiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYnhRYh6UUN3ME2yMlkxQTh6IN88US=> MWjTRW5ITVJ?
SNU-475 NYTMdGh2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmrPTWM2OD1{LkmyOVczKM7:TR?= NWXNcINkW0GQR1XS
COLO-792 NI\jR3RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NITqTVJKSzVyPUOuNFI5PyEQvF2= M4fKZXNCVkeHUh?=
YAPC MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUTvRWljUUN3ME2zMlMzQDN{IN88US=> Mk\lV2FPT0WU
UACC-812 NIrBSm5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWHJR|UxRTNwM{O3N|gh|ryP NETGXHlUSU6JRWK=
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COLO-824 NUKzNpNvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUnzPY9YUUN3ME2zMlU4Ozd6IN88US=> M1:2d3NCVkeHUh?=
SCC-9 NFTnZXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{m5OGlEPTB;Mz65NVMxQSEQvF2= MoD1V2FPT0WU
CP50-MEL-B M3PsTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHjRVmNKSzVyPUOuPVg4ODNizszN M37VO3NCVkeHUh?=
NCI-H345 M4LtU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFe2eGxKSzVyPUSuNFEyPzRizszN NX3X[JNQW0GQR1XS
COR-L88 NXPhZ2RZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYTJR|UxRTRwM{m1PEDPxE1? NIjheFJUSU6JRWK=
RCM-1 NHPKUnlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3S4b2lEPTB;ND60PVI4OiEQvF2= NWrvTIVnW0GQR1XS
JVM-3 MkjmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWqzNG15UUN3ME20MlYxPjF6IN88US=> Mmj4V2FPT0WU
ECC4 MnK1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX7JR|UxRTRwN{[1OFEh|ryP M{nGPHNCVkeHUh?=
MDA-MB-453 NFPDTJBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX;JR|UxRTRwOEWxN|Qh|ryP NYDicIpTW0GQR1XS
SK-N-FI NGfjRnNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVfJR|UxRTVwMkK4O|ch|ryP NEnsR25USU6JRWK=
D-502MG M3Sy[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmfhTWM2OD13LkK0NlYzKM7:TR?= MXPTRW5ITVJ?
NCI-H2227 Mn;RS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFLhfGlKSzVyPUWuN|YzOzFizszN M3ju[3NCVkeHUh?=
DMS-53 NVPmc|ZqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYDJR|UxRTZwMUexNFYh|ryP NVXjfYFsW0GQR1XS
LB771-HNC MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEDiTGJKSzVyPU[uOFY5ODZizszN MUXTRW5ITVJ?
BB49-HNC MkDIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mk\TTWM2OD15LkSxO|kyKM7:TR?= NX\vdYRKW0GQR1XS
MDA-MB-415 M2n3fmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml7ITWM2OD15LkW3N|A{KM7:TR?= MX3TRW5ITVJ?
TALL-1 NFjqdFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXX0cJpTUUN3ME23Mlk3QDhizszN MYLTRW5ITVJ?
HCC1395 NHPZ[FlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MofvTWM2OD16LkG1NVIzKM7:TR?= M3\zd3NCVkeHUh?=
EFE-184 MoPWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH\ON2JKSzVyPUiuN|czOTZizszN NVvFWIhtW0GQR1XS
EC-GI-10 MlXiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlnLTWM2OD16Lk[4Olg3KM7:TR?= MUfTRW5ITVJ?
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A498 NVTXOJFDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NI\PeFFKSzVyPUG4MlI6PzZizszN NVfYV4VtW0GQR1XS
LS-513 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUXJR|UxRTF6Lk[wPVMh|ryP MXfTRW5ITVJ?
K052 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkfXTWM2OD1{MT60OFE5KM7:TR?= NIK2Vo9USU6JRWK=
SiHa MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV7JR|UxRTJ|LkOzO|Eh|ryP M1zSNnNCVkeHUh?=
RCC10RGB MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVT3epBKUUN3ME2zNE4xPjFzIN88US=> M{m3SXNCVkeHUh?=
NCI-SNU-16 NGDzSFJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4fKWWlEPTB;M{GuNVg{PiEQvF2= M3LEUXNCVkeHUh?=
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C32 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVnJR|UxRTN{Lki3NkDPxE1? NF33VndUSU6JRWK=
SW684 NFnXVmdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF;YXHFKSzVyPUO0MlE2PjFizszN MojOV2FPT0WU
SK-MEL-28 NHzScWFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M13we2lEPTB;M{WuOVM4PyEQvF2= M2K3ZXNCVkeHUh?=
SK-MEL-1 MlPYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4LuWGlEPTB;M{[uO|U1PyEQvF2= Mm\LV2FPT0WU
NCI-H720 MkLoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVnITHV3UUN3ME20NU46PzJ4IN88US=> NHSwXGRUSU6JRWK=
P31-FUJ M1zm[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3HKeWlEPTB;NESuN|I5PCEQvF2= NUTVe|JzW0GQR1XS
NCI-H524 NXnNPY9iT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGXQNGJKSzVyPUS4MlgzPDhizszN M2noeXNCVkeHUh?=

... Click to View More Cell Line Experimental Data

In vivo In vivo, Doxorubicin in combination with adenoviral MnSOD (AdMnSOD) plus 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) has the greatest effect in decreasing the volumes of MB231 tumors and prolonging survival of mice. [1] Although its use is limited by the chronic and acute toxic side effects it produces, Doxorubicin is essential in treating breast and oesophageal carcinomas, solid tumours in childhood, osteosarcomas, Kaposi's sarcoma, soft tissue sarcomas, and Hodgkin and non-Hodgkin lymphomas. [2]

Protocol

Animal Research:[1]
+ Expand
  • Animal Models: Female athymic nude mice injected s.c. with MB231 cells
  • Formulation: Dissolved in DMSO, and diluted in saline
  • Dosages: 3 mg/kg/day
  • Administration: Delivered intratumorly
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (172.41 mM) warming
Water 20 mg/mL (34.48 mM)
Ethanol <1 mg/mL
In vivo

* 1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 579.98
Formula

C27H29NO11.HCl

CAS No. 25316-40-9
Storage powder
in solvent
Synonyms NSC 123127

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03036904 Not yet recruiting Diffuse Large B-Cell Lymphoma|High Grade B-Cell Lymphoma Weill Medical College of Cornell University|Genentech, Inc.|Massachusetts General Hospital|M.D. Anderson Cancer Center February 6, 2017 Phase 1
NCT02659930 Recruiting Kaposi Sarcoma National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) January 4, 2016 Phase 1
NCT03042819 Not yet recruiting Soft Tissue Sarcoma University Health Network, Toronto|Karyopharm Therapeutics, Inc March 27, 2017 Phase 1
NCT03033914 Recruiting Hodgkin Lymphoma Memorial Sloan Kettering Cancer Center|Bristol-Myers Squibb|M.D. Anderson Cancer Center|City of Hope Medical Center January 25, 2017 Phase 1
NCT02957968 Recruiting Locally Advanced Breast Cancer|Invasive Adenocarcinoma of the Breast|Breast Cancer Stage II|Breast Cancer Stage III|Breast Cancer Stage IV Virginia Commonwealth University|Merck Sharp & Dohme Corp.|National Cancer Institute (NCI) January 24, 2017 Phase 2
NCT02377752 Active, not recruiting Neoplasm Eli Lilly and Company March 23, 2015 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Topoisomerase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID