Doxorubicin (Adriamycin) HCl

Licensed by Pfizer Catalog No.S1208 Synonyms: NSC 123127

Doxorubicin (Adriamycin) HCl Chemical Structure

Molecular Weight(MW): 579.98

Doxorubicin (Adriamycin) HCl is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage and apoptosis in tumor cells.

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Cited by 32 Publications

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  • Enhancement of apoptotic response by addition of targeted agents lapatinib and sunitinib to doxorubicin in the same device reservoir at 24 hours. P values were determined using Student’s t test for all graphs.

    Sci Transl Med, 2015, 7(284): 284ra57 . Doxorubicin (Adriamycin) HCl purchased from Selleck.

    Cell viabilities with increasing concentrations of cisplatin (CP) and doxorubicin (DOXO) under normoxic and hypoxic condition for 48 hours were determined by MTT assay. IC50 values are presented as the means ?SDs (n=4) and * denotes p<0.05.

    Cancer Res 2014 74, 298-308. Doxorubicin (Adriamycin) HCl purchased from Selleck.

  • Cleavage of caspase-3, -9 and the expression of PARP were measured by western blot using b-actin as a loading control.

    Cell Cycle, 2017.. Doxorubicin (Adriamycin) HCl purchased from Selleck.

    (C) & (D) Treatments with doxorubicin, cisplatin or AZD6244 further decreased the cell viability in RH30 cloned cells in which STAT3 or GP130 was knockdown by shRNA. (*P<.05, ** P <.01, *** P <.001).

    Curr Cancer Drug Targets, 2016, 16(7):631-8. Doxorubicin (Adriamycin) HCl purchased from Selleck.

  •  

    Doxorubicin release of P(MAA-g-EG) and P(MAA-g-EG) containing nanoparticles in two-step pH conditions. Doxorubicinloaded P(MAA-g-EG) ,P(MAA-g-EG)-1.0NP ,P(MAA-g-EG)-2.5NP, and P(MAA-g-EG)-5.0NP crushed particles (75–150 um) were released in 1 PBS (pH 2.0) for 90 min. Then the pH was increased to 7.0 by adding 5N NaOH and release continued for 6 h.

    J Biomed Mater Res Part A 2012 10.1002/jbm.a.34064. Doxorubicin (Adriamycin) HCl purchased from Selleck.

    Temporal uptake of free DOX or DOX-PCL63-b-PNVP90 micelles into K-562 (I) and DL (J) cells were studied by incubating in 24-well plates at a concentration of 50,000 cells per well. Representative images shown were obtained using a fluorescence microscope Eclipse 80i (Nikon, Japan) (Plan Fluor, 40X, NA 0.75 objective) equipped with blue and red filters for, Hoechst and Doxorubicin respectively (n = 3).

    PLoS One 2014 9, e94309. Doxorubicin (Adriamycin) HCl purchased from Selleck.

  • PLoS One 2013 8(1), e54595. Doxorubicin (Adriamycin) HCl purchased from Selleck.

    CON+ -positive control (with FBS as chemoattractant); CON- - negative control (without chemoattractant); DOX -doxorubicin; WZ -WZ811; PF- PF-573228; * -significant difference compared to positive control: p<0.05; ** -significant difference compared to positive control: p<0.01; # -significant difference between combination and inhibitor only: p<0.05. Cancer cell invasion was analyzed using Transwell membranes assembled in 24-well plates and covered with a layer of Matrigel. COR-L23 cells were treated for 24 h with 50 nM DOX, 10 uM WZ811, 5 uM PF-573228 and combinations of inhibitors with 50 nM DOX. Similarly, RH460 cells were treated for 24 h with 1 uM DOX, 10 uM WZ811, 2.5 uM PF-573228 and combinations of inhibitors with 1 uM DOX. Applied doses were the ones that exerted the most pronounced synergistic effect on cytotoxicity level. After the incubation period, cells that invaded through matrigel and membranes were fixed in 4% paraformaldehyde-PBS and stained with Hoechst 33342 nuclear stain. Cells were visualized and counted under a fluorescent microscope at 10x magnification.

    2014 Dr Milica Pesic from Institute for Biological Research. Doxorubicin (Adriamycin) HCl purchased from Selleck.

Purity & Quality Control

Choose Selective Topoisomerase Inhibitors

Biological Activity

Description Doxorubicin (Adriamycin) HCl is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage and apoptosis in tumor cells.
Targets
Topo II [1]
(Tumor cells)
In vitro

Doxorubicin, an antibiotic anthracycline, is commonly considered to exert its anti-tumor activity at two fundamental levels, altering DNA and producing free radicals to trigger apoptosis of cancer cells through DNA damage. Doxorubicin can block the synthesis of DNA by intercalating into the DNA strand, and inhibits DNA topoisomerase II (TOP2). Doxorubicin is most effective when cells are rapidly proliferating and expressing high levels of TOP2. Additionally, Doxorubicin can trigger apoptosis by producing ceramide (which prompts apoptosis by activating p53 or other downstream pathways such as JNK), the degradation of Akt by serine threonine proteases, the mitochondrial release of cytochrome c, increased FasL (death receptor Fas/CD95 ligand) mRNA production, and a greater production of free radicals. [2] Pre-treatment with GSNO (nitrosoglutathione) suppresses the resistance in the doxorubicin-resistant breast cancer cell line MCF7/Dx, accompanied by enhanced protein glutathionylation and accumulation of doxorubicin in the nucleus. [3] Doxorubicin induced G2/M checkpoint arrest are attributed to elevated cyclin G2 (CycG2) expression and phospho-modification of proteins in the ataxia telangiectasia mutated (ATM) and ATM and Rad3-related (ATR) signaling pathways. [5] Doxorubicin inhibits AMP-activated protein kinase (AMPK), resulting in SIRT1 dysfunction, p53 accumulation, and increased cell death in mouse embryonic fibroblasts (MEFs) and cardiomyocytes, which can be further sensitized by pre-inhibition of AMPK. [6] Doxorubicin elicits a marked heat shock response, and that either inhibition or silencing of heat shock proteins enhance the Doxorubicin apoptotic effect in neuroblastoma cells. Nanomolar Doxorubicin treatment of neuroblastoma cells causes dose-dependent over-ubiquitination of a specific set of proteins in the absence of measurable inhibition of proteasome, and loss of activity of ubiquitinated enzymes such as lactate dehydrogenase and α-enolase, the protein ubiquination patterns of which is similar to those with proteasome inhibitor Bortezomib, indicating that Doxorubicin may also exert its effect by damaging proteins. [8]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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HOP62 MlyyR5l1d3SxeHnjJGF{e2G7 MmjiO|IhcA>? MVXJR|UxRTBwMEC0JO69VQ>? MkHuPVg4OzZ4MR?=
SF-295 M1:0SWN6fG:2b4jpZ{BCe3OjeR?= M1roWFczKGh? M2TTXGlEPTB;MD6wN|Uh|ryP NWLwXm5WQTh5M{[2NS=>
MDA-MB-361 M1zqd2N6fG:2b4jpZ{BCe3OjeR?= M4LoTFEhcA>? M4PyXmROW09? NYLMRnMyS3m2b4TvfIlkcXS7IHHnZYlve3RiTVTBMW1DNTN4MTDlfJBz\XO|aX7nJGxi[1pid3n0bEBKSzVyIH;mJFAvODF{IN88US=> M4LDdFExOzl3NEmw
MDA-MB-361 MljJR5l1d3SxeHnjJGF{e2G7 M1HBXFEhcA>? NXizNndlTE2VTx?= Mnv0R5l1d3SxeHnjbZR6KGGpYXnud5QhVUSDIF3CJFM3OSCneIDy[ZN{cW6pIHPhdoJwgHmyZYD0bYRie2Vic4TDVGczKHerdHigTWM2OCCxZjCwMlAyQCEQvF2= MVKxNFM6PTR7MB?=
CCRF-SB MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXzlNGJyPCCm NXvRcFlnTE2VTx?= NVLLWYJlUUN3ME2wMlAyKM7:TR?= NX;RW4U4OTB2MUG0O|Y>
CHO-K1 M3PhOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFPhNlQ1KGR? M1faXWROW09? NGXwZYpKSzVyPUCuOEDPxE1? MVmxNFQyOTR5Nh?=
KB M325eWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVrpSpo{PCCm NYjtVIRPTE2VTx?= NWPsZXA5SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBlenWpLYLld4l{fGGwdDDLRldFKHerdHigTWM2OCCxZjCwMlY2KM7:TR?= NH63WGUyODRzMUS3Oi=>
KB NFLsSIZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGnlcZQ1KGR? NF[3PHpFVVOR NH3ZVpFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIRzfWdvcnXzbZN1[W62IFvCR4FueHerdHigTWM2OCCxZjCwMlA3KM7:TR?= NEPVc4EyODRzMUS3Oi=>
KB MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmewOEBl Moe1SG1UVw>? NH\aRopCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIRzfWdvcnXzbZN1[W62IFvCUWRTKHerdHigTWM2OCCxZjCwMlUh|ryP M1TaPFExPDFzNEe2
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MEL-AC MYTDfZRwfG:6aXOgRZN{[Xl? MUOxNFAh|ryP NYHieooyUUN3ME2xMlIh|ryP NGnFTlIyODR5Nki2NS=>
DU145 M3\SfWN6fG:2b4jpZ{BCe3OjeR?= NEXs[Wc4OiCq NUf4c|FvUUN3ME2wMlA2OjhizszN MUmxNFQ4QTJ6Mh?=
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PC14 NWP0Rmt3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn3zTWM2OD1zLkmg{txO MXmxNFY6QDR|Nh?=
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U937 M1rve2Z2dmO2aX;uJGF{e2G7 NGDsdFYzPCCq NEnabWpFVVOR MVjJcohq[mm2czDpcoNwenCxcnH0bY9vKG:oIGuzTH11cHmvaXTpcoUhf2m2aDDJR|UxKG:oIECuNUDPxE1? NVnGbGFWOTB5OEC5NVE>
H460pv8/eto NYXFU|lOS3m2b4TvfIlkKEG|c3H5 MV6xNFAh|ryP NYKx[IZqPDhiaB?= M4\uTHNidGmwZR?= NH:0RpdKSzVyPUCuNFIh|ryP NFLhTpMyODd6MEmxNy=>
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MDA-231 NX7FdJd3S3m2b4TvfIlkKEG|c3H5 NUnTZnZNOTByIN88US=> MWS0PEBp MmOwV4FtcW6n MWjDfZRwfG:6aXPpeJkh[WejaX7zeEBOTEFvMkOxJINmdGxiKFXSMUxGT0[UKzzteZRidnRicEWzLUB4cXSqIFnDOVAhd2ZiMD6wNFYh|ryP NHPTfJYyODd6MEmxNy=>
MDA-468 M{DmOGN6fG:2b4jpZ{BCe3OjeR?= NFLGUVQyODBizszN MXe0PEBp M1ryTHNidGmwZR?= Mni5R5l1d3SxeHnjbZR6KGGpYXnud5QhVUSDLUS2PEBk\WyuIDjFVk0tKGGvcHzp[olm\CCHR1\SMEBufXSjboSgdFU{MSC5aYToJGlEPTBib3[gNE4xODVizszN MWSxNFc5ODlzMx?=
NCI-H226 MluwR5l1d3SxeHnjJGF{e2G7 NV\WcmlvOTByIN88US=> MmnJOFghcA>? MVfTZYxqdmV? NXTTflllS3m2b4TvfIlkcXS7IHHnZYlve3RiTlPJMWgzOjZiY3XscEApdXW2YX70JJA2Oylid3n0bEBKSzVyIH;mJFAvODB6IN88US=> MlLXNVA4QDB7MUO=
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MALME-3M MXjDfZRwfG:6aXOgRZN{[Xl? MljRNVAxKM7:TR?= M2HFXlQ5KGh? MofQS2k2OD1yLkGyJO69VQ>? NXOzdWl{OjN6N{G5NFU>
NCI-H226 M2jufmN6fG:2b4jpZ{BCe3OjeR?= MYCxNFAh|ryP NYjYN4VNPDhiaB?= NXjqRnI3T0l3ME2wMlA2KM7:TR?= NHPleYEzOzh5MUmwOS=>
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AS49-RAW M3PTXWN6fG:2b4jpZ{BCe3OjeR?= M1:1fFExOCEQvF2= NUjwOGtbPDhiaB?= MoXNTWM2OD1|Mj63PEDPxE1? M3y0e|I1ODd5NUK4
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LNcaP Ml7iSpVv[3Srb36gRZN{[Xl? M1rSWlMh|ryP MUKyOEBp NILtXG1KdmO{ZXHz[UBw\iClbHXheoVlKGOjc4Dhd4UuQSCuZY\lcC=> MmHUNlQxQTVyOEm=
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NCI-H1437 NV\HN|RNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXLJR|UxRTZ2Lk[0JI5O MXXTRW5ITVJ?
U251 M1m5ZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2XLZmlEPTB;NkSuPFkhdk1? NFLaR3FUSU6JRWK=
ALL-PO NF\mPFFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUjJR|UxRTZ3LkCzJI5O NIj6THFUSU6JRWK=
NCI-SNU-1 NF75VHpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYXJR|UxRTZ3Lki2JI5O M3\aWXNCVkeHUh?=
RPMI-6666 M{LBOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3vseWlEPTB;NkWuPVkhdk1? MYXTRW5ITVJ?
DBTRG-05MG M2fRbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGPneW9KSzVyPU[3MlEyKG6P NXzxdJZXW0GQR1XS
PF-382 M3SxXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV34[ZRvUUN3ME22O{4yPyCwTR?= MXXTRW5ITVJ?
GT3TKB NGrLOoZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{G0NWlEPTB;NkeuNVkhdk1? MWLTRW5ITVJ?
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CAL-33 M{[3NGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVXJR|UxRTZ5Lki3JI5O MVjTRW5ITVJ?
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CPC-N MmnyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHv4XHdKSzVyPU[4MlQhdk1? MkDLV2FPT0WU
A2780 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml60TWM2OD14OD63PUBvVQ>? M4fWUHNCVkeHUh?=
Daudi M4TpNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWjJR|UxRTZ6Lkm2JI5O MVnTRW5ITVJ?
GI-1 MoTES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUPJR|UxRTZ7Lk[1JI5O MnH4V2FPT0WU
SW962 M3XWdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYLjc4Q3UUN3ME22PU46PSCwTR?= Ml3jV2FPT0WU
MFE-296 NVPJWZlDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{fHSmlEPTB;N{CuOFkhdk1? MYnTRW5ITVJ?
H4 MmjlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NU\mNI5lUUN3ME23NU42PiCwTR?= NGTYR4pUSU6JRWK=
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HCC1599 NIHYRXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXfJR|UxRTd{LkW0JI5O NIfwR4lUSU6JRWK=
HuCCT1 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGT0dnJKSzVyPUeyMlk{KG6P MUDTRW5ITVJ?
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DU-145 MmPXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{fBdmlEPTB;N{OuO|Mhdk1? NVXuT2hKW0GQR1XS
IST-SL2 Mn\2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUXOcFltUUN3ME23N{45OSCwTR?= M3nCbXNCVkeHUh?=
HSC-3 M4Pq[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NES3T3BKSzVyPUe0MlU{KG6P M2PJOHNCVkeHUh?=
HCC1806 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4DDTGlEPTB;N{SuPVYhdk1? Mki0V2FPT0WU
VM-CUB-1 MmfIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnTkTWM2OD15NT6zOkBvVQ>? Ml3HV2FPT0WU
HSC-4 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYjMOJQ4UUN3ME23OU44PiCwTR?= M4\UVHNCVkeHUh?=
NH-12 NX\ycoJ5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnzKTWM2OD15NjDuUS=> MXfTRW5ITVJ?
MDA-MB-468 NIHOeWpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NG\ib5lKSzVyPUe2MlE5KG6P M3TXTnNCVkeHUh?=
TUR NFq2UldIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFTsN3RKSzVyPUe2MlMzKG6P NFTvTGdUSU6JRWK=
TE-1 NELP[5RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4rMU2lEPTB;N{[uN|Uhdk1? MkHyV2FPT0WU
CMK M{PGTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXKyfJdUUUN3ME23O{41OiCwTR?= NVizNFdDW0GQR1XS
LB996-RCC NEH2WINIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1HK[GlEPTB;N{iuOFUhdk1? NEfSTWdUSU6JRWK=
NCI-H1650 M2nX[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmjNTWM2OD15OD62NkBvVQ>? NInSWWhUSU6JRWK=
DMS-273 NHjCZ5VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVrJR|UxRTd6Lkmgcm0> MmH5V2FPT0WU
NOS-1 NVfvSnFZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2DpWmlEPTB;N{muNVQhdk1? NVLuNYx{W0GQR1XS
NB69 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXXJR|UxRTd7LkKgcm0> MXnTRW5ITVJ?
SUP-T1 MkTHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYX6PJo6UUN3ME23PU44OiCwTR?= M4rJXHNCVkeHUh?=
A172 NImzWFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1P2fGlEPTB;OECuOVUhdk1? NVXZSm01W0GQR1XS
TE-12 MnvqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2\NU2lEPTB;OEGuNFYhdk1? M2rE[HNCVkeHUh?=
MKN45 MknPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYHJR|UxRThzLkWxJI5O NUKxUJRLW0GQR1XS
LoVo M1r0WGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn7JTWM2OD16MT64NkBvVQ>? MlzTV2FPT0WU
SW1573 NFvhUmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXHJR|UxRTh{LkixJI5O NUD3UFBJW0GQR1XS
ETK-1 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFfuRpFKSzVyPUizMlAyKG6P NEnhU5VUSU6JRWK=
ABC-1 M17iNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV;JR|UxRTh|LkS1JI5O MYXTRW5ITVJ?
COLO-668 MlrxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUHJR|UxRTh2LkS3JI5O NFHPOYlUSU6JRWK=
UM-UC-3 NULNUllFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4n0VWlEPTB;OE[uNFIhdk1? M2DafXNCVkeHUh?=
BC-1 NUeycIhWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUHs[3dCUUN3ME24Ok44PiCwTR?= MUDTRW5ITVJ?
PANC-10-05 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVHJR|UxRTh6LkS1JI5O M4fT[nNCVkeHUh?=
ES3 M3LKNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{jDW2lEPTB;OEmuOFghdk1? M3HrdnNCVkeHUh?=
TE-441-T MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFrE[GtKSzVyPUi5MlgzKG6P M1XkTXNCVkeHUh?=
KM-H2 NXj4eoNuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIPTTWpKSzVyPUmwMlY5KG6P M4GxXnNCVkeHUh?=
SH-4 NUnISXVLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M331[GlEPTB;OUKuNVEhdk1? NYTGNnFnW0GQR1XS
SF268 NH\wSpdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEL5V3BKSzVyPUmyMlQzKG6P NYHYO5pxW0GQR1XS
VMRC-RCZ M3joSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH7vOHpKSzVyPUmzMlI3KG6P MnLwV2FPT0WU
GI-ME-N NH7IfGRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXTGb3FlUUN3ME25N{4{OyCwTR?= M4ezXXNCVkeHUh?=
JEG-3 NWGzdmd7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEC1NVNKSzVyPUm1MlEzKG6P MlHDV2FPT0WU
J-RT3-T3-5 MoXTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NILpcW5KSzVyPUm2MlMyKG6P MWjTRW5ITVJ?
K5 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXHqeJVFUUN3ME25Ok46KG6P M{fpcHNCVkeHUh?=
EFO-27 NX32[ZMzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlyxTWM2OD17Nz60OEBvVQ>? NF;DTFBUSU6JRWK=
SW1783 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3PNc2lEPTB;OUeuPVkhdk1? NX\vVnJ6W0GQR1XS
CAL-39 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnOyTWM2OD17OD6yOEBvVQ>? M2TLNXNCVkeHUh?=
LS-441N NUO2eHhsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlTVTWM2OD17OT6wOkBvVQ>? NVWwSGlIW0GQR1XS
G-402 M{j5cGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVvIUYU4UUN3ME25PU4xQSCwTR?= NYXV[|lvW0GQR1XS
MES-SA M2q1NWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXq5UJViUUN3ME25PU42OyCwTR?= M3LIdHNCVkeHUh?=
FADU NVjKNnNWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M17zR2lEPTB;MUCyMlE5KG6P NGj5[W5USU6JRWK=
DJM-1 NHrtc|ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXzMS2FvUUN3ME2xNFIvOjVibl2= M3XTZ3NCVkeHUh?=
SU-DHL-1 Ml;CS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX7jVXVUUUN3ME2xNFIvPSCwTR?= M1jEXXNCVkeHUh?=
KYSE-410 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{fqe2lEPTB;MUCzMlQ3KG6P MUPTRW5ITVJ?
NCI-H1618 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUT6Z2M5UUN3ME2xNFQvOzVibl2= M4XZc3NCVkeHUh?=
AU565 MlKxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX7JR|UxRTFyND61NUBvVQ>? NWXQRms3W0GQR1XS
SF126 NHT4WmlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWrtVpdvUUN3ME2xNFQvPThibl2= M4POXHNCVkeHUh?=
MMAC-SF M2jvTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFO5bnpKSzVyPUGwOk41QCCwTR?= NIT2V3pUSU6JRWK=
ChaGo-K-1 M4\SOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWXJR|UxRTFyNz65OUBvVQ>? M3j2[nNCVkeHUh?=
NCI-H596 NEj2WoFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1zCXWlEPTB;MUC4MlAyKG6P MXnTRW5ITVJ?
LXF-289 M1rDVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1LXWGlEPTB;MUC5MlI1KG6P M2nMXnNCVkeHUh?=
HCC1569 NFXO[ohIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYC4WpZMUUN3ME2xNFkvOzRibl2= NG\KO2NUSU6JRWK=
NCI-H2170 NITqN3ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXzLZ29sUUN3ME2xNFkvQTJibl2= NUPafGV2W0GQR1XS
ARH-77 MoPrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUnETpFYUUN3ME2xNFkvQTlibl2= MXLTRW5ITVJ?
BOKU MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3nLWGlEPTB;MUGwMlg4KG6P Mm\BV2FPT0WU
SJSA-1 NUmyeVBjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX3JR|UxRTFzMT6wOUBvVQ>? MlXGV2FPT0WU
SW626 NWrYNGJ4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFXpZYxKSzVyPUGxNU44OSCwTR?= MY\TRW5ITVJ?
EHEB MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mnj0TWM2OD1zMUKuOVkhdk1? Mkf6V2FPT0WU
TE-10 NUS4dHdKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1vBNWlEPTB;MUGyMlY5KG6P MnjOV2FPT0WU
MZ7-mel MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUPtSGk3UUN3ME2xNVIvQDRibl2= M13TZ3NCVkeHUh?=
SAS MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVLJR|UxRTFzND60OUBvVQ>? MUTTRW5ITVJ?
TK10 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NW\QeGw2UUN3ME2xNVQvPiCwTR?= MYnTRW5ITVJ?
SW13 NUfEdoZ6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NETaUZNKSzVyPUGxOU4zPiCwTR?= NEHoRZRUSU6JRWK=
HCC1937 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4rhZWlEPTB;MUG1Mlg1KG6P MUPTRW5ITVJ?
SK-NEP-1 MkXVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHPTRYNKSzVyPUGxOk4zPiCwTR?= MWTTRW5ITVJ?
OVCAR-5 NFjDWI5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{nJd2lEPTB;MUG2MlgyKG6P NFuydWtUSU6JRWK=
SW837 NXHzN5NMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF20emdKSzVyPUGxO{46PSCwTR?= M4LuUXNCVkeHUh?=
BFTC-909 NWHwSI1[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1;CTWlEPTB;MUG4MlQ5KG6P MkG3V2FPT0WU
SW872 NYTmZm1vT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH7tTlJKSzVyPUGyNE45OSCwTR?= NGf6WZNUSU6JRWK=
SF539 M4DxdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3;tcGlEPTB;MUKxMlczKG6P NITOb2hUSU6JRWK=
MLMA NY[0cm9tT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVvJR|UxRTF{Mj6wPEBvVQ>? NXv2eI1{W0GQR1XS
BL-41 MkTSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MY\JR|UxRTF{Mj6yNkBvVQ>? NVz0e2tRW0GQR1XS
LB647-SCLC NWDyNppST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYjJR|UxRTF{Mz6zOUBvVQ>? MXXTRW5ITVJ?
NCI-H1703 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlXZTWM2OD1zMk[uOFIhdk1? MYLTRW5ITVJ?
KURAMOCHI NFPr[|FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlnlTWM2OD1zMk[uO|Yhdk1? NE\zVGtUSU6JRWK=
ATN-1 M1vkXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{TpWmlEPTB;MUK3Mlchdk1? NG\NOXlUSU6JRWK=
SK-CO-1 NIX4TnNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NILXWW9KSzVyPUGyPU4zOSCwTR?= M{O2UnNCVkeHUh?=
RPMI-7951 NEfLeJlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYXaOotpUUN3ME2xN|AvOTRibl2= M1i0OHNCVkeHUh?=
NCI-H1648 MkLBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnfFTWM2OD1zM{GuPVQhdk1? M2PXfXNCVkeHUh?=
NCI-H64 NIf1U2RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M13UNGlEPTB;MUOyMlY2KG6P M2i4bHNCVkeHUh?=
HT-3 NVW4T5N[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFW2WnRKSzVyPUGzN{4xPCCwTR?= MVHTRW5ITVJ?
NCI-H1770 M{HvUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGS1OHdKSzVyPUGzN{4zOSCwTR?= NFfMd4pUSU6JRWK=
NB13 MkDiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4LqZ2lEPTB;MUO0MlU2KG6P M4fvSXNCVkeHUh?=
NCI-H747 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEfjVJpKSzVyPUGzOU4xOiCwTR?= M4DyVnNCVkeHUh?=
ML-2 MljmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1TyWWlEPTB;MUO1MlE3KG6P MWTTRW5ITVJ?
CA46 NHn6PXdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MojjTWM2OD1zM{[uPFghdk1? MlTRV2FPT0WU
ES6 NUK2XG45T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlH2TWM2OD1zM{euNVIhdk1? MnPiV2FPT0WU
JiyoyeP-2003 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4naWGlEPTB;MUO3Mlghdk1? MVrTRW5ITVJ?
NMC-G1 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWXJR|UxRTF|OT6xN{BvVQ>? NX3Gbmx[W0GQR1XS
U-698-M NGWwVnNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4C3TWlEPTB;MUO5MlUhdk1? NXLTOYh5W0GQR1XS
LU-99A MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVTJR|UxRTF2Nj61OUBvVQ>? M{TJU3NCVkeHUh?=
NCI-H748 MmraS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYfJR|UxRTF2Nz6zNUBvVQ>? MXTTRW5ITVJ?
SK-MEL-3 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHHWNpNKSzVyPUG0O{43OSCwTR?= NUjsVIFiW0GQR1XS
SNB75 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmO0TWM2OD1zNEiuOlUhdk1? MWPTRW5ITVJ?
BB65-RCC M{jpdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MorzTWM2OD1zNEmuN|Ehdk1? NIi3VZJUSU6JRWK=
NCI-H1355 NEDlZZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV20[WwyUUN3ME2xOVIvOjRibl2= MUHTRW5ITVJ?
SCC-25 MnLFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUnWRphmUUN3ME2xOVMvPzNibl2= NHfITmVUSU6JRWK=
OAW-42 NIHrXnZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUfJR|UxRTF3Mz64OEBvVQ>? M1LsOHNCVkeHUh?=
MONO-MAC-6 M2i5Nmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF;xfWhKSzVyPUG1OE4{PiCwTR?= NXq4flJmW0GQR1XS
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NCI-H2405 NFzoXG9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHG1UndKSzVyPUKzOE4yKG6P M1;IOHNCVkeHUh?=
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TE-11 NV3rOphUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGDEeZRKSzVyPUK0NE45QCCwTR?= NVflb5JrW0GQR1XS
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HH M2LPTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX;XbY9CUUN3ME2yOFEvPDZibl2= Mk\MV2FPT0WU
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AM-38 NE\SNGpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mnr4TWM2OD1{OEGuOVIhdk1? M3SySnNCVkeHUh?=
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GOTO M{POcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlPWTWM2OD1{OEWuOVYhdk1? M{f3dHNCVkeHUh?=
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LB373-MEL-D NGj1Ro9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1;QXGlEPTB;M{S0MlY1KG6P NFzTVmpUSU6JRWK=
LU-139 NITNWWNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHHwXlhKSzVyPUO0OE46OiCwTR?= MVjTRW5ITVJ?
U-87-MG NH;Cfo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVvsZnVCUUN3ME2zOFYvODRibl2= NXfoPXZmW0GQR1XS
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SNU-475 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1O1O2lEPTB;Mj65NlU4OiEQvF2= MlG5V2FPT0WU
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YAPC M3raTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX3MbJBVUUN3ME2zMlMzQDN{IN88US=> M3TYR3NCVkeHUh?=
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Mewo NWDs[5NIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX21eI9tUUN3ME2zMlQ3ODh4IN88US=> MV7TRW5ITVJ?
COLO-824 M{P0VWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVPJNG9KUUN3ME2zMlU4Ozd6IN88US=> M1nTSnNCVkeHUh?=
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NCI-H345 NX7BfFVjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmDKTWM2OD12LkCxNVc1KM7:TR?= MX;TRW5ITVJ?
COR-L88 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4O5TWlEPTB;ND6zPVU5KM7:TR?= NInqblVUSU6JRWK=
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JVM-3 NVjoN5lwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHOydYNKSzVyPUSuOlA3OThizszN NIjUZWxUSU6JRWK=
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DMS-53 MljxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYT4d|JkUUN3ME22MlE4OTB4IN88US=> MnP3V2FPT0WU
LB771-HNC NHvQeFdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkLnTWM2OD14LkS2PFA3KM7:TR?= NWXDZ5dIW0GQR1XS
BB49-HNC M4OwV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1jWTGlEPTB;Nz60NVc6OSEQvF2= MY\TRW5ITVJ?
MDA-MB-415 NYfVUll1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV\JR|UxRTdwNUezNFMh|ryP NVPtclhMW0GQR1XS
TALL-1 NH:1eYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYnJR|UxRTdwOU[4PEDPxE1? NX7Ie2NtW0GQR1XS
HCC1395 M1rQbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXnJR|UxRThwMUWxNlIh|ryP MUnTRW5ITVJ?
EFE-184 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUDJR|UxRThwM{eyNVYh|ryP MVXTRW5ITVJ?
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A498 M{LaT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MorWTWM2OD1zOD6yPVc3KM7:TR?= NFzRb|RUSU6JRWK=
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SNU-C1 MlrES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWTJR|UxRTN{LkSxO|Uh|ryP M3[5dHNCVkeHUh?=
C32 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1jnd2lEPTB;M{KuPFczKM7:TR?= MUnTRW5ITVJ?
SW684 M{T6eWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoTPTWM2OD1|ND6xOVYyKM7:TR?= M3TuNXNCVkeHUh?=
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... Click to View More Cell Line Experimental Data

In vivo In vivo, Doxorubicin in combination with adenoviral MnSOD (AdMnSOD) plus 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) has the greatest effect in decreasing the volumes of MB231 tumors and prolonging survival of mice. [1] Although its use is limited by the chronic and acute toxic side effects it produces, Doxorubicin is essential in treating breast and oesophageal carcinomas, solid tumours in childhood, osteosarcomas, Kaposi's sarcoma, soft tissue sarcomas, and Hodgkin and non-Hodgkin lymphomas. [2]

Protocol

Animal Research:[1]
+ Expand
  • Animal Models: Female athymic nude mice injected s.c. with MB231 cells
  • Formulation: Dissolved in DMSO, and diluted in saline
  • Dosages: 3 mg/kg/day
  • Administration: Delivered intratumorly
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (172.41 mM)
Water 20 mg/mL (34.48 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 579.98
Formula

C27H29NO11.HCl

CAS No. 25316-40-9
Storage powder
Synonyms NSC 123127

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03036904 Not yet recruiting Diffuse Large B-Cell Lymphoma|High Grade B-Cell Lymphoma Weill Medical College of Cornell University|Genentech, Inc.|Massachusetts General Hospital|M.D. Anderson Cancer Center February 6, 2017 Phase 1
NCT02659930 Recruiting Kaposi Sarcoma National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) January 4, 2016 Phase 1
NCT03042819 Not yet recruiting Soft Tissue Sarcoma University Health Network, Toronto|Karyopharm Therapeutics, Inc March 27, 2017 Phase 1
NCT03033914 Recruiting Hodgkin Lymphoma Memorial Sloan Kettering Cancer Center|Bristol-Myers Squibb|M.D. Anderson Cancer Center|City of Hope Medical Center January 25, 2017 Phase 1
NCT02957968 Recruiting Locally Advanced Breast Cancer|Invasive Adenocarcinoma of the Breast|Breast Cancer Stage II|Breast Cancer Stage III|Breast Cancer Stage IV Virginia Commonwealth University|Merck Sharp & Dohme Corp.|National Cancer Institute (NCI) January 24, 2017 Phase 2
NCT02377752 Active, not recruiting Neoplasm Eli Lilly and Company March 23, 2015 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    Why the IC50 of the compound varied over time in our measurement?

  • Answer:

    Sometimes the IC50 value might vary between different batch of compounds due to the slight differences in purity. If different IC50 was seen in the same batch of compound, the problem may lies in the process of dissolvement. Make sure that you see no particles and the solution is clear with your stock solution.

Topoisomerase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID