Doxorubicin (Adriamycin) HCl

Licensed by Pfizer Catalog No.S1208 Synonyms: NSC 123127

Doxorubicin (Adriamycin) HCl Chemical Structure

Molecular Weight(MW): 579.98

Doxorubicin (Adriamycin) HCl is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage and apoptosis in tumor cells.

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Cited by 31 Publications

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  • Enhancement of apoptotic response by addition of targeted agents lapatinib and sunitinib to doxorubicin in the same device reservoir at 24 hours. P values were determined using Student’s t test for all graphs.

    Sci Transl Med, 2015, 7(284): 284ra57 . Doxorubicin (Adriamycin) HCl purchased from Selleck.

    Cell viabilities with increasing concentrations of cisplatin (CP) and doxorubicin (DOXO) under normoxic and hypoxic condition for 48 hours were determined by MTT assay. IC50 values are presented as the means ?SDs (n=4) and * denotes p<0.05.

    Cancer Res 2014 74, 298-308. Doxorubicin (Adriamycin) HCl purchased from Selleck.

  • Cleavage of caspase-3, -9 and the expression of PARP were measured by western blot using b-actin as a loading control.

    Cell Cycle, 2017.. Doxorubicin (Adriamycin) HCl purchased from Selleck.

    (C) & (D) Treatments with doxorubicin, cisplatin or AZD6244 further decreased the cell viability in RH30 cloned cells in which STAT3 or GP130 was knockdown by shRNA. (*P<.05, ** P <.01, *** P <.001).

    Curr Cancer Drug Targets, 2016, 16(7):631-8. Doxorubicin (Adriamycin) HCl purchased from Selleck.

  •  

    Doxorubicin release of P(MAA-g-EG) and P(MAA-g-EG) containing nanoparticles in two-step pH conditions. Doxorubicinloaded P(MAA-g-EG) ,P(MAA-g-EG)-1.0NP ,P(MAA-g-EG)-2.5NP, and P(MAA-g-EG)-5.0NP crushed particles (75–150 um) were released in 1 PBS (pH 2.0) for 90 min. Then the pH was increased to 7.0 by adding 5N NaOH and release continued for 6 h.

    J Biomed Mater Res Part A 2012 10.1002/jbm.a.34064. Doxorubicin (Adriamycin) HCl purchased from Selleck.

    Temporal uptake of free DOX or DOX-PCL63-b-PNVP90 micelles into K-562 (I) and DL (J) cells were studied by incubating in 24-well plates at a concentration of 50,000 cells per well. Representative images shown were obtained using a fluorescence microscope Eclipse 80i (Nikon, Japan) (Plan Fluor, 40X, NA 0.75 objective) equipped with blue and red filters for, Hoechst and Doxorubicin respectively (n = 3).

    PLoS One 2014 9, e94309. Doxorubicin (Adriamycin) HCl purchased from Selleck.

  • PLoS One 2013 8(1), e54595. Doxorubicin (Adriamycin) HCl purchased from Selleck.

    CON+ -positive control (with FBS as chemoattractant); CON- - negative control (without chemoattractant); DOX -doxorubicin; WZ -WZ811; PF- PF-573228; * -significant difference compared to positive control: p<0.05; ** -significant difference compared to positive control: p<0.01; # -significant difference between combination and inhibitor only: p<0.05. Cancer cell invasion was analyzed using Transwell membranes assembled in 24-well plates and covered with a layer of Matrigel. COR-L23 cells were treated for 24 h with 50 nM DOX, 10 uM WZ811, 5 uM PF-573228 and combinations of inhibitors with 50 nM DOX. Similarly, RH460 cells were treated for 24 h with 1 uM DOX, 10 uM WZ811, 2.5 uM PF-573228 and combinations of inhibitors with 1 uM DOX. Applied doses were the ones that exerted the most pronounced synergistic effect on cytotoxicity level. After the incubation period, cells that invaded through matrigel and membranes were fixed in 4% paraformaldehyde-PBS and stained with Hoechst 33342 nuclear stain. Cells were visualized and counted under a fluorescent microscope at 10x magnification.

    2014 Dr Milica Pesic from Institute for Biological Research. Doxorubicin (Adriamycin) HCl purchased from Selleck.

Purity & Quality Control

Choose Selective Topoisomerase Inhibitors

Biological Activity

Description Doxorubicin (Adriamycin) HCl is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage and apoptosis in tumor cells.
Targets
Topo II [1]
(Tumor cells)
In vitro

Doxorubicin, an antibiotic anthracycline, is commonly considered to exert its anti-tumor activity at two fundamental levels, altering DNA and producing free radicals to trigger apoptosis of cancer cells through DNA damage. Doxorubicin can block the synthesis of DNA by intercalating into the DNA strand, and inhibits DNA topoisomerase II (TOP2). Doxorubicin is most effective when cells are rapidly proliferating and expressing high levels of TOP2. Additionally, Doxorubicin can trigger apoptosis by producing ceramide (which prompts apoptosis by activating p53 or other downstream pathways such as JNK), the degradation of Akt by serine threonine proteases, the mitochondrial release of cytochrome c, increased FasL (death receptor Fas/CD95 ligand) mRNA production, and a greater production of free radicals. [2] Pre-treatment with GSNO (nitrosoglutathione) suppresses the resistance in the doxorubicin-resistant breast cancer cell line MCF7/Dx, accompanied by enhanced protein glutathionylation and accumulation of doxorubicin in the nucleus. [3] Doxorubicin induced G2/M checkpoint arrest are attributed to elevated cyclin G2 (CycG2) expression and phospho-modification of proteins in the ataxia telangiectasia mutated (ATM) and ATM and Rad3-related (ATR) signaling pathways. [5] Doxorubicin inhibits AMP-activated protein kinase (AMPK), resulting in SIRT1 dysfunction, p53 accumulation, and increased cell death in mouse embryonic fibroblasts (MEFs) and cardiomyocytes, which can be further sensitized by pre-inhibition of AMPK. [6] Doxorubicin elicits a marked heat shock response, and that either inhibition or silencing of heat shock proteins enhance the Doxorubicin apoptotic effect in neuroblastoma cells. Nanomolar Doxorubicin treatment of neuroblastoma cells causes dose-dependent over-ubiquitination of a specific set of proteins in the absence of measurable inhibition of proteasome, and loss of activity of ubiquitinated enzymes such as lactate dehydrogenase and α-enolase, the protein ubiquination patterns of which is similar to those with proteasome inhibitor Bortezomib, indicating that Doxorubicin may also exert its effect by damaging proteins. [8]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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Yoshida sarcoma NUfxSW1tTnWwY4Tpc44hSXO|YYm= MUXJcohq[mm2aX;uJI9nKFt|SG3U[HIhcW6lb4Lwc5JifGmxbjD3bZRpKEmFNUCgc4YhOC5yMjFOwG0> MWqxN|IzQTh4
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T-C8166 Mn7rS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MW\JSFUxRTBwMEGg{txO M1fHNFE2PDh4OEG=
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T-MT4 M136OGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVrJSFUxRTBwMEGg{txO NWi3WWlVOTV2OE[4NS=>
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HT-29 M3\OTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkXjNE4xOSEQvHe= NIntXGI1QCCq NV7Z[nFxSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJk> NGTSSoMzPzd6NES5
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C38 NFHvWoNEgXSxdH;4bYMhSXO|YYm= NWLqdY83UUN3ME2wMlAyKM7:TR?= NEfOOXI5ODV5Mkmx
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A2780-DX5 NV7nRolpS3m2b4TvfIlkKEG|c3H5 NVXGWWt5S3m2b4TvfIlkcXS7IHHnZYlve3RiZH;4c5J2[mmlaX6tdoV{cXO2YX70JGEzPzhyLVTYOUB4cXSqIFnDOVAhd2ZiMD6zOVch|ryP MmfYPFg{OTd3NR?=
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MCF MXTGeY5kfGmxbjDBd5NigQ>? MVHS[ZZmenOjbDDv[kBRNWeyLX3l[IlifGWmIH31cJRq\HK3ZzDy[ZNqe3SjbnPlJIF{e2W|c3XkJIF{KGWwaHHuZ4Vu\W62IHnuJIN6fG:2b4jpZ4l1gSCxZjDh[JJq[W27Y3nu Ml7IPFk5PDF3MR?=
C6 MlfYR5l1d3SxeHnjJGF{e2G7 NFj2d2gzNjVibXevcWw> MXu3NkBp NFnuU3pFVVOR MY\DfZRwfG:6aXPpeJkh[WejaX7zeEBz[XRiQ{[gZ4VtdHNiYYSgOVAhfWdxbVygeI8hOi53IH3nM41N NHL3fFY5QTh6NkC0
C6 MWHGeY5kfGmxbjDBd5NigQ>? Moi5Nk42KG2pL33M NYrhd5lQPCCq Mo\LSG1UVw>? MX\Jcohq[mm2aX;uJI9nKHS3YoXsbY4heG:ueX3ldol7[XSrb36= MkfDPFk5QDZyNB?=
C6 M2O3b2N6fG:2b4jpZ{BCe3OjeR?= MWSyMlUhdWdxbVy= NUG5Z|Y3PSCq NGfiN|VFVVOR NEfzd4dEgXSxdH;4bYNqfHliYYPz[ZN{\WRiYYOgZ4VtdCC{ZXzlZZNm M2HlR|g6QDh4MES=
SKVLB MnX4R5l1d3SxeHnjJGF{e2G7 Mo\tO|IhcA>? NH73TXlKSzVyPU[uOVQh|ryP MVS5NFA{PTJy
MCF7/VP16 M1\TVWN6fG:2b4jpZ{BCe3OjeR?= M{\wN|UxKM7:Zz;tUC=> NGPwbog4OiCq MnPETWM2OD1zND6yJO69VQ>? M{GyeFkxOjJ5OUK=
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CHO MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3jYOlExKM7:TR?= NFjsXm43KGR? NEHBfZdKSzVyPUCuNFYh|ryP MlPVPVUzPjV5MB?=
Panc02 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVS1SJpJOTBizszN NWnpOoZUPiCm NWPtXJRlUUN3ME2wMlA3PSEQvF2= NH3KTlE6PTJ4NUew
PC14 NX\yV4N6S3m2b4TvfIlkKEG|c3H5 NWD4ZYIxPCCm NGjYVW9FVVOR MnfLTWM2OD1yLkK5JO69VQ>? M3nafVk5OjJ3NEK=
HOP62 Ml\BR5l1d3SxeHnjJGF{e2G7 MUe3NkBp M{GxXGlEPTB;MD6wNFQh|ryP MWe5PFc{PjZz
SF-295 Ml73R5l1d3SxeHnjJGF{e2G7 Mm\UO|IhcA>? NFj1VXZKSzVyPUCuNFM2KM7:TR?= M{O4c|k5PzN4NkG=
MDA-MB-361 NXzpbGt6S3m2b4TvfIlkKEG|c3H5 NUPwVYRbOSCq NGqyeJlFVVOR MoXpR5l1d3SxeHnjbZR6KGGpYXnud5QhVUSDLV3CMVM3OSCneIDy[ZN{cW6pIFzhZ3ohf2m2aDDJR|UxKG:oIECuNFEzKM7:TR?= NYDzSHFlOTB|OUW0PVA>
MDA-MB-361 M{TVTWN6fG:2b4jpZ{BCe3OjeR?= M{nmOVEhcA>? MnvoSG1UVw>? MofyR5l1d3SxeHnjbZR6KGGpYXnud5QhVUSDIF3CJFM3OSCneIDy[ZN{cW6pIHPhdoJwgHmyZYD0bYRie2Vic4TDVGczKHerdHigTWM2OCCxZjCwMlAyQCEQvF2= M3vjXVExOzl3NEmw
CCRF-SB M{DaSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlWxOEBl MX7EUXNQ Ml7oTWM2OD1yLkCxJO69VQ>? NE\FRoQyODRzMUS3Oi=>
CHO-K1 M2H4dmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIDsTHQ1KGR? MW\EUXNQ NXjwdpd3UUN3ME2wMlQh|ryP NHHZcIQyODRzMUS3Oi=>
KB NYLESnpmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoO1OEBl MnSySG1UVw>? MXfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHTyeYcuemW|aYP0ZY51KEuEN1Sge4l1cCCLQ{WwJI9nKDBwNkWg{txO MmrmNVA1OTF2N{[=
KB M1GzfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2\vSlQh\A>? NEHlPHVFVVOR NUjK[ZhNSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBlenWpLYLld4l{fGGwdDDLRmNidXC5aYToJGlEPTBib3[gNE4xPiEQvF2= M1nyelExPDFzNEe2
KB M{DpcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXe0JIQ> NFvpXoFFVVOR M3zEbmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4Sg[JJ2\y2{ZYPpd5RidnRiS1LNSHIhf2m2aDDJR|UxKG:oIECuOUDPxE1? MnvsNVA1OTF2N{[=
KB Mn;0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWm0JIQ> NUDoPGNxTE2VTx?= NYnxe2xzSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBlenWpLYLld4l{fGGwdDDLRnYzOENid3n0bEBKSzVyIH;mJFAvPCEQvF2= MkTGNVA1OTF2N{[=
HTB-54 NF73RnREgXSxdH;4bYMhSXO|YYm= MXmxNFAh|ryP MU\JR|UxRTFwMTFOwG0> Mkn6NVA1PzZ6NkG=
MEL-AC MUPDfZRwfG:6aXOgRZN{[Xl? M1jjRlExOCEQvF2= MoTwTWM2OD1zLkKg{txO MnftNVA1PzZ6NkG=
DU145 Mk\lR5l1d3SxeHnjJGF{e2G7 MoHlO|IhcA>? NFvzOmJKSzVyPUCuNFUzQCEQvF2= NGn4VmsyODR5OUK4Ni=>
K562R NWjYeFZ4S3m2b4TvfIlkKEG|c3H5 MWq3NkBp NXz4XoFmUUN3ME2wMlAyPTZizszN MV2xNFQ4QTJ6Mh?=
PACA-2 NYDsOIljS3m2b4TvfIlkKEG|c3H5 NFzCUVU3KGR? Mo\hSWQ2OD1yLkCwOlMh|ryP NEnk[oUyODR7OEKxOC=>
3Y1 Ml[xR5l1d3SxeHnjJGF{e2G7 NGjDbVA4OiCq NF;wOlVIUTVyPUCuNFA{QCEQvF2= MV:xNFY6OTdzMB?=
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HMV-1 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIT5N5RKSzVyPUCuNlgh|ryP MVmxNFY6QDR|Nh?=
MKN-1 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYi5dVdkUUN3ME2wMlY1KM7:TR?= M1;adFExPjl6NEO2
PC14 M37pZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGnZS4ZKSzVyPUGuPUDPxE1? MkXzNVA3QTh2M{[=
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U937 MU\GeY5kfGmxbjDBd5NigQ>? M4iycFI1KGh? MnfpSG1UVw>? MUnJcohq[mm2czDpcoNwenCxcnH0bY9vKG:oIGuzTH11cHmvaXTpcoUhf2m2aDDJR|UxKG:oIECuNUDPxE1? MUCxNFc5ODlzMR?=
H460pv8/eto NUjJTmNwS3m2b4TvfIlkKEG|c3H5 MlHjNVAxKM7:TR?= NWDjWWY5PDhiaB?= M3y4cnNidGmwZR?= Ml3NTWM2OD1yLkCyJO69VQ>? MYexNFc5ODlzMx?=
H460pv8 MnThR5l1d3SxeHnjJGF{e2G7 NFHWPWcyODBizszN NE\s[XU1QCCq M2KzTnNidGmwZR?= NXXi[3dnUUN3ME2wMlAxPCEQvF2= M2\FZ|ExPzhyOUGz
MDA-231 M3S4bWN6fG:2b4jpZ{BCe3OjeR?= MYCxNFAh|ryP MofjOFghcA>? NHWwPXZU[WyrbnW= MkXwR5l1d3SxeHnjbZR6KGGpYXnud5QhVUSDLUKzNUBk\WyuIDjFVk0tTUeIUjuscZV1[W62IIC1N{khf2m2aDDJR|UxKG:oIECuNFA3KM7:TR?= NYHZdoZiOTB5OEC5NVM>
MDA-468 MlvNR5l1d3SxeHnjJGF{e2G7 MlvrNVAxKM7:TR?= NHr5c2Y1QCCq NX6wWY9MW2GuaX7l NHfESFJEgXSxdH;4bYNqfHliYXfhbY5{fCCPRFGtOFY5KGOnbHygLGVTNSxiYX3wcIlncWWmIFXHSnItKG23dHHueEBxPTNrIIfpeIghUUN3MDDv[kAxNjByNTFOwG0> M4\2blExPzhyOUGz
NCI-H226 NWXPO3F1S3m2b4TvfIlkKEG|c3H5 NEjNOGcyODBizszN MVi0PEBp NHrqUGlU[WyrbnW= NUDLT|RuS3m2b4TvfIlkcXS7IHHnZYlve3RiTlPJMWgzOjZiY3XscEApdXW2YX70JJA2Oylid3n0bEBKSzVyIH;mJFAvODB6IN88US=> MljMNVA4QDB7MUO=
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BOKU MkPjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml6xTWM2OD1zMUCuPFchdk1? MY\TRW5ITVJ?
SJSA-1 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MomyTWM2OD1zMUGuNFUhdk1? MU\TRW5ITVJ?
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TE-10 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVnHZXlVUUN3ME2xNVIvPjhibl2= MlvtV2FPT0WU
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NCI-H1703 NF3xOZlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{XRbGlEPTB;MUK2MlQzKG6P M3rHNXNCVkeHUh?=
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RPMI-7951 NVXBZllvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFnlfoNKSzVyPUGzNE4yPCCwTR?= M{\qVHNCVkeHUh?=
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NCI-H1770 NHLQc4tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoewTWM2OD1zM{OuNlEhdk1? M4ThdXNCVkeHUh?=
NB13 M{LzVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYPWWG1IUUN3ME2xN|QvPTVibl2= NVLkZY1HW0GQR1XS
NCI-H747 M{fMbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWDJR|UxRTF|NT6wNkBvVQ>? M1LRfHNCVkeHUh?=
ML-2 M2rNemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVrJR|UxRTF|NT6xOkBvVQ>? M1;xOnNCVkeHUh?=
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JiyoyeP-2003 MoP3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2rRS2lEPTB;MUO3Mlghdk1? M4nHWXNCVkeHUh?=
NMC-G1 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn:wTWM2OD1zM{muNVMhdk1? NH\UbnFUSU6JRWK=
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LU-99A MkHpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mkj2TWM2OD1zNE[uOVUhdk1? MnXQV2FPT0WU
NCI-H748 NVLUe4l4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWK1bWZVUUN3ME2xOFcvOzFibl2= M13J[HNCVkeHUh?=
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SNB75 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Moj3TWM2OD1zNEiuOlUhdk1? NVvQeHlsW0GQR1XS
BB65-RCC MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVjxfYVNUUN3ME2xOFkvOzFibl2= NIPwOmpUSU6JRWK=
NCI-H1355 M2fOZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmXtTWM2OD1zNUKuNlQhdk1? NIHCOo5USU6JRWK=
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OAW-42 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkTlTWM2OD1zNUOuPFQhdk1? MVfTRW5ITVJ?
MONO-MAC-6 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmHFTWM2OD1zNUSuN|Yhdk1? MV7TRW5ITVJ?
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NCI-H2347 Mm\DS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmfJTWM2OD1zNUiuN|Qhdk1? MV3TRW5ITVJ?
CAMA-1 NYnnVXF6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXPJR|UxRTF3OT6zN{BvVQ>? MXzTRW5ITVJ?
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NCI-H2052 NUHJ[mt6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYjvVotZUUN3ME2xOlAvPDdibl2= NU\3WG9kW0GQR1XS
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A4-Fuk MoCyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoXPTWM2OD1zNkKuOFghdk1? M4XQVHNCVkeHUh?=
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BC-3 M2fOSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4T0T2lEPTB;MU[1Mlg3KG6P MXHTRW5ITVJ?
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SJRH30 MlfES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWXrWoRqUUN3ME2xO|IvQTRibl2= NGntTopUSU6JRWK=
HEL Mn;JS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGq3R5hKSzVyPUG3OU4yPSCwTR?= M{nrenNCVkeHUh?=
KLE MmPhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWjPNWd1UUN3ME2xO|UvPDdibl2= M4\FVXNCVkeHUh?=
MEG-01 M2XK[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGC1dpBKSzVyPUG3Ok43KG6P NGj2W|BUSU6JRWK=
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COLO-741 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3fremlEPTB;MUm0MlU5KG6P MmLHV2FPT0WU
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L-540 M1XXRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmHjTWM2OD1{MUOuOFkhdk1? M4jpb3NCVkeHUh?=
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HO-1-N-1 M{G4Zmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWrJR|UxRTJ{Mj64N{BvVQ>? M17hdnNCVkeHUh?=
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NY MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M172N2lEPTB;MkK4MlQ6KG6P NEfBUY5USU6JRWK=
NCI-H2196 NI\mbY9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkfuTWM2OD1{MkmuNVUhdk1? NGDhXnZUSU6JRWK=
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Detroit562 NXnCcXFPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlL3TWM2OD1{M{CuO|Ihdk1? M{TDeHNCVkeHUh?=
NCI-H2405 MkH5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXHqVZduUUN3ME2yN|QvOSCwTR?= M{jYb3NCVkeHUh?=
NCI-H2081 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3vXbWlEPTB;MkO0MlI4KG6P MUfTRW5ITVJ?
LB2241-RCC NIDlNoxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYfiRWtIUUN3ME2yN|UvOTFibl2= NI\BbJNUSU6JRWK=
SNU-387 NVfhTXRvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoPZTWM2OD1{M{[uNFghdk1? M1jMU3NCVkeHUh?=
PC-14 MnzaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGLGem5KSzVyPUKzOk4yOiCwTR?= MmfUV2FPT0WU
CAS-1 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHO4TZpKSzVyPUKzPE4{OSCwTR?= Ml;HV2FPT0WU
TE-11 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXvJR|UxRTJ2MD64PEBvVQ>? MmfDV2FPT0WU
LC4-1 NHP3U5lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGiybJBKSzVyPUK0NE45QSCwTR?= NHPEVndUSU6JRWK=
HH M2\kNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX\mZ|EzUUN3ME2yOFEvPDZibl2= Ml\2V2FPT0WU
JVM-2 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHfueGFKSzVyPUK0Nk4yPyCwTR?= M3S3c3NCVkeHUh?=
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T-24 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1y3WmlEPTB;MkS1MlQ5KG6P MoHvV2FPT0WU
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BV-173 MmjoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXvxfXBIUUN3ME2yOVAvPzdibl2= NEXGNYhUSU6JRWK=
EW-18 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX:yd4NzUUN3ME2yOVAvQSCwTR?= NGH3XYJUSU6JRWK=
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Calu-6 MoTxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mmf1TWM2OD1{NUOuOVUhdk1? MYXTRW5ITVJ?
GR-ST NUXvR5p7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGrpfI1KSzVyPUK1OU41OiCwTR?= MlT3V2FPT0WU
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TE-9 MlHrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3jJPWlEPTB;MkW3MlQ4KG6P NF\BOXNUSU6JRWK=
HuH-7 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX\xTIFuUUN3ME2yOVgvPDRibl2= MmPqV2FPT0WU
KYSE-520 NH:0ZlFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoLWTWM2OD1{NkCuO|chdk1? NWqzRpZtW0GQR1XS
NCI-H520 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlztTWM2OD1{Nk[uOlEhdk1? NHjOdWJUSU6JRWK=
HD-MY-Z M3jxRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF72TWhKSzVyPUK3Nk44QSCwTR?= NF;yNGZUSU6JRWK=
SW756 NHy0WYxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3G0R2lEPTB;Mke0Mlc5KG6P M1P5dnNCVkeHUh?=
NB12 Mof3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWjuOVE1UUN3ME2yO|cvOTJibl2= NYrtTY1kW0GQR1XS
EW-3 NXLYRndLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2qzSGlEPTB;Mke5Mlg6KG6P NFu0eINUSU6JRWK=
AM-38 NGPtbXdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{fkU2lEPTB;MkixMlUzKG6P NEOxTpBUSU6JRWK=
NCI-H23 M3jCXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHeyR2RKSzVyPUK4N{46OiCwTR?= NIjYOolUSU6JRWK=
GOTO NGm0N2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVGwclVYUUN3ME2yPFUvPTZibl2= MYTTRW5ITVJ?
NCI-H1693 M2\vbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYrJR|UxRTJ7MD6zNkBvVQ>? NWXP[mhsW0GQR1XS
MOLT-16 NEfjUFdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIH2ZnpKSzVyPUK5NE42KG6P MmfRV2FPT0WU
HOP-92 NF7NPXBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3v3WGlEPTB;MkmwMlchdk1? MkfKV2FPT0WU
MDA-MB-361 NFTWUGVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYjJR|UxRTJ7Mj62OUBvVQ>? NEH0UINUSU6JRWK=
Ramos-2G6-4C10 NGrGR5JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkLMTWM2OD1{OUSuOVUhdk1? MXXTRW5ITVJ?
CAL-54 NG\lXoFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M37IU2lEPTB;M{CwMlc{KG6P M{\RXHNCVkeHUh?=
D-263MG MorHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX;3XYREUUN3ME2zNFUvPTJibl2= Mnm5V2FPT0WU
SKG-IIIa Mn7uS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4rvT2lEPTB;M{GxMlE1KG6P M3e0bHNCVkeHUh?=
HAL-01 M4XjbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFLGd5NKSzVyPUOxN{41PCCwTR?= M3fTWHNCVkeHUh?=
LAN-6 Mnm4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV\1TmNwUUN3ME2zNVUvOTFibl2= M2qx[3NCVkeHUh?=
NCI-H1304 M{C2c2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4X2OGlEPTB;M{KxMlE4KG6P M1j2eHNCVkeHUh?=
no-11 NUWzcphjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{C0WWlEPTB;M{KxMlY1KG6P NGrWbWNUSU6JRWK=
IST-MES1 M1[zNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXTJR|UxRTN{Mz6zOUBvVQ>? M{PlfnNCVkeHUh?=
L-363 Mk\sS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWHJR|UxRTN{ND60N{BvVQ>? NIXweG9USU6JRWK=
EM-2 NXz2[olCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX7Jc4xsUUN3ME2zNlUvPDFibl2= M3foSXNCVkeHUh?=
DV-90 MmXUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2rNbmlEPTB;M{K5MlQ2KG6P M4PlNHNCVkeHUh?=
Saos-2 NEXIRo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXq2TGFrUUN3ME2zNlkvQTRibl2= M2K3U3NCVkeHUh?=
UMC-11 NV7kelJOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mk\STWM2OD1|M{GuNlghdk1? NIfFc3hUSU6JRWK=
Raji MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUjJR|UxRTN|ND62OEBvVQ>? M{PlbHNCVkeHUh?=
RXF393 NI\mdnBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYnJR|UxRTN|NT60N{BvVQ>? M{e0PXNCVkeHUh?=
EW-11 Ml:1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MULJR|UxRTN2MT61OkBvVQ>? MlT3V2FPT0WU
BL-70 M2f2W2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYTGNnNoUUN3ME2zOFIvODFibl2= NEHWTW1USU6JRWK=
LB373-MEL-D MnrnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3jtWmlEPTB;M{S0MlY1KG6P NHmxdotUSU6JRWK=
LU-139 M1HCbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlzxTWM2OD1|NESuPVIhdk1? NGexXFRUSU6JRWK=
U-87-MG NVm0NplqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1LXcWlEPTB;M{S2MlA1KG6P Mnj5V2FPT0WU
LU-65 NXXM[5VWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHHJdYJKSzVyPUO0Ok41KG6P MoPWV2FPT0WU
U031 M2r0W2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NELOd3RKSzVyPUO1N{45PyCwTR?= NWruZoQ5W0GQR1XS
HN NXXweXlLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYjpR5IyUUN3ME2zOVQvOjdibl2= NUDsOmtmW0GQR1XS
MHH-CAL-2 M3X2O2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX;JR|UxRTN3OT6yNUBvVQ>? MVTTRW5ITVJ?
NCI-H510A MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXvaNpA6UUN3ME2zOVkvPTlibl2= NIXxRotUSU6JRWK=
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COLO-792 M3X1Tmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVLJR|UxRTNwMEK4O{DPxE1? NH\0UXpUSU6JRWK=
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COR-L88 NF3yXGVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGflXnZKSzVyPUSuN|k2QCEQvF2= M{n0fnNCVkeHUh?=
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NCI-H2227 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXn0NJN[UUN3ME21MlM3OjNzIN88US=> M3[zZXNCVkeHUh?=
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LB771-HNC MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2XMVmlEPTB;Nj60OlgxPiEQvF2= NV7FPXZwW0GQR1XS
BB49-HNC NFPnR49Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGfKSIFKSzVyPUeuOFE4QTFizszN NHj2fohUSU6JRWK=
MDA-MB-415 MkP6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{TJOGlEPTB;Nz61O|MxOyEQvF2= M3vaVnNCVkeHUh?=
TALL-1 NW\udW0{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NILiUZNKSzVyPUeuPVY5QCEQvF2= M{jxNnNCVkeHUh?=
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SiHa NH;UeHRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV6yRlJkUUN3ME2yN{4{OzdzIN88US=> NVSxbFd4W0GQR1XS
RCC10RGB MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnrOTWM2OD1|MD6wOlEyKM7:TR?= MlPTV2FPT0WU
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P31-FUJ M2HnNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlLITWM2OD12ND6zNlg1KM7:TR?= NGHTOI1USU6JRWK=
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... Click to View More Cell Line Experimental Data

In vivo In vivo, Doxorubicin in combination with adenoviral MnSOD (AdMnSOD) plus 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) has the greatest effect in decreasing the volumes of MB231 tumors and prolonging survival of mice. [1] Although its use is limited by the chronic and acute toxic side effects it produces, Doxorubicin is essential in treating breast and oesophageal carcinomas, solid tumours in childhood, osteosarcomas, Kaposi's sarcoma, soft tissue sarcomas, and Hodgkin and non-Hodgkin lymphomas. [2]

Protocol

Animal Research:[1]
+ Expand
  • Animal Models: Female athymic nude mice injected s.c. with MB231 cells
  • Formulation: Dissolved in DMSO, and diluted in saline
  • Dosages: 3 mg/kg/day
  • Administration: Delivered intratumorly
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (172.41 mM)
Water 20 mg/mL (34.48 mM)
Ethanol slightly soluble or insoluble
In vivo Add solvents individually and in order:

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 579.98
Formula

C27H29NO11.HCl

CAS No. 25316-40-9
Storage powder
in solvent
Synonyms NSC 123127

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03036904 Not yet recruiting Diffuse Large B-Cell Lymphoma|High Grade B-Cell Lymphoma Weill Medical College of Cornell University|Genentech, Inc.|Massachusetts General Hospital|M.D. Anderson Cancer Center February 6, 2017 Phase 1
NCT02659930 Recruiting Kaposi Sarcoma National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) January 4, 2016 Phase 1
NCT03042819 Not yet recruiting Soft Tissue Sarcoma University Health Network, Toronto|Karyopharm Therapeutics, Inc March 27, 2017 Phase 1
NCT03033914 Recruiting Hodgkin Lymphoma Memorial Sloan Kettering Cancer Center|Bristol-Myers Squibb|M.D. Anderson Cancer Center|City of Hope Medical Center January 25, 2017 Phase 1
NCT02957968 Recruiting Locally Advanced Breast Cancer|Invasive Adenocarcinoma of the Breast|Breast Cancer Stage II|Breast Cancer Stage III|Breast Cancer Stage IV Virginia Commonwealth University|Merck Sharp & Dohme Corp.|National Cancer Institute (NCI) January 24, 2017 Phase 2
NCT02377752 Active, not recruiting Neoplasm Eli Lilly and Company March 23, 2015 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Topoisomerase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID