Geneticin (G418 Sulfate)

Catalog No.S3028

Geneticin (G418 Sulfate) Chemical Structure

Molecular Weight(MW): 692.71

Geneticin (G418 Sulfate), an aminoglycoside antibiotic, is an elongation inhibitor of 80 S ribosomes that blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells.

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Biological Activity

Description Geneticin (G418 Sulfate), an aminoglycoside antibiotic, is an elongation inhibitor of 80 S ribosomes that blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells.
In vitro

G418 is an inhibitor of many pro- and eukaryotes at concentrations from 1-300 microgram/ml. Resistance to G418 conferring by the neo gene from Tn5 encoding an aminoglycoside 3'-phosphotransferase, APT 3' II is commonly used in laboratory research to select genetically engineered cells. [1] In general for bacteria and algae concentrations of 5 mg/L or less are used, for mammalian cells concentrations of approximately 400 mg/L are used for selection and 200 mg/L for maintenance. Resistant clones selection could require from 1 to up to 3 weeks. [2]

In vivo Doses of G418 of between 40 and 80 mg/kg for three consecutive days are sufficient to eliminate all nontransfected T. brucei brucei parasites from infected mice. [3]

Protocol

Animal Research:

[3]

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  • Animal Models: Sublethally irradiated mice with bloodstream forms of T. bruceibrucei GUTat 3.1 and T. brucei brucei GUTat 3.1/BBR3.
  • Formulation: In sterile water
  • Dosages: 10, 20, 30, 40, 50, or 80 mg/kg
  • Administration: I.P.
    (Only for Reference)

Solubility (25°C)

In vitro Water 100 mg/mL warmed (144.36 mM)
DMSO <1 mg/mL
Ethanol <1 mg/mL
In vivo Saline 30 mg/mL

* 1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 692.71
Formula

C20H40N4O10.2H2O4S

CAS No. 108321-42-2
Storage powder
in solvent
Synonyms N/A

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID