Geneticin (G418 Sulfate)

Geneticin (G418 Sulfate), an aminoglycoside antibiotic, is an elongation inhibitor of 80 S ribosomes that blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells.It is commonly used as a screening reagent for eukaryotic cells to screen transfected or transformed cells.

Geneticin (G418 Sulfate) Chemical Structure

Geneticin (G418 Sulfate) Chemical Structure

CAS: 108321-42-2

Selleck's Geneticin (G418 Sulfate) has been cited by 21 publications

Purity & Quality Control

Batch: Purity: >97%
97

Geneticin (G418 Sulfate) Related Products

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Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
GES-1 cells Function assay be used to select and expand the G418-resistant colonies 29963172
A549 cells Cell viability assay 100-1200 μg/ml IC50=494.1 μg/ml 30594785
Sf21 cells Function assay 60 mins Inhibition of ribosomal activity in Spodoptera frugiperda Sf21 cells assessed as inhibition of translation after 60 mins by fluorescence assay, IC50 = 2 μM. 20409719
Click to View More Cell Line Experimental Data

Biological Activity

Description Geneticin (G418 Sulfate), an aminoglycoside antibiotic, is an elongation inhibitor of 80 S ribosomes that blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells.It is commonly used as a screening reagent for eukaryotic cells to screen transfected or transformed cells.
In vitro
In vitro

G418 is an inhibitor of many pro- and eukaryotes at concentrations from 1-300 microgram/ml. Resistance to G418 conferring by the neo gene from Tn5 encoding an aminoglycoside 3'-phosphotransferase, APT 3' II is commonly used in laboratory research to select genetically engineered cells. [1]

In general for bacteria and algae concentrations of 5 mg/L or less are used, for mammalian cells concentrations of approximately 400 mg/L are used for selection and 200 mg/L for maintenance. Resistant clones selection could require from 1 to up to 3 weeks. [2]

In Vivo
In vivo

Doses of G418 of between 40 and 80 mg/kg for three consecutive days are sufficient to eliminate all nontransfected T. brucei brucei parasites from infected mice. [3]

Animal Research Animal Models Sublethally irradiated mice with bloodstream forms of T. bruceibrucei GUTat 3.1 and T. brucei brucei GUTat 3.1/BBR3.
Dosages 10, 20, 30, 40, 50, or 80 mg/kg
Administration I.P.

Chemical Information & Solubility

Molecular Weight 692.71 Formula

C20H40N4O10.2H2SO4

CAS No. 108321-42-2 SDF Download Geneticin (G418 Sulfate) SDF
Smiles CC(C1C(C(C(C(O1)OC2C(CC(C(C2O)OC3C(C(C(CO3)(C)O)NC)O)N)N)N)O)O)O.OS(=O)(=O)O.OS(=O)(=O)O
Storage (From the date of receipt)

In vitro
Batch:

Water : 100 mg/mL

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : Insoluble


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In vivo
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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