Embelin

Catalog No.S7025 Synonyms: Embelic Acid, NSC 91874

Embelin Chemical Structure

Molecular Weight(MW): 294.39

Embelin, a quinone isolated from the Japanese Ardisia herb, is an inhibitor of X-linked inhibitor of apoptosis (XIAP) with IC50 of 4.1 μM in a cell-free assay.

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3 Customer Reviews

  • (d) Representative plots of apoptosis in Jurkat cells treated with vehicle only, BIO, CN585, Embelin (Emb), BIO plus CN585 or BIO plus CN585 plus Emb.

    Leukemia, 2016, 812-822.. Embelin purchased from Selleck.

    Histological evaluation of distal ileum, lung, and liver. CLP led to organ injuries with increased neutrophil infiltration. Embelin treatment resulted in improved morphology in ileum, lung, and liver with reduced neutrophil infiltration (H&E stain, ×200).

    Inflammation, 2015, 38(4): 1556-1562.. Embelin purchased from Selleck.

  • Inhibition of XIAP by its inhibitor Embelin leads to sensitizing MDA-MB-231 cells to cisplatin.

    EBioMedicine, 2017, 15:48-61. Embelin purchased from Selleck.

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Biological Activity

Description Embelin, a quinone isolated from the Japanese Ardisia herb, is an inhibitor of X-linked inhibitor of apoptosis (XIAP) with IC50 of 4.1 μM in a cell-free assay.
Targets
5-LO [2]
(Cell-free assay)
mPGES-1 [2]
(Cell-free assay)
XIAP [1]
(Cell-free assay)
0.06 μM 0.2 μM 4.1 μM
In vitro

Embelin from the Japanese Ardisia herb is a small-molecular inhibitor that binds to the XIAP BIR3 domain with which Smac and caspsase-9 bind. Embelin inhibits cell growth of both PC-3 and LNCap cells in a dose-dependent manner, with IC50 values of 3.7 and 5.7 μM. While, the toxicity of Embelin in normal PrEC and in WI-38 cells is much lower with IC50 values of 20.1 μM and 19.3 μM. Treatment of PC-3 cells with 25 and 50 μM of embelin for 48 h, 30% and 75% of cells undergo apoptosis, representing approximately 3-and 9-fold increase as compared to untreated cells. [1] Embelin also potently suppresses the biosynthesis of eicosanoids by selective inhibition of 5-lipoxygenase (5-LO) and Microsomal prostaglandin E2 synthase-1 (mPGES-1) with IC50 = 0.06 and 0.2 mM, respectively. [2]

Protocol

Kinase Assay:[1]
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Fluorescence Polarization Competitive Binding Assay:

Fluorescence polarization experiments are performed in Dynex 96-well, black, round-bottom plates. A 5 μL sample of Embelin dilutions in DMSO, and preincubated XIAP BIR3 protein (0.06 μM) and the N terminus of a Smac peptide (SM7F) (0.01 μM) in the assay buffer are added to 96-well plates to produce a final volume of 125 μL. For each assay, the bound peptide control containing XIAP BIR3 protein and SM7F (equivalent to 0% inhibition) and free peptide control containing only free SM7F (equivalent to 100% inhibition) were included. The plates were mixed and incubated at room temperature for 3 h to reach equilibrium.
Cell Research:[1]
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  • Cell lines: Human prostate cancer cell lines (PC-3, LNCap), normal human fibroblast cell line WI-38, and humanprostate epithelial cells (PrEC)
  • Concentrations: 1-64 μM
  • Incubation Time: 4-5 days
  • Method: Cell growth is determined by the MTT-based assay using Cell Proliferation Reagent WST-1 according to the manufacturer’s instruction. Cells (5000 cells/well) are grown in medium with 10% FBS, and various concentrations of Embelin are added to the cells. Four to five days later, WST-1 is added to each well and incubated for 1-3 h at 37 °C. Absorbance is measured with a plate reader at 450 nm with correction at 650 nm.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 59 mg/mL (200.41 mM)
Ethanol 12 mg/mL (40.76 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 294.39
Formula

C17H26O4

CAS No. 550-24-3
Storage powder
Synonyms Embelic Acid, NSC 91874

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Related Antibodies

IAP Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID