Geneticin (G418 Sulfate)

Catalog No.S3028 Batch:S302815

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Technical Data

Formula

C20H40N4O10.2H2SO4

Molecular Weight 692.71 CAS No. 108321-42-2
Solubility (25°C)* In vitro Water 100 mg/mL (144.36 mM)
DMSO Insoluble
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
Saline
30.0mg/ml Taking the 1 mL working solution as an example, add 30 mg of this product to 1 ml of physiological saline (0.9% NaCL solution), mix evenly to make it clear, The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Geneticin (G418 Sulfate), an aminoglycoside antibiotic, is an elongation inhibitor of 80 S ribosomes that blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells.It is commonly used as a screening reagent for eukaryotic cells to screen transfected or transformed cells.
In vitro

G418 is an inhibitor of many pro- and eukaryotes at concentrations from 1-300 microgram/ml. Resistance to G418 conferring by the neo gene from Tn5 encoding an aminoglycoside 3'-phosphotransferase, APT 3' II is commonly used in laboratory research to select genetically engineered cells. [1]

In general for bacteria and algae concentrations of 5 mg/L or less are used, for mammalian cells concentrations of approximately 400 mg/L are used for selection and 200 mg/L for maintenance. Resistant clones selection could require from 1 to up to 3 weeks. [2]

In vivo

Doses of G418 of between 40 and 80 mg/kg for three consecutive days are sufficient to eliminate all nontransfected T. brucei brucei parasites from infected mice. [3]

Protocol (from reference)

Animal Study:

[3]

  • Animal Models

    Sublethally irradiated mice with bloodstream forms of T. bruceibrucei GUTat 3.1 and T. brucei brucei GUTat 3.1/BBR3.

  • Dosages

    10, 20, 30, 40, 50, or 80 mg/kg

  • Administration

    I.P.

Selleck's Geneticin (G418 Sulfate) has been cited by 24 publications

USP36 stabilizes nucleolar Snail1 to promote ribosome biogenesis and cancer cell survival upon ribotoxic stress [ Nat Commun, 2023, 14(1):6473] PubMed: 37833415
USP36 stabilizes nucleolar Snail1 to promote ribosome biogenesis and cancer cell survival upon ribotoxic stress [ Nat Commun, 2023, 14(1):6473] PubMed: 37833415
TSPAN18 facilitates bone metastasis of prostate cancer by protecting STIM1 from TRIM32-mediated ubiquitination [ J Exp Clin Cancer Res, 2023, 42(1):195] PubMed: 37542345
LMO2 promotes the development of AML through interaction with transcription co-regulator LDB1 [ Cell Death Dis, 2023, 14(8):518] PubMed: 37573405
LMO2 promotes the development of AML through interaction with transcription co-regulator LDB1 [ Cell Death Dis, 2023, 14(8):518] PubMed: 37573405
HIF2α-induced upregulation of RNASET2 promotes triglyceride synthesis and enhances cell migration in clear cell renal cell carcinoma [ FEBS Open Bio, 2023, 13(4):638-654] PubMed: 36728187
HIF2α-induced upregulation of RNASET2 promotes triglyceride synthesis and enhances cell migration in clear cell renal cell carcinoma [ FEBS Open Bio, 2023, none] PubMed: 36728187
A multimodal iPSC platform for cystic fibrosis drug testing [ Nat Commun, 2022, 13(1):4270] PubMed: 35906215
Targeting glutamine metabolism in hepatic stellate cells alleviates liver fibrosis [ Cell Death Dis, 2022, 13(11):955] PubMed: 36376267
Synthetic lethality of cyclin-dependent kinase inhibitor Dinaciclib with VHL-deficiency allows for selective targeting of clear cell renal cell carcinoma [ Cell Cycle, 2022, 1-17] PubMed: 35240916

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.