ZM 447439

Catalog No.S1103

ZM 447439 Chemical Structure

Molecular Weight(MW): 513.59

ZM 447439 is a selective and ATP-competitive inhibitor for Aurora A and Aurora B with IC50 of 110 nM and 130 nM, respectively. It is more than 8-fold selective for Aurora A/B than MEK1, Src, Lck and has little effect against CDK1/2/4, Plk1, Chk1, etc.

Size Price Stock Quantity  
In DMSO USD 98 In stock
USD 70 In stock
USD 110 In stock
USD 270 In stock
USD 470 In stock
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3 Customer Reviews

  • p31comet depletion delays MCC disassembly even with the proteasome inhibited. (A) FACS analysis of HeLa Tet-on cells transfected with control or p31comet siRNA and then treated with Taxol followed by Aurora B inhibition with ZM447439. The percentage of mitotic cells (cells that have 4N DNA content and are MPM2 positive) is shown for each sample.

    Mol Biol Cell 2011 22, 4227-35. ZM 447439 purchased from Selleck.

    HeLa cells were treated with Nocodazole (100ng/ml) (Lanes 2-4) or Taxol (1µM) (Lanes 6-8) or DMSO (lanes 1, 5) for 16h. The indicated inhibitors were added for 2h (1 µM) before harvesting the cells. The p-Aurora-A (T288), B (T232), C (T198) antibody was from Cell Signaling (#2914). MK5108 is an Aurora-A inhibitor. VX680 inhibits all three Aurora kinases. ZM447439 inhibits both Aurora-B and -C kinases, but not Aurora-A kinase.

    Dr. Yuanhong Chen of University of Nebraska. ZM 447439 purchased from Selleck.

  • Western blot analysis of Histone and Aurora kinase. 0-10μM ZM447439 was added.

    Dr. Zhang of Tianjin Medical University. ZM 447439 purchased from Selleck.

Purity & Quality Control

Choose Selective Aurora Kinase Inhibitors

Biological Activity

Description ZM 447439 is a selective and ATP-competitive inhibitor for Aurora A and Aurora B with IC50 of 110 nM and 130 nM, respectively. It is more than 8-fold selective for Aurora A/B than MEK1, Src, Lck and has little effect against CDK1/2/4, Plk1, Chk1, etc.
Features An Aurora selective ATP-competitive inhibitor.
Targets
Aurora A [1]
(Cell-free assay)
Aurora B [1]
(Cell-free assay)
LCK [1]
(Cell-free assay)
Src [1]
(Cell-free assay)
MEK1 [1]
(Cell-free assay)
110 nM 130 nM 880 nM 1.03 μM 1.79 μM
In vitro

In vitro, ZM-447439 selectively inhibits recombinant human Aurora A and B with IC50 values of 110 and 130 nM, respectively, while other protein kinases of diverse structural types including the mitotic kinases CDK1 and PLK1 are inhibited with IC50 values >10 μM. [1] Aurora kinase inhibitor, ZM-447439 time- and dose-dependently inhibits the growth of all three cell lines with IC50 values of 3 μM (BON), 0.9 μM (QGP-1) and 3 μM (MIP-101) after 72 hours of continuous exposure. In addition, ZM-447439 potently induces cell apoptosis by promoting DNA fragmentation and caspase 3 and 7 activation, and arrests GEP-NET cells in the G0 /G1and G2/M phase of the cell cycle. [2] In mouse embryo, inhibition of Aurora kinase activity by ZM-447439 results in abnormalities during mitosis by regulating the phosphorylation of histone H3 serine 10 (H3S10Ph) from G2 to metaphase with different perturbations in each embryonic cycle. [3] A recent study shows that ZM-447439 exhibits growth inhibitory and proapoptotic effect on cervical cancer SiHa cells, and enhances the chemosensitivity to cisplatin. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human EoL-1-cell M4T1Smdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIfiPGNKdmirYnn0bY9vKG:oIHj1cYFvKEWxTD2xMYNmdGxiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkG4Olc5KM7:TR?= MofOV2FPT0WU
MCF7 cell MWLQdo9tcW[ncnH0bY9vKGG|c3H5 MUHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IF3DSlch[2WubDDsbY5mNCCLQ{WwQVAvOTl6IN88US=> MnTGNVY{OzdzMkK=
human P12-ICHIKAWA cell NVKzU4k2T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NIHyZVdKdmirYnn0bY9vKG:oIHj1cYFvKFBzMj3JR2hKU0GZQTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNlI1PDFizszN NHHJcZBUSU6JRWK=
human KARPAS-45 cell M4KyZWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWj1NXl3UW6qaXLpeIlwdiCxZjDoeY1idiCNQWLQRXMuPDViY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkO4NVI5KM7:TR?= MYTTRW5ITVJ?
human ES3 cell MXrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVn3ToJSUW6qaXLpeIlwdiCxZjDoeY1idiCHU{OgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlQ4OzJizszN MnLQV2FPT0WU
human ES8 cell MoLLS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHfCU|NKdmirYnn0bY9vKG:oIHj1cYFvKEWVODDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuOFk5ODZizszN MmrDV2FPT0WU
human TE-11 cell MoTHS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NETEWmhKdmirYnn0bY9vKG:oIHj1cYFvKFSHLUGxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE42OzdyMzFOwG0> NFO2cI5USU6JRWK=
human RS4-11 cell NIPtV4dIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFrvelBKdmirYnn0bY9vKG:oIHj1cYFvKFKVND2xNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvPTV2NDFOwG0> MVPTRW5ITVJ?
human MOLT-16 cell NGrKeXlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4\qc2lvcGmkaYTpc44hd2ZiaIXtZY4hVU:OVD2xOkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvPjB7NkGg{txO NVzVdWhrW0GQR1XS
human RKO cell NEi5fGJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NIXZe5lKdmirYnn0bY9vKG:oIHj1cYFvKFKNTzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuO|A3PTZizszN NIjrOZJUSU6JRWK=
human MV-4-11 cell NVnCWpNGT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NGG2ZY5KdmirYnn0bY9vKG:oIHj1cYFvKE2YLUStNVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjd7NkOg{txO M{HXRXNCVkeHUh?=
human SW954 cell MV\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3PUc2lvcGmkaYTpc44hd2ZiaIXtZY4hW1d7NUSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlg{PjN3IN88US=> MmK2V2FPT0WU
human BE-13 cell MorZS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWrxPIZtUW6qaXLpeIlwdiCxZjDoeY1idiCERT2xN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvQDR2MUig{txO MnPwV2FPT0WU
human MOLT-4 cell MXTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NFL2e4pKdmirYnn0bY9vKG:oIHj1cYFvKE2RTGStOEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvQDl7N{ig{txO NEPpVYRUSU6JRWK=
human NBsusSR cell M{\Gfmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1rjOmlvcGmkaYTpc44hd2ZiaIXtZY4hVkK|dYPTVkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvQTF|OEeg{txO NH33cWNUSU6JRWK=
human H9 cell NF7T[|JIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2rZe2lvcGmkaYTpc44hd2ZiaIXtZY4hUDliY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkmyPVc6KM7:TR?= M2DEWXNCVkeHUh?=
human A172 cell NHPZbGFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M{nnfWlvcGmkaYTpc44hd2ZiaIXtZY4hSTF5MjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuPVg1OTFizszN MYPTRW5ITVJ?
human ES5 cell Mk\XS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NETVWFFKdmirYnn0bY9vKG:oIHj1cYFvKEWVNTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuNFAzPDhizszN MV3TRW5ITVJ?
human SBC-1 cell M3HTO2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MmTHTY5pcWKrdHnvckBw\iCqdX3hckBUSkNvMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuNFM6OjhizszN MlLRV2FPT0WU
human NCI-H209 cell MXXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4CzbmlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyNFkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjF4NkCyJO69VQ>? M4rpZXNCVkeHUh?=
human NKM-1 cell M1THWWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFPKbVRKdmirYnn0bY9vKG:oIHj1cYFvKE6NTT2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4yPjd7ODFOwG0> NHnzUGVUSU6JRWK=
human NCI-H720 cell MWLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M17ofGlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVi3NlAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjJyNkK3JO69VQ>? MlXLV2FPT0WU
human KE-37 cell NUHKU5JwT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYHDeplyUW6qaXLpeIlwdiCxZjDoeY1idiCNRT2zO{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvOjF|OEig{txO NH;FOW1USU6JRWK=
human SW48 cell MlHzS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MnvKTY5pcWKrdHnvckBw\iCqdX3hckBUXzR6IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT6yN|E2PSEQvF2= NXnBe3U6W0GQR1XS
human IST-SL1 cell NGO1OpZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NEfyV4xKdmirYnn0bY9vKG:oIHj1cYFvKEmVVD3TUFEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjNzN{K3JO69VQ>? M1mzXHNCVkeHUh?=
human SK-NEP-1 cell NILJZoNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2jIfGlvcGmkaYTpc44hd2ZiaIXtZY4hW0tvTlXQMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjN4NEm4JO69VQ>? NGTa[GNUSU6JRWK=
human NOMO-1 cell NYnnVGVyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NU\neJJ6UW6qaXLpeIlwdiCxZjDoeY1idiCQT13PMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjN4N{K1JO69VQ>? NWDkcYN4W0GQR1XS
human DOHH-2 cell NXHpTYxKT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MV;Jcohq[mm2aX;uJI9nKGi3bXHuJGRQUEhvMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuOFAzPzZizszN NWnvW5l7W0GQR1XS
human ABC-1 cell NVvQXpFGT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NITrcHZKdmirYnn0bY9vKG:oIHj1cYFvKEGEQz2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU41ODN3MjFOwG0> MmHaV2FPT0WU
human Ramos-2G6-4C10 cell NWjLcHhvT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWnGT2JVUW6qaXLpeIlwdiCxZjDoeY1idiCUYX3vd{0zTzZvNFOxNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPDB4MEWg{txO NV7aW2FtW0GQR1XS
human EM-2 cell MX7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MmDOTY5pcWKrdHnvckBw\iCqdX3hckBGVS1{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT60NVczOSEQvF2= NVXsdlFNW0GQR1XS
human NB14 cell Mnm1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVrJcohq[mm2aX;uJI9nKGi3bXHuJG5DOTRiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLkW1OlIyKM7:TR?= MUfTRW5ITVJ?
human MOLT-13 cell M3XDUWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4exdGlvcGmkaYTpc44hd2ZiaIXtZY4hVU:OVD2xN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPTZ5ME[g{txO MVXTRW5ITVJ?
human ECC10 cell MWTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MonSTY5pcWKrdHnvckBw\iCqdX3hckBGS0NzMDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuOlM{PTNizszN MWLTRW5ITVJ?
human LK-2 cell M{\Gemdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIqzdo1KdmirYnn0bY9vKG:oIHj1cYFvKEyNLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlY1PTl2IN88US=> NVjSNWViW0GQR1XS
human CTB-1 cell NYDocnNnT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVfwbm92UW6qaXLpeIlwdiCxZjDoeY1idiCFVFKtNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPjdyOEKg{txO M1PmRXNCVkeHUh?=
human NCI-H1581 cell MUnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXHJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMUW4NUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPjd3NTFOwG0> NIKx[YJUSU6JRWK=
human COLO-800 cell MWLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NEDucIRKdmirYnn0bY9vKG:oIHj1cYFvKEORTF:tPFAxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS55MEO4NkDPxE1? M4LtbXNCVkeHUh?=
human NB7 cell NWL3OIhtT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYjJcohq[mm2aX;uJI9nKGi3bXHuJG5DPyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFwN{WyPVch|ryP MUXTRW5ITVJ?
human LAMA-84 cell M1zJU2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{XmR2lvcGmkaYTpc44hd2ZiaIXtZY4hVEGPQT24OEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPzV3MjFOwG0> NWHXeZp4W0GQR1XS
human HCT-116 cells M3\5O2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NILy[4JKdmirYnn0bY9vKG:oIHj1cYFvKEiFVD2xNVYh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjhyOUC4JO69VQ>? M3ficHNCVkeHUh?=
SK-UT-1 cell MmfLS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mk\ZTY5pcWKrdHnvckBw\iCqdX3hckBUUy2XVD2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU45OTV|IN88US=> MnK2V2FPT0WU
human H4 cell MV3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2\ZWWlvcGmkaYTpc44hd2ZiaIXtZY4hUDRiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLkixOVc5KM7:TR?= M3LO[XNCVkeHUh?=
human CAL-51 cell NVrJfGs{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mn3TTY5pcWKrdHnvckBw\iCqdX3hckBESUxvNUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlg{QDR3IN88US=> NWrLTlNzW0GQR1XS
human LoVo cells NHHidXJRem:uaX\ldoF1cW:wIHHzd4F6 M4rJZ|czKGh? MlnGRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCOb2\vJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGStZoF{\WRiV2PUPEBz\WGpZX70JIF{e2G7LDDJR|UxRTFwOTFOwG0> NVjnNmlOOjV{N{C0NFM>
human HN cell MYrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2DJNGlvcGmkaYTpc44hd2ZiaIXtZY4hUE5iY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLkmyOVEh|ryP NY\DTXFvW0GQR1XS
human L-363 cell M1f2Z2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWnJcohq[mm2aX;uJI9nKGi3bXHuJGwuOzZ|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT65OVEzKM7:TR?= MmrGV2FPT0WU
human NCI-H747 cell NXLrO|RjT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlriTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEe0O{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvODN|NUOg{txO M1L4WnNCVkeHUh?=
human A498 cell NF25OoFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWLxVI1uUW6qaXLpeIlwdiCxZjDoeY1idiCDNEm4JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk4{PjZ7IN88US=> NETYWpZUSU6JRWK=

... Click to View More Cell Line Experimental Data

Protocol

Kinase Assay:[1]
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In vitro kinase assays :

Recombinant Aurora A and B are expressed as NH2-terminal His6-tagged fusion proteins using a baculovirus expression system. Aurora A is purified by affinity chromatography using Ni-NTA agarose, and Aurora B is purified by ion exchange chromatography using CM Sepharose Fast Flow. 1 ng purified recombinant enzyme is added to a reaction cocktail containing 25 mM Tris-HCl, pH 7.5, 12.5 mM KCl, 2.5 mM NaF, 0.6 mM DTT, 6.25 mM MnCl2, 10 μM peptide substrate, 10 μM for Aurora A or 5 μM ATP for Aurora B, and 0.2 μCi γ-[33P]ATP (specific activity ≥2,500 Ci/mmol), and is then incubated at RT for 60 minutes. Reactions are stopped by addition of 20% phosphoric acid, and the products are captured on P30 nitrocellulose filters and assayed for incorporation of 33P with a BetaplateTM counter. No enzyme and no compound control values are used to determine the concentration of ZM447439, which gave 50% inhibition of enzyme activity. Further details are available on request from Nicholas Keen.
Cell Research:[2]
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  • Cell lines: BON, QGP-1 and MIP-101 cells
  • Concentrations: 0-5 μM
  • Incubation Time: 72 hours
  • Method: Cell number is evaluated by crystal violet staining. In brief, cells in 96-well plates are fixed with 1% glutaraldehyde. Then cells are stained with 0.1% crystal violet. The unbound dye is removed by washing with water. Bound crystal violet is solubilized with 0.2% Triton X-100. Light extinction which increases linearly with the cell number is analyzed at 570 nm using an ELISA reader.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 103 mg/mL (200.54 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 513.59
Formula

C29H31N5O4

CAS No. 331771-20-1
Storage powder
in solvent
Synonyms N/A

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Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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