ZM 447439

Catalog No.S1103

ZM 447439 Chemical Structure

Molecular Weight(MW): 513.59

ZM 447439 is a selective and ATP-competitive inhibitor for Aurora A and Aurora B with IC50 of 110 nM and 130 nM, respectively. It is more than 8-fold selective for Aurora A/B than MEK1, Src, Lck and has little effect against CDK1/2/4, Plk1, Chk1, etc.

Size Price Stock Quantity  
In DMSO USD 98 In stock
USD 70 In stock
USD 110 In stock
USD 270 In stock
USD 470 In stock
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3 Customer Reviews

  • p31comet depletion delays MCC disassembly even with the proteasome inhibited. (A) FACS analysis of HeLa Tet-on cells transfected with control or p31comet siRNA and then treated with Taxol followed by Aurora B inhibition with ZM447439. The percentage of mitotic cells (cells that have 4N DNA content and are MPM2 positive) is shown for each sample.

    Mol Biol Cell 2011 22, 4227-35. ZM 447439 purchased from Selleck.

    HeLa cells were treated with Nocodazole (100ng/ml) (Lanes 2-4) or Taxol (1µM) (Lanes 6-8) or DMSO (lanes 1, 5) for 16h. The indicated inhibitors were added for 2h (1 µM) before harvesting the cells. The p-Aurora-A (T288), B (T232), C (T198) antibody was from Cell Signaling (#2914). MK5108 is an Aurora-A inhibitor. VX680 inhibits all three Aurora kinases. ZM447439 inhibits both Aurora-B and -C kinases, but not Aurora-A kinase.

    Dr. Yuanhong Chen of University of Nebraska. ZM 447439 purchased from Selleck.

  • Western blot analysis of Histone and Aurora kinase. 0-10μM ZM447439 was added.

    Dr. Zhang of Tianjin Medical University. ZM 447439 purchased from Selleck.

Purity & Quality Control

Choose Selective Aurora Kinase Inhibitors

Biological Activity

Description ZM 447439 is a selective and ATP-competitive inhibitor for Aurora A and Aurora B with IC50 of 110 nM and 130 nM, respectively. It is more than 8-fold selective for Aurora A/B than MEK1, Src, Lck and has little effect against CDK1/2/4, Plk1, Chk1, etc.
Features An Aurora selective ATP-competitive inhibitor.
Targets
Aurora A [1]
(Cell-free assay)
Aurora B [1]
(Cell-free assay)
LCK [1]
(Cell-free assay)
Src [1]
(Cell-free assay)
MEK1 [1]
(Cell-free assay)
110 nM 130 nM 880 nM 1.03 μM 1.79 μM
In vitro

In vitro, ZM-447439 selectively inhibits recombinant human Aurora A and B with IC50 values of 110 and 130 nM, respectively, while other protein kinases of diverse structural types including the mitotic kinases CDK1 and PLK1 are inhibited with IC50 values >10 μM. [1] Aurora kinase inhibitor, ZM-447439 time- and dose-dependently inhibits the growth of all three cell lines with IC50 values of 3 μM (BON), 0.9 μM (QGP-1) and 3 μM (MIP-101) after 72 hours of continuous exposure. In addition, ZM-447439 potently induces cell apoptosis by promoting DNA fragmentation and caspase 3 and 7 activation, and arrests GEP-NET cells in the G0 /G1and G2/M phase of the cell cycle. [2] In mouse embryo, inhibition of Aurora kinase activity by ZM-447439 results in abnormalities during mitosis by regulating the phosphorylation of histone H3 serine 10 (H3S10Ph) from G2 to metaphase with different perturbations in each embryonic cycle. [3] A recent study shows that ZM-447439 exhibits growth inhibitory and proapoptotic effect on cervical cancer SiHa cells, and enhances the chemosensitivity to cisplatin. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human EoL-1-cell M1;oOGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MmrETY5pcWKrdHnvckBw\iCqdX3hckBGd0xvMT3j[YxtKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5zOE[3PEDPxE1? MVrTRW5ITVJ?
MCF7 cell MV;Qdo9tcW[ncnH0bY9vKGG|c3H5 NYLWTHQ6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBOS0Z5IHPlcIwhdGmwZTygTWM2OD1yLkG5PEDPxE1? NYPm[HdxOTZ|M{exNlI>
human P12-ICHIKAWA cell M4K1TWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NF\vV2NKdmirYnn0bY9vKG:oIHj1cYFvKFBzMj3JR2hKU0GZQTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNlI1PDFizszN MVPTRW5ITVJ?
human KARPAS-45 cell NGHsR5pIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MoW2TY5pcWKrdHnvckBw\iCqdX3hckBMSVKSQWOtOFUh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjN6MUK4JO69VQ>? NVTIXJhsW0GQR1XS
human ES3 cell NGXNOJBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkC0TY5pcWKrdHnvckBw\iCqdX3hckBGWzNiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkS3N|Ih|ryP M4\RWHNCVkeHUh?=
human ES8 cell NUHCdZJxT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVvveYZrUW6qaXLpeIlwdiCxZjDoeY1idiCHU{igZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlQ6QDB4IN88US=> MXHTRW5ITVJ?
human TE-11 cell NFjUd3NIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1z4V2lvcGmkaYTpc44hd2ZiaIXtZY4hXEVvMUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlU{PzB|IN88US=> MkfxV2FPT0WU
human RS4-11 cell MmjyS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlOwTY5pcWKrdHnvckBw\iCqdX3hckBTWzRvMUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlU2PDRizszN MmTyV2FPT0WU
human MOLT-16 cell NGDNcHhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHTBRVFKdmirYnn0bY9vKG:oIHj1cYFvKE2RTGStNVYh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjZyOU[xJO69VQ>? NUK5WHRRW0GQR1XS
human RKO cell M1ziUmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NF;OdnpKdmirYnn0bY9vKG:oIHj1cYFvKFKNTzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuO|A3PTZizszN NULL[JUzW0GQR1XS
human MV-4-11 cell MUfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NIP4R5NKdmirYnn0bY9vKG:oIHj1cYFvKE2YLUStNVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjd7NkOg{txO MXnTRW5ITVJ?
human SW954 cell M2T5fWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYPxOIlHUW6qaXLpeIlwdiCxZjDoeY1idiCVV{m1OEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvQDN4M{Wg{txO MlXDV2FPT0WU
human BE-13 cell MVXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYj1NmpWUW6qaXLpeIlwdiCxZjDoeY1idiCERT2xN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvQDR2MUig{txO MmDKV2FPT0WU
human MOLT-4 cell MXrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NH\odVRKdmirYnn0bY9vKG:oIHj1cYFvKE2RTGStOEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvQDl7N{ig{txO NGfrO|hUSU6JRWK=
human NBsusSR cell M4S1Z2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MoXqTY5pcWKrdHnvckBw\iCqdX3hckBPSnO3c2PSJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE46OTN6NzFOwG0> MlK0V2FPT0WU
human H9 cell NWLaR2xQT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHS4[GZKdmirYnn0bY9vKG:oIHj1cYFvKEh7IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD65Nlk4QSEQvF2= MVfTRW5ITVJ?
human A172 cell MX3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUfJcohq[mm2aX;uJI9nKGi3bXHuJGEyPzJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkm4OFEyKM7:TR?= NGXj[5dUSU6JRWK=
human ES5 cell NXHnSItVT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3PqbGlvcGmkaYTpc44hd2ZiaIXtZY4hTVN3IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT6wNFI1QCEQvF2= M3fqTHNCVkeHUh?=
human SBC-1 cell NV\uTnlYT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVnBdm1ZUW6qaXLpeIlwdiCxZjDoeY1idiCVQlOtNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvODN7Mkig{txO MnHoV2FPT0WU
human NCI-H209 cell M{PyT2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXrJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMkC5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4yPjZyMjFOwG0> NUfvNmN1W0GQR1XS
human NKM-1 cell M4fhemdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVXpT5hEUW6qaXLpeIlwdiCxZjDoeY1idiCQS12tNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvOTZ5OUig{txO NVO1XJBnW0GQR1XS
human NCI-H720 cell NHPBXHFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYW3cXNpUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFczOCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFwMkC2Nlch|ryP NHvyUJlUSU6JRWK=
human KE-37 cell Mn;GS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MX\Jcohq[mm2aX;uJI9nKGi3bXHuJGtGNTN5IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT6yNVM5QCEQvF2= NYLkeWRVW0GQR1XS
human SW48 cell NVHhXFFWT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{PsUWlvcGmkaYTpc44hd2ZiaIXtZY4hW1d2ODDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuNlMyPTVizszN Mn;rV2FPT0WU
human IST-SL1 cell MnfiS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M{fRN2lvcGmkaYTpc44hd2ZiaIXtZY4hUVOWLWPMNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvOzF5Mkeg{txO NULuUG1IW0GQR1XS
human SK-NEP-1 cell MXLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MofJTY5pcWKrdHnvckBw\iCqdX3hckBUUy2QRWCtNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvOzZ2OUig{txO MlHNV2FPT0WU
human NOMO-1 cell NID0TGdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWLYPXBFUW6qaXLpeIlwdiCxZjDoeY1idiCQT13PMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjN4N{K1JO69VQ>? NXLBfYVWW0GQR1XS
human DOHH-2 cell MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MY\Jcohq[mm2aX;uJI9nKGi3bXHuJGRQUEhvMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuOFAzPzZizszN MWTTRW5ITVJ?
human ABC-1 cell NYjvfHltT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MV;Jcohq[mm2aX;uJI9nKGi3bXHuJGFDSy1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT60NFM2OiEQvF2= M1TYXXNCVkeHUh?=
human Ramos-2G6-4C10 cell M{LXNGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NUTrbmUyUW6qaXLpeIlwdiCxZjDoeY1idiCUYX3vd{0zTzZvNFOxNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPDB4MEWg{txO NWr2cpQ2W0GQR1XS
human EM-2 cell NH74bGhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlfjTY5pcWKrdHnvckBw\iCqdX3hckBGVS1{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT60NVczOSEQvF2= MmTJV2FPT0WU
human NB14 cell NX7IcZdNT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3juXWlvcGmkaYTpc44hd2ZiaIXtZY4hVkJzNDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuOVU3OjFizszN MYjTRW5ITVJ?
human MOLT-13 cell NIew[mxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MWfJcohq[mm2aX;uJI9nKGi3bXHuJG1QVFRvMUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlU3PzB4IN88US=> NIPPcnVUSU6JRWK=
human ECC10 cell NFHyRYpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWP5R4xGUW6qaXLpeIlwdiCxZjDoeY1idiCHQ1OxNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPjN|NUOg{txO MVTTRW5ITVJ?
human LK-2 cell MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MV\Jcohq[mm2aX;uJI9nKGi3bXHuJGxMNTJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLk[0OVk1KM7:TR?= M3rqbXNCVkeHUh?=
human CTB-1 cell NGL0TIlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFf4RZhKdmirYnn0bY9vKG:oIHj1cYFvKEOWQj2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU43PzB6MjFOwG0> Mmf2V2FPT0WU
human NCI-H1581 cell NGfoTGRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXPJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMUW4NUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPjd3NTFOwG0> M{LHVXNCVkeHUh?=
human COLO-800 cell NI\YNoFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mn7sTY5pcWKrdHnvckBw\iCqdX3hckBEV0yRLUiwNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPzB|OEKg{txO NUnGSXhjW0GQR1XS
human NB7 cell M4nDRWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MnTjTY5pcWKrdHnvckBw\iCqdX3hckBPSjdiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLke1Nlk4KM7:TR?= NHj3T3pUSU6JRWK=
human LAMA-84 cell Moe3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NH;leZpKdmirYnn0bY9vKG:oIHj1cYFvKEyDTVGtPFQh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjd3NUKg{txO Ml:1V2FPT0WU
human HCT-116 cells MoqwS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MoLoTY5pcWKrdHnvckBw\iCqdX3hckBJS1RvMUG2JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU45ODlyODFOwG0> NYqw[pJbW0GQR1XS
SK-UT-1 cell MY\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MknGTY5pcWKrdHnvckBw\iCqdX3hckBUUy2XVD2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU45OTV|IN88US=> MUfTRW5ITVJ?
human H4 cell NUjLeI9vT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWjJcohq[mm2aX;uJI9nKGi3bXHuJGg1KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS56MUW3PEDPxE1? NWjxeG1EW0GQR1XS
human CAL-51 cell M1rUUGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFzK[o5KdmirYnn0bY9vKG:oIHj1cYFvKEODTD21NUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvQDN6NEWg{txO NGjnTmlUSU6JRWK=
human LoVo cells NW\IcJgxWHKxbHnm[ZJifGmxbjDhd5NigQ>? M37IW|czKGh? NFzu[WRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFzvWo8h[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWE1j[XOnZDDXV3Q5KHKnYXflcpQh[XO|YYmsJGlEPTB;MT65JO69VQ>? NIPLRlczPTJ5MESwNy=>
human HN cell MUTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NIG5cVRKdmirYnn0bY9vKG:oIHj1cYFvKEiQIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT65NlUyKM7:TR?= MXrTRW5ITVJ?
human L-363 cell MnrzS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmG5TY5pcWKrdHnvckBw\iCqdX3hckBNNTN4MzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuPVUyOiEQvF2= MVvTRW5ITVJ?
human NCI-H747 cell NYTBV3g4T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2HZWWlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVi3OFch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjB|M{WzJO69VQ>? MX;TRW5ITVJ?
human A498 cell NFPRUHdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MX7Jcohq[mm2aX;uJI9nKGi3bXHuJGE1QThiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1{LkO2Olkh|ryP M13HbnNCVkeHUh?=

... Click to View More Cell Line Experimental Data

Protocol

Kinase Assay:[1]
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In vitro kinase assays :

Recombinant Aurora A and B are expressed as NH2-terminal His6-tagged fusion proteins using a baculovirus expression system. Aurora A is purified by affinity chromatography using Ni-NTA agarose, and Aurora B is purified by ion exchange chromatography using CM Sepharose Fast Flow. 1 ng purified recombinant enzyme is added to a reaction cocktail containing 25 mM Tris-HCl, pH 7.5, 12.5 mM KCl, 2.5 mM NaF, 0.6 mM DTT, 6.25 mM MnCl2, 10 μM peptide substrate, 10 μM for Aurora A or 5 μM ATP for Aurora B, and 0.2 μCi γ-[33P]ATP (specific activity ≥2,500 Ci/mmol), and is then incubated at RT for 60 minutes. Reactions are stopped by addition of 20% phosphoric acid, and the products are captured on P30 nitrocellulose filters and assayed for incorporation of 33P with a BetaplateTM counter. No enzyme and no compound control values are used to determine the concentration of ZM447439, which gave 50% inhibition of enzyme activity. Further details are available on request from Nicholas Keen.
Cell Research:[2]
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  • Cell lines: BON, QGP-1 and MIP-101 cells
  • Concentrations: 0-5 μM
  • Incubation Time: 72 hours
  • Method: Cell number is evaluated by crystal violet staining. In brief, cells in 96-well plates are fixed with 1% glutaraldehyde. Then cells are stained with 0.1% crystal violet. The unbound dye is removed by washing with water. Bound crystal violet is solubilized with 0.2% Triton X-100. Light extinction which increases linearly with the cell number is analyzed at 570 nm using an ELISA reader.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 103 mg/mL (200.54 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order:
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 513.59
Formula

C29H31N5O4

CAS No. 331771-20-1
Storage powder
Synonyms N/A

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    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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