ZM 447439

Catalog No.S1103

ZM 447439 Chemical Structure

Molecular Weight(MW): 513.59

ZM 447439 is a selective and ATP-competitive inhibitor for Aurora A and Aurora B with IC50 of 110 nM and 130 nM, respectively. It is more than 8-fold selective for Aurora A/B than MEK1, Src, Lck and has little effect against CDK1/2/4, Plk1, Chk1, etc.

Size Price Stock Quantity  
In DMSO USD 98 In stock
USD 70 In stock
USD 110 In stock
USD 270 In stock
USD 470 In stock
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3 Customer Reviews

  • p31comet depletion delays MCC disassembly even with the proteasome inhibited. (A) FACS analysis of HeLa Tet-on cells transfected with control or p31comet siRNA and then treated with Taxol followed by Aurora B inhibition with ZM447439. The percentage of mitotic cells (cells that have 4N DNA content and are MPM2 positive) is shown for each sample.

    Mol Biol Cell 2011 22, 4227-35. ZM 447439 purchased from Selleck.

    HeLa cells were treated with Nocodazole (100ng/ml) (Lanes 2-4) or Taxol (1µM) (Lanes 6-8) or DMSO (lanes 1, 5) for 16h. The indicated inhibitors were added for 2h (1 µM) before harvesting the cells. The p-Aurora-A (T288), B (T232), C (T198) antibody was from Cell Signaling (#2914). MK5108 is an Aurora-A inhibitor. VX680 inhibits all three Aurora kinases. ZM447439 inhibits both Aurora-B and -C kinases, but not Aurora-A kinase.

    Dr. Yuanhong Chen of University of Nebraska. ZM 447439 purchased from Selleck.

  • Western blot analysis of Histone and Aurora kinase. 0-10μM ZM447439 was added.

    Dr. Zhang of Tianjin Medical University. ZM 447439 purchased from Selleck.

Purity & Quality Control

Choose Selective Aurora Kinase Inhibitors

Biological Activity

Description ZM 447439 is a selective and ATP-competitive inhibitor for Aurora A and Aurora B with IC50 of 110 nM and 130 nM, respectively. It is more than 8-fold selective for Aurora A/B than MEK1, Src, Lck and has little effect against CDK1/2/4, Plk1, Chk1, etc.
Features An Aurora selective ATP-competitive inhibitor.
Targets
Aurora A [1]
(Cell-free assay)
Aurora B [1]
(Cell-free assay)
LCK [1]
(Cell-free assay)
Src [1]
(Cell-free assay)
MEK1 [1]
(Cell-free assay)
110 nM 130 nM 880 nM 1.03 μM 1.79 μM
In vitro

In vitro, ZM-447439 selectively inhibits recombinant human Aurora A and B with IC50 values of 110 and 130 nM, respectively, while other protein kinases of diverse structural types including the mitotic kinases CDK1 and PLK1 are inhibited with IC50 values >10 μM. [1] Aurora kinase inhibitor, ZM-447439 time- and dose-dependently inhibits the growth of all three cell lines with IC50 values of 3 μM (BON), 0.9 μM (QGP-1) and 3 μM (MIP-101) after 72 hours of continuous exposure. In addition, ZM-447439 potently induces cell apoptosis by promoting DNA fragmentation and caspase 3 and 7 activation, and arrests GEP-NET cells in the G0 /G1and G2/M phase of the cell cycle. [2] In mouse embryo, inhibition of Aurora kinase activity by ZM-447439 results in abnormalities during mitosis by regulating the phosphorylation of histone H3 serine 10 (H3S10Ph) from G2 to metaphase with different perturbations in each embryonic cycle. [3] A recent study shows that ZM-447439 exhibits growth inhibitory and proapoptotic effect on cervical cancer SiHa cells, and enhances the chemosensitivity to cisplatin. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human EoL-1-cell M1rIemdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MnTWTY5pcWKrdHnvckBw\iCqdX3hckBGd0xvMT3j[YxtKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5zOE[3PEDPxE1? MlLLV2FPT0WU
MCF7 cell M4\DOnBzd2yrZnXyZZRqd25iYYPzZZk> MVXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IF3DSlch[2WubDDsbY5mNCCLQ{WwQVAvOTl6IN88US=> NULYeVZwOTZ|M{exNlI>
human P12-ICHIKAWA cell NUToRZhJT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1P5dGlvcGmkaYTpc44hd2ZiaIXtZY4hWDF{LVnDTGlMSVeDIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6yNlQ1OSEQvF2= Mn76V2FPT0WU
human KARPAS-45 cell M4fY[2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWnJcohq[mm2aX;uJI9nKGi3bXHuJGtCWlCDUz20OUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOzhzMkig{txO MVTTRW5ITVJ?
human ES3 cell MXXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYDJcohq[mm2aX;uJI9nKGi3bXHuJGVUOyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwNEezNkDPxE1? NFTV[o1USU6JRWK=
human ES8 cell MoLUS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlTXTY5pcWKrdHnvckBw\iCqdX3hckBGWzhiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkS5PFA3KM7:TR?= MX7TRW5ITVJ?
human TE-11 cell NUjveHVvT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3LCbmlvcGmkaYTpc44hd2ZiaIXtZY4hXEVvMUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlU{PzB|IN88US=> NXT2[3VZW0GQR1XS
human RS4-11 cell NFHBcmNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MmC2TY5pcWKrdHnvckBw\iCqdX3hckBTWzRvMUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlU2PDRizszN MlrQV2FPT0WU
human MOLT-16 cell M4nUT2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFLDS3RKdmirYnn0bY9vKG:oIHj1cYFvKE2RTGStNVYh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjZyOU[xJO69VQ>? Ml65V2FPT0WU
human RKO cell MX;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MkfWTY5pcWKrdHnvckBw\iCqdX3hckBTU09iY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkewOlU3KM7:TR?= NIDUOIJUSU6JRWK=
human MV-4-11 cell NU\yWIRrT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NH61[HRKdmirYnn0bY9vKG:oIHj1cYFvKE2YLUStNVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjd7NkOg{txO M2HCO3NCVkeHUh?=
human SW954 cell NEG3SVVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWj2VHVDUW6qaXLpeIlwdiCxZjDoeY1idiCVV{m1OEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvQDN4M{Wg{txO NV64e5ZYW0GQR1XS
human BE-13 cell Mnz4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHu1emdKdmirYnn0bY9vKG:oIHj1cYFvKEKHLUGzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE45PDRzODFOwG0> Mn;6V2FPT0WU
human MOLT-4 cell NUXDZXI4T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1n6cWlvcGmkaYTpc44hd2ZiaIXtZY4hVU:OVD20JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE45QTl5ODFOwG0> NHPscllUSU6JRWK=
human NBsusSR cell NYPrSI93T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlnFTY5pcWKrdHnvckBw\iCqdX3hckBPSnO3c2PSJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE46OTN6NzFOwG0> NV\HSW5XW0GQR1XS
human H9 cell M3;TdGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{jubWlvcGmkaYTpc44hd2ZiaIXtZY4hUDliY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkmyPVc6KM7:TR?= M{XKSXNCVkeHUh?=
human A172 cell NFLYOXpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnXyTY5pcWKrdHnvckBw\iCqdX3hckBCOTd{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD65PFQyOSEQvF2= MoKzV2FPT0WU
human ES5 cell M3PCVGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHrQUmRKdmirYnn0bY9vKG:oIHj1cYFvKEWVNTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuNFAzPDhizszN NGLKc2NUSU6JRWK=
human SBC-1 cell M1PjT2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVr2OGloUW6qaXLpeIlwdiCxZjDoeY1idiCVQlOtNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvODN7Mkig{txO MoX2V2FPT0WU
human NCI-H209 cell NUXM[XlpT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{DiVWlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyNFkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjF4NkCyJO69VQ>? NXLsXm9SW0GQR1XS
human NKM-1 cell MlLFS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmnYTY5pcWKrdHnvckBw\iCqdX3hckBPU01vMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuNVY4QThizszN MlHIV2FPT0WU
human NCI-H720 cell Mn7uS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1TnZWlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVi3NlAh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjJyNkK3JO69VQ>? NIf4NW1USU6JRWK=
human KE-37 cell NILRR25Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2PCS2lvcGmkaYTpc44hd2ZiaIXtZY4hU0VvM{egZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlIyOzh6IN88US=> M37GO3NCVkeHUh?=
human SW48 cell M32xSmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVPJcohq[mm2aX;uJI9nKGi3bXHuJHNYPDhiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLkKzNVU2KM7:TR?= MWXTRW5ITVJ?
human IST-SL1 cell NEHIcndIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MUTJcohq[mm2aX;uJI9nKGi3bXHuJGlUXC2VTEGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlMyPzJ5IN88US=> Ml7wV2FPT0WU
human SK-NEP-1 cell M3;VUGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MnfHTY5pcWKrdHnvckBw\iCqdX3hckBUUy2QRWCtNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvOzZ2OUig{txO M2nu[3NCVkeHUh?=
human NOMO-1 cell MnXvS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHHMe3lKdmirYnn0bY9vKG:oIHj1cYFvKE6RTV:tNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvOzZ5MkWg{txO M2C5NHNCVkeHUh?=
human DOHH-2 cell MV7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3jnZmlvcGmkaYTpc44hd2ZiaIXtZY4hTE:KSD2yJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU41ODJ5NjFOwG0> MnSyV2FPT0WU
human ABC-1 cell NHHncW1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NV;VN5dIUW6qaXLpeIlwdiCxZjDoeY1idiCDQlOtNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPDB|NUKg{txO MmHvV2FPT0WU
human Ramos-2G6-4C10 cell Mn7LS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGHab|FKdmirYnn0bY9vKG:oIHj1cYFvKFKjbX;zMVJIPi12Q{GwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU41ODZyNTFOwG0> NFrUSIRUSU6JRWK=
human EM-2 cell MXTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGXhTIxKdmirYnn0bY9vKG:oIHj1cYFvKEWPLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlQyPzJzIN88US=> M37R[3NCVkeHUh?=
human NB14 cell NWX1SFdXT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mn7uTY5pcWKrdHnvckBw\iCqdX3hckBPSjF2IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT61OVYzOSEQvF2= NXLtWZdtW0GQR1XS
human MOLT-13 cell M2L3XGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NX\le|Y4UW6qaXLpeIlwdiCxZjDoeY1idiCPT1zUMVE{KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS53NkewOkDPxE1? NHPRO2xUSU6JRWK=
human ECC10 cell MVfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2PkXWlvcGmkaYTpc44hd2ZiaIXtZY4hTUOFMUCgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlY{OzV|IN88US=> MlrFV2FPT0WU
human LK-2 cell Mnf3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MnfhTY5pcWKrdHnvckBw\iCqdX3hckBNUy1{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT62OFU6PCEQvF2= MV\TRW5ITVJ?
human CTB-1 cell MnTjS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NEPjVmJKdmirYnn0bY9vKG:oIHj1cYFvKEOWQj2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU43PzB6MjFOwG0> MkTEV2FPT0WU
human NCI-H1581 cell MUXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXfJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMUW4NUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvPjd3NTFOwG0> MkjLV2FPT0WU
human COLO-800 cell MWLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVq3PJp7UW6qaXLpeIlwdiCxZjDoeY1idiCFT1zPMVgxOCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFwN{CzPFIh|ryP NWrnRotSW0GQR1XS
human NB7 cell MX\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NIrvZZZKdmirYnn0bY9vKG:oIHj1cYFvKE6ENzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuO|UzQTdizszN NWe2RYFuW0GQR1XS
human LAMA-84 cell M3fmZ2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVfKTVN6UW6qaXLpeIlwdiCxZjDoeY1idiCOQV3BMVg1KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS55NUWyJO69VQ>? NU\VT4Y6W0GQR1XS
human HCT-116 cells NIX1XnpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M{HN[GlvcGmkaYTpc44hd2ZiaIXtZY4hUEOWLUGxOkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvQDB7MEig{txO Ml3VV2FPT0WU
SK-UT-1 cell MlfPS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUWxUndRUW6qaXLpeIlwdiCxZjDoeY1idiCVSz3VWE0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS56MUWzJO69VQ>? NGrpfXdUSU6JRWK=
human H4 cell MmjOS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MX\Jcohq[mm2aX;uJI9nKGi3bXHuJGg1KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS56MUW3PEDPxE1? NHvvOplUSU6JRWK=
human CAL-51 cell M1XXfGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MUDJcohq[mm2aX;uJI9nKGi3bXHuJGNCVC13MTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuPFM5PDVizszN M4jVfHNCVkeHUh?=
human LoVo cells MmLtVJJwdGmoZYLheIlwdiCjc4PhfS=> NV[3W45MPzJiaB?= NH\SfFRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFzvWo8h[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWE1j[XOnZDDXV3Q5KHKnYXflcpQh[XO|YYmsJGlEPTB;MT65JO69VQ>? MW[yOVI4ODRyMx?=
human HN cell NV\GVmZOT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXHJcohq[mm2aX;uJI9nKGi3bXHuJGhPKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS57MkWxJO69VQ>? NIq0VI5USU6JRWK=
human L-363 cell M{TmU2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Ml30TY5pcWKrdHnvckBw\iCqdX3hckBNNTN4MzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuPVUyOiEQvF2= MYLTRW5ITVJ?
human NCI-H747 cell NUnOb5g3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUe5UXFCUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFc1PyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTJwMEOzOVMh|ryP NWfD[IpGW0GQR1XS
human A498 cell M2\y[Gdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4PtRmlvcGmkaYTpc44hd2ZiaIXtZY4hSTR7ODDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKuN|Y3QSEQvF2= Mke3V2FPT0WU

... Click to View More Cell Line Experimental Data

Protocol

Kinase Assay:[1]
+ Expand

In vitro kinase assays :

Recombinant Aurora A and B are expressed as NH2-terminal His6-tagged fusion proteins using a baculovirus expression system. Aurora A is purified by affinity chromatography using Ni-NTA agarose, and Aurora B is purified by ion exchange chromatography using CM Sepharose Fast Flow. 1 ng purified recombinant enzyme is added to a reaction cocktail containing 25 mM Tris-HCl, pH 7.5, 12.5 mM KCl, 2.5 mM NaF, 0.6 mM DTT, 6.25 mM MnCl2, 10 μM peptide substrate, 10 μM for Aurora A or 5 μM ATP for Aurora B, and 0.2 μCi γ-[33P]ATP (specific activity ≥2,500 Ci/mmol), and is then incubated at RT for 60 minutes. Reactions are stopped by addition of 20% phosphoric acid, and the products are captured on P30 nitrocellulose filters and assayed for incorporation of 33P with a BetaplateTM counter. No enzyme and no compound control values are used to determine the concentration of ZM447439, which gave 50% inhibition of enzyme activity. Further details are available on request from Nicholas Keen.
Cell Research:[2]
+ Expand
  • Cell lines: BON, QGP-1 and MIP-101 cells
  • Concentrations: 0-5 μM
  • Incubation Time: 72 hours
  • Method: Cell number is evaluated by crystal violet staining. In brief, cells in 96-well plates are fixed with 1% glutaraldehyde. Then cells are stained with 0.1% crystal violet. The unbound dye is removed by washing with water. Bound crystal violet is solubilized with 0.2% Triton X-100. Light extinction which increases linearly with the cell number is analyzed at 570 nm using an ELISA reader.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 103 mg/mL (200.54 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents individually and in order:
30% PEG400+0.5% Tween80+5% propylene glycol
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 513.59
Formula

C29H31N5O4

CAS No. 331771-20-1
Storage powder
Synonyms N/A

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Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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