Rapamycin (Sirolimus) Licensed by Pfizer

Rapamycin (Sirolimus) is a specific mTOR inhibitor with IC50 of ~0.1 nM HEK293 cells.

Price Stock Quantity  
In DMSO USD 306 In stock
USD 70 In stock
USD 270 In stock
USD 670 In stock
Bulk Inquiry

Massive Discount Available

Free Overnight Delivery on all orders over $ 500.

Rapamycin (Sirolimus) Chemical Structure

Rapamycin (Sirolimus) Chemical Structure
Molecular Weight: 914.18

Validation & Quality Control

Product Use Citation(54)

Customer Product Validation(12)

Quality Control & MSDS

Related Compound Libraries

mTOR Inhibitors with Unique Features

  • Pan mTOR inhibitor

    KU-0063794 mTORC1 and mTORC2, IC50=~10 nM.

  • FDA-approved mTOR Inhibitor

    Temsirolimus (CCI-779, NSC 683864) Approved by FDA for Renal Cell Carcinoma (RCC).

  • Newest mTOR Inhibitor

    XL388 Highly potent, selective, ATP-competitive inhibitor of mTOR with IC50 of 9.9 nM, 1000-fold selectivity over the closely related PI3K kinases.

  • Classic mTOR Inhibitor

    BEZ235 (NVP-BEZ235, Dactolisib) Dual ATP-competitive PI3K and mTOR inhibitor for p110α/γ/δ/β and mTOR(p70S6K) with IC50 of 4 nM/5 nM/7 nM/75 nM/6 nM, respectively.

Product Information

  • Compare mTOR Inhibitors
    Compare mTOR Products
  • Research Area
  • Combination Therapy
    Combination Therapy

Product Description

Biological Activity

Description Rapamycin (Sirolimus) is a specific mTOR inhibitor with IC50 of ~0.1 nM HEK293 cells.
Targets mTOR [1]
(HEK293 cells)
IC50 ~0.1 nM
In vitro Rapamycin inhibits endogenous mTOR activity in HEK293 cells with IC50 of ~0.1 nM, more potently than iRap and AP21967 with IC50 of ~5 nM and ~10 nM, respectively. [1] In Saccharomyces cerevisiae, Rapamycin treatment induces a severe G1/S cell cycle arrest and inhibition of translation initiation to levels below 20% of control. [2] Rapamycin significantly inhibits the cell viability of T98G and U87-MG in a dose-dependent manner with IC50 of 2 nM and 1 μM, respectively, while displaying little activity against U373-MG cells with IC50 of >25 μM despite the similar extent of the inhibition of mTOR signaling. Rapamycin (100 nM) induces G1 arrest and autophagy but not apoptosis in Rapamycin-sensitive U87-MG and T98G cells by inhibiting the function of mTOR. [3]
Cell Data
Cell LinesAssay TypeConcentrationIncubation TimeFormulationActivity Description
HT-29MmPKR5l1d3SxeHnjJGF{e2G7M1zMN|ExKG6PM{\ZfFczKGh?M3;KSGROW09?NYTaS|k2WG:2ZX70bYF1\XNiY3HtdJRwfGinY3nuMYlv\HWlZXSgZ5l1d3SxeHnjbZR6
HT-29NXniVmJLS3m2b4TvfIlkKEG|c3H5MYKxNEBvVQ>?M{TtSFczKGh?NWfDO5lnTE2VTx?=NVP1WVBrWG:2ZX70bYF1\XNiZHnnbZRwgGmwLXnu[JVk\WRiY4n0c5RwgGmlaYT5
HT-29MW\DfZRwfG:6aXOgRZN{[Xl?MWSxNEBvVQ>?NFS1ZXk4OiCqNXnFUIg1TE2VTx?=M{jHOXBwfGWwdHnheIV{KDVvZnz1c5JwfXKjY3nsMYlv\HWlZXSgZ5l1d3SxeHnjbZR6
PC3MlywT4lv[XOnIFHzd4F6MmXNNVAxKG6PMlXuNUBpNXnmWFdkTE2VTx?=M4HTcXBwfGWwdHz5JIlvcGmkaYTzJI1VV1JvbXXkbYF1\WRiU{[gdIhwe3Cqb4L5cIF1cW:wIIfpeIghUUN3MDDv[kA9OTBibl2u
PC3NFfMN5NMcW6jc3WgRZN{[Xl?MXyxNFAhdk1?NHLCUGsyKGh?NIPHRlBFVVORNWHkSG9DTG:nczDuc5QhcW6qaXLpeEBuXE:ULX3l[IlifGWmIFHreEBxcG:|cHjvdplt[XSrb36=
PC3Ml;tS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MkDDNU42KM7:TR?=MoTWNUBpM2L1U2ROW09?MkHDTY5lfWOnczDhcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFCFMzDj[YxteyC5aYToJGlEPTBib3[gQFExKG6P
HEK293MXvGeY5kfGmxbjDBd5NigQ>?Mk\LNVAxKG6PNYHTO3VrQCCqMn3XSG1UVw>?NITrN3hKdmirYnn0d{BVWEFvaX7keYNm\CCmZXfyZYRifGmxbjDv[kBR\GOmNDD3bZRpKEWFNUCgc4YhPTBibl2=
BT-20MW\LbY5ie2ViQYPzZZk>NEixZZYzOCEQvF2=M4noPGROW09?NXzMXm97TG:nczDuc5QhcW6qaXLpeEBuXE:UQ{Kg[IVx\W6mZX70JJBCc1RiU{S3N{BxcG:|cHjvdplt[XSrb36=
U937M1rOU2FvfGmkYXP0[ZJq[WxiQYPzZZk>MkfuOVAh|ryPMmH3OFghcA>?MV3EUXNQMojPTY5lfWOnczDhcpRq[mGldHXybYFtKGGldHn2bZR6KGGpYXnud5Qhf2muZDD0fZBmKEynZ3nvcoVtdGFicH7leY1weGirbHGgVIhqdGGmZXzwbIliNTFiSmKzNkBqdiCXOUO3JINmdGy|
U937MkO4RY51cWKjY4TldolidCCDc4PhfS=>NUn6XGRSPTBizszNM3\1cFQ5KGh?NWXtWlZUTE2VTx?=M1HzWWRw\XNibn;0JIlv\HWlZTDhcpRq[mGldHXybYFtKGGldHn2bZR6KGGpYXnud5QhVUmSIIDyc5RmcW5vZHXmbYNq\W62IFzl[4lwdmWubHGgdI5mfW2xcHjpcIEhUlJ|Mj2yJIlvKFV7M{egZ4VtdHN?
U937M1vIbWFvfGmkYXP0[ZJq[WxiQYPzZZk>MnfHOVAh|ryPM2X1W|Q5KGh?NEDMZmRFVVORMXnEc4V{KG6xdDDpcoR2[2ViYX70bYJi[3SncnnhcEBi[3Srdnn0fUBi\2GrboP0JGV{[2incnnjbIliKGOxbHmgTGIyODFiaX6gWVk{PyClZXzsdy=>
MCF-7NWnJUpNNSXW2b4DoZYd6KEG|c3H5NYjrXFFCOzBibl2=NFLpdIQ1KGh?MYfEUXNQMUnJcoR2[2W|IHH1eI9xcGGpeR?=
U87MGMWTLbY5ie2ViQYPzZZk>M{HoWVEh|ryPMVy2JIg>M1;4NmROW09?M4SxWnBwfGWwdHz5JIlvcGmkaYTzJI1VV1JvbXXkbYF1\WRiU{[gdIhwe3Cqb4L5cIF1cW:w
U87MGMX;LbY5ie2ViQYPzZZk>NWPwNWpuOSEQvF2=MVK2JIg>M2D6R2ROW09?MV;Qc5RmdnSueTDpcohq[mm2czC0SWJROSiWN{CpJJBpd3OyaH;yfYxifGmxbh?=
U87MGMmTJT4lv[XOnIFHzd4F6M1;0flEh|ryPM2PmblYhcA>?M3y5SGROW09?M1m3OGRw\XNibn;0JIlvcGmkaYSgcXRQWi2vZXTpZZRm\CCDa4SgdIhwe3Cqb4L5cIF1cW:w
U87MGMmTDT4lv[XOnIFHzd4F6M1\lSlEh|ryPM2O2WlYhcA>?MofVSG1UVw>?NWjjRXhzTG:nczDuc5QhcW6qaXLpeEBRNTSHQmCxLHQ{Py92NjmgdIhwe3Cqb4L5cIF1cW:w
COS7 cells expressing EGFP-HDQ74/rhebMV;BeZRweGijZ4mgRZN{[Xl?NGXFWHUxNjJizszNM2\CNVI1KGh?MofoSG1UVw>?Mk\aTY5lfWOnczDheZRweGijZ4m=
COS7 cells expressing EGFP-LC3MmLURZV1d3CqYXf5JGF{e2G7NInxTY4xNjJizszNNXXPWY91OjRiaB?=MonvSG1UVw>?NFPv[2hKdmS3Y3XzJIF2fG:yaHHnfS=>
H4NYjSdYR[TnWwY4Tpc44hSXO|YYm=M1WzWVAvOiEQvF2=MoXUNlQhcA>?NUXWTo9nTE2VTx?=NE\nSWRKdmO{ZXHz[ZMhfGinIILheIlwKG:oIHzp[4h1KGOqYXnuJFMhe3WkdX7peEAzKHSxIHzp[4h1KGOqYXnuJFMhe3WkdX7peEAyKGmwIHj1cYFvKEh2IHPlcIx{
HeLaM4TJRmZ2dmO2aX;uJGF{e2G7MUGxNFAhdk1?MmrqN|YhcA>?NEXKbZVFVVORM3TtUmlv\HWlZYOgSnJDKEt{MEm1VEwhXDJyOUjMMEBYOjFyMV[gcZV1[W62LYXibZF2cXSrblOgbY51\XKjY4Tpc44>
HeLaMnjCSpVv[3Srb36gRZN{[Xl?MWOxNFAhdk1?MnPIN|YhcA>?M3;ZeGROW09?NV3Sem9wUW6mdXPld{BHWkJiV{KxNFFHKG23dHHueE12[mmzdXn0bY5EKGmwdHXyZYN1cW:w
HeLaNWK3WWY6TnWwY4Tpc44hSXO|YYm=MXKxNFAhdk1?MXWzOkBpMnGzSG1UVw>?MoHCTY5lfWOnczDGVmIuTkuEUDDjc41xdGW6IHnueIVz[WO2aX;u
SYFNVXXdGJoTnWwY4Tpc44hSXO|YYm=NYjZZpp4OTByIH7NM1ntelI1KGh?Mnf4SG1UVw>?NHPTWFdKdmS3Y3XzJGZTSi2IS1LQJINwdXCuZYigbY51\XKjY4Tpc44>
SYFNW\3bZRMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=M3vo[FExOCCwTR?=MnLBNlQhcA>?MlfmSG1UVw>?NH3HUGRKdmS3Y3XzJIFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgV3lHKGOnbHzz
HEK293TMX\BcpRqfmm{YXygRZN{[Xl?MnL2NUBvVQ>?M1fQW|Qh\A>?M1fEeGROW09?NYfS[IZEUW6mdXPld{BidnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIGK1JJdqfGhiRVO1NEBw\iByLkGgcm0>
HEK293TM3jZZ2FvfGm4aYLhcEBCe3OjeR?=NUfs[mtFOSCwTR?=NXrRWlh5PCCmM1fRNmROW09?NWrrfXRpUW6mdXPld{BidnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIGi0JJdqfGhiRVO1NEBw\iByLkOgcm0>
PBMCNHHrRplHfW6ldHnvckBCe3OjeR?=M{LKW|Ehdk1?M2rad|E1KGR?NEn0UFRFVVORM1XYfHJm\HWlZYOgR2NTPSCmZX7zbZR6
PBMCMYPGeY5kfGmxbjDBd5NigQ>?NGrEXXIyKG6PMWWxOEBlNIPFO4pFVVORNVqze4ZKTG:nczDuc5Qh[W[oZXP0JGNZS1J2IHTlcpNqfHl?
HEK293 cellsMoTtT4lv[XOnIFHzd4F6MVG1NEBvVQ>?NWD4fWFUPDVibXnuM2[zfGROW09?MkO2TY5pcWKrdIOgcXRQWiCtaX7hd4Uh[WO2aY\peJkhf2m2aDDJR|UxKG:oIECuNUBvVQ>?
Drosophila melanogaster S2 cells transfected with N-luc and C-lucNY\UV|dyTnWwY4Tpc44hSXO|YYm=NYLVdFZ3OTByIH7NNH73ZpE1KGh?NEnnOXdFVVORNHTjdpdKdmS3Y3XzJIx2[2moZYLhd4UheHKxdHXpckB1emGwcz3zdIxq[2mwZzDpckBFem:|b4DobYxiKG2nbHHuc4die3SncjDTNkBk\WyuczD0doFve2[nY4Tl[EB4cXSqIF6tcJVkKGGwZDDDMYx2[w>?
Human mixed lymphocyteNXLhbJQ2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MXG1JI5ONFTMTIdFVVORMV3JR|UxRTFwNjDuUU4>
Lewis rat lymph node cellsM2WydWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NGDwZ3g2KM7:TR?=NUezbmo3TE2VTx?=NIfS[oVKSzVyPUKuOkDPxE1?
cells from the thymus of normal BALB/c miceMn;hS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M3LkU|ExKG6PMYS3NkBpMlTNSG1UVw>?M{fpb2lvcGmkaYTzJIx6dXCqb4Dyc4xq\mW{YYTpc44hMEyDRjmge4l1cCCLQ{WwJI9nKDNibl2=
MRK-nu-1NGLO[o1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NVjaS25nUUN3ME2wMlg1PSCyTR?=
OCUB-MNGrkfZlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NEWx[GNKSzVyPUWuNlQheE1?
SF539M4Lafmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MWnJR|UxRTFzLk[gdG0>
ES4MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MX;JR|UxRTJzLkWgdG0>
RL95-2NEjNdmtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NHLpW4ZKSzVyPUGwO{BxVQ>?
LC-2-adNEX0VphIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MkfsTWM2OD12MkOgdG0>
DaudiNETZPYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NV\zeYxOUUN3ME20N|QheE1?
NTERA-S-cl-D1M2\uO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7MkTYTWM2OD12NEOgdG0>
OS-RC-2Mo\1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MWTJR|UxRTZ3MjDwUS=>
VA-ES-BJMVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NGfMWlBKSzVyPUeyN{BxVQ>?
GR-STNIfy[lNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=Mom2TWM2OD16NE[gdG0>
SW872MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MV7JR|UxRTh2NjDwUS=>
NOS-1M1ryeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7M3LpWWlEPTB;OEexJJBO
MC116MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NFjGR2lKSzVyPUm4OUBxVQ>?
NCI-H1355NWTNfm8xT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=M2\WNWlEPTB;MT6wNUBvVQ>?
RPMI-8226NVOwS5lNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MnfhTWM2OD1zLkG5JI5O
TE-15M4D4OWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NYLUSGZbUUN3ME2xMlM3KG6P
Ramos-2G6-4C10M1vqVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NULpN2VoUUN3ME2xMlQ3KG6P
KU812M{\GXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NUHGTGxmUUN3ME2yMlAyKG6P
EW-1Ml3IS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NY\xUWk1UUN3ME2yMlE4KG6P
KS-1MnvRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MmX5TWM2OD1{LkS1JI5O
SK-LMS-1MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NXXPcXFxUUN3ME2yMlQ6KG6P
TGBC1TKBNVHTeIk1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NUX5bXUyUUN3ME2yMlY6KG6P
TE-6M{nVb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7MWfJR|UxRTJwN{egcm0>
ETK-1M1r1OWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MmXLTWM2OD1{LkiyJI5O
BE-13NWPWTnhGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MofETWM2OD1{Lkm5JI5O
A3-KAWM3zkeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MVLJR|UxRTJwOUmgcm0>
TE-10M3v5VGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MWPJR|UxRTNwMzDuUS=>
DOHH-2M{\XUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NF2xW2tKSzVyPUOuN|Uhdk1?
ES6Ml;US5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MmT6TWM2OD1|LkSzJI5O
OPM-2NXnpeG1oT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=M3TEXGlEPTB;ND6xOUBvVQ>?
SH-4MmC5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MXrJR|UxRTRwM{Sgcm0>
NB13NVjGcnV{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NEnLeYVKSzVyPUSuN|Yhdk1?
HUTU-80MkfuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MUnJR|UxRTRwNEKgcm0>
CCRF-CEMM{i2cWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NX7C[JhKUUN3ME20Mlk1KG6P
TGBC24TKBMkTUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NXPMT3JbUUN3ME21MlUyKG6P
697NWm4W|Z7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MoLkTWM2OD14LkK4JI5O
J-RT3-T3-5MnGwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NH73PFJKSzVyPU[uOFYhdk1?
KALS-1MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NVrQbI1IUUN3ME22MlU3KG6P
no-10NFvPRoNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NWf1PIN{UUN3ME23MlI6KG6P
SK-NEP-1Mo\kS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M2[0[WlEPTB;OD63PUBvVQ>?
L-540NGPRfI1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MV;JR|UxRTFyLkSyJI5O
JiyoyeP-2003M3fZb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7MUPJR|UxRTFyLkm0JI5O
HHNYnQUoFMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=M3;PVGlEPTB;MUGuN|khdk1?
SRNH64WIxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MVzJR|UxRTFzLkS1JI5O
QIMR-WILMnXIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NY\ocXp5UUN3ME2xNU45PSCwTR?=
A4-FukM372Smdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NVW0XW5pUUN3ME2xN{4yOiCwTR?=
CESSNWDobWpuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NIH2U2JKSzVyPUGzMlE{KG6P
KE-37Ml;lS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MU\JR|UxRTF4LkC3JI5O
SK-UT-1M{HIOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NFXqW2JKSzVyPUG2MlgyKG6P
SIG-M5M3y5[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MV3JR|UxRTF5LkK1JI5O
HTMkHqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MVXJR|UxRTF5Lk[gcm0>
DELMVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NWjEUVZvUUN3ME2xO{46QSCwTR?=
SK-PN-DWNUjr[I1KT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=M4H2VWlEPTB;MkCuNlMhdk1?
RPMI-8402Mn\wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NFrnUFhKSzVyPUKxMlc4KG6P
RPMI-6666NVXnUWZlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=Mk\wTWM2OD1{ND60NkBvVQ>?
NCI-H720NUXxd2FKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=M3SwN2lEPTB;MkWuOFEhdk1?
EW-16M2H5bGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MkTRTWM2OD1{Nj64O{BvVQ>?
BL-70NWj6OHJQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=M3u0ZWlEPTB;MkiuN|ghdk1?
SF126NFHPNlJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MY\JR|UxRTNyLkO4JI5O
BC-1M1jBRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7Ml;LTWM2OD1|MT6yOkBvVQ>?
MHH-PREB-1NYXuVXc3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MV\JR|UxRTN{LkS0JI5O
A101DNUnUVXVwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=Mo\ITWM2OD1|Mj62NkBvVQ>?
NMC-G1MnzzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MlOyTWM2OD1|Mz62O{BvVQ>?
LB1047-RCCMYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M1PXPGlEPTB;M{SuOlkhdk1?
EM-2MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?Mm\zTWM2OD1|OD61N{BvVQ>?
COLO-684NXfWVXE6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NGnlcYpKSzVyPUO5Mlghdk1?
BeckerM4TsOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MmfzTWM2OD12MT6wOUBvVQ>?
BL-41MonuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MlPkTWM2OD12Mz62OkBvVQ>?
MDA-MB-134-VIMX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MVTJR|UxRTR2LkCyJI5O
L-363NUKyfoJFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MUTJR|UxRTR2LkezJI5O
ECC4MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NYDzdmhFUUN3ME20OE44QCCwTR?=
A388MmjQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MV\JR|UxRTR2LkiyJI5O
HELNE[1bmtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=M2L0NGlEPTB;NEmuO|khdk1?
RKOMXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M1uwdGlEPTB;NUCuNlkhdk1?
KINGS-1MnvRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NILMSWZKSzVyPUWxMlU2KG6P
EB-3M1TVdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MYLJR|UxRTV{Lk[3JI5O
ARH-77MkO5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MlzxTWM2OD13Mj64JI5O
GCIYNFLmZmtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NVnoOWh6UUN3ME21N{41PiCwTR?=
NCI-H1304MnXyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NH3OWVdKSzVyPUW3MlIzKG6P
KARPAS-299NH7ocYNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=Mo\uTWM2OD14MT64NkBvVQ>?
IA-LMNYC0dno1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MonBTWM2OD14OD6xN{BvVQ>?
GI-1MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NI\telVKSzVyPUewMlM6KG6P
TE-11NH3ZenNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NXX5WJM5UUN3ME23O{4yPyCwTR?=
LS-411NNVzY[pVOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MVPJR|UxRTd5LkW3JI5O
no-11NEP3TIdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MXHJR|UxRTh|LkK0JI5O
MV-4-11MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MY\JR|UxRTh|LkezJI5O
BV-173Ml3hS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M1vFfWlEPTB;OEOuPVchdk1?
CMKM{Sxb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7NVvoR3hLUUN3ME24OE4yPiCwTR?=
LC4-1Ml;kS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MlXZTWM2OD16Nj63NkBvVQ>?
COR-L279M3fPTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MWnJR|UxRTh5LkK1JI5O
NCI-H209MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NV;QfmllUUN3ME24O{41OSCwTR?=
RajiMXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MlHtTWM2OD16OT63NkBvVQ>?
LB996-RCCNVHiZoJ2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=Mn2xTWM2OD17Mz60N{BvVQ>?
NCI-H526MnfjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?Ml;jTWM2OD17Mz61PUBvVQ>?
KGNMmC2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M{P2TmlEPTB;OU[uNlkhdk1?
MOLT-4NHnUSZlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NX7JUpltUUN3ME25Ok44QSCwTR?=
PF-382NXi4eGlFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NV\2RohuUUN3ME25Ok44QSCwTR?=
BC-3NF\DSmlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NFThem5KSzVyPUm5MlE5KG6P
KARPAS-422NXfieHFjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=Mm\3TWM2OD1zMEKuNFkhdk1?
SBC-1MmX1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?Ml[0TWM2OD1zMEeuO|Uhdk1?
LC-1FMn3QS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MXvJR|UxRTFyOD6wOUBvVQ>?
GB-1NGXVSndIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NFnBWW1KSzVyPUGwPU4xOiCwTR?=
SNB75M1joZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NH;qSZBKSzVyPUGxPU43QSCwTR?=
BB65-RCCM1\0PWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NFX4NHpKSzVyPUGxPU46OyCwTR?=
NCI-N87M3nq[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7M4XYUGlEPTB;MUKxMlk5KG6P
IST-MEL1MnHSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MYLJR|UxRTF{Mj6zPEBvVQ>?
HOP-62NWmyUpJRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=M3zLNGlEPTB;MUK2Mlg6KG6P
ACNNVPWS4V[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MkKyTWM2OD1zNE[uO|Uhdk1?
DMS-114NHfxOVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NUnoNmJXUUN3ME2xOVAvPjdibl2=
MLMAMlTRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NIC1SllKSzVyPUG1PU45QCCwTR?=
HT-144NV\HSVM1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NIX3eHFKSzVyPUG2OU41OyCwTR?=
C2BBe1NH:yZVlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=M4HycGlEPTB;MU[3Mlc3KG6P
L-428MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NHS0S|VKSzVyPUG3O{44KG6P
DU-4475NUTzXGo3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NVvu[VNkUUN3ME2xPFcvPjhibl2=
CP67-MELNIjJVpZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NFm4SHZKSzVyPUG5PU4{QCCwTR?=
MEG-01NFPIXHRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MUHJR|UxRTJyMT65OkBvVQ>?
IST-SL2MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NHLlWJRKSzVyPUKwPE43OyCwTR?=
ES8NFnIS5VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NXXlW2VoUUN3ME2yNlUvQTRibl2=
COLO-800MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M1LwOmlEPTB;MkO1MlI5KG6P
MFH-inoNV2ybI5wT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NYW3e4VGUUN3ME2yN|UvQDRibl2=
OVCAR-4M1:xXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NH\JeJBKSzVyPUKzO{4zPCCwTR?=
PSN1MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NEW0NlBKSzVyPUK0Nk44OSCwTR?=
EW-12NVvLc|ZTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MUfJR|UxRTJ2Mz6xJI5O
HCC1599M4DOPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7M4rURmlEPTB;Mk[xMlQ4KG6P
SJSA-1NUDSVGtbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MXnJR|UxRTJ5MT60OkBvVQ>?
ST486NF\qWpBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MXvJR|UxRTJ7Nj6xOEBvVQ>?
NOMO-1NXWyVVNKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NHq1enZKSzVyPUOwNE4zOSCwTR?=
MN-60MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NY\jOHFoUUN3ME2zNFUvOzJibl2=
HCC1187NGrNSZBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MmrDTWM2OD1|MEeuNlUhdk1?
SW982M4TKWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MYHJR|UxRTNzND63OUBvVQ>?
LB647-SCLCM3roeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7M2fZUmlEPTB;M{K4MlcyKG6P
HC-1NVXLTVRGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=M2rWZWlEPTB;M{O1MlUhdk1?
EHEBMkfES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MWjJR|UxRTN|Nz61NkBvVQ>?
TURM4HRdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MVLJR|UxRTN4Mz65OUBvVQ>?
LU-139NEXLO4NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=M{XFfGlEPTB;M{e4MlAzKG6P
NB1MnfuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MWnJR|UxRTN6ND60OUBvVQ>?
BB30-HNCNIXNWWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NH;GeZFKSzVyPUO4PE4{OiCwTR?=
HAL-01MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MkTxTWM2OD1|OEmuNlYhdk1?
K5NVnJe2tGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NVPtUJVLUUN3ME20NVEvOzdibl2=
MZ2-MELMUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NY\SWmpEUUN3ME20NVMvPjRibl2=
RXF393NH;KRnRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NGTESpdKSzVyPUSxOk41PSCwTR?=
NCI-H1648MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M{XOU2lEPTB;NEG3MlU{KG6P
TE-12MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NX[5foxEUUN3ME20N|QvOjZibl2=
EoL-1-M{XKdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MnnsTWM2OD12M{euPVghdk1?
JARNI\JboFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MoC1TWM2OD12M{iuOlIhdk1?
DSH1NHPPT49Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MoTZTWM2OD12NUiuPVEhdk1?
NCI-H187MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NYDjfnoyUUN3ME20OlIvQDFibl2=
HCE-4NXXXZ2c6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MkfITWM2OD12N{euOlYhdk1?
8-MG-BANWmwNph6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=M{e4c2lEPTB;NUixMlUzKG6P
KLENYTE[mI2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NIDOfGFKSzVyPUW4OU4zKG6P
KNS-42MnTlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NHf5TIRKSzVyPUW4Ok45OSCwTR?=
MSTO-211HNVGyeVNMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NWPMfYRbUUN3ME22NFkvPzRibl2=
GDM-1MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M1vsd2lEPTB;NkG0MlA6KG6P
TE-1MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MYjJR|UxRTZ2Nj6xNkBvVQ>?
BT-474NInjTmdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NYHTSnZZUUN3ME22OFcvODZibl2=
KARPAS-45NHi1OYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NVPrcYdMUUN3ME22OFcvPiCwTR?=
MOLT-16M3fzZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NHW2N3RKSzVyPU[0O{46OyCwTR?=
KURAMOCHINXz2OpM4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NGjOWG5KSzVyPU[1O{42OSCwTR?=
K-562MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M37uWmlEPTB;Nk[5MlUyKG6P
EKVXMoroS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NWX6dpk6UUN3ME22O|IvPzFibl2=
GAKMXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?Mly2TWM2OD14N{WuN{BvVQ>?
NCI-SNU-5NGnTU3lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=M3PJOWlEPTB;NkmwMlAyKG6P
NCI-H2126M2XrZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NWT6NYVbUUN3ME23NlYvQDdibl2=
CTV-1NXmxXmFNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NIHFfJVKSzVyPUe0OE46KG6P
SW962NGrZWmhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NV61flZIUUN3ME23OFgvPDRibl2=
MONO-MAC-6NUD3UmxPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MXjJR|UxRTd3Nj65N{BvVQ>?
NCI-H748NVW5cXpET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MVvJR|UxRTd3OD65PUBvVQ>?
NCI-H524MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MnfBTWM2OD15OECuO|Mhdk1?
LS-123NFT6fpVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MlvhTWM2OD15OUWuOlkhdk1?
NB7NYDY[3BNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MWPJR|UxRThzND6xOEBvVQ>?
LS-1034M3XWWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NUnV[ndzUUN3ME24NlgvQThibl2=
TE-5NGriXplIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MVfJR|UxRTh6Mz61OkBvVQ>?
A704MnjnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MVjJR|UxRTh7OT6xOUBvVQ>?
TK10M4D5RWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NIfPcFJKSzVyPUmxOk4xOyCwTR?=
NCI-H345NYflZ2w1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MYDJR|UxRTl2Mz6yNkBvVQ>?
CGTH-W-1MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NUPhVIIyUUN3ME25OFgvOTNibl2=
NCI-H510AMmTFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NEfNRWdKSzVyPUm4OU4yOiCwTR?=
NCI-H1963NF\QVmlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=M165VGlEPTB;MT6wN|I6OiEQvF2=
SCC-3NELGWVJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NYLSeXZzUUN3ME2xMlA{PDF2IN88US=>
EW-11NWrxcWJxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MVHJR|UxRTFwMEi3OFMh|ryP
CPC-NNG\iWWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MoDOTWM2OD1zLkC4PEDPxE1?
NCI-H1417M3TNbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MUHJR|UxRTFwMUKyOkDPxE1?
DG-75MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NH7Q[5lKSzVyPUGuNVYzQDVizszN
HD-MY-ZNF;jNlRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NX;HPYxnUUN3ME2xMlE3PDF4IN88US=>
ATN-1MoLZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?Mne3TWM2OD1zLkK2NlA6KM7:TR?=
KM-H2NUH3PYtnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NEGxcVhKSzVyPUGuNlY1ODhizszN
NCI-H2081NHjhcJNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NWLnTGZUUUN3ME2xMlI3PjN5IN88US=>
HL-60MlXGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NH22O3VKSzVyPUGuNlY6PTlizszN
DBNXnPW4s4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MWPJR|UxRTFwMkeyOFIh|ryP
NCI-H1522MkPOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M{jO[2lEPTB;MT6yPFg5PyEQvF2=
AM-38MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M4PJVGlEPTB;MT6zNFczKM7:TR?=
NCI-H446NG\MTJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MUnJR|UxRTFwM{KxNlEh|ryP
SU-DHL-1M2e2PGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NEjUfolKSzVyPUGuN|I5ODFizszN
NH-12MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MXTJR|UxRTFwM{[zO|Qh|ryP
DMS-79MoXOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M{\iW2lEPTB;MT6zOlg3PiEQvF2=
NCI-H716NYfQUIZXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=M4fPNWlEPTB;MT6zPFk5PiEQvF2=
ML-2NE\G[FBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=M4HhVWlEPTB;MT60NVUzQSEQvF2=
NB10NXnZblgzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MnzoTWM2OD1zLkS2OlMzKM7:TR?=
ONS-76MmXTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M{HKdmlEPTB;MT61N|U3QSEQvF2=
LOUCYMW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MlfXTWM2OD1zLkW0OlU4KM7:TR?=
SCLC-21HM1rMbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7M2LNRmlEPTB;MT61PFU5OiEQvF2=
TGWMmfES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MnvvTWM2OD1zLk[zPVc2KM7:TR?=
LXF-289NVvFeY5TT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=M1PSdmlEPTB;MT63N|I3QCEQvF2=
BB49-HNCMnXSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MlLqTWM2OD1zLkezOVg3KM7:TR?=
NCI-H747MoGwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?Mk\UTWM2OD1zLke1N|Q3KM7:TR?=
LU-165NEDJUGxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MYTJR|UxRTFwOES5PFYh|ryP
OMC-1NUXjPXVtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MoSwTWM2OD1zLkm1NFY3KM7:TR?=
RCC10RGBMULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M1jmdGlEPTB;MT65OVgyPyEQvF2=
SW684NWqxUXFOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MVLJR|UxRTFwOU[wPVkh|ryP
TE-8MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MlXlTWM2OD1{LkC1OVU6KM7:TR?=
SK-N-DZNHjiOlRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MmDHTWM2OD1{LkGzNlc1KM7:TR?=
EVSA-TNXXaelJXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MXTJR|UxRTJwMUezNVUh|ryP
KASUMI-1MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NXK1UZQ3UUN3ME2yMlE5QDF3IN88US=>
NKM-1M4rVO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7M{SyTWlEPTB;Mj6yOVQ4OiEQvF2=
CAL-148MkLOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MoroTWM2OD1{LkOzOlE1KM7:TR?=
NCI-H64NYW5eItNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NVvTfIllUUN3ME2yMlM1OjN{IN88US=>
KNS-81-FDM2e5Z2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7NVvLenpZUUN3ME2yMlM3PjJizszN
KM12NXfMVVU{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MV7JR|UxRTJwNEC4N|kh|ryP
SW954NFjQOm9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=M2\ZUGlEPTB;Mj60O|c4QSEQvF2=
NCI-H1395MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NU\XTYlIUUN3ME2yMlUzPjR3IN88US=>
DJM-1Mnq4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NVPKOVl7UUN3ME2yMlYxPjNizszN
COLO-668M4\6[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MoXiTWM2OD1{LkiyOlk2KM7:TR?=
NCI-H1436MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MXTJR|UxRTJwOEW2NVUh|ryP
LB2241-RCCMXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M1PzTWlEPTB;Mj64Olg{QSEQvF2=
GT3TKBM4TxWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NVPDR|VxUUN3ME2yMlg6ODV3IN88US=>
COLO-824NFvDbZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NXv2ZphuUUN3ME2yMlg6PzZ6IN88US=>
ES1NUnJWZJuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NWTrUlM6UUN3ME2yMlg6QDd7IN88US=>
LB771-HNCNWHrNYQ6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=M4LrRWlEPTB;Mj65NFk1PiEQvF2=
GI-ME-NMnHaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MYLJR|UxRTNwMEC5NFQh|ryP
NALM-6MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NV7DPXJjUUN3ME2zMlAxQTN|IN88US=>
LU-134-AMl7JS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MWHJR|UxRTNwMEW0NlUh|ryP
DMS-153MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?Moj3TWM2OD1|LkC1PFI1KM7:TR?=
MZ1-PCNIXPNFhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NVHFXlF5UUN3ME2zMlA6ODd6IN88US=>
NCI-H1155NFjPT41Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MlzpTWM2OD1|LkGxOlEh|ryP
CAS-1MoGzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M3n1W2lEPTB;Mz6xN|cxPyEQvF2=
D-502MGNWT6RlViT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NGe0eJdKSzVyPUOuNVQ{QSEQvF2=
NCI-H2141Mlz4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NFPsd2JKSzVyPUOuNVc1PTJizszN
NB6MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M1O3NmlEPTB;Mz6xPFI2QSEQvF2=
NCCITM1HmbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7M4HwN2lEPTB;Mz6yNVgxQSEQvF2=
NB69NIL6cpVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NXTaN4xIUUN3ME2zMlMyQDlzIN88US=>
JVM-2MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NHXRWXpKSzVyPUOuN|Y1OzNizszN
K052NFTRfIVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MVTJR|UxRTNwM{e5Olgh|ryP
HCC2157MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MV7JR|UxRTNwNUOyNlgh|ryP
KMOE-2NWnkR3VFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=M2e3UWlEPTB;Mz61OFI1OiEQvF2=
SF268M2\H[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NHzwW|FKSzVyPUOuO|E2PTRizszN
CHP-126MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NEfBXJRKSzVyPUOuO|Y1PThizszN
CP66-MELNVq3SIdkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MofrTWM2OD1|Lke5NFk1KM7:TR?=
NCI-H69MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NHzHXVdKSzVyPUSuNFE6OzZizszN
A253MlrRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MXPJR|UxRTRwMEKxNFEh|ryP
NB14NYPQXoRjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NEHYV5ZKSzVyPUSuNVA1PzlizszN
NCI-H1694NIK3XYxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NWn5dok3UUN3ME20MlE{OTF{IN88US=>
NCI-H2196M4iwNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NEjQeJVKSzVyPUSuNVcyPjlizszN
TE-9MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MXnJR|UxRTRwMUe1PFIh|ryP
D-283MEDM2i3Zmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NYD2d4Q1UUN3ME20MlE5QDRizszN
OCI-AML2MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NY\IVotpUUN3ME20MlE6PDh7IN88US=>
D-263MGNFTtR|BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NHTsfXNKSzVyPUSuNlI6PjFizszN
MPP-89NWXYVINWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MoHnTWM2OD12LkK3N|A1KM7:TR?=
LAMA-84NFj0RXRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MUDJR|UxRTRwM{C0NlEh|ryP
LB373-MEL-DMofkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NHHuVI9KSzVyPUSuN|Y4QDlizszN
UACC-257MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?Mn7hTWM2OD12LkO5OVM1KM7:TR?=
MC-CARNE\HNVdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MkHITWM2OD12LkSzPVkh|ryP
COLO-320-HSRM3;ocmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NY\ObphiUUN3ME20MlQ1PDJ5IN88US=>
P30-OHKMYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NGj1VXhKSzVyPUSuOlY2QDFizszN
UACC-812MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NUHaXphZUUN3ME20MlY6OTZzIN88US=>
CTB-1M{i2TGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NXXEV|B4UUN3ME20MlcyPTV3IN88US=>
ALL-PONV7Gd29xT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NEGwcJNKSzVyPUSuPFQxPzdizszN
SK-MEL-2Ml3XS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MnL1TWM2OD12Lki2PVU2KM7:TR?=
TC-YIKNHraXIVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=M3rH[2lEPTB;ND65O|k1OiEQvF2=
NCI-H1882Mln2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MlLaTWM2OD13LkCyNFAyKM7:TR?=
MHH-CALL-2MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MkjhTWM2OD13LkC1NFQzKM7:TR?=
U-87-MGM{jSbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MVLJR|UxRTVwMEm0OlYh|ryP
NCI-H1092Mm[zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NGfKeWhKSzVyPUWuNlY2PTVizszN
TE-441-TNXvtN5dmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NH:wdYFKSzVyPUWuNlc5OiEQvF2=
SK-MEL-1NUCz[md6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=Ml\kTWM2OD13LkK5NFQ1KM7:TR?=
EW-22M{HnSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NHzUZVBKSzVyPUWuNlk1PjZizszN
MZ7-melMWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MlrKTWM2OD13LkSwOlkyKM7:TR?=
LP-1MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NYTTe3lxUUN3ME21MlQyOjlzIN88US=>
NCI-SNU-16M2DOWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7Mo[zTWM2OD13Lk[0NFc1KM7:TR?=
LU-65NFrndohIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=Mn25TWM2OD13Lke2N|c{KM7:TR?=
CW-2MnLxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?Mmn1TWM2OD13Lki1PVU6KM7:TR?=
WSU-NHLMoryS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MVzJR|UxRTVwOUWxO|Qh|ryP
IST-MES1MkP0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?Mkf6TWM2OD13Lkm1OFQ{KM7:TR?=
U-266MlnsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MYfJR|UxRTVwOUiyNFIh|ryP
TALL-1MnnyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NV\SZo9sUUN3ME22MlE1Pjh6IN88US=>
Calu-6MnrqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NHzsZmhKSzVyPU[uNVU{OTZizszN
MMAC-SFNVf2VG84T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=Ml23TWM2OD14LkG4OVU3KM7:TR?=
NCI-H82MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?Mkn1TWM2OD14LkKwOFg6KM7:TR?=
RS4-11MmXkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MYfJR|UxRTZwMkW4PVch|ryP
SNU-C2BNEL0eVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=M3u5eGlEPTB;Nj60NFk3QSEQvF2=
BOKUM{D0eWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MX\JR|UxRTZwNEe1PVch|ryP
C8166NFLJV4pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=M3y1bWlEPTB;Nj61OVkyOiEQvF2=
D-247MGNH72OZlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MmG0TWM2OD15LkC0N|Q4KM7:TR?=
EW-18NHX4RVFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NHu1WHlKSzVyPUeuNFczQTJizszN
KG-1MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MlH4TWM2OD15Lk[yO|M5KM7:TR?=
REHNF;DRpZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NWfNPJZGUUN3ME23MlY5OTB7IN88US=>
U-698-MNVjOb2tsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=Ml7LTWM2OD15Lki0N|E2KM7:TR?=
KP-N-RT-BM-1M1SzcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MYPJR|UxRTdwOUOwNlkh|ryP
MS-1MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MkfsTWM2OD15Lkm2NFQyKM7:TR?=
SNU-C1M3;DW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7M{i0NmlEPTB;Nz65PFE6OiEQvF2=
SK-MM-2MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NWTz[HFkUUN3ME24MlI3ODZ3IN88US=>
LAN-6M322TGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NIDXTZlKSzVyPUiuN|AxODFizszN
NEC8NX7IU2F[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NWr2VpNZUUN3ME24MlMxPjlzIN88US=>
NCI-H1770M3nqNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MmjLTWM2OD16LkO4NFAzKM7:TR?=
D-336MGMmSzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MmfsTWM2OD16LkSwNVE3KM7:TR?=
COLO-829NInWNHVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NVLoSIFiUUN3ME24MlQ5QDd7IN88US=>
LS-513M3zWemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NGTuN5dKSzVyPUiuOVk2QTlizszN
YTMX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NILhPGlKSzVyPUiuOlI1OjdizszN
EW-24MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MWPJR|UxRThwN{[1OEDPxE1?
IST-SL1M2ToT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7MUHJR|UxRThwOE[1OFMh|ryP
CA46M1\mcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NHXWcVNKSzVyPUiuPVUxQThizszN
NCI-H1838Mn;kS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NVfHXmFKUUN3ME24Mlk5PjB{IN88US=>
NCI-H719MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NUK2e5NUUUN3ME25MlI2Ojd7IN88US=>
HCE-TNXj0d2JTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NGrQV5ZKSzVyPUmuN|A5PTFizszN
A498M1nGfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MYTJR|UxRTlwM{[xNlQh|ryP
LB831-BLCNXPNUWNnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NXjtTIRqUUN3ME25Mlc3PTJzIN88US=>
SKM-1NYLqVZFmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NEnSO3dKSzVyPUmuPFU6PjNizszN
THP-1NF\JVYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NU\EU|BFUUN3ME25Mlk3QTF6IN88US=>
SHP-77MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NEfTfYZKSzVyPUGwMlQxPyEQvF2=
EW-3NGPCT3NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NELFblNKSzVyPUGwMlYzQDlizszN
KY821MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MorWTWM2OD1zMD63OlMh|ryP
NCI-SNU-1MofOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?Mlj0TWM2OD1zMT6wNlE4KM7:TR?=
HCC2218MnPtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?M3uweWlEPTB;MUGuN|k5PiEQvF2=
IM-9MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NXq3WVM1UUN3ME2xNU42OTB4IN88US=>
NCI-H889MoHwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MWXJR|UxRTFzLkWzNVMh|ryP
HDLM-2NVy2WJBjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NG\zcmJKSzVyPUGyMlQyPTlizszN
LB2518-MELMVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MkjwTWM2OD1zMj62PFE2KM7:TR?=
NCI-H23NXLLU3hnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MmHVTWM2OD1zMz6yOFI2KM7:TR?=
NB17Mom3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MnvoTWM2OD1zMz60OVc6KM7:TR?=
NCI-H322MMYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MmnRTWM2OD1zND60NFY5KM7:TR?=
SUP-T1MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M33CV2lEPTB;MUSuOFE{KM7:TR?=
ES3NEHoeJpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MkH6TWM2OD1zNT6wO|A{KM7:TR?=
ES5MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MlTSTWM2OD1zNT6wO|g4KM7:TR?=
NCI-H1650NVvQSlR[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MlLXTWM2OD1zNT60PVc6KM7:TR?=
NCI-H226NWDPeWtxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MVjJR|UxRTF3Lki3Olgh|ryP
COR-L88Mmf6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NFe4VIdKSzVyPUG2MlMyPCEQvF2=
SCC-15MlvPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NF72SY5KSzVyPUG2MlM5PjlizszN
GOTOMoHtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NX3OWnNTUUN3ME2xOk41Pzl|IN88US=>
SIMANV65OYU3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NWLkVVlzUUN3ME2xOk41QDB{IN88US=>
NCI-H1299NFLLZ4NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NHvRNYJKSzVyPUG3MlE2QTFizszN
NCI-H1581MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MYXJR|UxRTF5LkSyNVkh|ryP
MHH-NB-11M{fiV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7M3jhdWlEPTB;MUeuPVY5OyEQvF2=
MFM-223M{HzSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MmfJTWM2OD1zOD6wOVM5KM7:TR?=
ES7M{K5d2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7M4rSZ2lEPTB;MUiuOVQ{OSEQvF2=
JVM-3M2DKPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7NUT5TpY3UUN3ME2xPE44OTdizszN
RLMXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?NW\sV|FvUUN3ME2yNE4{QDhizszN
EC-GI-10MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?MYjJR|UxRTJzLkKwOFEh|ryP
LNCaP-Clone-FGCNVLUeYxtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=NU\IOodZUUN3ME2yNU43PzZ6IN88US=>
IMR-5NIjBVGJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NFjoT5NKSzVyPUKxMlg1QTRizszN
KP-N-YSMnTuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NUjCb4ZYUUN3ME2yNU45PzVizszN
Mo-TNGLv[nNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=MmnvTWM2OD1{Mj6yNVg2KM7:TR?=
NCI-H128NYjyXYZzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm=MYfJR|UxRTJ|LkW4OVMh|ryP
RH-1NEXDWIhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NVmyOmxPUUN3ME2yN{44QDZ4IN88US=>
NCI-H2171NEL6U3hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=M1XsO2lEPTB;MkSuNlQ5PSEQvF2=
RPMI-8866MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?M3TLOmlEPTB;Mk[uO|QzKM7:TR?=
SK-N-FIM3jOeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7MnXtTWM2OD1{Nz6zPFEyKM7:TR?=
LOXIMVIMoTYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?MnnMTWM2OD1{Nz64NFUyKM7:TR?=
P31-FUJMmjnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?NYDlTY1yUUN3ME2zNU42Ozd2IN88US=>
KMS-12-PENGHnfHdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=NYj0[VY2UUN3ME20PU42OzB{IN88US=>

... Click to View More Cell Line Experimental Data

In vivo Treatment with Rapamycin in vivo specifically blocks targets known to be downstream of mTOR such as the phosphorylation and activation of p70S6K and the release of inhibition of eIF4E by PHAS-1/4E-BP1, leading to complete blockage of the hypertrophic increases in plantaris muscle weight and fibre size. [4] Short-term Rapamycin treatment, even at the lowest dose of 0.16 mg/kg, produces profound inhibition of p70S6K activity, which correlates with increased tumor cell death and necrosis of the Eker renal tumors. [5] Rapamycin inhibits metastatic tumor growth and angiogenesis in CT-26 xenograft models by reducing the production of VEGF and blockage of VEGF-induced endothelial cell signaling. [6] Rapamycin treatment at 4 mg/kg/day significantly reduces tumor growth of C6 xenografts, and tumor vascular permeability. [7]
Features

Protocol(Only for Reference)

Kinase Assay:

[1]

Immunoblotting for the mTOR kinase assay HEK293 cells are plated at 2-2.5×105 cells/well of a 12-well plate and serum-starved for 24 hours in DMEM. Cells are treated with increasing concentrations of Rapamycin (0.05-50 nM) for 15 minutes at 37 °C. Serum is added to a final concentration of 20% for 30 minutes at 37 °C. Cells are lysed, and cell lysates are separated by SDS-PAGE. Resolved proteins are transferred to a polyvinylidene difluoride membrane and immunoblotted with a phosphospecific primary antibody against Thr-389 of p70 S6 kinase. Data are analyzed using ImageQuant and KaleidaGr

Cell Assay:

[3]

Cell lines U87-MG, T98G, and U373-MG
Concentrations Dissolved in DMSO, final concentrations ~25 μM
Incubation Time 72 hours
Method

Cells are exposed to various concentrations of Rapamycin for 72 hours. For the assessment of cell viability, cells are collected by trypsinization, stained with trypan blue, and the viable cells in each well are counted. For the determination of cell cycle, cells are trypsinized, fixed with 70% ethanol, and stained with propidium iodide using a flow cytometry reagent set. Samples are analyzed for DNA content using a FACScan flow cytometer and CellQuest software. For apoptosis detection, cells are stained with the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) technique using an ApopTag apoptosis detection kit. To detect the development of acidic vesicular organelles (AVO), cells are stained with acridine orange (1 μg/mL) for 15 minutes, and examined under a fluorescence microscope. To quantify the development of AVOs, cells are stained with acridine orange (1 μg/mL) for 15 minutes, removed from the plate with trypsin-EDTA, and analyzed using the FACScan flow cytometer and CellQuest software. To analyze the autophagic process, cells are incubated for 10 minutes with 0.05 mM monodansylcadaverine at 37 °C and are then observed under a fluorescence microscope.

Animal Study:

[7]

Animal Models Athymic Nu/Nu mice inoculated subcutaneously with VEGF-A-expressing C6 rat glioma cells
Formulation Dissolved in solvent solution (0.2% carboxymethylcellulose and 0.25% Tween-80 in sterile H2O)
Dosages ~4 mg/kg/day
Administration Injection i.p.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

SpeciesMouseRatRabbitGuinea pigHamsterDog
Weight (kg)0.020.151.80.40.0810
Body Surface Area (m2)0.0070.0250.150.050.020.5
Km factor36128520
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Rat dose (mg/kg) = mouse dose (22.4 mg/kg) ×  mouse Km(3)  = 11.2 mg/kg
rat Km(6)
1

References

[1] Edwards SR, et al. J Biol Chem, 2007, 282(18), 13395-13401.

[2] Barbet NC, et al. Mol Biol Cell, 1996, 7(1), 25-42.

view more

Clinical Trial Information( data from http://clinicaltrials.gov, updated on 2015-08-29)

NCT Number Recruitment Conditions Sponsor
/Collaborators
Start Date Phases
NCT02240407 Not yet recruiting Pompe Disease University of Florida January 2016 Phase 1
NCT02528877 Not yet recruiting Acute Myeloid Leukemia in Remission|Primary Myelofibrosis|Primary Myelofibrosis, Prefibrotic Stage|Secondary Acute Myeloid Leukemia|Secondary Myelo  ...more Acute Myeloid Leukemia in Remission|Primary Myelofibrosis|Primary Myelofibrosis, Prefibrotic Stage|Secondary Acute Myeloid Leukemia|Secondary Myelofibrosis City of Hope Medical Center|National Cancer Institute (NCI) December 2015 Phase 1
NCT02456857 Not yet recruiting Breast Cancer M.D. Anderson Cancer Center November 2015 Phase 2
NCT02509468 Not yet recruiting Vascular Malformation University Hospital, Tours September 2015 Phase 2
NCT02524639 Not yet recruiting Hyperinsulinism Childrens Hospital of Philadelphia August 2015 Phase 1|Phase 2

view more

Chemical Information

Download Rapamycin (Sirolimus) SDF
Molecular Weight (MW) 914.18
Formula

C51H79NO13

CAS No. 53123-88-9
Storage 3 years -20℃powder
6 months-80℃in solvent
Synonyms AY 22989,NSC-2260804
Solubility (25°C) * In vitro DMSO 20 mg/mL (21.87 mM)
Water <1 mg/mL (<1 mM)
Ethanol <1 mg/mL (<1 mM)
In vivo 0.5% CMC/0.25% Tween 80 30 mg/mL
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Name (3S,6R,7E,9R,10R,12R,14S,15E,17E,19E,21S,23S,26R,27R,34aS)-9,10,12,13,14,21,22,23,24,25,26,27,32,33,34,34a-Hexadecahydro-9,27-dihydroxy-3-[(1R)-2-[(1S,3R,4R)-4-hydroxy-3-methoxycyclohexyl]-1-methylethyl]-10,21-dimethoxy-6,8,12,14,20,26-hexamethyl-23,27-epoxy-3H-pyrido[2,1-c][1,4]oxaazacyclohentriacontine-1,5,11,28,29(4H,6H,31H)-pentone

Customer Product Validation (12)


Click to enlarge
Rating
Source Nat Genet 2014 46(4), 364-70. Rapamycin (Sirolimus) purchased from Selleck
Method Western blot
Cell Lines Persister cells
Concentrations 10 nM
Incubation Time 3 days
Results While added with a specific mTOR inhibitor, rapamycin(Rapa), it inhibitied endogenous mTOR activity, showed that markedly reduced MYC protein levels in persister cells but not in naive T-ALL cells.

Click to enlarge
Rating
Source Cancer Cell 2011 19(6), 792-804. Rapamycin (Sirolimus) purchased from Selleck
Method Cell viability Analysis
Cell Lines Ar+murine (CaP8) and human (LNCaP) prostate cancer cells, Pb-Cre+;PtenL/L mice, Pb-Cre+;PtenL/L;ArL/Y mice
Concentrations 1 nM, 4 mg/kg
Incubation Time 0-4 weeks
Results These data suggest that CaPs with AR loss have greater reliance upon the PI3K/AKT/mTOR-signaling pathways and that combined AR/androgen blockage in conjunction with PI3K/AKT/mTOR inhibition (by Rapamycin) is more effective for CaPs initiated by PTEN loss or PI3K/AKT activation.

Click to enlarge
Rating
Source Cell Res 2012 22(6), 1003-21. Rapamycin (Sirolimus) purchased from Selleck
Method EdU labeling
Cell Lines MCF-7 cells
Concentrations 150 nM
Incubation Time 48 h
Results The punctate structures from SRC-3 or MIF knockdown cells are very similar to that found in cells treated with rapamycin, a known inducer of autophagy.

Click to enlarge
Rating
Source Autophagy 2015 11(4), 617-28. Rapamycin (Sirolimus) purchased from Selleck
Method Cell imaging
Cell Lines H4-LC3-GFP cells
Concentrations 200 nM
Incubation Time 24 h
Results Consistent with inhibition of autophagy, the levels of LC3-II were lower and the levels of SQSTM1/p62 were higher in this USP18 knockdown cell line compared to that of control under basal conditions as well as the induced condition after the treatment with rapamycin.

Click to enlarge
Rating
Source Cell Rep 2015 10.1016/j.celrep.2015.01.014. Rapamycin (Sirolimus) purchased from Selleck
Method Western blotting
Cell Lines HEK293 cells
Concentrations 250 nM
Incubation Time 24 h
Results Rapamycin virtually abolishes stimulation by Rheb of phospho-S6 kinase and phospho-4EBP1 but has negligible effects on phospho-eIF2a (A). In contrast to phosphorylation of eIF2a, which is not mediated by mTORC1, phosphorylation of other elongation factors does involve mTORC1. Thus, rapamycin prevents phosphorylation of eIF4G and eIF4B, targets of S6K, but not eIF2a (B). By contrast, rapamycin, as expected, abolishes phospho-S6 kinase but fails to influence phospho-eIF2a, phospho-Erk or phospho-Akt (C).

Click to enlarge
Rating
Source Biochem Pharmacol 2015 95(3), 156-69. Rapamycin (Sirolimus) purchased from Selleck
Method Immunofluorescence
Cell Lines RAW264.7 cells
Concentrations 10 uM
Incubation Time 16 h
Results It has been reported that autophagy can directly regulate inflammatory responses. To confirm this phenomenon, we pre-treated RAW264.7 cells with 3-MA (an autophagy inhibitor by blocking the class III PI3Ks) or rapamycin (an autophagy inducer by targeting the negative regulators against autophagy as mammalian target of rapamycin (mTOR)), followed by addition of LPS. LPS-induced punctate LC3 was markedly reduced and enhanced in the presence of 3-MA and rapamycin, respectively.

Click to enlarge
Rating
Source Biochem Pharmacol 2011 82, 216-226. Rapamycin (Sirolimus) purchased from Selleck
Method Western blot
Cell Lines H1299 cells
Concentrations 2 μM
Incubation Time 48 h
Results RAD001 and other mTOR inhibitors decreased the levels of survivin protein, as assessed by Western blot analysis, without affecting the levels of other IAP members. In addition, combined treatment with sorafenib and mTOR inhibitor Rapamycin and RAD001 decreased survivin expression to a greater extent than treatment with either alone.

Click to enlarge
Rating
Source J Lipid Res 2011 52, 1617-1625. Rapamycin (Sirolimus) purchased from Selleck
Method Histological analysis, immunohistochemistry, Liver triglyceride content analysis, real-time PCR
Cell Lines Male SD rats
Concentrations
Incubation Time 7 d
Results To determine the effect of rapamycin, an mTOR inhibitor, in the OA-induced fatty liver, rats were fed 1% OA and administered rapamycin for seven days. OA-induced lipid accumulation was completely inhibited by the treatment with rapamycin (Fig. A). HE and Oil Red O staining of liver sections clearly confi rmed the results (Fig. B). Immunohistochemistry analyses showed signifi cantly repressed SREBP-1 in the rapamycin-cotreatment liver ( Fig. B ). All the downstream effectors of SREBP-1, such as Lxr-α, Acc, Scd-1 and Fas, were consistently suppressed by rapamycin ( Fig. C ).

Click to enlarge
Rating
Source Biochim Biophys Acta 2013 1833(3), 652-62. Rapamycin (Sirolimus) purchased from Selleck
Method Measurement of cytosolic free Ca2+concentration ([Ca2+]c )
Cell Lines Human platelets
Concentrations 500 nM
Incubation Time 30 min
Results Rapamycin administration significantly reduced TG-evoked Ca2+ -entry by 19. 5 ?9.4 %.

Click to enlarge
Rating
Source Tuberc Respir Dis 2013 75(1), 9-17. Rapamycin (Sirolimus) purchased from Selleck
Method Microscope imaging
Cell Lines NCI-H1299 cells
Concentrations 10 uM
Incubation Time 48 h
Results Growth inhibition induced by rapamycin or erlotinib is enhanced by combination treatment with monensin in NCI-H1299 cells.

Click to enlarge
Rating
Source 2013 Dr. Zhang of Tianjin Medical University. Rapamycin (Sirolimus) purchased from Selleck
Method Western Blot
Cell Lines HeLa cells
Concentrations 0/2/20/200 nM
Incubation Time 24 h
Results

Click to enlarge
Rating
Source 2011 Dr.Ulrich Bommer of University of Wollongong. Rapamycin (Sirolimus) purchased from Selleck
Method Western blot
Cell Lines
Concentrations
Incubation Time 0-6 h
Results Rapamycin inhibits growth-dependent TCTP induction.

Product Use Citation (54)

Tech Support & FAQs

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

* Indicates a Required Field

Related mTOR Products

  • Zotarolimus(ABT-578)

    Zotarolimus (ABT-578) is an analogue of rapamycin, and inhibits FKBP-12 binding with IC50 of 2.8 nM.

    Features:Zotarolimus has a shorter in vivo half-life and is also demonstrated in rats to have less potent systemic immunosuppression than rapamycin.

  • MHY1485

    MHY1485 is a potent, and cell-permeable mTOR activator, and also potently inhibits autophagy.

  • LY2090314

    LY2090314 is a potent GSK-3 inhibitor for GSK-3α/β with IC50 of 1.5 nM/0.9 nM; may improve the efficacy of platinum-based chemotherapy regimens. Phase 2.

  • Everolimus (RAD001)

    Everolimus (RAD001) is an mTOR inhibitor of FKBP12 with IC50 of 1.6-2.4 nM in a cell-free assay.

  • AZD8055

    AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. Phase 1.

    Features:First drug to inhibit both types of mTOR protein.

  • INK 128 (MLN0128)

    INK 128 (MLN0128) is a potent and selective mTOR inhibitor with IC50 of 1 nM in cell-free assays; >200-fold less potent to class I PI3K isoforms, superior in blocking mTORC1/2 and sensitive to pro-invasion genes (vs Rapamycin). Phase 1.

  • Temsirolimus (CCI-779, NSC 683864)

    Temsirolimus (CCI-779, NSC 683864) is a specific mTOR inhibitor with IC50 of 1.76 μM in a cell-free assay.

  • Torin 1

    Torin 1 is a potent inhibitor of mTORC1/2 with IC50 of 2 nM/10 nM in cell-free assays; exhibits 1000-fold selectivity for mTOR than PI3K.

  • Tacrolimus (FK506)

    Tacrolimus (FK506) is a 23-membered macrolide lactone, it reduces peptidyl-prolyl isomerase activity in T cells by binding to the immunophilin FKBP12 (FK506 binding protein) creating a new complex.

  • Torkinib (PP242)

    Torkinib (PP242) is a selective mTOR inhibitor with IC50 of 8 nM in cell-free assays; targets both mTOR complexes with >10- and 100-fold selectivity for mTOR than PI3Kδ or PI3Kα/β/γ, respectively.

    Features:One of the first selective inhibitors that targets ATP domain of mTOR.

Recently Viewed Items

Tags: buy Rapamycin (Sirolimus) | Rapamycin (Sirolimus) ic50 | Rapamycin (Sirolimus) price | Rapamycin (Sirolimus) cost | Rapamycin (Sirolimus) solubility dmso | Rapamycin (Sirolimus) purchase | Rapamycin (Sirolimus) manufacturer | Rapamycin (Sirolimus) research buy | Rapamycin (Sirolimus) order | Rapamycin (Sirolimus) mouse | Rapamycin (Sirolimus) chemical structure | Rapamycin (Sirolimus) mw | Rapamycin (Sirolimus) molecular weight | Rapamycin (Sirolimus) datasheet | Rapamycin (Sirolimus) supplier | Rapamycin (Sirolimus) in vitro | Rapamycin (Sirolimus) cell line | Rapamycin (Sirolimus) concentration | Rapamycin (Sirolimus) nmr
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description
Contact Us