MG-132

Catalog No.S2619

MG-132 Chemical Structure

Molecular Weight(MW): 475.62

MG-132 is an inhibitor of proteasome with IC50 of 100 nM in a cell-free assay, and also inhibits calpain with IC50 of 1.2 μM.

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In DMSO USD 100 In stock
USD 50 In stock
USD 170 In stock
USD 470 In stock

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Cited by 47 Publications

10 Customer Reviews

  • MDA-MB-231 cells were treated with 10 uM MG132 and incubated under normoxia or hypoxia for 4 h. Endogenous interaction between LATS2 and SIAH2 was analysed by immunoprecipitation.

    Nat Cell Biol 2015 17(1), 95-103. MG-132 purchased from Selleck.

    HeLa cells were transfected with control mimic, RMND5A siRNA or miR-138 mimic. After transfection for 36 h, cells were treated with proteasome inhibitor MG132 (20 uM) or Dimethyl sulfoxide (DMSO) for 8 h. Endogenous Exportin-5 protein and RMND5A protein expression was measured by western blotting.

    Nucleic Acids Res 2014 42(1), 458-74. MG-132 purchased from Selleck.

  • HeLa cells were transfected with negative control mimic, miR-138 mimic or RMND5A siRNA. After transfection for 36 h, cells were treated with MG132 (20 uM) or DMSO for 8 h. RMND5A and Exportin-5 protein expression were analyzed by immunocytochemistry. Nuclei were counterstained with DAPI.

    Nucleic Acids Res 2014 42(1), 458-74. MG-132 purchased from Selleck.

     

    TFEB induced autophagy dependent ATZ clearance in vitro. MEF transiently co-transfected with either ATZ plus GFP expressing plasmids or ATZ plus TFEB expressing plasmids were incubated with proteasome inhibitor MG132 or with DMSO for 6 h. Upon MG132 treatment, steady state levels of 52 kDa ATZ band increased in both cells transfected with ATZ plus GFP and with ATZ plus TFEB expressing plasmids. Quantification of band intensities showed a 48% reduction of ATZ in cells transfected with ATZ plus TFEB compared to cells transfected with ATZ plus GFP, in the absence of the proteasome inhibitor. In MG132 treated cells, a 61% decrease of ATZ was detected in cells transfected with ATZ plus TFEB compared to cells transfected with ATZ plus GFP.

    EMBO Mol Med 2013 5, 397-412. MG-132 purchased from Selleck.

  • JAK1 expression was analyzed in A375 cells expressing scrambled shRNA or shRNF125 (#2) and treated with MG132 (5 uM, 10 hr), and in Lu1205 cells expressing WT RNF125 and treated with MG132.

    Cell Rep 2015 11(9), 1458-73. MG-132 purchased from Selleck.

    LRB1 and LRB2 regulate phyB protein levels. PhyB protein levels in crude extracts from the wild type (WT) and the indicated mutant backgrounds were assayed by immunoblot analysis with anti-phyB monoclonal antibodies. Anti-PBA1 and anti-histone H3 antibodies were used to confirm equal protein loading. Rc-induced loss of phyB in the wild type is inhibited by 100 uM MG132.

    Plant Physiol 2012 60(1), 118-34. MG-132 purchased from Selleck.

  • Representative images for γ-H2AX-stained cells treated with 100 uM FA for 3 h without or with MG132. Fluorescence was measured in at least 50 nuclei in each of three experiments.

    Toxicol Appl Pharmacol 2015 286(2), 135-41. MG-132 purchased from Selleck.

    GSK-3β phosphorylation at Ser9 is enhanced in NOK-overexpressed stable cell lines. The proteasome inhibitor, MG132, was added in medium of HeLa-HA and HeLa-NOK-HA cells for 12 h and then the p-GSK-3b(Ser9) was assayed by western blotting.

    FEBS Lett 2012 586, 3787–3792. MG-132 purchased from Selleck.

  • Nek2A impedes SuFu ubiquitination. HEK293T cells transfected with Nek2A-Myc and Flag-SuFu for 48 h were treated with MG-132 to enrich ubiquitinated proteins, and subsequently subjected to co-immunoprecipitation assay. Precipitated proteins were probed using anti-Ub antibody.

    Int J Oncol, 2017, 50(2):373-380. MG-132 purchased from Selleck.

    Truncated mouse Period2 (Per2) protein was expressed in NIH3T3 cells, treated by cycloheximide with or without MG132 for 1-4 hours.Per2 was reported to be degraded by the proteasome proteolysis. By adding MG132 (25 μM), a proteasome inhibitor, the degradation of Per2 was dramatically decreased within 4 hours treatment (shown by western blot).

    2012 Dr. Edison DUKE-NUS Graduate Medical School Singapore.. MG-132 purchased from Selleck.

Purity & Quality Control

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Notes:

2. For more details, such as half maximal inhibitory concentrations (IC50s) and working concentrations of each inhibitor, please click on the link of the inhibitor of interest.
3. "+" indicates inhibitory effect. Increased inhibition is marked by a higher "+" designation.
4. Orange "√" refers to compounds which do inhibitory effects on the related isoform, but without specific value.

Biological Activity

Description MG-132 is an inhibitor of proteasome with IC50 of 100 nM in a cell-free assay, and also inhibits calpain with IC50 of 1.2 μM.
Targets
Proteasome [1]
(Cell-free assay)
100 nM
In vitro

MG-132 displays >1000 times more activity than ZLLal in inhibiting the ZLLL-MCA-degrading activity of 20S proteasome with IC50 of 100 nM versus 110 μM. MG-132 also inhibits calpain with IC50 of 1.2 μM. MG-132 induces neurite outgrowth in PC12 cells at an optimal concentration of 20 nM, displaying 500 times more potency than ZLLal. [1] MG-132 (10 μM) potently inhibits TNF-α-induced NF-κB activation, interleukin-8 (IL-8) gene transcription, and IL-8 protein release in A549 cells by inhibition of proteasome-mediated IκBα degradation. [2] MG-132 treatment potently induces p53-dependent apoptosis in KIM-2 cells by 26S proteasome inhibition. [3] Unlike BzLLLCOCHO or PS-341, MG-132 treatment results in weak inhibition of the chymotrypsinlike (CT-L) and peptidylglutamyl peptide hydrolysing (PGPH) activities of the 26S proteasome, whereas multiple myeloma cells (U266 and OPM-2) are more sensitive to induction of apoptosis by MG-132 than BzLLLCOCHO. [4] MG-132 (1 μM) sensitizes TRAIL-resistant prostate cancer cells by activating the AP-1 family members c-Fos and c-Jun, which, in turn, repress the antiapoptotic molecule c-FLIP(L). [5] MG-132 significantly enhances the ability of inositol hexakisphosphate (IP6) to reduce cellular metabolic activity in both PC3 and DU145 androgen-independent prostate cancer (AIPCa) cell lines. [6]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LPS-stimulated RAW264.7 cells NVzldm5rTnWwY4Tpc44hSXO|YYm= MoTDNlUh|ryP M3X0XWROW09? M{jXNmlvcGmkaYTzJI5qfHKrYzDvfIll\SCycn;keYN1cW:wIIfpeIghUUN3MDDv[kAxNjFizszN MmXTNlQ3QTd2OU[=
HL-60 M{i5OGN6fG:2b4jpZ{BCe3OjeR?= MV:0NEDPxE1? MnTyOFghcA>? MYDEUXNQ NUTMV|FoUUN3MEyxNEDPxE1? M4L2dFI1Pjl5NEm2
SMMC-7721 M3HoVmN6fG:2b4jpZ{BCe3OjeR?= NHnrV3E1OCEQvF2= NH\UUHA1QCCq NVHvdZdUTE2VTx?= M1yxUmlEPTB;Nz6xJO69VQ>? NF\RWIYzPDZ7N{S5Oi=>
A-549 M{PjO2N6fG:2b4jpZ{BCe3OjeR?= NUjES|I2PDBizszN NWLpR4l[PDhiaB?= MWXEUXNQ MoWzTWM2ODxzMDFOwG0> MYmyOFY6PzR7Nh?=
MCF-7 MWHDfZRwfG:6aXOgRZN{[Xl? MVe0NEDPxE1? MVy0PEBp M3m4S2ROW09? MkDSTWM2OD15LkOg{txO NYnxWZY4OjR4OUe0PVY>
SW-480 NHvsOHpEgXSxdH;4bYMhSXO|YYm= NV72SmFzPDBizszN NITnRpo1QCCq MUXEUXNQ NH3mUo9KSzVyPUSg{txO M4[1PVI1Pjl5NEm2
NCI-H929 M162UWN6fG:2b4jpZ{BCe3OjeR?= MkDQNUDPxE1? M17WUFczKGh? NVzSd|ZSTE2VTx?= MmP3TWM2OD1yLkG3JO69VQ>? MnnqNlQ3OjVyOEi=
293T MoLLR5l1d3SxeHnjJGF{e2G7 NESySIoyOCEQvF2= NVfpPVZtPzJiaB?= NXPCVHp[TE2VTx?= NGTEZYdKSzVyPEKg{txO MWGyOFYzPTB6OB?=
293T MUXGeY5kfGmxbjDBd5NigQ>? NVPae2pbOTBizszN NUK3dplkOjRiaB?= Mlm3SG1UVw>? NYL0U2psVW:mZYLheIVtgSCrbnT1Z4V{KGinYYStd4hw[2tiIB?= NFTJemszPDZ{NUC4PC=>
HeLa MUjLbY5ie2ViQYPzZZk> M2iy[FExKM7:TR?= NVvlcGdNOSCq NInpd5dFVVOR MkD5TY5lfWOnczDQRXJRKGOuZXH2ZYdmKGK7IHnubIljcXSrbnegdJJwe2Wjc3;t[UBi[3Srdnn0fS=> MkTKNlQ{OjF6M{O=
MDA-MB-231 NYKwfmxKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGnXTHIyKM7:TR?= NH2zR5U4OiCq MkfBSG1UVw>? M3nz[WlEPTB;MD6xPEDPxE1? NGO2PJczPDF3M{KwOi=>
MCF-7 NWnHc3NyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2\FcFEh|ryP MonJO|IhcA>? M3HIVWROW09? NFn5[nNKSzVyPUCuNVMh|ryP MXOyOFE2OzJyNh?=
MCF10A NILLdW9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWKxJO69VQ>? MVS3NkBp NX\KXXZ5TE2VTx?= MVzJR|UxRTBwMkmg{txO NIfle4UzPDF3M{KwOi=>
HEK-293 MkezT4lv[XOnIFHzd4F6 M{HPXlIh[W6mIEKwJO69VQ>? NFeyb4kzKGh? NHrZcJBFVVOR NWDIV2h6UW6qaXLpeJMhS2iWLVygZYN1cX[rdImge4l1cCCLQ{WwJI9nKDlibl2g NFPzblQzOzV2MEe5NC=>
Calu6 NIfUcFVHfW6ldHnvckBCe3OjeR?= Mon0NVAh|ryP MoPFNVghcA>? NXzGS|lsTE2VTx?= NYHGSZByW2mpbnnmbYNidnSueTDhZ4N2dXWuYYTld{BnemG2YYjpckBxemWldYLzc5I> MWCyN|UxPjR6Nh?=
IFN-gamma-induced RAW264.7 M1Kwc2Z2dmO2aX;uJGF{e2G7 NIiwR4tFVVOR NVPNepRjUW6qaXLpeJMhdmm2cnnjJI95cWSnIIDyc4R2[3Srb36ge4l1cCCLQ{WwJI9nKDBwMEe3JO69VQ>? MkXwNlIzPzd{N{e=
IPC227F MlTrR5l1d3SxeHnjJGF{e2G7 NYjN[nF6OSEQvF2= MkXaOFghcA>? M{HjS2ROW09? NUi2bHdyUUN3ME2wMlA4PyEQvF2= MlHyNlE6OjR3Mki=
Hepa-1c1c7 MXHGeY5kfGmxbjDBd5NigQ>? NVnOeGo1OjVizszN M{fsRlYhcA>? NYHPNJN7TE2VTx?= MkHvTY5kemWjc3XzJG5z\jJicILveIVqdiCuZY\lcC=> NF\ENJgzODN7MkW0OC=>
COS-7 M{\HXmN6fG:2b4jpZ{BCe3OjeR?= MkjONVAh|ryP NYrsUnVpTE2VTx?= MWHJR|UxRDFyIN88US=> M1XKd|E5ODh6MEm3
HuH-7 M{PYfWN6fG:2b4jpZ{BCe3OjeR?= M4ewXFExKM7:TR?= NXLXWml1TE2VTx?= NFHRWYhKSzVyPEGwJO69VQ>? NFHSeYkyQDB6OEC5Oy=>
OCI-Ly3 NUXDdGc5TnWwY4Tpc44hSXO|YYm= MYexNEDPxE4EoB?= M1;nelQhcA>? MXHEUXNQ M4\Ed2lv\HWlZYOgbJVu[W5iSXvhdJBiSmGucHjhJJN1[WKrbHn6ZZRqd25id3n0bEBGSzVyIH;mJFEh|ryP NUn4Unk4OThyMkSxNVM>
A2780 cDDP MWDBdI9xfG:|aYOgRZN{[Xl? NWrhUG5bOjRiaB?= MVHEUXNQ MnPxTY5lfWOnczDhdI9xfG:|aYOgZpkhcW6qaXLpeIlv\yCSVFXOJIRm\3KjZHH0bY9v MVyxO|Y5PDBzOB?=
LPS-stimulated RAW264.7 cells NVvhcGFVTnWwY4Tpc44hSXO|YYm= MVnEUXNQ M3jtRWlvcGmkaYTzJG5HNWujcIDhRkBFVkFiYnnu[Ilv\w>? M37JVFE4PDB5Mke3
PC12 MUjGeY5kfGmxbjDBd5NigQ>? MUGxNFAh|ryPwrC= M13XUFI1KGh? M{L5TGROW09? MmfOTY5pcWKrdIOgOk1QUESDLTDhcoQhUDKRMj3pcoR2[2WmIHP5eI91d3irY3n0fS=> NX;PTJBoOTdzNUi0OVQ>
PC3 NWK0UVd[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHvlSYIzOCEQvF2= M2LLdFQ5KGh? MU\EUXNQ MmOzTWM2OD1yLk[g{txO NVTMOoJyOTZ4OE[1N|c>
LP-1 MmPBRZBweHSxc3nzJGF{e2G7 MorYN|AxKG6P NX6yXFlXOjRiaB?= MXnEUXNQ NULmW4lVUW6mdXPld{BieG:ydH;zbZMh[nliaX7jdoVie2mwZzDjcIVifmWmIGDBVnAhdGW4ZXygZY5lKHKnZIXjbY5oKE2lbD2xJJBzd3SnaX6gcIV3\Wx? NWPWU4JrOTV7NUi1PFk>
ES6 M4m3R2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX;4SlRqUUN3ME2wMlAyOzB4IN88US=> NF6ycoVUSU6JRWK=
A101D MornS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYfJR|UxRTBwMEOyO|kh|ryP NYHtO2c{W0GQR1XS
OCUB-M M1PQdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NILtdpBKSzVyPUCuNFM4OzdizszN MoqxV2FPT0WU
LB2518-MEL NWrO[lNET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnLETWM2OD1yLkCzO|Yh|ryP NWPYUVduW0GQR1XS
SH-4 MmDlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYPse4llUUN3ME2wMlA1OzFizszN NX;zbVV{W0GQR1XS
KNS-42 M2iwRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4\NXGlEPTB;MD6wOFQ1OSEQvF2= MnjSV2FPT0WU
DSH1 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYLJR|UxRTBwMEWyPFkh|ryP MmqwV2FPT0WU
NTERA-S-cl-D1 NGfqSYxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEnPSFhKSzVyPUCuNFU4PzJizszN MnroV2FPT0WU
D-542MG M37kUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHvTVG5KSzVyPUCuNFU6PTdizszN MVfTRW5ITVJ?
KS-1 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYnJR|UxRTBwME[0OVgh|ryP MkC0V2FPT0WU
BL-41 NGD5dWNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mm\oTWM2OD1yLkC2PVQ4KM7:TR?= M1TGZnNCVkeHUh?=
LXF-289 M4\4ZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYTHRZU5UUN3ME2wMlA4ODZ4IN88US=> MmHiV2FPT0WU
D-247MG NHnXcohIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmfVTWM2OD1yLkC3NlA6KM7:TR?= M2XSdHNCVkeHUh?=
MMAC-SF MnXkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlHlTWM2OD1yLkC3NlY5KM7:TR?= NYDvZ4s3W0GQR1XS
CP66-MEL MoLCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MU\JR|UxRTBwMEe1JO69VQ>? M3nNR3NCVkeHUh?=
LB771-HNC MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHjxb|RKSzVyPUCuNFgxOzZizszN NH;yfXJUSU6JRWK=
no-10 NWDuOnM2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVfRXoFEUUN3ME2wMlA5QTB7IN88US=> NX\F[VVNW0GQR1XS
A388 NVXnOow6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1vkUGlEPTB;MD6wPVA3PSEQvF2= NFj1N5NUSU6JRWK=
OPM-2 NVP3VINGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYjJR|UxRTBwMUC0O|Qh|ryP MkfrV2FPT0WU
OVCAR-4 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NE[2Z4pKSzVyPUCuNVA6OzhizszN MlTGV2FPT0WU
HOP-62 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{H6VWlEPTB;MD6xNFk1QSEQvF2= NWP3NFBkW0GQR1XS
ML-2 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYnJR|UxRTBwMUG4NFYh|ryP MYXTRW5ITVJ?
UACC-257 NITkbWdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NU\YeIh1UUN3ME2wMlEyQTJ5IN88US=> MUTTRW5ITVJ?
NEC8 NUHZclJNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYP2bm9rUUN3ME2wMlEyQTl3IN88US=> MVrTRW5ITVJ?
ONS-76 NWTaclJET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4jrXGlEPTB;MD6xNlk{PSEQvF2= MmTaV2FPT0WU
KE-37 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MU\JR|UxRTBwMUOyO|gh|ryP M3fxZ3NCVkeHUh?=
HT-144 NYLhZpN{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFTqdY9KSzVyPUCuNVM5QTVizszN NYL5ZWhkW0GQR1XS
LB2241-RCC MnjLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1SyXWlEPTB;MD6xOFI1PyEQvF2= MoLaV2FPT0WU
TE-5 NHHFPYtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlW2TWM2OD1yLkG0Nlc5KM7:TR?= NHSyNXFUSU6JRWK=
KINGS-1 Ml;aS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEnCVIJKSzVyPUCuNVQ4QSEQvF2= M4XPZnNCVkeHUh?=
NCI-H69 NU\xOYZWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnfZTWM2OD1yLkG1NVQyKM7:TR?= NUO2ZWZCW0GQR1XS
CAS-1 MnLaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFXBZ3JKSzVyPUCuNVU1QDJizszN MoDzV2FPT0WU
D-263MG NWrB[WtvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3TqSmlEPTB;MD6xOlAxPiEQvF2= MXvTRW5ITVJ?
A253 NEnvZ|ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXrwRpNEUUN3ME2wMlE3OTJ6IN88US=> M17CVnNCVkeHUh?=
PF-382 M2DkeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml;WTWM2OD1yLkG2O|A3KM7:TR?= M4TOS3NCVkeHUh?=
CESS M1HwPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWK2d4FtUUN3ME2wMlE4ODZizszN NVnqcWRCW0GQR1XS
MZ2-MEL MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVToellHUUN3ME2wMlE4PTV7IN88US=> MVPTRW5ITVJ?
HEL NWLvW2M6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYHJR|UxRTBwMUi1NVch|ryP M4XoXXNCVkeHUh?=
D-392MG MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MULJR|UxRTBwMUmxNVUh|ryP NEjicJlUSU6JRWK=
SK-LMS-1 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml[1TWM2OD1yLkG5N|A{KM7:TR?= NE\CU2RUSU6JRWK=
GI-ME-N MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVfGSI9oUUN3ME2wMlE6OzB4IN88US=> NFHVT|RUSU6JRWK=
LB831-BLC NUfYcnBIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnT0TWM2OD1yLkG5N|Qh|ryP NUfRdpU4W0GQR1XS
DU-4475 NXPKSVRIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVPJR|UxRTBwMUm2OVgh|ryP M1PvPHNCVkeHUh?=
IST-SL1 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVHJR|UxRTBwMkCwPVQh|ryP MY\TRW5ITVJ?
GAK M{nSOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUHOTJRTUUN3ME2wMlIxPTN2IN88US=> NFjPcHhUSU6JRWK=
EW-1 NVzaXHFjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4\NcmlEPTB;MD6yNVA1PyEQvF2= MkTyV2FPT0WU
LAMA-84 M{fySmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWTJR|UxRTBwMkG4OVEh|ryP NWHRXnRXW0GQR1XS
SK-UT-1 NWfrb29tT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYHJR|UxRTBwMkKwOFkh|ryP M3jueXNCVkeHUh?=
VA-ES-BJ MmfvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWDVSmV3UUN3ME2wMlIzOjV5IN88US=> MWfTRW5ITVJ?
ACN NHjB[lRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3XGVGlEPTB;MD6yNlY{QCEQvF2= MlvlV2FPT0WU
SK-PN-DW NY\wWFRIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGfzempKSzVyPUCuNlMyQSEQvF2= MnPNV2FPT0WU
HD-MY-Z M2fnfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWjwZ2xuUUN3ME2wMlI{OzB|IN88US=> MUPTRW5ITVJ?
LB373-MEL-D NH\5cIJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWrpboczUUN3ME2wMlI1OTl6IN88US=> M1TFdHNCVkeHUh?=
COLO-829 MoXlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{C5R2lEPTB;MD6yOFI2PyEQvF2= M1PSbnNCVkeHUh?=
ES8 NGjvdIhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFfyZY5KSzVyPUCuNlQ4PjNizszN MkTIV2FPT0WU
RXF393 M{fqVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1;JO2lEPTB;MD6yOVAyPSEQvF2= MYjTRW5ITVJ?
TK10 Mo\rS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVfx[3hnUUN3ME2wMlI2PDN3IN88US=> MXnTRW5ITVJ?
LOUCY MnzlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXfkWlVbUUN3ME2wMlI2PDV4IN88US=> M3zVXXNCVkeHUh?=
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NCI-H1882 MnixS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFPQbmhKSzVyPUCuOVkxOzZizszN NVvhNW9GW0GQR1XS
TE-9 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYHJR|UxRTBwNkGwN|Mh|ryP MV7TRW5ITVJ?
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SW872 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NULSWmxtUUN3ME2wMlY2PzJ7IN88US=> MmLBV2FPT0WU
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NCI-H719 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4TURmlEPTB;MT6yO|QyOSEQvF2= M{jwNnNCVkeHUh?=
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NCI-H128 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn\kTWM2OD1zLkO1PFE1KM7:TR?= MXLTRW5ITVJ?
HC-1 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUPJR|UxRTFwM{ixO{DPxE1? NY[4OmlbW0GQR1XS
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DMS-114 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoDHTWM2OD1zLkS0OVM6KM7:TR?= M2rte3NCVkeHUh?=
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NCI-H2081 NXHTbIZDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3TIbmlEPTB;MT61OVE6PiEQvF2= NGCwT4ZUSU6JRWK=
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NCI-H209 Mnz5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGPLRoRKSzVyPUWuNVk{PTJizszN M{XmXnNCVkeHUh?=
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NCI-H1581 M2XWSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mm\jTWM2OD14LkKzPFQ6KM7:TR?= MnPTV2FPT0WU
HUTU-80 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUnJR|UxRTZwM{[4PVch|ryP M4XW[XNCVkeHUh?=
TGW MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mkf3TWM2OD14LkS1OVk{KM7:TR?= NETrSZlUSU6JRWK=
SK-N-FI M1XqSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYXJR|UxRTZwNEW4OVQh|ryP M{\ldXNCVkeHUh?=
U-266 MnLjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHHRe5pKSzVyPU[uOVE6OjZizszN NWnsbY8yW0GQR1XS
EM-2 NFzlZmZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWDJR|UxRTZwNkewPVkh|ryP NXK2TY1uW0GQR1XS
NMC-G1 NVziWIFxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXm4SWRkUUN3ME22MlcyQDl3IN88US=> MmrNV2FPT0WU
KASUMI-1 MnLwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlHwTWM2OD14Lki0O|g4KM7:TR?= NGDXO4ZUSU6JRWK=
NALM-6 NFS3eIdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M13WcWlEPTB;Nj64Olc2PCEQvF2= M3v2NHNCVkeHUh?=
OCI-AML2 MmHvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEfxendKSzVyPUeuNFA{OjZizszN NWGzfpdYW0GQR1XS
SHP-77 M4HvR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlXCTWM2OD15LkKyOFMh|ryP NHXpN4JUSU6JRWK=
NOMO-1 Ml7ZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2LEfWlEPTB;Nz6yOFI2PCEQvF2= Mk\YV2FPT0WU
SK-N-DZ Mne5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmTaTWM2OD15LkexNFg6KM7:TR?= M2[xWXNCVkeHUh?=
LB1047-RCC NUHTcYpoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUTJR|UxRTdwN{KyN|Eh|ryP NHTzW2RUSU6JRWK=
MZ1-PC MoXRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NInFeZRKSzVyPUeuPFY2PThizszN MUjTRW5ITVJ?
NB10 NIjvS5dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVnKNW1WUUN3ME23Mlk6PTV4IN88US=> NXn2bWhHW0GQR1XS
RL95-2 NEDDcJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXLUTJVbUUN3ME24MlExQDR{IN88US=> NFTzSotUSU6JRWK=
OMC-1 NVjzb2tmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1f0fmlEPTB;OD61NlE6OSEQvF2= MoXXV2FPT0WU
D-283MED M1TYSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF\sRWZKSzVyPUiuPVE4OTlizszN M1\aNnNCVkeHUh?=
MC116 MoX2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1O4[GlEPTB;OD65O|k1KM7:TR?= MY\TRW5ITVJ?
SJSA-1 NYK0T|g2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXj0ZYJOUUN3ME25MlA6ODlzIN88US=> MYPTRW5ITVJ?
JiyoyeP-2003 MmO5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYnTRYR5UUN3ME25MlI6OzF5IN88US=> MoDaV2FPT0WU
IST-MEL1 NHLkO3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXLJR|UxRTlwN{SxPVgh|ryP MWrTRW5ITVJ?
CTV-1 M1KxRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MY\JR|UxRTFyLkC5O|kh|ryP M2D1eXNCVkeHUh?=
NH-12 NWDPfnhMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Moq2TWM2OD1zMD6yOFM{KM7:TR?= NI\C[VJUSU6JRWK=
CA46 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVXJR|UxRTFyLkO2NUDPxE1? M{DjVXNCVkeHUh?=
NCI-SNU-1 NWj2UGl1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVn6eGR[UUN3ME2xNE41QTZ7IN88US=> NE\FcFJUSU6JRWK=
SCLC-21H NUKySpZGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGDFbohKSzVyPUGwMlY2QTZizszN M33Qe3NCVkeHUh?=
EC-GI-10 NFzmU49Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFzNUmJKSzVyPUGwMlcxOzFizszN M{C4THNCVkeHUh?=
SR NHvjZ2pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MonWTWM2OD1zMT6wNVg2KM7:TR?= M4jNZXNCVkeHUh?=
NCI-H1648 NXfXeYVET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWniOYhOUUN3ME2xNU4xQTZ3IN88US=> NWG5cHZkW0GQR1XS
TGBC1TKB MlvhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFzlT4ZKSzVyPUGxMlQyODJizszN MWjTRW5ITVJ?
EW-11 Mn24S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXS0cI1YUUN3ME2xNU42OTh4IN88US=> M1zKOXNCVkeHUh?=
SK-MM-2 Mor4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYG2[Y9OUUN3ME2xNU45ODZzIN88US=> MXnTRW5ITVJ?
NCI-H524 NInqS41Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXrJR|UxRTFzLkm4NlIh|ryP MYLTRW5ITVJ?
NOS-1 MniyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH;TfoJKSzVyPUGyMlA{PDVizszN MmTrV2FPT0WU
AM-38 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV25UmlmUUN3ME2xNk42PjN|IN88US=> MYPTRW5ITVJ?
A498 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV3JR|UxRTF{LkewOlkh|ryP M3TqWXNCVkeHUh?=
KARPAS-422 NH3zT2JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmjKTWM2OD1zMj63OFk3KM7:TR?= NFXW[FZUSU6JRWK=
LU-139 NXy4Oo5jT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIj0S4xKSzVyPUGyMlkxOjZizszN M{LVSHNCVkeHUh?=
COR-L88 NF3ZSWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXLJR|UxRTF{LkmzPFIh|ryP MmTXV2FPT0WU
K5 Mmf6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXzJR|UxRTF{Lkm0OlIh|ryP NITxdZJUSU6JRWK=
NB13 NIrufIpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnLwTWM2OD1zMj65O|g{KM7:TR?= MX\TRW5ITVJ?
MRK-nu-1 NF:xXVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoLxTWM2OD1zMz6xPVUh|ryP NUfhWW1PW0GQR1XS
MHH-NB-11 MmDYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFznSXhKSzVyPUGzMlI6ODJizszN MmjKV2FPT0WU
KU812 NYmzdnRkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVHmToJKUUN3ME2xN{43OTJ2IN88US=> MXjTRW5ITVJ?
TE-12 NVSx[3JKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYXJR|UxRTF|Lk[5PVQh|ryP NU\k[GVwW0GQR1XS
NCI-N87 MkHWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGKySnpKSzVyPUGzMlc{PiEQvF2= MoLvV2FPT0WU
EB2 NEHuNIRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NI[2Zo9KSzVyPUGzMlg2ODVizszN MW\TRW5ITVJ?
DB Mn\0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXzJR|UxRTF|Lkm4PFUh|ryP M4LsS3NCVkeHUh?=
697 NEnhZ5ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYXJR|UxRTF2LkS1NVEh|ryP Mm\jV2FPT0WU
MSTO-211H MonkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV3PfmxTUUN3ME2xOE44PDR6IN88US=> MonKV2FPT0WU
JVM-2 MmPNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVLJR|UxRTF2Lke3N|Uh|ryP NW\Mc5FzW0GQR1XS
COLO-824 NHjGe4NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXnJR|UxRTF2Lke5PVQh|ryP M{jjPHNCVkeHUh?=
BC-3 MkHZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1:zUmlEPTB;MUWuN|k5KM7:TR?= NYLlXldEW0GQR1XS
BOKU MoS5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3izXGlEPTB;MUWuPVYxQCEQvF2= M{DuUHNCVkeHUh?=
GOTO NH22VZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVXJR|UxRTF4Lkm2NVch|ryP NWe2RY9ZW0GQR1XS
HCC2157 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NILUb2ZKSzVyPUG3MlQ4PTVizszN MXjTRW5ITVJ?
LS-1034 M1;xU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVXZe4N7UUN3ME2xO{43QDF6IN88US=> NED5cVJUSU6JRWK=
CAL-148 Ml\kS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXHJR|UxRTF5Lkm3PVYh|ryP MknoV2FPT0WU
MOLT-4 M3Oy[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVTJR|UxRTF6LkiyNFgh|ryP NGfkfmxUSU6JRWK=
Daudi MlnPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoHRTWM2OD1zOD65NlMyKM7:TR?= MVXTRW5ITVJ?
J-RT3-T3-5 M1KwOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3flR2lEPTB;MUmuOFA3PiEQvF2= NYfJemN1W0GQR1XS
KMOE-2 NHW5NJBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX7FR|R4UUN3ME2xPU43PDh{IN88US=> M4rsXnNCVkeHUh?=
HL-60 M124XWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF34VmJKSzVyPUKwMlE6PjVizszN M{fFbnNCVkeHUh?=
P31-FUJ NFXVWGpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUXWNWhJUUN3ME2yNE41PzV|IN88US=> M1r0VnNCVkeHUh?=
IM-9 MnX0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX3JR|UxRTJyLk[yNlQh|ryP M4fqW3NCVkeHUh?=
HDLM-2 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHHBWIZKSzVyPUKwMlg5OjFizszN M4TLRnNCVkeHUh?=
NCI-H1304 NEjsUG1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXz1e|VYUUN3ME2yNU4xOzFzIN88US=> M3vL[HNCVkeHUh?=
NCI-H345 M4fBSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWjzdWpGUUN3ME2yNU4xPjR|IN88US=> MmnKV2FPT0WU
RPMI-6666 NVfjPIRsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXrxfVFFUUN3ME2yNU4{PjB{IN88US=> M{nnWXNCVkeHUh?=
GR-ST MmnqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWHJR|UxRTJzLkSxO{DPxE1? NYHuR|lPW0GQR1XS
CHP-126 M4TiTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1qwbGlEPTB;MkGuOlE5OSEQvF2= NUHlb|hSW0GQR1XS
EHEB NFLWN3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3i2NmlEPTB;MkGuOlc{OSEQvF2= NY\pUYxXW0GQR1XS
CPC-N NWDWe5NvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXXKSYdqUUN3ME2yOE4xOjBzIN88US=> NVLFU4ZLW0GQR1XS
NB1 NVXNUo5DT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnXSTWM2OD1{ND62PVM6KM7:TR?= NVnFeGpnW0GQR1XS
LS-123 NI\k[WdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIXIblFKSzVyPUK0MlkxOTJizszN M1TPbXNCVkeHUh?=
ST486 M2jtU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXHJR|UxRTJ3LkGxNVMh|ryP MkjnV2FPT0WU
NCI-H1963 NF2xfIdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYe2Z5JJUUN3ME2yOk4xPjJ|IN88US=> M37URnNCVkeHUh?=
U-87-MG NHfRXHZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUjJR|UxRTJ4Lke2OFQh|ryP MYHTRW5ITVJ?
COR-L279 NXz1[ZpTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnrwTWM2OD1{Nj63PVIzKM7:TR?= NXTnUWo5W0GQR1XS
LU-165 NXXldFBMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mlu2TWM2OD1{OD6wPFYyKM7:TR?= MkHMV2FPT0WU
COLO-800 Mkn4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXrq[Fc2UUN3ME2yPE4zQTV4IN88US=> MV;TRW5ITVJ?
ETK-1 NVexeY1PT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1zQRmlEPTB;MkiuN|Q1PiEQvF2= NF3UOnRUSU6JRWK=
LNCaP-Clone-FGC NELBfZZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXjvTlVVUUN3ME2yPU46OjhzIN88US=> MoXVV2FPT0WU
SIG-M5 NWmwW3hyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYjjNI43UUN3ME2zNE44QTV{IN88US=> NUCzZ5lpW0GQR1XS
NB6 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXL6WVZDUUN3ME2zNE46PTB|IN88US=> Mke5V2FPT0WU
NCI-H2107 M2\pPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MonlTWM2OD1|MT6yN|M5KM7:TR?= MoHpV2FPT0WU
SNU-C1 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFLzVGZKSzVyPUOxMlMyOjZizszN M3zYWXNCVkeHUh?=
JAR M3\IbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWrTXokzUUN3ME2zNU4{Pzl7IN88US=> M13kNXNCVkeHUh?=
L-363 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYf5SYR1UUN3ME2zNk4zOTR2IN88US=> NFTTe2RUSU6JRWK=
EW-24 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUDJR|UxRTN{LkO0O|Qh|ryP Ml22V2FPT0WU
NB69 NXPjSnU{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4jUTmlEPTB;M{OuOlQ2PCEQvF2= MW\TRW5ITVJ?
EW-13 M1;EWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2TzdWlEPTB;M{OuPFk3QSEQvF2= NHrxUZlUSU6JRWK=
Ramos-2G6-4C10 NGf1WIxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlfYTWM2OD1|ND6yPVk{KM7:TR?= NHe4PZZUSU6JRWK=
TE-11 MlzhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlrzTWM2OD1|ND60PFIzKM7:TR?= NEHuXGFUSU6JRWK=
L-428 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{S2eWlEPTB;M{SuO|U{PiEQvF2= MXrTRW5ITVJ?
KP-N-YN MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYX5OmFGUUN3ME2zOE46ODV5IN88US=> M1zxXXNCVkeHUh?=
CGTH-W-1 MlmwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUizZW9zUUN3ME2zOk46ODR6IN88US=> NUnHdldWW0GQR1XS
K-562 NHzqN3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGDJPIpKSzVyPUO3MlA5PjRizszN MXzTRW5ITVJ?
NCI-H1299 MmnYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVrJR|UxRTN6LkG2NFMh|ryP MXfTRW5ITVJ?
RCC10RGB NXXrcYZ[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2L6d2lEPTB;M{iuNlA5OyEQvF2= M2HhcnNCVkeHUh?=
NCI-SNU-16 MlfES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MofWTWM2OD1|OD61OlU{KM7:TR?= MULTRW5ITVJ?
LC-2-ad NFrxW4NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2HmNmlEPTB;M{muOFM{PyEQvF2= M3fsTXNCVkeHUh?=
MHH-PREB-1 NYjseY44T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHfVTnRKSzVyPUO5Mlc{QDhizszN NV7o[Wh1W0GQR1XS
NCI-H64 NWHhfZVRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2\oRmlEPTB;NECuNFk1QCEQvF2= MXTTRW5ITVJ?
LB996-RCC Mlr2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmnhTWM2OD12MD64O|E3KM7:TR?= MnPUV2FPT0WU
DEL NWLTZoZVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmLNTWM2OD12MT60OFA2KM7:TR?= NFzEb2FUSU6JRWK=
MLMA NY\Td3RET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVr1XYplUUN3ME20NU44PTl3IN88US=> MofRV2FPT0WU
SBC-1 NHP6dW1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYP6PG9IUUN3ME20Nk4yQDB4IN88US=> Ml;vV2FPT0WU
MPP-89 MkPCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3HIZWlEPTB;NEKuOVg4PSEQvF2= NIjqc2lUSU6JRWK=
MV-4-11 NWjWUnhTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVXyVlRPUUN3ME20Nk46ODZ5IN88US=> M1zp[HNCVkeHUh?=
EoL-1- Mnz6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV\JR|UxRTR2LkG1Olgh|ryP MUfTRW5ITVJ?
CW-2 MoDZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFTBT|NKSzVyPUS0Mlg{PDJizszN M4\kRXNCVkeHUh?=
HT MmHsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NITHSIJKSzVyPUS1MlcxODdizszN MljDV2FPT0WU
SW954 M{nFcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV7JR|UxRTR5LkSxNlgh|ryP M4DySHNCVkeHUh?=
A3-KAW NInDfYhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVX1fpZIUUN3ME20PU45ODZzIN88US=> MoiyV2FPT0WU
TC-YIK MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3;0WmlEPTB;NUCuNFM3OyEQvF2= MWfTRW5ITVJ?
SW962 NEnWOHlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEPoWnJKSzVyPUW0Mlg{PTdizszN M2fSS3NCVkeHUh?=
KP-N-RT-BM-1 MmTNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGfTTlhKSzVyPUW2MlY3OzhizszN NXXVe5N5W0GQR1XS
NCI-H1395 MlPiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3XoS2lEPTB;NUiuPFE4OiEQvF2= MUPTRW5ITVJ?
RPMI-8402 MlLwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{XUXGlEPTB;NUiuPVUzPiEQvF2= M1TPdnNCVkeHUh?=
SCH MonGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWfJR|UxRTZyLkm2N|gh|ryP MojJV2FPT0WU
NCI-H2196 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHT6dJNKSzVyPU[xMlI{OSEQvF2= MXzTRW5ITVJ?
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... Click to View More Cell Line Experimental Data

In vivo Administration of MG-132 effectively rescues the expression levels and plasma membrane localization of dystrophin, β-dystroglycan, α-bdystroglycan, and α-sarcoglycan in skeletal muscle fibers from mdx mice, reduces muscle membrane damage, and ameliorates the histopathological signs of muscular dystrophy. [7] MG-132 treatment significantly reduces immobilization-induced skeletal muscle atrophy in mice, by downregulating the muscle-specific ubiquitin ligases atrogin-1/MAFbx and MuRF-1 mRNA. [8]

Protocol

Kinase Assay:

[1]

+ Expand

Measurement of inhibitory activities of MG-132 against 20S proteasome:

The reaction mixture for the 20S proteasome inhibitory assay contains 0.1 M Tris-acetate, pH 7.0, 20S proteasome, MG-132, and 25 μM substrate dissolved in dimethyl sulfoxide in a final volume of 1 mL. After incuba tion at 37 °C for 15 minutes, the reaction is stopped by the addition of 0.1 mL of 10% SDS and 0.9 mL of 0.1M Tris acetate, pH 9.0. The fluorescence of the reaction products is measured. To determine the IC50 against 20S proteasome, various concentrations of MG-132 are included in the assay mixture.
Cell Research:

[3]

+ Expand
  • Cell lines: KIM-2, HC11, and ES
  • Concentrations: Dissolved in DMSO, final concentrations ~25 μM
  • Incubation Time: 24, and 48 hours
  • Method:

    Cells are exposed to various concentrations of MG-132 for 24, and 48 hours. Supernatant and monolayer cells are harvested by centrifugation and fixed in 70% ethanol in PBS for staining with acridine orange. Equal volumes of cells and acridine orange (5 mg/mL in PBS) are mixed on a microscope slide and examined by fluorescence microscopy. For annexin V analysis, cells are harvested by centrifugation and stained with annexin V and propidium iodide. For cell cycle analysis, cells are rehydrated in PBS at room temperature for 10 minutes, followed by staining with propidium iodide (5 mg/mL). All samples are analyzed using a Coulter Epics XL flow cytometer.


    (Only for Reference)
Animal Research:

[7]

+ Expand
  • Animal Models: Male mdx (C57BL/10ScSn DMD mdx) mice
  • Formulation: Dissolved in DMSO, and diluted in PBS
  • Dosages: ~10 μg/kg/day
  • Administration: Injection
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 95 mg/mL (199.73 mM)
Ethanol 95 mg/mL (199.73 mM)
Water <1 mg/mL
In vivo 4% DMSO+30% PEG 300+20% propylene glycol+ddH2O 2mg/mL

* 1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 475.62
Formula

C26H41N3O5

CAS No. 133407-82-6
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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  • Computed Result

  • C1=C0/X C1: LOG(C1):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID