MG-132

Catalog No.S2619

MG-132 Chemical Structure

Molecular Weight(MW): 475.62

MG132 is a potent cell-permeable proteasome and calpain inhibitor with IC50s of 0.1 and 1.2 μM for the inhibition of proteasome and calpain, respectively.

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Selleck's MG-132 had a noticeably greater effect when compared with similar products from Sigma: This is directly due to using the (S, R, S) - (-) - 2 configuration.

P21 protein can be degraded through ubiquitylation pathway. Inhibition of proteasome activities results in the accumulation of P21 protein, therefore, the more powerful the inhibiting effect, the more P21 protein is accumulated. (testing protein: P21; Cell line: Hela; treatment condition: final concentration of MG-132 is 5μM, with a 6 hour treatment time)

Cited by 48 Publications

11 Customer Reviews

  • Western blot of IκBζ expression in BMDMs treated with DI and stimulated with LPS for 1 h. MG132 or bafilomycin A (BafA) were added 30 min before LPS stimulation.

    Nature, 2018, 556(7702):501-504. MG-132 purchased from Selleck.

    MDA-MB-231 cells were treated with 10 uM MG132 and incubated under normoxia or hypoxia for 4 h. Endogenous interaction between LATS2 and SIAH2 was analysed by immunoprecipitation.

    Nat Cell Biol 2015 17(1), 95-103. MG-132 purchased from Selleck.

  • HeLa cells were transfected with control mimic, RMND5A siRNA or miR-138 mimic. After transfection for 36 h, cells were treated with proteasome inhibitor MG132 (20 uM) or Dimethyl sulfoxide (DMSO) for 8 h. Endogenous Exportin-5 protein and RMND5A protein expression was measured by western blotting.

    Nucleic Acids Res 2014 42(1), 458-74. MG-132 purchased from Selleck.

    HeLa cells were transfected with negative control mimic, miR-138 mimic or RMND5A siRNA. After transfection for 36 h, cells were treated with MG132 (20 uM) or DMSO for 8 h. RMND5A and Exportin-5 protein expression were analyzed by immunocytochemistry. Nuclei were counterstained with DAPI.

    Nucleic Acids Res 2014 42(1), 458-74. MG-132 purchased from Selleck.

  •  

    TFEB induced autophagy dependent ATZ clearance in vitro. MEF transiently co-transfected with either ATZ plus GFP expressing plasmids or ATZ plus TFEB expressing plasmids were incubated with proteasome inhibitor MG132 or with DMSO for 6 h. Upon MG132 treatment, steady state levels of 52 kDa ATZ band increased in both cells transfected with ATZ plus GFP and with ATZ plus TFEB expressing plasmids. Quantification of band intensities showed a 48% reduction of ATZ in cells transfected with ATZ plus TFEB compared to cells transfected with ATZ plus GFP, in the absence of the proteasome inhibitor. In MG132 treated cells, a 61% decrease of ATZ was detected in cells transfected with ATZ plus TFEB compared to cells transfected with ATZ plus GFP.

    EMBO Mol Med 2013 5, 397-412. MG-132 purchased from Selleck.

    JAK1 expression was analyzed in A375 cells expressing scrambled shRNA or shRNF125 (#2) and treated with MG132 (5 uM, 10 hr), and in Lu1205 cells expressing WT RNF125 and treated with MG132.

    Cell Rep 2015 11(9), 1458-73. MG-132 purchased from Selleck.

  • LRB1 and LRB2 regulate phyB protein levels. PhyB protein levels in crude extracts from the wild type (WT) and the indicated mutant backgrounds were assayed by immunoblot analysis with anti-phyB monoclonal antibodies. Anti-PBA1 and anti-histone H3 antibodies were used to confirm equal protein loading. Rc-induced loss of phyB in the wild type is inhibited by 100 uM MG132.

    Plant Physiol 2012 60(1), 118-34. MG-132 purchased from Selleck.

    Representative images for γ-H2AX-stained cells treated with 100 uM FA for 3 h without or with MG132. Fluorescence was measured in at least 50 nuclei in each of three experiments.

    Toxicol Appl Pharmacol 2015 286(2), 135-41. MG-132 purchased from Selleck.

  • GSK-3β phosphorylation at Ser9 is enhanced in NOK-overexpressed stable cell lines. The proteasome inhibitor, MG132, was added in medium of HeLa-HA and HeLa-NOK-HA cells for 12 h and then the p-GSK-3b(Ser9) was assayed by western blotting.

    FEBS Lett 2012 586, 3787–3792. MG-132 purchased from Selleck.

    Nek2A impedes SuFu ubiquitination. HEK293T cells transfected with Nek2A-Myc and Flag-SuFu for 48 h were treated with MG-132 to enrich ubiquitinated proteins, and subsequently subjected to co-immunoprecipitation assay. Precipitated proteins were probed using anti-Ub antibody.

    Int J Oncol, 2017, 50(2):373-380. MG-132 purchased from Selleck.

  • Truncated mouse Period2 (Per2) protein was expressed in NIH3T3 cells, treated by cycloheximide with or without MG132 for 1-4 hours.Per2 was reported to be degraded by the proteasome proteolysis. By adding MG132 (25 μM), a proteasome inhibitor, the degradation of Per2 was dramatically decreased within 4 hours treatment (shown by western blot).

    2012 Dr. Edison DUKE-NUS Graduate Medical School Singapore.. MG-132 purchased from Selleck.

Purity & Quality Control

Choose Selective Proteasome Inhibitors

Biological Activity

Description MG132 is a potent cell-permeable proteasome and calpain inhibitor with IC50s of 0.1 and 1.2 μM for the inhibition of proteasome and calpain, respectively.
Targets
20S proteasome [9]
(Cell-free assay)
100 nM
In vitro

MG-132 displays >1000 times more activity than ZLLal in inhibiting the ZLLL-MCA-degrading activity of 20S proteasome with IC50 of 100 nM versus 110 μM. MG-132 also inhibits calpain with IC50 of 1.2 μM. MG-132 induces neurite outgrowth in PC12 cells at an optimal concentration of 20 nM, displaying 500 times more potency than ZLLal. [1] MG-132 (10 μM) potently inhibits TNF-α-induced NF-κB activation, interleukin-8 (IL-8) gene transcription, and IL-8 protein release in A549 cells by inhibition of proteasome-mediated IκBα degradation. [2] MG-132 treatment potently induces p53-dependent apoptosis in KIM-2 cells by 26S proteasome inhibition. [3] Unlike BzLLLCOCHO or PS-341, MG-132 treatment results in weak inhibition of the chymotrypsinlike (CT-L) and peptidylglutamyl peptide hydrolysing (PGPH) activities of the 26S proteasome, whereas multiple myeloma cells (U266 and OPM-2) are more sensitive to induction of apoptosis by MG-132 than BzLLLCOCHO. [4] MG-132 (1 μM) sensitizes TRAIL-resistant prostate cancer cells by activating the AP-1 family members c-Fos and c-Jun, which, in turn, repress the antiapoptotic molecule c-FLIP(L). [5] MG-132 significantly enhances the ability of inositol hexakisphosphate (IP6) to reduce cellular metabolic activity in both PC3 and DU145 androgen-independent prostate cancer (AIPCa) cell lines. [6]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LPS-stimulated RAW264.7 cells NY[0PZZsTnWwY4Tpc44hSXO|YYm= NWPlSVZUOjVizszN M{jGZWROW09? Mm\3TY5pcWKrdIOgcol1emmlIH;4bYRmKHC{b3T1Z5Rqd25id3n0bEBKSzVyIH;mJFAvOSEQvF2= MV6yOFY6PzR7Nh?=
HL-60 NHLYR3dEgXSxdH;4bYMhSXO|YYm= NUHsSo9VPDBizszN NHf3R481QCCq NUTF[ZF1TE2VTx?= MojmTWM2ODxzMDFOwG0> M{D5OVI1Pjl5NEm2
SMMC-7721 MVfDfZRwfG:6aXOgRZN{[Xl? NYX2OWZRPDBizszN NWfXVJl4PDhiaB?= NX[yVot1TE2VTx?= NXnufZA5UUN3ME23MlEh|ryP NF7yVIMzPDZ7N{S5Oi=>
A-549 NWDUN4l1S3m2b4TvfIlkKEG|c3H5 NYHBSlk5PDBizszN M{TwWFQ5KGh? NYrrUnhkTE2VTx?= M{TXeWlEPTB:MUCg{txO NUXxbY1NOjR4OUe0PVY>
MCF-7 M2HUT2N6fG:2b4jpZ{BCe3OjeR?= MoLHOFAh|ryP NFj3[Gw1QCCq NXfhdI5CTE2VTx?= M2HzVmlEPTB;Nz6zJO69VQ>? NEDLeHUzPDZ7N{S5Oi=>
SW-480 NGnsVGxEgXSxdH;4bYMhSXO|YYm= M4jWWlQxKM7:TR?= Ml;rOFghcA>? MWLEUXNQ MlfxTWM2OD12IN88US=> NETCO4UzPDZ7N{S5Oi=>
NCI-H929 NYj0[GZ1S3m2b4TvfIlkKEG|c3H5 NYrkWZZQOSEQvF2= MXS3NkBp MmX2SG1UVw>? NVPHOmxKUUN3ME2wMlE4KM7:TR?= NVuzV2JUOjR4MkWwPFg>
293T NFH2VHREgXSxdH;4bYMhSXO|YYm= NWXZeWI{OTBizszN M3nJeFczKGh? NVvvNY9OTE2VTx?= NU\ZTHZRUUN3MEyyJO69VQ>? MoK1NlQ3OjVyOEi=
293T NXHofZZ6TnWwY4Tpc44hSXO|YYm= NUP1ZoFZOTBizszN M{nZOFI1KGh? M1vHd2ROW09? MV7Nc4RmemG2ZXz5JIlv\HWlZYOgbIVifC2|aH;jb{Ah MkjsNlQ3OjVyOEi=
HeLa NH34fWtMcW6jc3WgRZN{[Xl? MYCxNEDPxE1? NV\ZPWpoOSCq MoC0SG1UVw>? MlL6TY5lfWOnczDQRXJRKGOuZXH2ZYdmKGK7IHnubIljcXSrbnegdJJwe2Wjc3;t[UBi[3Srdnn0fS=> NHjWXWQzPDN{MUizNy=>
MDA-MB-231 NVLq[oNTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2PHWlEh|ryP NUD6XGZKPzJiaB?= MXLEUXNQ MlW4TWM2OD1yLkG4JO69VQ>? NFjKUJYzPDF3M{KwOi=>
MCF-7 Mo[4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFTGeW0yKM7:TR?= NGHwdXg4OiCq NUjjOIFXTE2VTx?= M4PVRmlEPTB;MD6xN{DPxE1? MmTGNlQyPTN{ME[=
MCF10A MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWDKT3loOSEQvF2= MWK3NkBp NFXLNWlFVVOR M1XBTWlEPTB;MD6yPUDPxE1? MkXFNlQyPTN{ME[=
HEK-293 NFTEZ|JMcW6jc3WgRZN{[Xl? NUfCPFBNOiCjbnSgNlAh|ryP NIHZblgzKGh? MkDCSG1UVw>? NIPIWHVKdmirYnn0d{BEcFRvTDDhZ5Rqfmm2eTD3bZRpKEmFNUCgc4YhQSCwTTC= MnXnNlM2PDB5OUC=
Calu6 M3vJcWZ2dmO2aX;uJGF{e2G7 NIDN[YMyOCEQvF2= NGDwOYQyQCCq NULNSlM6TE2VTx?= NUnRcm44W2mpbnnmbYNidnSueTDhZ4N2dXWuYYTld{BnemG2YYjpckBxemWldYLzc5I> MlTlNlM2ODZ2OE[=
IFN-gamma-induced RAW264.7 NI\Udo9HfW6ldHnvckBCe3OjeR?= NWnyWpJxTE2VTx?= NIWxXHRKdmirYnn0d{BvcXS{aXOgc5hq\GVicILv[JVkfGmxbjD3bZRpKEmFNUCgc4YhOC5yN{eg{txO NWXQdmNnOjJ{N{eyO|c>
IPC227F NEG2e|dEgXSxdH;4bYMhSXO|YYm= MV[xJO69VQ>? MnTJOFghcA>? MYHEUXNQ M1XRS2lEPTB;MD6wO|ch|ryP NX35U4hVOjF7MkS1Nlg>
Hepa-1c1c7 MofwSpVv[3Srb36gRZN{[Xl? MX2yOUDPxE1? NX\xRYRCPiCq NWn5NHU4TE2VTx?= Mn7QTY5kemWjc3XzJG5z\jJicILveIVqdiCuZY\lcC=> NWnDZ4VpOjB|OUK1OFQ>
COS-7 M{TUT2N6fG:2b4jpZ{BCe3OjeR?= NH;Lc20yOCEQvF2= MoWxSG1UVw>? M3XqbmlEPTB:MUCg{txO MWexPFA5QDB7Nx?=
HuH-7 NEHVT|lEgXSxdH;4bYMhSXO|YYm= M3;rWlExKM7:TR?= MYDEUXNQ NX3yWYIyUUN3MEyxNEDPxE1? MlXXNVgxQDhyOUe=
OCI-Ly3 NH7Jeo5HfW6ldHnvckBCe3OjeR?= NFvLSpAyOCEQvF5CpC=> MlroOEBp NWPIe4pwTE2VTx?= MlnBTY5lfWOnczDoeY1idiCLa3HwdIFD[WyyaHGgd5Ri[mmuaYrheIlwdiC5aYToJGVEPTBib3[gNUDPxE1? M1;hdFE5ODJ2MUGz
A2780 cDDP NY\WOnVxSXCxcITvd4l{KEG|c3H5 NFj0PVUzPCCq NUnHNpZ5TE2VTx?= M4nCd2lv\HWlZYOgZZBweHSxc3nzJIJ6KGmwaHnibZRqdmdiUGTFUkBl\We{YXTheIlwdg>? MVKxO|Y5PDBzOB?=
LPS-stimulated RAW264.7 cells NHK2WIJHfW6ldHnvckBCe3OjeR?= MV7EUXNQ NGWzdm5KdmirYnn0d{BPTi2tYYDwZWIhTE6DIHLpcoRqdmd? Mn\VNVc1ODd{N{e=
PC12 MX;GeY5kfGmxbjDBd5NigQ>? MWixNFAh|ryPwrC= NXG1[m8yOjRiaB?= M1Tj[GROW09? MmezTY5pcWKrdIOgOk1QUESDLTDhcoQhUDKRMj3pcoR2[2WmIHP5eI91d3irY3n0fS=> M13UNlE4OTV6NEW0
PC3 NHy5bJRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV7xS21kOjBizszN MXS0PEBp Mo\ESG1UVw>? NG\JeXBKSzVyPUCuOkDPxE1? NYfnWYFkOTZ4OE[1N|c>
LP-1 NHLmdZRCeG:ydH;zbZMhSXO|YYm= NFuy[Vg{ODBibl2= MYCyOEBp NHnicodFVVOR NXrNUJlTUW6mdXPld{BieG:ydH;zbZMh[nliaX7jdoVie2mwZzDjcIVifmWmIGDBVnAhdGW4ZXygZY5lKHKnZIXjbY5oKE2lbD2xJJBzd3SnaX6gcIV3\Wx? NFTGbWwyPTl3OEW4PS=>
ES6 M17hc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1;TUmlEPTB;MD6wNVMxPiEQvF2= NGDvVHNUSU6JRWK=
A101D MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYLqfHRmUUN3ME2wMlA{Ojd7IN88US=> Mni4V2FPT0WU
OCUB-M M3PUcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGPJclRKSzVyPUCuNFM4OzdizszN M17QbnNCVkeHUh?=
LB2518-MEL M4nlO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlP5TWM2OD1yLkCzO|Yh|ryP MVfTRW5ITVJ?
SH-4 Ml;tS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml7vTWM2OD1yLkC0N|Eh|ryP MknFV2FPT0WU
KNS-42 MkDXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXzJR|UxRTBwMES0OFEh|ryP Mlf2V2FPT0WU
DSH1 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnjBTWM2OD1yLkC1Nlg6KM7:TR?= NISx[WJUSU6JRWK=
NTERA-S-cl-D1 MofUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2WweWlEPTB;MD6wOVc4OiEQvF2= MXLTRW5ITVJ?
D-542MG NXLlVHpzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MY\JR|UxRTBwMEW5OVch|ryP NEDyfJFUSU6JRWK=
KS-1 MnfpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIHMXlFKSzVyPUCuNFY1PThizszN MYHTRW5ITVJ?
BL-41 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3PtUWlEPTB;MD6wOlk1PyEQvF2= MWPTRW5ITVJ?
LXF-289 NHzBUWpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoDZTWM2OD1yLkC3NFY3KM7:TR?= NHXQOnNUSU6JRWK=
D-247MG MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX7JR|UxRTBwMEeyNFkh|ryP NE\zSmRUSU6JRWK=
MMAC-SF NGnqUFNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYrJR|UxRTBwMEeyOlgh|ryP NH25WZlUSU6JRWK=
CP66-MEL NFnjeXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUTJR|UxRTBwMEe1JO69VQ>? MVvTRW5ITVJ?
LB771-HNC Ml;iS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVnJR|UxRTBwMEiwN|Yh|ryP MWPTRW5ITVJ?
no-10 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlTNTWM2OD1yLkC4PVA6KM7:TR?= M37rPXNCVkeHUh?=
A388 MmDyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUOzT|VTUUN3ME2wMlA6ODZ3IN88US=> M330VnNCVkeHUh?=
OPM-2 NVf6XI5kT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3TLeWlEPTB;MD6xNFQ4PCEQvF2= M1TYfXNCVkeHUh?=
OVCAR-4 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2PoNmlEPTB;MD6xNFk{QCEQvF2= NYLOfld{W0GQR1XS
HOP-62 MmW2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX71TYg2UUN3ME2wMlExQTR7IN88US=> NV\lRoo6W0GQR1XS
ML-2 NGHBSFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWXTNphnUUN3ME2wMlEyQDB4IN88US=> MYXTRW5ITVJ?
UACC-257 MkHIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M37NNWlEPTB;MD6xNVkzPyEQvF2= NXPjcZlxW0GQR1XS
NEC8 M3XVcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYT5c2FHUUN3ME2wMlEyQTl3IN88US=> NELrPVRUSU6JRWK=
ONS-76 NWX1cZF{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4PmfGlEPTB;MD6xNlk{PSEQvF2= NUCxUXBTW0GQR1XS
KE-37 MkSzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIjVS2lKSzVyPUCuNVMzPzhizszN NWfW[ZRLW0GQR1XS
HT-144 NXzOW3AyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3rEdWlEPTB;MD6xN|g6PSEQvF2= MVjTRW5ITVJ?
LB2241-RCC MoT0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXLJR|UxRTBwMUSyOFch|ryP NEjvS4pUSU6JRWK=
TE-5 NWW5O4ZvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWnsfnY1UUN3ME2wMlE1Ojd6IN88US=> NVTzb3J1W0GQR1XS
KINGS-1 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXHJR|UxRTBwMUS3PUDPxE1? Ml7NV2FPT0WU
NCI-H69 NEnDWpZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUS1VZJLUUN3ME2wMlE2OTRzIN88US=> NXnzeZo6W0GQR1XS
CAS-1 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlzkTWM2OD1yLkG1OFgzKM7:TR?= MnLCV2FPT0WU
D-263MG NFHFO45Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVPVV4pFUUN3ME2wMlE3ODB4IN88US=> MWfTRW5ITVJ?
A253 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NW\IWVNKUUN3ME2wMlE3OTJ6IN88US=> NGTE[lJUSU6JRWK=
PF-382 NGTINZZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4DUdWlEPTB;MD6xOlcxPiEQvF2= NVnDU3ZNW0GQR1XS
CESS NV7tPGUxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWDJR|UxRTBwMUewOkDPxE1? NY\1eFNlW0GQR1XS
MZ2-MEL M3fw[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIDTSHBKSzVyPUCuNVc2PTlizszN MX3TRW5ITVJ?
HEL MmrSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWPJR|UxRTBwMUi1NVch|ryP MoP6V2FPT0WU
D-392MG NUP5ZldPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmLwTWM2OD1yLkG5NVE2KM7:TR?= M3nibXNCVkeHUh?=
SK-LMS-1 NFr3V2NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1HkV2lEPTB;MD6xPVMxOyEQvF2= MYjTRW5ITVJ?
GI-ME-N M4r0cWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUi2WlhSUUN3ME2wMlE6OzB4IN88US=> NH36dXhUSU6JRWK=
LB831-BLC MlKzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHy5TYpKSzVyPUCuNVk{PCEQvF2= NELpb5JUSU6JRWK=
DU-4475 NVLzT2YxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3jRVWlEPTB;MD6xPVY2QCEQvF2= MmrhV2FPT0WU
IST-SL1 NWf4WoJ{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3f5bGlEPTB;MD6yNFA6PCEQvF2= Ml:4V2FPT0WU
GAK MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1WzXGlEPTB;MD6yNFU{PCEQvF2= NHzSZ5VUSU6JRWK=
EW-1 NH21epVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFr6ZYNKSzVyPUCuNlExPDdizszN NXi5PJVVW0GQR1XS
LAMA-84 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4DiVmlEPTB;MD6yNVg2OSEQvF2= NEG4eZRUSU6JRWK=
SK-UT-1 MkD3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkX0TWM2OD1yLkKyNFQ6KM7:TR?= M17ObXNCVkeHUh?=
VA-ES-BJ MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYroeXc4UUN3ME2wMlIzOjV5IN88US=> NIHQd2VUSU6JRWK=
ACN Mnn0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUHJR|UxRTBwMkK2N|gh|ryP MULTRW5ITVJ?
SK-PN-DW NEPzc3FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHjSVXRKSzVyPUCuNlMyQSEQvF2= NFvVPINUSU6JRWK=
HD-MY-Z NGjJdm5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIXwNnZKSzVyPUCuNlM{ODNizszN NGmweHRUSU6JRWK=
LB373-MEL-D NFTh[ZBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYfJR|UxRTBwMkSxPVgh|ryP NFjvdWRUSU6JRWK=
COLO-829 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlfZTWM2OD1yLkK0NlU4KM7:TR?= M{m3PXNCVkeHUh?=
ES8 MmnwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NITaUnJKSzVyPUCuNlQ4PjNizszN NXjFSZVtW0GQR1XS
RXF393 NYKwWGFqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1rHXWlEPTB;MD6yOVAyPSEQvF2= NH\iOm5USU6JRWK=
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Calu-6 MoPsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVSzTmlmUUN3ME21MlYzOiEQvF2= NFTodVVUSU6JRWK=
ALL-PO MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIPNNGxKSzVyPUWuOlM4QTVizszN M2W3eHNCVkeHUh?=
ARH-77 M3X2OWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHzDd|hKSzVyPUWuOlcxQDdizszN Mkj6V2FPT0WU
A4-Fuk NX:yOFg5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXPFTHZwUUN3ME22MlA5OTF6IN88US=> M{fLNXNCVkeHUh?=
NCI-H1581 NV3vWWZvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmXHTWM2OD14LkKzPFQ6KM7:TR?= Ml;aV2FPT0WU
HUTU-80 MoTRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmTGTWM2OD14LkO2PFk4KM7:TR?= M1T0S3NCVkeHUh?=
TGW M4WyPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWDJR|UxRTZwNEW1PVMh|ryP NVXRSJZWW0GQR1XS
SK-N-FI NF20V4NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUXwTVVNUUN3ME22MlQ2QDV2IN88US=> Mkj3V2FPT0WU
U-266 M2DOTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3y4N2lEPTB;Nj61NVkzPiEQvF2= MV;TRW5ITVJ?
EM-2 NIX3d3NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmTjTWM2OD14Lk[3NFk6KM7:TR?= M2j3SHNCVkeHUh?=
NMC-G1 NH6xR2xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4PjZWlEPTB;Nj63NVg6PSEQvF2= NUDsVW02W0GQR1XS
KASUMI-1 M2rMbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlTvTWM2OD14Lki0O|g4KM7:TR?= NGr0NmVUSU6JRWK=
NALM-6 Ml3US5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2Dv[GlEPTB;Nj64Olc2PCEQvF2= M4rBXXNCVkeHUh?=
OCI-AML2 MoLsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYXJR|UxRTdwMECzNlYh|ryP Mk\oV2FPT0WU
SHP-77 M2rVXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4XDdWlEPTB;Nz6yNlQ{KM7:TR?= MYrTRW5ITVJ?
NOMO-1 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHXRS3hKSzVyPUeuNlQzPTRizszN MnnVV2FPT0WU
SK-N-DZ MkjIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{LSb2lEPTB;Nz63NVA5QSEQvF2= M2XVdXNCVkeHUh?=
LB1047-RCC MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1zESmlEPTB;Nz63NlI{OSEQvF2= NV3aNm1FW0GQR1XS
MZ1-PC NXv5XIR7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{HKXGlEPTB;Nz64OlU2QCEQvF2= M2HnVHNCVkeHUh?=
NB10 NGXBeFJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVvJR|UxRTdwOUm1OVYh|ryP NF33fIlUSU6JRWK=
RL95-2 Mnz3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGr5cnFKSzVyPUiuNVA5PDJizszN NIXnb2lUSU6JRWK=
OMC-1 MlGyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVvJR|UxRThwNUKxPVEh|ryP M2naUnNCVkeHUh?=
D-283MED MnvZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVLnfnhZUUN3ME24MlkyPzF7IN88US=> NUTMR2ZwW0GQR1XS
MC116 NYrtXZYyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3zFPWlEPTB;OD65O|k1KM7:TR?= NEDBN2dUSU6JRWK=
SJSA-1 NUWyeo5RT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkO5TWM2OD17LkC5NFkyKM7:TR?= NYDLVYtEW0GQR1XS
JiyoyeP-2003 M1;MRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHi5e2dKSzVyPUmuNlk{OTdizszN M{fofnNCVkeHUh?=
IST-MEL1 MmPHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEfQVpdKSzVyPUmuO|QyQThizszN NFLOfJZUSU6JRWK=
CTV-1 NXrIVo8xT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYLJR|UxRTFyLkC5O|kh|ryP M2DhOHNCVkeHUh?=
NH-12 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmPBTWM2OD1zMD6yOFM{KM7:TR?= NHT2enZUSU6JRWK=
CA46 M3Hvemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUDJR|UxRTFyLkO2NUDPxE1? M2HLdXNCVkeHUh?=
NCI-SNU-1 MlLhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{jz[WlEPTB;MUCuOFk3QSEQvF2= MlPFV2FPT0WU
SCLC-21H NW\4WlN2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFTSVJhKSzVyPUGwMlY2QTZizszN M2C0bHNCVkeHUh?=
EC-GI-10 M2HL[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXvReHF5UUN3ME2xNE44ODNzIN88US=> NEHOfY9USU6JRWK=
SR MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUDI[XZTUUN3ME2xNU4xOTh3IN88US=> MmX1V2FPT0WU
NCI-H1648 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3fPOmlEPTB;MUGuNFk3PSEQvF2= Mn23V2FPT0WU
TGBC1TKB Mlj0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXK4PFFZUUN3ME2xNU41OTB{IN88US=> MmfOV2FPT0WU
EW-11 NFLaRYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{f0cGlEPTB;MUGuOVE5PiEQvF2= Mn\TV2FPT0WU
SK-MM-2 NH:wRm5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnzVTWM2OD1zMT64NFYyKM7:TR?= NILMOIdUSU6JRWK=
NCI-H524 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MW\JR|UxRTFzLkm4NlIh|ryP M1HxUXNCVkeHUh?=
NOS-1 M124eGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4fp[GlEPTB;MUKuNFM1PSEQvF2= NUSzRXBwW0GQR1XS
AM-38 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYT1SGdTUUN3ME2xNk42PjN|IN88US=> NXvjPZRUW0GQR1XS
A498 NYi1T2h6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3:yW2lEPTB;MUKuO|A3QSEQvF2= M3GydHNCVkeHUh?=
KARPAS-422 NFXh[JhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoniTWM2OD1zMj63OFk3KM7:TR?= NXfoVZR4W0GQR1XS
LU-139 NYHXSoxRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M32zN2lEPTB;MUKuPVAzPiEQvF2= MXTTRW5ITVJ?
COR-L88 NITCcmpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYTJR|UxRTF{LkmzPFIh|ryP NXrRT2hsW0GQR1XS
K5 NXPXdXhQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{GxfWlEPTB;MUKuPVQ3OiEQvF2= NGHvWI9USU6JRWK=
NB13 NGDMe3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXXF[oh6UUN3ME2xNk46Pzh|IN88US=> MlvvV2FPT0WU
MRK-nu-1 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVnGdGRGUUN3ME2xN{4yQTVizszN NU\3OHV{W0GQR1XS
MHH-NB-11 M2Ll[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3nL[GlEPTB;MUOuNlkxOiEQvF2= M1rLbXNCVkeHUh?=
KU812 NVPI[G5JT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mlf1TWM2OD1zMz62NVI1KM7:TR?= M{LWNHNCVkeHUh?=
TE-12 NXzv[mVQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWTTOnR2UUN3ME2xN{43QTl2IN88US=> NH7jdXNUSU6JRWK=
NCI-N87 NX73SIozT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoHITWM2OD1zMz63N|Yh|ryP NFvLNlBUSU6JRWK=
EB2 NWqzOlRJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWrJR|UxRTF|Lki1NFUh|ryP MUPTRW5ITVJ?
DB MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHvXTYdKSzVyPUGzMlk5QDVizszN M{G1PHNCVkeHUh?=
697 MmXvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NU\zNVRrUUN3ME2xOE41PTFzIN88US=> MVvTRW5ITVJ?
MSTO-211H NHjU[FlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MljjTWM2OD1zND63OFQ5KM7:TR?= M4DRZ3NCVkeHUh?=
JVM-2 MkPqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M37E[WlEPTB;MUSuO|c{PSEQvF2= MWXTRW5ITVJ?
COLO-824 NUDRVHFLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoXpTWM2OD1zND63PVk1KM7:TR?= NVjjUXhnW0GQR1XS
BC-3 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEDs[WxKSzVyPUG1MlM6QCEQvF2= MXvTRW5ITVJ?
BOKU NXzpZ2R1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXrDc2twUUN3ME2xOU46PjB6IN88US=> NGLSO21USU6JRWK=
GOTO NHfIW3FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{TEOGlEPTB;MU[uPVYyPyEQvF2= MnnIV2FPT0WU
HCC2157 NV:0dJU5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NU\IT3hVUUN3ME2xO{41PzV3IN88US=> NVHJN|hyW0GQR1XS
LS-1034 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1\JVGlEPTB;MUeuOlgyQCEQvF2= NIHRR3lUSU6JRWK=
CAL-148 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlzvTWM2OD1zNz65O|k3KM7:TR?= NGrLc3lUSU6JRWK=
MOLT-4 M3XSb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVnJR|UxRTF6LkiyNFgh|ryP MYjTRW5ITVJ?
Daudi M33HO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWPYUnNIUUN3ME2xPE46OjNzIN88US=> MmPLV2FPT0WU
J-RT3-T3-5 MmHNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWT3c3RQUUN3ME2xPU41ODZ4IN88US=> MXnTRW5ITVJ?
KMOE-2 NWHpeGNrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHq1VYJKSzVyPUG5MlY1QDJizszN M1nmfnNCVkeHUh?=
HL-60 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnroTWM2OD1{MD6xPVY2KM7:TR?= NWjwb5VSW0GQR1XS
P31-FUJ MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVzYUHA2UUN3ME2yNE41PzV|IN88US=> NF[0TppUSU6JRWK=
IM-9 M17LVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX3OSHBEUUN3ME2yNE43OjJ2IN88US=> MlzGV2FPT0WU
HDLM-2 NUTFVZhET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXK3OmlIUUN3ME2yNE45QDJzIN88US=> NV\pVnBnW0GQR1XS
NCI-H1304 MmPnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{\VNGlEPTB;MkGuNFMyOSEQvF2= NIDIWZNUSU6JRWK=
NCI-H345 MkTZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWDJR|UxRTJzLkC2OFMh|ryP MoTlV2FPT0WU
RPMI-6666 M{PvZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUC1UGM{UUN3ME2yNU4{PjB{IN88US=> NXvxeohmW0GQR1XS
GR-ST MnjGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX7WSlhDUUN3ME2yNU41OTdizszN NYHqV2p6W0GQR1XS
CHP-126 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGrHXXRKSzVyPUKxMlYyQDFizszN NI\JW3BUSU6JRWK=
EHEB M2XpWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkXTTWM2OD1{MT62O|MyKM7:TR?= MYDTRW5ITVJ?
CPC-N M{L4N2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmjiTWM2OD1{ND6wNlAyKM7:TR?= NH3iOWVUSU6JRWK=
NB1 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmXyTWM2OD1{ND62PVM6KM7:TR?= M1rpfXNCVkeHUh?=
LS-123 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2mw[2lEPTB;MkSuPVAyOiEQvF2= NXe0UmR2W0GQR1XS
ST486 M1juTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVzJR|UxRTJ3LkGxNVMh|ryP M3;E[nNCVkeHUh?=
NCI-H1963 NGDESXJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYPJR|UxRTJ4LkC2NlMh|ryP M4rJR3NCVkeHUh?=
U-87-MG MnjlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2D3R2lEPTB;Mk[uO|Y1PCEQvF2= MYHTRW5ITVJ?
COR-L279 MknQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXi0cWJsUUN3ME2yOk44QTJ{IN88US=> NFrUN2ZUSU6JRWK=
LU-165 NV\zNYxrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2nqPGlEPTB;MkiuNFg3OSEQvF2= MmDzV2FPT0WU
COLO-800 NVTJXWF3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{[zSGlEPTB;MkiuNlk2PiEQvF2= NWnubWNEW0GQR1XS
ETK-1 NHLmb49Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWrJR|UxRTJ6LkO0OFYh|ryP NXrKUGw6W0GQR1XS
LNCaP-Clone-FGC NGTEb4tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmTiTWM2OD1{OT65NlgyKM7:TR?= MVrTRW5ITVJ?
SIG-M5 NGrHUGlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFPsRYdKSzVyPUOwMlc6PTJizszN M13tfXNCVkeHUh?=
NB6 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWTJR|UxRTNyLkm1NFMh|ryP MnfTV2FPT0WU
NCI-H2107 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlLZTWM2OD1|MT6yN|M5KM7:TR?= NITQU45USU6JRWK=
SNU-C1 M1nMRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MljHTWM2OD1|MT6zNVI3KM7:TR?= M{W3c3NCVkeHUh?=
JAR MlPhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVzJR|UxRTNzLkO3PVkh|ryP MkjzV2FPT0WU
L-363 NH\5RVZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWHIVlZKUUN3ME2zNk4zOTR2IN88US=> NIrRUlhUSU6JRWK=
EW-24 Mnz2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1H2PGlEPTB;M{KuN|Q4PCEQvF2= MX;TRW5ITVJ?
NB69 MlSxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVLBVGZWUUN3ME2zN{43PDV2IN88US=> NHvqUGFUSU6JRWK=
EW-13 NWTROWJiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnTPTWM2OD1|Mz64PVY6KM7:TR?= NEPpUFlUSU6JRWK=
Ramos-2G6-4C10 M13SRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFjROolKSzVyPUO0MlI6QTNizszN NXX4ZW1HW0GQR1XS
TE-11 NGK2cmZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWPJR|UxRTN2LkS4NlIh|ryP NHHEUmFUSU6JRWK=
L-428 M4PafWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHHkT3dKSzVyPUO0Mlc2OzZizszN MXjTRW5ITVJ?
KP-N-YN MmLZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX3JR|UxRTN2LkmwOVch|ryP MoLJV2FPT0WU
CGTH-W-1 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2jld2lEPTB;M{[uPVA1QCEQvF2= NWjL[Xc6W0GQR1XS
K-562 NF\xOVVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{DpZWlEPTB;M{euNFg3PCEQvF2= M4fQS3NCVkeHUh?=
NCI-H1299 NW\5SYdmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGfvS4xKSzVyPUO4MlE3ODNizszN M3zSb3NCVkeHUh?=
RCC10RGB NFvzS5dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEHqWZJKSzVyPUO4MlIxQDNizszN NVywO5J[W0GQR1XS
NCI-SNU-16 NFHzZZZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWTvVGdQUUN3ME2zPE42PjV|IN88US=> NVu3T4ExW0GQR1XS
LC-2-ad MmHqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4rtNmlEPTB;M{muOFM{PyEQvF2= Mn24V2FPT0WU
MHH-PREB-1 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4PGVGlEPTB;M{muO|M5QCEQvF2= M2PhUHNCVkeHUh?=
NCI-H64 MoTvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHLSZ4ZKSzVyPUSwMlA6PDhizszN NHO5WWhUSU6JRWK=
LB996-RCC MoG4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXTaN2F1UUN3ME20NE45PzF4IN88US=> NYH5W4ZJW0GQR1XS
DEL NWC4b|VIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUjJR|UxRTRzLkS0NFUh|ryP MUjTRW5ITVJ?
MLMA NYGzbJAyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWPt[Y5FUUN3ME20NU44PTl3IN88US=> MkfBV2FPT0WU
SBC-1 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mo\uTWM2OD12Mj6xPFA3KM7:TR?= M1X2NXNCVkeHUh?=
MPP-89 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmiyTWM2OD12Mj61PFc2KM7:TR?= MVjTRW5ITVJ?
MV-4-11 NW\EfFQxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2rmPGlEPTB;NEKuPVA3PyEQvF2= NFH0UJJUSU6JRWK=
EoL-1- Mm\HS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUnwW3FqUUN3ME20OE4yPTZ6IN88US=> MXvTRW5ITVJ?
CW-2 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1u3NWlEPTB;NESuPFM1OiEQvF2= MmHBV2FPT0WU
HT M1fwd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1H3dGlEPTB;NEWuO|AxPyEQvF2= MXLTRW5ITVJ?
SW954 NUfKUHFuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXjlXms{UUN3ME20O{41OTJ6IN88US=> MoTPV2FPT0WU
A3-KAW NX3jb|NMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIHl[29KSzVyPUS5MlgxPjFizszN NYDPeoVrW0GQR1XS
TC-YIK NY\Qdnh1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4TpPGlEPTB;NUCuNFM3OyEQvF2= NHv6XJFUSU6JRWK=
SW962 NYjD[mRXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3vldGlEPTB;NUSuPFM2PyEQvF2= MlPFV2FPT0WU
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NCI-H1694 M3nLSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnG4TWM2OD15MT6wN|Ih|ryP NUi4bIZkW0GQR1XS
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NCI-H2171 M1uxPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGTmRmRKSzVyPUe5MlQyOzZizszN MV7TRW5ITVJ?
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LU-134-A MnTyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEHkVoFKSzVyPUizMlE{PTFizszN MorsV2FPT0WU
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NCI-H889 NVrjdHluT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnXPTWM2OD1zMESuNlQzKM7:TR?= MV\TRW5ITVJ?
EW-22 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUfJR|UxRTFyNj6xPUDPxE1? MYTTRW5ITVJ?
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BE-13 NHvWVmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV:yR2N[UUN3ME2xN|QvODR2IN88US=> M333ZXNCVkeHUh?=
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... Click to View More Cell Line Experimental Data

In vivo Administration of MG-132 effectively rescues the expression levels and plasma membrane localization of dystrophin, β-dystroglycan, α-bdystroglycan, and α-sarcoglycan in skeletal muscle fibers from mdx mice, reduces muscle membrane damage, and ameliorates the histopathological signs of muscular dystrophy. [7] MG-132 treatment significantly reduces immobilization-induced skeletal muscle atrophy in mice, by downregulating the muscle-specific ubiquitin ligases atrogin-1/MAFbx and MuRF-1 mRNA. [8]

Protocol

Kinase Assay:

[1]

+ Expand

Measurement of inhibitory activities of MG-132 against 20S proteasome:

The reaction mixture for the 20S proteasome inhibitory assay contains 0.1 M Tris-acetate, pH 7.0, 20S proteasome, MG-132, and 25 μM substrate dissolved in dimethyl sulfoxide in a final volume of 1 mL. After incuba tion at 37 °C for 15 minutes, the reaction is stopped by the addition of 0.1 mL of 10% SDS and 0.9 mL of 0.1M Tris acetate, pH 9.0. The fluorescence of the reaction products is measured. To determine the IC50 against 20S proteasome, various concentrations of MG-132 are included in the assay mixture.
Cell Research:

[3]

+ Expand
  • Cell lines: KIM-2, HC11, and ES
  • Concentrations: Dissolved in DMSO, final concentrations ~25 μM
  • Incubation Time: 24 and 48 hours
  • Method:

    Cells are exposed to various concentrations of MG-132 for 24, and 48 hours. Supernatant and monolayer cells are harvested by centrifugation and fixed in 70% ethanol in PBS for staining with acridine orange. Equal volumes of cells and acridine orange (5 mg/mL in PBS) are mixed on a microscope slide and examined by fluorescence microscopy. For annexin V analysis, cells are harvested by centrifugation and stained with annexin V and propidium iodide. For cell cycle analysis, cells are rehydrated in PBS at room temperature for 10 minutes, followed by staining with propidium iodide (5 mg/mL). All samples are analyzed using a Coulter Epics XL flow cytometer.


    (Only for Reference)
Animal Research:

[7]

+ Expand
  • Animal Models: Male mdx (C57BL/10ScSn DMD mdx) mice
  • Formulation: Dissolved in DMSO, and diluted in PBS
  • Dosages: ~10 μg/kg/day
  • Administration: Injection
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 90 mg/mL (189.22 mM)
Ethanol 25 mg/mL (52.56 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+20% propylene glycol+ddH2O
For best results, use promptly after mixing.
2mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 475.62
Formula

C26H41N3O5

CAS No. 133407-82-6
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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Definitions of molecular mass, molecular weight, molar mass and molar weight:

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Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    I want to know whether it contains EDTA or other chelating agent in this product(S2619)?

  • Answer:

    Our S2619 MG-132 doesn't contain EDTA or other chelating agent.

Proteasome Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID