Torin 1

Catalog No.S2827

Torin 1 Chemical Structure

Molecular Weight(MW): 607.62

Torin 1 is a potent inhibitor of mTORC1/2 with IC50 of 2 nM/10 nM in cell-free assays; exhibits 1000-fold selectivity for mTOR than PI3K.

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  • Flag-tagged SENP3 was immunoprecipitated from HeLa cells and used for in vitro phosphorylation assay with [32P]ATP with either the mock-treated or Torin1-treated mTOR/Raptor/MLST8 complex. (Upper) The samples then were subjected to SDS-PAGE, followed by autoradiography. mTOR autophosphorylation is indicated by an asterisk. (Lower) Immunopurified SENP3 was monitored by Western blotting with anti-Flag antibody.

    Mol Cell Biol 2014 34(24), 4474-84. Torin 1 purchased from Selleck.

    Huh-7.5 cells were treated with increasing concentration of Torin 1, a known autophagy inducer, and the autophagy response was assessed by measuring LC3II and p62 levels.

    Am J Pathol 2014 184(1), 214-29. Torin 1 purchased from Selleck.

  • Representative Western blots showing LC3 in PP242 (PP) or Torin1 (Tor) treated HepG2 cells. HepG2 cells were treated with 10 uM PP242 or 0.1 uM Torin1 for 24 h.

    Biochem Biophys Res Commun 2014 10.1016/j.bbrc.2014.05.047. Torin 1 purchased from Selleck.

    Bone marrow derived macrophages were pre-treated with 10nM Torin 1 for 1h prior to LPS treatment (100 ng/ml). TNF-a production was analyzed 24h later.

    Lim Hsiu Kim, Lina from National University of Singapore. Torin 1 purchased from Selleck.

Purity & Quality Control

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Notes:

2. For more details, such as half maximal inhibitory concentrations (IC50s) and working concentrations of each inhibitor, please click on the link of the inhibitor of interest.
3. "+" indicates inhibitory effect. Increased inhibition is marked by a higher "+" designation.
4. Orange "√" refers to compounds which do inhibitory effects on the related isoform, but without specific value.

Biological Activity

Description Torin 1 is a potent inhibitor of mTORC1/2 with IC50 of 2 nM/10 nM in cell-free assays; exhibits 1000-fold selectivity for mTOR than PI3K.
Targets
mTORC1 [1]
(Cell-free assay)
mTOR [2]
(Cell-free assay)
DNA-PK [2]
(Cell-free assay)
mTORC2 [1]
(Cell-free assay)
p110γ [2]
(Cell-free assay)
2 nM 4.32 nM 6.34 nM 10 nM 171 nM
In vitro

Torin1 inhibits phosphorylation of mTORC1 and mTORC2 substrates in cells at concentrations of 2 and 10 nM, respectively. Moreover, Torin1 exhibits 1000-fold selectivity for mTOR over PI3K (EC50 = 1800 nM) and exhibits 100-fold binding selectivity relative to 450 other protein kinases. [1] [2] Torin1 causes cell cycle arrest through a rapamycin-resistant mechanism that is also independent of mTORC2. Torin1 disrupts mTORC1-dependent phenotypes more completely than rapamycin. Rapamycin-resistant functions of mTORC1 are required for cap-dependent translation. [1] In a recent study, it is reported Torin1 increases neurotensin secretion and gene expression through activation of the MEK/ERK/c-Jun pathway in the human endocrine cell line BON. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
ETK-1 NUnhd3ZTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{\kZWlEPTB;MD6wNFA{ODZizszN NXf2W4t{W0GQR1XS
OCUB-M Mm\MS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUTJR|UxRTBwMECxPVQh|ryP NGDsU3NUSU6JRWK=
DOHH-2 M1LD[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV\SV2dKUUN3ME2wMlAxOjJ3IN88US=> NVznO4o3W0GQR1XS
NOS-1 NW\BbZc5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4jtVmlEPTB;MD6wNFI1OiEQvF2= MX7TRW5ITVJ?
L-363 NIP4Z2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVHPZoczUUN3ME2wMlAxOjh2IN88US=> NFrhTWlUSU6JRWK=
RL95-2 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX25ZYREUUN3ME2wMlAxOzJizszN MXjTRW5ITVJ?
BL-70 M3n2Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWTJR|UxRTBwMECzN|ch|ryP MkfyV2FPT0WU
TGBC1TKB M1LncWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFfiblNKSzVyPUCuNFA{PzlizszN MXnTRW5ITVJ?
697 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MknNTWM2OD1yLkCwOlM1KM7:TR?= NHjySHJUSU6JRWK=
RPMI-8226 MkPiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1zLd2lEPTB;MD6wNFY2OSEQvF2= MnzwV2FPT0WU
BL-41 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV3JR|UxRTBwMEC2PVYh|ryP M3TaSnNCVkeHUh?=
MRK-nu-1 NYLRZZhUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUfJR|UxRTBwMEC3NFch|ryP M4LLUHNCVkeHUh?=
EW-1 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1faOGlEPTB;MD6wNFc1QSEQvF2= M{DSe3NCVkeHUh?=
EVSA-T MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWXJR|UxRTBwMEC4PVIh|ryP MXfTRW5ITVJ?
HUTU-80 NGLET5lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{mwN2lEPTB;MD6wNFkzQSEQvF2= NEjlbWRUSU6JRWK=
VA-ES-BJ NUjBTXRsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{XCWmlEPTB;MD6wNFk5PCEQvF2= NUXkPYdKW0GQR1XS
GCIY MofOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYW4XVNuUUN3ME2wMlAyODB5IN88US=> MUjTRW5ITVJ?
ES6 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV\IU205UUN3ME2wMlAyODh|IN88US=> Mm\WV2FPT0WU
NALM-6 MmPDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXLM[48yUUN3ME2wMlAyOTB{IN88US=> Ml3DV2FPT0WU
LB996-RCC MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MljVTWM2OD1yLkCxNVM4KM7:TR?= NHPvNmpUSU6JRWK=
EW-16 NFf6VnlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVjFOXJyUUN3ME2wMlAyOTl{IN88US=> M1fwOnNCVkeHUh?=
KALS-1 NVzk[JhvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYjJR|UxRTBwMEGxPVgh|ryP NGrYcFRUSU6JRWK=
OPM-2 NXv4U3g4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWntc5F4UUN3ME2wMlAyOjlizszN MVjTRW5ITVJ?
KU812 M4PWZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHPZbXVKSzVyPUCuNFEzQTZizszN M173d3NCVkeHUh?=
A4-Fuk M2[1U2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFvzPWVKSzVyPUCuNFE{OTlizszN NH;nZ2xUSU6JRWK=
KGN M4LTSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYXj[5pQUUN3ME2wMlAyOzV7IN88US=> NIHKc3VUSU6JRWK=
KS-1 NWjtZo9GT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFXRW2FKSzVyPUCuNFE{QTJizszN NFjjNWJUSU6JRWK=
no-11 MnvKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVnEZ3FOUUN3ME2wMlAyOzl3IN88US=> M1rUZ3NCVkeHUh?=
PSN1 NV3FXoxVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVPSTIZKUUN3ME2wMlAyPDB7IN88US=> MXvTRW5ITVJ?
SK-LMS-1 NVPm[m9OT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWjQRWlYUUN3ME2wMlAyPDF|IN88US=> NWS0bYRzW0GQR1XS
LS-411N MoG0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWDiW2RjUUN3ME2wMlAyPDN7IN88US=> Ml7KV2FPT0WU
HT-144 NEewTo5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXLJR|UxRTBwMEG0OVEh|ryP M4DqSnNCVkeHUh?=
ACN MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVWxdZNrUUN3ME2wMlAyPSEQvF2= MoL5V2FPT0WU
RKO NEj1OWhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MY\JR|UxRTBwMEG1N|Mh|ryP NXm5XphmW0GQR1XS
CMK NGjicnZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVfSfI06UUN3ME2wMlAyPTR|IN88US=> NXHYWpJLW0GQR1XS
GB-1 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MW\JR|UxRTBwMEG1OVMh|ryP NEnJT25USU6JRWK=
KE-37 MnmxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkXJTWM2OD1yLkCxOVc{KM7:TR?= NV;4ZZlmW0GQR1XS
no-10 M3XNOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXHJR|UxRTBwMEG1PFQh|ryP M135UXNCVkeHUh?=
MOLT-16 M2n0VWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXuwN2ZiUUN3ME2wMlAyPjJ{IN88US=> NXnJclZTW0GQR1XS
HEL M1[5UWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4nvTWlEPTB;MD6wNVY1KM7:TR?= MV\TRW5ITVJ?
NCI-H1355 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnPWTWM2OD1yLkCxOlQ3KM7:TR?= NVm3[VRjW0GQR1XS
IST-MEL1 M2fPS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXPJR|UxRTBwMEG3O|Qh|ryP NX7iWnFuW0GQR1XS
MHH-PREB-1 NV3hWJJVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2fv[mlEPTB;MD6wNVgyQCEQvF2= NFntdllUSU6JRWK=
ES8 M1rBZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{XX[GlEPTB;MD6wNVg1PCEQvF2= NWewclV2W0GQR1XS
LC-2-ad NYDvdmRHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH31UIdKSzVyPUCuNFIyOzdizszN MY\TRW5ITVJ?
LAMA-84 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2PIeGlEPTB;MD6wNlE3PyEQvF2= NW\YeVNmW0GQR1XS
BV-173 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGPVfZVKSzVyPUCuNFIzODNizszN NXzlWVVGW0GQR1XS
SW872 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M37q[mlEPTB;MD6wNlI1PyEQvF2= NFzSOGFUSU6JRWK=
SK-UT-1 Mmq0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUXrNW4{UUN3ME2wMlAzOjZzIN88US=> NE\4cmdUSU6JRWK=
OS-RC-2 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mk\GTWM2OD1yLkCyN|I{KM7:TR?= MonQV2FPT0WU
NCI-H747 NILsOHpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFHjOHZKSzVyPUCuNFI{OyEQvF2= MYjTRW5ITVJ?
MZ2-MEL NHv1XGtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MW\JR|UxRTBwMEKzN|ch|ryP M3KyT3NCVkeHUh?=
TE-11 Mn;vS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH7BSoRKSzVyPUCuNFI{PzJizszN NYDaXGVCW0GQR1XS
KURAMOCHI NWL0Opl5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkPpTWM2OD1yLkCyOFE1KM7:TR?= Mmq1V2FPT0WU
HD-MY-Z MoXwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWe1O3dnUUN3ME2wMlAzPDJ4IN88US=> M4HQd3NCVkeHUh?=
GI-1 M{XDRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWLJR|UxRTBwMEK0Olkh|ryP M3jITnNCVkeHUh?=
LB1047-RCC M4m3R2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmnGTWM2OD1yLkCyOFg5KM7:TR?= M3Xxd3NCVkeHUh?=
TE-12 M1LhOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV7xVIUzUUN3ME2wMlAzPTVizszN MonyV2FPT0WU
MZ7-mel NX;Zc3RMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUjJR|UxRTBwMEK2NFkh|ryP M3XTZnNCVkeHUh?=
MMAC-SF NH7NeXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUDycJVmUUN3ME2wMlAzPjN|IN88US=> M3PKenNCVkeHUh?=
A101D NX;ReZJwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkXlTWM2OD1yLkCyOlUh|ryP M36wZXNCVkeHUh?=
GR-ST MnjpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEK0cnpKSzVyPUCuNFI3PTVizszN NF\telRUSU6JRWK=
MC116 M2fRSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXTJR|UxRTBwMEK2PEDPxE1? NGXsTWpUSU6JRWK=
Ramos-2G6-4C10 M{HuPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV3JR|UxRTBwMEK3OUDPxE1? NVTzVW9IW0GQR1XS
ATN-1 NGrjSYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlzrTWM2OD1yLkCyO|g6KM7:TR?= MUPTRW5ITVJ?
MFH-ino MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV3vbmNrUUN3ME2wMlAzQDF{IN88US=> NXTHdopFW0GQR1XS
DEL NUCxSnR5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHOyOZVKSzVyPUCuNFI5OTVizszN M1uwT3NCVkeHUh?=
OCI-AML2 NV\sWotGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXXG[WttUUN3ME2wMlAzQDdzIN88US=> NVz1[292W0GQR1XS
LXF-289 M{XnU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYPhe3lOUUN3ME2wMlAzQDd{IN88US=> M1TzeXNCVkeHUh?=
IA-LM M2PCV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnTqTWM2OD1yLkCyPVE4KM7:TR?= NELtR5NUSU6JRWK=
EoL-1-cell M4eyTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NI\jZnBKSzVyPUCuNFI6QTZizszN MX3TRW5ITVJ?
OVCAR-4 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV\BcVRVUUN3ME2wMlA{ODl2IN88US=> M{fKRXNCVkeHUh?=
ES1 NHqzZXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1H6R2lEPTB;MD6wN|A6PyEQvF2= NXrQNG4yW0GQR1XS
CESS NF;xcodIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYTKfIRvUUN3ME2wMlA{ODl7IN88US=> MYLTRW5ITVJ?
GAK MnTVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MonMTWM2OD1yLkCzNVg3KM7:TR?= NV\V[WhZW0GQR1XS
NTERA-S-cl-D1 M2HtU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmrJTWM2OD1yLkCzNlI3KM7:TR?= MXXTRW5ITVJ?
TE-10 MkjrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHj3WnBKSzVyPUCuNFM{OTFizszN NXHUVVE6W0GQR1XS
CCRF-CEM NF;xNoFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUjJR|UxRTBwMEOzO|Eh|ryP MYLTRW5ITVJ?
NMC-G1 MkXCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVzDUWZnUUN3ME2wMlA{PDR6IN88US=> NHfXOHNUSU6JRWK=
HOP-62 NXP1PI83T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFfvfZNKSzVyPUCuNFM2PTZizszN NUPM[mdtW0GQR1XS
SH-4 M{nL[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnXYTWM2OD1yLkCzOVgzKM7:TR?= NYfROHA2W0GQR1XS
NCI-H1648 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NELSUmVKSzVyPUCuNFM3OjJizszN NGXUbotUSU6JRWK=
EB-3 M1XLb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NETQTVdKSzVyPUCuNFM3PDNizszN MUjTRW5ITVJ?
BB65-RCC NWP4VVF4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXLJR|UxRTBwMEO2PVEh|ryP MV;TRW5ITVJ?
BE-13 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEHG[4lKSzVyPUCuNFM4PTlizszN MXXTRW5ITVJ?
CP67-MEL NHzOc4FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1HTW2lEPTB;MD6wN|c2QSEQvF2= NYDl[I5kW0GQR1XS
SW954 NWTpeZhsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{jKNWlEPTB;MD6wN|c6KM7:TR?= NGfBWnpUSU6JRWK=
TE-5 M2PZc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXy3dJJyUUN3ME2wMlA{QDV2IN88US=> NX30NYRiW0GQR1XS
HCE-4 MnLJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEf4SpdKSzVyPUCuNFM5PTVizszN NHvQ[5BUSU6JRWK=
LC-1F NG\sfYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX;GdoNvUUN3ME2wMlA{QTh7IN88US=> MUXTRW5ITVJ?
TK10 NVrLdHZwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NULvdVQ5UUN3ME2wMlA1ODF7IN88US=> NVOzdG5YW0GQR1XS
SJSA-1 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYjJR|UxRTBwMESxPFgh|ryP MlqzV2FPT0WU
Daudi M{TDdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHvK[YtKSzVyPUCuNFQzOjVizszN MXzTRW5ITVJ?
SW982 NIX4eFlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmHYTWM2OD1yLkC0NlQ3KM7:TR?= MnrvV2FPT0WU
SF126 Mni0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVXJR|UxRTBwMESzOlEh|ryP NWTVT4xDW0GQR1XS
TE-15 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1HQXmlEPTB;MD6wOFQ{PSEQvF2= Mk[zV2FPT0WU
COLO-684 MnXyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFzhcYxKSzVyPUCuNFQ2PjFizszN NIX3[XhUSU6JRWK=
8-MG-BA NImweoRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmrLTWM2OD1yLkC0OlI6KM7:TR?= MnqyV2FPT0WU
NB13 NWHnN2pQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{e1TmlEPTB;MD6wOFc4PiEQvF2= NE\FZ2pUSU6JRWK=
HCC1599 M{PlUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXLJR|UxRTBwMES4O|kh|ryP M4PvN3NCVkeHUh?=
SK-PN-DW MonaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXjJR|UxRTBwMES5OFkh|ryP NVHo[IY1W0GQR1XS
ALL-PO NV\icYJmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml;kTWM2OD1yLkC0PVU3KM7:TR?= NH\WRZpUSU6JRWK=
NB6 M{fYU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1LkfmlEPTB;MD6wOVI4OSEQvF2= NYfwb20yW0GQR1XS
IST-SL2 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnLrTWM2OD1yLkC1N|YzKM7:TR?= M3ziSnNCVkeHUh?=
BB30-HNC NYH6T4dCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NW\pSlM{UUN3ME2wMlA2PzNizszN NYn4VplHW0GQR1XS
SW962 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4\E[WlEPTB;MD6wOVc3OyEQvF2= NFvPS3VUSU6JRWK=
TGBC24TKB NIe4ZmhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUe5fndOUUN3ME2wMlA2Pzl7IN88US=> NYm4NXlDW0GQR1XS
KINGS-1 M2i3OWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWrSW|JFUUN3ME2wMlA2QDB|IN88US=> MWPTRW5ITVJ?
HC-1 NV;qS2NjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWW4bXd[UUN3ME2wMlA2QDJ5IN88US=> NIX2S4VUSU6JRWK=
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REH MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX7FNINtUUN3ME2xMlcyODd6IN88US=> MmfRV2FPT0WU
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NCI-H345 NWHuXppOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIfWS4tKSzVyPUKuOlE6ODVizszN M36yTXNCVkeHUh?=
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JVM-3 Mo\XS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnzsTWM2OD13LkK1NFE1KM7:TR?= NF7OWYhUSU6JRWK=
LAN-6 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3ftc2lEPTB;NT61NVc{PiEQvF2= NYnKOGNuW0GQR1XS
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U-87-MG MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXvndGlFUUN3ME21Mlg6QTZ2IN88US=> NFnKe3dUSU6JRWK=
SHP-77 MkHlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFuzTnBKSzVyPUWuPVQ6OjhizszN MX;TRW5ITVJ?
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ES7 NXmyeFRmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MULJR|UxRTZwM{m5NlQh|ryP NFvyRplUSU6JRWK=
WSU-NHL Mn;OS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2jlVGlEPTB;Nj61NVYxQSEQvF2= NFrLWoxUSU6JRWK=
CAS-1 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGjEbYlKSzVyPUeuNFk4PDlizszN MXrTRW5ITVJ?
MHH-NB-11 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX6zTZVXUUN3ME24MlI{OTV{IN88US=> NGDabXdUSU6JRWK=
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HDLM-2 NV;vXWJiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHXjcXRKSzVyPUmuPVU6ODJizszN MlnmV2FPT0WU
NCI-H2171 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUXJR|UxRTFyLkW5Nlch|ryP MXnTRW5ITVJ?
LNCaP-Clone-FGC NVjnWGZRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkTyTWM2OD1zMT6zO|E5KM7:TR?= MX7TRW5ITVJ?
NCI-H1395 NYXSXnBPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYXUUoY5UUN3ME2xNk4zPjl7IN88US=> NEiz[FJUSU6JRWK=
NCI-H1436 NVrwU3p[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYLJR|UxRTF|LkOxPVUh|ryP MX;TRW5ITVJ?
RCC10RGB NHTkd41Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIrITHpKSzVyPUGzMlMzOjFizszN NYjIZ3BoW0GQR1XS
SW684 NI\VR3FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVj1UldSUUN3ME2xN{46Pzl5IN88US=> NF\WO3FUSU6JRWK=
NCI-H1650 MmrDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnjsTWM2OD1zNT6wOFQ4KM7:TR?= NFXXcpNUSU6JRWK=
EW-13 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1jyeWlEPTB;MUWuNlAzOSEQvF2= MXLTRW5ITVJ?
EC-GI-10 M2OxVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml3vTWM2OD1zNT64OlQ1KM7:TR?= NXTm[W1tW0GQR1XS
CHP-126 NIDrWItIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2rYXGlEPTB;MUeuNVkyQCEQvF2= NY\PWYlbW0GQR1XS
NCI-H226 NV\POINVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHPRPXVKSzVyPUG4MlA1ODNizszN NYLTSW8zW0GQR1XS
NCI-H1838 NYjUbGZCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV7j[FllUUN3ME2yNE4yQDR6IN88US=> MXLTRW5ITVJ?
EW-11 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXHJR|UxRTJ2LkK3NVgh|ryP M1PDNXNCVkeHUh?=
EB2 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVj1R5JuUUN3ME2zNU42OzF6IN88US=> NIXUNYlUSU6JRWK=
D-502MG MkDJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH3jXWhKSzVyPUSzMlkzOjlizszN NUWwfm1OW0GQR1XS
U-698-M MmewS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHyzemFKSzVyPUS4Mlg3PzFizszN MWDTRW5ITVJ?
ES3 M4LVZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{C2[2lEPTB;NEmuN|g6OyEQvF2= MlexV2FPT0WU

... Click to View More Cell Line Experimental Data

In vivo Torin1 is efficacious at a dose of 20 mg/kg in a U87MG xenograft model and demonstrates good pharmacodynamic inhibition of downstream effectors of mTOR in tumor and peripheral tissues. [2]

Protocol

Kinase Assay
+ Expand

mTORC1 and mTORC2 in Vitro Kinase Assays:

To produce soluble mTORC1, HEK-293T cell lines that stably express N-terminally FLAG-tagged Raptor are generated using vesicular stomatitis virus G-pseudotyped MSCV retrovirus. For mTORC2, similar HeLa cells that stably express N-terminally FLAG-tagged Protor-1 are generated. Both complexes are purified by lysing cells in 50 mm HEPES, pH 7.4, 10 mm sodium pyrophosphate, 10 mm sodium β-glycerophosphate, 100 mm NaCl, 2 mm EDTA, 0.3% CHAPS. Cells are lysed at 4 °C for 30 min, and the insoluble fraction is removed by microcentrifugation at 13,000 rpm for 10 min. Supernatants are incubated with FLAG-M2 monoclonal antibody-agarose for 1 h and then washed three times with lysis buffer and once with lysis buffer containing a final concentration of 0.5 M NaCl. Purified mTORC1 is eluted with 100 μg/mL 3×FLAG peptide in 50 mm HEPES, pH 7.4, 100 mm NaCl. Eluate can be aliquoted and stored at -80 °C. Substrates S6K1 and Akt1 are purified. Kinase assays are performed for 20 min at 30 °C in a final volume of 20 μL consisting of the kinase buffer (25 mm HEPES, pH 7.4, 50 mm KCl, 10 mm MgCl2, 500 μm ATP) and 150 ng of inactive S6K1 or Akt1 as substrates. Reactions are stopped by the addition of 80 μL of sample buffer and boiled for 5 min. Samples are subsequently analyzed by SDS-PAGE and immunoblotting.
Cell Research
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  • Cell lines: MEFs
  • Concentrations: ~250 nM
  • Incubation Time: 4 days
  • Method: Cell viability is assessed with the CellTiter-Glo Luminescent Cell Viability Assay. On Day 0, 96-well plates are seeded with 500 cells per well and grown overnight. On Day 1, cells are treated with the appropriate compounds and subsequently analyzed on Days 3-5. For analysis, plates are incubated for 60 min at room temperature; 50 μL of CellTiter-Glo reagent is added to each well, and plates are mixed on an orbital shaker for 12 min. Luminescence is quantified on a standard plate luminometer.
    (Only for Reference)
Animal Research
+ Expand
  • Animal Models: U87MG xenograft model
  • Formulation: Torin1 powder is first dissolved at 25 mg/mL in 100% N-methyl-2-pyrrolidone and subsequently diluted 1:4 with sterile 50% PEG400 to a final concentration of 5 mg/mL
  • Dosages: 20 mg/kg
  • Administration: Administered via i.p. once daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 2 mg/mL (3.29 mM)
Water <1 mg/mL
Ethanol <1 mg/mL
In vivo 30% PEG 400+0.5% Tween80+5% Propylene glycol 30mg/mL

* 1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 607.62
Formula

C35H28F3N5O2

CAS No. 1222998-36-8
Storage powder
in solvent
Synonyms N/A

Bio Calculators

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

  • Question 1:

    How to re-constitute the inhibitor for i.p. administration?

  • Answer:

    S2827 Torin 1 can be dissolved in 5% DMSO/30% PEG 300/ddH2O at 0.25 mg/ml clearly, and in 10% DMSO/corn oil at 0.5 mg/ml, it is also a clear solution.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID