AZD8055

Catalog No.S1555

AZD8055 Chemical Structure

Molecular Weight(MW): 465.54

AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. Phase 1.

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In DMSO USD 156 In stock
USD 120 In stock
USD 370 In stock

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Cited by 37 Publications

7 Customer Reviews

  • PC3-LN4 cells were treated with GSK690693 (5 uM) alone, or in combination with PP242 (2 uM) or AZD8055 (1 uM) for 24 h, and immunoblotting performed.

    Cancer Res 2013 73(11), 3402-11. AZD8055 purchased from Selleck.

    HUVEC were treated with either RAD001 or AZD8055 before PA treatment. p53, GPX1, p53 acetylation, and subcellular PTEN were analyzed with corresponding antibodies. The blot is a representative of the results of three experiments. Values (mean盨D) are expressed as fold over untreated control. *p<0.05 versus their corresponding control groups in the one-way ANOVA.

    Antioxid Redox Signal 2014 20(9), 1382-95. AZD8055 purchased from Selleck.

  • (A) HCT116 or MDA-MB-468 cells were treated with 500 nM AZD8055 for the indicated times. Cell lysates were immunoblotted with the indicated antibodies. (B) HCT116 or MDA-MB-468 cells were treated with 50 nM rapamycin. 500 nM AZD8055, 1 uM MK2206 or dimethyl sulfoxide (DMSO) as control for 12 h. Cell lysates were immunoblotted with the indicated antibodies.

    Oncotarget 2014 5(15), 6015-27. AZD8055 purchased from Selleck.

    CSCs+24/44/ESA at 5,000 cells per well with stem cell culture media in triplicate in an ultra-low adhesion plate were treated with 5 umol/L BMS-777607, 1 umol/L AZD8055 alone, or in their different combinations. Cells were cultured for 72 hours. Cell viability was determined by the MTS assay. Percentages of polyploid cells were determined by counting 300 cells from two different regions. Results shown here were from one of two experiments with similar results.

    Mol Cancer Ther 2014 13(1), 37-48. AZD8055 purchased from Selleck.

  • mTOR kinase inhibitor AZD8055 activates PI3K accompanied with induction of expression of EGFR, HER2, HER3 and IRS1. Serum-deprived CHO-EGFP-AKT cells were incubated with 50 nM AZD8055 for 24 hr. The EGFP signal was detected using confocal microscopy. The white arrows indicate EGFP-AKT located on cellular membrane.

    Int J Cancer 2014 135(10), 2462-74. AZD8055 purchased from Selleck.

    mTOR, P70S6K1, 4E-BP1 and PCNA activation in the basilar artery of SAH. Top, Western blotting bands of antibodies, bottom, density of the protein band from each group. A, mTOR activation in the basilar artery demonstrated an elevated expression of mTOR on day 7 following SAH in SAH and SAH+DMSO animals. The mTOR inhibitors, Rapamycin and AZD8055 reduced the enhanced expression of mTOR (pb0.05, ANOVA). B. P70S6K1 activation. C. 4E-BP1 activation. D. PCNA activation. Rapamycin and AZD8055 significantly decreased the expression of P70S6K1, 4E-BP1 and PCNA compared with the SAH and SAH+DMSO (pb0.05, respectively; ANOVA). There was no significant difference between the two inhibitors in mTOR, P70S6K1, 4E-BP1 and PCNA expression.

    Exp Neurol 2012 233, 799-806. AZD8055 purchased from Selleck.

  • After starved in serum-free medium for 24h , A549 cells incubated with the indicated concentrations of AZD8055 for 3h , followed by 20-minute stimolation of 100ng/ml EGF

     
     

     

    Dr. Zhang of Tianjin Medical University. AZD8055 purchased from Selleck.

Purity & Quality Control

Choose Selective mTOR Inhibitors

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Notes:

2. For more details, such as half maximal inhibitory concentrations (IC50s) and working concentrations of each inhibitor, please click on the link of the inhibitor of interest.
3. "+" indicates inhibitory effect. Increased inhibition is marked by a higher "+" designation.
4. Orange "√" refers to compounds which do inhibitory effects on the related isoform, but without specific value.

Biological Activity

Description AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. Phase 1.
Features First drug to inhibit both types of mTOR protein.
Targets
mTOR (truncated) [1]
(MDA-MB-468 cells)
mTOR (full length) [1]
(MDA-MB-468 cells)
DNA-PK [1]
(MDA-MB-468 cells)
PI3Kδ [1]
(MDA-MB-468 cells)
PI3Kα [1]
(MDA-MB-468 cells)
0.13 nM 0.8 nM 1370 nM 3200 nM 3590 nM
In vitro

AZD8055 shows low activity (∼1,000-fold) against all PI3K isoforms (α, β, γ, δ) and other members of the PI3K-like kinase family (ATM and DNA-PK). AZD8055 inhibits the phosphorylation of mTORC1 (p70S6K and 4E-BP1) as well as phosphorylation of the mTORC2 (Akt) and downstream proteins. The rapamycin-resistant T37/46 phosphorylation sites on 4E-BP1 can be fully inhibited by AZD8055, resulting in significant inhibition of cap-dependent translation. AZD8055 potently inhibits proliferation in U87MG, A549 and H838 cells with IC50 of 53, 50 and 20 nM, respectively. AZD8055 also induces autophagy and increased LC3-II levels in H838 and A549 cells. [1] AZD8055 decreases AML blast cell proliferation and cell cycle progression, reduces the clonogenic growth of leukemic progenitors and induces caspase-dependent apoptosis in leukemic cells but not in normal immature CD34+ cells. [2] AZD8055 indicates inhibitory against the pediatric preclinical testing program (PPTP) cell lines with IC50 of 24.7 nM and induces significant differences in EFS distribution. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LU-139 MoC3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkX5TWM2OD1yLkCyN|MyKM7:TR?= NWLIVY1uW0GQR1XS
NCI-H720 NXG1fJpuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWn5bGZHUUN3ME2wMlAzQTN6IN88US=> MlHaV2FPT0WU
KARPAS-45 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVzJR|UxRTBwMEOzO|Eh|ryP NVv6SJhIW0GQR1XS
EW-3 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGHqNGNKSzVyPUCuNFQxPTZizszN M{H5dXNCVkeHUh?=
SBC-1 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXnjWHRSUUN3ME2wMlA1PThzIN88US=> MlXrV2FPT0WU
NCI-H209 NVy4U45sT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXHIVFFVUUN3ME2wMlA2PDV|IN88US=> M2HXU3NCVkeHUh?=
EoL-1-cell Mn7HS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUHJR|UxRTBwME[1Olkh|ryP NXu0fXBZW0GQR1XS
H9 M3HCW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIP5[W5KSzVyPUCuNFY3QTJizszN NILscWFUSU6JRWK=
AN3-CA NGjpVopIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYTUfGI{UUN3ME2wMlA4QTVzIN88US=> MWHTRW5ITVJ?
HH MnXJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFzIUmdKSzVyPUCuNFgxODlizszN MlHMV2FPT0WU
KU812 NW\XRmZUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVPqZZczUUN3ME2wMlA5ODR|IN88US=> Mlv6V2FPT0WU
DU-4475 NYXZXXI1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml;NTWM2OD1yLkC4NFQ4KM7:TR?= MWfTRW5ITVJ?
CTV-1 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn3DTWM2OD1yLkC4OlQyKM7:TR?= NH\XTGRUSU6JRWK=
BV-173 M2rGNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnTvTWM2OD1yLkC5NFYyKM7:TR?= NGX6enNUSU6JRWK=
ES5 NG\GTXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4PCU2lEPTB;MD6wPVM2KM7:TR?= M1LnUXNCVkeHUh?=
G-402 Mn7jS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlHnTWM2OD1yLkC5PVgh|ryP NHzEUGlUSU6JRWK=
MV-4-11 NHfVRWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWLGe5NmUUN3ME2wMlExOzR5IN88US=> NWnnRXNMW0GQR1XS
NB69 NWDye5Q3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{fBcWlEPTB;MD6xN|Uh|ryP NUXNbZBvW0GQR1XS
EGI-1 M2LlSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXrJR|UxRTBwMUO1N|Eh|ryP NX3C[49GW0GQR1XS
EW-18 NYSxZ|Z4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NY\LbWs6UUN3ME2wMlE1ODd|IN88US=> MY\TRW5ITVJ?
ME-180 NYPTWXJDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVnaU|dnUUN3ME2wMlE1PjhzIN88US=> MV7TRW5ITVJ?
D-283MED NF;5WpZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{HzSmlEPTB;MD6xOFczOyEQvF2= MYnTRW5ITVJ?
NB14 M4riXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV\lOFJyUUN3ME2wMlE3PDR5IN88US=> NV7lVppTW0GQR1XS
Ramos-2G6-4C10 MkjIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlXmTWM2OD1yLkG2PVg{KM7:TR?= M{juSHNCVkeHUh?=
NBsusSR MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVnJR|UxRTBwMUewNVkh|ryP MojPV2FPT0WU
LAMA-84 NX;0XWhRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{mwd2lEPTB;MD6xPFA{OiEQvF2= Mn\YV2FPT0WU
NCI-H1581 M3n0bWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{HNUWlEPTB;MD6xPFk3PiEQvF2= M1LmfHNCVkeHUh?=
A204 MnX0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4XkNWlEPTB;MD6xPFk4KM7:TR?= NUfqUnVFW0GQR1XS
A549 M3nGSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV7JR|UxRTBwMUm1OUDPxE1? NH75dYxUSU6JRWK=
CAL-51 NHTucnpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYDJR|UxRTBwMUm2OVEh|ryP MmDDV2FPT0WU
NKM-1 NGCxfVdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mmq0TWM2OD1yLkKwNFI6KM7:TR?= M4HQO3NCVkeHUh?=
LoVo M4rKbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkPITWM2OD1yLkKwNlAzKM7:TR?= NH7GOZlUSU6JRWK=
HAL-01 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnvZTWM2OD1yLkKwNlk4KM7:TR?= NELJWI1USU6JRWK=
HCC2218 MoPjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmH1TWM2OD1yLkKwOlg5KM7:TR?= M4P0Z3NCVkeHUh?=
BEN Mk\yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVXJR|UxRTBwMkGyPVMh|ryP MmTqV2FPT0WU
LC-2-ad MmGzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHq5RWhKSzVyPUCuNlIxQDRizszN NGPr[nhUSU6JRWK=
LXF-289 M1XRUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFnQSHNKSzVyPUCuNlUxPDFizszN M3TkO3NCVkeHUh?=
HGC-27 NXrZN|hZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoDSTWM2OD1yLkK2OFE1KM7:TR?= MmT6V2FPT0WU
LK-2 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1fnUWlEPTB;MD6yOlgzPCEQvF2= NYSyUZdxW0GQR1XS
CTB-1 NXf0bWd{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlfiTWM2OD1yLkK3NlE2KM7:TR?= MV;TRW5ITVJ?
697 NGHET5lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWnvS5F3UUN3ME2wMlI4OzZ5IN88US=> NXnpUFBzW0GQR1XS
ES7 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXvZNGVlUUN3ME2wMlI4Ozh|IN88US=> NUTlc4dYW0GQR1XS
MG-63 NUO4dllQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M33PTWlEPTB;MD6yO|QxPyEQvF2= NYrOdI9jW0GQR1XS
T47D MnHiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3rUbmlEPTB;MD6yO|Q4PSEQvF2= M3nxNXNCVkeHUh?=
RPMI-8226 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmXDTWM2OD1yLkK4OVg6KM7:TR?= MnTUV2FPT0WU
NCI-H292 M3u5NWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGL5RWZKSzVyPUCuNlkyPjRizszN NF7oT4FUSU6JRWK=
HT MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmLoTWM2OD1yLkK5N|gh|ryP NFWzbVFUSU6JRWK=
A3-KAW MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3LMO2lEPTB;MD6yPVc1KM7:TR?= M2TWUnNCVkeHUh?=
LB2241-RCC NGGyfoNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4r1SGlEPTB;MD6zNFE6QCEQvF2= NY\3RnFwW0GQR1XS
BB65-RCC NXLtOIJoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWTwO2NxUUN3ME2wMlMxQDB3IN88US=> M4ezdXNCVkeHUh?=
G-401 NVvUZ2VXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1HVeGlEPTB;MD6zNFk5PiEQvF2= MVfTRW5ITVJ?
TYK-nu MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3\pcmlEPTB;MD6zNVg3PCEQvF2= MYHTRW5ITVJ?
NCI-SNU-1 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUHifHdHUUN3ME2wMlMzOzNzIN88US=> MkH0V2FPT0WU
CHL-1 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2naWGlEPTB;MD6zNlcxOyEQvF2= MXPTRW5ITVJ?
P30-OHK MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWPJR|UxRTBwM{K3NFUh|ryP M1LyUHNCVkeHUh?=
PA-1 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mlr6TWM2OD1yLkOzOlE6KM7:TR?= NULTUW9xW0GQR1XS
LB1047-RCC MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUfJR|UxRTBwM{SwOFch|ryP M3:4TXNCVkeHUh?=
EM-2 M17rTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnPzTWM2OD1yLkO0NVg5KM7:TR?= MV7TRW5ITVJ?
VA-ES-BJ NUL3OnN3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2PaUWlEPTB;MD6zOFI3QCEQvF2= MkHrV2FPT0WU
NCI-H520 NWLXTmRLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2PYcWlEPTB;MD6zOFg{PCEQvF2= MlHKV2FPT0WU
ECC10 NVjSTJZ2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVjiSlZ{UUN3ME2wMlM2ODN3IN88US=> Mm\NV2FPT0WU
LNCaP-Clone-FGC MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXuyR5JiUUN3ME2wMlM2OzF2IN88US=> NH7IRYxUSU6JRWK=
ES8 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX:xRZNkUUN3ME2wMlM3PTV|IN88US=> MWXTRW5ITVJ?
HuO-3N1 NFW4W2ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{Tud2lEPTB;MD6zPFM4QSEQvF2= M2TkXHNCVkeHUh?=
L-363 Mmr6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1Hsd2lEPTB;MD6zPVAzPiEQvF2= MmnIV2FPT0WU
SK-LU-1 MknrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3jsbWlEPTB;MD6zPVIxQCEQvF2= NX\qWpBrW0GQR1XS
MCF7 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXi3Wll1UUN3ME2wMlQxPTNizszN Mkj6V2FPT0WU
HuO9 NVKxWmlVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWLJR|UxRTBwNEC2Olch|ryP MnvNV2FPT0WU
BHT-101 M{fReGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV;melJmUUN3ME2wMlQxPzh5IN88US=> M1nJSnNCVkeHUh?=
MZ2-MEL NXfVfod4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXTCNldyUUN3ME2wMlQxQDh{IN88US=> NWfOb21UW0GQR1XS
NOS-1 MnjNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWW1doJxUUN3ME2wMlQyODd2IN88US=> NWX5N|YyW0GQR1XS
NEC8 MorlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4HqVmlEPTB;MD60NVY6PSEQvF2= M1TkdXNCVkeHUh?=
COR-L88 NVvxe4xtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmTmTWM2OD1yLkSyNlY4KM7:TR?= MV\TRW5ITVJ?
MFM-223 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUfNfpFvUUN3ME2wMlQzPjF5IN88US=> MojWV2FPT0WU
HSC-3 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUnqOHdDUUN3ME2wMlQzQTB6IN88US=> NXf2XZZkW0GQR1XS
OVCAR-5 NXq2SIxzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmfWTWM2OD1yLkSyPVM{KM7:TR?= NF;6SHdUSU6JRWK=
MFE-280 NUX3NFdTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWXJR|UxRTBwNEOwN|Ih|ryP NHTyW4pUSU6JRWK=
KP-N-YS M{X3NWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWmwNVNTUUN3ME2wMlQ{ODdizszN MWfTRW5ITVJ?
EPLC-272H NHL6fXJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUOxOmlmUUN3ME2wMlQ{ODd5IN88US=> NGHlfo1USU6JRWK=
KE-37 M4nab2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWXJR|UxRTBwNEOyOFch|ryP Mm\XV2FPT0WU
HuH-7 NHrXVJJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVGwTnJZUUN3ME2wMlQ{Ozd{IN88US=> MnvQV2FPT0WU
CHP-212 NIPxdZVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2LpVGlEPTB;MD60N|Y6PyEQvF2= NGDzbFNUSU6JRWK=
SK-MES-1 NGLsUHRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{LXfGlEPTB;MD60N|g1QCEQvF2= MVrTRW5ITVJ?
SJSA-1 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWXMXlJPUUN3ME2wMlQ1PTV2IN88US=> MVnTRW5ITVJ?
QIMR-WIL MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYXJR|UxRTBwNEWxOUDPxE1? MU\TRW5ITVJ?
ES4 NWDWb|RET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYjJR|UxRTBwNEWzN|Yh|ryP MmHyV2FPT0WU
TI-73 M165U2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUDJR|UxRTBwNEW2PFYh|ryP MVvTRW5ITVJ?
COLO-684 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV\VPZNwUUN3ME2wMlQ2PzJ4IN88US=> NXuzT4d{W0GQR1XS
GCIY NHjHbXdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn\PTWM2OD1yLkS1O|U1KM7:TR?= NYS4dWhFW0GQR1XS
JVM-3 M4rGRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3n1UGlEPTB;MD60OlI6OiEQvF2= MlP0V2FPT0WU
MPP-89 NUTVUWNUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M33wUmlEPTB;MD60Olk1PyEQvF2= MlL0V2FPT0WU
M14 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEHjOlRKSzVyPUCuOFcyPyEQvF2= NYHqbmJZW0GQR1XS
SW962 M1;1bWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NULWUXk3UUN3ME2wMlQ4OTlizszN M3XGcHNCVkeHUh?=
VM-CUB-1 MmrWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mmq0TWM2OD1yLkS3Nlg3KM7:TR?= NHTWeIZUSU6JRWK=
A101D NVTGRnd2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mo\JTWM2OD1yLkS4PVY5KM7:TR?= MW\TRW5ITVJ?
DB NIXVW4dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUHCc4plUUN3ME2wMlUxODJzIN88US=> MULTRW5ITVJ?
KNS-62 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVPJR|UxRTBwNUC1Olgh|ryP M2m2c3NCVkeHUh?=
NCI-H460 NGTN[|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnLNTWM2OD1yLkWwOVk4KM7:TR?= MYnTRW5ITVJ?
MDA-MB-361 NV3vdYExT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MY\JR|UxRTBwNUC4O{DPxE1? NIXvVGFUSU6JRWK=
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SK-PN-DW NGnN[VZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NET0RphKSzVyPUGuO|U3QDJizszN Mn\sV2FPT0WU
5637 Mm\DS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml3VTWM2OD1zLke3NlY5KM7:TR?= MWXTRW5ITVJ?
BT-474 NVTpbGh2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGTXeXFKSzVyPUGuO|gyPzFizszN MnnmV2FPT0WU
KM12 MkjYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGfleY1KSzVyPUGuO|k4PDFizszN MUHTRW5ITVJ?
COLO-680N MkX3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUHGOFViUUN3ME2xMlgzOzhzIN88US=> MWnTRW5ITVJ?
Saos-2 NVfFcldUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX7JR|UxRTFwOEK0OFUh|ryP NUD5N5J2W0GQR1XS
CAL-54 MmrOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX3JR|UxRTFwOEOzNVMh|ryP MnmzV2FPT0WU
U-118-MG MmTYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mo\4TWM2OD1zLki0OlIzKM7:TR?= M{PQN3NCVkeHUh?=
TGBC24TKB MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUnJR|UxRTFwOEW1NFEh|ryP MVHTRW5ITVJ?
NCI-H2052 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1viTGlEPTB;MT64OlQyKM7:TR?= NXHheohSW0GQR1XS
BB30-HNC M1;kSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXXJR|UxRTFwOE[0OlIh|ryP NEXFblhUSU6JRWK=
KNS-81-FD M1j3TGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX3F[4NKUUN3ME2xMlg6OTZ3IN88US=> Mkm0V2FPT0WU
HN MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIDxXoZKSzVyPUGuPFk2PDVizszN NEX6eY1USU6JRWK=
ACHN MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXSzd2w{UUN3ME2xMlkxPzh4IN88US=> NVfr[oJrW0GQR1XS
HSC-2 MkjkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2S2PWlEPTB;MT65NVA6PiEQvF2= MnPXV2FPT0WU
HCC2998 MojqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUXJR|UxRTFwOUOzNlUh|ryP M4LhSHNCVkeHUh?=
HEL Ml;PS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4XNRmlEPTB;MT65OFE5PCEQvF2= MWXTRW5ITVJ?
SW684 NWnFb5BXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYHFdZI2UUN3ME2xMlk4OzdzIN88US=> NGjDeHdUSU6JRWK=
NCI-H1693 NHHoSIRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2rNeWlEPTB;MT65PFA5PiEQvF2= M324eXNCVkeHUh?=
8-MG-BA NVvoXo1yT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXnyVlRtUUN3ME2xMlk5OjFizszN NUHxbJhTW0GQR1XS
OVCAR-8 NInJWmpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXvJR|UxRTJwMECyPVUh|ryP MoXOV2FPT0WU
DSH1 NGj1dndIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWTJR|UxRTJwMEG3O|Qh|ryP NGDud3BUSU6JRWK=
KYSE-520 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NInzW3BKSzVyPUKuNFIxPDlizszN MnjuV2FPT0WU
GB-1 NYjoO2gxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mo\JTWM2OD1{LkCyOVc4KM7:TR?= MVjTRW5ITVJ?
RCC10RGB MmDWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXvLdJdZUUN3ME2yMlA1QDdzIN88US=> NH3NcItUSU6JRWK=
SW954 NIHu[I1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2HsV2lEPTB;Mj6wOVA{PiEQvF2= MVvTRW5ITVJ?
IST-MES1 Mn7aS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEnV[ppKSzVyPUKuNFU2PjhizszN NGX5[XdUSU6JRWK=
AU565 NYjyPGZ6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYLJR|UxRTJwMEW5NFUh|ryP MortV2FPT0WU
CW-2 NWryXVB6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYHPUW5wUUN3ME2yMlA3QDd3IN88US=> NUTyNZRSW0GQR1XS
TE-1 NUnw[GlHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV\JR|UxRTJwMEmzO|ch|ryP MUPTRW5ITVJ?
KP-4 MlHmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF;VcJZKSzVyPUKuNVA{OTJizszN M4\5dnNCVkeHUh?=
SNU-C2B M1XGOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkDoTWM2OD1{LkGwPFI{KM7:TR?= Mmj6V2FPT0WU
NCI-H1395 NEDiZ|NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVrYeYZvUUN3ME2yMlExQTh4IN88US=> NFv2WoFUSU6JRWK=
CAS-1 NWDFXHRDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYT6OJByUUN3ME2yMlEyPTR4IN88US=> NV6yeJhxW0GQR1XS
NCI-H1155 NVu1UGt6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M17PO2lEPTB;Mj6yNlY6KM7:TR?= MVvTRW5ITVJ?
SK-MEL-1 NXjER285T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUDvflZ2UUN3ME2yMlI5OThzIN88US=> M3H0W3NCVkeHUh?=
KINGS-1 NGr3SoNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVnCe5dtUUN3ME2yMlMxODh6IN88US=> MWrTRW5ITVJ?
ESS-1 M1qxfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1z1[GlEPTB;Mj6zNFUyKM7:TR?= MWDTRW5ITVJ?
PANC-10-05 NWfyR3VGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFTIeolKSzVyPUKuN|cxOjRizszN NWnLTItDW0GQR1XS
HT-1376 M2DTcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWjJWo1yUUN3ME2yMlM5PzB6IN88US=> NFHTenlUSU6JRWK=
LS-513 NUPVV3dzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4fy[mlEPTB;Mj6zPFg4PCEQvF2= NIm3WlRUSU6JRWK=
KNS-42 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVLSZZF1UUN3ME2yMlM6OTB3IN88US=> NHzJWIpUSU6JRWK=
639-V M2PjVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3LDdmlEPTB;Mj60Nlc2QCEQvF2= NF7OcmtUSU6JRWK=
SJRH30 NUDFdZR{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2\4[2lEPTB;Mj60OVE3OSEQvF2= NYn1enU{W0GQR1XS
SW1783 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYHJR|UxRTJwNEewO|gh|ryP MYjTRW5ITVJ?
NB6 M3z2Z2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M13vS2lEPTB;Mj60PFI5KM7:TR?= MmXLV2FPT0WU
SK-MEL-30 NIHSS21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWPJR|UxRTJwNEi4N{DPxE1? NUj1VnpGW0GQR1XS
HCE-4 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3HtbmlEPTB;Mj60PVQxOiEQvF2= NUi3cY1yW0GQR1XS
NB10 NEnzT5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV\leVJGUUN3ME2yMlQ6PDJ7IN88US=> MmXlV2FPT0WU
H-EMC-SS MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYHJR|UxRTJwNUS2OUDPxE1? NW\WSll[W0GQR1XS
SK-HEP-1 M1vsO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHnEWZdKSzVyPUKuOVUzOzZizszN M2XoUHNCVkeHUh?=
HCC1395 MnOxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnLHTWM2OD1{LkW1OlU2KM7:TR?= NFvpTppUSU6JRWK=
HuCCT1 M4fJd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWPJR|UxRTJwNU[zPUDPxE1? MV3TRW5ITVJ?
SiHa M2rse2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIn2dItKSzVyPUKuOVg4OTlizszN MoW5V2FPT0WU
EFO-21 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX;JV5FKUUN3ME2yMlU5PzZ2IN88US=> NYi5TWl{W0GQR1XS
NCI-H1666 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmjPTWM2OD1{LkW5NFc3KM7:TR?= NGDJNWZUSU6JRWK=
UMC-11 MlzmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHvNWFVKSzVyPUKuOVk5PjhizszN NGfyeZNUSU6JRWK=
COLO-679 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYL1bplYUUN3ME2yMlYyOjN|IN88US=> MU\TRW5ITVJ?
A388 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIXCRmRKSzVyPUKuOlUzPTNizszN M2S3WXNCVkeHUh?=
LB2518-MEL NEPmNFlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIT0d2VKSzVyPUKuOlU1ODhizszN MV;TRW5ITVJ?
EW-13 NYi1cFA2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX\JR|UxRTJwNki3Olgh|ryP MUHTRW5ITVJ?
MLMA M1OxN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH7WU5NKSzVyPUKuOlg5PTZizszN MVnTRW5ITVJ?
HOP-92 MnnIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWHqd5l7UUN3ME2yMlcxOTFizszN MoXRV2FPT0WU
NOMO-1 Mn;LS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF;6eHdKSzVyPUKuO|E1PDJizszN NWTV[XZHW0GQR1XS
NCI-H810 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlvxTWM2OD1{LkeyNVk5KM7:TR?= NXzN[2k{W0GQR1XS
769-P NH:2VpZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4C5T2lEPTB;Mj63NlI5KM7:TR?= NIThS2xUSU6JRWK=
SAS NVjMTYI{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1HuU2lEPTB;Mj63OlUh|ryP NH;4e4pUSU6JRWK=
M059J M13sWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{XKU2lEPTB;Mj63PFMyQCEQvF2= NYGyflByW0GQR1XS
RD M3L5TGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4TVU2lEPTB;Mj63PVQ4OSEQvF2= M2m3c3NCVkeHUh?=
GT3TKB MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXHJR|UxRTJwOEO0N|Mh|ryP MX7TRW5ITVJ?
MZ7-mel NVTISVRTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEK1N2VKSzVyPUKuPFY2ODJizszN Ml;iV2FPT0WU
KYSE-510 NUXWTVBHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1nRfWlEPTB;Mj64PVUxPCEQvF2= NHyzOm9USU6JRWK=
NCI-N87 NUTlXXcyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVXtXolRUUN3ME2yMlkyQDB5IN88US=> M{LaTnNCVkeHUh?=
MKN1 MnzpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnyyTWM2OD1|LkCwNVY{KM7:TR?= M3PNPHNCVkeHUh?=
NCI-H2170 NVWwUJh5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkXhTWM2OD1|LkCwOFUyKM7:TR?= MYTTRW5ITVJ?
OVCAR-3 NV63RWpjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmHuTWM2OD1|LkCyNVE2KM7:TR?= MVPTRW5ITVJ?
KLE NFHYN2dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3\MZ2lEPTB;Mz6wOlgyOiEQvF2= MXrTRW5ITVJ?
LB771-HNC Mn75S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2TjR2lEPTB;Mz6wPVM1OyEQvF2= MkjNV2FPT0WU
MC116 M3ntcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3PQT2lEPTB;Mz6xNVEyOiEQvF2= MofUV2FPT0WU
SCC-9 MlvES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXr5dIIyUUN3ME2zMlEyOzZ4IN88US=> M{SyS3NCVkeHUh?=
U031 NH3Jb3VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUXHV|doUUN3ME2zMlEzPDJ|IN88US=> NVvDNoVXW0GQR1XS
LCLC-103H NIHSdHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXfJR|UxRTNwMkO4PVQh|ryP M3[0PHNCVkeHUh?=
U-266 M2C1U2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NILhZZVKSzVyPUOuNlc3OzNizszN NEPGPHFUSU6JRWK=
JVM-2 NWXkVphET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoSxTWM2OD1|LkOyNVY1KM7:TR?= NGPHUHRUSU6JRWK=
UACC-257 NGewe45Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlvLTWM2OD1|LkS2OFMyKM7:TR?= MonDV2FPT0WU
LB996-RCC M{DpbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{n4emlEPTB;Mz60OlUxOiEQvF2= NGS4WYxUSU6JRWK=
BT-549 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUDJR|UxRTNwNUCyNFkh|ryP MUnTRW5ITVJ?
NCI-H526 NUezcodqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnT6TWM2OD1|LkW0NVU5KM7:TR?= MonVV2FPT0WU
RH-18 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYPJR|UxRTNwNkG3PVgh|ryP MVfTRW5ITVJ?
SW626 Ml;YS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUDUUXBbUUN3ME2zMlcyOTJ6IN88US=> NIjHfHNUSU6JRWK=
SK-N-AS NV\yU4hxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlTZTWM2OD1|LkezNlQ4KM7:TR?= NEPFNpZUSU6JRWK=
IGR-1 NFLLTpZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3LMOWlEPTB;Mz63OFI1QCEQvF2= NHHBNolUSU6JRWK=
NCI-H2291 NX:0eIE1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4jxTWlEPTB;Mz63OVM{PiEQvF2= MXHTRW5ITVJ?
NCI-H441 NHPz[ZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHjsZmZKSzVyPUOuPFI1OzNizszN M2Xjc3NCVkeHUh?=
D-247MG NXLUVGZ6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1\nSmlEPTB;Mz64OlA{OSEQvF2= NIrnS|FUSU6JRWK=
COLO-320-HSR MoTlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUjJR|UxRTNwOUGyO|Eh|ryP MX3TRW5ITVJ?
A253 Mm\jS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1HORWlEPTB;Mz65NVQ{PiEQvF2= M4jMVXNCVkeHUh?=
HT55 MkjxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYLJR|UxRTNwOUG3O|Mh|ryP M4fre3NCVkeHUh?=
MZ1-PC M1HXOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MojhTWM2OD1|Lkm1PFY1KM7:TR?= MkDOV2FPT0WU
RERF-LC-MS NU[wT2MxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1LmO2lEPTB;Mz65OlUzPSEQvF2= MUXTRW5ITVJ?
SF268 NUTWZ29xT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYPJR|UxRTRwMUK4PFEh|ryP M2XVS3NCVkeHUh?=
RMG-I NXfxNHZkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEXPRlFKSzVyPUSuNlE1PTRizszN MYDTRW5ITVJ?
CFPAC-1 M3;DNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXj0[5lvUUN3ME20MlIyPjh3IN88US=> NGq1TJNUSU6JRWK=
S-117 NHu5RnpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnzxTWM2OD12LkKyNFI1KM7:TR?= NUK2fHBZW0GQR1XS
NCI-H2122 NGHiSotIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXzJR|UxRTRwM{C5N|Eh|ryP M3L1ZXNCVkeHUh?=
J82 MlrZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3HxdWlEPTB;ND6zN|Q1OSEQvF2= NH3jdWNUSU6JRWK=
CAL-72 NX\aOWh[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MY\JR|UxRTRwM{W0O|ch|ryP MlvVV2FPT0WU
GP5d M3zFN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmK5TWM2OD12LkSxNlk5KM7:TR?= M2jaWnNCVkeHUh?=
UACC-62 NYK0bW9HT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mnz4TWM2OD12LkS2OVQ2KM7:TR?= MXXTRW5ITVJ?
MS-1 NFXwdGNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmLYTWM2OD12LkS5NVE2KM7:TR?= MlrQV2FPT0WU
RCM-1 NX;EVHdMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWPJR|UxRTRwNUK5NkDPxE1? MorYV2FPT0WU
IST-SL1 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4PjcGlEPTB;ND62OVM2QSEQvF2= MYjTRW5ITVJ?
SW756 NUfoV|B[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml33TWM2OD12Lk[5NVM2KM7:TR?= Ml;0V2FPT0WU
HCC38 NEns[5lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4XI[WlEPTB;ND63NVE6PyEQvF2= MYHTRW5ITVJ?
YAPC MnvQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1nQbGlEPTB;ND63NVMyOyEQvF2= NUOy[|dEW0GQR1XS
DK-MG NEfPO3lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVvRNYhTUUN3ME20Mlc5OTF{IN88US=> NHjTPHpUSU6JRWK=
HCC1937 MnrxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXHJR|UxRTRwOEWxNlUh|ryP MUfTRW5ITVJ?
OAW-28 NF7NfpVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUjiRWFzUUN3ME20MlkzPDN3IN88US=> MWTTRW5ITVJ?
MEG-01 M{jpNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHK5[nZKSzVyPUWuNFg{OjhizszN MmHSV2FPT0WU
GI-1 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX3JR|UxRTVwMkO0PFYh|ryP M37jWHNCVkeHUh?=
FADU MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFT2Vm1KSzVyPUWuNlc5OzdizszN MVfTRW5ITVJ?
CAPAN-1 NX61V3VRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2fZOWlEPTB;NT6zPVQ{OiEQvF2= NITScnZUSU6JRWK=
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DLD1 M3XDeWZ2dmO2aX;uJGF{e2G7 NVO3cWNXOSEQvF2= MkXJO|IhcA>? MYHpcoR2[2W|IHH1eI9xcGGpeTD0c{Bkd26oZYKgZ5l1d3C{b4TlZ5Rqd25? NYTuUWJwOjF7NEmxNlE>
Rh41 MlPVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1HLXGlEPTB;M{CuO{BvVQ>? MorMNlE{Ozd4N{m=
Rh18 NF3Ue|lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUfZOYoxUUN3ME20NU41KG6P MoHFNlE{Ozd4N{m=
Rh30 NWXZfFlzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Moq1TWM2OD1zNDDuUS=> M3HkW|IyOzN5Nke5
BT-12 NEDUZWpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV7kWWZ2UUN3ME2yOE44KG6P MmCzNlE{Ozd4N{m=
CHLA-266 NEC1bWtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWPnT2w2UUN3ME2xMlEhdk1? NGX1WW8zOTN|N{[3PS=>
TC-71 NGL6eIpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVniR2pmUUN3ME20Ok42KG6P NHHzbHEzOTN|N{[3PS=>
CHLA-9 NUXKdXVuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWnyR5BXUUN3ME2xOU4{KG6P MkTzNlE{Ozd4N{m=
CHLA-10 NGqycplIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnXLTWM2OD1|Lkmgcm0> NFT1WYMzOTN|N{[3PS=>
CHLA-258 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3\UPGlEPTB;MUCuOEBvVQ>? MlywNlE{Ozd4N{m=
GBM2 M17XVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmjCTWM2OD12MD63JI5O NFLNO|gzOTN|N{[3PS=>
NB-1643 MkfHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVzJR|UxRTN|Lkigcm0> NVXDSoY1OjF|M{e2O|k>
NB-EBc1 NILkRXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX\JR|UxRTJ5LkGgcm0> M2P2elIyOzN5Nke5
CHLA-90 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MofvTWM2OD1|ND61JI5O NWDxNJVuOjF|M{e2O|k>
CHLA-136 NIfMUJdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVW0NohoUUN3ME2xOlIvPiCwTR?= MUGyNVM{PzZ5OR?=
NALM-6 NYC0bXZJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYH6c2xmUUN3ME2zNk46KG6P NIDZNlAzOTN|N{[3PS=>
COG-LL-317 Mmr3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWDJR|UxRTFwMzDuUS=> MoLGNlE{Ozd4N{m=
RS4;11 NV\4cWFbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEDoTHdKSzVyPUO1JI5O NWTLTIFTOjF|M{e2O|k>
MOLT-4 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXXqXIV2UUN3ME24MlYhdk1? NIS2RmczOTN|N{[3PS=>
CCRF-CEM MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV\JR|UxRTRwMTDuUS=> MU[yNVM{PzZ5OR?=
Kasumi-1 NYe0ZXV{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoPRTWM2OD1zLkSgcm0> Mkn5NlE{Ozd4N{m=
Karpas-299 NIDjfHRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXnFSo9bUUN3ME2yNU44KG6P MliwNlE{Ozd4N{m=
Ramos-RA1 MlXaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml7wTWM2OD16LkKgcm0> MnrFNlE{Ozd4N{m=
H838 MXXGeY5kfGmxbjDBd5NigQ>? MnfjNUDPxE1? MUm0PEBp M2PnVYlv\HWlZYOgZ4VtdCCjdYTvdIhi\3l? NHv3[IczODN4NEGxNy=>
H838 M3PZcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4fNclAuOyEQvF2= MV63NkBp M4f3[4lv\HWlZYOg[5Jwf3SqIHnubIljcXSrb36gZY5lKGG3dH;wbIFogQ>? NV3IO3FpOjByMki4OVQ>
A549  NFfrfmpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYmwMVMh|ryP MUK3NkBp NHHR[IFqdmS3Y3XzJIdzd3e2aDDpcohq[mm2aX;uJIFv\CCjdYTvdIhi\3l? M2fmPFIxODJ6OEW0

... Click to View More Cell Line Experimental Data

In vivo AZD8055 inhibits the pS6 and pAkt in U87MG and A549 xenografts at 2.5/10 mg/kg, which leads to tumor growth inhibition. AZD8055 shows significant antitumor activity in many xenografts, including U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA at a dose of 10/20 mg/kg. [1] AZD8055 induces ~40% reduction in tumour volume, accompanied by ablation of phosphorylation of Akt, S6K and SGK protein kinases. Administration of AZD8055 (5mg/kg, Bid) and SAHA (100 mg/kg/d) results in complete tumor growth inhibition in PTEN+/−LKB1+/hypo xenografts without side effects on mice by inhibition of mTORC1 and mTORC2 signaling. [4]

Protocol

Kinase Assay
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Cell-based determinations of mTOR inhibition:

A high-throughput screening cell-based assay is developed using MDA-MB-468 cells to detect mTORC1 and mTORC2 activity. Cells are exposed for 2 hours to increasing concentrations of AZD8055. At the end of the incubation period, cells are fixed, washed, and probed with antibodies against S473 pAkt or against S235/236 phosphorylated S6 (pS6). Levels of phosphorylation are assessed using an Acumen laser scanning cytometer.
Cell Research
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  • Cell lines: U87MG, A549 and H838 cells
  • Concentrations: 1 nM - 1 μM
  • Incubation Time: 72 to 96 hours
  • Method: Cells are exposed to AZD8055 for 72 to 96 hours and stained for cell nuclei (0.03 mg/mL Hoechst 33342) and acidic vesicles (1 μg/mL acridine orange). Images are captured at 450 and 536 nm on an ArrayScan II platform, and the percentage of acidic vesicles and the number of cells are quantified. For LC3 assessment, cells are exposed to e64d/pepstatin (10 μg/mL) for 30 to 90 min before incubation with AZD8055. Cells are lysed on ice and analyzed by immunoblotting.
    (Only for Reference)
Animal Research
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  • Animal Models: U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA U87-MG and A549 are established in pathogen-free, female nude mice (nu/nu:Alpk).
  • Formulation: Dissolved in captisol (CyDex, pH 3.0) and diluted to a final captisol concentration of 30% (w/v)
  • Dosages: 2.5-20 mg/kg
  • Administration: Oral gavage once or twice daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 50 mg/mL (107.4 mM) warming
Ethanol 3 mg/mL (6.44 mM)
Water <1 mg/mL
In vivo 4% DMSO+30% PEG 300+ddH2O 5mg/mL

* 1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 465.54
Formula

C25H31N5O4

CAS No. 1009298-09-2
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01316809 Completed Glioblastoma Multiforme|Anaplastic Astrocytoma|Anaplastic Oligodendroglioma|Malignant Glioma|Brainstem Glioma National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) March 2011 Phase 1
NCT00999882 Completed Cancer|Advanced Hepatocellular Carcinoma AstraZeneca October 2009 Phase 1
NCT00973076 Completed Cancer|Solid Tumors|Advanced Solid Malignancies AstraZeneca August 2009 Phase 1
NCT00731263 Completed Solid Tumors AstraZeneca July 2008 Phase 1
NCT01194193 Withdrawn Cancer|Advanced Solid Tumours|Lymphomas AstraZeneca null Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID