AZD8055

Catalog No.S1555

AZD8055 Chemical Structure

Molecular Weight(MW): 465.54

AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. Phase 1.

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In DMSO USD 156 In stock
USD 120 In stock
USD 370 In stock
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Cited by 38 Publications

8 Customer Reviews

  • PC3-LN4 cells were treated with GSK690693 (5 uM) alone, or in combination with PP242 (2 uM) or AZD8055 (1 uM) for 24 h, and immunoblotting performed.

    Cancer Res 2013 73(11), 3402-11. AZD8055 purchased from Selleck.

    HUVEC were treated with either RAD001 or AZD8055 before PA treatment. p53, GPX1, p53 acetylation, and subcellular PTEN were analyzed with corresponding antibodies. The blot is a representative of the results of three experiments. Values (mean盨D) are expressed as fold over untreated control. *p<0.05 versus their corresponding control groups in the one-way ANOVA.

    Antioxid Redox Signal 2014 20(9), 1382-95. AZD8055 purchased from Selleck.

  • (A) HCT116 or MDA-MB-468 cells were treated with 500 nM AZD8055 for the indicated times. Cell lysates were immunoblotted with the indicated antibodies. (B) HCT116 or MDA-MB-468 cells were treated with 50 nM rapamycin. 500 nM AZD8055, 1 uM MK2206 or dimethyl sulfoxide (DMSO) as control for 12 h. Cell lysates were immunoblotted with the indicated antibodies.

    Oncotarget 2014 5(15), 6015-27. AZD8055 purchased from Selleck.

    CSCs+24/44/ESA at 5,000 cells per well with stem cell culture media in triplicate in an ultra-low adhesion plate were treated with 5 umol/L BMS-777607, 1 umol/L AZD8055 alone, or in their different combinations. Cells were cultured for 72 hours. Cell viability was determined by the MTS assay. Percentages of polyploid cells were determined by counting 300 cells from two different regions. Results shown here were from one of two experiments with similar results.

    Mol Cancer Ther 2014 13(1), 37-48. AZD8055 purchased from Selleck.

  • mTOR kinase inhibitor AZD8055 activates PI3K accompanied with induction of expression of EGFR, HER2, HER3 and IRS1. Serum-deprived CHO-EGFP-AKT cells were incubated with 50 nM AZD8055 for 24 hr. The EGFP signal was detected using confocal microscopy. The white arrows indicate EGFP-AKT located on cellular membrane.

    Int J Cancer 2014 135(10), 2462-74. AZD8055 purchased from Selleck.

    mTOR, P70S6K1, 4E-BP1 and PCNA activation in the basilar artery of SAH. Top, Western blotting bands of antibodies, bottom, density of the protein band from each group. A, mTOR activation in the basilar artery demonstrated an elevated expression of mTOR on day 7 following SAH in SAH and SAH+DMSO animals. The mTOR inhibitors, Rapamycin and AZD8055 reduced the enhanced expression of mTOR (pb0.05, ANOVA). B. P70S6K1 activation. C. 4E-BP1 activation. D. PCNA activation. Rapamycin and AZD8055 significantly decreased the expression of P70S6K1, 4E-BP1 and PCNA compared with the SAH and SAH+DMSO (pb0.05, respectively; ANOVA). There was no significant difference between the two inhibitors in mTOR, P70S6K1, 4E-BP1 and PCNA expression.

    Exp Neurol 2012 233, 799-806. AZD8055 purchased from Selleck.

  • Induction of TCTP expression through 5-FU or oxaliplatin is inhibited by mTOR kinase inhibitors. HCT116 cells were treated for 48 h with 5-FU or oxaliplatin at the indicated concentrations. Where indicated, AZD 8055 (100 nM) or PP242 (1 μM) were added. TCTP levels were assessed by western blotting; signals were quantified and normalised against α-tubulin as a loading control. As a readout of mTOR activity, phosphorylation of the ribosomal protein S6 at Ser 240/244 was assessed using a phospho-specific antibody. a. Representative western blots demonstrating the alteration of the levels of TCTP protein and of phospho-ribosomal protein S6, respectively, in relation to the drug concentration.

    Cell Commun Signal, 2017.. AZD8055 purchased from Selleck.

    After starved in serum-free medium for 24h , A549 cells incubated with the indicated concentrations of AZD8055 for 3h , followed by 20-minute stimolation of 100ng/ml EGF

     
     

     

    Dr. Zhang of Tianjin Medical University. AZD8055 purchased from Selleck.

Purity & Quality Control

Choose Selective mTOR Inhibitors

Biological Activity

Description AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. Phase 1.
Features First drug to inhibit both types of mTOR protein.
Targets
mTOR (truncated) [1]
(MDA-MB-468 cells)
mTOR (full length) [1]
(MDA-MB-468 cells)
0.13 nM 0.8 nM
In vitro

AZD8055 shows low activity (∼1,000-fold) against all PI3K isoforms (α, β, γ, δ) and other members of the PI3K-like kinase family (ATM and DNA-PK). AZD8055 inhibits the phosphorylation of mTORC1 (p70S6K and 4E-BP1) as well as phosphorylation of the mTORC2 (Akt) and downstream proteins. The rapamycin-resistant T37/46 phosphorylation sites on 4E-BP1 can be fully inhibited by AZD8055, resulting in significant inhibition of cap-dependent translation. AZD8055 potently inhibits proliferation in U87MG, A549 and H838 cells with IC50 of 53, 50 and 20 nM, respectively. AZD8055 also induces autophagy and increased LC3-II levels in H838 and A549 cells. [1] AZD8055 decreases AML blast cell proliferation and cell cycle progression, reduces the clonogenic growth of leukemic progenitors and induces caspase-dependent apoptosis in leukemic cells but not in normal immature CD34+ cells. [2] AZD8055 indicates inhibitory against the pediatric preclinical testing program (PPTP) cell lines with IC50 of 24.7 nM and induces significant differences in EFS distribution. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LU-139 NYrnd4pQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUj6V|FGUUN3ME2wMlAzOzNzIN88US=> NVrTd|dYW0GQR1XS
NCI-H720 MmrhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXnhXYZvUUN3ME2wMlAzQTN6IN88US=> MUDTRW5ITVJ?
KARPAS-45 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2\ldWlEPTB;MD6wN|M4OSEQvF2= NWXrR5ZWW0GQR1XS
EW-3 MkTaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml71TWM2OD1yLkC0NFU3KM7:TR?= MlrTV2FPT0WU
SBC-1 M2nOTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVnrTWNYUUN3ME2wMlA1PThzIN88US=> M{nVT3NCVkeHUh?=
NCI-H209 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHTO[HhKSzVyPUCuNFU1PTNizszN NIrHRpZUSU6JRWK=
EoL-1-cell M1rjWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NG\XNHJKSzVyPUCuNFY2PjlizszN NV;PPFBsW0GQR1XS
H9 M{fmeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmqzTWM2OD1yLkC2OlkzKM7:TR?= NFjReYZUSU6JRWK=
AN3-CA MlfzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnfNTWM2OD1yLkC3PVUyKM7:TR?= M1SzZXNCVkeHUh?=
HH MlzHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2HTeGlEPTB;MD6wPFAxQSEQvF2= NHL5SZBUSU6JRWK=
KU812 NGP5OZhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mm\WTWM2OD1yLkC4NFQ{KM7:TR?= M{XvV3NCVkeHUh?=
DU-4475 MmHDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlzhTWM2OD1yLkC4NFQ4KM7:TR?= Mlv4V2FPT0WU
CTV-1 M3\ycGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkjHTWM2OD1yLkC4OlQyKM7:TR?= MX3TRW5ITVJ?
BV-173 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWrJR|UxRTBwMEmwOlEh|ryP MorTV2FPT0WU
ES5 NW\NUmhJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NI\Hfo5KSzVyPUCuNFk{PSEQvF2= M3HjTXNCVkeHUh?=
G-402 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVPJR|UxRTBwMEm5PEDPxE1? M1G4cXNCVkeHUh?=
MV-4-11 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4X4UmlEPTB;MD6xNFM1PyEQvF2= MVjTRW5ITVJ?
NB69 NEjI[HlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkXHTWM2OD1yLkGzOUDPxE1? M2O0UHNCVkeHUh?=
EGI-1 M1f5c2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHnMc|hKSzVyPUCuNVM2OzFizszN MYLTRW5ITVJ?
EW-18 NF;hPFRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn35TWM2OD1yLkG0NFc{KM7:TR?= NYO4XXE4W0GQR1XS
ME-180 M{Pmbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWTJR|UxRTBwMUS2PFEh|ryP NX7qPXhTW0GQR1XS
D-283MED MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVL6OHFHUUN3ME2wMlE1PzJ|IN88US=> MmG2V2FPT0WU
NB14 NUXOXFVGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFXrSZpKSzVyPUCuNVY1PDdizszN NV7BfXNCW0GQR1XS
Ramos-2G6-4C10 Mof4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYLJR|UxRTBwMU[5PFMh|ryP Moj2V2FPT0WU
NBsusSR MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFfiZXRKSzVyPUCuNVcxOTlizszN MkXuV2FPT0WU
LAMA-84 M{DMOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVnJR|UxRTBwMUiwN|Ih|ryP M1m1[HNCVkeHUh?=
NCI-H1581 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXvJR|UxRTBwMUi5OlYh|ryP NHLSVZFUSU6JRWK=
A204 MlHXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1npfmlEPTB;MD6xPFk4KM7:TR?= M1TiN3NCVkeHUh?=
A549 MnL4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGi1[21KSzVyPUCuNVk2PSEQvF2= M1THPHNCVkeHUh?=
CAL-51 NGjX[phIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4\rcGlEPTB;MD6xPVY2OSEQvF2= MYjTRW5ITVJ?
NKM-1 NGnLdpBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2C4cWlEPTB;MD6yNFAzQSEQvF2= NWrZO2tvW0GQR1XS
LoVo MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVzqe|h5UUN3ME2wMlIxOjB{IN88US=> NXPicXZGW0GQR1XS
HAL-01 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFPKUYVKSzVyPUCuNlAzQTdizszN MUPTRW5ITVJ?
HCC2218 MnLxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYrJR|UxRTBwMkC2PFgh|ryP MYjTRW5ITVJ?
BEN MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYW5RmFnUUN3ME2wMlIyOjl|IN88US=> M4T0PHNCVkeHUh?=
LC-2-ad NYDpcGNLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoPhTWM2OD1yLkKyNFg1KM7:TR?= NX32UIplW0GQR1XS
LXF-289 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2DoSmlEPTB;MD6yOVA1OSEQvF2= NVPRSGFIW0GQR1XS
HGC-27 NHPMNoZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVP4fVh5UUN3ME2wMlI3PDF2IN88US=> NYC3eVRrW0GQR1XS
LK-2 MnPHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYf3Rod5UUN3ME2wMlI3QDJ2IN88US=> NXq4cJVnW0GQR1XS
CTB-1 MnvwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXzHb3VIUUN3ME2wMlI4OjF3IN88US=> MWrTRW5ITVJ?
697 NFHSN5dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEn0VmNKSzVyPUCuNlc{PjdizszN M1\kOnNCVkeHUh?=
ES7 MkjOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4rHb2lEPTB;MD6yO|M5OyEQvF2= NHn5[5RUSU6JRWK=
MG-63 MmrpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWTJR|UxRTBwMke0NFch|ryP MYfTRW5ITVJ?
T47D MoHxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoXtTWM2OD1yLkK3OFc2KM7:TR?= M3T4VnNCVkeHUh?=
RPMI-8226 NGLOfItIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXLpWlBwUUN3ME2wMlI5PTh7IN88US=> NEm5RnNUSU6JRWK=
NCI-H292 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWL5e|NiUUN3ME2wMlI6OTZ2IN88US=> MY\TRW5ITVJ?
HT M3K5fWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXrJR|UxRTBwMkmzPEDPxE1? NF7Nb5dUSU6JRWK=
A3-KAW MkTuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFjOcIhKSzVyPUCuNlk4PCEQvF2= NYjxcJRSW0GQR1XS
LB2241-RCC NIHHXXdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYjJR|UxRTBwM{CxPVgh|ryP M1T3[3NCVkeHUh?=
BB65-RCC M3PYUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NE\Pd4JKSzVyPUCuN|A5ODVizszN MXjTRW5ITVJ?
G-401 MkW1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoDETWM2OD1yLkOwPVg3KM7:TR?= MoPzV2FPT0WU
TYK-nu Ml\FS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWPJR|UxRTBwM{G4OlQh|ryP MlzzV2FPT0WU
NCI-SNU-1 M{nNR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWLzbmNFUUN3ME2wMlMzOzNzIN88US=> M{jVXHNCVkeHUh?=
CHL-1 MlrPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUnJR|UxRTBwM{K3NFMh|ryP NVPQeIxnW0GQR1XS
P30-OHK NHfoOWpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3LhTGlEPTB;MD6zNlcxPSEQvF2= NWrvbGdZW0GQR1XS
PA-1 M3zZV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1HKT2lEPTB;MD6zN|YyQSEQvF2= NHTFN|lUSU6JRWK=
LB1047-RCC MmDpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4LNU2lEPTB;MD6zOFA1PyEQvF2= MWTTRW5ITVJ?
EM-2 MnfnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlLsTWM2OD1yLkO0NVg5KM7:TR?= M3XqenNCVkeHUh?=
VA-ES-BJ M2[0Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHu3cXBKSzVyPUCuN|QzPjhizszN NV;hWHZ6W0GQR1XS
NCI-H520 MnjwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUjqN2QyUUN3ME2wMlM1QDN2IN88US=> MkDpV2FPT0WU
ECC10 M2jTfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUPHUlRbUUN3ME2wMlM2ODN3IN88US=> MXzTRW5ITVJ?
LNCaP-Clone-FGC M3jHcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlTmTWM2OD1yLkO1N|E1KM7:TR?= MXjTRW5ITVJ?
ES8 Mne2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGDRVpRKSzVyPUCuN|Y2PTNizszN MY\TRW5ITVJ?
HuO-3N1 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVTJR|UxRTBwM{izO|kh|ryP NWXqVWt4W0GQR1XS
L-363 MlXDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXrmc5d2UUN3ME2wMlM6ODJ4IN88US=> M3fNe3NCVkeHUh?=
SK-LU-1 NEPrZZBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3nScWlEPTB;MD6zPVIxQCEQvF2= Mk\2V2FPT0WU
MCF7 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVzJR|UxRTBwNEC1N{DPxE1? MVHTRW5ITVJ?
HuO9 NIHWUY5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUPJR|UxRTBwNEC2Olch|ryP NF\MRZNUSU6JRWK=
BHT-101 M4nGOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnfYTWM2OD1yLkSwO|g4KM7:TR?= MUXTRW5ITVJ?
MZ2-MEL NUfNcIRUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoXqTWM2OD1yLkSwPFgzKM7:TR?= MXnTRW5ITVJ?
NOS-1 M4jZR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MofzTWM2OD1yLkSxNFc1KM7:TR?= M1SzNnNCVkeHUh?=
NEC8 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEPzOWhKSzVyPUCuOFE3QTVizszN M{foZ3NCVkeHUh?=
COR-L88 Mny4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4noZ2lEPTB;MD60NlI3PyEQvF2= MmLzV2FPT0WU
MFM-223 MnLyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFS3b25KSzVyPUCuOFI3OTdizszN M3vQdXNCVkeHUh?=
HSC-3 NHXucWhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHzJeWhKSzVyPUCuOFI6ODhizszN NXHNTXo{W0GQR1XS
OVCAR-5 NU\hd|dlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1Xu[2lEPTB;MD60Nlk{OyEQvF2= NELIXYJUSU6JRWK=
MFE-280 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYHRZmxNUUN3ME2wMlQ{ODN{IN88US=> NIfIbpBUSU6JRWK=
KP-N-YS MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1vjZ2lEPTB;MD60N|A4KM7:TR?= M4HwVXNCVkeHUh?=
EPLC-272H NHrteI9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmXnTWM2OD1yLkSzNFc4KM7:TR?= NXS5T2pZW0GQR1XS
KE-37 NX\2OpNvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGrPVIFKSzVyPUCuOFMzPDdizszN NGT6UpBUSU6JRWK=
HuH-7 M3;KTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFfYRlJKSzVyPUCuOFM{PzJizszN NEXTZXJUSU6JRWK=
CHP-212 NI[2bWVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmfWTWM2OD1yLkSzOlk4KM7:TR?= NU\NS2V7W0GQR1XS
SK-MES-1 NHPIXmZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXrJR|UxRTBwNEO4OFgh|ryP NUfFdphjW0GQR1XS
SJSA-1 NFfiN3FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn;VTWM2OD1yLkS0OVU1KM7:TR?= NXj4S2VJW0GQR1XS
QIMR-WIL MnXDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NY\KUXdjUUN3ME2wMlQ2OTVizszN NGX3OHVUSU6JRWK=
ES4 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF62VG1KSzVyPUCuOFU{OzZizszN NXfKNZJ1W0GQR1XS
TI-73 NXi3RZZFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXzJR|UxRTBwNEW2PFYh|ryP NV7FcplnW0GQR1XS
COLO-684 MlW2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHTLR3BKSzVyPUCuOFU4OjZizszN M1PyPXNCVkeHUh?=
GCIY MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1PKeGlEPTB;MD60OVc2PCEQvF2= M{e4XXNCVkeHUh?=
JVM-3 M{Txd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXnJR|UxRTBwNE[yPVIh|ryP MWXTRW5ITVJ?
MPP-89 MlnGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4OzbmlEPTB;MD60Olk1PyEQvF2= MYfTRW5ITVJ?
M14 NITGfGNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH7qV5VKSzVyPUCuOFcyPyEQvF2= NUHWcGwyW0GQR1XS
SW962 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmfETWM2OD1yLkS3NVkh|ryP NFj2SlZUSU6JRWK=
VM-CUB-1 NFnHTWFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mkj1TWM2OD1yLkS3Nlg3KM7:TR?= NXHIdHc3W0GQR1XS
A101D NWfCW4ZzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4\YcGlEPTB;MD60PFk3QCEQvF2= Ml[5V2FPT0WU
DB MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUDJR|UxRTBwNUCwNlEh|ryP MoPHV2FPT0WU
KNS-62 NHS3PY5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVzlTZRwUUN3ME2wMlUxPTZ6IN88US=> MlPHV2FPT0WU
NCI-H460 M1X4dWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlXqTWM2OD1yLkWwOVk4KM7:TR?= MUfTRW5ITVJ?
MDA-MB-361 MnHaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYnJR|UxRTBwNUC4O{DPxE1? MkTZV2FPT0WU
DOK NYribZdET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGXPXHFKSzVyPUCuOVE{OzNizszN NFH0SFJUSU6JRWK=
CAL-39 M3HTW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX;JR|UxRTBwNUKyOlgh|ryP MUnTRW5ITVJ?
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LB2518-MEL NV;ZToFWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NY\ySnBqUUN3ME2yMlY2PDB6IN88US=> MWTTRW5ITVJ?
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OVCAR-3 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkjqTWM2OD1|LkCyNVE2KM7:TR?= M3XkZXNCVkeHUh?=
KLE MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mkm0TWM2OD1|LkC2PFEzKM7:TR?= NEHTVFZUSU6JRWK=
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SCC-9 MlW3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4fncmlEPTB;Mz6xNVM3PiEQvF2= M4fuOHNCVkeHUh?=
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LCLC-103H MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoPvTWM2OD1|LkKzPFk1KM7:TR?= MlrHV2FPT0WU
U-266 NHTzUIFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4nFemlEPTB;Mz6yO|Y{OyEQvF2= MVjTRW5ITVJ?
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UACC-257 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFP5eWxKSzVyPUOuOFY1OzFizszN MU\TRW5ITVJ?
LB996-RCC NELCTnFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmjnTWM2OD1|LkS2OVAzKM7:TR?= NF7SbGpUSU6JRWK=
BT-549 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MojNTWM2OD1|LkWwNlA6KM7:TR?= MWHTRW5ITVJ?
NCI-H526 Mn7OS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4fV[GlEPTB;Mz61OFE2QCEQvF2= NInvSpRUSU6JRWK=
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SW626 NGH4cJpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYraSWp4UUN3ME2zMlcyOTJ6IN88US=> NYLWSHJSW0GQR1XS
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IGR-1 NXLKXHpNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUPnPWpnUUN3ME2zMlc1OjR6IN88US=> MXfTRW5ITVJ?
NCI-H2291 NF7UN|dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkDJTWM2OD1|Lke1N|M3KM7:TR?= M4LubXNCVkeHUh?=
NCI-H441 NIPLb|JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkDSTWM2OD1|LkiyOFM{KM7:TR?= Mom3V2FPT0WU
D-247MG MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWLJR|UxRTNwOE[wN|Eh|ryP M2Xm[XNCVkeHUh?=
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HT55 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUjJR|UxRTNwOUG3O|Mh|ryP MVzTRW5ITVJ?
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RERF-LC-MS NVvleJpUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlrlTWM2OD1|Lkm2OVI2KM7:TR?= MVLTRW5ITVJ?
SF268 M3Xj[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlzlTWM2OD12LkGyPFgyKM7:TR?= Mn\SV2FPT0WU
RMG-I M{jFeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{HaZ2lEPTB;ND6yNVQ2PCEQvF2= M1jGbnNCVkeHUh?=
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S-117 M1;td2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MonGTWM2OD12LkKyNFI1KM7:TR?= M{HjTnNCVkeHUh?=
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J82 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4jwR2lEPTB;ND6zN|Q1OSEQvF2= MVvTRW5ITVJ?
CAL-72 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXPJR|UxRTRwM{W0O|ch|ryP NYPNdJk1W0GQR1XS
GP5d MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXPJR|UxRTRwNEGyPVgh|ryP M3X1bXNCVkeHUh?=
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DK-MG NXvWdFVjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{PCZmlEPTB;ND63PFEyOiEQvF2= MoHyV2FPT0WU
HCC1937 MmfCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWnESmFwUUN3ME20Mlg2OTJ3IN88US=> MXHTRW5ITVJ?
OAW-28 NHvhT4dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGXrenVKSzVyPUSuPVI1OzVizszN NFTT[VhUSU6JRWK=
MEG-01 MnTtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX21NIxmUUN3ME21MlA5OzJ6IN88US=> NYX2XolxW0GQR1XS
GI-1 M4fx[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWjQUllTUUN3ME21MlI{PDh4IN88US=> NVjadYluW0GQR1XS
FADU NEHaemdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVjJR|UxRTVwMke4N|ch|ryP M1fSS3NCVkeHUh?=
CAPAN-1 MkDVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4P3TWlEPTB;NT6zPVQ{OiEQvF2= MX7TRW5ITVJ?
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DBTRG-05MG MlPBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXzJR|UxRTVwNUC1NkDPxE1? MXvTRW5ITVJ?
ONS-76 NIfSOpdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3fhPWlEPTB;NT61OVgxOSEQvF2= M{nzbXNCVkeHUh?=
Ca-Ski MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3XiPWlEPTB;NT62NlU3PiEQvF2= MX\TRW5ITVJ?
COLO-741 NEfIZppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2rBRmlEPTB;NT63NVk6PSEQvF2= NY[0eWhtW0GQR1XS
HT-29 NUDxeI8{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUTJR|UxRTVwOEC0O|Uh|ryP MmnNV2FPT0WU
HCC1187 M{Czc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2D3UWlEPTB;NT64NVAxQCEQvF2= MYrTRW5ITVJ?
Capan-2 M{j1Zmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2LReWlEPTB;NT65OVk6PCEQvF2= M{D0SnNCVkeHUh?=
NCI-H630 MlTWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYDJR|UxRTZwMUG0OFEh|ryP NHjZfWJUSU6JRWK=
COLO-678 M2rxeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHuzNHVKSzVyPU[uNVUxQTJizszN MnnTV2FPT0WU
D-502MG NWT2VHVoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHXuWIxKSzVyPU[uNVc{OzZizszN NYXvbXJDW0GQR1XS
NB17 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWHJ[lhXUUN3ME22MlI1QTR|IN88US=> NFjsZnVUSU6JRWK=
SK-MEL-24 NE\HVVdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYf1V4l2UUN3ME22MlI3PDlizszN MofEV2FPT0WU
NCI-SNU-5 NUK4U2tKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWHqVotOUUN3ME22MlI4OTF6IN88US=> MXPTRW5ITVJ?
D-392MG MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlXkTWM2OD14LkOzN|k6KM7:TR?= MnLJV2FPT0WU
A498 MlK4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWLLbFN3UUN3ME22MlQxOzB{IN88US=> NFzHbIhUSU6JRWK=
OCI-AML2 M1[5O2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3fyS2lEPTB;Nj60OVQ1PSEQvF2= MXnTRW5ITVJ?
HCC2157 Mnv3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXHJR|UxRTZwNEW2NVYh|ryP MXTTRW5ITVJ?
C3A M1LrOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXfrd|J[UUN3ME22MlUzODB2IN88US=> MljQV2FPT0WU
NCI-H1650 Mn;vS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUj4fHU1UUN3ME22MlcxPTJ{IN88US=> NFrLPI5USU6JRWK=
DU-145 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWXafXU1UUN3ME22Mlc4ODNizszN MWPTRW5ITVJ?
COLO-829 MoTkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGTwd2xKSzVyPU[uO|c5PzNizszN MkO0V2FPT0WU
LS-1034 MkXFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{PBdWlEPTB;Nz6wOFQyOiEQvF2= MmC4V2FPT0WU
HCC1954 M1TwXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV3qcpUyUUN3ME23MlQ5OTN2IN88US=> Mle1V2FPT0WU
KURAMOCHI MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mo\wTWM2OD15LkS4OVM{KM7:TR?= NEDuVGlUSU6JRWK=
Mo-T NIOwRZhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mnj6TWM2OD15LkWxOFk5KM7:TR?= MnfSV2FPT0WU
OE19 NGrHbGdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUjJR|UxRTdwNkW1NFMh|ryP Mkj4V2FPT0WU
COR-L105 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXPJR|UxRTdwN{G0NlEh|ryP MkTBV2FPT0WU
NCI-H2342 MkjXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2[2SWlEPTB;Nz63NVUzOSEQvF2= MUfTRW5ITVJ?
U-87-MG MoWwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHno[W9KSzVyPUiuNFM{OzRizszN M4TucXNCVkeHUh?=
MKN28 NHe5N2RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVH6S2xvUUN3ME24MlEyPTF3IN88US=> Mn\XV2FPT0WU
HT-1197 Mn;xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXzJR|UxRThwMkW5NFch|ryP Mon5V2FPT0WU
ChaGo-K-1 M{fQPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1G4OmlEPTB;OD6zOFI1PSEQvF2= NXHJbZR7W0GQR1XS
NCI-H226 NVTjNWx5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXTJR|UxRThwNESzN|Eh|ryP MWDTRW5ITVJ?
NCI-H2029 Mle3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV20S|hpUUN3ME25MlA3PzV7IN88US=> MXXTRW5ITVJ?
TE-11 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXLJR|UxRTlwNEW1PFgh|ryP MYHTRW5ITVJ?
ZR-75-30 NFjWPYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEXCW5VKSzVyPUmuPVI3OzRizszN M3PkN3NCVkeHUh?=
SW837 M1X3dWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYrJR|UxRTFyLkKyPVMh|ryP NF3JbpRUSU6JRWK=
NCI-H1651 NGPYNJBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXfJR|UxRTFyLkKzNlYh|ryP NV7RcWpHW0GQR1XS
NCI-H727 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXTNc4RpUUN3ME2xNE41PDl3IN88US=> Mn6wV2FPT0WU
NCI-H358 NHv3W5ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUXJR|UxRTFyLkizOlUh|ryP NGHUdWNUSU6JRWK=
NCI-H2087 Mk\uS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWK0cGNZUUN3ME2xNU4xPDN2IN88US=> MW\TRW5ITVJ?
FTC-133 MmXBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVnJR|UxRTFzLkSwOVEh|ryP MkDLV2FPT0WU
LAN-6 M2S5emdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4jhOWlEPTB;MUKuNVI1KM7:TR?= MVTTRW5ITVJ?
NCI-H1838 NFn5WWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnnXTWM2OD1zMj61NVUyKM7:TR?= MnfYV2FPT0WU
Calu-6 MkP5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHntXXhKSzVyPUG0MlExOjFizszN NYfONXVGW0GQR1XS
HCC70 M{TRW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVPMNYtDUUN3ME2xOE41PTFizszN NWnVUW5TW0GQR1XS
KARPAS-299 M{Hocmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3;2T2lEPTB;MUWuNlE2QSEQvF2= M{DhXHNCVkeHUh?=
CAL-27 M{HXXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIn1eHFKSzVyPUG1MlM5PDNizszN M2fXW3NCVkeHUh?=
NCI-H82 MoDYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4\MXWlEPTB;MUWuOVMzKM7:TR?= NXLV[Hl6W0GQR1XS
no-10 NHvqWllIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUXJR|UxRTF3LkmzPEDPxE1? MlL0V2FPT0WU
U251 M1LSN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFrjWYZKSzVyPUG2MlM3PzVizszN NWT2Z5lvW0GQR1XS
TE-9 NF7mVpBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn:wTWM2OD1zNz61NVY3KM7:TR?= MnnNV2FPT0WU
TE-10 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXryRWp5UUN3ME2xPU4xPjB5IN88US=> MYfTRW5ITVJ?
COLO-824 Mn\vS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH7vW3dKSzVyPUKwMlkzPzZizszN M1e4UHNCVkeHUh?=
HuP-T3 NUTlSpVUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWXoVYg2UUN3ME2yNU4xPzR6IN88US=> M3zLNXNCVkeHUh?=
KALS-1 MnfuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUT3dGhRUUN3ME2yNU4yPzVizszN MkH5V2FPT0WU
L-428 Ml3tS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlrSTWM2OD1{MT60NFE3KM7:TR?= MnrEV2FPT0WU
JAR Mk[0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV7YSJRRUUN3ME2yNU42ODJizszN M1nSbnNCVkeHUh?=
DoTc2-4510 M3;2bWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NW\oUolVUUN3ME2yN{45PDd3IN88US=> NYmyRoVXW0GQR1XS
ABC-1 NHf2b3NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWLJR|UxRTJ4Lke5OVch|ryP MlP1V2FPT0WU
MHH-NB-11 NEXMPHZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFWwR5JKSzVyPUOzMlM1PjRizszN MYDTRW5ITVJ?
SW1463 NHrnSJJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH;lS4hKSzVyPUOzMlk5OyEQvF2= NXP1e2VlW0GQR1XS
SW1417 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWXJR|UxRTN5LkC5OVIh|ryP NIKx[WJUSU6JRWK=
NB12 M1LMTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4jCXmlEPTB;M{muOFY2PSEQvF2= M1XuSHNCVkeHUh?=
NY NVvFT|VwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHPyeWVKSzVyPUSxMlMzODFizszN MYHTRW5ITVJ?
H1048 MYnBdI9xfG:|aYOgRZN{[Xl? MYC1NFAhdk1? MX63NkBp MoPncohidmOnczDBRnQuOjZ|4pETbY5lfWOnZDDhdI9xfG:|aYO= M3;WUFI2PzN5NUSy
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H196 NU[5VmhuSXCxcITvd4l{KEG|c3H5 M33GNVUxOCCwTR?= MXq3NkBp MX;ubIFv[2W|IFHCWE0zPjQkgKPpcoR2[2WmIHHwc5B1d3Orcx?= Ml7iNlU4Ozd3NEK=
H446 MWXBdI9xfG:|aYOgRZN{[Xl? NXfMNZJiPTByIH7N NYnTfFhFPzJiaB?= M{TqdI5p[W6lZYOgRWJVNTJ4M,MAl4lv\HWlZXSgZZBweHSxc3nz MWCyOVc{PzV2Mh?=
TFK-1 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1P0UlczKGh? MWTEUXNQ NFnoPFVKSzVyPUS3JI5O NH3pe5AzPTF3MkK0OC=>
EGI-1 NFThSJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mo[zO|IhcA>? MlHGSG1UVw>? MUDJR|UxRTV|IH7N Mm\uNlUyPTJ{NES=
OCI/AML3 NIXNOm1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4exSVAvOiEQvF2= MVe0PEBp MU\lcohidmOnczDj[YxtKGe{b4f0bEBqdmirYnn0bY9vKGmwZIXj[YQh[nlic3XseY1mfGmwaXK= M3jCWVI1PzN7M{mz
U937 M4Wwfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGm0VogxNjJizszN NY\SXVJ4PDhiaB?= M2jRSIVvcGGwY3XzJINmdGxiZ4Lve5RpKGmwaHnibZRqd25iaX7keYNm\CCkeTDz[Yx2dWW2aX7pZi=> Ml;JNlQ4Ozl|OUO=
MOLM13 MlPHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoO0NE4zKM7:TR?= M4L3clQ5KGh? NHLG[VlmdmijbnPld{Bk\WyuIHfyc5d1cCCrbnjpZol1cW:wIHnu[JVk\WRiYomgd4VtfW2ndHnubYI> NHK2dpUzPDd|OUO5Ny=>
MCF7 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWmyNEBvVQ>? NYD0ZpVzPzJiaB?= Mm\jSmJU M2HSUJJm\HWlZYOgeIhmKFCHTGCxMY1m\GmjdHXkJIlv[3KnYYPlJIlvKGOnbHygeoli[mmuaYT5 NULiNpZkOjR4OEiwOFY>
TamR M4rKfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NID1WVBKSzVyPUG45qCKdk1? MnzHNlQ1PTdyNkm=
MCF7-X  NUTvc29tT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlPFTWM2OD1{NDDuUS=> MnTvNlQ1PTdyNkm=
T47D-tamR  M1;Hcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWXJR|UxRTF7IH7N MV6yOFQ2PzB4OR?=
293T  NVG1cGxmTnWwY4Tpc44hSXO|YYm= M{TWT|IxKG6P MYezJIg> MlnD[Il{enWydIOgRYt1NW2nZHnheIVlKFSLRj3JRUB{fGGkaXzpeJktKHS{YX7zcI9k[XSrb36sJIFv\CCjY4Tpeol1gQ>? NV\zVopuOjR|NkO0OFk>
SW620 NF3JTnlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWKwMlAxOS1zMN88US=> NVLoVYhMOjRiaB?= MnzKbY5pcWKrdIOgeIhmKGOnbHygdJJwdGmoZYLheIlwdiCjczD0bIUh[2:wY3XueJJifGmxbjDybZNmew>? MmTuNlQ{PjN2NEm=
Huh7  MlKwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2HWblUuOTByIH7N NV\hZpJqOC1{NDDo M3LYfWROW09? NVz4XFh5cW6lcnXhd4V{KGOnbHyg[IVifGhiaX6gZUBkd26lZX70doF1cW:wLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{ M4exblI1Ojl5M{Cw
Hep3B  NIXGN4hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVn0cYtWPS1zMECgcm0> NEX4bYoxNTJ2IHi= NVK1dFFvTE2VTx?= NV;kOlYxcW6lcnXhd4V{KGOnbHyg[IVifGhiaX6gZUBkd26lZX70doF1cW:wLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{ Mo\XNlQzQTd|MEC=
Huh7  NWHnOXZRTnWwY4Tpc44hSXO|YYm= MkjaNVAxKG6P NETIOooyOiCq MYTEUXNQ MWTpcoR2[2W|IHH1eI9xcGGpedMg MmnQNlQzQTd|MEC=
U87-MG  M4\4emdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NID2S5g4OiCq NISzNJNIUTVy4pEJQgKBkTd7IH7NxsA> MYmyOFI1QTZ2MB?=
 L3.6pl MmTCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1L2clEh|ryP MYq3NkBp M1zLR4lvcGmkaYTzJJRp\SClZXzsJIdzd3e2aDDjc41jcW6nZDD3bZRpKEKPUz23O|c3ODd? M2jr[|I1OjN|M{m5
TE671 MoLSRZBweHSxc3nzJGF{e2G7 NYeyfolXOSEQvF2= MXq0PEBp NFLvNFRqdmO{ZXHz[ZMh[2WubDDhdI9xfG:|aYOgbY5lfWOnZDDifUBCSlRvN{O3 NV3xRWh5OjRzM{OyNVg>
RD M1\ZZWFxd3C2b4Ppd{BCe3OjeR?= MUixJO69VQ>? NIXVZWE1QCCq M3jpVolv[3KnYYPld{Bk\WyuIHHwc5B1d3OrczDpcoR2[2WmIHL5JGFDXC15M{e= MlXpNlQyOzN{MUi=
RMS13 M1vRR2Fxd3C2b4Ppd{BCe3OjeR?= NXzqc|JDOSEQvF2= NVzscmRxPDhiaB?= M3PKNolv[3KnYYPld{Bk\WyuIHHwc5B1d3OrczDpcoR2[2WmIHL5JGFDXC15M{e= MVSyOFE{OzJzOB?=
RH30 M4DXOWFxd3C2b4Ppd{BCe3OjeR?= Mk\qNUDPxE1? NVi4OoQzPDhiaB?= NV7mT2RtcW6lcnXhd4V{KGOnbHygZZBweHSxc3nzJIlv\HWlZXSgZpkhSUKWLUezOy=> NXz2RoxQOjRzM{OyNVg>
Hep-2 MoXsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWTjN5pWOC5yMT2xJO69VQ>? M{fXZ|EzNTl4IHi= NX;LfmF6cW6lcnXhd4V{KGOnbHyg[IVifGhiaX6gZUBkd26lZX70doF1cW:wLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{ M{\SdVI1OTB|N{S5
SCC-9 Mn\WS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHnrPVAxNjBzLUGg{txO M4fnWFEzNTl4IHi= M163O4lv[3KnYYPld{Bk\WyuIHTlZZRpKGmwIHGgZ49v[2WwdILheIlwdi1iYX7kJJRqdWVvZHXw[Y5l\W62IH3hco5meg>? MnTYNlQyODN5NEm=
Hep-2 NVXJNFd{TnWwY4Tpc44hSXO|YYm= Mkn4NE4yNzBwNTFOwG0> MY[yOEBp MoXYZoxw[2u|IH3UU3JEOSCjbnSgcXRQWkN{IHHjeIl3[XSrb36= MWGyOFExOzd2OR?=
SCC-9 MorWSpVv[3Srb36gRZN{[Xl? NHjyb|YxNjFxMD61JO69VQ>? M2nvSVI1KGh? M2H3SYJtd2OtczDtWG9TSzFiYX7kJI1VV1KFMjDhZ5RqfmG2aX;u NFOze2EzPDFyM{e0PS=>
PANC-1 NFftSpNCeG:ydH;zbZMhSXO|YYm= NELifng2ODBibl2= M3jQfFQ5KGh? NWjjdHh{e2Wwc3n0bZpmeyCyYX7jdoVifGmlIHPhcoNmeiClZXzsd{B1dyC{YXTpc5Rp\XKjcIm= NWPGR|NKOjN6OE[yPVQ>
RMG2 Mn7tRZBweHSxc3nzJGF{e2G7 M4nEPFEuOTByMDDuUS=> NH3nNZM4OiCq NHTZR3ZHSlN? MYHpcohq[mm2czD0bIUh[2WubDD2bYFjcWyrdImgZ49v[2WwdILheIlwdiCmZYDlcoRmdnSueR?= NVHoUmVOOjN4MUW2N|E>
HAC2 NYTnT3RqSXCxcITvd4l{KEG|c3H5 M2n5Z|EuOTByMDDuUS=> NYHKcG1TPzJiaB?= NEe2dIxHSlN? NX61eplxcW6qaXLpeJMhfGinIHPlcIwhfmmjYnnsbZR6KGOxbnPlcpRz[XSrb36g[IVx\W6mZX70cJk> NX;LRXg{OjN4MUW2N|E>
HT-29 MlS4RZBweHSxc3nzJGF{e2G7 MUCxJO69VQ>? M4m2V|czKGh? NYXke5pr[XS2ZX71ZZRmeyB3LV\VMYlv\HWlZXSgZ4VtdCCmZXH0bC=> MnTENlE6PDlzMkG=
DLD1 NYq2[5BFSXCxcITvd4l{KEG|c3H5 MmnrNUDPxE1? MYq3NkBp NXu4Tmh{[XS2ZX71ZZRmeyB3LV\VMYlv\HWlZXSgZ4VtdCCmZXH0bC=> NFi2eXMzOTl2OUGyNS=>
HT-29 MXvGeY5kfGmxbjDBd5NigQ>? MUOxJO69VQ>? NXexWphjPzJiaB?= NWPid4NGcW6mdXPld{BifXSxcHjh[5khfG9iY3;u[oVzKGO7dH;wdo91\WO2aX;u NY\hboYzOjF7NEmxNlE>
DLD1 MXPGeY5kfGmxbjDBd5NigQ>? Mn\INUDPxE1? MlyxO|IhcA>? M1T3eIlv\HWlZYOgZZV1d3CqYXf5JJRwKGOxbn\ldkBkgXSxcILveIVkfGmxbh?= NHvrOYozOTl2OUGyNS=>
Rh41 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{\UT2lEPTB;M{CuO{BvVQ>? Mk\mNlE{Ozd4N{m=
Rh18 MkHGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFv0cYNKSzVyPUSxMlQhdk1? MkG2NlE{Ozd4N{m=
Rh30 M4DGOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{W3XmlEPTB;MUSgcm0> NXHCTllxOjF|M{e2O|k>
BT-12 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVzjcoltUUN3ME2yOE44KG6P M4rBRlIyOzN5Nke5
CHLA-266 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWrJR|UxRTFwMTDuUS=> MVOyNVM{PzZ5OR?=
TC-71 NVPTW2ZjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXrpNWNlUUN3ME20Ok42KG6P NUHnOJZuOjF|M{e2O|k>
CHLA-9 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYjJR|UxRTF3LkOgcm0> M3XudVIyOzN5Nke5
CHLA-10 MlHPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWXwPYtQUUN3ME2zMlkhdk1? NWq2UnJPOjF|M{e2O|k>
CHLA-258 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV;0XZdmUUN3ME2xNE41KG6P M1HiSlIyOzN5Nke5
GBM2 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3\sbGlEPTB;NECuO{BvVQ>? MWSyNVM{PzZ5OR?=
NB-1643 NFHDcoNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUfJR|UxRTN|Lkigcm0> NX6xZ3FsOjF|M{e2O|k>
NB-EBc1 MoDUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NI\1[olKSzVyPUK3MlEhdk1? M3PqeFIyOzN5Nke5
CHLA-90 NXTYSINpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEHSdXBKSzVyPUO0MlUhdk1? NILS[3kzOTN|N{[3PS=>
CHLA-136 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1rxW2lEPTB;MU[yMlYhdk1? MWWyNVM{PzZ5OR?=
NALM-6 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXvrR5FvUUN3ME2zNk46KG6P MkPqNlE{Ozd4N{m=
COG-LL-317 NWXFdmR1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2XGS2lEPTB;MT6zJI5O NH;Pb3kzOTN|N{[3PS=>
RS4;11 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3PSVGlEPTB;M{Wgcm0> MmPGNlE{Ozd4N{m=
MOLT-4 M2W1c2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3WyfGlEPTB;OD62JI5O NHntd20zOTN|N{[3PS=>
CCRF-CEM Mmq1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWPJbVZLUUN3ME20MlEhdk1? M336UVIyOzN5Nke5
Kasumi-1 NHOyPGpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVrtV4dxUUN3ME2xMlQhdk1? NIW1UI8zOTN|N{[3PS=>
Karpas-299 NXrzXJVDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUnJR|UxRTJzLkegcm0> MXGyNVM{PzZ5OR?=
Ramos-RA1 NE\NNlNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIHVOnlKSzVyPUiuNkBvVQ>? MV6yNVM{PzZ5OR?=
H838 M4rMO2Z2dmO2aX;uJGF{e2G7 MoHtNUDPxE1? MVO0PEBp MmDObY5lfWOnczDj[YxtKGG3dH;wbIFogQ>? NYnXVIxYOjB|NkSxNVM>
H838 NUjpNXdVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoHTNE0{KM7:TR?= MUG3NkBp NG[3VndqdmS3Y3XzJIdzd3e2aDDpcohq[mm2aX;uJIFv\CCjdYTvdIhi\3l? MUKyNFAzQDh3NB?=
A549  MkHmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUHJOVlFOC1|IN88US=> NW[3Wo5oPzJiaB?= MmOxbY5lfWOnczDndo94fGhiaX7obYJqfGmxbjDhcoQh[XW2b4DoZYd6 NHfifoYzODB{OEi1OC=>

... Click to View More Cell Line Experimental Data

In vivo AZD8055 inhibits the pS6 and pAkt in U87MG and A549 xenografts at 2.5/10 mg/kg, which leads to tumor growth inhibition. AZD8055 shows significant antitumor activity in many xenografts, including U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA at a dose of 10/20 mg/kg. [1] AZD8055 induces ~40% reduction in tumour volume, accompanied by ablation of phosphorylation of Akt, S6K and SGK protein kinases. Administration of AZD8055 (5mg/kg, Bid) and SAHA (100 mg/kg/d) results in complete tumor growth inhibition in PTEN+/−LKB1+/hypo xenografts without side effects on mice by inhibition of mTORC1 and mTORC2 signaling. [4]

Protocol

Kinase Assay:[1]
+ Expand

Cell-based determinations of mTOR inhibition:

A high-throughput screening cell-based assay is developed using MDA-MB-468 cells to detect mTORC1 and mTORC2 activity. Cells are exposed for 2 hours to increasing concentrations of AZD8055. At the end of the incubation period, cells are fixed, washed, and probed with antibodies against S473 pAkt or against S235/236 phosphorylated S6 (pS6). Levels of phosphorylation are assessed using an Acumen laser scanning cytometer.
Cell Research:[1]
+ Expand
  • Cell lines: U87MG, A549 and H838 cells
  • Concentrations: 1 nM - 1 μM
  • Incubation Time: 72 to 96 hours
  • Method: Cells are exposed to AZD8055 for 72 to 96 hours and stained for cell nuclei (0.03 mg/mL Hoechst 33342) and acidic vesicles (1 μg/mL acridine orange). Images are captured at 450 and 536 nm on an ArrayScan II platform, and the percentage of acidic vesicles and the number of cells are quantified. For LC3 assessment, cells are exposed to e64d/pepstatin (10 μg/mL) for 30 to 90 min before incubation with AZD8055. Cells are lysed on ice and analyzed by immunoblotting.
    (Only for Reference)
Animal Research:[1]
+ Expand
  • Animal Models: U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA U87-MG and A549 are established in pathogen-free, female nude mice (nu/nu:Alpk).
  • Formulation: Dissolved in captisol (CyDex, pH 3.0) and diluted to a final captisol concentration of 30% (w/v)
  • Dosages: 2.5-20 mg/kg
  • Administration: Oral gavage once or twice daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 50 mg/mL warmed (107.4 mM)
Ethanol 3 mg/mL (6.44 mM)
Water Insoluble
In vivo Add solvents individually and in order:
4% DMSO+30% PEG 300+ddH2O
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 465.54
Formula

C25H31N5O4

CAS No. 1009298-09-2
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01316809 Completed Glioblastoma Multiforme|Anaplastic Astrocytoma|Anaplastic Oligodendroglioma|Malignant Glioma|Brainstem Glioma National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) March 4, 2011 Phase 1
NCT00999882 Completed Cancer|Advanced Hepatocellular Carcinoma AstraZeneca October 2009 Phase 1
NCT00973076 Completed Cancer|Solid Tumors|Advanced Solid Malignancies AstraZeneca August 2009 Phase 1
NCT00731263 Completed Solid Tumors AstraZeneca July 2008 Phase 1
NCT01194193 Withdrawn Cancer|Advanced Solid Tumours|Lymphomas AstraZeneca null Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID