AZD8055

Catalog No.S1555

AZD8055 Chemical Structure

Molecular Weight(MW): 465.54

AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. Phase 1.

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In DMSO USD 156 In stock
USD 120 In stock
USD 370 In stock
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Cited by 38 Publications

8 Customer Reviews

  • PC3-LN4 cells were treated with GSK690693 (5 uM) alone, or in combination with PP242 (2 uM) or AZD8055 (1 uM) for 24 h, and immunoblotting performed.

    Cancer Res 2013 73(11), 3402-11. AZD8055 purchased from Selleck.

    HUVEC were treated with either RAD001 or AZD8055 before PA treatment. p53, GPX1, p53 acetylation, and subcellular PTEN were analyzed with corresponding antibodies. The blot is a representative of the results of three experiments. Values (mean盨D) are expressed as fold over untreated control. *p<0.05 versus their corresponding control groups in the one-way ANOVA.

    Antioxid Redox Signal 2014 20(9), 1382-95. AZD8055 purchased from Selleck.

  • (A) HCT116 or MDA-MB-468 cells were treated with 500 nM AZD8055 for the indicated times. Cell lysates were immunoblotted with the indicated antibodies. (B) HCT116 or MDA-MB-468 cells were treated with 50 nM rapamycin. 500 nM AZD8055, 1 uM MK2206 or dimethyl sulfoxide (DMSO) as control for 12 h. Cell lysates were immunoblotted with the indicated antibodies.

    Oncotarget 2014 5(15), 6015-27. AZD8055 purchased from Selleck.

    CSCs+24/44/ESA at 5,000 cells per well with stem cell culture media in triplicate in an ultra-low adhesion plate were treated with 5 umol/L BMS-777607, 1 umol/L AZD8055 alone, or in their different combinations. Cells were cultured for 72 hours. Cell viability was determined by the MTS assay. Percentages of polyploid cells were determined by counting 300 cells from two different regions. Results shown here were from one of two experiments with similar results.

    Mol Cancer Ther 2014 13(1), 37-48. AZD8055 purchased from Selleck.

  • mTOR kinase inhibitor AZD8055 activates PI3K accompanied with induction of expression of EGFR, HER2, HER3 and IRS1. Serum-deprived CHO-EGFP-AKT cells were incubated with 50 nM AZD8055 for 24 hr. The EGFP signal was detected using confocal microscopy. The white arrows indicate EGFP-AKT located on cellular membrane.

    Int J Cancer 2014 135(10), 2462-74. AZD8055 purchased from Selleck.

    mTOR, P70S6K1, 4E-BP1 and PCNA activation in the basilar artery of SAH. Top, Western blotting bands of antibodies, bottom, density of the protein band from each group. A, mTOR activation in the basilar artery demonstrated an elevated expression of mTOR on day 7 following SAH in SAH and SAH+DMSO animals. The mTOR inhibitors, Rapamycin and AZD8055 reduced the enhanced expression of mTOR (pb0.05, ANOVA). B. P70S6K1 activation. C. 4E-BP1 activation. D. PCNA activation. Rapamycin and AZD8055 significantly decreased the expression of P70S6K1, 4E-BP1 and PCNA compared with the SAH and SAH+DMSO (pb0.05, respectively; ANOVA). There was no significant difference between the two inhibitors in mTOR, P70S6K1, 4E-BP1 and PCNA expression.

    Exp Neurol 2012 233, 799-806. AZD8055 purchased from Selleck.

  • Induction of TCTP expression through 5-FU or oxaliplatin is inhibited by mTOR kinase inhibitors. HCT116 cells were treated for 48 h with 5-FU or oxaliplatin at the indicated concentrations. Where indicated, AZD 8055 (100 nM) or PP242 (1 μM) were added. TCTP levels were assessed by western blotting; signals were quantified and normalised against α-tubulin as a loading control. As a readout of mTOR activity, phosphorylation of the ribosomal protein S6 at Ser 240/244 was assessed using a phospho-specific antibody. a. Representative western blots demonstrating the alteration of the levels of TCTP protein and of phospho-ribosomal protein S6, respectively, in relation to the drug concentration.

    Cell Commun Signal, 2017, 15(1):9. AZD8055 purchased from Selleck.

    After starved in serum-free medium for 24h , A549 cells incubated with the indicated concentrations of AZD8055 for 3h , followed by 20-minute stimolation of 100ng/ml EGF

     
     

     

    Dr. Zhang of Tianjin Medical University. AZD8055 purchased from Selleck.

Purity & Quality Control

Choose Selective mTOR Inhibitors

Biological Activity

Description AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. Phase 1.
Features First drug to inhibit both types of mTOR protein.
Targets
mTOR (truncated) [1]
(MDA-MB-468 cells)
mTOR (full length) [1]
(MDA-MB-468 cells)
0.13 nM 0.8 nM
In vitro

AZD8055 shows low activity (∼1,000-fold) against all PI3K isoforms (α, β, γ, δ) and other members of the PI3K-like kinase family (ATM and DNA-PK). AZD8055 inhibits the phosphorylation of mTORC1 (p70S6K and 4E-BP1) as well as phosphorylation of the mTORC2 (Akt) and downstream proteins. The rapamycin-resistant T37/46 phosphorylation sites on 4E-BP1 can be fully inhibited by AZD8055, resulting in significant inhibition of cap-dependent translation. AZD8055 potently inhibits proliferation in U87MG, A549 and H838 cells with IC50 of 53, 50 and 20 nM, respectively. AZD8055 also induces autophagy and increased LC3-II levels in H838 and A549 cells. [1] AZD8055 decreases AML blast cell proliferation and cell cycle progression, reduces the clonogenic growth of leukemic progenitors and induces caspase-dependent apoptosis in leukemic cells but not in normal immature CD34+ cells. [2] AZD8055 indicates inhibitory against the pediatric preclinical testing program (PPTP) cell lines with IC50 of 24.7 nM and induces significant differences in EFS distribution. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LU-139 M4P0Z2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVm0fVdEUUN3ME2wMlAzOzNzIN88US=> NGjkXIpUSU6JRWK=
NCI-H720 MnftS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVfJR|UxRTBwMEK5N|gh|ryP NWrBcVRQW0GQR1XS
KARPAS-45 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mor6TWM2OD1yLkCzN|cyKM7:TR?= NFv0b4FUSU6JRWK=
EW-3 MoT5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnntTWM2OD1yLkC0NFU3KM7:TR?= M4LlfXNCVkeHUh?=
SBC-1 NHnEZ4pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2H1NmlEPTB;MD6wOFU5OSEQvF2= M2iyenNCVkeHUh?=
NCI-H209 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MW\JR|UxRTBwMEW0OVMh|ryP NHK3VHNUSU6JRWK=
EoL-1-cell MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYjUOHBIUUN3ME2wMlA3PTZ7IN88US=> Mk\oV2FPT0WU
H9 NEn3PW1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4GzfWlEPTB;MD6wOlY6OiEQvF2= MXrTRW5ITVJ?
AN3-CA NWjNWJJ7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXfJR|UxRTBwMEe5OVEh|ryP NXX5Z3BNW0GQR1XS
HH MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF[zWGZKSzVyPUCuNFgxODlizszN NFLmRWZUSU6JRWK=
KU812 NVuwVoI6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXP3dnZ1UUN3ME2wMlA5ODR|IN88US=> MkjhV2FPT0WU
DU-4475 M3LZWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3HE[GlEPTB;MD6wPFA1PyEQvF2= M4noNnNCVkeHUh?=
CTV-1 NWfmV|Z6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHHDdHNKSzVyPUCuNFg3PDFizszN NUjkUJA1W0GQR1XS
BV-173 MoDnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUP6RYJTUUN3ME2wMlA6ODZzIN88US=> NX3kZ5oxW0GQR1XS
ES5 M17pSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXG4fWs3UUN3ME2wMlA6OzVizszN M3rmWnNCVkeHUh?=
G-402 NH3RbZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXvJR|UxRTBwMEm5PEDPxE1? MUHTRW5ITVJ?
MV-4-11 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NViwTo12UUN3ME2wMlExOzR5IN88US=> NWPFTmJMW0GQR1XS
NB69 MlWyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoK5TWM2OD1yLkGzOUDPxE1? MU\TRW5ITVJ?
EGI-1 NUP2To1YT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUHpTJBKUUN3ME2wMlE{PTNzIN88US=> NWfCbJo{W0GQR1XS
EW-18 NV23WI1TT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXjBNWt{UUN3ME2wMlE1ODd|IN88US=> Ml3ZV2FPT0WU
ME-180 NFXKNIhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3TqXmlEPTB;MD6xOFY5OSEQvF2= Mle3V2FPT0WU
D-283MED NFL6[VBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4XHRWlEPTB;MD6xOFczOyEQvF2= MmrpV2FPT0WU
NB14 NYLEU4Z{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUXRTWx4UUN3ME2wMlE3PDR5IN88US=> NY\xVpFNW0GQR1XS
Ramos-2G6-4C10 MoHpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX\JR|UxRTBwMU[5PFMh|ryP NGPKXHJUSU6JRWK=
NBsusSR MkC3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF;DV2xKSzVyPUCuNVcxOTlizszN M176NHNCVkeHUh?=
LAMA-84 MoTiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWq1PIF5UUN3ME2wMlE5ODN{IN88US=> M4jadXNCVkeHUh?=
NCI-H1581 Ml71S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1\EVmlEPTB;MD6xPFk3PiEQvF2= NFjVXlVUSU6JRWK=
A204 NVXuUndGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NI\xSGZKSzVyPUCuNVg6PyEQvF2= NGjuR45USU6JRWK=
A549 NUG0NpgzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYHJR|UxRTBwMUm1OUDPxE1? MVHTRW5ITVJ?
CAL-51 Mlm4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnyyTWM2OD1yLkG5OlUyKM7:TR?= MUDTRW5ITVJ?
NKM-1 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlzOTWM2OD1yLkKwNFI6KM7:TR?= NXPi[ZdDW0GQR1XS
LoVo M2rrWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHvR[XRKSzVyPUCuNlAzODJizszN M3e1PHNCVkeHUh?=
HAL-01 NEDVWGVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF7IUGZKSzVyPUCuNlAzQTdizszN M3Pr[HNCVkeHUh?=
HCC2218 M1Xhd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYrWU|U{UUN3ME2wMlIxPjh6IN88US=> MkK4V2FPT0WU
BEN NHXWVWNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV;JR|UxRTBwMkGyPVMh|ryP Mn3pV2FPT0WU
LC-2-ad M3;1[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NI\YRpFKSzVyPUCuNlIxQDRizszN NYnLSFg1W0GQR1XS
LXF-289 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFHSRmpKSzVyPUCuNlUxPDFizszN M3PMPHNCVkeHUh?=
HGC-27 NEn0dW9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUfXeVdOUUN3ME2wMlI3PDF2IN88US=> MXfTRW5ITVJ?
LK-2 MkTFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3vyVGlEPTB;MD6yOlgzPCEQvF2= MlT1V2FPT0WU
CTB-1 NEDO[mtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1zVTWlEPTB;MD6yO|IyPSEQvF2= NWm2fHY{W0GQR1XS
697 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M37GbmlEPTB;MD6yO|M3PyEQvF2= NEKzOZFUSU6JRWK=
ES7 MmX1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NW\YdG1{UUN3ME2wMlI4Ozh|IN88US=> NG\y[YxUSU6JRWK=
MG-63 M{\qPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUewSWJvUUN3ME2wMlI4PDB5IN88US=> M{CyZXNCVkeHUh?=
T47D M1nHTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXTJR|UxRTBwMke0O|Uh|ryP NG\UTGRUSU6JRWK=
RPMI-8226 MnzaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1S2cGlEPTB;MD6yPFU5QSEQvF2= NYH6U4FZW0GQR1XS
NCI-H292 NWfJcmlqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MY\JR|UxRTBwMkmxOlQh|ryP NGfmeohUSU6JRWK=
HT NH\QeI9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2TRT2lEPTB;MD6yPVM5KM7:TR?= MXPTRW5ITVJ?
A3-KAW NEiyXm5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHLWPGRKSzVyPUCuNlk4PCEQvF2= NUnmXYxlW0GQR1XS
LB2241-RCC MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoWxTWM2OD1yLkOwNVk5KM7:TR?= MlLmV2FPT0WU
BB65-RCC MoO4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWrJR|UxRTBwM{C4NFUh|ryP MnHaV2FPT0WU
G-401 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX;tVXZoUUN3ME2wMlMxQTh4IN88US=> MkjZV2FPT0WU
TYK-nu MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYTJR|UxRTBwM{G4OlQh|ryP M1myOXNCVkeHUh?=
NCI-SNU-1 Mn3xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV;JR|UxRTBwM{KzN|Eh|ryP MXfTRW5ITVJ?
CHL-1 M{f2OWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlnnTWM2OD1yLkOyO|A{KM7:TR?= NEjHNVFUSU6JRWK=
P30-OHK NEX5NlhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF3TeoFKSzVyPUCuN|I4ODVizszN MmLKV2FPT0WU
PA-1 NEntdJZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVL6[GxSUUN3ME2wMlM{PjF7IN88US=> MXjTRW5ITVJ?
LB1047-RCC MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4HSUGlEPTB;MD6zOFA1PyEQvF2= NHPjbWtUSU6JRWK=
EM-2 NH;RWlFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWnJR|UxRTBwM{SxPFgh|ryP Mk\LV2FPT0WU
VA-ES-BJ NYPVRW9OT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXH5UXJTUUN3ME2wMlM1OjZ6IN88US=> NXHyTYN6W0GQR1XS
NCI-H520 NVe3RXJCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml35TWM2OD1yLkO0PFM1KM7:TR?= NFzLSZZUSU6JRWK=
ECC10 M4nJNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkXBTWM2OD1yLkO1NFM2KM7:TR?= NFu2OlhUSU6JRWK=
LNCaP-Clone-FGC NWHiSWZoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2fLbmlEPTB;MD6zOVMyPCEQvF2= MXrTRW5ITVJ?
ES8 M{jWVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{Dkc2lEPTB;MD6zOlU2OyEQvF2= Moj6V2FPT0WU
HuO-3N1 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXu1c3RWUUN3ME2wMlM5Ozd7IN88US=> NUHITVFFW0GQR1XS
L-363 M1vGT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4jBPGlEPTB;MD6zPVAzPiEQvF2= MkD2V2FPT0WU
SK-LU-1 MnnDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUPJR|UxRTBwM{myNFgh|ryP NVj4bXZbW0GQR1XS
MCF7 NIH6cotIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVHJR|UxRTBwNEC1N{DPxE1? M33UU3NCVkeHUh?=
HuO9 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYjJR|UxRTBwNEC2Olch|ryP NYPabZo2W0GQR1XS
BHT-101 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NE\yd5hKSzVyPUCuOFA4QDdizszN MonYV2FPT0WU
MZ2-MEL MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1yxemlEPTB;MD60NFg5OiEQvF2= MWHTRW5ITVJ?
NOS-1 MlzOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGDHcnFKSzVyPUCuOFExPzRizszN M2W3VnNCVkeHUh?=
NEC8 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M17TSGlEPTB;MD60NVY6PSEQvF2= MkTnV2FPT0WU
COR-L88 M13CO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGXpTFNKSzVyPUCuOFIzPjdizszN MWLTRW5ITVJ?
MFM-223 NFLYbYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFTUO|hKSzVyPUCuOFI3OTdizszN NHvadpVUSU6JRWK=
HSC-3 M1Hqfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MofFTWM2OD1yLkSyPVA5KM7:TR?= NHz4eI5USU6JRWK=
OVCAR-5 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX7QUGNjUUN3ME2wMlQzQTN|IN88US=> NIH0Z4xUSU6JRWK=
MFE-280 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MknOTWM2OD1yLkSzNFMzKM7:TR?= MV3TRW5ITVJ?
KP-N-YS M2jaU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFzM[GNKSzVyPUCuOFMxPyEQvF2= MYfTRW5ITVJ?
EPLC-272H M{jRNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1jUWGlEPTB;MD60N|A4PyEQvF2= NUfDNXpNW0GQR1XS
KE-37 NH22OoZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFfQ[llKSzVyPUCuOFMzPDdizszN NWXVW5pHW0GQR1XS
HuH-7 M{jsc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWexVY1YUUN3ME2wMlQ{Ozd{IN88US=> MkSwV2FPT0WU
CHP-212 M{\GZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUe5[IgzUUN3ME2wMlQ{Pjl5IN88US=> MY\TRW5ITVJ?
SK-MES-1 NHq3Z4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1nobWlEPTB;MD60N|g1QCEQvF2= NGq2cllUSU6JRWK=
SJSA-1 NFL2V4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYrJR|UxRTBwNES1OVQh|ryP NUfWeGJ5W0GQR1XS
QIMR-WIL M3u2TWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4SyfmlEPTB;MD60OVE2KM7:TR?= M3OwPXNCVkeHUh?=
ES4 M3;QOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmriTWM2OD1yLkS1N|M3KM7:TR?= MWnTRW5ITVJ?
TI-73 NHrEXmNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnThTWM2OD1yLkS1Olg3KM7:TR?= NHjJfJZUSU6JRWK=
COLO-684 M{XhUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2rzXGlEPTB;MD60OVczPiEQvF2= MoKwV2FPT0WU
GCIY NXzWXZh3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1TTXmlEPTB;MD60OVc2PCEQvF2= MkGyV2FPT0WU
JVM-3 M4\CUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHXEeo1KSzVyPUCuOFYzQTJizszN Mnz1V2FPT0WU
MPP-89 M2C5Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlHXTWM2OD1yLkS2PVQ4KM7:TR?= MWDTRW5ITVJ?
M14 Ml3US5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVjJR|UxRTBwNEexO{DPxE1? MojaV2FPT0WU
SW962 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NY\1Uph{UUN3ME2wMlQ4OTlizszN MXHTRW5ITVJ?
VM-CUB-1 NXXkVox4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGHsSXZKSzVyPUCuOFczQDZizszN MojyV2FPT0WU
A101D MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUnJR|UxRTBwNEi5Olgh|ryP MVPTRW5ITVJ?
DB NU\PXJlyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEfDdWxKSzVyPUCuOVAxOjFizszN MkLsV2FPT0WU
KNS-62 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVTJR|UxRTBwNUC1Olgh|ryP NF;kT3NUSU6JRWK=
NCI-H460 NVqwZndMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX\JR|UxRTBwNUC1PVch|ryP M4\kRnNCVkeHUh?=
MDA-MB-361 MmXBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUXJR|UxRTBwNUC4O{DPxE1? MmTrV2FPT0WU
DOK MnuyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnvvTWM2OD1yLkWxN|M{KM7:TR?= MnixV2FPT0WU
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OAW-28 MknzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4K5W2lEPTB;ND65NlQ{PSEQvF2= MU\TRW5ITVJ?
MEG-01 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYPJR|UxRTVwMEizNlgh|ryP MUXTRW5ITVJ?
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CAPAN-1 NGnteo5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoL1TWM2OD13LkO5OFMzKM7:TR?= NFn2eHdUSU6JRWK=
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U-87-MG NXTKOYtqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlXNTWM2OD16LkCzN|M1KM7:TR?= MX3TRW5ITVJ?
MKN28 M1vmc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2XoXGlEPTB;OD6xNVUyPSEQvF2= NEnmSlZUSU6JRWK=
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TE-11 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoD1TWM2OD17LkS1OVg5KM7:TR?= NF3FfHBUSU6JRWK=
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FTC-133 MkDoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{fvfmlEPTB;MUGuOFA2OSEQvF2= MV;TRW5ITVJ?
LAN-6 Mke4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlLuTWM2OD1zMj6xNlQh|ryP NXfsboJbW0GQR1XS
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Calu-6 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV\JR|UxRTF2LkGwNlEh|ryP M4HOSHNCVkeHUh?=
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CAL-27 MnHPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MknYTWM2OD1zNT6zPFQ{KM7:TR?= NFvQSnlUSU6JRWK=
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TE-9 MoHDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX;JR|UxRTF5LkWxOlYh|ryP M37CfXNCVkeHUh?=
TE-10 NFizfGZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV;YbGtqUUN3ME2xPU4xPjB5IN88US=> NUKwZYJFW0GQR1XS
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MOLM13 NIfINo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlTFNE4zKM7:TR?= MkXaOFghcA>? NVHET5hT\W6qYX7j[ZMh[2WubDDndo94fGhiaX7obYJqfGmxbjDpcoR2[2WmIHL5JJNmdHWvZYTpcolj NUP2e|ZNOjR5M{mzPVM>
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TamR Mme4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1zkV2lEPTB;MUlihKlvVQ>? M1XweVI1PDV5ME[5
MCF7-X  MoWyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV\JR|UxRTJ2IH7N MnrpNlQ1PTdyNkm=
T47D-tamR  M4\YfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF7JOZFKSzVyPUG5JI5O NETNc|AzPDR3N{C2PS=>
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Huh7  NXnyO4h1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYnvSpdoPS1zMECgcm0> NELzNnoxNTJ2IHi= NHP5NW9FVVOR NFTSSmpqdmO{ZXHz[ZMh[2WubDDk[YF1cCCrbjDhJINwdmOnboTyZZRqd25vIHHu[EB1cW2nLXTldIVv\GWwdDDtZY5v\XJ? NUDGcnloOjR{OUezNFA>
Hep3B  MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2rBdVUuOTByIH7N MYOwMVI1KGh? MUnEUXNQ MX\pcoNz\WG|ZYOgZ4VtdCCmZXH0bEBqdiCjIHPvcoNmdnS{YYTpc44uKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI> MmfuNlQzQTd|MEC=
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U87-MG  NFPCO2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYTwd4dzPzJiaB?= NHvuR4xIUTVy4pEJQgKBkTd7IH7NxsA> MkLiNlQzPDl4NEC=
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TE671 NGDxXphCeG:ydH;zbZMhSXO|YYm= M3LCfFEh|ryP NH7hT|I1QCCq NYfneJllcW6lcnXhd4V{KGOnbHygZZBweHSxc3nzJIlv\HWlZXSgZpkhSUKWLUezOy=> MkDLNlQyOzN{MUi=
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RMS13 NHmxd5dCeG:ydH;zbZMhSXO|YYm= NVq4[IJtOSEQvF2= MWe0PEBp MYfpcoNz\WG|ZYOgZ4VtdCCjcH;weI9{cXNiaX7keYNm\CCkeTDBRnQuPzN5 M13VSVI1OTN|MkG4
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Hep-2 M4XXNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGH2U2IxNjBzLUGg{txO MV2xNk06PiCq NHLHT5VqdmO{ZXHz[ZMh[2WubDDk[YF1cCCrbjDhJINwdmOnboTyZZRqd25vIHHu[EB1cW2nLXTldIVv\GWwdDDtZY5v\XJ? M3;qcVI1OTB|N{S5
SCC-9 M3v3Zmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH3n[3UxNjBzLUGg{txO M4fqV|EzNTl4IHi= NGr6VHlqdmO{ZXHz[ZMh[2WubDDk[YF1cCCrbjDhJINwdmOnboTyZZRqd25vIHHu[EB1cW2nLXTldIVv\GWwdDDtZY5v\XJ? M1P1TFI1OTB|N{S5
Hep-2 MlPwSpVv[3Srb36gRZN{[Xl? MVuwMlEwOC53IN88US=> MorLNlQhcA>? NVLzZXAz[myxY3vzJI1VV1KFMTDhcoQhdVSRUlOyJIFkfGm4YYTpc44> MUGyOFExOzd2OR?=
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PANC-1 MYnBdI9xfG:|aYOgRZN{[Xl? MmLUOVAxKG6P NI\YTYc1QCCq MkfJd4Vve2m2aYrld{Bx[W6lcnXheIlkKGOjbnPldkBk\WyuczD0c{Bz[WSrb4To[ZJieHl? NXL6bYVNOjN6OE[yPVQ>
RMG2 MorrRZBweHSxc3nzJGF{e2G7 M{\GWlEuOTByMDDuUS=> M3nIdFczKGh? MlzQSmJU M{\wOIlvcGmkaYTzJJRp\SClZXzsJJZq[WKrbHn0fUBkd26lZX70doF1cW:wIHTldIVv\GWwdHz5 MlnsNlM3OTV4M{G=
HAC2 NGTH[lRCeG:ydH;zbZMhSXO|YYm= NVXNTIFnOS1zMECwJI5O M2nBRlczKGh? MmnjSmJU M1zaWolvcGmkaYTzJJRp\SClZXzsJJZq[WKrbHn0fUBkd26lZX70doF1cW:wIHTldIVv\GWwdHz5 M1[4VlI{PjF3NkOx
HT-29 NVPzcYJjSXCxcITvd4l{KEG|c3H5 NIKwPHkyKM7:TR?= NFTlWGk4OiCq NEP1cIJifHSnboXheIV{KDVvRmWtbY5lfWOnZDDj[YxtKGSnYYTo M3O0TlIyQTR7MUKx
DLD1 M3XJRWFxd3C2b4Ppd{BCe3OjeR?= NXW4RWJbOSEQvF2= NWXINXdJPzJiaB?= NIXoZWNifHSnboXheIV{KDVvRmWtbY5lfWOnZDDj[YxtKGSnYYTo M120fFIyQTR7MUKx
HT-29 NGm4c4ZHfW6ldHnvckBCe3OjeR?= MX2xJO69VQ>? NGrzRoM4OiCq MUDpcoR2[2W|IHH1eI9xcGGpeTD0c{Bkd26oZYKgZ5l1d3C{b4TlZ5Rqd25? NWOyVYhmOjF7NEmxNlE>
DLD1 MnK4SpVv[3Srb36gRZN{[Xl? NGPOTIsyKM7:TR?= M3[1VlczKGh? M4LpNYlv\HWlZYOgZZV1d3CqYXf5JJRwKGOxbn\ldkBkgXSxcILveIVkfGmxbh?= MmDsNlE6PDlzMkG=
Rh41 NE[1c3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFLoV29KSzVyPUOwMlchdk1? NInNfXkzOTN|N{[3PS=>
Rh18 NWHlO|lMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHXYfopKSzVyPUSxMlQhdk1? Ml3PNlE{Ozd4N{m=
Rh30 NGmyUZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3K2UmlEPTB;MUSgcm0> MYOyNVM{PzZ5OR?=
BT-12 MmjqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnnoTWM2OD1{ND63JI5O NGTPW5UzOTN|N{[3PS=>
CHLA-266 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoW1TWM2OD1zLkGgcm0> M2G1UFIyOzN5Nke5
TC-71 NHjBdppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NE\nS4pKSzVyPUS2MlUhdk1? Ml30NlE{Ozd4N{m=
CHLA-9 M1jtSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnzPTWM2OD1zNT6zJI5O MWKyNVM{PzZ5OR?=
CHLA-10 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWfJR|UxRTNwOTDuUS=> NXzHdoJsOjF|M{e2O|k>
CHLA-258 M3rPVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnOwTWM2OD1zMD60JI5O Mo\uNlE{Ozd4N{m=
GBM2 NUDiXnFJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{DpXmlEPTB;NECuO{BvVQ>? MXiyNVM{PzZ5OR?=
NB-1643 MkfDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFr4[oZKSzVyPUOzMlghdk1? M4faN|IyOzN5Nke5
NB-EBc1 MoTlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV7JR|UxRTJ5LkGgcm0> M3H2XlIyOzN5Nke5
CHLA-90 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3\5TGlEPTB;M{SuOUBvVQ>? MYCyNVM{PzZ5OR?=
CHLA-136 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUi5U|ZbUUN3ME2xOlIvPiCwTR?= NHvTcWwzOTN|N{[3PS=>
NALM-6 M1rvU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVfJR|UxRTN{Lkmgcm0> M4LFPFIyOzN5Nke5
COG-LL-317 M3ziPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWXSZZdPUUN3ME2xMlMhdk1? M3HyZ|IyOzN5Nke5
RS4;11 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{DaS2lEPTB;M{Wgcm0> NIjS[2ozOTN|N{[3PS=>
MOLT-4 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2\ie2lEPTB;OD62JI5O MnvrNlE{Ozd4N{m=
CCRF-CEM MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVXJR|UxRTRwMTDuUS=> MmPjNlE{Ozd4N{m=
Kasumi-1 MlPlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX;JR|UxRTFwNDDuUS=> MYOyNVM{PzZ5OR?=
Karpas-299 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWnJR|UxRTJzLkegcm0> NFPEfHozOTN|N{[3PS=>
Ramos-RA1 M4HVSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWL0XppYUUN3ME24MlIhdk1? MWmyNVM{PzZ5OR?=
H838 NHfNZ2dHfW6ldHnvckBCe3OjeR?= MWCxJO69VQ>? MWO0PEBp M3TCbolv\HWlZYOgZ4VtdCCjdYTvdIhi\3l? NXjoNYs3OjB|NkSxNVM>
H838 NX74[ItRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH7QVpgxNTNizszN MoOyO|IhcA>? M{jZTYlv\HWlZYOg[5Jwf3SqIHnubIljcXSrb36gZY5lKGG3dH;wbIFogQ>? MlqxNlAxOjh6NUS=
A549  NVnEU2dRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXuwMVMh|ryP M3r4NlczKGh? MoHabY5lfWOnczDndo94fGhiaX7obYJqfGmxbjDhcoQh[XW2b4DoZYd6 NXPWb5p2OjByMki4OVQ>

... Click to View More Cell Line Experimental Data

In vivo AZD8055 inhibits the pS6 and pAkt in U87MG and A549 xenografts at 2.5/10 mg/kg, which leads to tumor growth inhibition. AZD8055 shows significant antitumor activity in many xenografts, including U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA at a dose of 10/20 mg/kg. [1] AZD8055 induces ~40% reduction in tumour volume, accompanied by ablation of phosphorylation of Akt, S6K and SGK protein kinases. Administration of AZD8055 (5mg/kg, Bid) and SAHA (100 mg/kg/d) results in complete tumor growth inhibition in PTEN+/−LKB1+/hypo xenografts without side effects on mice by inhibition of mTORC1 and mTORC2 signaling. [4]

Protocol

Kinase Assay:[1]
+ Expand

Cell-based determinations of mTOR inhibition:

A high-throughput screening cell-based assay is developed using MDA-MB-468 cells to detect mTORC1 and mTORC2 activity. Cells are exposed for 2 hours to increasing concentrations of AZD8055. At the end of the incubation period, cells are fixed, washed, and probed with antibodies against S473 pAkt or against S235/236 phosphorylated S6 (pS6). Levels of phosphorylation are assessed using an Acumen laser scanning cytometer.
Cell Research:[1]
+ Expand
  • Cell lines: U87MG, A549 and H838 cells
  • Concentrations: 1 nM - 1 μM
  • Incubation Time: 72 to 96 hours
  • Method: Cells are exposed to AZD8055 for 72 to 96 hours and stained for cell nuclei (0.03 mg/mL Hoechst 33342) and acidic vesicles (1 μg/mL acridine orange). Images are captured at 450 and 536 nm on an ArrayScan II platform, and the percentage of acidic vesicles and the number of cells are quantified. For LC3 assessment, cells are exposed to e64d/pepstatin (10 μg/mL) for 30 to 90 min before incubation with AZD8055. Cells are lysed on ice and analyzed by immunoblotting.
    (Only for Reference)
Animal Research:[1]
+ Expand
  • Animal Models: U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA U87-MG and A549 are established in pathogen-free, female nude mice (nu/nu:Alpk).
  • Formulation: Dissolved in captisol (CyDex, pH 3.0) and diluted to a final captisol concentration of 30% (w/v)
  • Dosages: 2.5-20 mg/kg
  • Administration: Oral gavage once or twice daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 50 mg/mL warmed (107.4 mM)
Ethanol 3 mg/mL (6.44 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order:
4% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 465.54
Formula

C25H31N5O4

CAS No. 1009298-09-2
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01316809 Completed Glioblastoma Multiforme|Anaplastic Astrocytoma|Anaplastic Oligodendroglioma|Malignant Glioma|Brainstem Glioma National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) March 4, 2011 Phase 1
NCT00999882 Completed Cancer|Advanced Hepatocellular Carcinoma AstraZeneca October 2009 Phase 1
NCT00973076 Completed Cancer|Solid Tumors|Advanced Solid Malignancies AstraZeneca August 2009 Phase 1
NCT00731263 Completed Solid Tumors AstraZeneca July 2008 Phase 1
NCT01194193 Withdrawn Cancer|Advanced Solid Tumours|Lymphomas AstraZeneca null Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID