AZD8055

Catalog No.S1555

AZD8055 Chemical Structure

Molecular Weight(MW): 465.54

AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. Phase 1.

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In DMSO USD 156 In stock
USD 120 In stock
USD 370 In stock
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Cited by 38 Publications

8 Customer Reviews

  • PC3-LN4 cells were treated with GSK690693 (5 uM) alone, or in combination with PP242 (2 uM) or AZD8055 (1 uM) for 24 h, and immunoblotting performed.

    Cancer Res 2013 73(11), 3402-11. AZD8055 purchased from Selleck.

    HUVEC were treated with either RAD001 or AZD8055 before PA treatment. p53, GPX1, p53 acetylation, and subcellular PTEN were analyzed with corresponding antibodies. The blot is a representative of the results of three experiments. Values (mean盨D) are expressed as fold over untreated control. *p<0.05 versus their corresponding control groups in the one-way ANOVA.

    Antioxid Redox Signal 2014 20(9), 1382-95. AZD8055 purchased from Selleck.

  • (A) HCT116 or MDA-MB-468 cells were treated with 500 nM AZD8055 for the indicated times. Cell lysates were immunoblotted with the indicated antibodies. (B) HCT116 or MDA-MB-468 cells were treated with 50 nM rapamycin. 500 nM AZD8055, 1 uM MK2206 or dimethyl sulfoxide (DMSO) as control for 12 h. Cell lysates were immunoblotted with the indicated antibodies.

    Oncotarget 2014 5(15), 6015-27. AZD8055 purchased from Selleck.

    CSCs+24/44/ESA at 5,000 cells per well with stem cell culture media in triplicate in an ultra-low adhesion plate were treated with 5 umol/L BMS-777607, 1 umol/L AZD8055 alone, or in their different combinations. Cells were cultured for 72 hours. Cell viability was determined by the MTS assay. Percentages of polyploid cells were determined by counting 300 cells from two different regions. Results shown here were from one of two experiments with similar results.

    Mol Cancer Ther 2014 13(1), 37-48. AZD8055 purchased from Selleck.

  • mTOR kinase inhibitor AZD8055 activates PI3K accompanied with induction of expression of EGFR, HER2, HER3 and IRS1. Serum-deprived CHO-EGFP-AKT cells were incubated with 50 nM AZD8055 for 24 hr. The EGFP signal was detected using confocal microscopy. The white arrows indicate EGFP-AKT located on cellular membrane.

    Int J Cancer 2014 135(10), 2462-74. AZD8055 purchased from Selleck.

    mTOR, P70S6K1, 4E-BP1 and PCNA activation in the basilar artery of SAH. Top, Western blotting bands of antibodies, bottom, density of the protein band from each group. A, mTOR activation in the basilar artery demonstrated an elevated expression of mTOR on day 7 following SAH in SAH and SAH+DMSO animals. The mTOR inhibitors, Rapamycin and AZD8055 reduced the enhanced expression of mTOR (pb0.05, ANOVA). B. P70S6K1 activation. C. 4E-BP1 activation. D. PCNA activation. Rapamycin and AZD8055 significantly decreased the expression of P70S6K1, 4E-BP1 and PCNA compared with the SAH and SAH+DMSO (pb0.05, respectively; ANOVA). There was no significant difference between the two inhibitors in mTOR, P70S6K1, 4E-BP1 and PCNA expression.

    Exp Neurol 2012 233, 799-806. AZD8055 purchased from Selleck.

  • Induction of TCTP expression through 5-FU or oxaliplatin is inhibited by mTOR kinase inhibitors. HCT116 cells were treated for 48 h with 5-FU or oxaliplatin at the indicated concentrations. Where indicated, AZD 8055 (100 nM) or PP242 (1 μM) were added. TCTP levels were assessed by western blotting; signals were quantified and normalised against α-tubulin as a loading control. As a readout of mTOR activity, phosphorylation of the ribosomal protein S6 at Ser 240/244 was assessed using a phospho-specific antibody. a. Representative western blots demonstrating the alteration of the levels of TCTP protein and of phospho-ribosomal protein S6, respectively, in relation to the drug concentration.

    Cell Commun Signal, 2017, 15(1):9. AZD8055 purchased from Selleck.

    After starved in serum-free medium for 24h , A549 cells incubated with the indicated concentrations of AZD8055 for 3h , followed by 20-minute stimolation of 100ng/ml EGF

     
     

     

    Dr. Zhang of Tianjin Medical University. AZD8055 purchased from Selleck.

Purity & Quality Control

Choose Selective mTOR Inhibitors

Biological Activity

Description AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. Phase 1.
Features First drug to inhibit both types of mTOR protein.
Targets
mTOR (truncated) [1]
(MDA-MB-468 cells)
mTOR (full length) [1]
(MDA-MB-468 cells)
0.13 nM 0.8 nM
In vitro

AZD8055 shows low activity (∼1,000-fold) against all PI3K isoforms (α, β, γ, δ) and other members of the PI3K-like kinase family (ATM and DNA-PK). AZD8055 inhibits the phosphorylation of mTORC1 (p70S6K and 4E-BP1) as well as phosphorylation of the mTORC2 (Akt) and downstream proteins. The rapamycin-resistant T37/46 phosphorylation sites on 4E-BP1 can be fully inhibited by AZD8055, resulting in significant inhibition of cap-dependent translation. AZD8055 potently inhibits proliferation in U87MG, A549 and H838 cells with IC50 of 53, 50 and 20 nM, respectively. AZD8055 also induces autophagy and increased LC3-II levels in H838 and A549 cells. [1] AZD8055 decreases AML blast cell proliferation and cell cycle progression, reduces the clonogenic growth of leukemic progenitors and induces caspase-dependent apoptosis in leukemic cells but not in normal immature CD34+ cells. [2] AZD8055 indicates inhibitory against the pediatric preclinical testing program (PPTP) cell lines with IC50 of 24.7 nM and induces significant differences in EFS distribution. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LU-139 NGDRUJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnzjTWM2OD1yLkCyN|MyKM7:TR?= M3HwSHNCVkeHUh?=
NCI-H720 NGnEN3hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmPJTWM2OD1yLkCyPVM5KM7:TR?= M3HOXnNCVkeHUh?=
KARPAS-45 Mkf0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3zESWlEPTB;MD6wN|M4OSEQvF2= M1rpZXNCVkeHUh?=
EW-3 NHLaUHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2HUSWlEPTB;MD6wOFA2PiEQvF2= NGG0TlJUSU6JRWK=
SBC-1 Mme5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXPT[IdPUUN3ME2wMlA1PThzIN88US=> M2PocXNCVkeHUh?=
NCI-H209 NImyXoJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{fSdWlEPTB;MD6wOVQ2OyEQvF2= NFTQXJBUSU6JRWK=
EoL-1-cell M2DmRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4nHNmlEPTB;MD6wOlU3QSEQvF2= MljUV2FPT0WU
H9 M1fTPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYDiVoxwUUN3ME2wMlA3Pjl{IN88US=> NF3LeGVUSU6JRWK=
AN3-CA MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXzJR|UxRTBwMEe5OVEh|ryP MnfqV2FPT0WU
HH NV;JeItET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH7uTZVKSzVyPUCuNFgxODlizszN MmewV2FPT0WU
KU812 M2rLZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4nYbWlEPTB;MD6wPFA1OyEQvF2= NXXIZ3hFW0GQR1XS
DU-4475 NVjqRlhPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4fF[2lEPTB;MD6wPFA1PyEQvF2= NInWfZZUSU6JRWK=
CTV-1 M2fmdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVfJR|UxRTBwMEi2OFEh|ryP M4\MenNCVkeHUh?=
BV-173 M1:wTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEjiTFhKSzVyPUCuNFkxPjFizszN M1flbXNCVkeHUh?=
ES5 MoHRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEmwdmlKSzVyPUCuNFk{PSEQvF2= MVPTRW5ITVJ?
G-402 NUHwfW9rT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3TjRmlEPTB;MD6wPVk5KM7:TR?= MV3TRW5ITVJ?
MV-4-11 NUm5OGFqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHT0ZYdKSzVyPUCuNVA{PDdizszN MoD4V2FPT0WU
NB69 NVv2PVFWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUjRTVVqUUN3ME2wMlE{PSEQvF2= MW\TRW5ITVJ?
EGI-1 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH;DNlVKSzVyPUCuNVM2OzFizszN M2HJSXNCVkeHUh?=
EW-18 M1O4WGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUTzPHJyUUN3ME2wMlE1ODd|IN88US=> MYjTRW5ITVJ?
ME-180 MnjJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYCyT4pbUUN3ME2wMlE1PjhzIN88US=> M3LS[3NCVkeHUh?=
D-283MED NWTs[ppsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4r2U2lEPTB;MD6xOFczOyEQvF2= NFfvWoxUSU6JRWK=
NB14 NFOxWIdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUPmV5Q3UUN3ME2wMlE3PDR5IN88US=> NUe0NHBrW0GQR1XS
Ramos-2G6-4C10 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlrRTWM2OD1yLkG2PVg{KM7:TR?= NIDseXBUSU6JRWK=
NBsusSR NFn5Oo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWDLZ2kyUUN3ME2wMlE4ODF7IN88US=> MWTTRW5ITVJ?
LAMA-84 NIXZV2RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHXseY1KSzVyPUCuNVgxOzJizszN MY\TRW5ITVJ?
NCI-H1581 M{nNNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWLIZWtXUUN3ME2wMlE5QTZ4IN88US=> M2\Ke3NCVkeHUh?=
A204 NUfYSI5wT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mo\tTWM2OD1yLkG4PVch|ryP NWPxcZRsW0GQR1XS
A549 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUHJR|UxRTBwMUm1OUDPxE1? NVHIUm45W0GQR1XS
CAL-51 MoLLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWD6eHo5UUN3ME2wMlE6PjVzIN88US=> NUjrNWNuW0GQR1XS
NKM-1 MnTrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3nLWGlEPTB;MD6yNFAzQSEQvF2= NVLOR3E5W0GQR1XS
LoVo MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWDJR|UxRTBwMkCyNFIh|ryP MWXTRW5ITVJ?
HAL-01 NVr3NZltT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NW\BSZl{UUN3ME2wMlIxOjl5IN88US=> M4rST3NCVkeHUh?=
HCC2218 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnrWTWM2OD1yLkKwOlg5KM7:TR?= MUDTRW5ITVJ?
BEN MkHQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGPOUXVKSzVyPUCuNlEzQTNizszN NG\jcW1USU6JRWK=
LC-2-ad MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnXoTWM2OD1yLkKyNFg1KM7:TR?= M3jZXnNCVkeHUh?=
LXF-289 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnrMTWM2OD1yLkK1NFQyKM7:TR?= Mn:0V2FPT0WU
HGC-27 NHLpc4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1Tw[WlEPTB;MD6yOlQyPCEQvF2= MWXTRW5ITVJ?
LK-2 M1u1O2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4nqd2lEPTB;MD6yOlgzPCEQvF2= NV3P[lJPW0GQR1XS
CTB-1 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mnv0TWM2OD1yLkK3NlE2KM7:TR?= NXvtT|F3W0GQR1XS
697 MnXhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEPqOnpKSzVyPUCuNlc{PjdizszN NXnObpNWW0GQR1XS
ES7 MkG0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2CzbGlEPTB;MD6yO|M5OyEQvF2= M163T3NCVkeHUh?=
MG-63 M2\PWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGLzbZFKSzVyPUCuNlc1ODdizszN NFfRe5VUSU6JRWK=
T47D MkfsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWnLcFMzUUN3ME2wMlI4PDd3IN88US=> NXeyOo9JW0GQR1XS
RPMI-8226 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYjJR|UxRTBwMki1PFkh|ryP MXLTRW5ITVJ?
NCI-H292 NYLDVIJ[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFLCV4xKSzVyPUCuNlkyPjRizszN NWnFRmtIW0GQR1XS
HT NGH1UGhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWLVU254UUN3ME2wMlI6OzhizszN Ml20V2FPT0WU
A3-KAW M1TqS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVXJR|UxRTBwMkm3OEDPxE1? NVeycZNRW0GQR1XS
LB2241-RCC M2W2V2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3HLb2lEPTB;MD6zNFE6QCEQvF2= NX;5U2ZFW0GQR1XS
BB65-RCC M4\lV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1nGUGlEPTB;MD6zNFgxPSEQvF2= NWDtc3JWW0GQR1XS
G-401 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3X3[2lEPTB;MD6zNFk5PiEQvF2= NHvNTIxUSU6JRWK=
TYK-nu NWTsW|FKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkLQTWM2OD1yLkOxPFY1KM7:TR?= MV7TRW5ITVJ?
NCI-SNU-1 MonTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnjzTWM2OD1yLkOyN|MyKM7:TR?= NGrrbFRUSU6JRWK=
CHL-1 M3njUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYHJR|UxRTBwM{K3NFMh|ryP MnrVV2FPT0WU
P30-OHK MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWLufYk1UUN3ME2wMlMzPzB3IN88US=> MYTTRW5ITVJ?
PA-1 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWnvc|k{UUN3ME2wMlM{PjF7IN88US=> MUjTRW5ITVJ?
LB1047-RCC M3;3[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3LNbmlEPTB;MD6zOFA1PyEQvF2= MUPTRW5ITVJ?
EM-2 NVKxXFlxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYHJR|UxRTBwM{SxPFgh|ryP MUfTRW5ITVJ?
VA-ES-BJ MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlzqTWM2OD1yLkO0NlY5KM7:TR?= MmfZV2FPT0WU
NCI-H520 M4Hoc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHW2UWdKSzVyPUCuN|Q5OzRizszN MoSxV2FPT0WU
ECC10 MmXVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoHZTWM2OD1yLkO1NFM2KM7:TR?= MVrTRW5ITVJ?
LNCaP-Clone-FGC M3fDWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnLGTWM2OD1yLkO1N|E1KM7:TR?= NUO2enNiW0GQR1XS
ES8 NULKUm9ET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NET4[mVKSzVyPUCuN|Y2PTNizszN MUPTRW5ITVJ?
HuO-3N1 Mne2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NELSfGxKSzVyPUCuN|g{PzlizszN Ml\jV2FPT0WU
L-363 NEe1WGxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmTzTWM2OD1yLkO5NFI3KM7:TR?= MU\TRW5ITVJ?
SK-LU-1 MoHpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX3JR|UxRTBwM{myNFgh|ryP NFnGR|hUSU6JRWK=
MCF7 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4HwfGlEPTB;MD60NFU{KM7:TR?= MXzTRW5ITVJ?
HuO9 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXPJR|UxRTBwNEC2Olch|ryP NISyNZRUSU6JRWK=
BHT-101 M4LhOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWjJR|UxRTBwNEC3PFch|ryP M2rBXXNCVkeHUh?=
MZ2-MEL M3LqNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXfJR|UxRTBwNEC4PFIh|ryP NF;0TmpUSU6JRWK=
NOS-1 M3mzWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MW\JR|UxRTBwNEGwO|Qh|ryP MknQV2FPT0WU
NEC8 NGPGNWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mke1TWM2OD1yLkSxOlk2KM7:TR?= MYfTRW5ITVJ?
COR-L88 NX;oSFZmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXjQW|A2UUN3ME2wMlQzOjZ5IN88US=> Mom5V2FPT0WU
MFM-223 NWPMOo1qT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFrSWFlKSzVyPUCuOFI3OTdizszN NGXwZlBUSU6JRWK=
HSC-3 NHzESXNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mke4TWM2OD1yLkSyPVA5KM7:TR?= MlS0V2FPT0WU
OVCAR-5 NVfLO2h{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIK1dYhKSzVyPUCuOFI6OzNizszN M3v2eHNCVkeHUh?=
MFE-280 NYrBXmVjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{nEUWlEPTB;MD60N|A{OiEQvF2= NXT5WpFHW0GQR1XS
KP-N-YS NVzBS|VZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHfne3ZKSzVyPUCuOFMxPyEQvF2= MYnTRW5ITVJ?
EPLC-272H NVvB[Y55T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWXJR|UxRTBwNEOwO|ch|ryP NF:5UoZUSU6JRWK=
KE-37 MlvaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn7zTWM2OD1yLkSzNlQ4KM7:TR?= NX30SZh3W0GQR1XS
HuH-7 NYfOPXM1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4C5NGlEPTB;MD60N|M4OiEQvF2= NYjW[ZIyW0GQR1XS
CHP-212 M3;Wcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1HvT2lEPTB;MD60N|Y6PyEQvF2= NFHMVGlUSU6JRWK=
SK-MES-1 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnGxTWM2OD1yLkSzPFQ5KM7:TR?= MWjTRW5ITVJ?
SJSA-1 Mo\zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYnKNo5vUUN3ME2wMlQ1PTV2IN88US=> MnjyV2FPT0WU
QIMR-WIL NUjyR|VYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHvNUG1KSzVyPUCuOFUyPSEQvF2= M1y3cXNCVkeHUh?=
ES4 NF7wWYZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3vud2lEPTB;MD60OVM{PiEQvF2= MlHhV2FPT0WU
TI-73 M4rJbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUHadotJUUN3ME2wMlQ2Pjh4IN88US=> NUi5S4prW0GQR1XS
COLO-684 NFf1ZYZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2nvfmlEPTB;MD60OVczPiEQvF2= M{e4OXNCVkeHUh?=
GCIY M3q5dmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MULJR|UxRTBwNEW3OVQh|ryP MmqyV2FPT0WU
JVM-3 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoHCTWM2OD1yLkS2NlkzKM7:TR?= M1m1UXNCVkeHUh?=
MPP-89 NGDVRWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWjoZZdtUUN3ME2wMlQ3QTR5IN88US=> M2LFTXNCVkeHUh?=
M14 MlfCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnnRTWM2OD1yLkS3NVch|ryP MUPTRW5ITVJ?
SW962 MnGwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVe1eop3UUN3ME2wMlQ4OTlizszN NVznXmJPW0GQR1XS
VM-CUB-1 NHHzRm1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXvJR|UxRTBwNEeyPFYh|ryP MWPTRW5ITVJ?
A101D MmTLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXzJR|UxRTBwNEi5Olgh|ryP MmPYV2FPT0WU
DB NUXuRWJqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWW0OXVnUUN3ME2wMlUxODJzIN88US=> MkfnV2FPT0WU
KNS-62 NUW3[XJuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIDUN4ZKSzVyPUCuOVA2PjhizszN NV\PNpY5W0GQR1XS
NCI-H460 NVfoNm1QT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHnrXmpKSzVyPUCuOVA2QTdizszN NXXTSYNxW0GQR1XS
MDA-MB-361 NF30VFdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUHJR|UxRTBwNUC4O{DPxE1? MUPTRW5ITVJ?
DOK NXvSS3dxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHXCR2dKSzVyPUCuOVE{OzNizszN M1v0bXNCVkeHUh?=
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Ca-Ski M2XMOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmrsTWM2OD13Lk[yOVY3KM7:TR?= M3jG[XNCVkeHUh?=
COLO-741 NVvOWFd3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnzLTWM2OD13LkexPVk2KM7:TR?= NYrGNlBsW0GQR1XS
HT-29 M4jTW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3z6cGlEPTB;NT64NFQ4PSEQvF2= NYXSSYppW0GQR1XS
HCC1187 NYXTRYxlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3OyWmlEPTB;NT64NVAxQCEQvF2= NXzQcJpXW0GQR1XS
Capan-2 NHTYcGtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVrIVXZRUUN3ME21Mlk2QTl2IN88US=> M4jnRXNCVkeHUh?=
NCI-H630 MkSzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYXQUnZkUUN3ME22MlEyPDRzIN88US=> M1voRXNCVkeHUh?=
COLO-678 MmLUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYDJR|UxRTZwMUWwPVIh|ryP MYnTRW5ITVJ?
D-502MG Mo[xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MULJR|UxRTZwMUezN|Yh|ryP NWC3S2dVW0GQR1XS
NB17 M4DXT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MonZTWM2OD14LkK0PVQ{KM7:TR?= NWDMfnJ6W0GQR1XS
SK-MEL-24 M37meWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M17iU2lEPTB;Nj6yOlQ6KM7:TR?= NEG2eZlUSU6JRWK=
NCI-SNU-5 MoK0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NELYZ4dKSzVyPU[uNlcyOThizszN M2O0cXNCVkeHUh?=
D-392MG MonDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXLJR|UxRTZwM{OzPVkh|ryP Ml:1V2FPT0WU
A498 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3\0ZWlEPTB;Nj60NFMxOiEQvF2= NX74Ro9PW0GQR1XS
OCI-AML2 NWTMeHc3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2XRW2lEPTB;Nj60OVQ1PSEQvF2= NWrrVnZrW0GQR1XS
HCC2157 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmnsTWM2OD14LkS1OlE3KM7:TR?= MXnTRW5ITVJ?
C3A MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYSyTJcxUUN3ME22MlUzODB2IN88US=> M1nKNXNCVkeHUh?=
NCI-H1650 M37BN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NI\DSVBKSzVyPU[uO|A2OjJizszN MXLTRW5ITVJ?
DU-145 M2jDOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYHJR|UxRTZwN{ewN{DPxE1? NXz4TJRbW0GQR1XS
COLO-829 NXziblRwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVruR4JVUUN3ME22Mlc4QDd|IN88US=> M1\oN3NCVkeHUh?=
LS-1034 MkLCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3j4[2lEPTB;Nz6wOFQyOiEQvF2= NVLDe3dsW0GQR1XS
HCC1954 M3O4Nmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1\zN2lEPTB;Nz60PFE{PCEQvF2= M2PLVXNCVkeHUh?=
KURAMOCHI NVrFV4RqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{\3PGlEPTB;Nz60PFU{OyEQvF2= NGfEb4pUSU6JRWK=
Mo-T M3Tib2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2DWNWlEPTB;Nz61NVQ6QCEQvF2= M3HRUXNCVkeHUh?=
OE19 MnnCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWTQdZRtUUN3ME23MlY2PTB|IN88US=> NVn4d4hwW0GQR1XS
COR-L105 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXfJR|UxRTdwN{G0NlEh|ryP NFyybWRUSU6JRWK=
NCI-H2342 M3\tcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWHLVlN4UUN3ME23MlcyPTJzIN88US=> NFTseIlUSU6JRWK=
U-87-MG M{fpbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH\xfWJKSzVyPUiuNFM{OzRizszN MnLOV2FPT0WU
MKN28 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVvYNFlHUUN3ME24MlEyPTF3IN88US=> NFuzZY1USU6JRWK=
HT-1197 MkPPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYPHXoZXUUN3ME24MlI2QTB5IN88US=> NHPqZpZUSU6JRWK=
ChaGo-K-1 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFLUeXlKSzVyPUiuN|QzPDVizszN M1nDdnNCVkeHUh?=
NCI-H226 NHvuO3NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXG4eHN2UUN3ME24MlQ1OzNzIN88US=> NET2bodUSU6JRWK=
NCI-H2029 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1nZTWlEPTB;OT6wOlc2QSEQvF2= M1O4WXNCVkeHUh?=
TE-11 NFfDOJRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{jOcGlEPTB;OT60OVU5QCEQvF2= NUfu[VNOW0GQR1XS
ZR-75-30 NVjqUYpjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF\zSFZKSzVyPUmuPVI3OzRizszN MkfWV2FPT0WU
SW837 MmC5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3H5c2lEPTB;MUCuNlI6OyEQvF2= MlSyV2FPT0WU
NCI-H1651 MoPHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4HhOmlEPTB;MUCuNlMzPiEQvF2= MlvPV2FPT0WU
NCI-H727 MlfrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnHsTWM2OD1zMD60OFk2KM7:TR?= NUG0PGx4W0GQR1XS
NCI-H358 NYPURlhRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{DBeWlEPTB;MUCuPFM3PSEQvF2= MVrTRW5ITVJ?
NCI-H2087 NELWe|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmTUTWM2OD1zMT6wOFM1KM7:TR?= MlXWV2FPT0WU
FTC-133 Ml3BS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYDJR|UxRTFzLkSwOVEh|ryP NHm3PXdUSU6JRWK=
LAN-6 M{TqRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1nuW2lEPTB;MUKuNVI1KM7:TR?= NYPtfZY5W0GQR1XS
NCI-H1838 Mkf1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1fRU2lEPTB;MUKuOVE2OSEQvF2= M1roNnNCVkeHUh?=
Calu-6 NIDFeGhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWjVeW1QUUN3ME2xOE4yODJzIN88US=> NUfhZ|NvW0GQR1XS
HCC70 NFHXfFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUnQ[mM3UUN3ME2xOE41PTFizszN NX;MO|VOW0GQR1XS
KARPAS-299 M3PuOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVmwZnJ2UUN3ME2xOU4zOTV7IN88US=> NFfx[XFUSU6JRWK=
CAL-27 M3jucmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3nY[WlEPTB;MUWuN|g1OyEQvF2= MkDXV2FPT0WU
NCI-H82 NGrlW|lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MULJR|UxRTF3LkWzNkDPxE1? MYLTRW5ITVJ?
no-10 M1H2cGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFnWPYNKSzVyPUG1Mlk{QCEQvF2= Mnj4V2FPT0WU
U251 M4Xqcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NInOeFJKSzVyPUG2MlM3PzVizszN NGrGT4dUSU6JRWK=
TE-9 M160PGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYnIV21uUUN3ME2xO{42OTZ4IN88US=> NYGyTJBZW0GQR1XS
TE-10 NYS0PId1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmHwTWM2OD1zOT6wOlA4KM7:TR?= NGnNZVZUSU6JRWK=
COLO-824 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3zLUGlEPTB;MkCuPVI4PiEQvF2= NIPjOYpUSU6JRWK=
HuP-T3 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFPCVGhKSzVyPUKxMlA4PDhizszN NU\hdGh4W0GQR1XS
KALS-1 NH\DdZlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYHVTY9vUUN3ME2yNU4yPzVizszN MoLQV2FPT0WU
L-428 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn:yTWM2OD1{MT60NFE3KM7:TR?= NYSxT|VLW0GQR1XS
JAR MlXOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmrOTWM2OD1{MT61NFIh|ryP NVy1RpE5W0GQR1XS
DoTc2-4510 M{HLdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoPITWM2OD1{Mz64OFc2KM7:TR?= MXnTRW5ITVJ?
ABC-1 NVjYdIJVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NI[ySGlKSzVyPUK2Mlc6PTdizszN NY\kRXFQW0GQR1XS
MHH-NB-11 NXPWRWJGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFvoVYJKSzVyPUOzMlM1PjRizszN MYLTRW5ITVJ?
SW1463 M2K4OGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV7SZm01UUN3ME2zN{46QDNizszN MXXTRW5ITVJ?
SW1417 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYXJXHpFUUN3ME2zO{4xQTV{IN88US=> NULBdnJOW0GQR1XS
NB12 NFy5c3VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEC4OZdKSzVyPUO5MlQ3PTVizszN Ml7zV2FPT0WU
NY Ml:0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4ryPGlEPTB;NEGuN|IxOSEQvF2= NX3hNYtZW0GQR1XS
H1048 MWTBdI9xfG:|aYOgRZN{[Xl? MXe1NFAhdk1? Mki1O|IhcA>? Ml\3cohidmOnczDBRnQuOjZ|4pETbY5lfWOnZDDhdI9xfG:|aYO= NGW5bIIzPTd|N{W0Ni=>
H86 MkX6RZBweHSxc3nzJGF{e2G7 NVvue3h[PTByIH7N NXzBXHdWPzJiaB?= M2XpZY5p[W6lZYOgRWJVNTJ4M,MAl4lv\HWlZXSgZZBweHSxc3nz NH7I[5MzPTd|N{W0Ni=>
H196 MlLsRZBweHSxc3nzJGF{e2G7 MV[1NFAhdk1? MmjNO|IhcA>? NF\Oe2VvcGGwY3XzJGFDXC1{NkRihLNqdmS3Y3XkJIFxd3C2b4Ppdy=> M3HEcVI2PzN5NUSy
H446 MkXTRZBweHSxc3nzJGF{e2G7 MWG1NFAhdk1? NV\0[GtMPzJiaB?= NHvkRWhvcGGwY3XzJGFDXC1{NkRihLNqdmS3Y3XkJIFxd3C2b4Ppdy=> M2LU[FI2PzN5NUSy
TFK-1 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmfUO|IhcA>? NVjXR5U2TE2VTx?= MkfxTWM2OD12NzDuUS=> NYPtNlY2OjVzNUKyOFQ>
EGI-1 NFHYVlNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmHxO|IhcA>? M4LzUGROW09? NGDLWW1KSzVyPUWzJI5O MoWzNlUyPTJ{NES=
OCI/AML3 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV7tPXhDOC5{IN88US=> MnX4OFghcA>? NX7CTnA4\W6qYX7j[ZMh[2WubDDndo94fGhiaX7obYJqfGmxbjDpcoR2[2WmIHL5JJNmdHWvZYTpcolj MVyyOFc{QTN7Mx?=
U937 MnXyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3\n[VAvOiEQvF2= MnLJOFghcA>? MVzlcohidmOnczDj[YxtKGe{b4f0bEBqdmirYnn0bY9vKGmwZIXj[YQh[nlic3XseY1mfGmwaXK= MXSyOFc{QTN7Mx?=
MOLM13 MoS0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmfHNE4zKM7:TR?= MkHtOFghcA>? MnTK[Y5p[W6lZYOgZ4VtdCCpcn;3eIghcW6qaXLpeIlwdiCrbnT1Z4VlKGK7IIPlcJVu\XSrbnni MUSyOFc{QTN7Mx?=
MCF7 NFO4S|lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1OzeFIxKG6P NXnJPIhNPzJiaB?= NVjuTGs5TkKV MXTy[YR2[2W|IITo[UBRTUySMT3t[YRq[XSnZDDpcoNz\WG|ZTDpckBk\WyuII\pZYJqdGm2eR?= NUTsbGM2OjR4OEiwOFY>
TamR NV\6RpV4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1:1U2lEPTB;MUlihKlvVQ>? MlnnNlQ1PTdyNkm=
MCF7-X  M1fucWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIW1NWlKSzVyPUK0JI5O MkntNlQ1PTdyNkm=
T47D-tamR  MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUjJR|UxRTF7IH7N M1PRTVI1PDV5ME[5
293T  Mny0SpVv[3Srb36gRZN{[Xl? NVX0[VBFOjBibl2= M1ywRlMhcA>? Mn7S[Il{enWydIOgRYt1NW2nZHnheIVlKFSLRj3JRUB{fGGkaXzpeJktKHS{YX7zcI9k[XSrb36sJIFv\CCjY4Tpeol1gQ>? NYfuSVd4OjR|NkO0OFk>
SW620 M2ntRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2PyT|AvODBzLUGw{txO M1nUcFI1KGh? M2XrTIlvcGmkaYTzJJRp\SClZXzsJJBzd2yrZnXyZZRqd25iYYOgeIhmKGOxbnPlcpRz[XSrb36gdol{\XN? NF21UHQzPDN4M{S0PS=>
Huh7  NGrOfG9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NI\sUXg2NTFyMDDuUS=> NVm4PJRbOC1{NDDo NFLlfHhFVVOR NFS2NmJqdmO{ZXHz[ZMh[2WubDDk[YF1cCCrbjDhJINwdmOnboTyZZRqd25vIHHu[EB1cW2nLXTldIVv\GWwdDDtZY5v\XJ? NHzXXXgzPDJ7N{OwNC=>
Hep3B  NIrpR2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mom4OU0yODBibl2= M2TkOFAuOjRiaB?= NFyweItFVVOR NYfwflVicW6lcnXhd4V{KGOnbHyg[IVifGhiaX6gZUBkd26lZX70doF1cW:wLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{ Ml;3NlQzQTd|MEC=
Huh7  NHqzUYNHfW6ldHnvckBCe3OjeR?= Mo\GNVAxKG6P MWixNkBp NW[2T5RJTE2VTx?= NV3udmh2cW6mdXPld{BifXSxcHjh[5nDqA>? NHXVU4wzPDJ7N{OwNC=>
U87-MG  NHHWZWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlzhO|IhcA>? MnjQS2k2OOLCiU5ihKk4QSCwTdMg M1XsRVI1OjR7NkSw
 L3.6pl NVzGdpBwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVLvTmR4OSEQvF2= MXm3NkBp MoDrbY5pcWKrdIOgeIhmKGOnbHyg[5Jwf3SqIHPvcYJqdmWmIIfpeIghSk2VLUe3O|YxPw>? NX6wR3NTOjR{M{OzPVk>
TE671 MV7BdI9xfG:|aYOgRZN{[Xl? M1n0SVEh|ryP M1HlW|Q5KGh? MY\pcoNz\WG|ZYOgZ4VtdCCjcH;weI9{cXNiaX7keYNm\CCkeTDBRnQuPzN5 M3m2[VI1OTN|MkG4
RD MnL1RZBweHSxc3nzJGF{e2G7 NGL4b5QyKM7:TR?= MWS0PEBp NVnhPZBbcW6lcnXhd4V{KGOnbHygZZBweHSxc3nzJIlv\HWlZXSgZpkhSUKWLUezOy=> MmH4NlQyOzN{MUi=
RMS13 NH7LPZBCeG:ydH;zbZMhSXO|YYm= Moj2NUDPxE1? NWP1bo1CPDhiaB?= MmH2bY5kemWjc3XzJINmdGxiYYDvdJRwe2m|IHnu[JVk\WRiYomgRWJVNTd|Nx?= NILHUlEzPDF|M{KxPC=>
RH30 NVvTUINQSXCxcITvd4l{KEG|c3H5 MUSxJO69VQ>? NVS4fIRLPDhiaB?= NGe3bGtqdmO{ZXHz[ZMh[2WubDDhdI9xfG:|aYOgbY5lfWOnZDDifUBCSlRvN{O3 NU\pOWhZOjRzM{OyNVg>
Hep-2 Ml\mS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGnDZWwxNjBzLUGg{txO NYH5emNQOTJvOU[gbC=> NVHUO3BOcW6lcnXhd4V{KGOnbHyg[IVifGhiaX6gZUBkd26lZX70doF1cW:wLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{ NFKwNHgzPDFyM{e0PS=>
SCC-9 NVHSbItoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWiwcoJMOC5yMT2xJO69VQ>? NXzB[GNSOTJvOU[gbC=> NHza[2tqdmO{ZXHz[ZMh[2WubDDk[YF1cCCrbjDhJINwdmOnboTyZZRqd25vIHHu[EB1cW2nLXTldIVv\GWwdDDtZY5v\XJ? NVjDNJhJOjRzMEO3OFk>
Hep-2 MkTMSpVv[3Srb36gRZN{[Xl? MVOwMlEwOC53IN88US=> NHToN3czPCCq M2X5foJtd2OtczDtWG9TSzFiYX7kJI1VV1KFMjDhZ5RqfmG2aX;u NV3ReWpmOjRzMEO3OFk>
SCC-9 Mn\pSpVv[3Srb36gRZN{[Xl? NIDqcWQxNjFxMD61JO69VQ>? MV:yOEBp Ml;LZoxw[2u|IH3UU3JEOSCjbnSgcXRQWkN{IHHjeIl3[XSrb36= NHLs[lIzPDFyM{e0PS=>
PANC-1 M1zxUmFxd3C2b4Ppd{BCe3OjeR?= MX[1NFAhdk1? NH[4[|U1QCCq M4rt[ZNmdnOrdHn6[ZMheGGwY4LlZZRq[yClYX7j[ZIh[2WubIOgeI8hemGmaX;0bIVz[XC7 MYKyN|g5PjJ7NB?=
RMG2 NGKzOHlCeG:ydH;zbZMhSXO|YYm= NV\E[4pnOS1zMECwJI5O MXG3NkBp MmfCSmJU MYTpcohq[mm2czD0bIUh[2WubDD2bYFjcWyrdImgZ49v[2WwdILheIlwdiCmZYDlcoRmdnSueR?= MWKyN|YyPTZ|MR?=
HAC2 MmLmRZBweHSxc3nzJGF{e2G7 M3XyN|EuOTByMDDuUS=> M1:2TlczKGh? NYX4O21sTkKV NXHCO5N7cW6qaXLpeJMhfGinIHPlcIwhfmmjYnnsbZR6KGOxbnPlcpRz[XSrb36g[IVx\W6mZX70cJk> NF\xVFczOzZzNU[zNS=>
HT-29 MV\BdI9xfG:|aYOgRZN{[Xl? MlW1NUDPxE1? NXjpW|JwPzJiaB?= MlLFZZR1\W63YYTld{A2NU[XLXnu[JVk\WRiY3XscEBl\WG2aB?= MWGyNVk1QTF{MR?=
DLD1 M2fCWWFxd3C2b4Ppd{BCe3OjeR?= NWi3dINrOSEQvF2= MoDCO|IhcA>? NXrjPHA3[XS2ZX71ZZRmeyB3LV\VMYlv\HWlZXSgZ4VtdCCmZXH0bC=> NYDoW3l1OjF7NEmxNlE>
HT-29 NFK0OZJHfW6ldHnvckBCe3OjeR?= NYCyWFE{OSEQvF2= M2PTflczKGh? M2LFZ4lv\HWlZYOgZZV1d3CqYXf5JJRwKGOxbn\ldkBkgXSxcILveIVkfGmxbh?= NIjkZnMzOTl2OUGyNS=>
DLD1 M3Lve2Z2dmO2aX;uJGF{e2G7 NFXFOmQyKM7:TR?= MnnvO|IhcA>? NEDrR2lqdmS3Y3XzJIF2fG:yaHHnfUB1dyClb37m[ZIh[3m2b4Dyc5Rm[3Srb36= MnPSNlE6PDlzMkG=
Rh41 NYXhNolOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVK0[5k5UUN3ME2zNE44KG6P NIflS28zOTN|N{[3PS=>
Rh18 M2rMfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXrYcWFmUUN3ME20NU41KG6P MmLmNlE{Ozd4N{m=
Rh30 MoTWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1K0dWlEPTB;MUSgcm0> NG\aRlQzOTN|N{[3PS=>
BT-12 Mo\ZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NI\MWFBKSzVyPUK0Mlchdk1? NHTPPY8zOTN|N{[3PS=>
CHLA-266 MoLRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVj0ZmpMUUN3ME2xMlEhdk1? Mn;DNlE{Ozd4N{m=
TC-71 NWHIdoFpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml\YTWM2OD12Nj61JI5O NWfwWnpKOjF|M{e2O|k>
CHLA-9 Ml7lS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUfJR|UxRTF3LkOgcm0> NU\V[JNDOjF|M{e2O|k>
CHLA-10 M1TMV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml3pTWM2OD1|Lkmgcm0> NVTudJpZOjF|M{e2O|k>
CHLA-258 MoLJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{PJeGlEPTB;MUCuOEBvVQ>? M2PIdVIyOzN5Nke5
GBM2 NF3hS|RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmPTTWM2OD12MD63JI5O NGDmZnkzOTN|N{[3PS=>
NB-1643 Ml;xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUjh[IplUUN3ME2zN{45KG6P NY\l[WJ2OjF|M{e2O|k>
NB-EBc1 NXnqeVhCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXfMVINsUUN3ME2yO{4yKG6P NGC5N3kzOTN|N{[3PS=>
CHLA-90 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWHWTGpsUUN3ME2zOE42KG6P MoG1NlE{Ozd4N{m=
CHLA-136 NFv6PHRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYXJR|UxRTF4Mj62JI5O M2fSZlIyOzN5Nke5
NALM-6 MkOyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF:0fpBKSzVyPUOyMlkhdk1? NIHPSYIzOTN|N{[3PS=>
COG-LL-317 MlfhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXrJR|UxRTFwMzDuUS=> NVfJXFVLOjF|M{e2O|k>
RS4;11 Mnm0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn\mTWM2OD1|NTDuUS=> MUWyNVM{PzZ5OR?=
MOLT-4 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3\IcmlEPTB;OD62JI5O NFPwRZUzOTN|N{[3PS=>
CCRF-CEM M4GydGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MULJR|UxRTRwMTDuUS=> MYKyNVM{PzZ5OR?=
Kasumi-1 Mo\PS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NECwcmZKSzVyPUGuOEBvVQ>? MUeyNVM{PzZ5OR?=
Karpas-299 MoDjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M33kR2lEPTB;MkGuO{BvVQ>? NIDUR3gzOTN|N{[3PS=>
Ramos-RA1 Mk\DS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkHqTWM2OD16LkKgcm0> MkHSNlE{Ozd4N{m=
H838 M{LwfGZ2dmO2aX;uJGF{e2G7 NFntW5MyKM7:TR?= NVu2cmZjPDhiaB?= MXHpcoR2[2W|IHPlcIwh[XW2b4DoZYd6 NH7YRlIzODN4NEGxNy=>
H838 M4fBVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWCwMVMh|ryP NWeyOHROPzJiaB?= NWPNNXA6cW6mdXPld{Boem:5dHigbY5pcWKrdHnvckBidmRiYYX0c5Bp[We7 NGHpXJMzODB{OEi1OC=>
A549  MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4P5bFAuOyEQvF2= NXTteFRMPzJiaB?= NX3ZPFhKcW6mdXPld{Boem:5dHigbY5pcWKrdHnvckBidmRiYYX0c5Bp[We7 NYrXSnNVOjByMki4OVQ>

... Click to View More Cell Line Experimental Data

In vivo AZD8055 inhibits the pS6 and pAkt in U87MG and A549 xenografts at 2.5/10 mg/kg, which leads to tumor growth inhibition. AZD8055 shows significant antitumor activity in many xenografts, including U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA at a dose of 10/20 mg/kg. [1] AZD8055 induces ~40% reduction in tumour volume, accompanied by ablation of phosphorylation of Akt, S6K and SGK protein kinases. Administration of AZD8055 (5mg/kg, Bid) and SAHA (100 mg/kg/d) results in complete tumor growth inhibition in PTEN+/−LKB1+/hypo xenografts without side effects on mice by inhibition of mTORC1 and mTORC2 signaling. [4]

Protocol

Kinase Assay:[1]
+ Expand

Cell-based determinations of mTOR inhibition:

A high-throughput screening cell-based assay is developed using MDA-MB-468 cells to detect mTORC1 and mTORC2 activity. Cells are exposed for 2 hours to increasing concentrations of AZD8055. At the end of the incubation period, cells are fixed, washed, and probed with antibodies against S473 pAkt or against S235/236 phosphorylated S6 (pS6). Levels of phosphorylation are assessed using an Acumen laser scanning cytometer.
Cell Research:[1]
+ Expand
  • Cell lines: U87MG, A549 and H838 cells
  • Concentrations: 1 nM - 1 μM
  • Incubation Time: 72 to 96 hours
  • Method: Cells are exposed to AZD8055 for 72 to 96 hours and stained for cell nuclei (0.03 mg/mL Hoechst 33342) and acidic vesicles (1 μg/mL acridine orange). Images are captured at 450 and 536 nm on an ArrayScan II platform, and the percentage of acidic vesicles and the number of cells are quantified. For LC3 assessment, cells are exposed to e64d/pepstatin (10 μg/mL) for 30 to 90 min before incubation with AZD8055. Cells are lysed on ice and analyzed by immunoblotting.
    (Only for Reference)
Animal Research:[1]
+ Expand
  • Animal Models: U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA U87-MG and A549 are established in pathogen-free, female nude mice (nu/nu:Alpk).
  • Formulation: Dissolved in captisol (CyDex, pH 3.0) and diluted to a final captisol concentration of 30% (w/v)
  • Dosages: 2.5-20 mg/kg
  • Administration: Oral gavage once or twice daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 50 mg/mL warmed (107.4 mM)
Ethanol 3 mg/mL (6.44 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
4% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 465.54
Formula

C25H31N5O4

CAS No. 1009298-09-2
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
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    Volume
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01316809 Completed Glioblastoma Multiforme|Anaplastic Astrocytoma|Anaplastic Oligodendroglioma|Malignant Glioma|Brainstem Glioma National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) March 4, 2011 Phase 1
NCT00999882 Completed Cancer|Advanced Hepatocellular Carcinoma AstraZeneca October 2009 Phase 1
NCT00973076 Completed Cancer|Solid Tumors|Advanced Solid Malignancies AstraZeneca August 2009 Phase 1
NCT00731263 Completed Solid Tumors AstraZeneca July 2008 Phase 1
NCT01194193 Withdrawn Cancer|Advanced Solid Tumours|Lymphomas AstraZeneca null Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID