AZD8055

Catalog No.S1555

AZD8055 Chemical Structure

Molecular Weight(MW): 465.54

AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. Phase 1.

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In DMSO USD 156 In stock
USD 120 In stock
USD 370 In stock
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Cited by 38 Publications

8 Customer Reviews

  • PC3-LN4 cells were treated with GSK690693 (5 uM) alone, or in combination with PP242 (2 uM) or AZD8055 (1 uM) for 24 h, and immunoblotting performed.

    Cancer Res 2013 73(11), 3402-11. AZD8055 purchased from Selleck.

    HUVEC were treated with either RAD001 or AZD8055 before PA treatment. p53, GPX1, p53 acetylation, and subcellular PTEN were analyzed with corresponding antibodies. The blot is a representative of the results of three experiments. Values (mean盨D) are expressed as fold over untreated control. *p<0.05 versus their corresponding control groups in the one-way ANOVA.

    Antioxid Redox Signal 2014 20(9), 1382-95. AZD8055 purchased from Selleck.

  • (A) HCT116 or MDA-MB-468 cells were treated with 500 nM AZD8055 for the indicated times. Cell lysates were immunoblotted with the indicated antibodies. (B) HCT116 or MDA-MB-468 cells were treated with 50 nM rapamycin. 500 nM AZD8055, 1 uM MK2206 or dimethyl sulfoxide (DMSO) as control for 12 h. Cell lysates were immunoblotted with the indicated antibodies.

    Oncotarget 2014 5(15), 6015-27. AZD8055 purchased from Selleck.

    CSCs+24/44/ESA at 5,000 cells per well with stem cell culture media in triplicate in an ultra-low adhesion plate were treated with 5 umol/L BMS-777607, 1 umol/L AZD8055 alone, or in their different combinations. Cells were cultured for 72 hours. Cell viability was determined by the MTS assay. Percentages of polyploid cells were determined by counting 300 cells from two different regions. Results shown here were from one of two experiments with similar results.

    Mol Cancer Ther 2014 13(1), 37-48. AZD8055 purchased from Selleck.

  • mTOR kinase inhibitor AZD8055 activates PI3K accompanied with induction of expression of EGFR, HER2, HER3 and IRS1. Serum-deprived CHO-EGFP-AKT cells were incubated with 50 nM AZD8055 for 24 hr. The EGFP signal was detected using confocal microscopy. The white arrows indicate EGFP-AKT located on cellular membrane.

    Int J Cancer 2014 135(10), 2462-74. AZD8055 purchased from Selleck.

    mTOR, P70S6K1, 4E-BP1 and PCNA activation in the basilar artery of SAH. Top, Western blotting bands of antibodies, bottom, density of the protein band from each group. A, mTOR activation in the basilar artery demonstrated an elevated expression of mTOR on day 7 following SAH in SAH and SAH+DMSO animals. The mTOR inhibitors, Rapamycin and AZD8055 reduced the enhanced expression of mTOR (pb0.05, ANOVA). B. P70S6K1 activation. C. 4E-BP1 activation. D. PCNA activation. Rapamycin and AZD8055 significantly decreased the expression of P70S6K1, 4E-BP1 and PCNA compared with the SAH and SAH+DMSO (pb0.05, respectively; ANOVA). There was no significant difference between the two inhibitors in mTOR, P70S6K1, 4E-BP1 and PCNA expression.

    Exp Neurol 2012 233, 799-806. AZD8055 purchased from Selleck.

  • Induction of TCTP expression through 5-FU or oxaliplatin is inhibited by mTOR kinase inhibitors. HCT116 cells were treated for 48 h with 5-FU or oxaliplatin at the indicated concentrations. Where indicated, AZD 8055 (100 nM) or PP242 (1 μM) were added. TCTP levels were assessed by western blotting; signals were quantified and normalised against α-tubulin as a loading control. As a readout of mTOR activity, phosphorylation of the ribosomal protein S6 at Ser 240/244 was assessed using a phospho-specific antibody. a. Representative western blots demonstrating the alteration of the levels of TCTP protein and of phospho-ribosomal protein S6, respectively, in relation to the drug concentration.

    Cell Commun Signal, 2017, 15(1):9. AZD8055 purchased from Selleck.

    After starved in serum-free medium for 24h , A549 cells incubated with the indicated concentrations of AZD8055 for 3h , followed by 20-minute stimolation of 100ng/ml EGF

     
     

     

    Dr. Zhang of Tianjin Medical University. AZD8055 purchased from Selleck.

Purity & Quality Control

Choose Selective mTOR Inhibitors

Biological Activity

Description AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. Phase 1.
Features First drug to inhibit both types of mTOR protein.
Targets
mTOR (truncated) [1]
(MDA-MB-468 cells)
mTOR (full length) [1]
(MDA-MB-468 cells)
0.13 nM 0.8 nM
In vitro

AZD8055 shows low activity (∼1,000-fold) against all PI3K isoforms (α, β, γ, δ) and other members of the PI3K-like kinase family (ATM and DNA-PK). AZD8055 inhibits the phosphorylation of mTORC1 (p70S6K and 4E-BP1) as well as phosphorylation of the mTORC2 (Akt) and downstream proteins. The rapamycin-resistant T37/46 phosphorylation sites on 4E-BP1 can be fully inhibited by AZD8055, resulting in significant inhibition of cap-dependent translation. AZD8055 potently inhibits proliferation in U87MG, A549 and H838 cells with IC50 of 53, 50 and 20 nM, respectively. AZD8055 also induces autophagy and increased LC3-II levels in H838 and A549 cells. [1] AZD8055 decreases AML blast cell proliferation and cell cycle progression, reduces the clonogenic growth of leukemic progenitors and induces caspase-dependent apoptosis in leukemic cells but not in normal immature CD34+ cells. [2] AZD8055 indicates inhibitory against the pediatric preclinical testing program (PPTP) cell lines with IC50 of 24.7 nM and induces significant differences in EFS distribution. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LU-139 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWPJR|UxRTBwMEKzN|Eh|ryP MYPTRW5ITVJ?
NCI-H720 MnHMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1f6ZWlEPTB;MD6wNlk{QCEQvF2= NV7BZWZnW0GQR1XS
KARPAS-45 NHThZoFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M37k[mlEPTB;MD6wN|M4OSEQvF2= NGHrWZJUSU6JRWK=
EW-3 NV:3TYpnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{fjbmlEPTB;MD6wOFA2PiEQvF2= M3jSSHNCVkeHUh?=
SBC-1 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mk[zTWM2OD1yLkC0OVgyKM7:TR?= NEP3ZXhUSU6JRWK=
NCI-H209 NV3vRlhST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoDETWM2OD1yLkC1OFU{KM7:TR?= MojMV2FPT0WU
EoL-1-cell MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYnROFBvUUN3ME2wMlA3PTZ7IN88US=> NEL5OVlUSU6JRWK=
H9 M4X1dGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4HEVGlEPTB;MD6wOlY6OiEQvF2= NHKwPWRUSU6JRWK=
AN3-CA NHTIcXlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUnJR|UxRTBwMEe5OVEh|ryP NFXHSHFUSU6JRWK=
HH NIm4UmZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MULJR|UxRTBwMEiwNFkh|ryP MnzhV2FPT0WU
KU812 NVntWmxXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoPUTWM2OD1yLkC4NFQ{KM7:TR?= NFy0bXBUSU6JRWK=
DU-4475 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnPDTWM2OD1yLkC4NFQ4KM7:TR?= Mkj1V2FPT0WU
CTV-1 NFfDOYdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NU[3S2hEUUN3ME2wMlA5PjRzIN88US=> NUjLe4RlW0GQR1XS
BV-173 M{HOVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{W0cmlEPTB;MD6wPVA3OSEQvF2= NEO1UIRUSU6JRWK=
ES5 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX;JR|UxRTBwMEmzOUDPxE1? NYrqRYpPW0GQR1XS
G-402 M4HKWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3LPOWlEPTB;MD6wPVk5KM7:TR?= MkDPV2FPT0WU
MV-4-11 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV7JR|UxRTBwMUCzOFch|ryP NGTSSoZUSU6JRWK=
NB69 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEDrXohKSzVyPUCuNVM2KM7:TR?= MWfTRW5ITVJ?
EGI-1 MmfIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWH5flZiUUN3ME2wMlE{PTNzIN88US=> MWXTRW5ITVJ?
EW-18 NVn3e2dnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIfUXmhKSzVyPUCuNVQxPzNizszN NFO4ZY5USU6JRWK=
ME-180 M3HmR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoH3TWM2OD1yLkG0OlgyKM7:TR?= MkfxV2FPT0WU
D-283MED NE\Xe5FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVHTb5NJUUN3ME2wMlE1PzJ|IN88US=> NXfo[2hkW0GQR1XS
NB14 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX\JR|UxRTBwMU[0OFch|ryP MULTRW5ITVJ?
Ramos-2G6-4C10 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGH5NG9KSzVyPUCuNVY6QDNizszN NILERZdUSU6JRWK=
NBsusSR NXTGW3FiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGC4WYlKSzVyPUCuNVcxOTlizszN MYXTRW5ITVJ?
LAMA-84 NHPKWFRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYXPZow2UUN3ME2wMlE5ODN{IN88US=> M{nFeXNCVkeHUh?=
NCI-H1581 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV;JR|UxRTBwMUi5OlYh|ryP NXPiUZlQW0GQR1XS
A204 NUPSTFdvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn;1TWM2OD1yLkG4PVch|ryP MXnTRW5ITVJ?
A549 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHy2UpVKSzVyPUCuNVk2PSEQvF2= NWnhbZh7W0GQR1XS
CAL-51 M3HxRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUDsbYlZUUN3ME2wMlE6PjVzIN88US=> M37jWXNCVkeHUh?=
NKM-1 MnfqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mkm4TWM2OD1yLkKwNFI6KM7:TR?= M2jnbXNCVkeHUh?=
LoVo NXPORlNOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUTJR|UxRTBwMkCyNFIh|ryP NFPrcGFUSU6JRWK=
HAL-01 NYqxNY5OT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVfJR|UxRTBwMkCyPVch|ryP MlHaV2FPT0WU
HCC2218 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF7MTmZKSzVyPUCuNlA3QDhizszN MYTTRW5ITVJ?
BEN M4i4Rmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVLJR|UxRTBwMkGyPVMh|ryP MkC5V2FPT0WU
LC-2-ad NWm0VVl[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4K1UWlEPTB;MD6yNlA5PCEQvF2= NVXwNnFwW0GQR1XS
LXF-289 NYHpWYNrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXLJR|UxRTBwMkWwOFEh|ryP M4LUS3NCVkeHUh?=
HGC-27 NEW3UINIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MorTTWM2OD1yLkK2OFE1KM7:TR?= Mn\yV2FPT0WU
LK-2 M4jremdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGq3XJdKSzVyPUCuNlY5OjRizszN MlPtV2FPT0WU
CTB-1 NIrlUWtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2LlNmlEPTB;MD6yO|IyPSEQvF2= Ml7JV2FPT0WU
697 M4T4SWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4X4SGlEPTB;MD6yO|M3PyEQvF2= M3XDfnNCVkeHUh?=
ES7 NI[3PIZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mmr4TWM2OD1yLkK3N|g{KM7:TR?= MX\TRW5ITVJ?
MG-63 NFu0b|VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4XZdmlEPTB;MD6yO|QxPyEQvF2= NEnScXFUSU6JRWK=
T47D NWPhVXpRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEfPVWFKSzVyPUCuNlc1PzVizszN NXzqWYJSW0GQR1XS
RPMI-8226 NWLjVGFHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFnLNplKSzVyPUCuNlg2QDlizszN NF3hTFZUSU6JRWK=
NCI-H292 M3vQT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2XOPGlEPTB;MD6yPVE3PCEQvF2= MUXTRW5ITVJ?
HT MojpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MortTWM2OD1yLkK5N|gh|ryP MYfTRW5ITVJ?
A3-KAW NF64T21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn:5TWM2OD1yLkK5O|Qh|ryP MkDXV2FPT0WU
LB2241-RCC MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoLFTWM2OD1yLkOwNVk5KM7:TR?= Ml\BV2FPT0WU
BB65-RCC Mn35S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFmyfYZKSzVyPUCuN|A5ODVizszN M3O0cnNCVkeHUh?=
G-401 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXrJXWxZUUN3ME2wMlMxQTh4IN88US=> MWXTRW5ITVJ?
TYK-nu NY[xeI9oT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEfJb2lKSzVyPUCuN|E5PjRizszN NYXXZml{W0GQR1XS
NCI-SNU-1 NGTtbY5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXXPdJpsUUN3ME2wMlMzOzNzIN88US=> Mo\XV2FPT0WU
CHL-1 M2Htdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1\lemlEPTB;MD6zNlcxOyEQvF2= NXnGO2JlW0GQR1XS
P30-OHK M2TRRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWr0e4J{UUN3ME2wMlMzPzB3IN88US=> MWXTRW5ITVJ?
PA-1 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWPhN|ZqUUN3ME2wMlM{PjF7IN88US=> NUDrfnV2W0GQR1XS
LB1047-RCC NWnj[pA1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4HFdmlEPTB;MD6zOFA1PyEQvF2= MljGV2FPT0WU
EM-2 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWDGOo5XUUN3ME2wMlM1OTh6IN88US=> NIXK[5JUSU6JRWK=
VA-ES-BJ MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVP4ZVhPUUN3ME2wMlM1OjZ6IN88US=> NGnDb2VUSU6JRWK=
NCI-H520 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFfxUINKSzVyPUCuN|Q5OzRizszN NYLETIVXW0GQR1XS
ECC10 NHHSfHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV;JR|UxRTBwM{WwN|Uh|ryP NUCxW2VEW0GQR1XS
LNCaP-Clone-FGC MkXRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXHBdo4zUUN3ME2wMlM2OzF2IN88US=> NV22VFZ6W0GQR1XS
ES8 M{j0Nmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXqycm5qUUN3ME2wMlM3PTV|IN88US=> MnnlV2FPT0WU
HuO-3N1 MkjrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXnJR|UxRTBwM{izO|kh|ryP MVnTRW5ITVJ?
L-363 NIG1Vo5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWC2ZppMUUN3ME2wMlM6ODJ4IN88US=> NHHSOGtUSU6JRWK=
SK-LU-1 NXrNcmRPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml34TWM2OD1yLkO5NlA5KM7:TR?= NI[4dYFUSU6JRWK=
MCF7 NHTGWmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVvQVYZWUUN3ME2wMlQxPTNizszN MWrTRW5ITVJ?
HuO9 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmLNTWM2OD1yLkSwOlY4KM7:TR?= NHzvOmhUSU6JRWK=
BHT-101 NULrXo55T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXfN[YZuUUN3ME2wMlQxPzh5IN88US=> M4rTc3NCVkeHUh?=
MZ2-MEL MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3fSWGlEPTB;MD60NFg5OiEQvF2= NF[3dIJUSU6JRWK=
NOS-1 M{DXemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoLFTWM2OD1yLkSxNFc1KM7:TR?= NIK5TolUSU6JRWK=
NEC8 M1TFc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVfJR|UxRTBwNEG2PVUh|ryP MXLTRW5ITVJ?
COR-L88 NGXhbmVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIrmUYpKSzVyPUCuOFIzPjdizszN M3rxbnNCVkeHUh?=
MFM-223 NHuzcmhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlfrTWM2OD1yLkSyOlE4KM7:TR?= M2S4[3NCVkeHUh?=
HSC-3 NFvsXYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXLJR|UxRTBwNEK5NFgh|ryP NWDHOXVHW0GQR1XS
OVCAR-5 M{n3UWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWnJR|UxRTBwNEK5N|Mh|ryP NVjudlBiW0GQR1XS
MFE-280 M{L1emdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml7MTWM2OD1yLkSzNFMzKM7:TR?= NVfHcZpyW0GQR1XS
KP-N-YS MnrWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmHsTWM2OD1yLkSzNFch|ryP NGfQRXFUSU6JRWK=
EPLC-272H NGj4[25Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkfCTWM2OD1yLkSzNFc4KM7:TR?= MYDTRW5ITVJ?
KE-37 NV3OUINpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3XxU2lEPTB;MD60N|I1PyEQvF2= MU\TRW5ITVJ?
HuH-7 NH60bZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3u1V2lEPTB;MD60N|M4OiEQvF2= NYrHZmh3W0GQR1XS
CHP-212 NWPPNYdsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWDJR|UxRTBwNEO2PVch|ryP M3fBUHNCVkeHUh?=
SK-MES-1 NH3re3NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHPBNpRKSzVyPUCuOFM5PDhizszN M1nYUnNCVkeHUh?=
SJSA-1 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXXPU2VwUUN3ME2wMlQ1PTV2IN88US=> NVeyWGhiW0GQR1XS
QIMR-WIL NUTqXmY4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{XZZ2lEPTB;MD60OVE2KM7:TR?= NVXqOHA2W0GQR1XS
ES4 MkTtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MojSTWM2OD1yLkS1N|M3KM7:TR?= MnT3V2FPT0WU
TI-73 NV20[4dDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEHYTopKSzVyPUCuOFU3QDZizszN M17TTXNCVkeHUh?=
COLO-684 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXjaR216UUN3ME2wMlQ2PzJ4IN88US=> MmnqV2FPT0WU
GCIY MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXTEdHNDUUN3ME2wMlQ2PzV2IN88US=> M4CwPHNCVkeHUh?=
JVM-3 MoPSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWjHVYJbUUN3ME2wMlQ3Ojl{IN88US=> MY\TRW5ITVJ?
MPP-89 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml32TWM2OD1yLkS2PVQ4KM7:TR?= MlXxV2FPT0WU
M14 M33MOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkD6TWM2OD1yLkS3NVch|ryP NF\FRnFUSU6JRWK=
SW962 NUPTeVRZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIDCb4FKSzVyPUCuOFcyQSEQvF2= M2\QR3NCVkeHUh?=
VM-CUB-1 Mn:2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYDEfnpHUUN3ME2wMlQ4Ojh4IN88US=> NXvUeGJKW0GQR1XS
A101D NHHwVpdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MW\JR|UxRTBwNEi5Olgh|ryP MonaV2FPT0WU
DB NYq5SmFwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGr4WY1KSzVyPUCuOVAxOjFizszN MkKzV2FPT0WU
KNS-62 NVy5T2x1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGX6[5hKSzVyPUCuOVA2PjhizszN NG\ifFhUSU6JRWK=
NCI-H460 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWjqe49rUUN3ME2wMlUxPTl5IN88US=> M2XDfXNCVkeHUh?=
MDA-MB-361 M3\hbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2P6cmlEPTB;MD61NFg4KM7:TR?= NUP6eXUzW0GQR1XS
DOK MnrUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUDhWFFTUUN3ME2wMlUyOzN|IN88US=> NInLc|BUSU6JRWK=
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8-MG-BA MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYDJR|UxRTFwOUiyNUDPxE1? NVfLfpNHW0GQR1XS
OVCAR-8 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NG\POGtKSzVyPUKuNFAzQTVizszN NY\GVlcxW0GQR1XS
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RCC10RGB NWLWWJdIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoX6TWM2OD1{LkC0PFcyKM7:TR?= MoDJV2FPT0WU
SW954 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmjtTWM2OD1{LkC1NFM3KM7:TR?= NFTISpRUSU6JRWK=
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CAS-1 NUXuSmNlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3zHPGlEPTB;Mj6xNVU1PiEQvF2= NV[2TGt3W0GQR1XS
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HT-1376 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXjJR|UxRTJwM{i3NFgh|ryP MkXYV2FPT0WU
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NB10 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYTO[llxUUN3ME2yMlQ6PDJ7IN88US=> M4H0c3NCVkeHUh?=
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COLO-679 Mn3QS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NE\DRpZKSzVyPUKuOlEzOzNizszN MUPTRW5ITVJ?
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EW-13 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml74TWM2OD1{Lk[4O|Y5KM7:TR?= M4nidHNCVkeHUh?=
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NCI-H810 M2PqXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{G0VGlEPTB;Mj63NlE6QCEQvF2= M4P0XnNCVkeHUh?=
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NCI-N87 NVPtRmpHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXH4d4VQUUN3ME2yMlkyQDB5IN88US=> NYi0d2dPW0GQR1XS
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NCI-H2170 NFnlPXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWjS[GF4UUN3ME2zMlAxPDVzIN88US=> MoTiV2FPT0WU
OVCAR-3 MmLJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH3IVYZKSzVyPUOuNFIyOTVizszN NWTuOm9VW0GQR1XS
KLE M{izXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn7mTWM2OD1|LkC2PFEzKM7:TR?= MXfTRW5ITVJ?
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MC116 NWT6XHNqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYfMVGVoUUN3ME2zMlEyOTF{IN88US=> MXLTRW5ITVJ?
SCC-9 M3nVbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUDkWJRoUUN3ME2zMlEyOzZ4IN88US=> NF7D[5pUSU6JRWK=
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U-266 M3zr[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX36TWhVUUN3ME2zMlI4PjN|IN88US=> NETLWmVUSU6JRWK=
JVM-2 NIHXdYFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUDx[oY{UUN3ME2zMlMzOTZ2IN88US=> M1\YbXNCVkeHUh?=
UACC-257 NVfJelJDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NELGPFhKSzVyPUOuOFY1OzFizszN MUXTRW5ITVJ?
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BT-549 M{jIRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmXUTWM2OD1|LkWwNlA6KM7:TR?= M37vWHNCVkeHUh?=
NCI-H526 NEe4Z2FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXTJR|UxRTNwNUSxOVgh|ryP NIjpZoFUSU6JRWK=
RH-18 NEXaWIdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml36TWM2OD1|Lk[xO|k5KM7:TR?= MkfvV2FPT0WU
SW626 NWLkfVE5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWC1V4RMUUN3ME2zMlcyOTJ6IN88US=> MV\TRW5ITVJ?
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IGR-1 NILweWNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXvJR|UxRTNwN{SyOFgh|ryP MlO2V2FPT0WU
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NCI-H441 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4XC[GlEPTB;Mz64NlQ{OyEQvF2= NXPUVmh2W0GQR1XS
D-247MG NEjFeJZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVnlNXloUUN3ME2zMlg3ODNzIN88US=> MWPTRW5ITVJ?
COLO-320-HSR NFji[IJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmT1TWM2OD1|LkmxNlcyKM7:TR?= NXfoZ5d1W0GQR1XS
A253 M4j1Rmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3POWmlEPTB;Mz65NVQ{PiEQvF2= M3TzWnNCVkeHUh?=
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MZ1-PC MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3W4TGlEPTB;Mz65OVg3PCEQvF2= M1riXXNCVkeHUh?=
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SF268 MmS5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYTVZ4hMUUN3ME20MlEzQDhzIN88US=> NIHRNVhUSU6JRWK=
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CFPAC-1 NWnRfJNIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWrvOGFxUUN3ME20MlIyPjh3IN88US=> MWPTRW5ITVJ?
S-117 NVPKOpJST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnfSTWM2OD12LkKyNFI1KM7:TR?= NH\UcXBUSU6JRWK=
NCI-H2122 M1rYT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1PQcmlEPTB;ND6zNFk{OSEQvF2= M2DTUHNCVkeHUh?=
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CAL-72 M2jaXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEDnb5ZKSzVyPUSuN|U1PzdizszN M{Tq[HNCVkeHUh?=
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UACC-62 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1vrcGlEPTB;ND60OlU1PSEQvF2= NIHhfGVUSU6JRWK=
MS-1 NVHafY16T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWnYVFFSUUN3ME20MlQ6OTF3IN88US=> M2nF[HNCVkeHUh?=
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DK-MG M1vxW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoHQTWM2OD12Lke4NVEzKM7:TR?= Mn;EV2FPT0WU
HCC1937 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MU\JR|UxRTRwOEWxNlUh|ryP Mk\VV2FPT0WU
OAW-28 M{fLe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX;JR|UxRTRwOUK0N|Uh|ryP NELycmxUSU6JRWK=
MEG-01 MkDLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXLDfZZNUUN3ME21MlA5OzJ6IN88US=> NXPGNmxoW0GQR1XS
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FADU MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVrJR|UxRTVwMke4N|ch|ryP NUXifolUW0GQR1XS
CAPAN-1 M4TNW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3m2SWlEPTB;NT6zPVQ{OiEQvF2= M{PlU3NCVkeHUh?=
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DBTRG-05MG MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1jOcWlEPTB;NT61NFUzKM7:TR?= NXjNR4M{W0GQR1XS
ONS-76 NV;YSplMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmrlTWM2OD13LkW1PFAyKM7:TR?= M1XoUnNCVkeHUh?=
Ca-Ski M2LmPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkW1TWM2OD13Lk[yOVY3KM7:TR?= MmLoV2FPT0WU
COLO-741 NHrKZ4VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1roSGlEPTB;NT63NVk6PSEQvF2= MV\TRW5ITVJ?
HT-29 NVe2SIlZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlfMTWM2OD13LkiwOFc2KM7:TR?= MWDTRW5ITVJ?
HCC1187 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWjETWZ4UUN3ME21MlgyODB6IN88US=> M2H5OXNCVkeHUh?=
Capan-2 MnK1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mmj2TWM2OD13Lkm1PVk1KM7:TR?= NGj5UlRUSU6JRWK=
NCI-H630 NITpOJBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVXJR|UxRTZwMUG0OFEh|ryP NIHxd|VUSU6JRWK=
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D-502MG NUe3XZhwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUS2PWk6UUN3ME22MlE4OzN4IN88US=> MmSxV2FPT0WU
NB17 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkjQTWM2OD14LkK0PVQ{KM7:TR?= NFPZcFFUSU6JRWK=
SK-MEL-24 NHjIU|BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUfJR|UxRTZwMk[0PUDPxE1? M1nVW3NCVkeHUh?=
NCI-SNU-5 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{jyZmlEPTB;Nj6yO|EyQCEQvF2= Mn3DV2FPT0WU
D-392MG MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXPJR|UxRTZwM{OzPVkh|ryP NV:wdYFVW0GQR1XS
A498 MnHRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{TYbGlEPTB;Nj60NFMxOiEQvF2= MoPKV2FPT0WU
OCI-AML2 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml;qTWM2OD14LkS1OFQ2KM7:TR?= M4C4UXNCVkeHUh?=
HCC2157 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mon0TWM2OD14LkS1OlE3KM7:TR?= MnHtV2FPT0WU
C3A M{DJOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVLvNo01UUN3ME22MlUzODB2IN88US=> M1PiZ3NCVkeHUh?=
NCI-H1650 NYfi[2ZKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3nVTWlEPTB;Nj63NFUzOiEQvF2= M2O4TnNCVkeHUh?=
DU-145 MmHBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoHaTWM2OD14Lke3NFMh|ryP M{nuT3NCVkeHUh?=
COLO-829 NWDXSZhUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoDyTWM2OD14Lke3PFc{KM7:TR?= MVvTRW5ITVJ?
LS-1034 NUnUZY9ET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2H1emlEPTB;Nz6wOFQyOiEQvF2= NXPWZWFjW0GQR1XS
HCC1954 MlX6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2PyemlEPTB;Nz60PFE{PCEQvF2= NX7qc2p[W0GQR1XS
KURAMOCHI NWLaUVBLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M37MXWlEPTB;Nz60PFU{OyEQvF2= MmPaV2FPT0WU
Mo-T MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2DrcmlEPTB;Nz61NVQ6QCEQvF2= Mo\IV2FPT0WU
OE19 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWTJR|UxRTdwNkW1NFMh|ryP MWnTRW5ITVJ?
COR-L105 NG\nRVhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVvXZ2tpUUN3ME23MlcyPDJzIN88US=> MkPKV2FPT0WU
NCI-H2342 NYX2U4czT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV\GOmlLUUN3ME23MlcyPTJzIN88US=> M{ewfHNCVkeHUh?=
U-87-MG MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2nqNmlEPTB;OD6wN|M{PCEQvF2= NGW1VmRUSU6JRWK=
MKN28 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1O5bGlEPTB;OD6xNVUyPSEQvF2= NFHFPIVUSU6JRWK=
HT-1197 NFf1VnpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXrJR|UxRThwMkW5NFch|ryP NHrlb5BUSU6JRWK=
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NCI-H226 Mo[0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVvJR|UxRThwNESzN|Eh|ryP NYPiRod7W0GQR1XS
NCI-H2029 NEPRb2VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4L5eGlEPTB;OT6wOlc2QSEQvF2= M1znXnNCVkeHUh?=
TE-11 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEPlPJhKSzVyPUmuOFU2QDhizszN NUC1cHc1W0GQR1XS
ZR-75-30 NVzXTmRKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYLJR|UxRTlwOUK2N|Qh|ryP NXzET2tKW0GQR1XS
SW837 NUf6Z5NrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmfuTWM2OD1zMD6yNlk{KM7:TR?= MmrtV2FPT0WU
NCI-H1651 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3\LVGlEPTB;MUCuNlMzPiEQvF2= M{i5WHNCVkeHUh?=
NCI-H727 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3\EN2lEPTB;MUCuOFQ6PSEQvF2= NEDrbVNUSU6JRWK=
NCI-H358 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGDNT3VKSzVyPUGwMlg{PjVizszN Mmi0V2FPT0WU
NCI-H2087 MlLKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUHkepNzUUN3ME2xNU4xPDN2IN88US=> MWHTRW5ITVJ?
FTC-133 M4XJRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlPZTWM2OD1zMT60NFUyKM7:TR?= MVrTRW5ITVJ?
LAN-6 NF7sOYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEnvS2RKSzVyPUGyMlEzPCEQvF2= MkfhV2FPT0WU
NCI-H1838 M4ToOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1Hyb2lEPTB;MUKuOVE2OSEQvF2= MnfPV2FPT0WU
Calu-6 M{eycGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoPLTWM2OD1zND6xNFIyKM7:TR?= NYPoVoJxW0GQR1XS
HCC70 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUjJR|UxRTF2LkS1NUDPxE1? NE\jboFUSU6JRWK=
KARPAS-299 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MW\JR|UxRTF3LkKxOVkh|ryP NFTHeJVUSU6JRWK=
CAL-27 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHTpO4xKSzVyPUG1MlM5PDNizszN MWTTRW5ITVJ?
NCI-H82 MmTNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmjxTWM2OD1zNT61N|Ih|ryP M3q2UXNCVkeHUh?=
no-10 M3XQPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIDNZlJKSzVyPUG1Mlk{QCEQvF2= MY\TRW5ITVJ?
U251 NGLRRnJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWHJR|UxRTF4LkO2O|Uh|ryP MnHnV2FPT0WU
TE-9 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFvON25KSzVyPUG3MlUyPjZizszN MmryV2FPT0WU
TE-10 M4LqRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXLOZ2NTUUN3ME2xPU4xPjB5IN88US=> MVXTRW5ITVJ?
COLO-824 NHixU|BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkCzTWM2OD1{MD65Nlc3KM7:TR?= MVvTRW5ITVJ?
HuP-T3 Ml7XS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{HadmlEPTB;MkGuNFc1QCEQvF2= NWTIVZU2W0GQR1XS
KALS-1 NFSwOnRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXPJR|UxRTJzLkG3OUDPxE1? MmHXV2FPT0WU
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ABC-1 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2XrbmlEPTB;Mk[uO|k2PyEQvF2= NH[wcoRUSU6JRWK=
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NY M2\NbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVXJR|UxRTRzLkOyNFEh|ryP M2PUUnNCVkeHUh?=
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MCF7-X  NHG2TlJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWPJR|UxRTJ2IH7N M{jmfVI1PDV5ME[5
T47D-tamR  NGLiTI1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGLEPZBKSzVyPUG5JI5O MojJNlQ1PTdyNkm=
293T  NIPWdI5HfW6ldHnvckBCe3OjeR?= Mmi2NlAhdk1? M{TLSFMhcA>? MorD[Il{enWydIOgRYt1NW2nZHnheIVlKFSLRj3JRUB{fGGkaXzpeJktKHS{YX7zcI9k[XSrb36sJIFv\CCjY4Tpeol1gQ>? M3fnbFI1OzZ|NES5
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Huh7  M3G2WWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIDrUWc2NTFyMDDuUS=> NX\DbIVoOC1{NDDo M2LMbmROW09? MVLpcoNz\WG|ZYOgZ4VtdCCmZXH0bEBqdiCjIHPvcoNmdnS{YYTpc44uKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI> MkDDNlQzQTd|MEC=
Hep3B  NVK2UI5jT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUG1SpF6PS1zMECgcm0> NGG2WIMxNTJ2IHi= MUPEUXNQ MVzpcoNz\WG|ZYOgZ4VtdCCmZXH0bEBqdiCjIHPvcoNmdnS{YYTpc44uKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI> MYqyOFI6PzNyMB?=
Huh7  M1G1fGZ2dmO2aX;uJGF{e2G7 NHLPZW8yODBibl2= MWSxNkBp NE\YdZdFVVOR NXHsV5lIcW6mdXPld{BifXSxcHjh[5nDqA>? NHLLUmIzPDJ7N{OwNC=>
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Hep-2 NWXodWg{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFrMfVExNjBzLUGg{txO NEfCNVAyOi17NjDo MULpcoNz\WG|ZYOgZ4VtdCCmZXH0bEBqdiCjIHPvcoNmdnS{YYTpc44uKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI> MVqyOFExOzd2OR?=
SCC-9 M1TBPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFjEW3oxNjBzLUGg{txO NWnkSnpsOTJvOU[gbC=> M2PsV4lv[3KnYYPld{Bk\WyuIHTlZZRpKGmwIHGgZ49v[2WwdILheIlwdi1iYX7kJJRqdWVvZHXw[Y5l\W62IH3hco5meg>? MYqyOFExOzd2OR?=
Hep-2 MUPGeY5kfGmxbjDBd5NigQ>? NI\CUpIxNjFxMD61JO69VQ>? NYDIZ2JPOjRiaB?= NXrmdY9y[myxY3vzJI1VV1KFMTDhcoQhdVSRUlOyJIFkfGm4YYTpc44> NYTi[VBROjRzMEO3OFk>
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PANC-1 M1fxXWFxd3C2b4Ppd{BCe3OjeR?= MUC1NFAhdk1? NV\Yb2lMPDhiaB?= NF3lO3V{\W6|aYTpfoV{KHCjbnPy[YF1cWNiY3HuZ4VzKGOnbHzzJJRwKHKjZHnveIhmemGyeR?= MVmyN|g5PjJ7NB?=
RMG2 NFe2fJVCeG:ydH;zbZMhSXO|YYm= NH:4VVgyNTFyMECgcm0> NVzuTmw3PzJiaB?= NFTUUVZHSlN? Mn\6bY5pcWKrdIOgeIhmKGOnbHygeoli[mmuaYT5JINwdmOnboTyZZRqd25iZHXw[Y5l\W62bIm= MY[yN|YyPTZ|MR?=
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HT-29 MYfBdI9xfG:|aYOgRZN{[Xl? MUSxJO69VQ>? NU[xVG12PzJiaB?= MWrheJRmdnWjdHXzJFUuTlVvaX7keYNm\CClZXzsJIRm[XSq MUCyNVk1QTF{MR?=
DLD1 NH;ze3VCeG:ydH;zbZMhSXO|YYm= NV3rXXdSOSEQvF2= M{W3[VczKGh? M1TFdoF1fGWwdXH0[ZMhPS2IVT3pcoR2[2WmIHPlcIwh\GWjdHi= Mm\CNlE6PDlzMkG=
HT-29 NUfnV|BjTnWwY4Tpc44hSXO|YYm= MYCxJO69VQ>? NYPCOIhmPzJiaB?= NVXNUFN6cW6mdXPld{BifXSxcHjh[5khfG9iY3;u[oVzKGO7dH;wdo91\WO2aX;u M1nHSlIyQTR7MUKx
DLD1 M{fNPGZ2dmO2aX;uJGF{e2G7 MUWxJO69VQ>? MoTjO|IhcA>? MkTjbY5lfWOnczDheZRweGijZ4mgeI8h[2:wZnXyJIN6fG:ycn;0[YN1cW:w NUXOfohJOjF7NEmxNlE>
Rh41 NHLZc21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4X5cGlEPTB;M{CuO{BvVQ>? NWfyeIZTOjF|M{e2O|k>
Rh18 NVjrNXBpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn;GTWM2OD12MT60JI5O Mlz5NlE{Ozd4N{m=
Rh30 NXq2PXQ3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3va[mlEPTB;MUSgcm0> NHrPdIIzOTN|N{[3PS=>
BT-12 NH60Um1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{S5ZmlEPTB;MkSuO{BvVQ>? NWX0PVlpOjF|M{e2O|k>
CHLA-266 M2XaW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkPqTWM2OD1zLkGgcm0> NF6xSVczOTN|N{[3PS=>
TC-71 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGGzWoVKSzVyPUS2MlUhdk1? MkPENlE{Ozd4N{m=
CHLA-9 NX7xSnFmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoTETWM2OD1zNT6zJI5O NXjTcJZ4OjF|M{e2O|k>
CHLA-10 Mn65S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3jLc2lEPTB;Mz65JI5O M1fX[FIyOzN5Nke5
CHLA-258 NGHEVGhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUnJR|UxRTFyLkSgcm0> MVuyNVM{PzZ5OR?=
GBM2 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV3JR|UxRTRyLkegcm0> MWmyNVM{PzZ5OR?=
NB-1643 NYfqS4U5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{PNSGlEPTB;M{OuPEBvVQ>? NITE[lkzOTN|N{[3PS=>
NB-EBc1 MlLrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEDK[G1KSzVyPUK3MlEhdk1? MmP4NlE{Ozd4N{m=
CHLA-90 Ml\nS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWrJR|UxRTN2LkWgcm0> NWG2S5JvOjF|M{e2O|k>
CHLA-136 M3vGRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUDJR|UxRTF4Mj62JI5O NVLyd|FQOjF|M{e2O|k>
NALM-6 M3PIO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF;sR|FKSzVyPUOyMlkhdk1? NI\NRYozOTN|N{[3PS=>
COG-LL-317 NIPsdVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUDJR|UxRTFwMzDuUS=> MWeyNVM{PzZ5OR?=
RS4;11 NVTHenlTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MorHTWM2OD1|NTDuUS=> MkPjNlE{Ozd4N{m=
MOLT-4 NXLrbZVPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHzDPIpKSzVyPUiuOkBvVQ>? NFi1cVczOTN|N{[3PS=>
CCRF-CEM NGnLO2NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NETDeJhKSzVyPUSuNUBvVQ>? MkOxNlE{Ozd4N{m=
Kasumi-1 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NI\2dmpKSzVyPUGuOEBvVQ>? MmH3NlE{Ozd4N{m=
Karpas-299 NITJR3lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MonXTWM2OD1{MT63JI5O M1vRU|IyOzN5Nke5
Ramos-RA1 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYD0NmpPUUN3ME24MlIhdk1? NYPxTWRZOjF|M{e2O|k>
H838 M2SzUGZ2dmO2aX;uJGF{e2G7 NH:xOnMyKM7:TR?= M1XFVlQ5KGh? NXKwfXJZcW6mdXPld{Bk\WyuIHH1eI9xcGGpeR?= M3vXfFIxOzZ2MUGz
H838 MmTUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MonYNE0{KM7:TR?= M2PFc|czKGh? MW\pcoR2[2W|IHfyc5d1cCCrbnjpZol1cW:wIHHu[EBifXSxcHjh[5k> M{DBUFIxODJ6OEW0
A549  MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mme1NE0{KM7:TR?= NGnYPXA4OiCq M4f4[Ilv\HWlZYOg[5Jwf3SqIHnubIljcXSrb36gZY5lKGG3dH;wbIFogQ>? NUHSOWQxOjByMki4OVQ>

... Click to View More Cell Line Experimental Data

In vivo AZD8055 inhibits the pS6 and pAkt in U87MG and A549 xenografts at 2.5/10 mg/kg, which leads to tumor growth inhibition. AZD8055 shows significant antitumor activity in many xenografts, including U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA at a dose of 10/20 mg/kg. [1] AZD8055 induces ~40% reduction in tumour volume, accompanied by ablation of phosphorylation of Akt, S6K and SGK protein kinases. Administration of AZD8055 (5mg/kg, Bid) and SAHA (100 mg/kg/d) results in complete tumor growth inhibition in PTEN+/−LKB1+/hypo xenografts without side effects on mice by inhibition of mTORC1 and mTORC2 signaling. [4]

Protocol

Kinase Assay:[1]
+ Expand

Cell-based determinations of mTOR inhibition:

A high-throughput screening cell-based assay is developed using MDA-MB-468 cells to detect mTORC1 and mTORC2 activity. Cells are exposed for 2 hours to increasing concentrations of AZD8055. At the end of the incubation period, cells are fixed, washed, and probed with antibodies against S473 pAkt or against S235/236 phosphorylated S6 (pS6). Levels of phosphorylation are assessed using an Acumen laser scanning cytometer.
Cell Research:[1]
+ Expand
  • Cell lines: U87MG, A549 and H838 cells
  • Concentrations: 1 nM - 1 μM
  • Incubation Time: 72 to 96 hours
  • Method: Cells are exposed to AZD8055 for 72 to 96 hours and stained for cell nuclei (0.03 mg/mL Hoechst 33342) and acidic vesicles (1 μg/mL acridine orange). Images are captured at 450 and 536 nm on an ArrayScan II platform, and the percentage of acidic vesicles and the number of cells are quantified. For LC3 assessment, cells are exposed to e64d/pepstatin (10 μg/mL) for 30 to 90 min before incubation with AZD8055. Cells are lysed on ice and analyzed by immunoblotting.
    (Only for Reference)
Animal Research:[1]
+ Expand
  • Animal Models: U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA U87-MG and A549 are established in pathogen-free, female nude mice (nu/nu:Alpk).
  • Formulation: Dissolved in captisol (CyDex, pH 3.0) and diluted to a final captisol concentration of 30% (w/v)
  • Dosages: 2.5-20 mg/kg
  • Administration: Oral gavage once or twice daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 50 mg/mL warmed (107.4 mM)
Ethanol 3 mg/mL (6.44 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order:
4% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 465.54
Formula

C25H31N5O4

CAS No. 1009298-09-2
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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    C2
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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01316809 Completed Glioblastoma Multiforme|Anaplastic Astrocytoma|Anaplastic Oligodendroglioma|Malignant Glioma|Brainstem Glioma National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) March 4, 2011 Phase 1
NCT00999882 Completed Cancer|Advanced Hepatocellular Carcinoma AstraZeneca October 2009 Phase 1
NCT00973076 Completed Cancer|Solid Tumors|Advanced Solid Malignancies AstraZeneca August 2009 Phase 1
NCT00731263 Completed Solid Tumors AstraZeneca July 2008 Phase 1
NCT01194193 Withdrawn Cancer|Advanced Solid Tumours|Lymphomas AstraZeneca null Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID