AZD8055

Catalog No.S1555

AZD8055 Chemical Structure

Molecular Weight(MW): 465.54

AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. Phase 1.

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In DMSO USD 156 In stock
USD 120 In stock
USD 370 In stock
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Cited by 38 Publications

8 Customer Reviews

  • PC3-LN4 cells were treated with GSK690693 (5 uM) alone, or in combination with PP242 (2 uM) or AZD8055 (1 uM) for 24 h, and immunoblotting performed.

    Cancer Res 2013 73(11), 3402-11. AZD8055 purchased from Selleck.

    HUVEC were treated with either RAD001 or AZD8055 before PA treatment. p53, GPX1, p53 acetylation, and subcellular PTEN were analyzed with corresponding antibodies. The blot is a representative of the results of three experiments. Values (mean盨D) are expressed as fold over untreated control. *p<0.05 versus their corresponding control groups in the one-way ANOVA.

    Antioxid Redox Signal 2014 20(9), 1382-95. AZD8055 purchased from Selleck.

  • (A) HCT116 or MDA-MB-468 cells were treated with 500 nM AZD8055 for the indicated times. Cell lysates were immunoblotted with the indicated antibodies. (B) HCT116 or MDA-MB-468 cells were treated with 50 nM rapamycin. 500 nM AZD8055, 1 uM MK2206 or dimethyl sulfoxide (DMSO) as control for 12 h. Cell lysates were immunoblotted with the indicated antibodies.

    Oncotarget 2014 5(15), 6015-27. AZD8055 purchased from Selleck.

    CSCs+24/44/ESA at 5,000 cells per well with stem cell culture media in triplicate in an ultra-low adhesion plate were treated with 5 umol/L BMS-777607, 1 umol/L AZD8055 alone, or in their different combinations. Cells were cultured for 72 hours. Cell viability was determined by the MTS assay. Percentages of polyploid cells were determined by counting 300 cells from two different regions. Results shown here were from one of two experiments with similar results.

    Mol Cancer Ther 2014 13(1), 37-48. AZD8055 purchased from Selleck.

  • mTOR kinase inhibitor AZD8055 activates PI3K accompanied with induction of expression of EGFR, HER2, HER3 and IRS1. Serum-deprived CHO-EGFP-AKT cells were incubated with 50 nM AZD8055 for 24 hr. The EGFP signal was detected using confocal microscopy. The white arrows indicate EGFP-AKT located on cellular membrane.

    Int J Cancer 2014 135(10), 2462-74. AZD8055 purchased from Selleck.

    mTOR, P70S6K1, 4E-BP1 and PCNA activation in the basilar artery of SAH. Top, Western blotting bands of antibodies, bottom, density of the protein band from each group. A, mTOR activation in the basilar artery demonstrated an elevated expression of mTOR on day 7 following SAH in SAH and SAH+DMSO animals. The mTOR inhibitors, Rapamycin and AZD8055 reduced the enhanced expression of mTOR (pb0.05, ANOVA). B. P70S6K1 activation. C. 4E-BP1 activation. D. PCNA activation. Rapamycin and AZD8055 significantly decreased the expression of P70S6K1, 4E-BP1 and PCNA compared with the SAH and SAH+DMSO (pb0.05, respectively; ANOVA). There was no significant difference between the two inhibitors in mTOR, P70S6K1, 4E-BP1 and PCNA expression.

    Exp Neurol 2012 233, 799-806. AZD8055 purchased from Selleck.

  • Induction of TCTP expression through 5-FU or oxaliplatin is inhibited by mTOR kinase inhibitors. HCT116 cells were treated for 48 h with 5-FU or oxaliplatin at the indicated concentrations. Where indicated, AZD 8055 (100 nM) or PP242 (1 μM) were added. TCTP levels were assessed by western blotting; signals were quantified and normalised against α-tubulin as a loading control. As a readout of mTOR activity, phosphorylation of the ribosomal protein S6 at Ser 240/244 was assessed using a phospho-specific antibody. a. Representative western blots demonstrating the alteration of the levels of TCTP protein and of phospho-ribosomal protein S6, respectively, in relation to the drug concentration.

    Cell Commun Signal, 2017.. AZD8055 purchased from Selleck.

    After starved in serum-free medium for 24h , A549 cells incubated with the indicated concentrations of AZD8055 for 3h , followed by 20-minute stimolation of 100ng/ml EGF

     
     

     

    Dr. Zhang of Tianjin Medical University. AZD8055 purchased from Selleck.

Purity & Quality Control

Choose Selective mTOR Inhibitors

Biological Activity

Description AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. Phase 1.
Features First drug to inhibit both types of mTOR protein.
Targets
mTOR (truncated) [1]
(MDA-MB-468 cells)
mTOR (full length) [1]
(MDA-MB-468 cells)
DNA-PK [1]
(MDA-MB-468 cells)
PI3Kδ [1]
(MDA-MB-468 cells)
PI3Kα [1]
(MDA-MB-468 cells)
0.13 nM 0.8 nM 1370 nM 3200 nM 3590 nM
In vitro

AZD8055 shows low activity (∼1,000-fold) against all PI3K isoforms (α, β, γ, δ) and other members of the PI3K-like kinase family (ATM and DNA-PK). AZD8055 inhibits the phosphorylation of mTORC1 (p70S6K and 4E-BP1) as well as phosphorylation of the mTORC2 (Akt) and downstream proteins. The rapamycin-resistant T37/46 phosphorylation sites on 4E-BP1 can be fully inhibited by AZD8055, resulting in significant inhibition of cap-dependent translation. AZD8055 potently inhibits proliferation in U87MG, A549 and H838 cells with IC50 of 53, 50 and 20 nM, respectively. AZD8055 also induces autophagy and increased LC3-II levels in H838 and A549 cells. [1] AZD8055 decreases AML blast cell proliferation and cell cycle progression, reduces the clonogenic growth of leukemic progenitors and induces caspase-dependent apoptosis in leukemic cells but not in normal immature CD34+ cells. [2] AZD8055 indicates inhibitory against the pediatric preclinical testing program (PPTP) cell lines with IC50 of 24.7 nM and induces significant differences in EFS distribution. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LU-139 MmLuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWTFcIJjUUN3ME2wMlAzOzNzIN88US=> Mn\jV2FPT0WU
NCI-H720 NFn0VmhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoLjTWM2OD1yLkCyPVM5KM7:TR?= NE\BZo1USU6JRWK=
KARPAS-45 M{H2XWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUfrTVdwUUN3ME2wMlA{OzdzIN88US=> NH\ySXlUSU6JRWK=
EW-3 NUfXTJdjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVP0PGh4UUN3ME2wMlA1ODV4IN88US=> M33IS3NCVkeHUh?=
SBC-1 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV3adZoyUUN3ME2wMlA1PThzIN88US=> NWDDdlRkW0GQR1XS
NCI-H209 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVfJR|UxRTBwMEW0OVMh|ryP M4XaenNCVkeHUh?=
EoL-1-cell MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF72N5JKSzVyPUCuNFY2PjlizszN NHHM[Y5USU6JRWK=
H9 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUjoUFBzUUN3ME2wMlA3Pjl{IN88US=> NUmzfphLW0GQR1XS
AN3-CA NHzCT2dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFnlcFZKSzVyPUCuNFc6PTFizszN MkjFV2FPT0WU
HH MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWjJR|UxRTBwMEiwNFkh|ryP M2jNbHNCVkeHUh?=
KU812 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYDJR|UxRTBwMEiwOFMh|ryP MkTJV2FPT0WU
DU-4475 NUTGOlNMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIHDWZJKSzVyPUCuNFgxPDdizszN M2XNSXNCVkeHUh?=
CTV-1 MlrVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFKz[JhKSzVyPUCuNFg3PDFizszN MliwV2FPT0WU
BV-173 NXXmUooyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHvRe2NKSzVyPUCuNFkxPjFizszN NITWXlNUSU6JRWK=
ES5 MlGwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml3xTWM2OD1yLkC5N|Uh|ryP M2HlWHNCVkeHUh?=
G-402 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mo\OTWM2OD1yLkC5PVgh|ryP MVLTRW5ITVJ?
MV-4-11 M3\GXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGHlOnRKSzVyPUCuNVA{PDdizszN MVPTRW5ITVJ?
NB69 M3HJXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnfPTWM2OD1yLkGzOUDPxE1? MW\TRW5ITVJ?
EGI-1 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUDJR|UxRTBwMUO1N|Eh|ryP MYPTRW5ITVJ?
EW-18 NYfYNnhrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVnJR|UxRTBwMUSwO|Mh|ryP M1O5d3NCVkeHUh?=
ME-180 NWCwOGk3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{exSmlEPTB;MD6xOFY5OSEQvF2= NHSxXYVUSU6JRWK=
D-283MED M4PKNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MU\JR|UxRTBwMUS3NlMh|ryP MXvTRW5ITVJ?
NB14 M3KxdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4rycmlEPTB;MD6xOlQ1PyEQvF2= NXTZNIxCW0GQR1XS
Ramos-2G6-4C10 NXizNI1KT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFjRSmRKSzVyPUCuNVY6QDNizszN MVjTRW5ITVJ?
NBsusSR MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF7vO4pKSzVyPUCuNVcxOTlizszN NHH1PJdUSU6JRWK=
LAMA-84 MonPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2fa[WlEPTB;MD6xPFA{OiEQvF2= NEnsblZUSU6JRWK=
NCI-H1581 NUi5[25xT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1f2d2lEPTB;MD6xPFk3PiEQvF2= MnLHV2FPT0WU
A204 M{fzc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3zqcWlEPTB;MD6xPFk4KM7:TR?= MYXTRW5ITVJ?
A549 NET6SYdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYDae5lzUUN3ME2wMlE6PTVizszN NX:xfFRrW0GQR1XS
CAL-51 NH\DXZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGLCNJdKSzVyPUCuNVk3PTFizszN MXPTRW5ITVJ?
NKM-1 Mni3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXHBXJBmUUN3ME2wMlIxODJ7IN88US=> M{PsNXNCVkeHUh?=
LoVo MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVTieWpnUUN3ME2wMlIxOjB{IN88US=> MXzTRW5ITVJ?
HAL-01 M1nvSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M37JdmlEPTB;MD6yNFI6PyEQvF2= NYHFVWZoW0GQR1XS
HCC2218 NWWzSoNJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{HMV2lEPTB;MD6yNFY5QCEQvF2= NIrieoNUSU6JRWK=
BEN M3PLc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NY\5R2ZnUUN3ME2wMlIyOjl|IN88US=> NIXWN2dUSU6JRWK=
LC-2-ad M3XpXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX:yeJR{UUN3ME2wMlIzODh2IN88US=> NWDUZ5NTW0GQR1XS
LXF-289 M4H3XWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnraTWM2OD1yLkK1NFQyKM7:TR?= MWPTRW5ITVJ?
HGC-27 NFvqZXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX3JR|UxRTBwMk[0NVQh|ryP MnjwV2FPT0WU
LK-2 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYLJR|UxRTBwMk[4NlQh|ryP MlnqV2FPT0WU
CTB-1 NUPMTo17T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M13EUmlEPTB;MD6yO|IyPSEQvF2= M2PibXNCVkeHUh?=
697 M1TxZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGnD[GlKSzVyPUCuNlc{PjdizszN NHS3N2RUSU6JRWK=
ES7 NVL2[XQyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVzqbnVOUUN3ME2wMlI4Ozh|IN88US=> MXTTRW5ITVJ?
MG-63 NEW5c5ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnzQTWM2OD1yLkK3OFA4KM7:TR?= NYG0OGV3W0GQR1XS
T47D NHXve4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3;SW2lEPTB;MD6yO|Q4PSEQvF2= MmDpV2FPT0WU
RPMI-8226 MmriS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3r3cmlEPTB;MD6yPFU5QSEQvF2= NXXtU3VpW0GQR1XS
NCI-H292 NX;PN|VKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MonXTWM2OD1yLkK5NVY1KM7:TR?= NV3ydm1nW0GQR1XS
HT M3PuW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{LDeWlEPTB;MD6yPVM5KM7:TR?= M2rwZ3NCVkeHUh?=
A3-KAW NWPDSIhCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmjHTWM2OD1yLkK5O|Qh|ryP MmjJV2FPT0WU
LB2241-RCC NE\VWVFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NY\lcoU6UUN3ME2wMlMxOTl6IN88US=> MVXTRW5ITVJ?
BB65-RCC M4TEfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYjJR|UxRTBwM{C4NFUh|ryP NGToWoRUSU6JRWK=
G-401 NULVUWx4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkfiTWM2OD1yLkOwPVg3KM7:TR?= MVzTRW5ITVJ?
TYK-nu MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVzXOppqUUN3ME2wMlMyQDZ2IN88US=> M3r0PXNCVkeHUh?=
NCI-SNU-1 MmrGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M17GTWlEPTB;MD6zNlM{OSEQvF2= M3nlVXNCVkeHUh?=
CHL-1 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV3JR|UxRTBwM{K3NFMh|ryP NHyzdW5USU6JRWK=
P30-OHK NYH6cWFTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH\Nb|VKSzVyPUCuN|I4ODVizszN M2XLWXNCVkeHUh?=
PA-1 NF\EOopIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHvYTpVKSzVyPUCuN|M3OTlizszN NFywV4FUSU6JRWK=
LB1047-RCC MkLPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGe1d5FKSzVyPUCuN|QxPDdizszN NFHyPVNUSU6JRWK=
EM-2 M1LGU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHTuXpNKSzVyPUCuN|QyQDhizszN NW\6T3R2W0GQR1XS
VA-ES-BJ NVLlOWE5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUjmS|RZUUN3ME2wMlM1OjZ6IN88US=> MmrpV2FPT0WU
NCI-H520 M3TNe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV;JR|UxRTBwM{S4N|Qh|ryP NHLsc5pUSU6JRWK=
ECC10 M1PTPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkP0TWM2OD1yLkO1NFM2KM7:TR?= NEnPUJJUSU6JRWK=
LNCaP-Clone-FGC NFLZbXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkHpTWM2OD1yLkO1N|E1KM7:TR?= M37uU3NCVkeHUh?=
ES8 Ml;yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXzPVllVUUN3ME2wMlM3PTV|IN88US=> MnXRV2FPT0WU
HuO-3N1 NF3Lb|ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn22TWM2OD1yLkO4N|c6KM7:TR?= NFy5NphUSU6JRWK=
L-363 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX3UOIhxUUN3ME2wMlM6ODJ4IN88US=> Mm[3V2FPT0WU
SK-LU-1 NGmwbnlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoXmTWM2OD1yLkO5NlA5KM7:TR?= Mn64V2FPT0WU
MCF7 M{LSXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXrJR|UxRTBwNEC1N{DPxE1? M2jQWnNCVkeHUh?=
HuO9 MmHhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkDNTWM2OD1yLkSwOlY4KM7:TR?= NFT4XGJUSU6JRWK=
BHT-101 MoiyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYfiSYNnUUN3ME2wMlQxPzh5IN88US=> NUn2S5hIW0GQR1XS
MZ2-MEL NULWdHJbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH7uOZdKSzVyPUCuOFA5QDJizszN NX2wblhDW0GQR1XS
NOS-1 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWjJR|UxRTBwNEGwO|Qh|ryP MVnTRW5ITVJ?
NEC8 M3LC[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1fuZmlEPTB;MD60NVY6PSEQvF2= M1\iVnNCVkeHUh?=
COR-L88 MonLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVX5TIgxUUN3ME2wMlQzOjZ5IN88US=> MWjTRW5ITVJ?
MFM-223 NYi1RW9VT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1\XVWlEPTB;MD60NlYyPyEQvF2= NGL6UHNUSU6JRWK=
HSC-3 NXfMephCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnHqTWM2OD1yLkSyPVA5KM7:TR?= MoTiV2FPT0WU
OVCAR-5 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUfn[Hh4UUN3ME2wMlQzQTN|IN88US=> NV\tcmFiW0GQR1XS
MFE-280 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX;tdFZCUUN3ME2wMlQ{ODN{IN88US=> M2e1[HNCVkeHUh?=
KP-N-YS MoTiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mk\ZTWM2OD1yLkSzNFch|ryP MWnTRW5ITVJ?
EPLC-272H MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYrJb5dtUUN3ME2wMlQ{ODd5IN88US=> NETrT4lUSU6JRWK=
KE-37 M3rvdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mnn2TWM2OD1yLkSzNlQ4KM7:TR?= M2fUWXNCVkeHUh?=
HuH-7 MkfjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXPibndZUUN3ME2wMlQ{Ozd{IN88US=> NH;0d4tUSU6JRWK=
CHP-212 Mn:2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIPTZZlKSzVyPUCuOFM3QTdizszN M1L2[XNCVkeHUh?=
SK-MES-1 M4TrSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHe2ZYdKSzVyPUCuOFM5PDhizszN MXfTRW5ITVJ?
SJSA-1 NGf1eIJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlnvTWM2OD1yLkS0OVU1KM7:TR?= NFGz[XRUSU6JRWK=
QIMR-WIL NGHFNFJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmiyTWM2OD1yLkS1NVUh|ryP MV\TRW5ITVJ?
ES4 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3qz[GlEPTB;MD60OVM{PiEQvF2= MkLlV2FPT0WU
TI-73 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmnxTWM2OD1yLkS1Olg3KM7:TR?= MoixV2FPT0WU
COLO-684 NG\BTodIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mm\tTWM2OD1yLkS1O|I3KM7:TR?= M2jibHNCVkeHUh?=
GCIY MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWfJR|UxRTBwNEW3OVQh|ryP NG\GUpJUSU6JRWK=
JVM-3 MoXWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1\tZ2lEPTB;MD60OlI6OiEQvF2= MUPTRW5ITVJ?
MPP-89 M{HJdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVrJR|UxRTBwNE[5OFch|ryP NXnYVWtIW0GQR1XS
M14 NIH6OoRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoPUTWM2OD1yLkS3NVch|ryP NYjHc5JpW0GQR1XS
SW962 NGi0T2tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWTtbHNoUUN3ME2wMlQ4OTlizszN M{jHe3NCVkeHUh?=
VM-CUB-1 MnLpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2DBXGlEPTB;MD60O|I5PiEQvF2= MnrQV2FPT0WU
A101D MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1iyd2lEPTB;MD60PFk3QCEQvF2= Mki3V2FPT0WU
DB MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVHubIZJUUN3ME2wMlUxODJzIN88US=> MVzTRW5ITVJ?
KNS-62 MlrQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUHJR|UxRTBwNUC1Olgh|ryP MWHTRW5ITVJ?
NCI-H460 MnHpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2PCSGlEPTB;MD61NFU6PyEQvF2= M2DrdHNCVkeHUh?=
MDA-MB-361 M3H3O2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnfjTWM2OD1yLkWwPFch|ryP NF;ZO|lUSU6JRWK=
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CW-2 NV32eWxZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVLJR|UxRTJwME[4O|Uh|ryP M{HsOHNCVkeHUh?=
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COLO-679 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYjRR|FVUUN3ME2yMlYyOjN|IN88US=> NVTGZ5BnW0GQR1XS
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LB2518-MEL Mom3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXHxSYFQUUN3ME2yMlY2PDB6IN88US=> MoLKV2FPT0WU
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LB996-RCC M{LrXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVzJR|UxRTNwNE[1NFIh|ryP MVXTRW5ITVJ?
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UACC-62 MlvNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXzJR|UxRTRwNE[1OFUh|ryP NH\C[IlUSU6JRWK=
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MEG-01 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlvWTWM2OD13LkC4N|I5KM7:TR?= MnLoV2FPT0WU
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ONS-76 NVXhNHhPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFjBXWlKSzVyPUWuOVU5ODFizszN NX:2bm1jW0GQR1XS
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COLO-741 NYjEOlU2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2DkbWlEPTB;NT63NVk6PSEQvF2= NVzUbHBIW0GQR1XS
HT-29 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkCxTWM2OD13LkiwOFc2KM7:TR?= MkPDV2FPT0WU
HCC1187 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MY\JR|UxRTVwOEGwNFgh|ryP NXHtXYJVW0GQR1XS
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NCI-H630 NETxZ3ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoDkTWM2OD14LkGxOFQyKM7:TR?= MnexV2FPT0WU
COLO-678 NH3O[ohIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1rwVmlEPTB;Nj6xOVA6OiEQvF2= M3XGSXNCVkeHUh?=
D-502MG NVfLTXh5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3XpeGlEPTB;Nj6xO|M{PiEQvF2= MUPTRW5ITVJ?
NB17 NGXvT4hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXPJR|UxRTZwMkS5OFMh|ryP MY\TRW5ITVJ?
SK-MEL-24 MmP1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlzXTWM2OD14LkK2OFkh|ryP MYnTRW5ITVJ?
NCI-SNU-5 NULJRWVzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MofZTWM2OD14LkK3NVE5KM7:TR?= M2ixfHNCVkeHUh?=
D-392MG M4LsU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUXJR|UxRTZwM{OzPVkh|ryP MUPTRW5ITVJ?
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OCI-AML2 NHnZTGJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmmxTWM2OD14LkS1OFQ2KM7:TR?= M1:4fnNCVkeHUh?=
HCC2157 NVL4RoVrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXqwTHk6UUN3ME22MlQ2PjF4IN88US=> NWP0dm81W0GQR1XS
C3A NGTwNW5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2f2UWlEPTB;Nj61NlAxPCEQvF2= MVHTRW5ITVJ?
NCI-H1650 MlL3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFnjSnJKSzVyPU[uO|A2OjJizszN MWfTRW5ITVJ?
DU-145 NG\zSZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{[wTWlEPTB;Nj63O|A{KM7:TR?= NWjEd4c3W0GQR1XS
COLO-829 NFP1XFhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4jCW2lEPTB;Nj63O|g4OyEQvF2= MkixV2FPT0WU
LS-1034 M4P1Zmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVjJR|UxRTdwMES0NVIh|ryP MVHTRW5ITVJ?
HCC1954 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUDJR|UxRTdwNEixN|Qh|ryP NHPDXZVUSU6JRWK=
KURAMOCHI NV\yW4pjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV7rWWJGUUN3ME23MlQ5PTN|IN88US=> M3[1ZXNCVkeHUh?=
Mo-T M{X4TWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkLJTWM2OD15LkWxOFk5KM7:TR?= MmnaV2FPT0WU
OE19 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Moe4TWM2OD15Lk[1OVA{KM7:TR?= MUXTRW5ITVJ?
COR-L105 MlXhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWPsbmxmUUN3ME23MlcyPDJzIN88US=> M173bHNCVkeHUh?=
NCI-H2342 NYPzN4FST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXTWdnV5UUN3ME23MlcyPTJzIN88US=> NXHCXJA2W0GQR1XS
U-87-MG NIKwWZBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYXHNZpDUUN3ME24MlA{OzN2IN88US=> M2rBNXNCVkeHUh?=
MKN28 M3;JNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NE\oTllKSzVyPUiuNVE2OTVizszN NG\ZbmNUSU6JRWK=
HT-1197 NFH3VXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn3NTWM2OD16LkK1PVA4KM7:TR?= NVXmSlVsW0GQR1XS
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NCI-H2029 NXn3XpJ[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXvJR|UxRTlwME[3OVkh|ryP MUjTRW5ITVJ?
TE-11 NVO3bVdMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUDJR|UxRTlwNEW1PFgh|ryP NITEPJRUSU6JRWK=
ZR-75-30 M1PWNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWC5OFR1UUN3ME25MlkzPjN2IN88US=> NFjhVI9USU6JRWK=
SW837 NFHEOVVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWTwUoFZUUN3ME2xNE4zOjl|IN88US=> MnvHV2FPT0WU
NCI-H1651 MnXXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIfTXZFKSzVyPUGwMlI{OjZizszN NXT5U2VZW0GQR1XS
NCI-H727 NFLJboFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGTWXWpKSzVyPUGwMlQ1QTVizszN MYTTRW5ITVJ?
NCI-H358 NWDpbpVVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoPpTWM2OD1zMD64N|Y2KM7:TR?= MV;TRW5ITVJ?
NCI-H2087 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVLMcWRRUUN3ME2xNU4xPDN2IN88US=> NG\kVFZUSU6JRWK=
FTC-133 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkXuTWM2OD1zMT60NFUyKM7:TR?= M2LufnNCVkeHUh?=
LAN-6 NXPjTG1oT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWXJR|UxRTF{LkGyOEDPxE1? NUX5fVNYW0GQR1XS
NCI-H1838 NX\FVJNMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIPoXJZKSzVyPUGyMlUyPTFizszN NWjoe|h[W0GQR1XS
Calu-6 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1r6c2lEPTB;MUSuNVAzOSEQvF2= M2XDSHNCVkeHUh?=
HCC70 M1zWRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M37WS2lEPTB;MUSuOFUyKM7:TR?= MUjTRW5ITVJ?
KARPAS-299 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWLFWnI1UUN3ME2xOU4zOTV7IN88US=> MUDTRW5ITVJ?
CAL-27 NV20c2loT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnnsTWM2OD1zNT6zPFQ{KM7:TR?= NHvLOmdUSU6JRWK=
NCI-H82 NF7sXoZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NITmcYpKSzVyPUG1MlU{OiEQvF2= MUXTRW5ITVJ?
no-10 NFHIXXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{jq[mlEPTB;MUWuPVM5KM7:TR?= Mn;XV2FPT0WU
U251 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWjWfFNyUUN3ME2xOk4{Pjd3IN88US=> NEHqUYJUSU6JRWK=
TE-9 NXHGXodwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFX3b5VKSzVyPUG3MlUyPjZizszN NVTC[pdqW0GQR1XS
TE-10 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1zv[GlEPTB;MUmuNFYxPyEQvF2= MV;TRW5ITVJ?
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ABC-1 NVjlSYh{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXvleZVKUUN3ME2yOk44QTV5IN88US=> NHHWfFlUSU6JRWK=
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SW1463 NIjtNFlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkHuTWM2OD1|Mz65PFMh|ryP M2i4eXNCVkeHUh?=
SW1417 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NETqbnZKSzVyPUO3MlA6PTJizszN NEL3VldUSU6JRWK=
NB12 NFXHfIpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHr2bJhKSzVyPUO5MlQ3PTVizszN M13MVHNCVkeHUh?=
NY NY\F[XVtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXLJR|UxRTRzLkOyNFEh|ryP MXXTRW5ITVJ?
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EGI-1 NInHTItIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWe3NkBp NXy5ZYU1TE2VTx?= NYC5RoRZUUN3ME21N{BvVQ>? NF;SfVEzPTF3MkK0OC=>
OCI/AML3 NXq4UFRVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlLYNE4zKM7:TR?= MV60PEBp M4\5V4VvcGGwY3XzJINmdGxiZ4Lve5RpKGmwaHnibZRqd25iaX7keYNm\CCkeTDz[Yx2dWW2aX7pZi=> NFfqO2UzPDd|OUO5Ny=>
U937 NXy4T5VKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXfVdYI2OC5{IN88US=> NELFUpk1QCCq NHP3OnVmdmijbnPld{Bk\WyuIHfyc5d1cCCrbnjpZol1cW:wIHnu[JVk\WRiYomgd4VtfW2ndHnubYI> NFXxUFUzPDd|OUO5Ny=>
MOLM13 NF;Fb4xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX:wMlIh|ryP MWq0PEBp NIfldHVmdmijbnPld{Bk\WyuIHfyc5d1cCCrbnjpZol1cW:wIHnu[JVk\WRiYomgd4VtfW2ndHnubYI> NHPDVZQzPDd|OUO5Ny=>
MCF7 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF3XSFQzOCCwTR?= NXr0fVFEPzJiaB?= M4[3bmZDWw>? MVny[YR2[2W|IITo[UBRTUySMT3t[YRq[XSnZDDpcoNz\WG|ZTDpckBk\WyuII\pZYJqdGm2eR?= NWHDOmNOOjR4OEiwOFY>
TamR NWnDXZo6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYjJR|UxRTF64pEJcm0> M4O5[|I1PDV5ME[5
MCF7-X  M3jZUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn\JTWM2OD1{NDDuUS=> MnGyNlQ1PTdyNkm=
T47D-tamR  M{mxbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYS2SWVvUUN3ME2xPUBvVQ>? NH7pOpIzPDR3N{C2PS=>
293T  M37ndGZ2dmO2aX;uJGF{e2G7 MoDDNlAhdk1? NFjjU4Y{KGh? MoWx[Il{enWydIOgRYt1NW2nZHnheIVlKFSLRj3JRUB{fGGkaXzpeJktKHS{YX7zcI9k[XSrb36sJIFv\CCjY4Tpeol1gQ>? NFL6cFUzPDN4M{S0PS=>
SW620 M1PwWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlHhNE4xODFvMUFOwG0> NVm5V4ZUOjRiaB?= NVfscZhvcW6qaXLpeJMhfGinIHPlcIwheHKxbHnm[ZJifGmxbjDhd{B1cGViY3;uZ4VvfHKjdHnvckBzcXOncx?= M{WxUVI1OzZ|NES5
Huh7  Mn34S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWS1MVExOCCwTR?= NYTDPXdqOC1{NDDo MorUSG1UVw>? MkfMbY5kemWjc3XzJINmdGxiZHXheIghcW5iYTDjc45k\W62cnH0bY9vNSCjbnSgeIlu\S2mZYDlcoRmdnRibXHucoVz NIGwXZozPDJ7N{OwNC=>
Hep3B  NFTs[mRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYXjcJJjPS1zMECgcm0> MlfJNE0zPCCq M4LPPWROW09? NFq0PGhqdmO{ZXHz[ZMh[2WubDDk[YF1cCCrbjDhJINwdmOnboTyZZRqd25vIHHu[EB1cW2nLXTldIVv\GWwdDDtZY5v\XJ? M4fhUVI1Ojl5M{Cw
Huh7  MXvGeY5kfGmxbjDBd5NigQ>? NHHwZ3kyODBibl2= NHHWNIQyOiCq MV;EUXNQ NIfzOW9qdmS3Y3XzJIF2fG:yaHHnfeKh NFjUSoYzPDJ7N{OwNC=>
U87-MG  NWLM[IVmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFLLU3c4OiCq MYDHTVUx6oDLPfMAjVc6KG6PwrC= NXq5XWlKOjR{NEm2OFA>
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RMS13 MXTBdI9xfG:|aYOgRZN{[Xl? Mlv0NUDPxE1? MkjFOFghcA>? MlSybY5kemWjc3XzJINmdGxiYYDvdJRwe2m|IHnu[JVk\WRiYomgRWJVNTd|Nx?= Mle3NlQyOzN{MUi=
RH30 M1rnbmFxd3C2b4Ppd{BCe3OjeR?= MVuxJO69VQ>? Moi2OFghcA>? Ml;wbY5kemWjc3XzJINmdGxiYYDvdJRwe2m|IHnu[JVk\WRiYomgRWJVNTd|Nx?= NEi0ZlEzPDF|M{KxPC=>
Hep-2 MnzmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3m0fVAvODFvMTFOwG0> MoOwNVIuQTZiaB?= MYPpcoNz\WG|ZYOgZ4VtdCCmZXH0bEBqdiCjIHPvcoNmdnS{YYTpc44uKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI> M2LkeFI1OTB|N{S5
SCC-9 NX6yfINbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn;vNE4xOS1zIN88US=> NF62fpoyOi17NjDo NVPybYxjcW6lcnXhd4V{KGOnbHyg[IVifGhiaX6gZUBkd26lZX70doF1cW:wLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{ NXLjRoozOjRzMEO3OFk>
Hep-2 NIfVbmVHfW6ldHnvckBCe3OjeR?= NHnCbZMxNjFxMD61JO69VQ>? M2TLdFI1KGh? MVLicI9kc3NibWTPVmMyKGGwZDDtWG9TSzJiYXP0bZZifGmxbh?= NWexelhPOjRzMEO3OFk>
SCC-9 MUTGeY5kfGmxbjDBd5NigQ>? NWP6dpdyOC5zL{CuOUDPxE1? MYSyOEBp NYjxZm1D[myxY3vzJI1VV1KFMTDhcoQhdVSRUlOyJIFkfGm4YYTpc44> MlTwNlQyODN5NEm=
PANC-1 M32ycGFxd3C2b4Ppd{BCe3OjeR?= NUe0eWZ3PTByIH7N NUSzWmVKPDhiaB?= NIfTU25{\W6|aYTpfoV{KHCjbnPy[YF1cWNiY3HuZ4VzKGOnbHzzJJRwKHKjZHnveIhmemGyeR?= M2P5eFI{QDh4Mkm0
RMG2 MlvNRZBweHSxc3nzJGF{e2G7 M{XsdlEuOTByMDDuUS=> NXHKPZpJPzJiaB?= NGjvN5VHSlN? M1;VZolvcGmkaYTzJJRp\SClZXzsJJZq[WKrbHn0fUBkd26lZX70doF1cW:wIHTldIVv\GWwdHz5 MXqyN|YyPTZ|MR?=
HAC2 M1y1NWFxd3C2b4Ppd{BCe3OjeR?= MXyxMVExODBibl2= NX:xSo83PzJiaB?= NIXmb4VHSlN? MkX5bY5pcWKrdIOgeIhmKGOnbHygeoli[mmuaYT5JINwdmOnboTyZZRqd25iZHXw[Y5l\W62bIm= M2\3U|I{PjF3NkOx
HT-29 M1vIb2Fxd3C2b4Ppd{BCe3OjeR?= M2LubVEh|ryP NFv1SFA4OiCq MU\heJRmdnWjdHXzJFUuTlVvaX7keYNm\CClZXzsJIRm[XSq MXSyNVk1QTF{MR?=
DLD1 M3TTWGFxd3C2b4Ppd{BCe3OjeR?= MnT1NUDPxE1? M3Hk[FczKGh? MXLheJRmdnWjdHXzJFUuTlVvaX7keYNm\CClZXzsJIRm[XSq MnTaNlE6PDlzMkG=
HT-29 NGnwSWdHfW6ldHnvckBCe3OjeR?= MnzLNUDPxE1? M3\wR|czKGh? NXG1SI5xcW6mdXPld{BifXSxcHjh[5khfG9iY3;u[oVzKGO7dH;wdo91\WO2aX;u NUjHO|NKOjF7NEmxNlE>
DLD1 MVzGeY5kfGmxbjDBd5NigQ>? NYKzfotZOSEQvF2= M4X2VVczKGh? MoLUbY5lfWOnczDheZRweGijZ4mgeI8h[2:wZnXyJIN6fG:ycn;0[YN1cW:w MWKyNVk1QTF{MR?=
Rh41 NIHST4hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1rl[WlEPTB;M{CuO{BvVQ>? MX6yNVM{PzZ5OR?=
Rh18 M2C4UGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mo\tTWM2OD12MT60JI5O M{DnT|IyOzN5Nke5
Rh30 M{O4Z2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3;XdWlEPTB;MUSgcm0> MXqyNVM{PzZ5OR?=
BT-12 NETBfVFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXTqe5hmUUN3ME2yOE44KG6P MmXLNlE{Ozd4N{m=
CHLA-266 NFHMRlhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF70[VdKSzVyPUGuNUBvVQ>? NH3TVGwzOTN|N{[3PS=>
TC-71 NXfKcnNET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{m5VGlEPTB;NE[uOUBvVQ>? MV6yNVM{PzZ5OR?=
CHLA-9 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH\JWYZKSzVyPUG1MlMhdk1? M1i4fFIyOzN5Nke5
CHLA-10 Mnj1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmHsTWM2OD1|Lkmgcm0> NIDLSlUzOTN|N{[3PS=>
CHLA-258 NXfPdXFVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIHSeZRKSzVyPUGwMlQhdk1? NHqyNYozOTN|N{[3PS=>
GBM2 NFfBPVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYLJR|UxRTRyLkegcm0> NWXYTmNROjF|M{e2O|k>
NB-1643 NXfJVY8yT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mk\DTWM2OD1|Mz64JI5O NV;ubmN[OjF|M{e2O|k>
NB-EBc1 NFvn[2ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH35OXVKSzVyPUK3MlEhdk1? M17nWlIyOzN5Nke5
CHLA-90 NH3kbJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVPQbIVYUUN3ME2zOE42KG6P M2X1bVIyOzN5Nke5
CHLA-136 NV;lZVdnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH\PUINKSzVyPUG2Nk43KG6P M3fidVIyOzN5Nke5
NALM-6 M2\pOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUTJR|UxRTN{Lkmgcm0> MVmyNVM{PzZ5OR?=
COG-LL-317 M4H3OWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX\JR|UxRTFwMzDuUS=> NF7wVVIzOTN|N{[3PS=>
RS4;11 M1rKPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3Pn[mlEPTB;M{Wgcm0> NXe3boI{OjF|M{e2O|k>
MOLT-4 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFjaNYlKSzVyPUiuOkBvVQ>? M2ezbVIyOzN5Nke5
CCRF-CEM M1O5Wmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGq3T5hKSzVyPUSuNUBvVQ>? NVey[257OjF|M{e2O|k>
Kasumi-1 M2HFPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWTJR|UxRTFwNDDuUS=> NGLzeHkzOTN|N{[3PS=>
Karpas-299 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEHNcoRKSzVyPUKxMlchdk1? MYKyNVM{PzZ5OR?=
Ramos-RA1 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{G4[mlEPTB;OD6yJI5O NIrkV20zOTN|N{[3PS=>
H838 M4nQcGZ2dmO2aX;uJGF{e2G7 MkfENUDPxE1? NXHYVJF6PDhiaB?= MnPwbY5lfWOnczDj[YxtKGG3dH;wbIFogQ>? NUjYfZBpOjB|NkSxNVM>
H838 NF;xbI1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXq4PZBoOC1|IN88US=> M4L2dFczKGh? NHrKO4ZqdmS3Y3XzJIdzd3e2aDDpcohq[mm2aX;uJIFv\CCjdYTvdIhi\3l? NUW0enRLOjByMki4OVQ>
A549  M2Lxc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn[zNE0{KM7:TR?= MlfBO|IhcA>? NX\lXZQ1cW6mdXPld{Boem:5dHigbY5pcWKrdHnvckBidmRiYYX0c5Bp[We7 NGXyV5IzODB{OEi1OC=>

... Click to View More Cell Line Experimental Data

In vivo AZD8055 inhibits the pS6 and pAkt in U87MG and A549 xenografts at 2.5/10 mg/kg, which leads to tumor growth inhibition. AZD8055 shows significant antitumor activity in many xenografts, including U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA at a dose of 10/20 mg/kg. [1] AZD8055 induces ~40% reduction in tumour volume, accompanied by ablation of phosphorylation of Akt, S6K and SGK protein kinases. Administration of AZD8055 (5mg/kg, Bid) and SAHA (100 mg/kg/d) results in complete tumor growth inhibition in PTEN+/−LKB1+/hypo xenografts without side effects on mice by inhibition of mTORC1 and mTORC2 signaling. [4]

Protocol

Kinase Assay:[1]
+ Expand

Cell-based determinations of mTOR inhibition:

A high-throughput screening cell-based assay is developed using MDA-MB-468 cells to detect mTORC1 and mTORC2 activity. Cells are exposed for 2 hours to increasing concentrations of AZD8055. At the end of the incubation period, cells are fixed, washed, and probed with antibodies against S473 pAkt or against S235/236 phosphorylated S6 (pS6). Levels of phosphorylation are assessed using an Acumen laser scanning cytometer.
Cell Research:[1]
+ Expand
  • Cell lines: U87MG, A549 and H838 cells
  • Concentrations: 1 nM - 1 μM
  • Incubation Time: 72 to 96 hours
  • Method: Cells are exposed to AZD8055 for 72 to 96 hours and stained for cell nuclei (0.03 mg/mL Hoechst 33342) and acidic vesicles (1 μg/mL acridine orange). Images are captured at 450 and 536 nm on an ArrayScan II platform, and the percentage of acidic vesicles and the number of cells are quantified. For LC3 assessment, cells are exposed to e64d/pepstatin (10 μg/mL) for 30 to 90 min before incubation with AZD8055. Cells are lysed on ice and analyzed by immunoblotting.
    (Only for Reference)
Animal Research:[1]
+ Expand
  • Animal Models: U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA U87-MG and A549 are established in pathogen-free, female nude mice (nu/nu:Alpk).
  • Formulation: Dissolved in captisol (CyDex, pH 3.0) and diluted to a final captisol concentration of 30% (w/v)
  • Dosages: 2.5-20 mg/kg
  • Administration: Oral gavage once or twice daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 50 mg/mL warmed (107.4 mM)
Ethanol 3 mg/mL (6.44 mM)
Water slightly soluble or insoluble
In vivo Add solvents individually and in order:
4% DMSO+30% PEG 300+ddH2O
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 465.54
Formula

C25H31N5O4

CAS No. 1009298-09-2
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01316809 Completed Glioblastoma Multiforme|Anaplastic Astrocytoma|Anaplastic Oligodendroglioma|Malignant Glioma|Brainstem Glioma National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) March 4, 2011 Phase 1
NCT00999882 Completed Cancer|Advanced Hepatocellular Carcinoma AstraZeneca October 2009 Phase 1
NCT00973076 Completed Cancer|Solid Tumors|Advanced Solid Malignancies AstraZeneca August 2009 Phase 1
NCT00731263 Completed Solid Tumors AstraZeneca July 2008 Phase 1
NCT01194193 Withdrawn Cancer|Advanced Solid Tumours|Lymphomas AstraZeneca null Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID