AZD8055

Catalog No.S1555

AZD8055 Chemical Structure

Molecular Weight(MW): 465.54

AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. Phase 1.

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In DMSO USD 156 In stock
USD 120 In stock
USD 370 In stock
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Cited by 38 Publications

8 Customer Reviews

  • PC3-LN4 cells were treated with GSK690693 (5 uM) alone, or in combination with PP242 (2 uM) or AZD8055 (1 uM) for 24 h, and immunoblotting performed.

    Cancer Res 2013 73(11), 3402-11. AZD8055 purchased from Selleck.

    HUVEC were treated with either RAD001 or AZD8055 before PA treatment. p53, GPX1, p53 acetylation, and subcellular PTEN were analyzed with corresponding antibodies. The blot is a representative of the results of three experiments. Values (mean盨D) are expressed as fold over untreated control. *p<0.05 versus their corresponding control groups in the one-way ANOVA.

    Antioxid Redox Signal 2014 20(9), 1382-95. AZD8055 purchased from Selleck.

  • (A) HCT116 or MDA-MB-468 cells were treated with 500 nM AZD8055 for the indicated times. Cell lysates were immunoblotted with the indicated antibodies. (B) HCT116 or MDA-MB-468 cells were treated with 50 nM rapamycin. 500 nM AZD8055, 1 uM MK2206 or dimethyl sulfoxide (DMSO) as control for 12 h. Cell lysates were immunoblotted with the indicated antibodies.

    Oncotarget 2014 5(15), 6015-27. AZD8055 purchased from Selleck.

    CSCs+24/44/ESA at 5,000 cells per well with stem cell culture media in triplicate in an ultra-low adhesion plate were treated with 5 umol/L BMS-777607, 1 umol/L AZD8055 alone, or in their different combinations. Cells were cultured for 72 hours. Cell viability was determined by the MTS assay. Percentages of polyploid cells were determined by counting 300 cells from two different regions. Results shown here were from one of two experiments with similar results.

    Mol Cancer Ther 2014 13(1), 37-48. AZD8055 purchased from Selleck.

  • mTOR kinase inhibitor AZD8055 activates PI3K accompanied with induction of expression of EGFR, HER2, HER3 and IRS1. Serum-deprived CHO-EGFP-AKT cells were incubated with 50 nM AZD8055 for 24 hr. The EGFP signal was detected using confocal microscopy. The white arrows indicate EGFP-AKT located on cellular membrane.

    Int J Cancer 2014 135(10), 2462-74. AZD8055 purchased from Selleck.

    mTOR, P70S6K1, 4E-BP1 and PCNA activation in the basilar artery of SAH. Top, Western blotting bands of antibodies, bottom, density of the protein band from each group. A, mTOR activation in the basilar artery demonstrated an elevated expression of mTOR on day 7 following SAH in SAH and SAH+DMSO animals. The mTOR inhibitors, Rapamycin and AZD8055 reduced the enhanced expression of mTOR (pb0.05, ANOVA). B. P70S6K1 activation. C. 4E-BP1 activation. D. PCNA activation. Rapamycin and AZD8055 significantly decreased the expression of P70S6K1, 4E-BP1 and PCNA compared with the SAH and SAH+DMSO (pb0.05, respectively; ANOVA). There was no significant difference between the two inhibitors in mTOR, P70S6K1, 4E-BP1 and PCNA expression.

    Exp Neurol 2012 233, 799-806. AZD8055 purchased from Selleck.

  • Induction of TCTP expression through 5-FU or oxaliplatin is inhibited by mTOR kinase inhibitors. HCT116 cells were treated for 48 h with 5-FU or oxaliplatin at the indicated concentrations. Where indicated, AZD 8055 (100 nM) or PP242 (1 μM) were added. TCTP levels were assessed by western blotting; signals were quantified and normalised against α-tubulin as a loading control. As a readout of mTOR activity, phosphorylation of the ribosomal protein S6 at Ser 240/244 was assessed using a phospho-specific antibody. a. Representative western blots demonstrating the alteration of the levels of TCTP protein and of phospho-ribosomal protein S6, respectively, in relation to the drug concentration.

    Cell Commun Signal, 2017.. AZD8055 purchased from Selleck.

    After starved in serum-free medium for 24h , A549 cells incubated with the indicated concentrations of AZD8055 for 3h , followed by 20-minute stimolation of 100ng/ml EGF

     
     

     

    Dr. Zhang of Tianjin Medical University. AZD8055 purchased from Selleck.

Purity & Quality Control

Choose Selective mTOR Inhibitors

Biological Activity

Description AZD8055 is a novel ATP-competitive mTOR inhibitor with IC50 of 0.8 nM in MDA-MB-468 cells with excellent selectivity (∼1,000-fold) against PI3K isoforms and ATM/DNA-PK. Phase 1.
Features First drug to inhibit both types of mTOR protein.
Targets
mTOR (truncated) [1]
(MDA-MB-468 cells)
mTOR (full length) [1]
(MDA-MB-468 cells)
0.13 nM 0.8 nM
In vitro

AZD8055 shows low activity (∼1,000-fold) against all PI3K isoforms (α, β, γ, δ) and other members of the PI3K-like kinase family (ATM and DNA-PK). AZD8055 inhibits the phosphorylation of mTORC1 (p70S6K and 4E-BP1) as well as phosphorylation of the mTORC2 (Akt) and downstream proteins. The rapamycin-resistant T37/46 phosphorylation sites on 4E-BP1 can be fully inhibited by AZD8055, resulting in significant inhibition of cap-dependent translation. AZD8055 potently inhibits proliferation in U87MG, A549 and H838 cells with IC50 of 53, 50 and 20 nM, respectively. AZD8055 also induces autophagy and increased LC3-II levels in H838 and A549 cells. [1] AZD8055 decreases AML blast cell proliferation and cell cycle progression, reduces the clonogenic growth of leukemic progenitors and induces caspase-dependent apoptosis in leukemic cells but not in normal immature CD34+ cells. [2] AZD8055 indicates inhibitory against the pediatric preclinical testing program (PPTP) cell lines with IC50 of 24.7 nM and induces significant differences in EFS distribution. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LU-139 MmLES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHvUVmhKSzVyPUCuNFI{OzFizszN M3LrdHNCVkeHUh?=
NCI-H720 NYLBdZlyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGW3XYdKSzVyPUCuNFI6OzhizszN Ml3GV2FPT0WU
KARPAS-45 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHW1WpJKSzVyPUCuNFM{PzFizszN M{jFcHNCVkeHUh?=
EW-3 NVvadHJXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1zUUmlEPTB;MD6wOFA2PiEQvF2= M1\q[HNCVkeHUh?=
SBC-1 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVPJR|UxRTBwMES1PFEh|ryP NYTXRpFGW0GQR1XS
NCI-H209 NGTrRYdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnPPTWM2OD1yLkC1OFU{KM7:TR?= MWjTRW5ITVJ?
EoL-1-cell M{DQZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXf6SZBHUUN3ME2wMlA3PTZ7IN88US=> MkXaV2FPT0WU
H9 MoXSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGP3cXhKSzVyPUCuNFY3QTJizszN NYfSR5dbW0GQR1XS
AN3-CA NFryfHhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWfJR|UxRTBwMEe5OVEh|ryP M1rkbnNCVkeHUh?=
HH NIjERWVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYPJR|UxRTBwMEiwNFkh|ryP NFTid3ZUSU6JRWK=
KU812 M4Lp[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHXBc5BKSzVyPUCuNFgxPDNizszN NF:1bIhUSU6JRWK=
DU-4475 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYD1bI52UUN3ME2wMlA5ODR5IN88US=> NH7yOmRUSU6JRWK=
CTV-1 NXjwOnRJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH\xN21KSzVyPUCuNFg3PDFizszN M3jJVHNCVkeHUh?=
BV-173 NX2ycoFYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUPJR|UxRTBwMEmwOlEh|ryP NUfU[XpHW0GQR1XS
ES5 NYG0UHJVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWnmPYdVUUN3ME2wMlA6OzVizszN MoDhV2FPT0WU
G-402 NHnKXG1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYnJR|UxRTBwMEm5PEDPxE1? NWfwbXY2W0GQR1XS
MV-4-11 NXrUSYptT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{DJ[WlEPTB;MD6xNFM1PyEQvF2= NULKVFlXW0GQR1XS
NB69 M4L3VGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3XZN2lEPTB;MD6xN|Uh|ryP NGDiOm1USU6JRWK=
EGI-1 NWe1T2hOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFm2XmhKSzVyPUCuNVM2OzFizszN M{\kT3NCVkeHUh?=
EW-18 Mn7mS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUGySmpWUUN3ME2wMlE1ODd|IN88US=> M{HubXNCVkeHUh?=
ME-180 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWixZVRNUUN3ME2wMlE1PjhzIN88US=> NVvRT5lLW0GQR1XS
D-283MED MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2KwcWlEPTB;MD6xOFczOyEQvF2= NXviNFZkW0GQR1XS
NB14 NVG5VmFDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmTiTWM2OD1yLkG2OFQ4KM7:TR?= M33jS3NCVkeHUh?=
Ramos-2G6-4C10 M{nNSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVHHVWZiUUN3ME2wMlE3QTh|IN88US=> MXPTRW5ITVJ?
NBsusSR NVzEWJgyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M375OGlEPTB;MD6xO|AyQSEQvF2= NGD6S4NUSU6JRWK=
LAMA-84 M1i4Vmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYnJR|UxRTBwMUiwN|Ih|ryP Mmj6V2FPT0WU
NCI-H1581 MmLNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4K5XGlEPTB;MD6xPFk3PiEQvF2= MnLkV2FPT0WU
A204 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{n6dWlEPTB;MD6xPFk4KM7:TR?= MkHvV2FPT0WU
A549 NIPDNXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGe0UoZKSzVyPUCuNVk2PSEQvF2= NXvuS5BOW0GQR1XS
CAL-51 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYnJR|UxRTBwMUm2OVEh|ryP MVHTRW5ITVJ?
NKM-1 Ml;0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHrFdXpKSzVyPUCuNlAxOjlizszN NHS4WGpUSU6JRWK=
LoVo NXrqTGRmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3m2dGlEPTB;MD6yNFIxOiEQvF2= NVHp[lJ[W0GQR1XS
HAL-01 NHrFO4pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGjYZZFKSzVyPUCuNlAzQTdizszN M3HpXnNCVkeHUh?=
HCC2218 M4Tjbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIe1co9KSzVyPUCuNlA3QDhizszN M1vQO3NCVkeHUh?=
BEN NYfmS5I4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFTUbZhKSzVyPUCuNlEzQTNizszN NXrNN4xlW0GQR1XS
LC-2-ad M1XNPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFfFeItKSzVyPUCuNlIxQDRizszN MYPTRW5ITVJ?
LXF-289 MnK1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYTrXmxLUUN3ME2wMlI2ODRzIN88US=> MnX4V2FPT0WU
HGC-27 Ml:5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXnJR|UxRTBwMk[0NVQh|ryP NHzGWFRUSU6JRWK=
LK-2 NXzoTFFOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWfidYdNUUN3ME2wMlI3QDJ2IN88US=> NITtU|hUSU6JRWK=
CTB-1 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV\iXXpZUUN3ME2wMlI4OjF3IN88US=> MYPTRW5ITVJ?
697 MlTPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWDJR|UxRTBwMkezOlch|ryP M1TSVnNCVkeHUh?=
ES7 NU\PXZp[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWW3S4x7UUN3ME2wMlI4Ozh|IN88US=> MlrjV2FPT0WU
MG-63 M1TWPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXHpT3RzUUN3ME2wMlI4PDB5IN88US=> NIL3T5VUSU6JRWK=
T47D M1TNUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3;OZmlEPTB;MD6yO|Q4PSEQvF2= NULZNXlZW0GQR1XS
RPMI-8226 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYrJR|UxRTBwMki1PFkh|ryP M1;mR3NCVkeHUh?=
NCI-H292 NXG0[IlpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M17Yd2lEPTB;MD6yPVE3PCEQvF2= NXO1eXlkW0GQR1XS
HT NWnXcnBMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{HrWGlEPTB;MD6yPVM5KM7:TR?= NEnTNYpUSU6JRWK=
A3-KAW M2\WNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV20TGVLUUN3ME2wMlI6PzRizszN NH73SXFUSU6JRWK=
LB2241-RCC M33sSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVHxe5ZMUUN3ME2wMlMxOTl6IN88US=> MXHTRW5ITVJ?
BB65-RCC MoPJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUnnV4lDUUN3ME2wMlMxQDB3IN88US=> M3\BSXNCVkeHUh?=
G-401 NWDhV3l7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEW5UFhKSzVyPUCuN|A6QDZizszN NFzuVnVUSU6JRWK=
TYK-nu NVLG[oFuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXXoWHF7UUN3ME2wMlMyQDZ2IN88US=> MmXuV2FPT0WU
NCI-SNU-1 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NU\qPWI3UUN3ME2wMlMzOzNzIN88US=> MnfpV2FPT0WU
CHL-1 NH3MUplIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mo\rTWM2OD1yLkOyO|A{KM7:TR?= M4LoVXNCVkeHUh?=
P30-OHK Mk\YS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1HMbmlEPTB;MD6zNlcxPSEQvF2= M163bnNCVkeHUh?=
PA-1 NH2xRopIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1r2R2lEPTB;MD6zN|YyQSEQvF2= MkjUV2FPT0WU
LB1047-RCC NHjCenlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVHjXmF3UUN3ME2wMlM1ODR5IN88US=> M2iwbnNCVkeHUh?=
EM-2 NHrxSZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnrOTWM2OD1yLkO0NVg5KM7:TR?= MWrTRW5ITVJ?
VA-ES-BJ MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3naPWlEPTB;MD6zOFI3QCEQvF2= M2DSe3NCVkeHUh?=
NCI-H520 NG\oZ5hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXLiSVZKUUN3ME2wMlM1QDN2IN88US=> NITjRmJUSU6JRWK=
ECC10 NWG3N3VuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVvJR|UxRTBwM{WwN|Uh|ryP M{Xk[XNCVkeHUh?=
LNCaP-Clone-FGC NGXXcVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlrZTWM2OD1yLkO1N|E1KM7:TR?= M121b3NCVkeHUh?=
ES8 NH7xRmpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4DTcGlEPTB;MD6zOlU2OyEQvF2= MW\TRW5ITVJ?
HuO-3N1 M4XDUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVjJR|UxRTBwM{izO|kh|ryP M3\QXnNCVkeHUh?=
L-363 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlPzTWM2OD1yLkO5NFI3KM7:TR?= NXW4eFMzW0GQR1XS
SK-LU-1 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUDJR|UxRTBwM{myNFgh|ryP M2rHXnNCVkeHUh?=
MCF7 NVvsWmJxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUXz[nY3UUN3ME2wMlQxPTNizszN NIO5[GJUSU6JRWK=
HuO9 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWPJR|UxRTBwNEC2Olch|ryP NInuN4FUSU6JRWK=
BHT-101 Mmf1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3nTe2lEPTB;MD60NFc5PyEQvF2= MmjyV2FPT0WU
MZ2-MEL NHTGRlBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NI\qeY1KSzVyPUCuOFA5QDJizszN M3ixWHNCVkeHUh?=
NOS-1 MnnqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3fBSGlEPTB;MD60NVA4PCEQvF2= MmLVV2FPT0WU
NEC8 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFvhdpNKSzVyPUCuOFE3QTVizszN NGXGRnpUSU6JRWK=
COR-L88 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlTrTWM2OD1yLkSyNlY4KM7:TR?= NHrPVpVUSU6JRWK=
MFM-223 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFfpbXNKSzVyPUCuOFI3OTdizszN MWjTRW5ITVJ?
HSC-3 M2jpTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV3JR|UxRTBwNEK5NFgh|ryP NGjiXpZUSU6JRWK=
OVCAR-5 MnK4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mlz4TWM2OD1yLkSyPVM{KM7:TR?= MnHNV2FPT0WU
MFE-280 NVSyT4lOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVjJR|UxRTBwNEOwN|Ih|ryP M3X1RXNCVkeHUh?=
KP-N-YS NUHFSHA2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHnubZpKSzVyPUCuOFMxPyEQvF2= NGnvVnBUSU6JRWK=
EPLC-272H MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHS0Ro1KSzVyPUCuOFMxPzdizszN M33WNnNCVkeHUh?=
KE-37 NIDLe|RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEL5WolKSzVyPUCuOFMzPDdizszN M3fwXXNCVkeHUh?=
HuH-7 MnnTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXnuZVA5UUN3ME2wMlQ{Ozd{IN88US=> MX3TRW5ITVJ?
CHP-212 NHH5R5VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmrhTWM2OD1yLkSzOlk4KM7:TR?= NHzRU2RUSU6JRWK=
SK-MES-1 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4\tUmlEPTB;MD60N|g1QCEQvF2= MVXTRW5ITVJ?
SJSA-1 NYi4WHBtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHOwOIpKSzVyPUCuOFQ2PTRizszN MonnV2FPT0WU
QIMR-WIL M{TZ[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXrFVVg4UUN3ME2wMlQ2OTVizszN MYDTRW5ITVJ?
ES4 NGD5c2dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4KzbmlEPTB;MD60OVM{PiEQvF2= M3nxTHNCVkeHUh?=
TI-73 MnjaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3;0XGlEPTB;MD60OVY5PiEQvF2= NH:5UGRUSU6JRWK=
COLO-684 M3LKbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2L2SGlEPTB;MD60OVczPiEQvF2= MkHFV2FPT0WU
GCIY MljUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEHLU5ZKSzVyPUCuOFU4PTRizszN NUXUR45EW0GQR1XS
JVM-3 Mnr4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mm\FTWM2OD1yLkS2NlkzKM7:TR?= NHHMbY9USU6JRWK=
MPP-89 NUniUIEzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MknoTWM2OD1yLkS2PVQ4KM7:TR?= M4nLNXNCVkeHUh?=
M14 NGXke|lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXfJR|UxRTBwNEexO{DPxE1? NWnGWGtJW0GQR1XS
SW962 Mo\XS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHTQTlJKSzVyPUCuOFcyQSEQvF2= NHT6SHVUSU6JRWK=
VM-CUB-1 MnzTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGHTSI9KSzVyPUCuOFczQDZizszN M{LOVnNCVkeHUh?=
A101D NELjV3pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXPJR|UxRTBwNEi5Olgh|ryP MmPXV2FPT0WU
DB MlLBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX7DNZNZUUN3ME2wMlUxODJzIN88US=> M4rZSHNCVkeHUh?=
KNS-62 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnjoTWM2OD1yLkWwOVY5KM7:TR?= NFTGVplUSU6JRWK=
NCI-H460 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVeyToZbUUN3ME2wMlUxPTl5IN88US=> NGDZS4tUSU6JRWK=
MDA-MB-361 M3jXc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFnlRndKSzVyPUCuOVA5PyEQvF2= M2myd3NCVkeHUh?=
DOK MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVPJR|UxRTBwNUGzN|Mh|ryP MkTlV2FPT0WU
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T47D-tamR  MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml;yTWM2OD1zOTDuUS=> M1XINVI1PDV5ME[5
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DLD1 NFTmdXdHfW6ldHnvckBCe3OjeR?= NFTKU3oyKM7:TR?= NUf0SlBFPzJiaB?= M4\mUolv\HWlZYOgZZV1d3CqYXf5JJRwKGOxbn\ldkBkgXSxcILveIVkfGmxbh?= MVSyNVk1QTF{MR?=
Rh41 NHLUT3JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NILCXpJKSzVyPUOwMlchdk1? M2\QU|IyOzN5Nke5
Rh18 NEOwXZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEG1eHRKSzVyPUSxMlQhdk1? NIHkR40zOTN|N{[3PS=>
Rh30 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFnFO3NKSzVyPUG0JI5O M{D1TFIyOzN5Nke5
BT-12 M4H5b2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1KwUGlEPTB;MkSuO{BvVQ>? MWKyNVM{PzZ5OR?=
CHLA-266 NUXDUpBST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4jFT2lEPTB;MT6xJI5O NXfsflNGOjF|M{e2O|k>
TC-71 NX7MclN1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1XnZmlEPTB;NE[uOUBvVQ>? Mn3sNlE{Ozd4N{m=
CHLA-9 M{XiZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFe1N45KSzVyPUG1MlMhdk1? MkixNlE{Ozd4N{m=
CHLA-10 M3i5OWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlPNTWM2OD1|Lkmgcm0> MViyNVM{PzZ5OR?=
CHLA-258 NGi0WFdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmTUTWM2OD1zMD60JI5O MoHiNlE{Ozd4N{m=
GBM2 M4DSTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUnJR|UxRTRyLkegcm0> NV[wTGF5OjF|M{e2O|k>
NB-1643 M4\zOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYTJR|UxRTN|Lkigcm0> MlfoNlE{Ozd4N{m=
NB-EBc1 M1XPfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWHJR|UxRTJ5LkGgcm0> M4W0[|IyOzN5Nke5
CHLA-90 NHPtfnlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWfmNHd3UUN3ME2zOE42KG6P MXWyNVM{PzZ5OR?=
CHLA-136 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVnJR|UxRTF4Mj62JI5O Mkm0NlE{Ozd4N{m=
NALM-6 M2K2OGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWTvcmpxUUN3ME2zNk46KG6P MXKyNVM{PzZ5OR?=
COG-LL-317 M2H6cmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkO3TWM2OD1zLkOgcm0> M4\BW|IyOzN5Nke5
RS4;11 NUnmR2RsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4HvOmlEPTB;M{Wgcm0> NWXZeVUzOjF|M{e2O|k>
MOLT-4 MljqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVfJR|UxRThwNjDuUS=> NWrqZZJxOjF|M{e2O|k>
CCRF-CEM NVizc|ZmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{XSb2lEPTB;ND6xJI5O MWOyNVM{PzZ5OR?=
Kasumi-1 NYDSXoczT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF[1TY1KSzVyPUGuOEBvVQ>? MXWyNVM{PzZ5OR?=
Karpas-299 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MULJR|UxRTJzLkegcm0> MoGwNlE{Ozd4N{m=
Ramos-RA1 NGT1NG1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHPOZXJKSzVyPUiuNkBvVQ>? Mn:xNlE{Ozd4N{m=
H838 NEi4WYxHfW6ldHnvckBCe3OjeR?= NWrWe4xxOSEQvF2= MU[0PEBp NV33[G05cW6mdXPld{Bk\WyuIHH1eI9xcGGpeR?= M1\lflIxOzZ2MUGz
H838 Mm\US5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mke1NE0{KM7:TR?= M3;xU|czKGh? MnPRbY5lfWOnczDndo94fGhiaX7obYJqfGmxbjDhcoQh[XW2b4DoZYd6 NWe1Z5VGOjByMki4OVQ>
A549  M2DjRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX;Vb2VPOC1|IN88US=> NEL4bJk4OiCq M1zvNolv\HWlZYOg[5Jwf3SqIHnubIljcXSrb36gZY5lKGG3dH;wbIFogQ>? M3P6[FIxODJ6OEW0

... Click to View More Cell Line Experimental Data

In vivo AZD8055 inhibits the pS6 and pAkt in U87MG and A549 xenografts at 2.5/10 mg/kg, which leads to tumor growth inhibition. AZD8055 shows significant antitumor activity in many xenografts, including U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA at a dose of 10/20 mg/kg. [1] AZD8055 induces ~40% reduction in tumour volume, accompanied by ablation of phosphorylation of Akt, S6K and SGK protein kinases. Administration of AZD8055 (5mg/kg, Bid) and SAHA (100 mg/kg/d) results in complete tumor growth inhibition in PTEN+/−LKB1+/hypo xenografts without side effects on mice by inhibition of mTORC1 and mTORC2 signaling. [4]

Protocol

Kinase Assay:[1]
+ Expand

Cell-based determinations of mTOR inhibition:

A high-throughput screening cell-based assay is developed using MDA-MB-468 cells to detect mTORC1 and mTORC2 activity. Cells are exposed for 2 hours to increasing concentrations of AZD8055. At the end of the incubation period, cells are fixed, washed, and probed with antibodies against S473 pAkt or against S235/236 phosphorylated S6 (pS6). Levels of phosphorylation are assessed using an Acumen laser scanning cytometer.
Cell Research:[1]
+ Expand
  • Cell lines: U87MG, A549 and H838 cells
  • Concentrations: 1 nM - 1 μM
  • Incubation Time: 72 to 96 hours
  • Method: Cells are exposed to AZD8055 for 72 to 96 hours and stained for cell nuclei (0.03 mg/mL Hoechst 33342) and acidic vesicles (1 μg/mL acridine orange). Images are captured at 450 and 536 nm on an ArrayScan II platform, and the percentage of acidic vesicles and the number of cells are quantified. For LC3 assessment, cells are exposed to e64d/pepstatin (10 μg/mL) for 30 to 90 min before incubation with AZD8055. Cells are lysed on ice and analyzed by immunoblotting.
    (Only for Reference)
Animal Research:[1]
+ Expand
  • Animal Models: U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA U87-MG and A549 are established in pathogen-free, female nude mice (nu/nu:Alpk).
  • Formulation: Dissolved in captisol (CyDex, pH 3.0) and diluted to a final captisol concentration of 30% (w/v)
  • Dosages: 2.5-20 mg/kg
  • Administration: Oral gavage once or twice daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 50 mg/mL warmed (107.4 mM)
Ethanol 3 mg/mL (6.44 mM)
Water Insoluble
In vivo Add solvents individually and in order:
4% DMSO+30% PEG 300+ddH2O
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 465.54
Formula

C25H31N5O4

CAS No. 1009298-09-2
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01316809 Completed Glioblastoma Multiforme|Anaplastic Astrocytoma|Anaplastic Oligodendroglioma|Malignant Glioma|Brainstem Glioma National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) March 4, 2011 Phase 1
NCT00999882 Completed Cancer|Advanced Hepatocellular Carcinoma AstraZeneca October 2009 Phase 1
NCT00973076 Completed Cancer|Solid Tumors|Advanced Solid Malignancies AstraZeneca August 2009 Phase 1
NCT00731263 Completed Solid Tumors AstraZeneca July 2008 Phase 1
NCT01194193 Withdrawn Cancer|Advanced Solid Tumours|Lymphomas AstraZeneca null Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID