Nicotinamide (Vitamin B3)
Catalog No.S1899 Synonyms: Niacinamide, Vitamin PP, Nicotinic acid amide
Molecular Weight(MW): 122.12
Nicotinamide (Vitamin B3), a water-soluble vitamin, is an active component of coenzymes NAD and NADP, and also act as an inhibitor of sirtuins.
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Immunoprecipitation-western blot for analyzing acetylated Snail in Snail-transfected 293T cells (upper) and FaDu-Snail cells (lower) treated with a vehicle control (Ctrl), a HDAC inhibitor TSA 5 nM for 8 hr, a SIRT1 inhibitor NAM(Nicotinamide) 10 mM for 8 hr, or in combination.
Cancer Cell,2014, 26(4):534-48.. Nicotinamide (Vitamin B3) purchased from Selleck.
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Choose Selective Sirtuin Inhibitors
|Description||Nicotinamide (Vitamin B3), a water-soluble vitamin, is an active component of coenzymes NAD and NADP, and also act as an inhibitor of sirtuins.|
Nicotinamide strongly inhibits yeast silencing, increases rDNA recombination, and shortens replicative life span to that of a sir2 mutant. Nicotinamide abolishes silencing and leads to an eventual delocalization of Sir2 even in G(1)-arrested cells, demonstrating that silent heterochromatin requires continual Sir2 activity.  Nicotinamide results in a twofold increase in DNA content and a threefold increase in insulin content in the fetal cells. Nicotinamide induces differentiation and maturation of human fetal pancreatic islet cells.  Nicotinamide regulates sirtuins by switching between deacetylation and base exchange. Nicotinamide switching is quantitated for the Sir2s from Archeaglobus fulgidus (Sir2Af2), Saccharomyces cerevisiae (Sir2p), and mouse (Sir2alpha).  Nicotinamide selectively reduces a specific phospho-species of tau (Thr231) that is associated with microtubule depolymerization in Alzheimer's disease transgenic mice, in a manner similar to inhibition of SirT1. Nicotinamide also dramatically increases acetylated alpha-tubulin, a primary substrate of SirT2, and MAP2c in Alzheimer's disease transgenic mice, both of which are linked to increased microtubule stability.  Nicotinamide fosters DNA integrity and maintains phosphatidylserine membrane asymmetry to prevent cellular inflammation, cellular phagocytosis and vascular thrombosis. Nicotinamide both prevents and reverses neuronal and vascular cell injury. 
|In vivo||In the mouse, nicotinamide given i.p. at doses of 100-500 mg/kg showed biphasic elimination with dose-dependent changes in half-life. The initial half-life increased significantly (P <0.05) from 0.8 to 2 h and the terminal half-life increased from 3.4 to 5.6 h over the dose range studied. Clearance, however, decreased significantly from 0.3 to 0.24 L/kg/h only at the highest dose. Peak concentrations increased in a dose-dependent manner from 1,000 to 4,800 nmol/ml. The bioavailability given via the i.p. as compared with the i. v. route was close to 100%.|
-  Bitterman KJ, et al. J Biol Chem, 2002, 277(47), 45099-45107.
-  Otonkoski T, et al. J Clin Invest, 1993, 92(3), 1459-1466.
-  Sauve AA, et al. Biochemistry, 2003, 42(31), 9249-9256.
|In vitro||DMSO||24 mg/mL (196.52 mM)|
|Water||24 mg/mL (196.52 mM)|
|Ethanol||24 mg/mL (196.52 mM)|
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
|Synonyms||Niacinamide, Vitamin PP, Nicotinic acid amide|
Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).
Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )
* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).
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Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
Clinical Trial Information
|NCT Number||Recruitment||Conditions||Sponsor/Collaborators||Start Date||Phases|
|NCT03019666||Not yet recruiting||Multiple Myeloma|Non-Hodgkin Lymphoma|Diffuse Large B Cell Lymphoma|Mantle-Cell Lymphoma|Follicular Lymphoma|Indolent B Cell Lymphoma|Primary Mediastinal Lymphoma|Lymphoplasmacytic Lymphoma||Masonic Cancer Center, University of Minnesota||March 2017||Phase 1|
|NCT02835664||Recruiting||Obesity|Insulin Resistance||Maastricht University Medical Center|Dutch Heart Foundation||December 2016||--|
|NCT02942888||Not yet recruiting||Mild Cognitive Impairment|NAD||The University of Texas Health Science Center at San Antonio|University of Texas|South Texas Veterans Health Care System||October 2016||--|
|NCT02721537||Recruiting||Concussion, Mild||Mayo Clinic|University of Minnesota - Clinical and Translational Science Institute|Thorne Research Inc.||September 2016||--|
|NCT02950441||Recruiting||Aging||University of Birmingham||June 2016||Early Phase 1|
|NCT02701127||Recruiting||Acute Kidney Injury||Beth Israel Deaconess Medical Center||March 2016||Early Phase 1|
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
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