Home DNA Damage/DNA Repair Sirtuin inhibitor Thiomyristoyl For research use only.

Thiomyristoyl

Synonyms: TM

Thiomyristoyl (TM) is a potent and specific SIRT2 inhibitor with an IC50 of 28 nM. It inhibits SIRT1 with an IC50 value of 98 μM and does not inhibit SIRT3 even at 200 μM.

Thiomyristoyl Chemical Structure

Thiomyristoyl Chemical Structure

CAS: 1429749-41-6

Selleck's Thiomyristoyl has been cited by 6 publications

Purity & Quality Control

Batch: S824501 DMSO] 100 mg/mL] false] Ethanol] 100 mg/mL] false] Water] Insoluble] false Purity: 99.84%
99.84

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Signaling Pathway

Sirtuin Signaling Pathways

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Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
BxPC3 Growth inhibition assay 72 hrs Growth inhibition of human BxPC3 cells after 72 hrs by CellTiter-Blue assay, GI50=13.3μM 30986062
MDA-MB-468 Growth inhibition assay 72 hrs Growth inhibition of human MDA-MB-468 cells after 72 hrs by CellTiter-Blue assay, GI50=15.7μM 30986062
NCI-H23 Growth inhibition assay 72 hrs Growth inhibition of human NCI-H23 cells after 72 hrs by CellTiter-Blue assay, GI50=16.4μM 30986062
HCT116 Function assay 5 days Inhibition of anchorage-independent cell growth in human HCT116 cells assessed as reduction in colony formation incubated for 5 days followed by compound re-addition and measured after 9 to 11 days by nitro blue tetrazolium chloride-based soft agar colony, GI50=16.7μM 30986062
A549 Growth inhibition assay 72 hrs Growth inhibition of human A549 cells after 72 hrs by CellTiter-Blue assay, GI50=17.3μM 30986062
SW948 Growth inhibition assay 72 hrs Growth inhibition of human SW948 cells after 72 hrs by CellTiter-Blue assay, GI50=19.2μM 30986062
MCF7 Growth inhibition assay 72 hrs Growth inhibition of human MCF7 cells after 72 hrs by CellTiter-Blue assay, GI50=37μM 30986062
MDA-MB-231 Growth inhibition assay 72 hrs Growth inhibition of human MDA-MB-231 cells after 72 hrs by CellTiter-Blue assay, GI50=42.8μM 30986062
HeLa Cytotoxicity assay 50 uM 72 hrs Cytotoxicity against human HeLa cells assessed as reduction in cell viability at 50 uM measured after 72 hrs by CCK8 assay 31117516
MCF7 Growth inhibition assay 15 uM 72 hrs Growth inhibition of human MCF7 cells at 15 uM measured after 72 hrs by AlamarBlue assay 31144814
Neuro2a Function assay 0.2 to 2 uM 24 hrs Induction of neurite outgrowth in mouse Neuro2a cells at 0.2 to 2 uM measured after 24 hrs by microscopic analysis 31144814
HCT116 Function assay 25 uM 6 hrs Inhibition of SIRT2 in human HCT116 cells assessed as increase in alpha-tubulin acetylation at 25 uM incubated for 6 hrs by DAPI staining-based laser scanning confocal microscopic analysis 30986062
HCT116 Function assay 50 uM 6 hrs Inhibition of SIRT2 in human HCT116 cells assessed as increase in alpha-tubulin acetylation at 50 uM incubated for 6 hrs by DAPI staining-based laser scanning confocal microscopic analysis 30986062
Click to View More Cell Line Experimental Data

Biological Activity

Description Thiomyristoyl (TM) is a potent and specific SIRT2 inhibitor with an IC50 of 28 nM. It inhibits SIRT1 with an IC50 value of 98 μM and does not inhibit SIRT3 even at 200 μM.
Targets
SIRT2 [1]
(Cell-free assay)
28 nM
In vitro
In vitro Thiomyristoyl(TM) is a highly selective SIRT2 inhibitor. It cannot efficiently inhibit SIRT3, SIRT5, SIRT6, or SIRT7. In vitro, it shows great inhibition of cell viability and its cytotoxicity is relatively selective toward cancer cells. TM decreases c-Myc oncoprotein level in cancer cells, the ability of TM to decrease c-Myc abundance in different cell lines correlates with the sensitivity of the cell lines to TM[1].
Cell Research Cell lines breast cancer cell lines MCF-7
Concentrations 1, 5, 10, 25, 50 μM
Incubation Time 6 h
Method Human MCF-7 cells are grown in DMEM media contained 10% (vol/vol) heat-inactivated fetal bovine serum and 1% penicillin-streptomycin and treated with in the presence of 200 nM TSA for 6 hr. The acetylation level of p53 protein is determined by western blot using anti-acetyl-p53 (K382) antibody. β-actin serves as a loading control.
In Vivo
In vivo The anticancer effect of TM correlates with its ability to decrease c-Myc level. TM has limited effects on non-cancerous cells and tumor-free mice[1].
Animal Research Animal Models Mouse xenograft model
Dosages 1.5 mg/50 μL (IP); 0.75 mg/50 μL (IT)
Administration intraperitoneal (IP) or intra-tumor (IT) injections
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06133153 Not yet recruiting
Lip; Anomaly
Croma-Pharma GmbH|Proinnovera GmbH
 March 2024 Not Applicable
NCT06147232 Not yet recruiting
Nephropathy|Diabetic Nephropathies|Diabetes Mellitus Type 1|Albuminuria|Diabetic Complications Renal|Diabetic Complications Cardiovascular|Hypoxia
Steno Diabetes Center Copenhagen|Juvenile Diabetes Research Foundation|King''s College London|Glostrup University Hospital Copenhagen
 February 2024 Phase 4
NCT05505643 Not yet recruiting
Breast Cancer|Breast Neoplasm
Washington University School of Medicine|The Foundation for Barnes-Jewish Hospital|Varian Medical Systems
 February 28 2024 Not Applicable
NCT05134740 Recruiting
Hodgkins Lymphoma|Non Hodgkins Lymphoma
Baylor College of Medicine|Center for Cell and Gene Therapy Baylor College of Medicine
 January 2024 Phase 1

Chemical Information & Solubility

Molecular Weight 581.85 Formula

C34H51N3O3S
CAS No. 1429749-41-6 SDF Download Thiomyristoyl SDF
Smiles CCCCCCCCCCCCCC(=S)NCCCCC(C(=O)NC1=CC=CC=C1)NC(=O)OCC2=CC=CC=C2
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 100 mg/mL ( (171.86 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 100 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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