Fludarabine

Catalog No.S1491 Synonyms: FaraA, Fludarabinum

Fludarabine Chemical Structure

Molecular Weight(MW): 285.23

Fludarabine is a STAT1 activation inhibitor which causes a specific depletion of STAT1 protein (and mRNA) but not of other STATs. Also a DNA synthesis inhibitor in vascular smooth muscle cells.

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  • ERK signaling regulates STAT1 phosphorylation, and pSTAT1 modulates MHC II expression in the spinal cord under BCP conditions. AG490 (5 μg in 10 μL), Fludarabine (10 μg in 10 μL), or U0126 (5 μg in 10 μL) was intrathecally injected into cancer-bearing rats once a day for 14 days, beginning immediately after carcinoma cell inoculation (n = 3 in each group). (A) Representative western blot showing pSTAT1ser727, total STAT1, pERK42/44, total ERK42/44, CIITA, MHC II RTIB, and b actin protein levels in the spinal cords of BCP rats.

    Brain Behav Immun, 2017, 60:161-173. Fludarabine purchased from Selleck.

    Imatinib mesylate (IM) in combination of fludarabine phosphate (F-AMP) significantly inhibits Ki67 and c-KIT expression in GIST-T1 tumor xenografts. Tumors were collected on the day after the last treatment and were then subjected to immunohistochemical detection of Ki67 and c-KIT expression. Representative images of immunohistochemical staining of Ki67 and c-KIT in mice tumors.

    Mol Cancer Ther, 2014, 13(10): 2276-87 . Fludarabine purchased from Selleck.

  • Normal human KC pretreated with STAT1 inhibitor (fludarabine [10 uM]) or STAT3 inhibitor (STA-21 [2 uM]) for 24 h. The mRNA levels of hBD2 and hBD3 were assessed by qRT-PCR.

    Mol Cell Biol 2014 34(24), 4368-78.. Fludarabine purchased from Selleck.

    Bacterial infection in IPEC-J2 cells. The invasion and attachment of EHECO157:H7 was increased in the IPEC-J2 cells in the presence of 10 μM fludarabine. Data are expressed as the mean ± SEM (n= 6). Differences between groups were determined by paired samples t-test. *P<0.05 compared with the control.

    Int Immunopharmacol, 2016, 36:199-204.. Fludarabine purchased from Selleck.

Purity & Quality Control

Choose Selective STAT Inhibitors

Biological Activity

Description Fludarabine is a STAT1 activation inhibitor which causes a specific depletion of STAT1 protein (and mRNA) but not of other STATs. Also a DNA synthesis inhibitor in vascular smooth muscle cells.
Targets
STAT1 [4]
(Vascular smooth muscle cells)
In vitro

Fludarabine efficiently inhibits the proliferation of RPMI 8226 cells with IC50 of 1.54 μg/mL. The IC50 of Fludarabine against MM.1S and MM.1R cells is 13.48 μg/mL and 33.79 μg/mL, respectively. In contrast, U266 cells are resistant to Fludarabine with IC50 of 222.2 μg/mL. Fludarabine treatment results in increased number of cells in the G1 phase of cell cycle, accompanied with a concomitant reduction of cells at the S phase of cell cycle in a time-dependent manner. Fludarabine induces a cell cycle block and triggers apoptosis in MM cells. Fludarabine triggers time-dependent cleavage of caspase-8, -9, and -3, -7, followed by PARP cleavage. Fludarabine increases expression of Bax in a time-dependent fashion, while the expression of Bak doesn't change. After exposure to Fludarabine for 12 hours, RPMI 8226 cells shows a loss of membrane potential with 61.05% of the cells expressing low fluorescence of rhodamine 123 compared with 8.62% of cells in untreated control. [1] To enhance solubility, Fludarabine is formulated as the monophosphate (F-ara-AMP, fudarabine), which is instantaneously and quantitatively dephosphorylated to the parent nucleoside upon intravenous infusion. Inside the cells rephosphorylation occurs which leads to fuoroadenine arabinoside triphosphate (F-ara-ATP), the major cytotoxic metabolite of F-ara-A. [2] Fludarabine can also induce pro-inflammatory stimulation of monocytic cells, as evaluated by increased expression of ICAM-1 and IL-8 release. [3] Fludarabine does not affect the growth of ovarian cancer cell lines, whereas it induces marked and dose-dependent inhibition of proliferation in melanoma cell lines. [4] Fludarabine induces significant reduction of STAT-1 phosphorylation, whereas it does not change JAK2 activation. Interestingly, Fludarabine does not significantly affect the phosphorylation of these three STAT proteins. Fludarabine (1.5 mg) significantly prevents STAT-1 phosphorylation and also reduces the increased amount of this protein. No significant changes are demonstrated in JAK2 phosphorylation at 2 days, but Fludarabine inhibits JAK2-increased expression at 7 days. Fludarabine specifically inhibits STAT-1 activation without affecting other STAT proteins and consequently diminishes VSMC proliferation. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Jeko-1  NGroU2NHfW6ldHnvckBCe3OjeR?= MWeyNEDPxE1? NUX2W41mOjRiaB?= M1zl[4lvcGmkaYTzJIV5eHKnc4Ppc44hd2ZiSVTP MmH6NlU6PDB5MUK=
MV-4-11 MVTBdI9xfG:|aYOgRZN{[Xl? MVGyMlUh|ryP NU\ERpJxPDhiaB?= M4jaSolv\HWlZYOgZZBweHSxc3nzJJNtcWeqdHz5 NV7CeFJtOjVzMUG1PFM>
THP-1 MoL6RZBweHSxc3nzJGF{e2G7 M33UeVIvPSEQvF2= M{PtNVQ5KGh? MmHjbY5lfWOnczDhdI9xfG:|aYOgd4xq\2i2bIm= NW\1SGE{OjVzMUG1PFM>
MOLM 13 NVHNRYhWSXCxcITvd4l{KEG|c3H5 M3LIUVIvPSEQvF2= M1XzTVQ5KGh? NHTEXm5qdmS3Y3XzJIFxd3C2b4Ppd{B{dGmpaITsfS=> MkXMNlUyOTF3OEO=
KBM3/Bu2506 NVHkXIpxSXCxcITvd4l{KEG|c3H5 M4fOTlIvPSEQvF2= NE\zVYQ1QCCq NVroR2xFcW6mdXPld{BieG:ydH;zbZMhe2yrZ3j0cJk> M1yxTlI2OTFzNUiz
Nalm-6 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3vxVWlEPTB;MUig{txO M4jzOFI2ODZzMUCx
Reh M2L5Umdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M17zT2lEPTB;M{Cg{txO NX3mUXZQOjVyNkGxNFE>
U2937 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1TJ[GlEPTB;MU[g{txO MnHkNlUxPjFzMEG=
Mec-1 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXPJR|Ux97zgNUCwJO69VQ>? M{nCPVI2ODZzMUCx
RPMI-8226 NVjxOGs5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWr2eoJZUUN3ME21NFAh|ryP MkK5NlUxPjFzMEG=
Molt-4 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{jvOWlEPTB;MUiwJO69VQ>? MlHXNlUxPjFzMEG=
Nalm-6-FluR MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnS5TWM2OD1{NUCg{txO MVyyOVA3OTFyMR?=
Raji  MmLVSpVv[3Srb36gRZN{[Xl? MV:zxsDPxE1? Ml3pNlQwPDhxN{KgbC=> NGT6[XdqdmS3Y3XzJIFk[3WvdXzheIlwdnNib3[gdFU{NCCyNkOgZY5lKHB5M9Mg MYmyOFk1ODZ7NR?=
PBMC NHr6UFBHfW6ldHnvckBCe3OjeR?= MlHTOVAwOTByIN88US=> NYXtVWprOjRiaB?= NGjWR5ZFVVOR MX;pcohq[mm2czDTWGFVOSCyaH;zdIhwenmuYYTpc44> NILEbWszPDlzMUi3Ni=>
MDA-231 MkDnSpVv[3Srb36gRZN{[Xl? M2rBdlExOCEQvF2= M1G2OVI1KGh? NFLJcZpFVVOR M1nyR4Rm[3KnYYPld{BKTE9iZYjwdoV{e2mxbh?= NGnmNnIzPDlzMUi3Ni=>
624.38mel  NYG3PVI4TnWwY4Tpc44hSXO|YYm= NYf6SGg6PTBizszN MkPGNlQhcA>? NYXHVlVYTE2VTx?= M{jGeIRm[3KnYYPld{BKTE9iZYjwdoV{e2mxbh?= NYG4UoxYOjR7MUG4O|I>
MDA-231 M{HNTmZ2dmO2aX;uJGF{e2G7 M1mwVFUxNTJyMDFOwG0> Ml76NlQhcA>? MkDsSG1UVw>? MmL2bY5pcWKrdIOgTWRQKGGldHn2bZR6KGmwZHXw[Y5l\W62bImgc4YhdVKQQTDs[ZZmdHN? M3LQc|I1QTFzOEey
624.38mel  M1\jW2Z2dmO2aX;uJGF{e2G7 M4nIZ|UxNTJyMDFOwG0> M2\BUVI1KGh? M1\QOGROW09? MV3pcohq[mm2czDJSG8h[WO2aY\peJkhcW6mZYDlcoRmdnSueTDv[kBuWk6DIHzleoVtew>? NF\CeHozPDlzMUi3Ni=>
HMECs M{TCZ2Z2dmO2aX;uJGF{e2G7 NIH4U4YyODEEoN88UeKh MV6zOuKhcA>? NVy2O5hFcW6qaXLpeJMhUU[QzsRCpIFv\CCOUGOgbY5lfWOnZDDTWGFVOSCyaH;zdIhwenmuYYTpc44h[W6mIFnSSlEh\XiycnXzd4lwdg>? NE\mN5YzPDJzMUOyOy=>
HMECs  MVHGeY5kfGmxbjDBd5NigQ>? MoWzNVAxyqEQvF5CpC=> NVf4boJjOzcEoHi= Mn2zbY5pcWKrdIOgTWZP|rIEoH3l[IlifGWmIIDoc5NxcG:{eXzheIlwdiCxZjDTWGFVOSCjbnSgV3RCXDNuIHL1eEBvd3Rib3[gV3RCXDJ? MnzTNlQzOTF|Mke=
BJAB M2e2eGFxd3C2b4Ppd{BCe3OjeR?= NHi2Wmg2yqEQvF2= NI\T[nMzPCCq NVP0[3RGcW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?= NIrYXoszPDB3N{G0Oy=>
I-83 NFHpd2ZCeG:ydH;zbZMhSXO|YYm= NHHtOYY2yqEQvF2= M2nTc|I1KGh? NIO2W5FqdmS3Y3XzJINmdGxiYYDvdJRwe2m| M2XvbFI1ODV5MUS3
NALM6 NF:4UFhCeG:ydH;zbZMhSXO|YYm= Ml3zOeKh|ryP MmTINlQhcA>? NGDrem5qdmS3Y3XzJINmdGxiYYDvdJRwe2m| NH7jU2szPDB3N{G0Oy=>
DU-145 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{\pbVAuOTBizsznM41t MnjIOFghcMLi NHLSSWxqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? MYSyN|c{PDhzNR?=
Nalm-6 NWHGcVh6TnWwY4Tpc44hSXO|YYm= MV:xNOKh|ryP NH\BUmQyNzJxNDDo MUjpcoR2[2W|IHH1eI9xcGGpeR?= NEnSUlczOzZ6MUKyNy=>
Reh NWrOU4s{TnWwY4Tpc44hSXO|YYm= NYPNXWI4OTEEoN88US=> MkfJNU8zNzRiaB?= M4nLUYlv\HWlZYOgZZV1d3CqYXf5 NIjvcHkzOzZ6MUKyNy=>
Nalm-6 NHXtTopIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIqzfGZKSzVyIPMIwFEx6oDLzszN NXLQZY1bOjN4OEGyNlM>
Reh NEK1dJRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoPzTWM2OCEkiMyxNQKBkc7:TR?= M3\NPVI{PjhzMkKz
HEC1A MnrHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3HvNlExOC13MECg{txO MljHNlQhcA>? MUDpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> MV6yN|U6PTZ7Nx?=
AN3CA Ml;2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUexNFAuPTByIN88US=> NYTBZ4xwOjRiaB?= MUfpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NHPSSIszOzV7NU[5Oy=>
HEC50B MkLUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnqwNVAxNTVyMDFOwG0> M2jtelI1KGh? NHu0ZWVqdmirYnn0d{Bk\WyuIHfyc5d1cCC|bHnnbJRtgQ>? MYmyN|U6PTZ7Nx?=
HEC1A MnPnRZBweHSxc3nzJGF{e2G7 MUmyNE8yODBizszN M4XLVlI1KGh? MXTpcoR2[2W|IHHwc5B1d3OrczDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= NHXSWJYzOzV7NU[5Oy=>
AN3CA NVXkVXo1SXCxcITvd4l{KEG|c3H5 M2jjTVIxNzFyMDFOwG0> Ml65NlQhcA>? NF:1eHdqdmS3Y3XzJIFxd3C2b4Ppd{BqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NIW5SZczOzV7NU[5Oy=>
HEC50B M1rmc2Fxd3C2b4Ppd{BCe3OjeR?= MnnzNlAwOTByIN88US=> M4HYTlI1KGh? MXXpcoR2[2W|IHHwc5B1d3OrczDzcIlocHSueR?= NEDPZWkzOzV7NU[5Oy=>
EHEB NHj4eIZCeG:ydH;zbZMhSXO|YYm= NEX1[ZA1OCEQvF2= M4HkUVI1KGh? M2DC[4lv\HWlZYOgZZBweHSxc3nz MkjxNlM1QTdyN{W=
A549 NEnscJVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYD3d4lRUUN3ME2xOU44yrF{LkigxtVO NIHOWFQzOzN5N{G5Ni=>
A549 GAPDH-deficient MlW2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4n5SGlEPTB;MUiuOeKyOi5|INM1US=> NXLZdVY2OjN|N{exPVI>
CLL  MknqRZBweHSxc3nzJGF{e2G7 MnrhNVAh|ryPwrC= Mn;4NlQuQTZiaB?= MU\pcoR2[2W|IHHwc5B1d3SrYzDj[YxtKGSnYYTo NWDWU4FKOjJ{MEe2PFY>
MEC1 M{C4emFxd3C2b4Ppd{BCe3OjeR?= MU[xNFDDqM7:TR?= M4\vNVczKGh? Ml7DbY5lfWOnczDhdI9xfG:|aYOgd4lodmmoaXPhcpRtgQ>? NUPGcWZ4OjJzM{K5O|M>
U937  MnXyRZBweHSxc3nzJGF{e2G7 MkfDNE45KM7:TR?= NF\RcYI1NTR6IHi= MVjpcoR2[2W|IHHwc5B1d3OrczDzcIlocHSueR?= M{DZ[lIzODd2N{Cw
U937  M37lNGFxd3C2b4Ppd{BCe3OjeR?= NFnxNFEyKM7:TR?= NGTVTVM6PiCq NUTHOIZzcW6mdXPld{BieG:ydH;zbZMhe2yrZ3j0cJk> M3Ti[VIzODJ|NUKz
Daudi NUXwRXpMSXCxcITvd4l{KEG|c3H5 NIPjdGIzOCEQvF2= MnjlPVYhcA>? NUXhPWhbcW6mdXPld{BieG:ydH;zbZMhe2yrZ3j0cJk> NF3qVVIzOjB{M{WyNy=>
J45.01 NVHBS5BrSXCxcITvd4l{KEG|c3H5 NEPxN|IyKM7:TR?= NIXwWGM6PiCq NXnEPXM2cW6mdXPld{BieG:ydH;zbZMhe2yrZ3j0cJk> MnPsNlIxOjN3MkO=
RPMI 8226 NYDxVJBUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkfLTWM2OD1{NT65xsDDucLiMz63JO69VQ>? MoTYNlE6PDh{NkS=
CEM NH;BWI1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NInKc2ZKSzVyPUKuOOKhyrIEoECuOEDPxE1? M{fIZlIyQTR6Mk[0
Raji MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV7JR|UxRTBwNEhCpOKyyqByLkC0JO69VQ>? NGTjTm4zOTl2OEK2OC=>
U937 NVzZUGl2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml\iTWM2OD1yLkK0xsDDucLiMD6wOEDPxE1? NHH6bmczOTl2OEK2OC=>
K562 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MojwTWM2OD1yLkS0xsDDucLiMD6wOUDPxE1? NGXvUmgzOTl2OEK2OC=>
NALM-6 NHL3cmxCeG:ydH;zbZMhSXO|YYm= M4XBc|ExKM7:TdMg M{H0V|I1KGh? M1z0XIlv\HWlZYOgZ4VtdCCjcH;weI9{cXNic3zp[4h1dHl? NXX3WIdjOjF4OUmzPFM>
JMV-3 NXnEUIhQSXCxcITvd4l{KEG|c3H5 MWSxNEDPxE4EoB?= NFHz[XozPCCq NGLYSYRqdmS3Y3XzJINmdGxiYYDvdJRwe2m|IIPsbYdpfGy7 MkTUNlE3QTl|OEO=
EHEB MVrGeY5kfGmxbjDBd5NigQ>? NI\mWZM2NTVyIN88US=> NIKxbJIzPCCq MW\k[YNz\WG|ZYOgdFIyKGW6cILld5Nqd25ic3nncolncWOjboTsfS=> MoPoNlEyPjh|OUG=
JVM-2  NEG2TWRHfW6ldHnvckBCe3OjeR?= NVT0flZJOzBizszN MmLGNlQhcA>? NH\qXHhl\WO{ZXHz[ZMheDJzIHX4dJJme3Orb36= MX2yNVE3QDN7MR?=
KBM3/Bu2506 MmDmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUTJR|IxRTBwNkegxtVO NEDQOXEzODl|M{WwPS=>
KBM3/Bu2506 M{HxVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV2zV|NOOC54IN88US=> NVzMSWxXOjRiaB?= M4DlUolv[3KnYYPld{B1cGViY3XscEBnemGldHnvckBqdiCVLYDoZZNm NILUTFIzODl|M{WwPS=>
MDA-MB-231 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1XVeGlEPTB;ND6wJO69VQ>? MXWyNFQ1PzN7MB?=
MCF-7 M3izd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXjFT|Q{UUN3ME2xOU4xKM7:TR?= MWGyNFQ1PzN7MB?=
HLE-B3  NGXIOoFHfW6ldHnvckBCe3OjeR?= M1PnelI2KM7:TR?= Mk[0OFghcA>? NX3hRWR{[myxY3vzJGlHVi4Qs,MAl4lv\HWlZXSgV3RCXDFicHjvd5Bpd3K7bHH0bY9vKGGwZDDJSG8h\XiycnXzd4lwdg>? MWSyNFQ{PTF3OB?=
K562 MoHxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYLqOWl1PzJiaB?= NXr0PXR[UUN3ME2zMlMhdk1? M4HWWlIxOzB5MUm4
BW-225 MkDJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{fNfWlEOjB;MT6zO{DEnzFy4pkSPOKh|ryPwrC= NYrwe|FPOTh4NkGzPFA>
OH-65 MmjmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2n6cGlEOjB;MT6zO{DEnzFy4pkSPOKh|ryPwrC= Mn3WNVg3PjF|OEC=
GR-145 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWLJR|IxRTJwN{Sgx7chOTEkiKK4JEDPxE4EoB?= M13uZ|E5PjZzM{iw
A549 Mkn5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF33NHRKSzJyPUWuOFghy5diMUFijLI5KM7:TdMg NWXqO|J6OTh4NkGzPFA>
CaSki  Mn7YS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYHJR|IxRTFwM{egx7chOTEkiKK3JO69VcLi Ml;rNVg3PjF|OEC=
ZMK-1 NXnaTm5IT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH7vUldKSzJyPUGuN|chy5diMUFijLI3KM7:TdMg NFfycHEyQDZ4MUO4NC=>
SKW6.4 MVfBdI9xfG:|aYOgRZN{[Xl? NEP0d4ExNjBzLUGwJO69VQ>? NX3ye2dqOjRxNEigbC=> NGC4e2JqdmS3Y3XzJINmdGxiZHXheIghcW5iYn;0bEB1cW2nLTDhcoQh\G:|ZT2g[IVx\W6mZX70JI1idm6nch?= MXSxPFA6OjN2MB?=
RPMI 8226 NYj4b4R5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4LqPFI1KGh? NHntO4hKSzVyPUGuOVTDqM7:TR?= MVixO|k4PjF6Nh?=
MM.1S M4ToT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEe2[oI1QCCq NIDIOpNKSzVyPUGzMlQ5yqEQvF2= NGD2Z3AyPzl5NkG4Oi=>
MM.1R M2\LVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHy5OnI1QCCq MWHJR|UxRTN|Lke5JO69VQ>? M3HQPFE4QTd4MUi2
U937 M3L6O2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2LyZWlEPTB;MzyyNFAhyrFiNU[wJI5O Mn;FNVU6OzB|NkG=

... Click to View More Cell Line Experimental Data

In vivo Tumors treated with PBS grow rapidly to approx-imately 10-fold their initial volume in 25 day, whereas, the tumors in the Fludarabine at 40 mg/kg increase less than 5-fold. A significant antitumor effect of 40 mg/kg Fludarabine on RPMI8226 tumor growth is demonstrated. RPMI8226 tumors treated with 40 mg/kg Fludarabine at day 10 increase apoptotic nuclei. Fludarabine is effective in suppressing RPMI8226 myeloma xenografts in SCID mice. [1]

Protocol

Cell Research:

[1]

+ Expand
  • Cell lines: Dexamethasone-sensitive (MM.1S) and -resistant (MM.1R) human MM cell lines, RPMI8226 and U266 cell lines
  • Concentrations: 2 μg/mL
  • Incubation Time: 24 hours
  • Method:

    After treated with Fludarabine or control, dexamethasone-sensitive (MM.1S) and -resistant (MM.1R) human MM cell lines, RPMI8226 and U266 cell lines (5 × 105 cells) are washed twice in phosphate-buffered saline (PBS) and fixed with 70% ice-cold ethanol, then centrifuged and suspended in PBS containing 100 μg/mL RNase A. After incubated for 30 minutes at 37 ºC, samples are resuspended in 25 μg/mL propidium iodide. Flow cytometry is performed on a FACSCalibur automated system. Apoptosis is determined by Annexin V-FITC apoptosis detection kit, according to the manufacturer's instructions. For TUNEL (terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling) assay, cells are analyzed by flow cytometry using the in situ cell death detection kit.


    (Only for Reference)
Animal Research:

[1]

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  • Animal Models: Severe combined immunodeficient (SCID) mice bearing RPMI 8226 cells
  • Formulation: PBS
  • Dosages: 40 mg/kg
  • Administration: Administered via i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 57 mg/mL (199.83 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents individually and in order:
30% propylene glycol, 5% Tween 80, 65% D5W
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 285.23
Formula

C10H12FN5O4

CAS No. 21679-14-1
Storage powder
in solvent
Synonyms FaraA, Fludarabinum

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01585415 Terminated Metastatic Cancer|Melanoma National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) April 9, 2012 Phase 1
NCT01503242 Recruiting Plasma Cell Myeloma|Refractory Plasma Cell Myeloma Fred Hutchinson Cancer Research Center|National Cancer Institute (NCI) January 9, 2012 Phase 1
NCT01319565 Active, not recruiting Metastatic Melanoma|Skin Cancer National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) March 9, 2011 Phase 2
NCT01145209 Active, not recruiting Small Lymphocytic Lymphoma|CLL (Chronic Lymphocytic Leukemia) National Heart, Lung, and Blood Institute (NHLBI)|University of Virginia|GlasoSmithKline|National Institutes of Health Clinical Center (CC) June 9, 2010 Phase 2
NCT02500576 Recruiting Melanoma M.D. Anderson Cancer Center|Merck Sharp & Dohme Corp.|Prometheus Inc. August 7, 2015 Phase 2
NCT01174121 Recruiting Metastatic Colorectal Cancer|Metastatic Gastric Cancer|Metastatic Pancreatic Cancer|Metastatic Hepatocellular Carcinoma|Metastatic Cholangiocarcinoma National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) July 7, 2010 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID