Afatinib (BIBW2992)

Catalog No.S1011

Afatinib (BIBW2992) Chemical Structure

Molecular Weight(MW): 485.94

Afatinib (BIBW2992) irreversibly inhibits EGFR/HER2 including EGFR(wt), EGFR(L858R), EGFR(L858R/T790M) and HER2 with IC50 of 0.5 nM, 0.4 nM, 10 nM and 14 nM in cell-free assays, respectively.

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In DMSO USD 240 In stock
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USD 210 In stock
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Cited by 37 Publications

4 Customer Reviews

  • Cytoxicity of irreversible EGFR TKIS and ethacrynic acid on breast cells lines. A, Mean IC50 value of Afatinib.

    Oncotarget, 2016, 7(36):58038-58050. Afatinib (BIBW2992) purchased from Selleck.

    Inhibition of signaling pathway activation in lung tumor cell lines by kinase inhibitors. Lung tumor cells were cultured in 10% FBS until reaching ∼80% confluence and then the cells were starved in serum-free medium for overnight, followed by 4-hour treatment with the inhibitors. Cell lysates were then prepared and used for determination of the pathway activation signals by the CEER assay.

    Int J Proteomics 2011 2011, 215496. Afatinib (BIBW2992) purchased from Selleck.

  • Inhibition of anchorage-independent growth of lung tumor cell lines by selected inhibitors. Each selected cell line was treated with the indicated inhibitor at 0.1 μM and 1 μM concentrations for two weeks and cell colony size formation was scored under the Nikon inverted-phase microscope.

    Int J Proteomics 2011 2011, 215496. Afatinib (BIBW2992) purchased from Selleck.

    After starved in serum-free medium for 24h, Breast cancer cells incubated with the indicated concentrations of BIBW2992 for 3h,followed by 15-minute stimolation of 100ng/ml EGF

    Dr. Zhang of Tianjin Medical University. Afatinib (BIBW2992) purchased from Selleck.

Purity & Quality Control

Choose Selective EGFR Inhibitors

Biological Activity

Description Afatinib (BIBW2992) irreversibly inhibits EGFR/HER2 including EGFR(wt), EGFR(L858R), EGFR(L858R/T790M) and HER2 with IC50 of 0.5 nM, 0.4 nM, 10 nM and 14 nM in cell-free assays, respectively.
Targets
EGFR (L858R) [1]
(Cell-free assay)
EGFR (wt) [1]
(Cell-free assay)
EGFR (L858R/T790M) [1]
(Cell-free assay)
HER2 [1]
(Cell-free assay)
0.4 nM 0.5 nM 10 nM 14 nM
In vitro

BIBW2992 shows potent activity against both wild-type and mutant forms of EGFR and HER2. It is similar to Gefitinib in potency against L858R EGFR, but about 100-fold more active against the Gefitinib resistant L858R-T790M EGFR double mutant. BIBW2992 exhibits potent effects on both EGFR and HER2 phosphorylation in vivo. It compares favorably to reference compounds (such as Lapatinib et al.) in all cell types tested, such as human epidermoid carcinoma cell line A431 expressing wt EGFR, murine NIH-3T3 cells transfected with wt HER2, as well as breast cancer cell line BT-474 and gastric cancer cell line NCI-N87, which express endogenous HER2. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HDLM-2 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVTJR|UxRTBwMEC1O|Ih|ryP MmniV2FPT0WU
KASUMI-1 NVLYenlOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX3wbXhZUUN3ME2wMlAxQTFzIN88US=> NYjSZVAxW0GQR1XS
HCC2218 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{m2WGlEPTB;MD6wNFk5PyEQvF2= MWjTRW5ITVJ?
KARPAS-45 NY\YXWRiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV3JR|UxRTBwMEG2O|Eh|ryP MYjTRW5ITVJ?
HSC-4 NITKblRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV\JR|UxRTBwMEG3OFgh|ryP NUK0WIVKW0GQR1XS
CHL-1 MoDUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX\RVZZ5UUN3ME2wMlAyQDN7IN88US=> NYj3PYt6W0GQR1XS
KY821 NI\DR|RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV;vcXR4UUN3ME2wMlAzODZizszN MkW3V2FPT0WU
NCI-N87 NYTGOphJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF\lUWlKSzVyPUCuNFI4PzNizszN MlLPV2FPT0WU
DOK MkeyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml;PTWM2OD1yLkCzNVk1KM7:TR?= MWrTRW5ITVJ?
DSH1 MnXMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV;UPVlpUUN3ME2wMlA{OzF2IN88US=> M4f6dnNCVkeHUh?=
MDA-MB-175-VII NF[1XI5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{fySmlEPTB;MD6wN|QyPiEQvF2= NXj6R|ZrW0GQR1XS
NCI-H2170 M3XKbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYrEeGx2UUN3ME2wMlA{PzJ6IN88US=> Mn\TV2FPT0WU
BT-474 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXPJR|UxRTBwMES5PFYh|ryP NWC1TY4zW0GQR1XS
EFO-27 NFXUc2hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV;JR|UxRTBwMEWxPFIh|ryP NHnrR|JUSU6JRWK=
NCI-H720 Mo\DS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWDJR|UxRTBwMEWzNlUh|ryP MW\TRW5ITVJ?
PC-14 NXWzOGhYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYjJR|UxRTBwMEW2Olgh|ryP MUfTRW5ITVJ?
EC-GI-10 M1LjTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MknXTWM2OD1yLkC1PVEzKM7:TR?= Mn;YV2FPT0WU
KYSE-450 MkTaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M171WGlEPTB;MD6wOVk4QCEQvF2= NX3nVWdRW0GQR1XS
BPH-1 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NED1NXRKSzVyPUCuNFU6QDhizszN MVTTRW5ITVJ?
NCI-H292 NIPDelNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYfJR|UxRTBwME[wPUDPxE1? NX\ZPVFbW0GQR1XS
H9 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUPic4loUUN3ME2wMlA4OTN{IN88US=> M{foN3NCVkeHUh?=
NCI-H526 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYrJR|UxRTBwMEe4NlMh|ryP MmnTV2FPT0WU
NCI-H1623 NIXBXolIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXLRPXJjUUN3ME2wMlA5ODJ3IN88US=> MWXTRW5ITVJ?
KYSE-140 NEjWdodIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mm\yTWM2OD1yLkC4N|Mh|ryP MWrTRW5ITVJ?
Ca9-22 M3\rTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWPZfmVHUUN3ME2wMlA5OzV|IN88US=> NHvGeItUSU6JRWK=
AU565 M{L4XGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnjITWM2OD1yLkC4OVA1KM7:TR?= MnPIV2FPT0WU
ZR-75-30 M2\OXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mo\uTWM2OD1yLkC5OVQ2KM7:TR?= MY\TRW5ITVJ?
NCI-H1770 NXrqZZhvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWfJR|UxRTBwMUCyPEDPxE1? NUXJZlAyW0GQR1XS
LB2241-RCC M1e1Vmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVfGNGYxUUN3ME2wMlExQDh2IN88US=> NYS4V5VlW0GQR1XS
CAL-39 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGfN[lBKSzVyPUCuNVE4QTJizszN NH\4[HZUSU6JRWK=
UACC-893 M1zLbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3PQXWlEPTB;MD6xNVg5PSEQvF2= NGLSXFdUSU6JRWK=
LU-65 NETYVFlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2iySWlEPTB;MD6xNlY{OyEQvF2= MmW3V2FPT0WU
HN Mo\SS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHHpPHhKSzVyPUCuNVMxPzdizszN Mof6V2FPT0WU
TI-73 NYWzTVNyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUi0VW5HUUN3ME2wMlE1PzF3IN88US=> NYXoOJlQW0GQR1XS
KU812 NX;p[mRvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mln0TWM2OD1yLkG1OFU3KM7:TR?= NFu1O5pUSU6JRWK=
SW13 NV\PS2FXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXPJR|UxRTBwMU[yNVYh|ryP NFrjbHFUSU6JRWK=
BB30-HNC NXLke49kT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYnsS2ozUUN3ME2wMlE3PTd4IN88US=> MkXEV2FPT0WU
HSC-2 NYr6[lA6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV;JR|UxRTBwMU[4N|ch|ryP NHrzZWxUSU6JRWK=
BV-173 NYn6cllOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHz2UW5KSzVyPUCuNVc2QTZizszN NHzGb2RUSU6JRWK=
CAL-27 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoXTTWM2OD1yLkG3OlM3KM7:TR?= Mn;jV2FPT0WU
SBC-1 M1;vTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkfiTWM2OD1yLkG4PVU6KM7:TR?= NWnDSWE1W0GQR1XS
CAL-33 NES4O2VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{DpUGlEPTB;MD6yNFE5PCEQvF2= MlfCV2FPT0WU
NCI-H209 M13KRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnjpTWM2OD1yLkKwOFYh|ryP M{fES3NCVkeHUh?=
COR-L105 M361bmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NI\1THpKSzVyPUCuNlExQDRizszN NXXINphJW0GQR1XS
HSC-3 NE\RcZNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlL3TWM2OD1yLkKxNVk5KM7:TR?= NWnYNJV3W0GQR1XS
NCI-H69 M2D4e2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUPtXYR3UUN3ME2wMlIyPDVzIN88US=> M3nVPHNCVkeHUh?=
MOLT-13 MkPGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVrJR|UxRTBwMkO5O|Uh|ryP NInOZZhUSU6JRWK=
ML-2 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWDZXGRDUUN3ME2wMlI1QDB{IN88US=> Ml3MV2FPT0WU
IGROV-1 MmfIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MljkTWM2OD1yLkK1PVUzKM7:TR?= NYP1[YI2W0GQR1XS
TK10 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1nre2lEPTB;MD6yOlc{QCEQvF2= M3LuXnNCVkeHUh?=
EoL-1-cell M1XOUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYLJR|UxRTBwMk[4PVIh|ryP NYGwVGZKW0GQR1XS
HO-1-N-1 NYHwUIlqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmHjTWM2OD1yLkK3PFU{KM7:TR?= MV7TRW5ITVJ?
BHY M2DTemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{PtU2lEPTB;MD6yPFY2PSEQvF2= M{fMPXNCVkeHUh?=
LU-139 M4PjUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHO3VJVKSzVyPUCuN|A2OSEQvF2= M1PQfXNCVkeHUh?=
23132-87 MnHHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVzJR|UxRTBwM{G5NlMh|ryP NFjIdHBUSU6JRWK=
ACHN NW\pPGF4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWXiZ5hFUUN3ME2wMlMyQTl2IN88US=> MWHTRW5ITVJ?
DoTc2-4510 NVq5VZM1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX\xSJZuUUN3ME2wMlMzOjF7IN88US=> NHfh[4xUSU6JRWK=
BB65-RCC MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXXJR|UxRTBwM{S1PVMh|ryP MoWwV2FPT0WU
HLE M1P6W2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYDJR|UxRTBwM{WwOFYh|ryP NHLUXIxUSU6JRWK=
HCC1419 NH[3PZZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEfUPZRKSzVyPUCuN|Y1ODhizszN MV7TRW5ITVJ?
A388 Mm\YS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFfxWopKSzVyPUCuN|Y5PTdizszN NEjrWWJUSU6JRWK=
NUGC-3 NGnHeJJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHm4RVNKSzVyPUCuN|g{PzNizszN MVLTRW5ITVJ?
KYSE-180 MkLaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGXPdmZKSzVyPUCuN|kxQDlizszN MlnqV2FPT0WU
TE-6 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mlu3TWM2OD1yLkSwNlQ3KM7:TR?= MlHPV2FPT0WU
A498 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH[4c4FKSzVyPUCuOFA3PzRizszN Ml24V2FPT0WU
NCI-H1648 M1jvOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NULib21zUUN3ME2wMlQ1OTh{IN88US=> NV3oN|R6W0GQR1XS
DU-145 NFHsflZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4HVdWlEPTB;MD60OFg{OyEQvF2= MWTTRW5ITVJ?
CW-2 NHfUcJdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEHHU2tKSzVyPUCuOFY6OTRizszN M4XS[nNCVkeHUh?=
A431 Mn3oS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVXTZmRrUUN3ME2wMlQ5OjN|IN88US=> MVHTRW5ITVJ?
SAS MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV7JR|UxRTBwNEizPVgh|ryP NX7wSFh3W0GQR1XS
NCI-H2126 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3K2[GlEPTB;MD61NVkzPiEQvF2= NY\TUlBRW0GQR1XS
NCI-H358 NH;SSmNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NULKdYdpUUN3ME2wMlU{OjFizszN MUnTRW5ITVJ?
FADU NUnsTZE5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUnJR|UxRTBwNUS0NFQh|ryP MmLKV2FPT0WU
ACN NWXQZ4x{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVfSZ3RmUUN3ME2wMlU2ODl2IN88US=> NYHlTIFMW0GQR1XS
HCC70 NFzYRVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYDJR|UxRTBwNUe2OlEh|ryP NHjJUYRUSU6JRWK=
NCI-H1838 NGj2dotIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGnQR4NKSzVyPUCuOlAzQTlizszN M4jTUHNCVkeHUh?=
CAL-54 NUTEfnBFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2LYNmlEPTB;MD62NVYzOSEQvF2= MXPTRW5ITVJ?
NCI-H1975 Mny2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NI\6UGpKSzVyPUCuOlE4OjhizszN MUPTRW5ITVJ?
COR-L88 NIq1OIVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWTJR|UxRTBwNkSxNVMh|ryP NH3YVI5USU6JRWK=
CTV-1 MonBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXHJR|UxRTBwNkSzPVIh|ryP MWLTRW5ITVJ?
EW-18 NWfNW5BXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2HVPWlEPTB;MD62PFY6QCEQvF2= MoLHV2FPT0WU
HCC1806 NIPxS2dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MW\JR|UxRTBwNkm2OlYh|ryP MVjTRW5ITVJ?
HuP-T4 MkHHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIXUfXJKSzVyPUCuOlk4PiEQvF2= MlzhV2FPT0WU
MDA-MB-361 M2G4c2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGfHPWtKSzVyPUCuO|I3PjJizszN MkSxV2FPT0WU
NCI-H1573 NHLFTVlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2HDVmlEPTB;MD63N|Y{KM7:TR?= MWPTRW5ITVJ?
KU-19-19 NHPNboRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV3JR|UxRTBwN{e0OlQh|ryP MXXTRW5ITVJ?
COLO-678 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlP5TWM2OD1yLkiwPFM1KM7:TR?= NWPYR4hmW0GQR1XS
C-33-A MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnXnTWM2OD1yLkixNlk4KM7:TR?= MYLTRW5ITVJ?
Calu-3 M{W4eWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml3xTWM2OD1yLkixOFM1KM7:TR?= NXewd5dlW0GQR1XS
AGS M1LiUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYrwfnkxUUN3ME2wMlg{PjR6IN88US=> NVHieFJIW0GQR1XS
SCC-25 NX\R[|FJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYDJR|UxRTBwOESxO|Eh|ryP MoPUV2FPT0WU
JVM-2 MnH1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVzzfHcxUUN3ME2wMlg5Ojl7IN88US=> NHvzZolUSU6JRWK=
HCE-T M2ix[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{\6SWlEPTB;MD64PFQzQCEQvF2= MnLNV2FPT0WU
ES4 NWK0OJZsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVHhSW1uUUN3ME2wMlk4QDN4IN88US=> M3PURnNCVkeHUh?=
EKVX MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV3FNFZRUUN3ME2xMlAyODZ2IN88US=> NUfiO|VkW0GQR1XS
D-542MG MlfrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYjJR|UxRTFwMEGwOlQh|ryP NXn1[HhUW0GQR1XS
SW1116 M{XkWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4XnUmlEPTB;MT6wNVI5PyEQvF2= NX;MU4tWW0GQR1XS
5637 MlW5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFS0[JpKSzVyPUGuNFE2OSEQvF2= M4DJPXNCVkeHUh?=
HT-1376 Mn7RS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUDmXlVoUUN3ME2xMlAyPTF|IN88US=> MV\TRW5ITVJ?
NB69 MnjVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkTWTWM2OD1zLkCyPVQ2KM7:TR?= MVPTRW5ITVJ?
YKG-1 NHjaSIxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWP0R2pDUUN3ME2xMlA2PDF7IN88US=> NGGzdG5USU6JRWK=
EW-3 Mnr2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVfJR|UxRTFwMEe4PFMh|ryP MlrNV2FPT0WU
GCIY MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NETsN41KSzVyPUGuNFgyODRizszN MUDTRW5ITVJ?
SK-MES-1 Mne2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWP5WGE6UUN3ME2xMlEyPDh5IN88US=> MmjNV2FPT0WU
PANC-08-13 NGfm[2RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3v1cWlEPTB;MT6xOlQyPiEQvF2= MXXTRW5ITVJ?
SK-NEP-1 NWjKUXFpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUPJR|UxRTFwMUe1NVMh|ryP NWXoV24{W0GQR1XS
SW954 M{\3bWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MorXTWM2OD1zLkKwNlE1KM7:TR?= M{PSfXNCVkeHUh?=
NCI-SNU-5 NWrUfHJQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3n4PWlEPTB;MT6yN|kh|ryP MlvoV2FPT0WU
SW48 NEDpfHpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHrKdY9KSzVyPUGuNlU1OzJizszN NE\xV4hUSU6JRWK=
HT-3 NUnreoRnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF3yUnlKSzVyPUGuNlg4PTNizszN Mmq1V2FPT0WU
ETK-1 NUPNPGhFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHPwfVFKSzVyPUGuN|c1PTZizszN MXzTRW5ITVJ?
SCC-15 NF3NbXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWHmeVROUUN3ME2xMlM6PDVzIN88US=> M4rQNHNCVkeHUh?=
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LB373-MEL-D Mo[zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXTqN5dYUUN3ME23Mlc3PzdzIN88US=> MnXOV2FPT0WU
SW900 MknxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUjOT2tiUUN3ME23MlgzOTJzIN88US=> MXLTRW5ITVJ?
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HAL-01 MnX6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXT6Zm53UUN3ME24MlUyQTFizszN MoTaV2FPT0WU
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OCI-AML2 NHriOHVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4TVfmlEPTB;OD65OVQ5OSEQvF2= M3OzT3NCVkeHUh?=
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NCI-H1703 MkLiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1O2WmlEPTB;OT6zOFM4PSEQvF2= M1HmRnNCVkeHUh?=
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NOS-1 MnPqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4TMUmlEPTB;MUGuO|ExQSEQvF2= M{jRcXNCVkeHUh?=
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NCI-H596 NEWz[IxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NELkSoFKSzVyPUGyMlA{PjlizszN MVPTRW5ITVJ?
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OVCAR-5 MnroS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MonUTWM2OD1zMz6xOVk{KM7:TR?= M1HTd3NCVkeHUh?=
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NCI-H2030 M4rYZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV;JR|UxRTF2Lk[0OFgh|ryP Mn7EV2FPT0WU
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LNCaP-Clone-FGC Mlr0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4\wbGlEPTB;MUWuOFI1QCEQvF2= NEfibJZUSU6JRWK=
CP66-MEL MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnrLTWM2OD1zNT60Olg5KM7:TR?= Mk\UV2FPT0WU
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NCI-H1092 NITNcolIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1fUfmlEPTB;MU[uPFMyPiEQvF2= M1jxfnNCVkeHUh?=
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NCI-H2405 NHrNSXNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUDJR|UxRTF5LkG5O|Qh|ryP MkDPV2FPT0WU
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MOLT-16 NH3lfZhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1zTUGlEPTB;MUeuPFk{PCEQvF2= M3XmWnNCVkeHUh?=
RH-18 NFPaU|FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MY\JR|UxRTF6LkCwN|Uh|ryP MVnTRW5ITVJ?
D-283MED M3n6VWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2fzZ2lEPTB;MUiuNFA5QCEQvF2= MXzTRW5ITVJ?
RCC10RGB NXuxeIRZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYjJR|UxRTF6LkC1OVkh|ryP NFry[|lUSU6JRWK=
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SCH NH\zTotIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MknKTWM2OD1zOD62OFU3KM7:TR?= MXnTRW5ITVJ?
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Saos-2 MoPiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF7HeXJKSzVyPUG5MlEyPjJizszN MUDTRW5ITVJ?
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NCI-H2122 MoHjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV7JR|UxRTN2LkO0O|Eh|ryP NWTaO2M{W0GQR1XS
ABC-1 MmrSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MULJR|UxRTN2LkO2PVMh|ryP MX3TRW5ITVJ?
NCI-H1355 Mor2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV;2eZJjUUN3ME2zOE4{QDh7IN88US=> M4fpVXNCVkeHUh?=
NOMO-1 MnPnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF3VTXNKSzVyPUO0MlU4ODNizszN NHHUfG5USU6JRWK=
MZ7-mel NI[4eHlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHnuTFVKSzVyPUO0MlY1QDdizszN NVnPS4V2W0GQR1XS
NCI-H810 NFrPcVhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX[wfFVtUUN3ME2zOE44Ojd6IN88US=> Ml65V2FPT0WU
D-392MG NFSxbZhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVfJR|UxRTN3LkC1OlUh|ryP NWjISXRQW0GQR1XS
LB771-HNC NUTTUo9[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1vEZ2lEPTB;M{WuNVY2QCEQvF2= M3i0SHNCVkeHUh?=
COLO-679 NIL3ZVlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVjJR|UxRTN3LkO4PEDPxE1? NH;tSpJUSU6JRWK=
SK-PN-DW NUPHZXl[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV7Hc4ZtUUN3ME2zOU41OzZ7IN88US=> NXLZOVRmW0GQR1XS
VA-ES-BJ NUHlOnRJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXLiTYpsUUN3ME2zOU41PjR7IN88US=> MW\TRW5ITVJ?
KNS-42 NGCyN45Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWXJR|UxRTN3LkW4NlYh|ryP MlTRV2FPT0WU
RVH-421 NHi5RmRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYDsOHVxUUN3ME2zOU44PjR3IN88US=> NHHVNnVUSU6JRWK=
P30-OHK NVXnTJRIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{D2TWlEPTB;M{WuPFc2OiEQvF2= M3:4OnNCVkeHUh?=
G-402 Mm[xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXnSNYZNUUN3ME2zOU46QDZ5IN88US=> MXfTRW5ITVJ?
S-117 NHPDS25Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkTTTWM2OD1|Nj6zNFA5KM7:TR?= MmG3V2FPT0WU
RXF393 MnG1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGKxOJBKSzVyPUO2MlM4QDVizszN M1raT3NCVkeHUh?=
LCLC-103H NYqyc|VzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1Gy[2lEPTB;M{[uO|gxOyEQvF2= NVm0XotuW0GQR1XS
U-118-MG M3S3dWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1fl[mlEPTB;M{[uPFg1PyEQvF2= M{XpWHNCVkeHUh?=
8-MG-BA MoXLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHPOS2FKSzVyPUO2Mlk1QDNizszN M3;2dHNCVkeHUh?=
MFH-ino NFLuPIdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXvaTFFIUUN3ME2zO{4{OzdzIN88US=> NGXKfVRUSU6JRWK=
NCI-H23 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV\JR|UxRTN5LkSxPVYh|ryP NHLGU3pUSU6JRWK=
NCI-H446 NHS1[5VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlnJTWM2OD1|Nz60PFYzKM7:TR?= MXvTRW5ITVJ?
LU-99A MnG4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVfJR|UxRTN5LkW3N|Eh|ryP MlLyV2FPT0WU
KP-N-YN M2LV[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGDFc3RKSzVyPUO3MlYyQTVizszN MYXTRW5ITVJ?
SCC-9 MnHoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXfRfm5UUUN3ME2zO{44QDN6IN88US=> MV7TRW5ITVJ?
EFM-19 M2DsNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXLJR|UxRTN5Lke5Olch|ryP MlG2V2FPT0WU
A427 M4q1dGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGW1UVdKSzVyPUO3MlkyODdizszN MV3TRW5ITVJ?
LB831-BLC NY\u[3NWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1;HcWlEPTB;M{iuOlA{QSEQvF2= NX7ZTGRyW0GQR1XS
HOS NInnWXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4C5b2lEPTB;M{muOlI4OyEQvF2= M1e3RnNCVkeHUh?=
LAN-6 Mm\VS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWfhOW1SUUN3ME20NE4xOTF{IN88US=> MoTOV2FPT0WU
U251 M2DtR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGfkPIpKSzVyPUSwMlQxPTRizszN NF34SWFUSU6JRWK=
SNU-387 M4HWZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUn0W3dNUUN3ME20NE41PTV|IN88US=> MUfTRW5ITVJ?
DMS-79 MlTpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGHiT29KSzVyPUSwMlU5QDlizszN MWDTRW5ITVJ?
SK-LU-1 NFPubYhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4jac2lEPTB;NECuOlU3QCEQvF2= MmPqV2FPT0WU
HT NFzp[mRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFzufHFKSzVyPUSwMlY3OzlizszN MX3TRW5ITVJ?
KP-N-YS MoDpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NULsOJZqUUN3ME20NE43PzZ6IN88US=> M3HVcHNCVkeHUh?=
DMS-114 M1XQXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVy4UZJRUUN3ME20NE45Ozd2IN88US=> NHPGV5hUSU6JRWK=
HUTU-80 M{XZTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmnnTWM2OD12MT6wOVY6KM7:TR?= MlHlV2FPT0WU
ONS-76 MkHuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmrTTWM2OD12MT60O|A6KM7:TR?= MnXDV2FPT0WU
A673 M4j5TGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NILwOHpKSzVyPUSxMlUyPTdizszN MX\TRW5ITVJ?
HPAF-II MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MU\JR|UxRTRzLk[3NFkh|ryP NHW5PItUSU6JRWK=
UACC-257 NFL5[o9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MULJR|UxRTRzLkezOlkh|ryP MonoV2FPT0WU
EW-22 NHTqZWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlPOTWM2OD12MT64Olk4KM7:TR?= MnGwV2FPT0WU
NCI-H1755 M1HrSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mo[5TWM2OD12MT64O|I2KM7:TR?= MlLxV2FPT0WU
KYSE-150 NXHze2poT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoTMTWM2OD12Mj6xO|gzKM7:TR?= NXrRVmliW0GQR1XS
CGTH-W-1 NHjrUY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIHSOFdKSzVyPUSyMlQ6PTVizszN MUPTRW5ITVJ?
NB13 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmnwTWM2OD12Mj62NlE4KM7:TR?= MWjTRW5ITVJ?
SW872 NHjNfYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnzkTWM2OD12Mj62N|MyKM7:TR?= M2e4UHNCVkeHUh?=
KP-4 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkfETWM2OD12Mj64PFMzKM7:TR?= NGq4VGpUSU6JRWK=
HCC38 M3T6eGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnX2TWM2OD12Mj65OlE5KM7:TR?= NGrsTWFUSU6JRWK=
BB49-HNC NGS5cJpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV7sOm9oUUN3ME20N{4zOzV4IN88US=> NVe3dWJ1W0GQR1XS
CAL-62 MnLnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV73VnNmUUN3ME20N{41ODJ6IN88US=> MlLMV2FPT0WU
NCI-H2029 NHu0ZZhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NU\rXHdoUUN3ME20N{42PThzIN88US=> NYPrSo0{W0GQR1XS
M14 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH7WV5ZKSzVyPUS0MlE{OzJizszN NEHGS2dUSU6JRWK=
COLO-680N M2OwfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{XtUWlEPTB;NESuN|M6KM7:TR?= Ml:0V2FPT0WU
Hs-578-T MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVnaT|l{UUN3ME20OU4xOjl2IN88US=> M4PmbHNCVkeHUh?=
MSTO-211H NXL2ZoJLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEnZT4ZKSzVyPUS1Mlk1QDJizszN NGLNTo5USU6JRWK=
EW-1 MofXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGnqd2hKSzVyPUS1Mlk3QDlizszN MorWV2FPT0WU
SW948 NYDDUVQ{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEPNWYtKSzVyPUS2MlQ1PjJizszN Mo\aV2FPT0WU
NCI-H460 NFXXfHRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXfJR|UxRTR4LkWyN{DPxE1? MY\TRW5ITVJ?
CAS-1 M1XXUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{L3eGlEPTB;NE[uO|cxOSEQvF2= M4rxcXNCVkeHUh?=
TGBC11TKB M4\0NWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF\mcXJKSzVyPUS3MlE4PzdizszN M3HC[nNCVkeHUh?=
HD-MY-Z NEDjZmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFjHZnJKSzVyPUS4Mlc5PiEQvF2= NEPkXJFUSU6JRWK=
NCI-H226 MofKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF3OVnBKSzVyPUS5Mlc4QDVizszN NF\5PFJUSU6JRWK=
PA-1 MlnVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX3MOY1jUUN3ME20PU45OzN3IN88US=> M4rnT3NCVkeHUh?=
LB2518-MEL M1fzcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXXJR|UxRTR7Lki3NlMh|ryP NITONG1USU6JRWK=
A101D M4PRVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYfJR|UxRTR7Lkm0NlUh|ryP Mm\XV2FPT0WU
NCI-H2087 Ml30S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYfJR|UxRTR7Lkm2PFIh|ryP M17xOXNCVkeHUh?=

... Click to View More Cell Line Experimental Data

In vivo Daily oral administration of BIBW2992 at 20 mg/kg for 25 days results in dramatic tumor regression with a cumulative treated/control tumor volume ratio (T/C ratio) of 2%. Reduced phosphorylation of EGFR and AKT is confirmed by immunohistochemical staining of tissue sections. Therefore, like lapatinib and neratinib, BIBW2992 is a next generation tyrosine kinase inhibitor (TKI) that inhibits human epidermal growth factor receptor 2 (Her2) and epidermal growth factor receptor (EGFR) kinases irreversibly. BIBW2992 is not only active against EGFR mutations targeted by first generation TKIs like Erlotinib or Gefitinib, but also against those insensitive to these standard therapies. [1]

Protocol

Kinase Assay:

[1]

+ Expand

In vitro kinase activity assays:

The wild type tyrosine kinase domain of the human EGFR as well as that of the EGFR L858R/T790M double mutant are fused to Glutathione-S-transferase (GST), and extracted. Enzyme activity is then assayed in the presence of the inhibitor BIBW2992, serially diluted in 50% DMSO. A random polymer pEY (4:1) is used as substrate and biotinylated pEY (bio-pEY) is added as a tracer substrate. The kinase domain of HER2 is cloned using the baculovirus system and extracted similarly to that of EGFR kinase. Details of assays for EGFR, HER2, SRC, BIRK and VEGFR2 kinase activity are available in Supplementary information.
Cell Research:

[1]

+ Expand
  • Cell lines: NSCLC cells
  • Concentrations: 0-10 μM
  • Incubation Time: 1 hour
  • Method:

    1 × 104 NSCLC cells are transferred into each well of a 96-well plate and cultured overnight in serum-free media for the EGFR phosphorylation assay. After addition of BIBW2992 on the next day, the plates are incubated at 37 °C for 1 hour. EGF-stimulation is done using 100 ng/mL for 10 min at room temperature. Cells are washed with ice cold PBS, extracted with 120 μL HEPEX buffer per well and shaken at room temperature for 1 hour. In all 2 × 104 cells per well is used for the HER2 phosphorylation assay. Streptavidin precoated plates are coated with anti-EGFR-biotin at 1:100 dilution in blocking buffer and c-erb2/HER2 oncoprotein Ab-5(Clone N24)-Biotin. Cell extracts is then transferred to the antibody-coated wells and incubated at room temperature for 1 hour. Extinction is measured at 450 nm.


    (Only for Reference)
Animal Research:

[1]

+ Expand
  • Animal Models: Athymic NMRI-nu/nu female mice
  • Formulation: In 0.5% methocellulose-0.4% polysorbate-80 (Tween 80)
  • Dosages: 20 mg/kg
  • Administration: Oral administration
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 97 mg/mL (199.61 mM)
Ethanol 15 mg/mL (30.86 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order:
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
10mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 485.94
Formula

C24H25ClFN5O3

CAS No. 439081-18-2
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02795156 Recruiting Non-small Cell Lung Carcinoma|Urothelial Carcinoma|Gastrointestinal Carcinoma, Non-colon|Upper Aerodigestive Tract Carcinoma SCRI Development Innovations, LLC|Foundation Medicine|Boehringer Ingelheim|Bayer September 28, 2016 Phase 2
NCT03054038 Not yet recruiting Non-Small Cell Lung Carcinoma Vanderbilt-Ingram Cancer Center|National Cancer Institute (NCI) February 2017 Phase 1
NCT02979977 Not yet recruiting Squamous Cell Cancers of the Head and Neck Aarti Bhatia|National Comprehensive Cancer Network|Boehringer Ingelheim|Yale University December 2016 Phase 2
NCT02876081 Not yet recruiting SMALL CELL LUNG CARCINOMA Groupe Hospitalier Paris Saint Joseph December 2016 Phase 2
NCT02183883 Recruiting Non-small Cell Lung Cancer University College, London|Boehringer Ingelheim December 2016 Phase 2
NCT02423525 Recruiting Brain Cancer Santosh Kesari|Boehringer Ingelheim|John Wayne Cancer Institute October 2016 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    What’s the recommendation about reconstitution of the compound for in vivo animal study?

  • Answer:

    For in vivo experiment of afatinib, we recommend 0.5% methylcellulose/0.2% Tween 80 as vehicle.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID