Afatinib (BIBW2992)

Catalog No.S1011

Afatinib (BIBW2992) Chemical Structure

Molecular Weight(MW): 485.94

Afatinib (BIBW2992) irreversibly inhibits EGFR/HER2 including EGFR(wt), EGFR(L858R), EGFR(L858R/T790M) and HER2 with IC50 of 0.5 nM, 0.4 nM, 10 nM and 14 nM in cell-free assays, respectively.

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In DMSO USD 240 In stock
USD 120 In stock
USD 210 In stock
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Cited by 37 Publications

4 Customer Reviews

  • Cytoxicity of irreversible EGFR TKIS and ethacrynic acid on breast cells lines. A, Mean IC50 value of Afatinib.

    Oncotarget, 2016, 7(36):58038-58050. Afatinib (BIBW2992) purchased from Selleck.

    Inhibition of signaling pathway activation in lung tumor cell lines by kinase inhibitors. Lung tumor cells were cultured in 10% FBS until reaching ∼80% confluence and then the cells were starved in serum-free medium for overnight, followed by 4-hour treatment with the inhibitors. Cell lysates were then prepared and used for determination of the pathway activation signals by the CEER assay.

    Int J Proteomics 2011 2011, 215496. Afatinib (BIBW2992) purchased from Selleck.

  • Inhibition of anchorage-independent growth of lung tumor cell lines by selected inhibitors. Each selected cell line was treated with the indicated inhibitor at 0.1 μM and 1 μM concentrations for two weeks and cell colony size formation was scored under the Nikon inverted-phase microscope.

    Int J Proteomics 2011 2011, 215496. Afatinib (BIBW2992) purchased from Selleck.

    After starved in serum-free medium for 24h, Breast cancer cells incubated with the indicated concentrations of BIBW2992 for 3h,followed by 15-minute stimolation of 100ng/ml EGF

    Dr. Zhang of Tianjin Medical University. Afatinib (BIBW2992) purchased from Selleck.

Purity & Quality Control

Choose Selective EGFR Inhibitors

Biological Activity

Description Afatinib (BIBW2992) irreversibly inhibits EGFR/HER2 including EGFR(wt), EGFR(L858R), EGFR(L858R/T790M) and HER2 with IC50 of 0.5 nM, 0.4 nM, 10 nM and 14 nM in cell-free assays, respectively.
Targets
EGFR (L858R) [1]
(Cell-free assay)
EGFR (wt) [1]
(Cell-free assay)
EGFR (L858R/T790M) [1]
(Cell-free assay)
HER2 [1]
(Cell-free assay)
0.4 nM 0.5 nM 10 nM 14 nM
In vitro

BIBW2992 shows potent activity against both wild-type and mutant forms of EGFR and HER2. It is similar to Gefitinib in potency against L858R EGFR, but about 100-fold more active against the Gefitinib resistant L858R-T790M EGFR double mutant. BIBW2992 exhibits potent effects on both EGFR and HER2 phosphorylation in vivo. It compares favorably to reference compounds (such as Lapatinib et al.) in all cell types tested, such as human epidermoid carcinoma cell line A431 expressing wt EGFR, murine NIH-3T3 cells transfected with wt HER2, as well as breast cancer cell line BT-474 and gastric cancer cell line NCI-N87, which express endogenous HER2. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HDLM-2 NY\jW3dZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mo\rTWM2OD1yLkCwOVczKM7:TR?= NX3EN|F5W0GQR1XS
KASUMI-1 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWfJR|UxRTBwMEC5NVEh|ryP NH\DUoZUSU6JRWK=
HCC2218 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWnJR|UxRTBwMEC5PFch|ryP NEDZb4lUSU6JRWK=
KARPAS-45 M2DGdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{PWUGlEPTB;MD6wNVY4OSEQvF2= M2TWZ3NCVkeHUh?=
HSC-4 M2jHTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWXsXGREUUN3ME2wMlAyPzR6IN88US=> NGPnN|lUSU6JRWK=
CHL-1 M4rsR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWLuTGhuUUN3ME2wMlAyQDN7IN88US=> NHLSPJFUSU6JRWK=
KY821 M125cGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn3JTWM2OD1yLkCyNFYh|ryP NEnZT2ZUSU6JRWK=
NCI-N87 M1PPZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4C0bmlEPTB;MD6wNlc4OyEQvF2= NIPFNpVUSU6JRWK=
DOK MoThS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWTJR|UxRTBwMEOxPVQh|ryP NVX4bY5rW0GQR1XS
DSH1 MnrXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1zxemlEPTB;MD6wN|MyPCEQvF2= NFnzSIJUSU6JRWK=
MDA-MB-175-VII MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2Xuc2lEPTB;MD6wN|QyPiEQvF2= MmPtV2FPT0WU
NCI-H2170 M1fQUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3T1eGlEPTB;MD6wN|czQCEQvF2= Ml\xV2FPT0WU
BT-474 M3HHWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHTW[IRKSzVyPUCuNFQ6QDZizszN Mmq2V2FPT0WU
EFO-27 NHrkV4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXfJR|UxRTBwMEWxPFIh|ryP M1TMUHNCVkeHUh?=
NCI-H720 Mo[3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M12zXWlEPTB;MD6wOVMzPSEQvF2= MkjqV2FPT0WU
PC-14 Ml3SS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1nDOmlEPTB;MD6wOVY3QCEQvF2= NIS3OJZUSU6JRWK=
EC-GI-10 M1LBdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUXJR|UxRTBwMEW5NVIh|ryP MornV2FPT0WU
KYSE-450 M4LGb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mln1TWM2OD1yLkC1PVc5KM7:TR?= NULKfGZ6W0GQR1XS
BPH-1 M1juRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnTzTWM2OD1yLkC1PVg5KM7:TR?= MXvTRW5ITVJ?
NCI-H292 NWf5c2x4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXf3V24zUUN3ME2wMlA3ODlizszN Mk\tV2FPT0WU
H9 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2Lab2lEPTB;MD6wO|E{OiEQvF2= NIrrZW5USU6JRWK=
NCI-H526 NYjjUGF[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGnWZWVKSzVyPUCuNFc5OjNizszN M{ftNnNCVkeHUh?=
NCI-H1623 MoPiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4f5[GlEPTB;MD6wPFAzPSEQvF2= NH7uSpVUSU6JRWK=
KYSE-140 NYLUSHEyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIT0O5ZKSzVyPUCuNFg{OyEQvF2= MmfFV2FPT0WU
Ca9-22 NYWyTotKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYjp[XVPUUN3ME2wMlA5OzV|IN88US=> NInZRVdUSU6JRWK=
AU565 MoH1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYLJR|UxRTBwMEi1NFQh|ryP M37kSnNCVkeHUh?=
ZR-75-30 NWTIU|Z3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWrJR|UxRTBwMEm1OFUh|ryP NF7ablBUSU6JRWK=
NCI-H1770 M{nnTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWrJR|UxRTBwMUCyPEDPxE1? MWPTRW5ITVJ?
LB2241-RCC NXjmS2JFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUCxUYk6UUN3ME2wMlExQDh2IN88US=> NWHpRVBYW0GQR1XS
CAL-39 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml;qTWM2OD1yLkGxO|kzKM7:TR?= MkTLV2FPT0WU
UACC-893 NWPz[nBnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{PJ[2lEPTB;MD6xNVg5PSEQvF2= MUnTRW5ITVJ?
LU-65 NInjNXZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXP3OWV4UUN3ME2wMlEzPjN|IN88US=> NFixNZdUSU6JRWK=
HN NY\DXIxnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYn0SWtDUUN3ME2wMlE{ODd5IN88US=> Mlz6V2FPT0WU
TI-73 NX;UcllXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXLyTHg4UUN3ME2wMlE1PzF3IN88US=> NVvO[I55W0GQR1XS
KU812 NUTpTYpQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{PD[2lEPTB;MD6xOVQ2PiEQvF2= MmLSV2FPT0WU
SW13 M3zNOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2nVTWlEPTB;MD6xOlIyPiEQvF2= Mom2V2FPT0WU
BB30-HNC NUPrZ4lvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV;JR|UxRTBwMU[1O|Yh|ryP MYPTRW5ITVJ?
HSC-2 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3HiTmlEPTB;MD6xOlg{PyEQvF2= MXvTRW5ITVJ?
BV-173 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{XydmlEPTB;MD6xO|U6PiEQvF2= M1m3PHNCVkeHUh?=
CAL-27 MmXwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYrvTZRGUUN3ME2wMlE4PjN4IN88US=> NGH4SI1USU6JRWK=
SBC-1 NF33RpFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mlj4TWM2OD1yLkG4PVU6KM7:TR?= NIHV[WJUSU6JRWK=
CAL-33 NWP1SVFkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnzPTWM2OD1yLkKwNVg1KM7:TR?= Mnn4V2FPT0WU
NCI-H209 NX\iSnJ7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH3WVWNKSzVyPUCuNlA1PiEQvF2= NGToNJlUSU6JRWK=
COR-L105 M{nXT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkT1TWM2OD1yLkKxNFg1KM7:TR?= MmLkV2FPT0WU
HSC-3 NGfQbZVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkGyTWM2OD1yLkKxNVk5KM7:TR?= MofpV2FPT0WU
NCI-H69 NY\yWpJCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYXJWHZLUUN3ME2wMlIyPDVzIN88US=> MnjpV2FPT0WU
MOLT-13 M3LMfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEexbGFKSzVyPUCuNlM6PzVizszN NW\IZm45W0GQR1XS
ML-2 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGricXdKSzVyPUCuNlQ5ODJizszN NHK4cWlUSU6JRWK=
IGROV-1 M1TURWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYLuOGtoUUN3ME2wMlI2QTV{IN88US=> M1TKOnNCVkeHUh?=
TK10 M1LJeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnvwTWM2OD1yLkK2O|M5KM7:TR?= M4PHb3NCVkeHUh?=
EoL-1-cell M2nScWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYHJR|UxRTBwMk[4PVIh|ryP NEfuNIlUSU6JRWK=
HO-1-N-1 M{PHWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkXsTWM2OD1yLkK3PFU{KM7:TR?= M4LV[XNCVkeHUh?=
BHY NUTmeJRpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mmm3TWM2OD1yLkK4OlU2KM7:TR?= Mn;MV2FPT0WU
LU-139 M3;uWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIPhcFZKSzVyPUCuN|A2OSEQvF2= NFf0fHhUSU6JRWK=
23132-87 NY\ER|FrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVPuTFNSUUN3ME2wMlMyQTJ|IN88US=> MX7TRW5ITVJ?
ACHN M4niUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX;JcoNNUUN3ME2wMlMyQTl2IN88US=> NFLheGZUSU6JRWK=
DoTc2-4510 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYLmSoszUUN3ME2wMlMzOjF7IN88US=> NGLNcYFUSU6JRWK=
BB65-RCC NXTkVoxiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYflOnJtUUN3ME2wMlM1PTl|IN88US=> M3vnNHNCVkeHUh?=
HLE NHvuUIJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIj4[pRKSzVyPUCuN|UxPDZizszN NFvYTZdUSU6JRWK=
HCC1419 M4XaWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFPp[5FKSzVyPUCuN|Y1ODhizszN NIXGT|lUSU6JRWK=
A388 M136cmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWrDeG9UUUN3ME2wMlM3QDV5IN88US=> MUDTRW5ITVJ?
NUGC-3 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlnKTWM2OD1yLkO4N|c{KM7:TR?= MlTaV2FPT0WU
KYSE-180 NHnq[2RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2HPU2lEPTB;MD6zPVA5QSEQvF2= NUL3[I5iW0GQR1XS
TE-6 NI\2Oo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnK0TWM2OD1yLkSwNlQ3KM7:TR?= M2Lo[XNCVkeHUh?=
A498 M3\tO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYHJR|UxRTBwNEC2O|Qh|ryP MnXLV2FPT0WU
NCI-H1648 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHT5WoZKSzVyPUCuOFQyQDJizszN M4rSdnNCVkeHUh?=
DU-145 NWLM[WZrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXzJR|UxRTBwNES4N|Mh|ryP MknLV2FPT0WU
CW-2 M3;xV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVHjSY14UUN3ME2wMlQ3QTF2IN88US=> MlnoV2FPT0WU
A431 NFnvOZNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHHHRlhKSzVyPUCuOFgzOzNizszN Mny3V2FPT0WU
SAS NYTzNohVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUm3ZXVWUUN3ME2wMlQ5Ozl6IN88US=> NHjXc25USU6JRWK=
NCI-H2126 NXvqd|JJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1;oc2lEPTB;MD61NVkzPiEQvF2= NV\i[oR2W0GQR1XS
NCI-H358 NUO1e5FFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHL5O|FKSzVyPUCuOVMzOSEQvF2= NG\ScGhUSU6JRWK=
FADU MmmxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVrneHJkUUN3ME2wMlU1PDB2IN88US=> Mn61V2FPT0WU
ACN M1fucmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{\vW2lEPTB;MD61OVA6PCEQvF2= MXjTRW5ITVJ?
HCC70 NYG4fYlXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYLJR|UxRTBwNUe2OlEh|ryP MUfTRW5ITVJ?
NCI-H1838 NX;jTINuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2f1VGlEPTB;MD62NFI6QSEQvF2= NVzIdnRlW0GQR1XS
CAL-54 NX3NU4hWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUDxWmVpUUN3ME2wMlYyPjJzIN88US=> M2TWUXNCVkeHUh?=
NCI-H1975 NGXMcYhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3;NRWlEPTB;MD62NVczQCEQvF2= NXHXNW9KW0GQR1XS
COR-L88 NIDrRm9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MljsTWM2OD1yLk[0NVE{KM7:TR?= M1PXPHNCVkeHUh?=
CTV-1 Ml70S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIriOY9KSzVyPUCuOlQ{QTJizszN MVnTRW5ITVJ?
EW-18 NVjMT3VtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF:3XopKSzVyPUCuOlg3QThizszN NYPQc4JsW0GQR1XS
HCC1806 NXn2NFF7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M17EXWlEPTB;MD62PVY3PiEQvF2= MVzTRW5ITVJ?
HuP-T4 Mnu1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1ryU2lEPTB;MD62PVc3KM7:TR?= M3H3PHNCVkeHUh?=
MDA-MB-361 MkXzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYrTNY9GUUN3ME2wMlczPjZ{IN88US=> NVS3bnlmW0GQR1XS
NCI-H1573 MmXuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{H4OmlEPTB;MD63N|Y{KM7:TR?= MnWxV2FPT0WU
KU-19-19 NVvVfWxIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkPFTWM2OD1yLke3OFY1KM7:TR?= NFHXR4ZUSU6JRWK=
COLO-678 M4HrSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkHQTWM2OD1yLkiwPFM1KM7:TR?= NVmybWZpW0GQR1XS
C-33-A NH2ySJNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mnq1TWM2OD1yLkixNlk4KM7:TR?= M4\D[XNCVkeHUh?=
Calu-3 NVvRTGduT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYXJR|UxRTBwOEG0N|Qh|ryP MXnTRW5ITVJ?
AGS MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2XDVGlEPTB;MD64N|Y1QCEQvF2= M3TBbXNCVkeHUh?=
SCC-25 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2nIOmlEPTB;MD64OFE4OSEQvF2= M4nmNnNCVkeHUh?=
JVM-2 Mnn1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYjZZnZRUUN3ME2wMlg5Ojl7IN88US=> NIPkVlBUSU6JRWK=
HCE-T MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIX1botKSzVyPUCuPFg1OjhizszN M2DpNHNCVkeHUh?=
ES4 NUThOYtjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml3FTWM2OD1yLkm3PFM3KM7:TR?= NFXw[pZUSU6JRWK=
EKVX M2PsW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUfJR|UxRTFwMEGwOlQh|ryP NWLEWGV{W0GQR1XS
D-542MG MoTXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1rMTmlEPTB;MT6wNVA3PCEQvF2= MoHDV2FPT0WU
SW1116 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUf3eotIUUN3ME2xMlAyOjh5IN88US=> NGT1U|hUSU6JRWK=
5637 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVfJR|UxRTFwMEG1NUDPxE1? NEjld2NUSU6JRWK=
HT-1376 M3zxW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlrVTWM2OD1zLkCxOVE{KM7:TR?= M2XN[HNCVkeHUh?=
NB69 M2fXb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkPYTWM2OD1zLkCyPVQ2KM7:TR?= NHrPNm9USU6JRWK=
YKG-1 NHW1NY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIG4dXJKSzVyPUGuNFU1OTlizszN NVvaZolSW0GQR1XS
EW-3 MmfUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIHFfYZKSzVyPUGuNFc5QDNizszN M{LnSXNCVkeHUh?=
GCIY MmXRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUjJR|UxRTFwMEixNFQh|ryP NEDiRoFUSU6JRWK=
SK-MES-1 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1\YeGlEPTB;MT6xNVQ5PyEQvF2= MVTTRW5ITVJ?
PANC-08-13 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGPxPW9KSzVyPUGuNVY1OTZizszN NXPodnhyW0GQR1XS
SK-NEP-1 NWjqTXFmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUmy[VNoUUN3ME2xMlE4PTF|IN88US=> M4raV3NCVkeHUh?=
SW954 M{TyTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWrW[oxSUUN3ME2xMlIxOjF2IN88US=> M37ZSnNCVkeHUh?=
NCI-SNU-5 M4L5NWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnHlTWM2OD1zLkKzPUDPxE1? MYPTRW5ITVJ?
SW48 NUXF[lZqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoSyTWM2OD1zLkK1OFMzKM7:TR?= NVjMbVBPW0GQR1XS
HT-3 NF7xT4VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnSyTWM2OD1zLkK4O|U{KM7:TR?= MkTzV2FPT0WU
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JVM-3 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NE\TSm9KSzVyPUeuPFk2QTlizszN MWfTRW5ITVJ?
OAW-28 NV\zdldET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYTJR|UxRTdwOUS1O|gh|ryP M3PI[nNCVkeHUh?=
KURAMOCHI NHfWfpJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnLZTWM2OD15Lkm0PFEyKM7:TR?= NUTrN2w6W0GQR1XS
BE-13 NWPDN3oyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnzRTWM2OD15Lkm4NVEzKM7:TR?= MXTTRW5ITVJ?
VMRC-RCZ NFexZ5pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGrleHFKSzVyPUiuNFE3QTdizszN Ml74V2FPT0WU
NCI-H727 MlHzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1;wR2lEPTB;OD6xNFQzKM7:TR?= MlyxV2FPT0WU
SW1710 M3vVV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4rYcWlEPTB;OD6xPFUyPSEQvF2= MVPTRW5ITVJ?
D-502MG MnPTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1z1TmlEPTB;OD6yPVI5OiEQvF2= Mo\XV2FPT0WU
NCI-H1993 M2fjW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVvJR|UxRThwM{O2N|Eh|ryP MWPTRW5ITVJ?
SCC-4 NETTS4NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVi3SJplUUN3ME24MlQ1ODV7IN88US=> MW\TRW5ITVJ?
HAL-01 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4nPOGlEPTB;OD61NVkyKM7:TR?= M1\5S3NCVkeHUh?=
GT3TKB NV3GVnJVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml3GTWM2OD16Lk[3PVY1KM7:TR?= M{jvV3NCVkeHUh?=
SK-OV-3 MnvlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1zIdGlEPTB;OD63NVMzQSEQvF2= NF7kTIdUSU6JRWK=
COLO-829 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NU[0VWYxUUN3ME24MlgxQTd4IN88US=> NXXI[XNtW0GQR1XS
OCI-AML2 NYjEXnVOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkezTWM2OD16Lkm1OFgyKM7:TR?= NGPxW4tUSU6JRWK=
OS-RC-2 NFvRXphIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1TLfWlEPTB;OT6wNlE6KM7:TR?= NGLMZZFUSU6JRWK=
BFTC-909 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXHJR|UxRTlwME[3PVkh|ryP Mn\0V2FPT0WU
EW-11 NHrn[JNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4DOS2lEPTB;OT6xO|Y2OyEQvF2= MlPXV2FPT0WU
HCE-4 M4rOVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUnJR|UxRTlwMkG1OVgh|ryP NF23UGtUSU6JRWK=
BALL-1 NG[0eHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4fX[WlEPTB;OT6yNVkxPyEQvF2= MnrrV2FPT0WU
SF539 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M17S[GlEPTB;OT6yN|U3OiEQvF2= NHPiRZdUSU6JRWK=
CAMA-1 NH;qc|FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWnrTI9{UUN3ME25MlI5OTJ6IN88US=> M2XV[XNCVkeHUh?=
C32 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2K4WGlEPTB;OT6zN|A3PCEQvF2= NEfsSlVUSU6JRWK=
NCI-H1703 NHjpU4RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NITVSpNKSzVyPUmuN|Q{PzVizszN NXXv[FNnW0GQR1XS
NCI-H1650 NYTVeZhST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWm2OZlTUUN3ME25MlYxQTR4IN88US=> M4KzPXNCVkeHUh?=
NCI-H1563 MlnUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHfIWWxKSzVyPUmuO|YxOzFizszN MXLTRW5ITVJ?
CAL-85-1 M3LZbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnvXTWM2OD17Lki3N|g{KM7:TR?= MYXTRW5ITVJ?
UM-UC-3 M3Hx[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3TNNWlEPTB;OT65OVA{KM7:TR?= NVvwSllNW0GQR1XS
BxPC-3 MlXTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX7YWmNNUUN3ME2xNE4xQTd{IN88US=> M1fRT3NCVkeHUh?=
MHH-PREB-1 NXnmOZFoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHmzdHZKSzVyPUGwMlE6QTlizszN MkHKV2FPT0WU
DEL NVvJSlBWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MorYTWM2OD1zMD6zPVQ3KM7:TR?= MWLTRW5ITVJ?
HuCCT1 M4DPemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEmwfWFKSzVyPUGwMlYxOzNizszN MYfTRW5ITVJ?
OVCAR-4 NGXLXW1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlLCTWM2OD1zMD64PFM2KM7:TR?= MVLTRW5ITVJ?
CAL-72 MoP3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIi4VVJKSzVyPUGwMlk{QTFizszN MVPTRW5ITVJ?
HCC1954 M2PkXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2HxO2lEPTB;MUGuNFc2PCEQvF2= MV7TRW5ITVJ?
EFO-21 NHexcJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M37SZ2lEPTB;MUGuNVA1PSEQvF2= NFfCe5ZUSU6JRWK=
FTC-133 NXXWPId[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkLBTWM2OD1zMT6xOFA4KM7:TR?= MmHMV2FPT0WU
SK-MEL-24 M4fHPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4DBS2lEPTB;MUGuNlM3PiEQvF2= NV3LVot6W0GQR1XS
UACC-62 M3\xSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYL5e3BkUUN3ME2xNU4zOzZ6IN88US=> M3;TbnNCVkeHUh?=
NCI-H650 NIO4fFFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2PNbmlEPTB;MUGuNlQyQCEQvF2= NYfuRWNZW0GQR1XS
ALL-PO MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYnJR|UxRTFzLkK1NVIh|ryP MlTzV2FPT0WU
T84 NFPYNVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGrGZYJKSzVyPUGxMlM6ODdizszN NYXuUZVQW0GQR1XS
IST-MEL1 Mnq4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVzBVFBUUUN3ME2xNU41PTV7IN88US=> NVjUVpNFW0GQR1XS
SW626 M2fBOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWTJR|UxRTFzLkW3OVUh|ryP M3v0[3NCVkeHUh?=
RPMI-2650 MmrIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXTJR|UxRTFzLkW4PFUh|ryP MonvV2FPT0WU
MC-IXC NEnUbIhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4r4fGlEPTB;MUGuOlczQCEQvF2= NW\HdYI3W0GQR1XS
HOP-62 M1q3Smdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVrJR|UxRTFzLk[3OlQh|ryP NFvzWmpUSU6JRWK=
NOS-1 NVrwV3hpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmLGTWM2OD1zMT63NVA6KM7:TR?= M2jUbnNCVkeHUh?=
SW1573 NVLNdIdkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHPvR5JKSzVyPUGxMlczQTVizszN MYXTRW5ITVJ?
WM-115 NFPiPWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYDHcZdqUUN3ME2xNU44PTh6IN88US=> M2GwdXNCVkeHUh?=
HuO9 NEXCfm5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmfHTWM2OD1zMT64O|c1KM7:TR?= NFKzWopUSU6JRWK=
YAPC NGDGSHFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGLVRZZKSzVyPUGxMlkzPTlizszN MnzIV2FPT0WU
MDA-MB-453 NXHRPY4yT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYTJR|UxRTFzLkm1NVUh|ryP MUPTRW5ITVJ?
NCI-H596 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{nDUGlEPTB;MUKuNFM3QSEQvF2= MmXBV2FPT0WU
A172 Mn\QS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIjqOJVKSzVyPUGyMlA4QThizszN MXjTRW5ITVJ?
RS4-11 NIftUIVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2TsTGlEPTB;MUKuNVczPSEQvF2= NUjzb2Z7W0GQR1XS
HCC1937 M3PJe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYHUTmNKUUN3ME2xNk44PTZ2IN88US=> NXTzbVh3W0GQR1XS
SW837 MmjVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4mwbGlEPTB;MUKuPVAzQCEQvF2= MX7TRW5ITVJ?
NMC-G1 NVHLXod3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1rlV2lEPTB;MUKuPVI{PCEQvF2= MYXTRW5ITVJ?
PFSK-1 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml7nTWM2OD1zMz6wOkDPxE1? MnztV2FPT0WU
DBTRG-05MG NXvab2RwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{HlS2lEPTB;MUOuNFY{OiEQvF2= NV[4[lkxW0GQR1XS
SK-UT-1 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1XQTGlEPTB;MUOuNVA5OyEQvF2= NIfjbINUSU6JRWK=
OVCAR-5 Moi4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXPObJBKUUN3ME2xN{4yPTl|IN88US=> NHvtVpRUSU6JRWK=
8505C MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUjJR|UxRTF|LkKwOlch|ryP NEnWNmJUSU6JRWK=
SK-MEL-1 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1PLWmlEPTB;MUOuO|E{PSEQvF2= M1[zUXNCVkeHUh?=
LK-2 NWf4RpVLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NG\sR4lKSzVyPUGzMlc3OzlizszN NUG0c4NRW0GQR1XS
OE33 NU[3OHl7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUPJR|UxRTF|Lke4OkDPxE1? MmKzV2FPT0WU
Ramos-2G6-4C10 NHK4SXNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXfVUppwUUN3ME2xOE4xOjB{IN88US=> NEHpN3JUSU6JRWK=
EW-13 NIjuNoFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NE\SNJdKSzVyPUG0MlA{PTJizszN NHPoe4xUSU6JRWK=
NCI-H1793 MmW5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmDoTWM2OD1zND6yNFg4KM7:TR?= NY\0XmsxW0GQR1XS
BHT-101 MmPVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXWzUJRXUUN3ME2xOE4zPDFizszN Mn\SV2FPT0WU
RKO MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MorJTWM2OD1zND61NFc2KM7:TR?= MmfzV2FPT0WU
NEC8 NXGyOGpzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1LWOWlEPTB;MUSuOVQ4OiEQvF2= MXrTRW5ITVJ?
PANC-10-05 NWLXVW5wT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHzScXRKSzVyPUG0MlYzPDdizszN Ml7hV2FPT0WU
NCI-H2030 MoO0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{HMNGlEPTB;MUSuOlQ1QCEQvF2= NUfpOHNqW0GQR1XS
C2BBe1 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MULJR|UxRTF2Lke2OEDPxE1? M1rMSHNCVkeHUh?=
EM-2 Ml;DS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4H0bWlEPTB;MUSuPFU4PiEQvF2= Ml2xV2FPT0WU
HCC1395 NE[2UWFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVXFW3B7UUN3ME2xOE46OzN6IN88US=> Mn;RV2FPT0WU
A2058 NXzpOJFGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF7aeXFKSzVyPUG1MlA1ODlizszN NWXUV5lZW0GQR1XS
SW1463 M3jxO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUXJR|UxRTF3LkC0PFUh|ryP Ml[4V2FPT0WU
J82 MknGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFfFcXlKSzVyPUG1MlA2PyEQvF2= MV;TRW5ITVJ?
CAL-120 M4HZZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUPBTlhIUUN3ME2xOU4yPDR5IN88US=> M4\YNXNCVkeHUh?=
COLO-824 M{XKN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWLJR|UxRTF3LkG1O|kh|ryP NYO4cnN[W0GQR1XS
LNCaP-Clone-FGC MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3T5NWlEPTB;MUWuOFI1QCEQvF2= MnL5V2FPT0WU
CP66-MEL MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHLUXXhKSzVyPUG1MlQ3QDhizszN NW\2U3pwW0GQR1XS
OC-314 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVPjU4VpUUN3ME2xOU41PzF2IN88US=> M3z4VHNCVkeHUh?=
OVCAR-8 NEfwPHJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MY\JR|UxRTF3Lki4OlEh|ryP M1LkfHNCVkeHUh?=
MFE-280 MoLzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2PIZ2lEPTB;MU[uNFQ5QCEQvF2= Mn\VV2FPT0WU
MHH-NB-11 MoP5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1W4NmlEPTB;MU[uNFg{QSEQvF2= Mkf5V2FPT0WU
SW1990 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mln3TWM2OD1zNj6xO|U2KM7:TR?= NInJNFJUSU6JRWK=
U-266 MnPTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYDJR|UxRTF4LkKyPVkh|ryP MlHIV2FPT0WU
MFM-223 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHvrfZFKSzVyPUG2MlM6PjVizszN MXHTRW5ITVJ?
L-363 MkLpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV7BeYxLUUN3ME2xOk41PTd5IN88US=> NYLZdmx{W0GQR1XS
MCF7 Mk[1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIW4cIRKSzVyPUG2MlQ4OjNizszN NFzIToJUSU6JRWK=
SK-MEL-3 NUTmUYZsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnfKTWM2OD1zNj60O|g4KM7:TR?= MmLsV2FPT0WU
HH NUezdmhDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlPXTWM2OD1zNj61NVYzKM7:TR?= NXq4SXBqW0GQR1XS
no-10 NGfpeHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXfJR|UxRTF4Lk[zOFMh|ryP MXLTRW5ITVJ?
HuP-T3 NEfEUYlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYrJR|UxRTF4Lk[1O|Yh|ryP M{XHSnNCVkeHUh?=
QIMR-WIL MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml7NTWM2OD1zNj63NVA2KM7:TR?= NUK4V|hIW0GQR1XS
NCI-H1092 MnfGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFT0SYVKSzVyPUG2Mlg{OTZizszN NYK4dZRtW0GQR1XS
MZ2-MEL NIfFVHpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHXIVoxKSzVyPUG2Mlg1ODNizszN MnrjV2FPT0WU
ES3 NF;iW2JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mo[zTWM2OD1zNj65NVM1KM7:TR?= NYrFT5VpW0GQR1XS
COLO-684 NIXwbHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnHvTWM2OD1zNj65OFc4KM7:TR?= NFLCXphUSU6JRWK=
NCI-H661 M3HqW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{TnT2lEPTB;MU[uPVY6PCEQvF2= MnfXV2FPT0WU
CP50-MEL-B MkCzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MknFTWM2OD1zNz6wN|Q{KM7:TR?= MmfsV2FPT0WU
NCI-H2405 M3y5U2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXi4V29GUUN3ME2xO{4yQTd2IN88US=> MXjTRW5ITVJ?
U-2-OS NVm0fYtGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWjJR|UxRTF5LkWzNFch|ryP NHXzVpFUSU6JRWK=
RERF-LC-MS MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYDJR|UxRTF5LkW1NFgh|ryP Mn\uV2FPT0WU
NCI-H2342 M2[1dGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEW1OpdKSzVyPUG3MlU2PyEQvF2= M1jJRnNCVkeHUh?=
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MOLT-16 MlPqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlXzTWM2OD1zNz64PVM1KM7:TR?= MVvTRW5ITVJ?
RH-18 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NITJcnlKSzVyPUG4MlAxOzVizszN NHTpXYFUSU6JRWK=
D-283MED M3\VS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIW1N4FKSzVyPUG4MlAxQDhizszN MVzTRW5ITVJ?
RCC10RGB M2DjXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHrTW3dKSzVyPUG4MlA2PTlizszN NFrHe3BUSU6JRWK=
NCI-H1437 NH:4N|ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHnHeFFKSzVyPUG4MlE3PDFizszN NH;MOnFUSU6JRWK=
SW982 M4PEZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYfJR|UxRTF6LkK2O|Eh|ryP NXzLOGNOW0GQR1XS
IST-MES1 NHPae3FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4DFZWlEPTB;MUiuOFQzQCEQvF2= NXLDSVFJW0GQR1XS
A704 NWjIXYlXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUjJR|UxRTF6LkS3OVMh|ryP M2Tre3NCVkeHUh?=
ESS-1 Mn\pS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWriSmN4UUN3ME2xPE42OjB3IN88US=> NVnoSWRVW0GQR1XS
SCH M3ey[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1ryNmlEPTB;MUiuOlQ2PiEQvF2= NHfhb4VUSU6JRWK=
COLO-320-HSR M4rERmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH\ZW3lKSzVyPUG4Mlc1PiEQvF2= M4HJd3NCVkeHUh?=
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KM-H2 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NILJU|NKSzVyPUO0MlE4PzlizszN M33ENXNCVkeHUh?=
NCI-H2122 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVzJR|UxRTN2LkO0O|Eh|ryP NFjFSmFUSU6JRWK=
ABC-1 MmPmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWfTb41FUUN3ME2zOE4{Pjl|IN88US=> NV;Vbm9xW0GQR1XS
NCI-H1355 NVnXfYRLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF\OWYZKSzVyPUO0MlM5QDlizszN Ml[2V2FPT0WU
NOMO-1 NH7qeHpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlX0TWM2OD1|ND61O|A{KM7:TR?= M3HqOHNCVkeHUh?=
MZ7-mel NHPEcVdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXfhSHhxUUN3ME2zOE43PDh5IN88US=> NGW1TodUSU6JRWK=
NCI-H810 MnvuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIrEc2xKSzVyPUO0MlczPzhizszN MULTRW5ITVJ?
D-392MG M1TZT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYrJR|UxRTN3LkC1OlUh|ryP NIHHUpZUSU6JRWK=
LB771-HNC MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NULsSZl{UUN3ME2zOU4yPjV6IN88US=> MonYV2FPT0WU
COLO-679 NFLHO|JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYPJR|UxRTN3LkO4PEDPxE1? NXXiWFVlW0GQR1XS
SK-PN-DW NFHUWYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnfPTWM2OD1|NT60N|Y6KM7:TR?= MWnTRW5ITVJ?
VA-ES-BJ MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmTUTWM2OD1|NT60OlQ6KM7:TR?= NHjPV45USU6JRWK=
KNS-42 MnjMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4XEdmlEPTB;M{WuOVgzPiEQvF2= MUjTRW5ITVJ?
RVH-421 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIfsc|ZKSzVyPUO1Mlc3PDVizszN MmGyV2FPT0WU
P30-OHK NVTVOoFRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnzQTWM2OD1|NT64O|UzKM7:TR?= NYjG[mRZW0GQR1XS
G-402 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUPJR|UxRTN3Lkm4Olch|ryP M1z1enNCVkeHUh?=
S-117 MmmzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH3POZFKSzVyPUO2MlMxODhizszN M3L0THNCVkeHUh?=
RXF393 MoC2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M372dWlEPTB;M{[uN|c5PSEQvF2= M3[yd3NCVkeHUh?=
LCLC-103H MlrnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4HXZ2lEPTB;M{[uO|gxOyEQvF2= M4\m[nNCVkeHUh?=
U-118-MG NVv3PZF{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkPDTWM2OD1|Nj64PFQ4KM7:TR?= MVvTRW5ITVJ?
8-MG-BA NYruNVdET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYLRPVB{UUN3ME2zOk46PDh|IN88US=> M1fq[3NCVkeHUh?=
MFH-ino NH\w[IVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV;SUVFoUUN3ME2zO{4{OzdzIN88US=> NIXV[4RUSU6JRWK=
NCI-H23 NW[zUXBDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoWwTWM2OD1|Nz60NVk3KM7:TR?= NV7RboM5W0GQR1XS
NCI-H446 NFLxVnNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFm1b2NKSzVyPUO3MlQ5PjJizszN MoD6V2FPT0WU
LU-99A M3H5Zmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYDJR|UxRTN5LkW3N|Eh|ryP NHTFeHJUSU6JRWK=
KP-N-YN MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH;ISHdKSzVyPUO3MlYyQTVizszN MYTTRW5ITVJ?
SCC-9 M172[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF\KSHJKSzVyPUO3Mlc5OzhizszN MYfTRW5ITVJ?
EFM-19 NX7IWHd5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{DFdWlEPTB;M{euO|k3PyEQvF2= MUTTRW5ITVJ?
A427 M1X6[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXnJR|UxRTN5LkmxNFch|ryP NE[1TpNUSU6JRWK=
LB831-BLC MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlG3TWM2OD1|OD62NFM6KM7:TR?= NHz5OIxUSU6JRWK=
HOS MmfLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnX1TWM2OD1|OT62Nlc{KM7:TR?= MmTrV2FPT0WU
LAN-6 NXTYdYpGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlLNTWM2OD12MD6wNVEzKM7:TR?= NYLPTGNDW0GQR1XS
U251 MmTOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoC3TWM2OD12MD60NFU1KM7:TR?= NWjYdmtUW0GQR1XS
SNU-387 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVvnVFN7UUN3ME20NE41PTV|IN88US=> NVrmeGV3W0GQR1XS
DMS-79 MmjlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH\uW2FKSzVyPUSwMlU5QDlizszN NFnQOm1USU6JRWK=
SK-LU-1 NHjhO2tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmnHTWM2OD12MD62OVY5KM7:TR?= M1HxV3NCVkeHUh?=
HT NEjBR21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH7OWJZKSzVyPUSwMlY3OzlizszN M2\sTXNCVkeHUh?=
KP-N-YS NYrYXpV4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEfJOVdKSzVyPUSwMlY4PjhizszN NGjYN4VUSU6JRWK=
DMS-114 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1u5WWlEPTB;NECuPFM4PCEQvF2= MmjRV2FPT0WU
HUTU-80 M4rhPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXzjfoU4UUN3ME20NU4xPTZ7IN88US=> NH7kfG9USU6JRWK=
ONS-76 M2S2RWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mnz3TWM2OD12MT60O|A6KM7:TR?= M362Z3NCVkeHUh?=
A673 MkLkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF61[nFKSzVyPUSxMlUyPTdizszN M4LhS3NCVkeHUh?=
HPAF-II MlnMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYLJR|UxRTRzLk[3NFkh|ryP NYTlTZl2W0GQR1XS
UACC-257 MnLtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{ThR2lEPTB;NEGuO|M3QSEQvF2= MV;TRW5ITVJ?
EW-22 NYjtc|dLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmXrTWM2OD12MT64Olk4KM7:TR?= NES4enRUSU6JRWK=
NCI-H1755 MoP3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYrJR|UxRTRzLki3NlUh|ryP MoTpV2FPT0WU
KYSE-150 MoXSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGLCO49KSzVyPUSyMlE4QDJizszN MVPTRW5ITVJ?
CGTH-W-1 M1;CTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnrZTWM2OD12Mj60PVU2KM7:TR?= M4nZVHNCVkeHUh?=
NB13 NXjH[ZNKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3jSW2lEPTB;NEKuOlIyPyEQvF2= NFfPT4tUSU6JRWK=
SW872 NITmNm5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml60TWM2OD12Mj62N|MyKM7:TR?= NHHwcldUSU6JRWK=
KP-4 MkjaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYfJR|UxRTR{Lki4N|Ih|ryP MnLYV2FPT0WU
HCC38 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnLUTWM2OD12Mj65OlE5KM7:TR?= NVnENXM{W0GQR1XS
BB49-HNC MkXES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEnrSIlKSzVyPUSzMlI{PTZizszN MUTTRW5ITVJ?
CAL-62 M4[5e2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnzpTWM2OD12Mz60NFI5KM7:TR?= M33tPHNCVkeHUh?=
NCI-H2029 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIL6W49KSzVyPUSzMlU2QDFizszN MXvTRW5ITVJ?
M14 NWPJWZZVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2nTcWlEPTB;NESuNVM{OiEQvF2= NFrrbohUSU6JRWK=
COLO-680N M3vmeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2S5PWlEPTB;NESuN|M6KM7:TR?= MWPTRW5ITVJ?
Hs-578-T MkHES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4fRS2lEPTB;NEWuNFI6PCEQvF2= NFqyUJZUSU6JRWK=
MSTO-211H NWDGbVF2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV3JR|UxRTR3Lkm0PFIh|ryP NVvqbVNZW0GQR1XS
EW-1 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX\POYpiUUN3ME20OU46Pjh7IN88US=> NUjBNppkW0GQR1XS
SW948 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUK3XJU6UUN3ME20Ok41PDZ{IN88US=> M{jlR3NCVkeHUh?=
NCI-H460 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV3ZTo9rUUN3ME20Ok42OjNizszN MlzIV2FPT0WU
CAS-1 NWDHfXlET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUfQdmhKUUN3ME20Ok44PzBzIN88US=> NUnx[GtuW0GQR1XS
TGBC11TKB M33WTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{TDNGlEPTB;NEeuNVc4PyEQvF2= NVHVblViW0GQR1XS
HD-MY-Z M33MNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1e2TmlEPTB;NEiuO|g3KM7:TR?= M{H0SnNCVkeHUh?=
NCI-H226 MkHHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXnJR|UxRTR7Lke3PFUh|ryP Mn7nV2FPT0WU
PA-1 NUK5bFVrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NU\HVGY1UUN3ME20PU45OzN3IN88US=> MW\TRW5ITVJ?
LB2518-MEL MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MU\JR|UxRTR7Lki3NlMh|ryP NH\LRYNUSU6JRWK=
A101D Ml23S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MU\JR|UxRTR7Lkm0NlUh|ryP NX3KOIJXW0GQR1XS
NCI-H2087 NEnpflJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2LQeWlEPTB;NEmuPVY5OiEQvF2= M2\zOHNCVkeHUh?=

... Click to View More Cell Line Experimental Data

In vivo Daily oral administration of BIBW2992 at 20 mg/kg for 25 days results in dramatic tumor regression with a cumulative treated/control tumor volume ratio (T/C ratio) of 2%. Reduced phosphorylation of EGFR and AKT is confirmed by immunohistochemical staining of tissue sections. Therefore, like lapatinib and neratinib, BIBW2992 is a next generation tyrosine kinase inhibitor (TKI) that inhibits human epidermal growth factor receptor 2 (Her2) and epidermal growth factor receptor (EGFR) kinases irreversibly. BIBW2992 is not only active against EGFR mutations targeted by first generation TKIs like Erlotinib or Gefitinib, but also against those insensitive to these standard therapies. [1]

Protocol

Kinase Assay:

[1]

+ Expand

In vitro kinase activity assays:

The wild type tyrosine kinase domain of the human EGFR as well as that of the EGFR L858R/T790M double mutant are fused to Glutathione-S-transferase (GST), and extracted. Enzyme activity is then assayed in the presence of the inhibitor BIBW2992, serially diluted in 50% DMSO. A random polymer pEY (4:1) is used as substrate and biotinylated pEY (bio-pEY) is added as a tracer substrate. The kinase domain of HER2 is cloned using the baculovirus system and extracted similarly to that of EGFR kinase. Details of assays for EGFR, HER2, SRC, BIRK and VEGFR2 kinase activity are available in Supplementary information.
Cell Research:

[1]

+ Expand
  • Cell lines: NSCLC cells
  • Concentrations: 0-10 μM
  • Incubation Time: 1 hour
  • Method:

    1 × 104 NSCLC cells are transferred into each well of a 96-well plate and cultured overnight in serum-free media for the EGFR phosphorylation assay. After addition of BIBW2992 on the next day, the plates are incubated at 37 °C for 1 hour. EGF-stimulation is done using 100 ng/mL for 10 min at room temperature. Cells are washed with ice cold PBS, extracted with 120 μL HEPEX buffer per well and shaken at room temperature for 1 hour. In all 2 × 104 cells per well is used for the HER2 phosphorylation assay. Streptavidin precoated plates are coated with anti-EGFR-biotin at 1:100 dilution in blocking buffer and c-erb2/HER2 oncoprotein Ab-5(Clone N24)-Biotin. Cell extracts is then transferred to the antibody-coated wells and incubated at room temperature for 1 hour. Extinction is measured at 450 nm.


    (Only for Reference)
Animal Research:

[1]

+ Expand
  • Animal Models: Athymic NMRI-nu/nu female mice
  • Formulation: In 0.5% methocellulose-0.4% polysorbate-80 (Tween 80)
  • Dosages: 20 mg/kg
  • Administration: Oral administration
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 97 mg/mL (199.61 mM)
Ethanol 15 mg/mL (30.86 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order:
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
10mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 485.94
Formula

C24H25ClFN5O3

CAS No. 439081-18-2
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

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Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02795156 Recruiting Non-small Cell Lung Carcinoma|Urothelial Carcinoma|Gastrointestinal Carcinoma, Non-colon|Upper Aerodigestive Tract Carcinoma SCRI Development Innovations, LLC|Foundation Medicine|Boehringer Ingelheim|Bayer September 28, 2016 Phase 2
NCT03054038 Not yet recruiting Non-Small Cell Lung Carcinoma Vanderbilt-Ingram Cancer Center|National Cancer Institute (NCI) February 2017 Phase 1
NCT02979977 Not yet recruiting Squamous Cell Cancers of the Head and Neck Aarti Bhatia|National Comprehensive Cancer Network|Boehringer Ingelheim|Yale University December 2016 Phase 2
NCT02876081 Not yet recruiting SMALL CELL LUNG CARCINOMA Groupe Hospitalier Paris Saint Joseph December 2016 Phase 2
NCT02183883 Recruiting Non-small Cell Lung Cancer University College, London|Boehringer Ingelheim December 2016 Phase 2
NCT02423525 Recruiting Brain Cancer Santosh Kesari|Boehringer Ingelheim|John Wayne Cancer Institute October 2016 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    What’s the recommendation about reconstitution of the compound for in vivo animal study?

  • Answer:

    For in vivo experiment of afatinib, we recommend 0.5% methylcellulose/0.2% Tween 80 as vehicle.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID