Afatinib (BIBW2992)

Catalog No.S1011

Afatinib (BIBW2992) Chemical Structure

Molecular Weight(MW): 485.94

Afatinib (BIBW2992) irreversibly inhibits EGFR/HER2 including EGFR(wt), EGFR(L858R), EGFR(L858R/T790M) and HER2 with IC50 of 0.5 nM, 0.4 nM, 10 nM and 14 nM in cell-free assays, respectively.

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In DMSO USD 240 In stock
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USD 210 In stock
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Cited by 37 Publications

4 Customer Reviews

  • Cytoxicity of irreversible EGFR TKIS and ethacrynic acid on breast cells lines. A, Mean IC50 value of Afatinib.

    Oncotarget, 2016, 7(36):58038-58050. Afatinib (BIBW2992) purchased from Selleck.

    Inhibition of signaling pathway activation in lung tumor cell lines by kinase inhibitors. Lung tumor cells were cultured in 10% FBS until reaching ∼80% confluence and then the cells were starved in serum-free medium for overnight, followed by 4-hour treatment with the inhibitors. Cell lysates were then prepared and used for determination of the pathway activation signals by the CEER assay.

    Int J Proteomics 2011 2011, 215496. Afatinib (BIBW2992) purchased from Selleck.

  • Inhibition of anchorage-independent growth of lung tumor cell lines by selected inhibitors. Each selected cell line was treated with the indicated inhibitor at 0.1 μM and 1 μM concentrations for two weeks and cell colony size formation was scored under the Nikon inverted-phase microscope.

    Int J Proteomics 2011 2011, 215496. Afatinib (BIBW2992) purchased from Selleck.

    After starved in serum-free medium for 24h, Breast cancer cells incubated with the indicated concentrations of BIBW2992 for 3h,followed by 15-minute stimolation of 100ng/ml EGF

    Dr. Zhang of Tianjin Medical University. Afatinib (BIBW2992) purchased from Selleck.

Purity & Quality Control

Choose Selective EGFR Inhibitors

Biological Activity

Description Afatinib (BIBW2992) irreversibly inhibits EGFR/HER2 including EGFR(wt), EGFR(L858R), EGFR(L858R/T790M) and HER2 with IC50 of 0.5 nM, 0.4 nM, 10 nM and 14 nM in cell-free assays, respectively.
Targets
EGFR (L858R) [1]
(Cell-free assay)
EGFR (wt) [1]
(Cell-free assay)
EGFR (L858R/T790M) [1]
(Cell-free assay)
HER2 [1]
(Cell-free assay)
0.4 nM 0.5 nM 10 nM 14 nM
In vitro

BIBW2992 shows potent activity against both wild-type and mutant forms of EGFR and HER2. It is similar to Gefitinib in potency against L858R EGFR, but about 100-fold more active against the Gefitinib resistant L858R-T790M EGFR double mutant. BIBW2992 exhibits potent effects on both EGFR and HER2 phosphorylation in vivo. It compares favorably to reference compounds (such as Lapatinib et al.) in all cell types tested, such as human epidermoid carcinoma cell line A431 expressing wt EGFR, murine NIH-3T3 cells transfected with wt HER2, as well as breast cancer cell line BT-474 and gastric cancer cell line NCI-N87, which express endogenous HER2. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HDLM-2 Ml;CS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXfye5RsUUN3ME2wMlAxPTd{IN88US=> NVXRW21YW0GQR1XS
KASUMI-1 NVHsc2RzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWfqdGN5UUN3ME2wMlAxQTFzIN88US=> M2\wVnNCVkeHUh?=
HCC2218 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4C3[GlEPTB;MD6wNFk5PyEQvF2= MX7TRW5ITVJ?
KARPAS-45 NVT3Z|RmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIrwcolKSzVyPUCuNFE3PzFizszN NFu4dW1USU6JRWK=
HSC-4 M3TEW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUDle4lOUUN3ME2wMlAyPzR6IN88US=> M{m2d3NCVkeHUh?=
CHL-1 M13O[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mk\tTWM2OD1yLkCxPFM6KM7:TR?= MlL0V2FPT0WU
KY821 NF7GToZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2Xm[WlEPTB;MD6wNlA3KM7:TR?= Moq4V2FPT0WU
NCI-N87 MoTmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX\JR|UxRTBwMEK3O|Mh|ryP NHHqXpNUSU6JRWK=
DOK M2TWSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEj5OYJKSzVyPUCuNFMyQTRizszN M4PaW3NCVkeHUh?=
DSH1 NVrwUoM2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1\NUWlEPTB;MD6wN|MyPCEQvF2= MYDTRW5ITVJ?
MDA-MB-175-VII NY\CNXJOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWXZW|BIUUN3ME2wMlA{PDF4IN88US=> MnjMV2FPT0WU
NCI-H2170 M{\zPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH\EZ3VKSzVyPUCuNFM4OjhizszN M1zwe3NCVkeHUh?=
BT-474 NHXrT5JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MonpTWM2OD1yLkC0PVg3KM7:TR?= NV3DdXRZW0GQR1XS
EFO-27 M2Xvemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXjJR|UxRTBwMEWxPFIh|ryP NUXkbIVUW0GQR1XS
NCI-H720 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXT1Z2lsUUN3ME2wMlA2OzJ3IN88US=> NVq2dW1vW0GQR1XS
PC-14 NIr1WGJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmW2TWM2OD1yLkC1OlY5KM7:TR?= M4Ww[3NCVkeHUh?=
EC-GI-10 NIrneG5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV\JR|UxRTBwMEW5NVIh|ryP NEfmNnFUSU6JRWK=
KYSE-450 NGPWeFBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3vaVWlEPTB;MD6wOVk4QCEQvF2= M1TTSHNCVkeHUh?=
BPH-1 MmOwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXXJR|UxRTBwMEW5PFgh|ryP MnXJV2FPT0WU
NCI-H292 M4ToW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWDJR|UxRTBwME[wPUDPxE1? MmXlV2FPT0WU
H9 MmG3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYLRemU{UUN3ME2wMlA4OTN{IN88US=> MV3TRW5ITVJ?
NCI-H526 Mm\zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3vPVGlEPTB;MD6wO|gzOyEQvF2= Mn3oV2FPT0WU
NCI-H1623 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXfJR|UxRTBwMEiwNlUh|ryP NFvjWpVUSU6JRWK=
KYSE-140 NX;CUm5WT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn76TWM2OD1yLkC4N|Mh|ryP Ml;DV2FPT0WU
Ca9-22 NWrZcJRzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIXxZpdKSzVyPUCuNFg{PTNizszN MonHV2FPT0WU
AU565 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWHJR|UxRTBwMEi1NFQh|ryP MmjrV2FPT0WU
ZR-75-30 NWHmVZlUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml3VTWM2OD1yLkC5OVQ2KM7:TR?= M2DIWXNCVkeHUh?=
NCI-H1770 MnPXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml3WTWM2OD1yLkGwNlgh|ryP NGrkW2JUSU6JRWK=
LB2241-RCC MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYfoem5MUUN3ME2wMlExQDh2IN88US=> NFv2S2dUSU6JRWK=
CAL-39 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2nFZ2lEPTB;MD6xNVc6OiEQvF2= NGXFWJhUSU6JRWK=
UACC-893 NXK2OlBET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWXicJN[UUN3ME2wMlEyQDh3IN88US=> NXjSc5BHW0GQR1XS
LU-65 NX\acJdVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEHwc25KSzVyPUCuNVI3OzNizszN MXPTRW5ITVJ?
HN MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn[xTWM2OD1yLkGzNFc4KM7:TR?= M3\DSXNCVkeHUh?=
TI-73 NGrrN4pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFT0eGFKSzVyPUCuNVQ4OTVizszN M2O2e3NCVkeHUh?=
KU812 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYDJR|UxRTBwMUW0OVYh|ryP NVPoSWx3W0GQR1XS
SW13 MlnJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlTSTWM2OD1yLkG2NlE3KM7:TR?= MWjTRW5ITVJ?
BB30-HNC M4mxSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml7KTWM2OD1yLkG2OVc3KM7:TR?= NFnpXI9USU6JRWK=
HSC-2 NHnCZ3RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NETzdnBKSzVyPUCuNVY5OzdizszN MlXYV2FPT0WU
BV-173 NUnYWIlrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn\6TWM2OD1yLkG3OVk3KM7:TR?= MUXTRW5ITVJ?
CAL-27 M4fIc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmXKTWM2OD1yLkG3OlM3KM7:TR?= MUTTRW5ITVJ?
SBC-1 NYm4VoVnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFL1bXRKSzVyPUCuNVg6PTlizszN NUDFUoNOW0GQR1XS
CAL-33 MlnZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoHsTWM2OD1yLkKwNVg1KM7:TR?= MorCV2FPT0WU
NCI-H209 M4\uUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGPJVGxKSzVyPUCuNlA1PiEQvF2= M3\nTnNCVkeHUh?=
COR-L105 NGGy[JlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFXrRnlKSzVyPUCuNlExQDRizszN Mn\sV2FPT0WU
HSC-3 NVHuTm84T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoLvTWM2OD1yLkKxNVk5KM7:TR?= NGOzdGJUSU6JRWK=
NCI-H69 NViyU2dMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXLMNWN4UUN3ME2wMlIyPDVzIN88US=> Mn:0V2FPT0WU
MOLT-13 NUPpWZNxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{nIcWlEPTB;MD6yN|k4PSEQvF2= NGGzR3NUSU6JRWK=
ML-2 NFTWSGdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkD0TWM2OD1yLkK0PFAzKM7:TR?= NIrqd|JUSU6JRWK=
IGROV-1 NXGyc5hxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF2zTGVKSzVyPUCuNlU6PTJizszN MXrTRW5ITVJ?
TK10 NFzJUWVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWnRUY4yUUN3ME2wMlI3PzN6IN88US=> NWnReGJYW0GQR1XS
EoL-1-cell NW[3SnJRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{DjbGlEPTB;MD6yOlg6OiEQvF2= NWPQe3ZtW0GQR1XS
HO-1-N-1 MkLJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGXs[5hKSzVyPUCuNlc5PTNizszN MojMV2FPT0WU
BHY NWH0VHg{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3\kfGlEPTB;MD6yPFY2PSEQvF2= M2LpVXNCVkeHUh?=
LU-139 NUTMeYVQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NY\zV2I4UUN3ME2wMlMxPTFizszN MVnTRW5ITVJ?
23132-87 M33VNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYTJR|UxRTBwM{G5NlMh|ryP NX33RZU3W0GQR1XS
ACHN NV;FXXN{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnHyTWM2OD1yLkOxPVk1KM7:TR?= MoixV2FPT0WU
DoTc2-4510 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1K4fGlEPTB;MD6zNlIyQSEQvF2= NH7C[HhUSU6JRWK=
BB65-RCC MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGnpNJdKSzVyPUCuN|Q2QTNizszN M4f4e3NCVkeHUh?=
HLE NEXL[FJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2rCeGlEPTB;MD6zOVA1PiEQvF2= M3H3T3NCVkeHUh?=
HCC1419 MoTJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4\zeWlEPTB;MD6zOlQxQCEQvF2= M3ezUHNCVkeHUh?=
A388 MnvCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1LBc2lEPTB;MD6zOlg2PyEQvF2= NYH1[XV5W0GQR1XS
NUGC-3 MmL5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXW4eosyUUN3ME2wMlM5Ozd|IN88US=> M4i2WnNCVkeHUh?=
KYSE-180 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXXJR|UxRTBwM{mwPFkh|ryP NECzcXdUSU6JRWK=
TE-6 MljES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEXqWJJKSzVyPUCuOFAzPDZizszN M1nucnNCVkeHUh?=
A498 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGjndpFKSzVyPUCuOFA3PzRizszN NYC4WnFJW0GQR1XS
NCI-H1648 NUj6Not[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1;3RmlEPTB;MD60OFE5OiEQvF2= Ml;QV2FPT0WU
DU-145 M4LPZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn;nTWM2OD1yLkS0PFM{KM7:TR?= NGjIWIlUSU6JRWK=
CW-2 MluyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2DIV2lEPTB;MD60OlkyPCEQvF2= NGTLT3lUSU6JRWK=
A431 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWXEUnM2UUN3ME2wMlQ5OjN|IN88US=> MVfTRW5ITVJ?
SAS NYe4[HoyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2fYemlEPTB;MD60PFM6QCEQvF2= MULTRW5ITVJ?
NCI-H2126 NVLxc5VFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFjuPWhKSzVyPUCuOVE6OjZizszN NVPuWXNIW0GQR1XS
NCI-H358 NXnVSFBoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2LkSGlEPTB;MD61N|IyKM7:TR?= M2LHSHNCVkeHUh?=
FADU NELoTnVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUPRPG17UUN3ME2wMlU1PDB2IN88US=> MXPTRW5ITVJ?
ACN M3PHXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M13VNGlEPTB;MD61OVA6PCEQvF2= M3TZSXNCVkeHUh?=
HCC70 NV7j[IhET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYjJR|UxRTBwNUe2OlEh|ryP MXfTRW5ITVJ?
NCI-H1838 MorQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmrBTWM2OD1yLk[wNlk6KM7:TR?= MV7TRW5ITVJ?
CAL-54 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlLzTWM2OD1yLk[xOlIyKM7:TR?= NIfXbW1USU6JRWK=
NCI-H1975 NFTmNGlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4f0RWlEPTB;MD62NVczQCEQvF2= NEWyXJFUSU6JRWK=
COR-L88 M1vRZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXXJR|UxRTBwNkSxNVMh|ryP M4iwRnNCVkeHUh?=
CTV-1 NHPt[JRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVPWUI5WUUN3ME2wMlY1Ozl{IN88US=> NYj6OG5YW0GQR1XS
EW-18 NX3U[HB7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIrySHVKSzVyPUCuOlg3QThizszN NWHxPIcyW0GQR1XS
HCC1806 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVXJR|UxRTBwNkm2OlYh|ryP MmfQV2FPT0WU
HuP-T4 NHzHVnJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1Lyb2lEPTB;MD62PVc3KM7:TR?= NVTsNFBYW0GQR1XS
MDA-MB-361 MmfyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mly0TWM2OD1yLkeyOlYzKM7:TR?= MlfyV2FPT0WU
NCI-H1573 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGTsPJdKSzVyPUCuO|M3OyEQvF2= NXHwSWw2W0GQR1XS
KU-19-19 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXnJV2VOUUN3ME2wMlc4PDZ2IN88US=> M1PsOXNCVkeHUh?=
COLO-678 NYXodHNpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mkn4TWM2OD1yLkiwPFM1KM7:TR?= MV7TRW5ITVJ?
C-33-A NEXjWI5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV3RTVc4UUN3ME2wMlgyOjl5IN88US=> NYf0SpVFW0GQR1XS
Calu-3 NIr0cY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2XrUmlEPTB;MD64NVQ{PCEQvF2= M3GzRXNCVkeHUh?=
AGS NVXHdWRkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M33MWGlEPTB;MD64N|Y1QCEQvF2= NXe3eo9[W0GQR1XS
SCC-25 MoLoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUXJR|UxRTBwOESxO|Eh|ryP NXyzR5FbW0GQR1XS
JVM-2 NGi4TWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlT2TWM2OD1yLki4Nlk6KM7:TR?= NH\m[lRUSU6JRWK=
HCE-T MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn\5TWM2OD1yLki4OFI5KM7:TR?= NX\NZ2J5W0GQR1XS
ES4 NX\hTJRkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGTTXpJKSzVyPUCuPVc5OzZizszN MYnTRW5ITVJ?
EKVX MoXSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mo\NTWM2OD1zLkCxNFY1KM7:TR?= NWXoVIZmW0GQR1XS
D-542MG NGHobGNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXjjSFE5UUN3ME2xMlAyODZ2IN88US=> MoLMV2FPT0WU
SW1116 MmS1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH;Ie45KSzVyPUGuNFEzQDdizszN M4TtN3NCVkeHUh?=
5637 M3rsWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGjPbplKSzVyPUGuNFE2OSEQvF2= NVy2OYhtW0GQR1XS
HT-1376 NGOySmdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXzJR|UxRTFwMEG1NVMh|ryP NYrCT4lSW0GQR1XS
NB69 MlrCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3LEUWlEPTB;MT6wNlk1PSEQvF2= M{\qSXNCVkeHUh?=
YKG-1 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGflV2tKSzVyPUGuNFU1OTlizszN M3TxcXNCVkeHUh?=
EW-3 NFrQfZlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVvJR|UxRTFwMEe4PFMh|ryP MYrTRW5ITVJ?
GCIY NGDvNlJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{TZZ2lEPTB;MT6wPFExPCEQvF2= Ml3CV2FPT0WU
SK-MES-1 NFuxcVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHrYUmFKSzVyPUGuNVE1QDdizszN NIHvTW9USU6JRWK=
PANC-08-13 MofpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlfpTWM2OD1zLkG2OFE3KM7:TR?= MXTTRW5ITVJ?
SK-NEP-1 MmrSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{XiN2lEPTB;MT6xO|UyOyEQvF2= NFvuPY9USU6JRWK=
SW954 MoDSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3HOS2lEPTB;MT6yNFIyPCEQvF2= MXfTRW5ITVJ?
NCI-SNU-5 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHvKc2lKSzVyPUGuNlM6KM7:TR?= M3rMV3NCVkeHUh?=
SW48 NFrWZYZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnvvTWM2OD1zLkK1OFMzKM7:TR?= MnvBV2FPT0WU
HT-3 NUPLV5BzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVvZOnlEUUN3ME2xMlI5PzV|IN88US=> MoP5V2FPT0WU
ETK-1 NYX6TlB2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWnJfHI4UUN3ME2xMlM4PDV4IN88US=> Mk[3V2FPT0WU
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NCI-H727 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUTJR|UxRThwMUC0NkDPxE1? MoPGV2FPT0WU
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HAL-01 M1L6PWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX\JR|UxRThwNUG5NUDPxE1? MVPTRW5ITVJ?
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SF539 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVHJR|UxRTlwMkO1OlIh|ryP MWnTRW5ITVJ?
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NCI-H1703 NX:0OXZXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXuxTpY4UUN3ME25MlM1Ozd3IN88US=> M1zaWHNCVkeHUh?=
NCI-H1650 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUe2WFV3UUN3ME25MlYxQTR4IN88US=> Mkn1V2FPT0WU
NCI-H1563 Mn;MS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlqyTWM2OD17Lke2NFMyKM7:TR?= NUjZcGxyW0GQR1XS
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OVCAR-4 MmThS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MY\JR|UxRTFyLki4N|Uh|ryP NXXJN4RTW0GQR1XS
CAL-72 MnW4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MY\JR|UxRTFyLkmzPVEh|ryP NHzneZVUSU6JRWK=
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SW626 M1ewd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWLJR|UxRTFzLkW3OVUh|ryP M2jKbHNCVkeHUh?=
RPMI-2650 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{Lac2lEPTB;MUGuOVg5PSEQvF2= NHPDOWhUSU6JRWK=
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HOP-62 NIjlTGJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NY\MPGRvUUN3ME2xNU43PzZ2IN88US=> M13rVHNCVkeHUh?=
NOS-1 NYq3bXpKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{DjSmlEPTB;MUGuO|ExQSEQvF2= NIPofFNUSU6JRWK=
SW1573 NXzmSWVST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmTPTWM2OD1zMT63Nlk2KM7:TR?= NHHXRYJUSU6JRWK=
WM-115 NGXNeVhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV;QTWZiUUN3ME2xNU44PTh6IN88US=> MXzTRW5ITVJ?
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MDA-MB-453 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUnXe|lYUUN3ME2xNU46PTF3IN88US=> NXn4bmR1W0GQR1XS
NCI-H596 M2rET2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXzoW5lwUUN3ME2xNk4xOzZ7IN88US=> NIHvfplUSU6JRWK=
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RS4-11 MmfNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVTMNGY2UUN3ME2xNk4yPzJ3IN88US=> NVzXNFVuW0GQR1XS
HCC1937 NH3CVm1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWf3N3ZzUUN3ME2xNk44PTZ2IN88US=> M37VXnNCVkeHUh?=
SW837 MnLGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{G1cmlEPTB;MUKuPVAzQCEQvF2= MkHBV2FPT0WU
NMC-G1 MlrES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2L5O2lEPTB;MUKuPVI{PCEQvF2= NWG3WoVOW0GQR1XS
PFSK-1 NHTHUZVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIO2fVJKSzVyPUGzMlA3KM7:TR?= NGH4fZlUSU6JRWK=
DBTRG-05MG MnzSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{DIVWlEPTB;MUOuNFY{OiEQvF2= NXvncog3W0GQR1XS
SK-UT-1 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVj0VZEyUUN3ME2xN{4yODh|IN88US=> MUTTRW5ITVJ?
OVCAR-5 MoftS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoPFTWM2OD1zMz6xOVk{KM7:TR?= MV;TRW5ITVJ?
8505C NUXCS4dJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYnJR|UxRTF|LkKwOlch|ryP NIPrfYtUSU6JRWK=
SK-MEL-1 MoHFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmHUTWM2OD1zMz63NVM2KM7:TR?= NEjyPXdUSU6JRWK=
LK-2 NE\yTYhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MljNTWM2OD1zMz63OlM6KM7:TR?= NIqwS3lUSU6JRWK=
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EW-13 MkK3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnPETWM2OD1zND6wN|UzKM7:TR?= Mo\qV2FPT0WU
NCI-H1793 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2TKfmlEPTB;MUSuNlA5PyEQvF2= M3XofXNCVkeHUh?=
BHT-101 Mnz5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml7RTWM2OD1zND6yOFEh|ryP MnHJV2FPT0WU
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NEC8 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV;JR|UxRTF2LkW0O|Ih|ryP NF;iSnJUSU6JRWK=
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NCI-H2030 NUnNZWlpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1ny[WlEPTB;MUSuOlQ1QCEQvF2= Mk\1V2FPT0WU
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A2058 M{HsSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXTJR|UxRTF3LkC0NFkh|ryP NWHmO41NW0GQR1XS
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J82 M{PwUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mme0TWM2OD1zNT6wOVch|ryP NWX1eINyW0GQR1XS
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COLO-824 NIDaWVFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml3oTWM2OD1zNT6xOVc6KM7:TR?= M2\ZO3NCVkeHUh?=
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OVCAR-8 M4DZcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWjTZXR[UUN3ME2xOU45QDZzIN88US=> NVfzZ|VKW0GQR1XS
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MHH-NB-11 M{XBWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWjDcVQyUUN3ME2xOk4xQDN7IN88US=> MV;TRW5ITVJ?
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NCI-H1092 NXe1eVF7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX7JR|UxRTF4LkizNVYh|ryP MYLTRW5ITVJ?
MZ2-MEL MlvVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NESw[4JKSzVyPUG2Mlg1ODNizszN M1vYdXNCVkeHUh?=
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COLO-684 M3j2dWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M13RPGlEPTB;MU[uPVQ4PyEQvF2= NXXDXXBJW0GQR1XS
NCI-H661 MmH2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{PROmlEPTB;MU[uPVY6PCEQvF2= MVrTRW5ITVJ?
CP50-MEL-B MmKxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV3JR|UxRTF5LkCzOFMh|ryP MWnTRW5ITVJ?
NCI-H2405 Mlr1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MofqTWM2OD1zNz6xPVc1KM7:TR?= MV3TRW5ITVJ?
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NCI-H2342 Ml3tS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1jHXGlEPTB;MUeuOVU4KM7:TR?= NXf4R2t6W0GQR1XS
ES8 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NY\LellZUUN3ME2xO{43ODh5IN88US=> NUf4d2NIW0GQR1XS
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RH-18 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2PaUmlEPTB;MUiuNFA{PSEQvF2= MWPTRW5ITVJ?
D-283MED M4jv[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{flcWlEPTB;MUiuNFA5QCEQvF2= M2PuVXNCVkeHUh?=
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NCI-H1437 NGq0bWhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NULGTGIxUUN3ME2xPE4yPjRzIN88US=> MmTtV2FPT0WU
SW982 NH7zemJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkHOTWM2OD1zOD6yOlcyKM7:TR?= MlTSV2FPT0WU
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SCH NXTDZ|dMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYXJR|UxRTF6Lk[0OVYh|ryP NYm0S284W0GQR1XS
COLO-320-HSR MkK4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3LKW2lEPTB;MUiuO|Q3KM7:TR?= MmfGV2FPT0WU
Mo-T MljQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWfxVYIyUUN3ME2xPE45OjB2IN88US=> MXjTRW5ITVJ?
Saos-2 M4\jVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV3i[opPUUN3ME2xPU4yOTZ{IN88US=> NVjiRlJHW0GQR1XS
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MMAC-SF M1fIUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYnXNms5UUN3ME2xPU4zPjB5IN88US=> NFfw[VZUSU6JRWK=
JAR MkH0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHy1fZpKSzVyPUG5MlI6QSEQvF2= MULTRW5ITVJ?
SK-MEL-28 NFK3flhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV:5S49jUUN3ME2xPU4{OzZ|IN88US=> M3zlbXNCVkeHUh?=
KNS-81-FD M3O2d2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWLJR|UxRTF7LkW0Olgh|ryP MXXTRW5ITVJ?
ES7 NFLXdlNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmnWTWM2OD1zOT62NlkzKM7:TR?= NG\4W2JUSU6JRWK=
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NCI-H1155 M2n0Xmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1HKNWlEPTB;MUmuO|I{QCEQvF2= Mn7LV2FPT0WU
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PC-3 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYW4bpRxUUN3ME2xPU46PjB2IN88US=> M2\TZnNCVkeHUh?=
GCT NUDhc|FpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXXJR|UxRTJyLkOwNlYh|ryP NH\YeY9USU6JRWK=
KARPAS-299 NWrJZ3E6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHrmTodKSzVyPUKwMlM1OjJizszN NXjTR21oW0GQR1XS
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COLO-741 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3jBR2lEPTB;MkCuOlYh|ryP M3zBc3NCVkeHUh?=
NCI-H1395 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVXOVng3UUN3ME2yNE43Pjl5IN88US=> MXnTRW5ITVJ?
HCC1187 NYi3bnJRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4f2fGlEPTB;MkCuOlc{PCEQvF2= MYPTRW5ITVJ?
Becker MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1XqTGlEPTB;MkCuO|IxOSEQvF2= MXTTRW5ITVJ?
LU-134-A M{DsfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkjaTWM2OD1{MT6xNFI3KM7:TR?= NXvvcno5W0GQR1XS
MKN45 Mo\zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnfnTWM2OD1{MT6xOVUyKM7:TR?= MXfTRW5ITVJ?
LC-2-ad MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlLJTWM2OD1{MT6yO|I6KM7:TR?= NH:yXIdUSU6JRWK=
EW-24 NFzoUlNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV7JR|UxRTJzLkO5OFUh|ryP M37TUXNCVkeHUh?=
SK-N-AS NVXaRWxoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVHJR|UxRTJzLkiyO|Yh|ryP M2j2RnNCVkeHUh?=
NCI-H2052 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoDkTWM2OD1{MT64PVY{KM7:TR?= NFXFZo5USU6JRWK=
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NB10 M4LPXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH;NdnpKSzVyPUKyMlI{PzFizszN MVfTRW5ITVJ?
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KM12 M2\3SGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkXKTWM2OD1{Mj6zOVYh|ryP M{exOXNCVkeHUh?=
JEG-3 NFzORnpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{fNVmlEPTB;MkKuN|c5KM7:TR?= NVLYb49YW0GQR1XS
LN-405 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH72d45KSzVyPUKyMlU6OTNizszN Mnj1V2FPT0WU
HCT-15 NU\BUlN4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVnBPHRZUUN3ME2yNk44PDRzIN88US=> Mon5V2FPT0WU
MLMA MoPLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWPJR|UxRTJ{Lke0O{DPxE1? NH;YfYRUSU6JRWK=
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RT-112 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkL5TWM2OD1{Mj64OlE3KM7:TR?= NHLtT5ZUSU6JRWK=
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A3-KAW MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlrvTWM2OD1{Mz6xNFA{KM7:TR?= NYXWOHREW0GQR1XS
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SF126 NHH6O4RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYfJR|UxRTJ|Lk[5NlQh|ryP Ml\oV2FPT0WU
SiHa MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1nDeGlEPTB;MkOuPVMzPyEQvF2= NH;6eGdUSU6JRWK=
MDA-MB-415 NV\oWFA6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIf3U2pKSzVyPUKzMlk1QTlizszN NGLPdYNUSU6JRWK=
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COR-L23 M2TsRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUnJR|UxRTJ2LkG3Olch|ryP Mm\zV2FPT0WU
KG-1 NFLVPGhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1\SO2lEPTB;MkSuNVkzQCEQvF2= MlnEV2FPT0WU
L-428 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYnNWJIyUUN3ME2yOE42PzlzIN88US=> MnrtV2FPT0WU
NCI-H520 Ml7qS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoW4TWM2OD1{ND64PFYyKM7:TR?= NXq5UG5zW0GQR1XS
COLO-792 NVexbZBTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUPJR|UxRTJ3LkK3N|Ih|ryP MXLTRW5ITVJ?
NB12 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVS5ZmtCUUN3ME2yOU4zQTR5IN88US=> M3zQUHNCVkeHUh?=
GAK MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn;mTWM2OD1{NT6yPVY{KM7:TR?= MoL2V2FPT0WU
SW780 M1P6SWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{XDOWlEPTB;MkWuN|I3PCEQvF2= MXPTRW5ITVJ?
GB-1 MmLRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2LTOWlEPTB;MkWuOFA3QSEQvF2= MXXTRW5ITVJ?
P12-ICHIKAWA M2\Hc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3PaVmlEPTB;MkWuOFc5PSEQvF2= NXe0empJW0GQR1XS
NCI-H1651 NWm5WWhST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4rMSWlEPTB;MkWuOVk4KM7:TR?= MVPTRW5ITVJ?
KE-37 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIX1TmVKSzVyPUK1Mlc{OTVizszN MV3TRW5ITVJ?
CCRF-CEM Ml7CS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWHJR|UxRTJ3Lkm2O{DPxE1? M{DxV3NCVkeHUh?=
SF295 NGLOfXJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{LwR2lEPTB;Mk[uNFQzPSEQvF2= M3voNXNCVkeHUh?=
G-361 M2[zWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVjhZnRbUUN3ME2yOk4yODJ5IN88US=> NH\iWoJUSU6JRWK=
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UMC-11 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHrWcXBKSzVyPUK2MlQ2OTRizszN NWi5O4Q6W0GQR1XS
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TE-11 MoHNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWWx[pF5UUN3ME2yOk44PzV{IN88US=> MoLoV2FPT0WU
NCI-H1304 NHzZRmpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{C1VWlEPTB;Mk[uPVExQCEQvF2= NEe2TZVUSU6JRWK=
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BCPAP MmTVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{LKbmlEPTB;MkeuNVM4KM7:TR?= M2jlWHNCVkeHUh?=
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EW-16 NFexSWpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{PZTmlEPTB;MkeuNlk1PyEQvF2= NI\lOGNUSU6JRWK=
HT-144 NYXR[WpoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1v5OmlEPTB;MkeuOFA{OiEQvF2= NIjL[oFUSU6JRWK=
MHH-ES-1 NIfqPI1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWTJR|UxRTJ5LkS0OFch|ryP MY\TRW5ITVJ?
T98G NHnoTIVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWnBRYp4UUN3ME2yO{42QDR4IN88US=> Mlv4V2FPT0WU
RPMI-8866 NHfT[oJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnTHTWM2OD1{Nz63OVYzKM7:TR?= NWnVTot6W0GQR1XS
BEN Mn;zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoH4TWM2OD1{Nz64OVE4KM7:TR?= MYLTRW5ITVJ?
MDA-MB-231 M1y3Wmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUDJR|UxRTJ6LkK3PVUh|ryP NFHkSppUSU6JRWK=
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SK-N-FI MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXzJR|UxRTJ6LkSzN{DPxE1? NIfT[lhUSU6JRWK=
AN3-CA MkX4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1;6NmlEPTB;MkiuOFM6PSEQvF2= MkT3V2FPT0WU
EGI-1 NYDUSGdDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnzOTWM2OD1{OD61PVI5KM7:TR?= NXHnem43W0GQR1XS
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TCCSUP Mn[3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3S1XGlEPTB;MkiuOlQyQCEQvF2= Ml3VV2FPT0WU
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SK-N-DZ NF3qSJpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1;GbGlEPTB;MkiuOlg5PSEQvF2= M3nuOHNCVkeHUh?=
MIA-PaCa-2 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWXJR|UxRTJ6Lke5OVkh|ryP NV;ZZZZWW0GQR1XS
IGR-1 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX;0S|lmUUN3ME2yPE45QDd2IN88US=> NVGwWJNKW0GQR1XS
BFTC-905 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYD6TmxkUUN3ME2yPU4zPzB3IN88US=> NYHxVYd2W0GQR1XS
NCI-H1581 M3ToTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUHJR|UxRTJ7LkOxOFUh|ryP M3vqWnNCVkeHUh?=
RD MkPGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWrjbllwUUN3ME2yPU42OjN|IN88US=> MWrTRW5ITVJ?
SF268 NGnzbZZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3z5V2lEPTB;MkmuOVk{OSEQvF2= M2LnNXNCVkeHUh?=
SHP-77 NFXWbZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MULJR|UxRTJ7Lk[xPFIh|ryP Mny5V2FPT0WU
D-566MG MlPXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnfrTWM2OD1{OT65N|k{KM7:TR?= M3O3RnNCVkeHUh?=
TGBC1TKB NU\uUXVLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2XFNmlEPTB;M{CuNVI{QCEQvF2= M1PaR3NCVkeHUh?=
MEL-HO MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGK2NWZKSzVyPUOwMlE1OjNizszN MUnTRW5ITVJ?
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D-336MG M4fIV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3;mNWlEPTB;M{CuNlg6OiEQvF2= NIH3S4VUSU6JRWK=
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Calu-6 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF3N[oRKSzVyPUOwMlI6PjNizszN MnHOV2FPT0WU
MZ1-PC MnPoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{PGV2lEPTB;M{CuN|k{KM7:TR?= NGiySYdUSU6JRWK=
KYSE-70 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mny2TWM2OD1|MD61NVE1KM7:TR?= MkPLV2FPT0WU
CAPAN-1 M2nuT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkDCTWM2OD1|MD61NVgyKM7:TR?= M2jZVnNCVkeHUh?=
DK-MG NUTpVpBWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGXlWYxKSzVyPUOwMlU1PTZizszN NF7nOnBUSU6JRWK=
SK-HEP-1 M{nHdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mkf0TWM2OD1|MD62N|I3KM7:TR?= NULlXIF7W0GQR1XS
MS-1 NWC5T4J2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mo\oTWM2OD1|MD64PFg6KM7:TR?= MkXZV2FPT0WU
GI-ME-N NHnKPYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYLJR|UxRTNzLkC1O|Eh|ryP Mk\YV2FPT0WU
NCI-H510A Ml\pS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2jEdmlEPTB;M{GuOFAxPiEQvF2= NYnTcmEzW0GQR1XS
NB17 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NE\DfY1KSzVyPUOxMlUyQDVizszN NH76[oFUSU6JRWK=
639-V NV3zd5R4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGHOPHBKSzVyPUOxMlUzPDNizszN MnqzV2FPT0WU
CAL-51 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnXDTWM2OD1|MT61OlY4KM7:TR?= MXnTRW5ITVJ?
HGC-27 NUPYOndxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWXwVoM3UUN3ME2zNU42QDd|IN88US=> NXPNcIRUW0GQR1XS
GOTO NEfCUXNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH;SS3FKSzVyPUOxMlg2ODZizszN MXPTRW5ITVJ?
ChaGo-K-1 MnfoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkLoTWM2OD1|Mj6wNFch|ryP NU\WcWk3W0GQR1XS
RO82-W-1 NGTndnNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{n0VGlEPTB;M{KuNVYxPCEQvF2= M{P6UXNCVkeHUh?=
SH-4 NEDPe5VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkjiTWM2OD1|Mj6xPFU5KM7:TR?= MVvTRW5ITVJ?
A204 NITNbo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NU\yS3doUUN3ME2zNk42ODZ7IN88US=> NVnqRWhOW0GQR1XS
DMS-273 NIXxWpJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUfJR|UxRTN{LkWzNlIh|ryP NF7HRnRUSU6JRWK=
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SK-MEL-2 M1i1S2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1;SXWlEPTB;M{KuOlUzPyEQvF2= M1q2NXNCVkeHUh?=
NCI-H2228 M4HkVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkT4TWM2OD1|Mj62OlAzKM7:TR?= Mn3QV2FPT0WU
MKN28 MlriS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmX5TWM2OD1|Mj63PFM5KM7:TR?= MUnTRW5ITVJ?
H-EMC-SS NVPmZXBnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4fQSWlEPTB;M{KuPVY4QCEQvF2= M1;GbnNCVkeHUh?=
ATN-1 MkfQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3XORWlEPTB;M{OuNVQyPiEQvF2= NFPDN4hUSU6JRWK=
D-263MG MkjQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUnJR|UxRTN|LkizO|gh|ryP NV[yfnBuW0GQR1XS
LU-135 Ml3SS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFH0W4NKSzVyPUOzMlg5OjlizszN NGX0ZplUSU6JRWK=
C8166 MmLtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mk\oTWM2OD1|Mz64PFU1KM7:TR?= MoXnV2FPT0WU
T-24 MmO1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWXpUYJLUUN3ME2zOE4xOjB3IN88US=> MkP4V2FPT0WU
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22RV1 Mlm2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYf2fpUyUUN3ME2zOE4yPzV2IN88US=> Ml3PV2FPT0WU
KM-H2 NGHWVGZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYrJR|UxRTN2LkG3O|kh|ryP NI\kOWZUSU6JRWK=
NCI-H2122 NVvNRYF7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXfodYxtUUN3ME2zOE4{PDdzIN88US=> NITYenVUSU6JRWK=
ABC-1 NU\jbZQzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVLGXpRnUUN3ME2zOE4{Pjl|IN88US=> M1fadHNCVkeHUh?=
NCI-H1355 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoqzTWM2OD1|ND6zPFg6KM7:TR?= M1i3OHNCVkeHUh?=
NOMO-1 NVrSUm1jT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1W3dGlEPTB;M{SuOVcxOyEQvF2= NYDJfoV5W0GQR1XS
MZ7-mel MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4L4R2lEPTB;M{SuOlQ5PyEQvF2= NGO2dXJUSU6JRWK=
NCI-H810 M1[wU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4jwRmlEPTB;M{SuO|I4QCEQvF2= NHPtdIJUSU6JRWK=
D-392MG MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV\JR|UxRTN3LkC1OlUh|ryP NYHKc2IzW0GQR1XS
LB771-HNC NYrOd|hCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXjleJkyUUN3ME2zOU4yPjV6IN88US=> MU\TRW5ITVJ?
COLO-679 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1e2ZmlEPTB;M{WuN|g5KM7:TR?= NGmzXVBUSU6JRWK=
SK-PN-DW NGexSWtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIq5XHhKSzVyPUO1MlQ{PjlizszN MoO0V2FPT0WU
VA-ES-BJ NWnTbo9qT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX\JR|UxRTN3LkS2OFkh|ryP M3y0bXNCVkeHUh?=
KNS-42 MmnqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUHqfXIzUUN3ME2zOU42QDJ4IN88US=> NUW2SXhuW0GQR1XS
RVH-421 NX;Ie5lUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NY\HT41iUUN3ME2zOU44PjR3IN88US=> NXPsOGcxW0GQR1XS
P30-OHK MmHnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2OwVWlEPTB;M{WuPFc2OiEQvF2= NETaNHRUSU6JRWK=
G-402 NV\Z[mxLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYXXbWZxUUN3ME2zOU46QDZ5IN88US=> Ml;tV2FPT0WU
S-117 NF3BfVhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mk\FTWM2OD1|Nj6zNFA5KM7:TR?= MVHTRW5ITVJ?
RXF393 NWnDPFJ1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWPHTlJXUUN3ME2zOk4{Pzh3IN88US=> NWjOTIU6W0GQR1XS
LCLC-103H NV\KfWF2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn61TWM2OD1|Nj63PFA{KM7:TR?= MnvPV2FPT0WU
U-118-MG NVLGV4x4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVrJR|UxRTN4Lki4OFch|ryP NX7FcXVVW0GQR1XS
8-MG-BA M3\mPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV;JR|UxRTN4Lkm0PFMh|ryP M4PmcXNCVkeHUh?=
MFH-ino MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWjJR|UxRTN5LkOzO|Eh|ryP MXHTRW5ITVJ?
NCI-H23 M3r2W2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWTJR|UxRTN5LkSxPVYh|ryP M3LPU3NCVkeHUh?=
NCI-H446 M1LRXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mm\STWM2OD1|Nz60PFYzKM7:TR?= MWnTRW5ITVJ?
LU-99A NEThTWhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoDCTWM2OD1|Nz61O|MyKM7:TR?= NE\rbnZUSU6JRWK=
KP-N-YN MmrMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1:1TGlEPTB;M{euOlE6PSEQvF2= M1m3[XNCVkeHUh?=
SCC-9 M{D1TGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NI[4TYRKSzVyPUO3Mlc5OzhizszN MknrV2FPT0WU
EFM-19 Ml\SS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3HlXWlEPTB;M{euO|k3PyEQvF2= M2S4[3NCVkeHUh?=
A427 MoP0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX7JR|UxRTN5LkmxNFch|ryP MYTTRW5ITVJ?
LB831-BLC Ml;aS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFvT[mVKSzVyPUO4MlYxOzlizszN NFTLNnNUSU6JRWK=
HOS MoDES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mlu0TWM2OD1|OT62Nlc{KM7:TR?= MYfTRW5ITVJ?
LAN-6 NXjjUXJsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH3ZOYNKSzVyPUSwMlAyOTJizszN MX3TRW5ITVJ?
U251 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoDsTWM2OD12MD60NFU1KM7:TR?= MULTRW5ITVJ?
SNU-387 NYXWUXpjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlToTWM2OD12MD60OVU{KM7:TR?= MWfTRW5ITVJ?
DMS-79 NE\kNnJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkHrTWM2OD12MD61PFg6KM7:TR?= MWfTRW5ITVJ?
SK-LU-1 NFXoOJpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXXrXWgyUUN3ME20NE43PTZ6IN88US=> MXPTRW5ITVJ?
HT NX;JWnJlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUfSV|dOUUN3ME20NE43PjN7IN88US=> MYXTRW5ITVJ?
KP-N-YS NEPr[5RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NU[5OG54UUN3ME20NE43PzZ6IN88US=> Mn;TV2FPT0WU
DMS-114 NYC5WZJsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEP3dpNKSzVyPUSwMlg{PzRizszN NHrpcGFUSU6JRWK=
HUTU-80 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{f4bGlEPTB;NEGuNFU3QSEQvF2= MlPPV2FPT0WU
ONS-76 NFr1UY5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXHYNHRSUUN3ME20NU41PzB7IN88US=> M1XMOHNCVkeHUh?=
A673 NEntNGdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYPBfXZmUUN3ME20NU42OTV5IN88US=> MYTTRW5ITVJ?
HPAF-II M{LWPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1XtZ2lEPTB;NEGuOlcxQSEQvF2= M{m0UXNCVkeHUh?=
UACC-257 MkfyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXu2VHBEUUN3ME20NU44OzZ7IN88US=> MYPTRW5ITVJ?
EW-22 NITTZ2hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkPRTWM2OD12MT64Olk4KM7:TR?= MWHTRW5ITVJ?
NCI-H1755 M3TmNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFLZPFdKSzVyPUSxMlg4OjVizszN NECxXFhUSU6JRWK=
KYSE-150 M3LxNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHO0RnlKSzVyPUSyMlE4QDJizszN MXfTRW5ITVJ?
CGTH-W-1 NGrMPWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUXJR|UxRTR{LkS5OVUh|ryP NYPkOIJNW0GQR1XS
NB13 M1K4[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYnYboVCUUN3ME20Nk43OjF5IN88US=> MY\TRW5ITVJ?
SW872 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M37BW2lEPTB;NEKuOlM{OSEQvF2= MVfTRW5ITVJ?
KP-4 M4n6RWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml36TWM2OD12Mj64PFMzKM7:TR?= NYDuZ3c1W0GQR1XS
HCC38 M3P0T2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWHKVmdVUUN3ME20Nk46PjF6IN88US=> MkfzV2FPT0WU
BB49-HNC NWHjXGxuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHHDVGNKSzVyPUSzMlI{PTZizszN M2i1OXNCVkeHUh?=
CAL-62 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYrJR|UxRTR|LkSwNlgh|ryP NEXZSpJUSU6JRWK=
NCI-H2029 NVfWfmloT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn70TWM2OD12Mz61OVgyKM7:TR?= M2jjSnNCVkeHUh?=
M14 NEW2eJJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIDO[FJKSzVyPUS0MlE{OzJizszN NYjuSmhCW0GQR1XS
COLO-680N NVPxWXBxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV7JR|UxRTR2LkOzPUDPxE1? MmXZV2FPT0WU
Hs-578-T M4fJ[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIL5SGhKSzVyPUS1MlAzQTRizszN MWHTRW5ITVJ?
MSTO-211H NIO2eoNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MU\JR|UxRTR3Lkm0PFIh|ryP NH7rPXRUSU6JRWK=
EW-1 M2HON2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIf3ZWlKSzVyPUS1Mlk3QDlizszN NWeyeHJKW0GQR1XS
SW948 Mk\2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVzJR|UxRTR4LkS0OlIh|ryP M{TEOXNCVkeHUh?=
NCI-H460 Mo\GS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVPJR|UxRTR4LkWyN{DPxE1? MUHTRW5ITVJ?
CAS-1 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlL5TWM2OD12Nj63O|AyKM7:TR?= NEfRdXNUSU6JRWK=
TGBC11TKB NXnNbVNVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4ru[GlEPTB;NEeuNVc4PyEQvF2= NE\xR5VUSU6JRWK=
HD-MY-Z MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEj0bo9KSzVyPUS4Mlc5PiEQvF2= NF76UIpUSU6JRWK=
NCI-H226 M1vm[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFX1OZFKSzVyPUS5Mlc4QDVizszN MWfTRW5ITVJ?
PA-1 MmT5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEDxZW1KSzVyPUS5Mlg{OzVizszN NXzsSHFPW0GQR1XS
LB2518-MEL NG\mNnpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkHJTWM2OD12OT64O|I{KM7:TR?= M2PTUXNCVkeHUh?=
A101D NHXvSVZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MneyTWM2OD12OT65OFI2KM7:TR?= NIXu[4VUSU6JRWK=
NCI-H2087 MmSxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVzJR|UxRTR7Lkm2PFIh|ryP MXXTRW5ITVJ?

... Click to View More Cell Line Experimental Data

In vivo Daily oral administration of BIBW2992 at 20 mg/kg for 25 days results in dramatic tumor regression with a cumulative treated/control tumor volume ratio (T/C ratio) of 2%. Reduced phosphorylation of EGFR and AKT is confirmed by immunohistochemical staining of tissue sections. Therefore, like lapatinib and neratinib, BIBW2992 is a next generation tyrosine kinase inhibitor (TKI) that inhibits human epidermal growth factor receptor 2 (Her2) and epidermal growth factor receptor (EGFR) kinases irreversibly. BIBW2992 is not only active against EGFR mutations targeted by first generation TKIs like Erlotinib or Gefitinib, but also against those insensitive to these standard therapies. [1]

Protocol

Kinase Assay:

[1]

+ Expand

In vitro kinase activity assays:

The wild type tyrosine kinase domain of the human EGFR as well as that of the EGFR L858R/T790M double mutant are fused to Glutathione-S-transferase (GST), and extracted. Enzyme activity is then assayed in the presence of the inhibitor BIBW2992, serially diluted in 50% DMSO. A random polymer pEY (4:1) is used as substrate and biotinylated pEY (bio-pEY) is added as a tracer substrate. The kinase domain of HER2 is cloned using the baculovirus system and extracted similarly to that of EGFR kinase. Details of assays for EGFR, HER2, SRC, BIRK and VEGFR2 kinase activity are available in Supplementary information.
Cell Research:

[1]

+ Expand
  • Cell lines: NSCLC cells
  • Concentrations: 0-10 μM
  • Incubation Time: 1 hour
  • Method:

    1 × 104 NSCLC cells are transferred into each well of a 96-well plate and cultured overnight in serum-free media for the EGFR phosphorylation assay. After addition of BIBW2992 on the next day, the plates are incubated at 37 °C for 1 hour. EGF-stimulation is done using 100 ng/mL for 10 min at room temperature. Cells are washed with ice cold PBS, extracted with 120 μL HEPEX buffer per well and shaken at room temperature for 1 hour. In all 2 × 104 cells per well is used for the HER2 phosphorylation assay. Streptavidin precoated plates are coated with anti-EGFR-biotin at 1:100 dilution in blocking buffer and c-erb2/HER2 oncoprotein Ab-5(Clone N24)-Biotin. Cell extracts is then transferred to the antibody-coated wells and incubated at room temperature for 1 hour. Extinction is measured at 450 nm.


    (Only for Reference)
Animal Research:

[1]

+ Expand
  • Animal Models: Athymic NMRI-nu/nu female mice
  • Formulation: In 0.5% methocellulose-0.4% polysorbate-80 (Tween 80)
  • Dosages: 20 mg/kg
  • Administration: Oral administration
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 97 mg/mL (199.61 mM)
Ethanol 15 mg/mL (30.86 mM)
Water Insoluble
In vivo Add solvents individually and in order:
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
10mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 485.94
Formula

C24H25ClFN5O3

CAS No. 439081-18-2
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02795156 Recruiting Non-small Cell Lung Carcinoma|Urothelial Carcinoma|Gastrointestinal Carcinoma, Non-colon|Upper Aerodigestive Tract Carcinoma SCRI Development Innovations, LLC|Foundation Medicine|Boehringer Ingelheim|Bayer September 28, 2016 Phase 2
NCT03054038 Not yet recruiting Non-Small Cell Lung Carcinoma Vanderbilt-Ingram Cancer Center|National Cancer Institute (NCI) February 2017 Phase 1
NCT02979977 Not yet recruiting Squamous Cell Cancers of the Head and Neck Aarti Bhatia|National Comprehensive Cancer Network|Boehringer Ingelheim|Yale University December 2016 Phase 2
NCT02876081 Not yet recruiting SMALL CELL LUNG CARCINOMA Groupe Hospitalier Paris Saint Joseph December 2016 Phase 2
NCT02183883 Recruiting Non-small Cell Lung Cancer University College, London|Boehringer Ingelheim December 2016 Phase 2
NCT02423525 Recruiting Brain Cancer Santosh Kesari|Boehringer Ingelheim|John Wayne Cancer Institute October 2016 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    What’s the recommendation about reconstitution of the compound for in vivo animal study?

  • Answer:

    For in vivo experiment of afatinib, we recommend 0.5% methylcellulose/0.2% Tween 80 as vehicle.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID