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SC66 Akt inhibitor

Cat.No.S5313

SC66 is an allosteric inhibitor which displays a dual-inhibitory function toward AKT activity with IC50 values of 0.77, 2.85 and 0.47 μg/ml in HepG2, Huh7 and Hep3B cells after 72 h treatment, respectively.
SC66 Akt inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 276.33

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Quality Control

Batch: S531301 DMSO]55 mg/mL]false]]]false]]]false Purity: 99.97%
99.97

Chemical Information, Storage & Stability

Molecular Weight 276.33 Formula

C18H16N2O

 Storage (From the date of receipt)
CAS No. 871361-88-5 -- Storage of Stock Solutions

Synonyms N/A Smiles C1CC(=CC2=CC=NC=C2)C(=O)C(=CC3=CC=NC=C3)C1

Solubility

In vitro
Batch:

DMSO : 55 mg/mL (199.03 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo
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In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Mechanism of Action

Targets/IC50/Ki
Akt [1]
In vitro
SC66 reduces cell viability in a dose- and time-dependent manner, inhibits colony formation and induces apoptosis in HCC cells. This compound treatment leads to a reduction in total and phospho-AKT levels. This is associated with alterations in cytoskeleton organization, a reduction in expression levels of E-cadherin, β-catenin and phospho-FAK, together with up-regulation of Snail protein levels. In addition, it induces the production of reactive oxygen species (ROS) and DNA damage. It affects AKT/mTOR signaling in HCC cell lines[1].
In vivo
In the mouse xenograft tumor model of Hep3B cells, SC66 treatment significantly reduces tumor volume to 37% on day 17 of treatment when compared with tumors in the untreated group. The inhibition of cell growth correlates with a reduction in phospho-AKT levels in the tumors of animals treated with this compound[1].
References

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