AZD5363

Catalog No.S8019

AZD5363 Chemical Structure

Molecular Weight(MW): 428.92

AZD5363 potently inhibits all isoforms of Akt(Akt1/Akt2/Akt3) with IC50 of 3 nM/8 nM/8 nM in cell-free assays, similar to P70S6K/PKA and lower activity towards ROCK1/2. Phase 2.

Size Price Stock Quantity  
In DMSO USD 235 In stock
USD 147 In stock
USD 470 In stock

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3 Customer Reviews

  • G-H. Analysis of cells treated with AZD5363 (500 nM) for 24 hrs. G.Western analysis of whole cell lysates. H. Cells labeled with Annexin V-FITC for 6h were imaged. Individual points are average for replicates assessed in duplicate. Midlines are the average ± S.D. Student’s T-test.

    Cancer Res, 2016, 76(16):4752-64.. AZD5363 purchased from Selleck.

    Inhibition of PI3K and Akt by LY294002 and AZD5363 was confirmed by analyzing expression and activation status of the ribosomal protein S6 by Western blot analysis of PC-3 clone #14 cells treated with 0.5 or 5 uM AZD5363 (AZD) and 10 uM LY294002 (LY). Tubulin was used as a loading control.

    Cell Commun Signal 2014 12(1), 61. AZD5363 purchased from Selleck.

  • LNCaP, LNCaP95, VCaP and 22Rv1 cells were transfected with control or pooled siRNA for AKT 1–3 isoforms for 36 hours. Cells were then further treated with 5 uM AZD5363 (B) for another 18 hours. Protein lysates were immunoblotted with AR (N-20), AR-V7, Pan-AKT, p-AKT(ser473) and β-Actin antibodies. Results were repeated in more than three independent experiments.

    PLoS One 2014 9(10), e108780. AZD5363 purchased from Selleck.

Purity & Quality Control

Choose Selective Akt Inhibitors

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Notes:

2. For more details, such as half maximal inhibitory concentrations (IC50s) and working concentrations of each inhibitor, please click on the link of the inhibitor of interest.
3. "+" indicates inhibitory effect. Increased inhibition is marked by a higher "+" designation.
4. Orange "√" refers to compounds which do inhibitory effects on the related isoform, but without specific value.

Biological Activity

Description AZD5363 potently inhibits all isoforms of Akt(Akt1/Akt2/Akt3) with IC50 of 3 nM/8 nM/8 nM in cell-free assays, similar to P70S6K/PKA and lower activity towards ROCK1/2. Phase 2.
Features Moderate preclinical tolerability, and PD characteristics of an AKT inhibitor. Distinct profile from other AKT inhibitors in clinical development.
Targets
Akt1 [1]
(Cell-free assay)
Akt2 [1]
(Cell-free assay)
Akt3 [1]
(Cell-free assay)
ROCK2 [1]
(Cell-free assay)
3 nM 8 nM 8 nM 56 nM
In vitro

AZD5363 is a potent Akt inhibitor with IC50 of 3 nM, 8 nM and 8 nM for Akt1, Akt2 and Akt3, respectively. [1] AZD5363 inhibits phosphorylation of AKT substrates in cells with a potency of approximately 0.3 to 0.8 μM. AZD5363 inhibits the proliferation of 41 of 182 solid and hematologic tumor cell lines with a potency of < 3 μM. [2] Activating mutations in PIK3CA, loss or inactivation of tumor suppressor PTEN, or HER2 amplification all are significantly predictive of responsiveness to AZD5363. Additionally, correlation is also seen between the RAS mutation status of cell lines and resistance to AZD5363. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MCF7 M3LMfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkHTNlAxKG6P MlG5OkBl NYG5SnhocW6lcnXhd4VlKGS{dXegd4Vve2m2aY\peJkhd2ZiND3PTHQh[W6mIH\1cJZme3S{YX70 M4TPbVI3OzVzM{Kz
ZR75 NETVVnRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2r4OlExOCCwTR?= M37qPVYh\A>? MUXpcoNz\WG|ZXSg[JJ2\yC|ZX7zbZRqfmm2eTDv[kA1NU:KVDDhcoQh\nWudnXzeJJidnR? NEPwXHgzPjN3MUOyNy=>
T74D MnjNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVvYSHBOOTByIH7N NYn2fWpRPiCm M4rITIlv[3KnYYPl[EBlenWpIIPlcpNqfGm4aYT5JI9nKDRvT1jUJIFv\CCodXz2[ZN1emGwdB?= NXnyUIZPOjZ|NUGzNlM>
1%MCF7 NIS5Z3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVTuNYd{PDByIH7N MXy2JIQ> NHjE[5lqdmO{ZXHz[YQh\HK3ZzDz[Y5{cXSrdnn0fUBw\iB2LV;IWEBidmRiZoXseoV{fHKjboS= M2rWc|I3OzVzM{Kz
MCF7 LTED M1nlXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUeyNFAhdk1? NEH3OZo3KGR? MkHWbY5kemWjc3XkJIRzfWdic3Xud4l1cX[rdImgc4YhPC2RSGSgZY5lKG[3bI\ld5Rz[W62 MX2yOlM2OTN{Mx?=
ZR75 LTED Ml\sS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFe0SZEyODBibl2= NGDnSWg3KGR? Mn\mbY5kemWjc3XkJIRzfWdic3Xud4l1cX[rdImgc4YhPC2RSGSgZY5lKG[3bI\ld5Rz[W62 MoTSNlY{PTF|MkO=
T74D LTED M1XaPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MljrNVAxKG6P NG\6TFg3KGR? NVHr[ZdzcW6lcnXhd4VlKGS{dXegd4Vve2m2aY\peJkhd2ZiND3PTHQh[W6mIH\1cJZme3S{YX70 Mlm3NlY{PTF|MkO=
TamR MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWS0NFAhdk1? NYHNPGx4PiCm NF3sNVJqdmO{ZXHz[YQh\HK3ZzDz[Y5{cXSrdnn0fUBw\iB2LV;IWEBidmRiZoXseoV{fHKjboS= M1PvU|I3OzVzM{Kz
HCC1954 NYW1[XloT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH\CdpgxNTFwM{Wg{txO M2jTd|Uh\A>? Mmi1[Y5p[W6lZYOgeIhmKGe{b4f0bEBqdmirYnn0bY9vKG:oIFHaSFg6OzF? MWGyOlA6PTR5NR?=
BT474c M17kWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXuwMVEvOzVizszN MWe1JIQ> MknR[Y5p[W6lZYOgeIhmKGe{b4f0bEBqdmirYnn0bY9vKG:oIFHaSFg6OzF? MnfiNlYxQTV2N{W=
KPL4 M3XBTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NETvTFYxNTFwM{Wg{txO M3PTU|Uh\A>? M4Hr[oVvcGGwY3XzJJRp\SCpcn;3eIghcW6qaXLpeIlwdiCxZjDBXmQ5QTNz NVzPfFM4OjZyOUW0O|U>
SKBR3 NV7Yb3ExT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVLYdlJVOC1zLkO1JO69VQ>? MVy1JIQ> MlTw[Y5p[W6lZYOgeIhmKGe{b4f0bEBqdmirYnn0bY9vKG:oIFHaSFg6OzF? NYL0SnI{OjZyOUW0O|U>
MR49C M3;PdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3H5blAuPSEQvF2= M1;uW|Q5KGh? MXPpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uh\GWyZX7k[Y51KG2jbn7ldi=> MkPuNlUyPTFyMUK=
MR49F NX7VSFh1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3;sNVAuPSEQvF2= NH:zZoE1QCCq NX\MOWJucW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUBld3OnIHTldIVv\GWwdDDtZY5v\XJ? NFK3T|QzPTF3MUCxNi=>
NCI-H522 NHz1cYFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1viemlEPTB;MUGuN{ApyrF{LkepJO69VQ>? MmTLNlQ6PTd4OEK=
PC-9 MlTES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUjJR|UxRTlwMzCoxtEyNjJrIN88US=> NYDLSlczOjR7NUe2PFI>
NCI-H522 MlPNSpVv[3Srb36gRZN{[Xl? MYqxM|UwOTBizszN NUnEfZltPC9{NDDo NGLHTJlqdmO{ZXHz[ZMhSUuWIIDoc5NxcG:{eXzheIlwdg>? MlnaNlQ6PTd4OEK=
PC-9 MX7GeY5kfGmxbjDBd5NigQ>? NWP4RmJPOS93L{GwJO69VQ>? MVW0M|I1KGh? MmiybY5kemWjc3XzJGFMXCCyaH;zdIhwenmuYYTpc44> M1XVZVI1QTV5Nkiy
HGC27 MkjqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYfL[|RrUUN3ME2wMlQ1PSEQvF2= NEDOeFUzPDB6OEO4Ni=>
IM95m Mln6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVjJR|UxRTBwNUGg{txO NEfXbG4zPDB6OEO4Ni=>
AGS NFvWe4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV\lRZh3UUN3ME2wMlU2OiEQvF2= M2W0OVI1ODh6M{iy
NCI-N87 NFmxS3BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUTqW49iUUN3ME2xMlA{PyEQvF2= NGrkb|kzPDB6OEO4Ni=>
23132/87 NV7HcJczT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHrNU5dKSzVyPUGuOlcyKM7:TR?= MVeyOFA5QDN6Mh?=
MKN1 NHO1eGtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3foN2lEPTB;Mj60NlEh|ryP MlHUNlQxQDh|OEK=
SNU-620 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1;C[GlEPTB;Mz6zPFQh|ryP NILYNVUzPDB6OEO4Ni=>
SNU-638 NIrUeWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MULJR|UxRTRwNUKzJO69VQ>? MnnYNlQxQDh|OEK=
SNU-1 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEjxWYxKSzVyPUWuNlU5KM7:TR?= NY\jV|k3OjRyOEizPFI>
SNU-601 Mm\NS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3uxRmlEPTB;NT65N|gh|ryP MXeyOFA5QDN6Mh?=
SNU-668 MkL6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2POR2lEPTB;Nj6wNFMh|ryP M1jRblI1ODh6M{iy
HS746T NVvXVXpRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWHJR|UxRTZwMEi0JO69VQ>? M{XERVI1ODh6M{iy
KATO III M3jWZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVPJR|UxRTdwMk[3JO69VQ>? NWe0[IlEOjRyOEizPFI>
SNU-484 NH:3VY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4e2UWlEPTB;Nz6zPVIh|ryP MnHmNlQxQDh|OEK=
SNU-16 M3fKTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnnGTWM2OD1zMT6wPVch|ryP MnjPNlQxQDh|OEK=
OCUM-1 NWPBO2UzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX;5WoZEUUN3ME2xOE42OTVizszN NIW4WlMzPDB6OEO4Ni=>
NUGC-3 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYTJR|UxRTJzLki3N{DPxE1? NH3jR5EzPDB6OEO4Ni=>
AZ521 NHvWRplIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF76V3JKSzVyPUK1MlQ1QCEQvF2= NIjweogzPDB6OEO4Ni=>
SNU-216 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MULJR|UxRTNyIN88US=> NUXIW3BzOjRyOEizPFI>
NUGC-4 NGiyW5RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnrnTWM2OD1|MDFOwG0> MUOyOFA5QDN6Mh?=
SNU-5 M4S4V2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIfwZ3pKSzVyPUOwJO69VQ>? NHPLO4czPDB6OEO4Ni=>
GTL-16 NYf3eHptT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHfQTFRKSzVyPUOwJO69VQ>? M1\OeVI1ODh6M{iy
MKN74 NIHwNJNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3zjT2lEPTB;M{Cg{txO M2TBVlI1ODh6M{iy
PAMC82 NGnRS5VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlKyTWM2OD1|MDFOwG0> NVrHblhQOjRyOEizPFI>
LNCaP M3jSbWZ2dmO2aX;uJGF{e2G7 MorlOUDPxE1? NXPGTXNJOC1{NDDo Ml[zbY5lfWOnczDBT3RUPDd|IHHu[EBCU1SWM{C4JJBpd3OyaH;yfYxifGmxbjDpckBiKHSrbXWg[IVx\W6mZX70JI1idm6nch?= MmLMNlM6PjZ4MkG=
C4-2  NHO4SJVHfW6ldHnvckBCe3OjeR?= M3LwWVUh|ryP M3[zT|AuOjRiaB?= MnjVbY5lfWOnczDBT3RUPDd|IHHu[EBCU1SWM{C4JJBpd3OyaH;yfYxifGmxbjDpckBiKHSrbXWg[IVx\W6mZX70JI1idm6nch?= Mmj6NlM6PjZ4MkG=
LNCaP NFnObG5HfW6ldHnvckBCe3OjeR?= NYrhb3hSPSEQvF2= NYjKV5NYOC1{NDDo NWjYToN6cW6qaXLpeJMheGixc4Doc5J6dGG2aX;uJI9nKHSqZTDkbZN1[WxiQVvUMZBifGi5YYmgZolwdWG{a3Xyd{BqdmOudXTpcochWFKDU{SwMEBmUUZ2RTygOGUuSlBzLDDtWG9TNCCjbnSgVFcxKFN4IHvpcoF{\SCrbjDhJJRqdWVvZHXw[Y5l\W62IH3hco5meg>? NH\4UIUzOzl4Nk[yNS=>
C4-2  MYLGeY5kfGmxbjDBd5NigQ>? M1juTVUh|ryP M1\ISlAuOjRiaB?= NWnLO4tpcW6qaXLpeJMheGixc4Doc5J6dGG2aX;uJI9nKHSqZTDkbZN1[WxiQVvUMZBifGi5YYmgZolwdWG{a3Xyd{BqdmOudXTpcochWFKDU{SwMEBmUUZ2RTygOGUuSlBzLDDtWG9TNCCjbnSgVFcxKFN4IHvpcoF{\SCrbjDhJJRqdWVvZHXw[Y5l\W62IH3hco5meg>? MnHxNlM6PjZ4MkG=
LNCaP M3LaWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3rzWlEuOTByMECgcm0> NYj2UItbOC1|IHS= MoXZbY5pcWKrdIOgZ4VtdCC4aXHibYxqfHliZH;z[UBl\XCnbnTlcpRtgQ>? NHnJ[4IzOzl4Nk[yNS=>
C4-2  NY[x[Ix1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlPoNU0yODByMDDuUS=> NFjIcGYxNTNiZB?= NIDMSJVqdmirYnn0d{Bk\WyuII\pZYJqdGm2eTDkc5NmKGSncHXu[IVvfGy7 NWLBdIlWOjN7Nk[2NlE>
LNCaP NI\je4hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWexNFAuPTByMDDuUS=> NX7Y[HRXPzJiaB?= M3nKbIlv[3KnYYPld{B1cGViZoLhZ5Rqd25ib3[gZ4VtdHNidX7k[ZJod2mwZzDj[YxtKGSnYYTo M{T5SFI{QTZ4NkKx
C4-2  M3fvVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2jQ[VExOC13MECwJI5O MWK3NkBp MVHpcoNz\WG|ZYOgeIhmKG[{YXP0bY9vKG:oIHPlcIx{KHWwZHXy[49qdmdiY3XscEBl\WG2aB?= NXraRnFqOjN7Nk[2NlE>
PC-3 MknISpVv[3Srb36gRZN{[Xl? NWX5eI1HOC53L{GvNVAh|ryP MXG0PEBp NXvBOFZv\G:5boLl[5Vt[XSnczD0bIUheGixc4Doc5J6dGG2aX;uJI9nKGSxd37zeJJm[W1icHH0bJdigSCycn;0[YlveyCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? MkXLNlMzPTh5NEC=
DU145  NULUTFlMTnWwY4Tpc44hSXO|YYm= MlLQNE42NzFxMUCg{txO M2rGSVQ5KGh? MUnkc5dvemWpdXzheIV{KHSqZTDwbI9{eGixconsZZRqd25ib3[g[I94dnO2cnXhcUBx[XSqd3H5JJBzd3SnaX7zJIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy M3nHUlI{OjV6N{Sw
LNCaP MYTD[YxtKF[rYXLpcIl1gSCDc4PhfS=> MnOzNE0yODByIH7N NXi0dYhqOC12IHS= NXrJdG9LemWmdXPl[EBNVkOjUDDj[YxtKH[rYXLpcIl1gSCrbjDhJIRwe2VvIHHu[EB1cW2nLXTldIVv\GWwdDDtZY5v\XMEoB?= MkmzNlMzPTh5NEC=
PC-3  NEDpdZRHfW6ldHnvckBCe3OjeR?= M3\ZWlExKM7:TR?= NGnXTVkyOiCq NYXwVo5ucW6mdXPld{BifXSxcHjh[5k> NFXqeIwzOzJ3OEe0NC=>

... Click to View More Cell Line Experimental Data

In vivo Oral dosing of AZD5363 (100, 300 mg/kg) to nude mice causes dose- and time-dependent reduction of PRAS40, GSK3β, and S6 phosphorylation in BT474c xenografts, reversible increases in blood glucose concentrations, and dose-dependent decreases in 2[18F]fluoro-2-deoxy-d-glucose (18F-FDG) uptake in U87-MG xenografts. Chronic oral dosing of AZD5363 (130, 200, and 300 mg/kg) causes dose-dependent growth inhibition of xenografts derived from various tumor types, including HER2+ breast cancer models that are resistant to trastuzumab. AZD5363 also significantly enhances the antitumor activity of docetaxel, lapatinib, and trastuzumab in breast cancer xenografts. [2]

Protocol

Kinase Assay
+ Expand

Caliper Off-Chip Incubation Mobility Shift assay:

The ability of AZD5363 and other compounds to inhibit the activity of AKT1, AKT2, and AKT3 is evaluated by the Caliper Off-Chip Incubation Mobility Shift assay. Active recombinant AKT1, AKT2, or AKT3 are incubated with a 5-FAM-labeled custom-synthesized peptide substrate together with increasing concentrations of inhibitor. Final reactions contained 1 to 3 nM AKT1, AKT2, or AKT3 enzymes; 1.5 mM peptide substrate; ATP at K m for each AKT isoform; 10 mM MgCl2, 4 mM DTT, 100 mM HEPES, and 0.015% Brij-35. The reactions are incubated at room temperature for 1 hour and stopped by the addition of buffer containing 100 mM HEPES, 0.015% Brij-35 solution, 0.1% coating reagent, 40 mM EDTA, and 5% DMSO. Plates are then analyzed using a Caliper LC3000, allowing for separation of peptide substrate and phosphorylated product by electrophoresis with subsequent detection and quantification of laser induced fluorescence.
Cell Research
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  • Cell lines: 182 solid and hematologic tumor cell lines
  • Concentrations: ~30 μM
  • Incubation Time: 72 hours
  • Method: Cell proliferation assay is determined by 2 methods, MTS and Sytox Green. Briefly, cells are seeded in 96-well plates and incubated overnight at 37 ℃, 5% CO2. Cells are then exposed to concentrations of AZD5363 ranging from 30 to 0.003μM for 72 hours. For the MTS endpoint, cell proliferation is measured by the CellTiter AQueous Non-Radioactive Cell Proliferation Assay reagent in accordance with the manufacturer's protocol. For the Sytox Green endpoint, Sytox Green nucleic acid dye diluted in TBS-EDTA buffer is added to cells (final concentration of 0.13 μM) and the number of dead cells detected using an Acumen Explorer. Cells are then permeabilized by the addition of saponin (0.03% final concentration, diluted in TBS-EDTA buffer), incubated overnight and a total cell count measured. Predose measurements are made for both MTS and Sytox Green endpoints, and concentration needed to reduce the growth of treated cells to half that of untreated cells values are determined using absorbance readings (MTS) or live cell counts.
    (Only for Reference)
Animal Research
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  • Animal Models: Female nude mice and male SCID mice with BT474c, U87MG, KPL-4, HCC-1187 xenografts.
  • Formulation: In 10% DMSO 25% w/v Kleptose HPB
  • Dosages: 130 mg/Kg - 300 mg/Kg
  • Administration: p.o.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 86 mg/mL (200.5 mM)
Water <1 mg/mL
Ethanol <1 mg/mL

* 1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 428.92
Formula

C21H25ClN6O2

CAS No. 1143532-39-1
Storage powder
in solvent
Synonyms N/A

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02576444 Recruiting Cancer Yale University|Dana-Farber Cancer Institute|Vanderbilt-Ingram Cancer Center November 2015 Phase 2
NCT02465060 Recruiting Advanced Malignant Neoplasm|Lymphoma|Refractory Malignant Neoplasm|Solid Neoplasm National Cancer Institute (NCI) August 2015 Phase 2
NCT02664935 Recruiting Non-Small Cell Lung Cancer|Carcinoma, Squamous Cell|Adenocarcinoma University of Birmingham|Cancer Research UK|AstraZeneca|Pfizer|Experimental Cancer Medicine Centre Network March 2015 Phase 2
NCT02449655 Recruiting Advanced Gastric Adenocarcinoma Samsung Medical Center February 2015 Phase 2
NCT02451956 Recruiting Advanced Gastric Cancer Samsung Medical Center January 2015 Phase 2
NCT02525068 Recruiting Adenocarcinoma of the Prostate Institute of Cancer Research, United Kingdom|Royal Marsden NHS Foundation Trust December 2014 Phase 2

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID