AZD5363

Catalog No.S8019

AZD5363 Chemical Structure

Molecular Weight(MW): 428.92

AZD5363 potently inhibits all isoforms of Akt(Akt1/Akt2/Akt3) with IC50 of 3 nM/8 nM/8 nM in cell-free assays, similar to P70S6K/PKA and lower activity towards ROCK1/2. Phase 2.

Size Price Stock Quantity  
In DMSO USD 235 In stock
USD 147 In stock
USD 470 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

4 Customer Reviews

  • G-H. Analysis of cells treated with AZD5363 (500 nM) for 24 hrs. G.Western analysis of whole cell lysates. H. Cells labeled with Annexin V-FITC for 6h were imaged. Individual points are average for replicates assessed in duplicate. Midlines are the average ± S.D. Student’s T-test.

    Cancer Res, 2016, 76(16):4752-64.. AZD5363 purchased from Selleck.

    (A) 239 cells stably expressing Flag-IL-17RA (293-IL-17RA cell line) were treated with 2 μM AZD5363 (a pan-Akt inhibitor) and/or 50 ng/ml insulin for the indicated time periods; Western blot analysis was performed for the indicated proteins; exogenous, IL-17RA transfected into 293 cells; endogenous, endogenous IL-17RA expressed in 293 cells.

    Oncotarget, 2016, 7(12):13651-66. AZD5363 purchased from Selleck.

  • Inhibition of PI3K and Akt by LY294002 and AZD5363 was confirmed by analyzing expression and activation status of the ribosomal protein S6 by Western blot analysis of PC-3 clone #14 cells treated with 0.5 or 5 uM AZD5363 (AZD) and 10 uM LY294002 (LY). Tubulin was used as a loading control.

    Cell Commun Signal 2014 12(1), 61. AZD5363 purchased from Selleck.

    LNCaP, LNCaP95, VCaP and 22Rv1 cells were transfected with control or pooled siRNA for AKT 1–3 isoforms for 36 hours. Cells were then further treated with 5 uM AZD5363 (B) for another 18 hours. Protein lysates were immunoblotted with AR (N-20), AR-V7, Pan-AKT, p-AKT(ser473) and β-Actin antibodies. Results were repeated in more than three independent experiments.

    PLoS One 2014 9(10), e108780. AZD5363 purchased from Selleck.

Purity & Quality Control

Choose Selective Akt Inhibitors

Biological Activity

Description AZD5363 potently inhibits all isoforms of Akt(Akt1/Akt2/Akt3) with IC50 of 3 nM/8 nM/8 nM in cell-free assays, similar to P70S6K/PKA and lower activity towards ROCK1/2. Phase 2.
Features Moderate preclinical tolerability, and PD characteristics of an AKT inhibitor. Distinct profile from other AKT inhibitors in clinical development.
Targets
Akt1 [1]
(Cell-free assay)
Akt2 [1]
(Cell-free assay)
Akt3 [1]
(Cell-free assay)
ROCK2 [1]
(Cell-free assay)
3 nM 8 nM 8 nM 56 nM
In vitro

AZD5363 is a potent Akt inhibitor with IC50 of 3 nM, 8 nM and 8 nM for Akt1, Akt2 and Akt3, respectively. [1] AZD5363 inhibits phosphorylation of AKT substrates in cells with a potency of approximately 0.3 to 0.8 μM. AZD5363 inhibits the proliferation of 41 of 182 solid and hematologic tumor cell lines with a potency of < 3 μM. [2] Activating mutations in PIK3CA, loss or inactivation of tumor suppressor PTEN, or HER2 amplification all are significantly predictive of responsiveness to AZD5363. Additionally, correlation is also seen between the RAS mutation status of cell lines and resistance to AZD5363. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MCF7 Mke1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MY[yNFAhdk1? MUi2JIQ> NVHiXpNqcW6lcnXhd4VlKGS{dXegd4Vve2m2aY\peJkhd2ZiND3PTHQh[W6mIH\1cJZme3S{YX70 M{TaVlI3OzVzM{Kz
ZR75 M4jLTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mmf2NVAxKG6P M1T4OFYh\A>? MknUbY5kemWjc3XkJIRzfWdic3Xud4l1cX[rdImgc4YhPC2RSGSgZY5lKG[3bI\ld5Rz[W62 M3zuTVI3OzVzM{Kz
T74D Ml;0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MU[xNFAhdk1? NEDsOW03KGR? M{PQfolv[3KnYYPl[EBlenWpIIPlcpNqfGm4aYT5JI9nKDRvT1jUJIFv\CCodXz2[ZN1emGwdB?= MofWNlY{PTF|MkO=
1%MCF7 MnywS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmnrOFAxKG6P NV7VT|VkPiCm M2TXbolv[3KnYYPl[EBlenWpIIPlcpNqfGm4aYT5JI9nKDRvT1jUJIFv\CCodXz2[ZN1emGwdB?= MkDpNlY{PTF|MkO=
MCF7 LTED NUW1OXE3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV23XoRrOjByIH7N NEHmZY03KGR? MoG4bY5kemWjc3XkJIRzfWdic3Xud4l1cX[rdImgc4YhPC2RSGSgZY5lKG[3bI\ld5Rz[W62 NHjrd5IzPjN3MUOyNy=>
ZR75 LTED MorkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmC3NVAxKG6P MnXVOkBl M2C1T4lv[3KnYYPl[EBlenWpIIPlcpNqfGm4aYT5JI9nKDRvT1jUJIFv\CCodXz2[ZN1emGwdB?= MmHvNlY{PTF|MkO=
T74D LTED NUHvVllzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2DMUVExOCCwTR?= MUG2JIQ> M4TMPIlv[3KnYYPl[EBlenWpIIPlcpNqfGm4aYT5JI9nKDRvT1jUJIFv\CCodXz2[ZN1emGwdB?= MVGyOlM2OTN{Mx?=
TamR NI\B[JpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mm\IOFAxKG6P M1;yO|Yh\A>? MnnCbY5kemWjc3XkJIRzfWdic3Xud4l1cX[rdImgc4YhPC2RSGSgZY5lKG[3bI\ld5Rz[W62 MmD3NlY{PTF|MkO=
HCC1954 M2PWcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NETTXlAxNTFwM{Wg{txO NXX0O5lYPSCm M1zuUoVvcGGwY3XzJJRp\SCpcn;3eIghcW6qaXLpeIlwdiCxZjDBXmQ5QTNz NFvmVnMzPjB7NUS3OS=>
BT474c MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3HERlAuOS5|NTFOwG0> NG\KfYE2KGR? Mnm3[Y5p[W6lZYOgeIhmKGe{b4f0bEBqdmirYnn0bY9vKG:oIFHaSFg6OzF? NG\0[VUzPjB7NUS3OS=>
KPL4 NEey[HlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NG\BSpoxNTFwM{Wg{txO M2\GWlUh\A>? M{C1e4VvcGGwY3XzJJRp\SCpcn;3eIghcW6qaXLpeIlwdiCxZjDBXmQ5QTNz MnzFNlYxQTV2N{W=
SKBR3 MnLmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{\iblAuOS5|NTFOwG0> MXq1JIQ> NFrZNpFmdmijbnPld{B1cGViZ4Lve5RpKGmwaHnibZRqd25ib3[gRXpFQDl|MR?= NGDzTJUzPjB7NUS3OS=>
MR49C NEnwNWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M37aUFAuPSEQvF2= MXy0PEBp MU\pcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uh\GWyZX7k[Y51KG2jbn7ldi=> M{\IUFI2OTVzMEGy
MR49F Mk\lS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUKwMVUh|ryP Mn7VOFghcA>? Mn\5bY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmKGSncHXu[IVvfCCvYX7u[ZI> NF7kVZMzPTF3MUCxNi=>
NCI-H522 MoDpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlXPTWM2OD1zMT6zJEjDuTJwNzmg{txO NHLDPYIzPDl3N{[4Ni=>
PC-9 NVnzUodYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnrDTWM2OD17LkOgLOKyOS5{KTFOwG0> M2TjXFI1QTV5Nkiy
NCI-H522 MUnGeY5kfGmxbjDBd5NigQ>? NFnHbWgyNzVxMUCg{txO MWi0M|I1KGh? MluxbY5kemWjc3XzJGFMXCCyaH;zdIhwenmuYYTpc44> NWW5cYliOjR7NUe2PFI>
PC-9 M4\pNWZ2dmO2aX;uJGF{e2G7 Mo\XNU82NzFyIN88US=> MY[0M|I1KGh? NEm0VoVqdmO{ZXHz[ZMhSUuWIIDoc5NxcG:{eXzheIlwdg>? M2rLZ|I1QTV5Nkiy
HGC27 NVzvR5dLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX;JNJl4UUN3ME2wMlQ1PSEQvF2= M4\3PFI1ODh6M{iy
IM95m M3;sZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFLSS2xKSzVyPUCuOVEh|ryP NVS0UW5GOjRyOEizPFI>
AGS MkHyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{nDcGlEPTB;MD61OVIh|ryP MkDJNlQxQDh|OEK=
NCI-N87 M2fKTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUjB[3l6UUN3ME2xMlA{PyEQvF2= M3H2SlI1ODh6M{iy
23132/87 NEPYSXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVHreXdtUUN3ME2xMlY4OSEQvF2= NGjmXXYzPDB6OEO4Ni=>
MKN1 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVK5VYFTUUN3ME2yMlQzOSEQvF2= MXuyOFA5QDN6Mh?=
SNU-620 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{CwSGlEPTB;Mz6zPFQh|ryP M3[xPVI1ODh6M{iy
SNU-638 MkmwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYXvNHRvUUN3ME20MlUzOyEQvF2= MYmyOFA5QDN6Mh?=
SNU-1 NHjRdmFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MluxTWM2OD13LkK1PEDPxE1? NWHsXmpCOjRyOEizPFI>
SNU-601 NWDm[pB4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVfmc4JnUUN3ME21Mlk{QCEQvF2= NILQWJAzPDB6OEO4Ni=>
SNU-668 MonOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkO0TWM2OD14LkCwN{DPxE1? MnnzNlQxQDh|OEK=
HS746T M{XoS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUPJR|UxRTZwMEi0JO69VQ>? Mk\aNlQxQDh|OEK=
KATO III Ml3ZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX;JR|UxRTdwMk[3JO69VQ>? NHTyT3QzPDB6OEO4Ni=>
SNU-484 M2H2ZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1G0TmlEPTB;Nz6zPVIh|ryP MWSyOFA5QDN6Mh?=
SNU-16 NVf4OHZNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYmwem1LUUN3ME2xNU4xQTdizszN M2OwV|I1ODh6M{iy
OCUM-1 M3joWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mo[wTWM2OD1zND61NVUh|ryP NGXmdWMzPDB6OEO4Ni=>
NUGC-3 MnvKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmjyTWM2OD1{MT64O|Mh|ryP MlLaNlQxQDh|OEK=
AZ521 NGfORmpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWe2TmdPUUN3ME2yOU41PDhizszN NUPCeYE{OjRyOEizPFI>
SNU-216 NGLi[Y1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlTKTWM2OD1|MDFOwG0> NG\LN|gzPDB6OEO4Ni=>
NUGC-4 MnjmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4r6WmlEPTB;M{Cg{txO MVGyOFA5QDN6Mh?=
SNU-5 NGr2eXlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIHkOFNKSzVyPUOwJO69VQ>? NEXCWmUzPDB6OEO4Ni=>
GTL-16 M{\mUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWnJR|UxRTNyIN88US=> NHL2XG0zPDB6OEO4Ni=>
MKN74 Ml6yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MojUTWM2OD1|MDFOwG0> Mm[4NlQxQDh|OEK=
PAMC82 M1;GN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYLBWXltUUN3ME2zNEDPxE1? NY\OOFMzOjRyOEizPFI>
LNCaP NHm0PI9HfW6ldHnvckBCe3OjeR?= MXq1JO69VQ>? NEThSIQxNTJ2IHi= M2TyVIlv\HWlZYOgRWtVWzR5MzDhcoQhSUuWVEOwPEBxcG:|cHjvdplt[XSrb36gbY4h[SC2aX3lJIRmeGWwZHXueEBu[W6wZYK= M3;ySVI{QTZ4NkKx
C4-2  NX74U5dGTnWwY4Tpc44hSXO|YYm= MW[1JO69VQ>? Ml7KNE0zPCCq Mln1bY5lfWOnczDBT3RUPDd|IHHu[EBCU1SWM{C4JJBpd3OyaH;yfYxifGmxbjDpckBiKHSrbXWg[IVx\W6mZX70JI1idm6nch?= MXWyN|k3PjZ{MR?=
LNCaP M3\nW2Z2dmO2aX;uJGF{e2G7 Mlz3OUDPxE1? NIDDVJoxNTJ2IHi= MkXUbY5pcWKrdIOgdIhwe3Cqb4L5cIF1cW:wIH;mJJRp\SCmaYP0ZYwhSUuWLYDheIh4[XliYnnvcYFzc2W{czDpcoNtfWSrbnegVHJCWzRyLDDlTWY1TSxiNFWtRnAyNCCvVF;SMEBidmRiUEewJHM3KGurbnHz[UBqdiCjIITpcYUu\GWyZX7k[Y51KG2jbn7ldi=> Mn7jNlM6PjZ4MkG=
C4-2  MYrGeY5kfGmxbjDBd5NigQ>? NVvpSWpXPSEQvF2= NH\6bo8xNTJ2IHi= NHTJcGVqdmirYnn0d{BxcG:|cHjvdplt[XSrb36gc4YhfGinIHTpd5RidCCDS2StdIF1cHejeTDibY9u[XKtZYLzJIlv[2y3ZHnu[{BRWkGVNECsJIVKTjSHLDC0SU1DWDFuIH3UU3ItKGGwZDDQO|AhWzZia3nuZZNmKGmwIHGgeIlu\S2mZYDlcoRmdnRibXHucoVz M2nFZ|I{QTZ4NkKx
LNCaP M1nwVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV\Se4hwOS1zMECwNEBvVQ>? MUWwMVMh\A>? NH;6fYlqdmirYnn0d{Bk\WyuII\pZYJqdGm2eTDkc5NmKGSncHXu[IVvfGy7 M4jJUVI{QTZ4NkKx
C4-2  MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHzDZ4gyNTFyMECwJI5O MV:wMVMh\A>? M4q0RolvcGmkaYTzJINmdGxidnnhZoltcXS7IHTvd4Uh\GWyZX7k[Y51dHl? NHzxZnMzOzl4Nk[yNS=>
LNCaP NGjaVWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmXzNVAxNTVyMECgcm0> MU[3NkBp NULuZ4FFcW6lcnXhd4V{KHSqZTDmdoFkfGmxbjDv[kBk\WyuczD1coRmemexaX7nJINmdGxiZHXheIg> M{j4VVI{QTZ4NkKx
C4-2  MnSzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mlj0NVAxNTVyMECgcm0> NXj1TVhGPzJiaB?= NHvKNVZqdmO{ZXHz[ZMhfGinIH\yZYN1cW:wIH;mJINmdGy|IIXu[IVz\2:rbnegZ4VtdCCmZXH0bC=> M{Hhd|I{QTZ4NkKx
PC-3 NHvvOnpHfW6ldHnvckBCe3OjeR?= Mn63NE42NzFxMUCg{txO MVK0PEBp NV:0NWxS\G:5boLl[5Vt[XSnczD0bIUheGixc4Doc5J6dGG2aX;uJI9nKGSxd37zeJJm[W1icHH0bJdigSCycn;0[YlveyCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? NV3GZ4h1OjN{NUi3OFA>
DU145  M3zPe2Z2dmO2aX;uJGF{e2G7 MnjTNE42NzFxMUCg{txO NWL4V3FuPDhiaB?= Mn;s[I94dnKnZ4XsZZRmeyC2aHWgdIhwe3Cqb4L5cIF1cW:wIH;mJIRwf26|dILlZY0heGG2aIfhfUBxem:2ZXnud{BqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NFTMcoEzOzJ3OEe0NC=>
LNCaP Mn\WR4VtdCCYaXHibYxqfHliQYPzZZk> MlPtNE0yODByIH7N NFTySXgxNTRiZB?= M{DVdJJm\HWlZXSgUG5E[VBiY3XscEB3cWGkaXzpeJkhcW5iYTDkc5NmNSCjbnSgeIlu\S2mZYDlcoRmdnRibXHucoVzyqB? MYSyN|I2QDd2MB?=
PC-3  M4fx[WZ2dmO2aX;uJGF{e2G7 NGK1TG0yOCEQvF2= M33OPVEzKGh? NGXQZZNqdmS3Y3XzJIF2fG:yaHHnfS=> MlvJNlMzPTh5NEC=

... Click to View More Cell Line Experimental Data

In vivo Oral dosing of AZD5363 (100, 300 mg/kg) to nude mice causes dose- and time-dependent reduction of PRAS40, GSK3β, and S6 phosphorylation in BT474c xenografts, reversible increases in blood glucose concentrations, and dose-dependent decreases in 2[18F]fluoro-2-deoxy-d-glucose (18F-FDG) uptake in U87-MG xenografts. Chronic oral dosing of AZD5363 (130, 200, and 300 mg/kg) causes dose-dependent growth inhibition of xenografts derived from various tumor types, including HER2+ breast cancer models that are resistant to trastuzumab. AZD5363 also significantly enhances the antitumor activity of docetaxel, lapatinib, and trastuzumab in breast cancer xenografts. [2]

Protocol

Kinase Assay:[1]
+ Expand

Caliper Off-Chip Incubation Mobility Shift assay:

The ability of AZD5363 and other compounds to inhibit the activity of AKT1, AKT2, and AKT3 is evaluated by the Caliper Off-Chip Incubation Mobility Shift assay. Active recombinant AKT1, AKT2, or AKT3 are incubated with a 5-FAM-labeled custom-synthesized peptide substrate together with increasing concentrations of inhibitor. Final reactions contained 1 to 3 nM AKT1, AKT2, or AKT3 enzymes; 1.5 mM peptide substrate; ATP at K m for each AKT isoform; 10 mM MgCl2, 4 mM DTT, 100 mM HEPES, and 0.015% Brij-35. The reactions are incubated at room temperature for 1 hour and stopped by the addition of buffer containing 100 mM HEPES, 0.015% Brij-35 solution, 0.1% coating reagent, 40 mM EDTA, and 5% DMSO. Plates are then analyzed using a Caliper LC3000, allowing for separation of peptide substrate and phosphorylated product by electrophoresis with subsequent detection and quantification of laser induced fluorescence.
Cell Research: [2]
+ Expand
  • Cell lines: 182 solid and hematologic tumor cell lines
  • Concentrations: ~30 μM
  • Incubation Time: 72 hours
  • Method: Cell proliferation assay is determined by 2 methods, MTS and Sytox Green. Briefly, cells are seeded in 96-well plates and incubated overnight at 37 ℃, 5% CO2. Cells are then exposed to concentrations of AZD5363 ranging from 30 to 0.003μM for 72 hours. For the MTS endpoint, cell proliferation is measured by the CellTiter AQueous Non-Radioactive Cell Proliferation Assay reagent in accordance with the manufacturer's protocol. For the Sytox Green endpoint, Sytox Green nucleic acid dye diluted in TBS-EDTA buffer is added to cells (final concentration of 0.13 μM) and the number of dead cells detected using an Acumen Explorer. Cells are then permeabilized by the addition of saponin (0.03% final concentration, diluted in TBS-EDTA buffer), incubated overnight and a total cell count measured. Predose measurements are made for both MTS and Sytox Green endpoints, and concentration needed to reduce the growth of treated cells to half that of untreated cells values are determined using absorbance readings (MTS) or live cell counts.
    (Only for Reference)
Animal Research:[2]
+ Expand
  • Animal Models: Female nude mice and male SCID mice with BT474c, U87MG, KPL-4, HCC-1187 xenografts.
  • Formulation: In 10% DMSO 25% w/v Kleptose HPB
  • Dosages: 130 mg/Kg - 300 mg/Kg
  • Administration: p.o.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 86 mg/mL (200.5 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order:
1% CMC Na
For best results, use promptly after mixing.
30mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 428.92
Formula

C21H25ClN6O2

CAS No. 1143532-39-1
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02576444 Recruiting Cancer Joseph Paul Eder|Dana-Farber Cancer Institute|Vanderbilt-Ingram Cancer Center|Yale University November 2015 Phase 2
NCT02465060 Recruiting Advanced Malignant Neoplasm|Lymphoma|Recurrent Plasma Cell Myeloma|Recurrent Solid Neoplasm|Refractory Malignant Neoplasm|Refractory Plasma Cell Myeloma National Cancer Institute (NCI) August 2015 Phase 2
NCT02664935 Recruiting Non-Small Cell Lung Cancer|Carcinoma, Squamous Cell|Adenocarcinoma University of Birmingham|Cancer Research UK|AstraZeneca|Pfizer|Experimental Cancer Medicine Centre Network March 2015 Phase 2
NCT02451956 Recruiting Advanced Gastric Cancer Samsung Medical Center January 2015 Phase 2
NCT02525068 Recruiting Adenocarcinoma of the Prostate Institute of Cancer Research, United Kingdom|Royal Marsden NHS Foundation Trust December 2014 Phase 2
NCT02208375 Recruiting Breast Cancer|Malignant Female Reproductive System Neoplasm M.D. Anderson Cancer Center|AstraZeneca|National Cancer Institute (NCI) November 2014 Phase 1|Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Akt Signaling Pathway Map

Akt Inhibitors with Unique Features

Related Akt Products

Tags: buy AZD5363 | AZD5363 supplier | purchase AZD5363 | AZD5363 cost | AZD5363 manufacturer | order AZD5363 | AZD5363 distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID