MK-2206 2HCl

Catalog No.S1078

MK-2206 2HCl Chemical Structure

Molecular Weight(MW): 480.39

MK-2206 2HCl is a highly selective inhibitor of Akt1/2/3 with IC50 of 8 nM/12 nM/65 nM in cell-free assays, respectively; no inhibitory activities against 250 other protein kinases observed. Phase 2.

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Cited by 182 Publications

21 Customer Reviews

  • Inhibition of AKT signaling abolishes MKK4 phosphorylation on Ser78 in injured axons. Cultures of sensory neurons were treated with 5 µM MK-2206 or 5 µM GDC-0068 for 1 hr prior to axotomy. Axonal proteins harvested at indicated time points after axotomy were subjected to immunoblot analysis.

    Cell, 2015, 160(1-2): 161-76 . MK-2206 2HCl purchased from Selleck.

    j,k, Gene expression ofMYOCD (j) and ACTA2 ( SMA, k) after applying inhibitors of key components involved in the DDR2 downstream signalling pathway to HSCs cultured within 3D collagen matrix subjected to stretching (ST) (n=3, one-way ANOVA, **P=0.0036, ****P<0.0001). l,m, Expression of SMA was significantly reduced after treatment with related inhibitors in early-stage FμNs. (n=4, one-way ANOVA, ***P=0.001, ****P<0.0001). AKT-i: MK-2206

    Nature Materials, 2017, 16:1252-1261.. MK-2206 2HCl purchased from Selleck.

  • Cancer Cell 2013 24, 766-76. MK-2206 2HCl purchased from Selleck.

    The PI3K or AKT inhibition does not restore sensitivity to WZ4002 in PC9 WZR. PC9GR4 or WZR10 cells were treated with WZ4002 alone at the indicated concentrations or in combination with the AKT inhibitor MK-2206 (1 uM). MK-2206 effectively inhibited AKT in both cells.

    Cancer Discov 2012 2, 934-47. MK-2206 2HCl purchased from Selleck.

  • Rap1b negatively regulates neutrophil transcellular migration by limiting PI3K-Akt signaling. (A-D) Effect of Akt inhibitor MK2206 (2 uM), Src inhibitor PP2 (10 uM), or vehicle control (DMSO) on WT or Rap1b-/- neutrophil functions. (A) Percentage of neutrophil transendothelial migration in 3D migration model. (B) ECM degradation assessed on Oregon green-labeled gelatin matrix; (left) representative images on (bar, 10 um) and (right) bar graph is percentage of matrix degradation. (C) Percentage of cells forming multiple protrusions. (D) Percentage of neutrophils present at junction of activated bEND.3 in 3D migration assay. Mean ?SD; n = 3 independent experiments. **, P < 0.01; ***, P < 0.001; NS, not significant using unpaired Student's t test).

    J Exp Med 2014 211(9), 1741-58. MK-2206 2HCl purchased from Selleck.

    VE-cadherin-induced Akt activation mediates YAP phosphorylation and translocation in ECs. HUVECs were starved for 1h and treated with thrombin (1U) for 1h. Total cell lysates were probed with anti-pAkt, Akt or b-actin antibody. The representative blots of three independent experiments are depicted, and the normalized values for p-Akt are shown. HUVECs were cultured and starved as described as in d and incubated for 8h in complete medium with the Akt inhibitor, MK-2206 (1 uM). pAkt, Akt, pYAP and YAP were detected by western blotting using specific antibodies.

    Nat Commun 2015 6:6943. MK-2206 2HCl purchased from Selleck.

  • Inhibitors of AKT or ERK overcome SDF-1a-mediated resistance to ibrutinib-triggered PARP and caspase 3 cleavage in CXCR4S338X-expressing BCWM.1 cells. CXCR4S338X-expressing WM cells were treated with ibrutinib (0.5 uM) alone or in the presence of SDF-1a (20 nM) and/or the AKT inhibitors MK-2206 (0.5 uM) and AZD-5363 (0.5 uM); or the MEK inhibitors AS-703026 (0.25 uM), AZD-6244 (0.5 uM) and UO126 (5.0 uM). (a) Immunoblotting results for phosphoAKT (S473) and phospho-ERK (T202/Y204) in CXCR4S338X-expressing BCWM.1 cells pretreated with ibrutinib with and without AKT or ERK inhibitors, then subjected to SDF-1a stimulation for 2 min. The inhibitory effect of AZD-5363 on AKT, which is known to paradoxically hyper-phosphorylate pAKT(S473) was confirmed by inhibition of the phospho-activity for the downstream AKT targets glycogen synthase kinase 3b and pS6. (b) Immunoblotting results for cleaved PARP and cleaved caspase 3 in CXCR4S338X-expressing BCWM.1 cells treated with ibrutinib and/or AKT or ERK inhibitors for 6 h at IC50 doses. GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

    Leukemia 2015 29(1), 169-76. MK-2206 2HCl purchased from Selleck.

    Induction of apoptosis positively correlates with FOXO3a protein levels and phosphorylation. (a) Treatment of 11 cell lines seeded in 6-well plates with 8 μM MK-2206 or DMSO for 48 hours. Nuclei were harvested for PI staining and FACS analysis and the differences between drug and no drug are displayed as in Figure b. Both conditions were performed in triplo for each cell line. Below the FACS data is a we stern blot of cell lysates from this panel treated with 8 μM MK-2206 or DMSO and harvested at 24 hours. Each cell line name corresponds to the two western lanes below it and the four cell cycle bars above it.

    Cancer Res 2013 73, 2189-98. MK-2206 2HCl purchased from Selleck.

  • IC50 values for an Akt inhibitor (MK-2206) and a MEK inhibitor (U0126) in a panel of mouse and human anaplastic (ATC) and follicular (FTC) carcinoma cell lines. On the bottom, a Western blotting showing the effect on the activation of Akt and ERK1/2 of one hour exposure to these inhibitors (MK-2206: 500 nM, U0126: 10 μM).

    Oncotarget 2011 2, 1109-26. MK-2206 2HCl purchased from Selleck.

    Mean IL-8 concentrations determined by ELISA of the supernatants of HeLa cells infected with wild-type Salmonella. Kinase inhibitors are indicated on the x axis, and the target families of the inhibitors are indicated above each column. CEC, chelerythrine; Pim Inh, Pim-1 inhibitor 2. Inhibitors that significantly affected IL-8 production relative to the control (P < 0.05, Bonferroni post hoc test from one-way ANOVA) are indicated with an asterisk. Relative cell viability is also shown, as determined by reduction of XTT by viable cells. A450, absorbance at 450 nm.

    Sci Signal 2011 4, rs9. MK-2206 2HCl purchased from Selleck.

  • Reducing cellular levels of PtdIns5P by overexpression of PIP4Kα inhibits clonogenic growth of U2OS but enhances cell survival in response to H2O2 stimulation. A) U2OS cells (1000) were plated, allowed to attach overnight, and then treated with DMSO (control), PI-103 (0.5 μM), U0126 (5 μM), or SB203580 (5 μM) for 9 d. Cell colonies were stained using crystal violet. Dried plates were scanned. Representative results are shown. B) U2OS cells (1000) were plated, allowed to attach overnight, and then treated with DMSO (control), MK-2206 (1 μM), triciribine (1 μM), KU0063794 (1 μM), or PI-103 (0.5 μM) for 9 d. Cell colonies were stained using crystal violet. Dried plates were scanned. Representative results are shown.

    FASEB J 2013 27, 1644-56. MK-2206 2HCl purchased from Selleck.

    A. Western blot analysis of pAKT activation in ovarian cancer cell lines treated with exogenous IGF-1. B. Activation of pAKT by IGF-1 in low-grade ovarian cancer cell lines was blocked by the AKT inhibitor MK-2206 in a dose-dependent manner.

    Gynecol Oncol 2011 123, 13-8. MK-2206 2HCl purchased from Selleck.

  • Confocal microscopy images of NO formation. DAF 2 DA-loaded washed platelets (1.0109 platelets/mL) were preincubated at 378C with saline (A), and then stimulated for 1min with 0.1 (B), 1.0 (C) or 10 (D) μM AEA. In Panel (E-F-G) washed platelets were preincubated with 1 μM SR1 (E), 1 Mm MK2206 (F) or 20 μM LY294002 (G), and then stimulated for 1min with 1.0 μM AEA. In panel (H) is reported the effect of 5 mg/mL collagen, used as a positive control. All the experiments were carried out in the presence of 100 μM L-arginine. NO formation was visualized by confocal microscropy as detailed in Methods.

     

     

    J Cell Biochem 2011 112, 924–932. MK-2206 2HCl purchased from Selleck.

    The AEA effect on eNOS phosphorylation. Washed platelets (1.0109 platelets/mL), preincubated at 378C with saline, 1 μM SR1, 1 μM SR2, 20 μM LY294002, 1 μM MK2206, 1.0 μM EGTA, or 30 μM BAPTA/AM, were stimulated for 1 min with 1.0 μM AEA. At the end of incubation suitable aliquots were immunoblotted with anti-p-eNOSser1177 as detailed in Methods.Blots are representative of five independent experiments.

     

     

    J Cell Biochem 2011 112, 924–932. MK-2206 2HCl purchased from Selleck.

  • Effect of selected agents on NOx and cGMP formation induced by AEA. Washed platelets (1.0109 platelets/mL), prewarmed at 378C with saline or 1mM SR1, 1 μM SR2, 20 μM URB597 (URB), 1 μM MK2206 (MK) or 20 μM LY294002 (LY), were incubated for 1min with 100 μM L-arginine in the presence of 1.0 μM AEA. NOx (panel A) and cGMP (panel B) content were determined as detailed in Methods. Each bar represents the meanSD of five independent experiments carried out in triplicate. Student,s t-test: P <0.0001 versus none;P <0.0005; P <0.005 versus AEA

     

     

    J Cell Biochem 2011 112, 924–932. MK-2206 2HCl purchased from Selleck.

    (B) Dose-response curves for DIPG 4 and SF7761 cells treated with the small molecule AKT inhibitor MK-2206 (error bars represent SEM).

    Oncol Rep, 2018, 39(2):455-464. MK-2206 2HCl purchased from Selleck.

  • C.U87MG cells were treated with vehicle or 1 μM MK-2206 for 1 hr, and then irradiated with 6 Gy. Total cell lysate was harvested 1 hr after IR and subjected to Western blot analysis with the indicated antibody. Cells without IR treatment were used as a control.

    D. Cells were treated with vehicle (control) or 1 μM MK-2206 for 1 hr, then irradiated with indicated dosage. 4 hr after IR, cells were fed with drug-free medium, and incubated for another 20 hr at 37°C, after which they were trypsinized and seeded for clonogenic survival assay. Colony-forming efficiency was determined 14 d later.
     

     

    Radiat Oncol 2009 4, 43. MK-2206 2HCl purchased from Selleck.

    MK-2206 2HCL inhibited the sorafenib-induced PI3K/mTOR pathway activation and enhanced the cytotoxic effects of sorafenib in resistant cell lines. (A) HUH-7 hepatoma cells treated with sorafenib (5 μM) for 24 h with or without pretreatment with specific kinase inhibitors (MK-2206 2HCL, 15 μM). The expressions of p-AKT and cleaved PARP were revealed by Western blotting. (B) SK-HEP-1 hepatoma cells treated with sorafenib (5 μM) for 24 h with or without pretreatment with specific kinase inhibitors (MK-2206 2HCL, 15 μM). The expressions of p-AKT and cleaved RARP were revealed by Western blotting. (C) HUH-7 hepatoma cells treated with sorafenib (5 μM) with or without the kinase inhibitors for 24 h. The proportions of apoptotic cells were evaluated by annexin V labeling. (D) SK-HEP-1 hepatoma cells treated with sorafenib (5 μM) with or without the kinase inhibitors for 24 h. The proportions of apoptotic cells were evaluated by annexin V labeling. (*P < 0.05, HUH-7, SK-HEP-1 are control groups, R-HUH-7, R-SK-HEP-1 are resistant groups).

    J Surg Res, 2016, 206(2):371-379. MK-2206 2HCl purchased from Selleck.

  • Comparative effects of inhibitors by immunofluorescence microscopy study. Confluent HC11 cells were grown on poly-L-lysine-coated glass coverslips (immunofluorescence) and on plastic plates (biochemical control) and then treated with inhibitors according to the standard procedure. Upper part: the biochemical action of the inhibitors was tested to validate the immunofluorescence results. Cellular proteins were analyzed by SDS-PAGE and the immunoblots were successively probed with anti-ADRP, anti-β-casein, and anti- β-actin antibodies and their respective HRP-conjugated secondary antibodies. Each experimental condition was performed in duplicate. Lower part: cells were fixed, permeabilized and subjected to immunofluorescence microscopy using antiserum against ADRP and TRITC-conjugated secondary antibody (red).

    Biochim Biophys Acta 2012 1823, 987-96. MK-2206 2HCl purchased from Selleck.

    After starved in serum-free medium for 24h, Breast cancer cells incubated with the indicated concentrations of MK-2206 for 3h,followed by 15-minute stimolation of 100ng/ml EGF.

     
     

     

    Dr. Zhang of Tianjin Medical University. MK-2206 2HCl purchased from Selleck.

  •  

    PI3K pathway signaling in GDC-0941 and MK-2206 treated MCF-7 derivatives with PIK3CA or  AKT1 mutations. Cells were grown in medium containing 5% FBS and treated with vehicle or increasing concentrations of GDC-0941 (0 nM, 50 nM, 100 nM, and 400 nM) or MK-2206 (0 nM, 100 nM, 250 nM, 1000 nM). After 24 hours of drug treatment, lysates were prepared and equal amounts of protein were load ed onto SDS-PAGE gels and blotted with the indicated antibodies.

    MK-2206 2HCl purchased from Selleck.

Purity & Quality Control

Choose Selective Akt Inhibitors

Biological Activity

Description MK-2206 2HCl is a highly selective inhibitor of Akt1/2/3 with IC50 of 8 nM/12 nM/65 nM in cell-free assays, respectively; no inhibitory activities against 250 other protein kinases observed. Phase 2.
Features The first allosteric small molecule inhibitor of Akt to enter clinical development.
Targets
Akt1 [1]
(Cell-free assay)
Akt2 [1]
(Cell-free assay)
Akt3 [1]
(Cell-free assay)
8 nM 12 nM 65 nM
In vitro

MK-2206 is an allosteric inhibitor and is activated by the pleckstrin homology domain. MK-2206 inhibits auto-phosphorylation of both Akt T308 and S473. MK-2206 also prevents Akt-mediated phosphorylation of downstream signaling molecules, including TSC2, PRAS40 and ribosomal S6 proteins. [1] MK-2206 inhibits Ras wild-type (WT) cell lines (A431, HCC827, and NCI-H292) more potently when compared to Ras-mutant cell lines (NCI-H358, NCI-H23, NCI-H1299, and Calu-6). MK-2206 also shows synergistic responses in combination with cytotoxic agents such as erlotinib or lapatinib in lung NCI-H460 or ovarian A2780 tumor cells. [2] MK-2206 or siRNA-mediated Akt inhibition strongly activates autophagy in human glioma cells. However, eukaryotic elongation factor-2 (eEF-2) silencing suppresses MK-2206-induced-autophagy, with a promotion of apoptotic cell death. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
NCI-H292 NVy1fFQ5S3m2b4TvfIlkKEG|c3H5 MWSzJO69VQ>? M3fSTFczKGh? M4TqR2ROW09? M17JfmlvcGmkaYTpc44hd2ZiY3XscEBxem:uaX\ldoF1cW:w M1zXNFIxPTdzME[5
A431 MkDST4lv[XOnIFHzd4F6 NXjWbZlGPSEQvF2= NXvsZmo3PSCq MkXmSG1UVw>? MmLZV5VxeHKnc4Pld{B1cGVic3nncoFtcW6pIH;mJGFsfCCjbnSgSZJs MUCyNFU4OTB4OR?=
HepG2 MWPDfZRwfG:6aXOgRZN{[Xl? MlfMNVAh|ryP MmTYNlQhcA>? MWjEUXNQ M1uxOXNmdnOrdHn6[ZMhemW|aYP0ZY51KGOnbHzzJJRwKHSqZTDjfZRwfG:6aXOg[YZn\WO2IH;mJJNwemGoZX7pZi=> MX:yNVIxPTl{NR?=
Sk-Hep1 M1zPU2N6fG:2b4jpZ{BCe3OjeR?= NF;E[G0yOCEQvF2= NHnURlczPCCq M4juNWROW09? NW\Oc3k5W2Wwc3n0bZpmeyC{ZYPpd5RidnRiY3XscJMhfG9idHjlJIN6fG:2b4jpZ{Bm\m[nY4Sgc4Yhe2:{YX\lcolj NYPvZmM4OjF{MEW5NlU>
OCUT1 cells harbored PIK3CA (H1047R+/+) NYPk[mVtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHPPXoc{KM7:TR?= M4nvfFUh\A>? MVTEUXNQ MUTJR|UxRTBwMUSg{txO NETkNIUzOTJ6OUK2Oy=>
K1 cells harbored PIK3CA (E542K+/+) M3:zfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3;6WFMh|ryP MkmzOUBl NXK1UY5HTE2VTx?= MVjJR|UxRTBwNUKg{txO M4fCOVIyOjh7Mk[3
FTC133 cells harbored PTEN (allele deletion and R130+) NUDpUWFLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{LSd|Mh|ryP M17HPVUh\A>? M163fmROW09? NWj2cmNHUUN3ME2wMlE5KM7:TR?= M3LK[lIyOjh7Mk[3
C643 cells harbored HRAS (G13R+/−) MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4jFN|Mh|ryP NYW1cYJDPSCm NEfIZ3BFVVOR MYLJR|UxRTBwMkeg{txO M1ixeVIyOjh7Mk[3
Hth7 cells harbored NRAS (Q61R+/−) M{fQZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFe0W3M{KM7:TR?= MWW1JIQ> M4Hqd2ROW09? NXrLeVFSUUN3ME20MlUh|ryP MVOyNVI5QTJ4Nx?=
TPC1 cells harbored RET/PTC1 rearrangement MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mo[0N{DPxE1? M3LLcVUh\A>? NYi2TXNbTE2VTx?= NH7DNlVKSzVyPUCuOVkh|ryP M1PuXVIyOjh7Mk[3
Hth74 M1y1V2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1LiVVMh|ryP NXe0OZVQPSCm M1ztU2ROW09? MWHJR|UxRTJwMUmg{txO MVeyNVI5QTJ4Nx?=
KAT18 MnXZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIqwb2c{KM7:TR?= NYO4NoN5PSCm M3LOemROW09? MUDJR|UxRTRwNkKg{txO MV2yNVI5QTJ4Nx?=
SW1736 NVXHS4VXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUmxNFAh|ryP NWHnbGlSPSCm MnTVSG1UVw>? NHLiZXRKSzVyPUS3MlU3KM7:TR?= MUmyNVI5QTJ4Nx?=
WRO MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYrjdpJrOTByMDFOwG0> NHvrc4Q2KGR? NW[xfVdzTE2VTx?= NF[zbmlKSzVyPkGwNFAh|ryP MoDZNlEzQDl{Nke=
TAD2 NYGwfHpXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVSxNFAxKM7:TR?= NVnvfphSPSCm MX7EUXNQ M4P2Z2lEPTB-MUCwNEDPxE1? NFqwOXkzOTJ6OUK2Oy=>
LN229 Mn3XRZBweHSxc3nzJGF{e2G7 MWOwMlUh|ryP MUS2NEBp MVfEUXNQ MX\BeYdu\W62czDhdI9xfG:pZX7pZ{Bm\m[nY4TzJI9nKGenZnn0bY5q[g>? NITIeYwzOjB3N{mxOC=>
T98G MofaRZBweHSxc3nzJGF{e2G7 MWSwMlUh|ryP NUm0UJZvPjBiaB?= MWPEUXNQ NYW5dFFCSXWpbXXueJMh[XCxcITv[4VvcWNiZX\m[YN1eyCxZjDn[YZqfGmwaXK= MYSyNlA2PzlzNB?=
HC11 M{nvR2Z2dmO2aX;uJGF{e2G7 MVKxNEDPxE1? MoCxNlQhcA>? M1j4cWROW09? M1S4fWlvcGmkaYTzJO6zNWOjc3XpckBidmRiQVTSVEB{gW62aHXzbZM> NI\mbGgzOjR{Nk[yNS=>
MOLT-4 NUfZS|ZiS3m2b4TvfIlkKEG|c3H5 M2qxTlExKM7:TR?= M3ztNlQ5KGh? NF\Bc2hFVVOR NF\jZopKSzVyPUGuO-KBkc7:TR?= MXuyNlYyPDJ2Mx?=
CEM-R MlG3R5l1d3SxeHnjJGF{e2G7 M4qxOlExKM7:TR?= M4jTbFQ5KGh? MnrISG1UVw>? Mn;LTWM2OD1|LkRihKnPxE1? MWSyNlYyPDJ2Mx?=
CEM-S NVPrcYN{S3m2b4TvfIlkKEG|c3H5 NVL5XmJtOTBizszN NHvYV2M1QCCq M4rzRWROW09? NIP2NoxKSzVyPUWuNgKBkc7:TR?= MVmyNlYyPDJ2Mx?=
MOLT-4 NEDpVWFHfW6ldHnvckBCe3OjeR?= NVPpPIVCOTBizszN M2fz[FI1KGh? M4XYNmROW09? NEjEV2xDdG:la4OgZ4VtdHNiaX6geIhmKEdyL1exJJBp[XOnIH;mJJRp\SClZXzsJIN6[2yn NHjSWnkzOjZzNEK0Ny=>
MOLT-4 MoXzSpVv[3Srb36gRZN{[Xl? NHnpTW816oDLzszN MnrhOEBp MXLEUXNQ MXvJcoNz\WG|ZYOgeIhmKGGvb4XueEBw\iClbHXheoVlKEyFM1GvRkwh[SC5ZXzsMYV{fGGkbHnzbIVlKGG3dH;wbIFogSCvYYLr[ZI> MXqyNlYyPDJ2Mx?=
CEM-R NFm5W4dHfW6ldHnvckBCe3OjeR?= Mnu0OQKBkc7:TR?= NH3kV4g1KGh? NXnGRZo{TE2VTx?= NYLkNnE{UW6lcnXhd4V{KHSqZTDhcY92dnRib3[gZ4xm[X[nZDDMR|RCN0JuIHGge4VtdC2nc4ThZoxqe2inZDDheZRweGijZ4mgcYFzc2W{ M{jEXVIzPjF2MkSz
CEM-S M{W2S2Z2dmO2aX;uJGF{e2G7 M4rSRlTjiIoQvF2= NWXW[2dkPCCq NX\hdmxDTE2VTx?= NFPDco1KdmO{ZXHz[ZMhfGinIHHtc5VvfCCxZjDjcIVifmWmIFzDOWEwSixiYTD3[YxtNWW|dHHicIl{cGWmIHH1eI9xcGGpeTDtZZJs\XJ? M2fnWVIzPjF2MkSz
HepG2 cell Mk\jT4lv[XOnIFHzd4F6 NVnnbZlTOjBizszN MUeyOEBp M{e5VGROW09? MVLEc5dvemWpdXzheIV{KHSqZTDwbI9{eGixconsZZRqd25ib3[gRWtV NYCyZog2OjN5OUezNVk>
HepG2 cell NEnIOY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV6zNEDPxE1? NV7HWYJwOjRiaB?= MWjEUXNQ NFG1dodKdmirYnn0d{Bk\WyuIHfyc5d1cA>? Mlz2NlM4QTd|MUm=
HepG2 cell NWLFdpl1SXCxcITvd4l{KEG|c3H5 MkX2NlAh|ryP NHHwN3kzPCCq M1T2dGROW09? M4LEVGlv\HWlZYOgZ4VtdCCjcH;weI9{cXN? NFzHPIkzOzd7N{OxPS=>
GEO MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUC1NFAhdk1? MVu3NkBp NWX1T|FVTE2VTx?= MUDJcohq[mm2czDj[YxtKGe{b4f0bC=> NFTvXXczPDV6MUKzNS=>
CNE-1 M1zuc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIj2O24yOCEQvF2= MWG5OkBp NVfoSnRZTE2VTx?= MlnNTWM2OD1{Lkm2JO69VQ>? MUOyOVM{Pjl{NR?=
CNE-2 M360fGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV7UfHI6OTBizszN NHXzUow6PiCq NGPxVYxFVVOR MoL6TWM2OD12LkWzJO69VQ>? MnTINlU{OzZ7MkW=
HONE-1 M4DLfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NID3RYUyOCEQvF2= M{LieVk3KGh? Mk\kSG1UVw>? NXTxUIdnUUN3ME2zMlM4KM7:TR?= NWHsNnV[OjV|M{[5NlU>
SUNE-1 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXz1W5M2OTBizszN M1OwNVk3KGh? M3TSemROW09? M1jQZmlEPTB;MD61NkDPxE1? MXKyOVM{Pjl{NR?=
CNE-2 M2K2ZWZ2dmO2aX;uJGF{e2G7 NFzEUIYyOCEQvF2= NWfPTHlSPDhiaB?= MkezSG1UVw>? MoDLTY5lfWOnczDj[YxtKGO7Y3zlJIFzemW|dDDheEBIOQ>? NGjQUY8zPTN|NkmyOS=>
HONE-1 NYTTdXVwTnWwY4Tpc44hSXO|YYm= NHvvfHYyOCEQvF2= NGXNPXQ1QCCq MWLEUXNQ M{DKbGlv\HWlZYOgZ4VtdCCleXPs[UBienKnc4SgZZQhTzF? NGG5RZczPTN|NkmyOS=>
NEC8 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUL0ZoVCUUN3ME2wMlA6PjVzIN88US=> MUjTRW5ITVJ?
P12-ICHIKAWA MlXXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWTJR|UxRTBwMUG2NkDPxE1? M2\NdHNCVkeHUh?=
MDA-MB-175-VII NH7rS|NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXH3VppzUUN3ME2wMlE{PzN6IN88US=> MVvTRW5ITVJ?
AsPC-1 M1K4bWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoHiTWM2OD1yLkKyNVIzKM7:TR?= M4r6WXNCVkeHUh?=
T47D MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVzJR|UxRTBwMkiyOUDPxE1? Mnq1V2FPT0WU
HH NFvpOHNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHK0VZpKSzVyPUCuN|AzQDNizszN M2nwSXNCVkeHUh?=
MOLT-16 MlSzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFXMcpNKSzVyPUCuN|A{OiEQvF2= MYLTRW5ITVJ?
ES5 MkTNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEDmeohKSzVyPUCuN|Q1PTVizszN M1fTRXNCVkeHUh?=
RS4-11 NITYT|NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYDQOJpDUUN3ME2wMlM1PjFizszN NWi2dpU1W0GQR1XS
KARPAS-45 M{fWWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIXPRlhKSzVyPUCuN|c{OjFizszN Mn;6V2FPT0WU
NCI-H720 NXf4U5RZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUntTGZVUUN3ME2wMlM4Pjd7IN88US=> MlzYV2FPT0WU
H9 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYTMSWhwUUN3ME2wMlM5QDh|IN88US=> M{\QXXNCVkeHUh?=
EFM-19 M3;6d2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mnm3TWM2OD1yLkS0NFEh|ryP NWLZbHJOW0GQR1XS
SBC-1 M13JUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4D6dWlEPTB;MD60OFA{PSEQvF2= MYnTRW5ITVJ?
A4-Fuk NYjk[2hMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVjicopLUUN3ME2wMlQ3QDZ6IN88US=> MnzjV2FPT0WU
NCI-H1563 NGmycIRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1;GTWlEPTB;MD60PFE5QSEQvF2= NVL6VWo4W0GQR1XS
HCC1419 NUP2XllTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXXJR|UxRTBwNEi4PVIh|ryP M3vueHNCVkeHUh?=
H-EMC-SS M1nLUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV3YRnBnUUN3ME2wMlQ6QTN7IN88US=> NWe3W3hvW0GQR1XS
BHT-101 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NW\5T|N2UUN3ME2wMlUzQTZzIN88US=> MYLTRW5ITVJ?
IGROV-1 M2X5SGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NU\yb4k3UUN3ME2wMlU2OjR7IN88US=> MVvTRW5ITVJ?
HGC-27 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkDMTWM2OD1yLkW2O|g{KM7:TR?= M1zPT3NCVkeHUh?=
MDA-MB-361 MlvHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIjyToRKSzVyPUCuOVc4PjFizszN NUWzO2lwW0GQR1XS
KE-37 NXvDfIp1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoHvTWM2OD1yLkW4NlYh|ryP M1LPWnNCVkeHUh?=
HCC70 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGr0eW9KSzVyPUCuOVk5OjdizszN MXfTRW5ITVJ?
LNCaP-Clone-FGC M1PYZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmTWTWM2OD1yLk[xNFQ5KM7:TR?= NF3GSVNUSU6JRWK=
HAL-01 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnLZTWM2OD1yLk[yNVMh|ryP NFz2SoVUSU6JRWK=
HT NF\FSIxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlXUTWM2OD1yLk[zNlM6KM7:TR?= NIOxSmFUSU6JRWK=
MDA-MB-415 MnTjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVPXd2dLUUN3ME2wMlY{PjJ4IN88US=> MYDTRW5ITVJ?
NOS-1 M1vyPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnvyTWM2OD1yLk[zO|I{KM7:TR?= NYLIeFl6W0GQR1XS
DU-145 NWjDW5hKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEnIVJhKSzVyPUCuOlQ4PDVizszN NFHPU3ZUSU6JRWK=
OCUB-M NV7rOWJqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlTHTWM2OD1yLkewPVY3KM7:TR?= NHjub|NUSU6JRWK=
VA-ES-BJ MmS0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEjJe|VKSzVyPUCuO|MxOjVizszN M4ixOXNCVkeHUh?=
J-RT3-T3-5 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFf4fnpKSzVyPUCuO|Q1ODNizszN NUnH[48xW0GQR1XS
MOLT-4 M1\yfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4fXO2lEPTB;MD64NFU5OiEQvF2= NY\wO|AxW0GQR1XS
NB7 MkDSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4fVdWlEPTB;MD64NlQyOSEQvF2= NXPCTVJoW0GQR1XS
L-363 NEjHSm5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2DhRmlEPTB;MD64N|Q1OiEQvF2= M1n3eXNCVkeHUh?=
NKM-1 NWrnWW5ZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1Gzc2lEPTB;MD64OlI2OyEQvF2= NEHwVlJUSU6JRWK=
HOP-92 NH75UY9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{Li[GlEPTB;MD64O|IzOyEQvF2= MlPZV2FPT0WU
OAW-42 NEOy[XNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXfJR|UxRTBwOEi3NkDPxE1? NGTpO3hUSU6JRWK=
HuO9 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{X4T2lEPTB;MD65Nlc2OSEQvF2= NVfzfIVVW0GQR1XS
MFE-280 NGHRXI1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHy1doRKSzVyPUCuPVY1PjVizszN M2LONHNCVkeHUh?=
EM-2 M12wSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXKwVJZPUUN3ME2wMlk4QTN7IN88US=> M2i4VXNCVkeHUh?=
NCI-H520 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVzJR|UxRTBwOUi1PVIh|ryP NUL1[W1mW0GQR1XS
LB2241-RCC NUixSFlKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{P6TGlEPTB;MD65PVc{PCEQvF2= MUnTRW5ITVJ?
SK-NEP-1 MlvVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHjEZZBKSzVyPUGuNVQ1QDVizszN MYjTRW5ITVJ?
LXF-289 NV;hVlQ4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUHmZWVFUUN3ME2xMlE4OTV4IN88US=> MmntV2FPT0WU
EPLC-272H NYXHWIk2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkT1TWM2OD1zLkG3NlU3KM7:TR?= M37BVnNCVkeHUh?=
COLO-684 NYjubItKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWjJR|UxRTFwMkO3NlUh|ryP NHu2c3JUSU6JRWK=
ES1 NF30Wo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{f3NmlEPTB;MT6yOFA3PSEQvF2= NUXIbVZCW0GQR1XS
DOHH-2 MkfoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MonPTWM2OD1zLkK4NlA{KM7:TR?= NVLPOYc3W0GQR1XS
CTB-1 MnzES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH6xV3ZKSzVyPUGuNlg6QSEQvF2= NYD5fIREW0GQR1XS
G-401 NGX2W5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX\JR|UxRTFwMkm3PVUh|ryP Ml\RV2FPT0WU
LoVo MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXrJR|UxRTFwM{K1N|Qh|ryP M{PmTXNCVkeHUh?=
Ramos-2G6-4C10 NEOxSGhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVvJR|UxRTFwM{O3NFEh|ryP MX3TRW5ITVJ?
MFM-223 MonlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXS1SFAyUUN3ME2xMlM1PDZzIN88US=> MojDV2FPT0WU
PA-1 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4XxV2lEPTB;MT6zOVI3PSEQvF2= NGDCcnBUSU6JRWK=
697 M2q0XWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX;zUohSUUN3ME2xMlM4PjF4IN88US=> NE\QNnRUSU6JRWK=
QIMR-WIL NGDESGZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFzGV5lKSzVyPUGuOFkyOTZizszN Mly5V2FPT0WU
HOS MmnxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn3yTWM2OD1zLkS5OVU5KM7:TR?= NGfh[JpUSU6JRWK=
DMS-273 M2XJTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYLsPGhzUUN3ME2xMlUyQTV7IN88US=> MlLSV2FPT0WU
ME-180 MnjwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFLBeWJKSzVyPUGuOVY5QTFizszN MVfTRW5ITVJ?
HCC2218 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX;JR|UxRTFwNkiyNlUh|ryP NFfZOIVUSU6JRWK=
CAL-54 NGn2XopIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVvJR|UxRTFwN{GyOFIh|ryP NUjUO4JTW0GQR1XS
OMC-1 NYC3XHBYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkLJTWM2OD1zLke0Olc4KM7:TR?= M33HU3NCVkeHUh?=
COR-L105 NY\QSYdqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUXJR|UxRTFwN{m3N|ch|ryP MYrTRW5ITVJ?
BV-173 MoDNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmLrTWM2OD1zLkixNFc1KM7:TR?= M37lVHNCVkeHUh?=
RKO M1O2Zmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWDYO4VsUUN3ME2xMlg4OTBzIN88US=> M1PVb3NCVkeHUh?=
SNU-387 NHLMVmhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlvFTWM2OD1zLki4OFA3KM7:TR?= M4C0WnNCVkeHUh?=
SW1088 NX7OOnZyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEX0blNKSzVyPUGuPVQ3ODZizszN NXyzRpdsW0GQR1XS
Hs-578-T M3PQTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4DPUmlEPTB;Mj6xNVQ{OyEQvF2= MUXTRW5ITVJ?
OC-314 MlrjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MW\JR|UxRTJwMUWwPFYh|ryP MV7TRW5ITVJ?
RMG-I M2\kZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlGyTWM2OD1{LkG2N|k5KM7:TR?= NGP5TJlUSU6JRWK=
NCI-H1395 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml71TWM2OD1{LkG4NFkyKM7:TR?= M1v0b3NCVkeHUh?=
GAMG MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXjJR|UxRTJwMkO4OFUh|ryP NFq1SG1USU6JRWK=
LB1047-RCC MoLiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml;4TWM2OD1{LkK0N|E4KM7:TR?= MoHqV2FPT0WU
MN-60 MlKyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1HvVmlEPTB;Mj6yPVkzOyEQvF2= M4rnPXNCVkeHUh?=
OAW-28 NXLQTHJKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWH0Umk3UUN3ME2yMlI6QTVzIN88US=> NIL6TppUSU6JRWK=
NCI-H2228 NHH5Z|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NG\4PHRKSzVyPUKuN|E2PTJizszN MUDTRW5ITVJ?
ABC-1 NVLpenJbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2DrW2lEPTB;Mj6zN|I2OyEQvF2= M3rMd3NCVkeHUh?=
LS-513 M{jDZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFvXRVdKSzVyPUKuN|M1QDRizszN MUnTRW5ITVJ?
KS-1 NX;oUHE4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWP0NWFUUUN3ME2yMlM5OTlzIN88US=> M3nacHNCVkeHUh?=
NB69 NE\tR4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHHtUXdKSzVyPUKuN|g6QDNizszN NIK0OFVUSU6JRWK=
VM-CUB-1 M1Szemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmnxTWM2OD1{LkO5NFg{KM7:TR?= M{nkc3NCVkeHUh?=
D-423MG MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXzJR|UxRTJwNEGwOFQh|ryP MVrTRW5ITVJ?
EW-18 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2\S[mlEPTB;Mj60NVk{QSEQvF2= NUO5SW86W0GQR1XS
YH-13 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MW\JR|UxRTJwNE[xOVMh|ryP NYTw[oFRW0GQR1XS
T-24 MnfHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVfJR|UxRTJwNEe4PFEh|ryP M3jlW3NCVkeHUh?=
ES8 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXnoUm9GUUN3ME2yMlQ6Ojh5IN88US=> MnjvV2FPT0WU
ES3 NVfHW5VRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml7uTWM2OD1{LkS5O|U6KM7:TR?= NFT1d4RUSU6JRWK=
RXF393 NUXU[VM{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF7lc4ZKSzVyPUKuOlA1QDdizszN M{PHRXNCVkeHUh?=
RPMI-8226 NFT4ZndIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlXQTWM2OD1{Lk[yPVU{KM7:TR?= NWO4Z2JxW0GQR1XS
AGS MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEHFcpJKSzVyPUKuO|IyOzdizszN MlTHV2FPT0WU
HCC1395 Mkf3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXzJR|UxRTJwN{WxPFch|ryP MmDLV2FPT0WU
MV-4-11 Mor6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUjJR|UxRTJwN{WyOlYh|ryP M33yTXNCVkeHUh?=
A204 MlnBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NG\wUZVKSzVyPUKuPFM5PzJizszN MmH6V2FPT0WU
MCF7 MljJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVfafnRLUUN3ME2yMlg3OTF5IN88US=> MUHTRW5ITVJ?
SNU-423 M2Hmdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnfRTWM2OD1{Lki5NlQzKM7:TR?= NWrvbGpzW0GQR1XS
NCI-H1048 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmPyTWM2OD1{Lkm2PFY2KM7:TR?= M4OwUXNCVkeHUh?=
GR-ST NWD1XohoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkPjTWM2OD1|LkC0OlEyKM7:TR?= NInKepZUSU6JRWK=
EoL-1- NUHwWYNwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWTJR|UxRTNwMEewOVgh|ryP NXiyU3VxW0GQR1XS
HuH-7 NWTCTVF{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGTDNGVKSzVyPUOuNFk1PjRizszN MYLTRW5ITVJ?
OS-RC-2 MoT1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnLpTWM2OD1|LkGxNVkh|ryP MXPTRW5ITVJ?
EW-3 NUHJVlN6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4jBdWlEPTB;Mz6xPVUzQSEQvF2= NYT1U2xGW0GQR1XS
NCI-H747 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4S5RWlEPTB;Mz6yNFY6PCEQvF2= NEG2TmpUSU6JRWK=
EW-16 NFz3UnJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mmj0TWM2OD1|LkKxPFc6KM7:TR?= M2rDcXNCVkeHUh?=
DOK MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn2xTWM2OD1|LkKyPFU6KM7:TR?= MUfTRW5ITVJ?
HCC2157 NHjvU3JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVjJR|UxRTNwM{ixO|kh|ryP NXHEZ2hRW0GQR1XS
OVCAR-3 NIniR4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVzoZWI5UUN3ME2zMlQxPzh4IN88US=> NXzZWIFjW0GQR1XS
NCI-H1623 NVnWdIUxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX3JR|UxRTNwNEGyNlQh|ryP NWDrOFBXW0GQR1XS
H4 Mo\nS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NW[2S5BVUUN3ME2zMlQ2PjJ4IN88US=> NHzRdJNUSU6JRWK=
SW1710 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV7zdVVNUUN3ME2zMlQ3Pjd6IN88US=> Mlz4V2FPT0WU
RT-112 M3HnN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHLIW4hKSzVyPUOuOVI{QDhizszN NH;HVJlUSU6JRWK=
DMS-114 M1nlWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWLJR|UxRTNwNkKyO|gh|ryP M4LtZnNCVkeHUh?=
AN3-CA M3;3TWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NG\1XnBKSzVyPUOuOlI1PTZizszN NVTXVXV6W0GQR1XS
KNS-62 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUnwR4Z2UUN3ME2zMlY{OzN6IN88US=> M3LZOnNCVkeHUh?=
SJRH30 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MY\JR|UxRTNwNkmxNlIh|ryP MX\TRW5ITVJ?
G-402 Mn[yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MljuTWM2OD1|LkewO|EyKM7:TR?= M{PFdXNCVkeHUh?=
MHH-PREB-1 NGfmXlNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MU\JR|UxRTNwN{KwN|gh|ryP NUHiSldsW0GQR1XS
P30-OHK M1zINmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1TqVmlEPTB;Mz64NFk4PiEQvF2= NUOxe4hmW0GQR1XS
RVH-421 NUDNbWt2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NY\BOnc2UUN3ME2zMlgyPzh6IN88US=> NGnSTG9USU6JRWK=
LU-134-A NVXHSGVjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MonaTWM2OD1|Lki4OFI5KM7:TR?= NYfpXIFvW0GQR1XS
ECC10 M1LiV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{nxZ2lEPTB;Mz65N|YzOiEQvF2= Mk[yV2FPT0WU
TGW NGDjXJFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2\xSmlEPTB;ND6wNlMxPSEQvF2= NGrQWVdUSU6JRWK=
MLMA MmXtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1Lsb2lEPTB;ND6wNlk3PiEQvF2= Mn3QV2FPT0WU
SCC-25 NUfM[mY5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M322N2lEPTB;ND6wOlU3PiEQvF2= MYDTRW5ITVJ?
TYK-nu MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXXJR|UxRTRwMEm1N|Qh|ryP NFz1d2VUSU6JRWK=
LAMA-84 MnXES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnfrTWM2OD12LkG0NVkyKM7:TR?= MYPTRW5ITVJ?
Calu-3 NVHmNppVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHX5NWdKSzVyPUSuNlQ1OTZizszN NHnKdZBUSU6JRWK=
NCI-H460 MofrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M37qU2lEPTB;ND6yOlQ1OyEQvF2= M{i5eHNCVkeHUh?=
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EW-11 NFn4ZVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXmzV29ZUUN3ME20MlQyQDN6IN88US=> MVHTRW5ITVJ?
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KLE M3LKRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVfK[nljUUN3ME20MlcxOTl6IN88US=> MUTTRW5ITVJ?
CAL-39 M4XPRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmrYTWM2OD12LkeyNVQ3KM7:TR?= NUjiO2JyW0GQR1XS
TI-73 MoPkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUWxepY{UUN3ME20MlgxPjB7IN88US=> M4HvOnNCVkeHUh?=
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CAL-27 M{XJdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoLmTWM2OD13LkS2N|M6KM7:TR?= Ml2zV2FPT0WU
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D-263MG NGC4VmdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH21OZdKSzVyPU[uOFUyPjlizszN NXntfYF7W0GQR1XS
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D-566MG Mlu1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2rRcGlEPTB;OD6wOFQzQSEQvF2= MkHkV2FPT0WU
JVM-3 MmC0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYHJR|UxRThwMUWyOlgh|ryP M2DuOXNCVkeHUh?=
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MDA-MB-157 NVz4eGViT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXnhOVdqUUN3ME25MlkzQDd6IN88US=> MlfCV2FPT0WU
A101D NHXTd5hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXnJR|UxRTlwOUm5O|Qh|ryP NU\ySJljW0GQR1XS
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SF539 M3TjeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1XTXGlEPTB;MUCuPVA1OSEQvF2= MoHtV2FPT0WU
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DEL MmrpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYGyNnloUUN3ME2xNU41OjRizszN NYP0UphHW0GQR1XS
SW954 M4rXOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUPqV4IyUUN3ME2xNU41PjZ6IN88US=> NXPMXWVGW0GQR1XS
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A427 NF\MNZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWGxN|A3UUN3ME2xNU44ODl|IN88US=> NEjvdnRUSU6JRWK=
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RPMI-7951 NV7hN2RTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnjzTWM2OD1zMj6xPFU1KM7:TR?= MYDTRW5ITVJ?
RH-1 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVfPS2psUUN3ME2xNk4zOTh2IN88US=> MmT5V2FPT0WU
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CHP-212 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml\iTWM2OD1zMz65O|M6KM7:TR?= NHvn[WFUSU6JRWK=
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BPH-1 M3L2Tmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkHJTWM2OD1zND6xOlY1KM7:TR?= Ml3qV2FPT0WU
SW780 NX\INoJET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXjJR|UxRTF2LkWwNlUh|ryP NH7BNo1USU6JRWK=
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JEG-3 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{LIcWlEPTB;MUSuOlMzPiEQvF2= M2S0RXNCVkeHUh?=
CAL-120 M{\5XWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1v5N2lEPTB;MUSuO|AzPyEQvF2= MYDTRW5ITVJ?
NCI-H23 NEWxPJNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{\DNGlEPTB;MUSuO|k6PyEQvF2= NFy2bGxUSU6JRWK=
MS-1 M3fKWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn61TWM2OD1zND65OlEyKM7:TR?= M3vBUHNCVkeHUh?=
PC-14 NH7qZm9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF\QVYdKSzVyPUG0Mlk3PTRizszN NXrUO5AxW0GQR1XS
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CCRF-CEM NWO4UZB6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1vjZ2lEPTB;MkSzMlc6PSEQvF2= NXvUSFVsW0GQR1XS
SH-4 MnvxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2r4O2lEPTB;MkS2MlA6KM7:TR?= NVXrcFhyW0GQR1XS
LS-1034 NFS4fVlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXrJR|UxRTJ2Nj6yOlYh|ryP NGnHdIlUSU6JRWK=
NCI-H2347 NUTnd2NFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1fpWGlEPTB;MkS3MlcyOyEQvF2= M33McnNCVkeHUh?=
RPMI-8866 MnTJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4\5OWlEPTB;MkS5MlI4KM7:TR?= MYTTRW5ITVJ?
GAK M1vNWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlHITWM2OD1{NUOuNFAzKM7:TR?= MofuV2FPT0WU
NB6 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MULJR|UxRTJ5MD6xJO69VQ>? NYTaNYx3W0GQR1XS
COLO-680N MnvNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWOybFRGUUN3ME2yO|IvPTJ5IN88US=> NInvfZlUSU6JRWK=
RERF-LC-MS M2S0NGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4e5PGlEPTB;Mke2MlAxPyEQvF2= MX\TRW5ITVJ?
TGBC11TKB M1nNUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFfaXWdKSzVyPUK3PE4yPzhizszN M1;ocXNCVkeHUh?=
C8166 NWflbZpzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1:2[2lEPTB;Mke4MlUxPiEQvF2= M13sXHNCVkeHUh?=
HDLM-2 Mly3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWXJR|UxRTJ7ND60NFkh|ryP Ml;qV2FPT0WU
IGR-1 NEDHT25Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXPJR|UxRTJ7NT62OVkh|ryP MYDTRW5ITVJ?
FADU M4S3Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXfJR|UxRTJ7Nz61NUDPxE1? MV3TRW5ITVJ?
L-428 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYT1eZRNUUN3ME2yPVcvPjF4IN88US=> MVjTRW5ITVJ?
LU-65 NV24WoM4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUXJR|UxRTNyND6zNkDPxE1? NUnDOpByW0GQR1XS
HEL M4SxSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml\0TWM2OD1|MEmuPVg{KM7:TR?= NYnlNWRxW0GQR1XS
NCI-H810 M{jHN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mo\QTWM2OD1|MUCuOVch|ryP MXjTRW5ITVJ?
C3A M1r5Smdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVn0OFRyUUN3ME2zNVEvQDB{IN88US=> MYnTRW5ITVJ?
NCI-H630 NULGbFhOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkLZTWM2OD1|M{KuNlk1KM7:TR?= MWnTRW5ITVJ?
KP-N-YN NYrSfodqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4\sVWlEPTB;M{SxMlEzOyEQvF2= NFz5UpVUSU6JRWK=
MOLT-13 NFX2VmlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1qwOWlEPTB;M{SyMlMzPiEQvF2= MlPaV2FPT0WU
NCI-H1993 NULpUVFXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGXJNHVKSzVyPUO0Nk4{PjVizszN NYTmSpB2W0GQR1XS
BE-13 NHXONpVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVmwUFhHUUN3ME2zOFQvOTZ5IN88US=> MlPRV2FPT0WU
IST-SL1 MoXUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3v3VGlEPTB;M{S3MlQxOSEQvF2= MWDTRW5ITVJ?
TE-9 MlHvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXHQZYFkUUN3ME2zOlMvPTh7IN88US=> NVq2NZdNW0GQR1XS
LU-135 MkHtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHjHXJVKSzVyPUO2O{4xOzVizszN MXzTRW5ITVJ?
T84 NH;KSndIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnTiTWM2OD1|N{SuO|EzKM7:TR?= NV;tenhSW0GQR1XS
K-562 M3;rfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1\NW2lEPTB;M{izMlM3KM7:TR?= NETRTmNUSU6JRWK=
SBC-5 Mnf6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWjGclZ{UUN3ME2zPFYvQTh3IN88US=> M4HhT3NCVkeHUh?=
NB17 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVPJR|UxRTN7Mj61PVYh|ryP NXLwZ2FWW0GQR1XS
NCI-H2052 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXLJR|UxRTN7OD60O|Ih|ryP M3zscHNCVkeHUh?=
HCC38 NFi4VIpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVPuXVBtUUN3ME20NFEvPTl|IN88US=> M4fMd3NCVkeHUh?=
NCI-H69 M{f2fWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHvnW49KSzVyPUS0NU4xQDNizszN NW\TWFN[W0GQR1XS

... Click to View More Cell Line Experimental Data

In vivo MK-2206 shows 60% TGI and inhibits more than 70 % of phospho-Akt1/2 (T308 and S473) in A2780 ovarian cancer xenografts at a dose of 240 mg/kg. [1] MK-2206 exhibits significant antitumor activity in NCI-H292 xenograft in combination with erlotinib or lapatinib. [2]

Protocol

Kinase Assay:

[4]

+ Expand

Akt kinases assay:

Akt kinases are assayed by a GSK-derived biotinylated peptide substrate. The extent of peptide phosphorylation is determined by Homogeneous Time Resolved Fluorescence (HTRF) using a lanthanide chelate (Lance)-coupled monoclonal antibody specific for the phosphopeptide in combination with a streptavidin-linked allophycocyanin (SA-APC) fluorophore which will bind to the biotin moiety on the peptide. When the Lance and APC are in proximity, a non-radiative energy transfer takes place from the Lance to the APC, followed by emission of light from APC at 655 nm. Working Solution: 100X protease inhibitor cocktail (PIC): 1mg/mL benzamidine, 0.5 mg/mL pepstatin, 0.5 mg/mL leupeptin, 0.5 mg/mL aprotinin; 10X assay buffer: 500 mM HEPES, pH7.5, 1% PEG, 16.6 mM EDTA, 1 mM EGTA, 1% BSA, 20 mM 9-glycerol phosphate; Quench buffer 50 mM HEPES pH 7.3, 16.6 mM EDTA, 0.1% BSA, 0.1% Triton X-100, 0.17 nM labeled monoclonal antibody, 0.0067 mg/mL SA-APC; ATP/MgCl2 working solution: 1X Assay buffer, 1 mM DTT, 1X PIC, 5% glycerol, active Akt; Peptide working solution: 1X Assay buffer, 1 mM DTT, 1X PIC, 5% glycerol, 2 TM GSK biotinylated peptide. The reaction is assembled by adding 16 µL of ATP/MgCl2 working solution to the appropriate wells. MK-2206 or vehicle (1.0 µL) is added followed by 10 µL of peptide working solution. The reaction is started by adding 13 μL of the enzyme working solution and mixing. The reaction is allowed to proceed for 50 min and then stopped by the addition of 60 µL HTRF quench buffer. The stopped reactions are incubated at room temperature for at least 30 min and then read in the instrument.
Cell Research:

[2]

+ Expand
  • Cell lines: A431, HCC827, NCI-H292, NCI-H358, NCI-H23, NCI-H1299, Calu-6 and NCI-H460 cells
  • Concentrations: 0, 0.3, 1 and 3 μM
  • Incubation Time: 72 or 96 hours
  • Method:

    MK-2206 is dissolved in DMSO as a stock solution and diluted by culture media before use. Cells are seeded at a density of 2-3 × 103 in 96-well plates and incubated for 24 hours. Then MK-2206 (0, 0.3, 1 and 3 μM) is added to the cells. Cell proliferation is determined after 72 or 96 hours.


    (Only for Reference)
Animal Research:

[2]

+ Expand
  • Animal Models: SK-OV-3, NCI-H292, HCC70, PC-3, and NCI-H460 models in male CD1-nude mice
  • Formulation: Formulated in 30% Captisol
  • Dosages: 120 mg/kg
  • Administration: Orally administered
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 14 mg/mL (29.14 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order:
15% Captisol
For best results, use promptly after mixing.
17 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 480.39
Formula

C25H21N5O.2HCl

CAS No. 1032350-13-2
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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Definitions of molecular mass, molecular weight, molar mass and molar weight:

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Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01306045 Recruiting Carcinoma, Non-Small-Cell Lung|Carcinoma, Small Cell Lung|Carcinoma, Thymic National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) January 21, 2011 Phase 2
NCT01802320 Active, not recruiting Colon Mucinous Adenocarcinoma|Colon Signet Ring Cell Adenocarcinoma|Rectal Mucinous Adenocarcinoma|Rectal Signet Ring Cell Adenocarcinoma|Recurrent Colon Carcinoma|Recurrent Rectal Carcinoma|Stage IIIA Colon Cancer|Stage IIIA Rectal Cancer|Stage IIIB Colon Cancer|Stage IIIB Rectal Cancer|Stage IIIC Colon Cancer|Stage IIIC Rectal Cancer|Stage IVA Colon Cancer|Stage IVA Rectal Cancer|Stage IVB Colon Cancer|Stage IVB Rectal Cancer National Cancer Institute (NCI) March 2013 Phase 2
NCT01783171 Active, not recruiting Pancreatic Adenocarcinoma|Recurrent Pancreatic Carcinoma|Stage III Pancreatic Cancer|Stage IV Pancreatic Cancer|Unresectable Pancreatic Carcinoma National Cancer Institute (NCI) January 2013 Phase 1
NCT01776008 Active, not recruiting Estrogen Receptor Positive|HER2/Neu Negative|Recurrent Breast Carcinoma|Stage IIA Breast Cancer|Stage IIB Breast Cancer|Stage IIIA Breast Cancer|Stage IIIB Breast Cancer|Stage IIIC Breast Cancer National Cancer Institute (NCI) January 2013 Phase 2
NCT01859182 Withdrawn Adenocarcinoma of the Gallbladder|Adenocarcinoma With Squamous Metaplasia of the Gallbladder|Adult Primary Cholangiocellular Carcinoma|Advanced Adult Primary Liver Cancer|Cholangiocarcinoma of the Extrahepatic Bile Duct|Localized Unresectable Adult Primary Liver Cancer|Metastatic Extrahepatic Bile Duct Cancer|Recurrent Adult Primary Liver Cancer|Recurrent Extrahepatic Bile Duct Cancer|Stage II Gallbladder Cancer|Stage IIIA Gallbladder Cancer|Stage IIIB Gallbladder Cancer|Stage IVA Gallbladder Cancer|Stage IVB Gallbladder Cancer|Unresectable Extrahepatic Bile Duct Cancer National Cancer Institute (NCI) January 2013 Phase 2
NCT01705340 Terminated Adenocarcinoma of the Gastroesophageal Junction|HER2-positive Breast Cancer|Male Breast Cancer|Recurrent Breast Cancer|Recurrent Esophageal Cancer|Recurrent Gastric Cancer|Stage IIIC Breast Cancer|Stage IIIC Esophageal Cancer|Stage IIIC Gastric Cancer|Stage IV Breast Cancer|Stage IV Esophageal Cancer|Stage IV Gastric Cancer National Cancer Institute (NCI) September 2012 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    What is your recommendation for preparing the solution and how would you deliver it to the mice (i.p. or gavage)?

  • Answer:

    You can resuspend MK-2206 in 15% Captisol at up to 17mg/ml. It's a suspension and is for oral gavage use only.

  • Question 2:

    I would want to know if MK-2206 could cross the blood brain barrier?

  • Answer:

    MK-2206 can cross BBB based on the following reference: https://clinicaltrials.gov/ct2/show/NCT01249105.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID