Triciribine

Catalog No.S1117 Synonyms: NSC 154020, VD-0002, vqd-002

Triciribine Chemical Structure

Molecular Weight(MW): 320.3

Triciribine is a DNA synthesis inhibitor, also inhibits Akt in PC3 cell line and HIV-1 in CEM-SS, H9, H9IIIB, U1 cells with IC50 of 130 nM and 20 nM, respectively; does not inhibit PI3K/PDK1; 5000-fold less active in cells lacking adenosine kinase. Phase 1/2.

Size Price Stock Quantity  
In DMSO USD 150 In stock
USD 120 In stock
USD 170 In stock
USD 570 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

2 Customer Reviews

  • Effects of FAK A., PI3K B. or Akt C. inhibition in D-Hep2/AURKA cells on dormancy-related protein expression as analyzed by western blotting. P130 and E2F4 levels were increased, while P107 and Ki67 were decreased. Dormancy-related protein ratio in D-Hep2/AURKA cells treated with TAE226 D., Omipalisib E. or Triciribine F.

    Oncotarget, 2016, 7(30):48346-48359. Triciribine purchased from Selleck.

    Effect of triciribine on the migration of (A) FaDu and (B) Hep2 cells. The cells were treated with 5 µM triciribine for different periods of time. *P<0.05 vs. control. Hpf, high-power field.

    Oncol Lett, 2016, 11(3):1889-1894.. Triciribine purchased from Selleck.

Purity & Quality Control

Choose Selective Akt Inhibitors

Biological Activity

Description Triciribine is a DNA synthesis inhibitor, also inhibits Akt in PC3 cell line and HIV-1 in CEM-SS, H9, H9IIIB, U1 cells with IC50 of 130 nM and 20 nM, respectively; does not inhibit PI3K/PDK1; 5000-fold less active in cells lacking adenosine kinase. Phase 1/2.
Targets
HIV-1 [2]
(CEM-SS, H9, H9IIIB, U1 cells)
Akt [1]
(PC3 cells)
20 nM 130 nM
In vitro

Triciribine exhibits maximum growth inhibition around 1-10 μM and inhibits phosphorylation of Akt, as well as downstream p70S6K, to basal levels at 100μM (IC50 = 130 nM). Triciribine shows particular promise for inhibiting growth in Nf1 and Trp53 mutant astrocytoma cells in a grade-dependent manner. The WHO II K1861-10 line is inhibited, incompletely (69% maximum inhibition), with a GI50 value of 1.7 μM for Triciribine, whereas higher-grade tumor lines (KR158, KR130, and SF295) are inhibited to a greater extent (>80% maximum inhibition) at lower GI50 values (0.4–1.1 mM). Importantly, Triciribine is much less effective at inhibiting primary astrocytes (GI5013.6 mM), suggesting that this inhibitor may show specificity for tumor cells. [1] Triciribine inihibits HIV-1with an IC50 of 20 nM. Greater than 90% inhibition is achieved at 0.1μM and complete inhibition of syncytia formation is achieved at 5μM. Associated cell toxicity in the same cell line for Triciribine is 46 μM, resulting in selectivity indices of 2250. Triciribine markedly inhibits HIV-1-induced p24 core antigen production, reverse transcriptase, and infectious virus production in a dose-dependent manner using HIV-1 acutedly infected CEM-SS, H9, and persistently infected H9III B and U1 cells. [2] Triciribine inhibits Akt phosphorylation at Thr308 and Ser473 and Akt activity in the human prostate cancer cell line PC-3. Triciribine sensitizes PC-3 cells to TRAIL- and anti-CD95-induced apoptosis, whereas the cells remain resistant to DNA damaging chemotherapeutics. [3] Triciribine is highly selective for Akt and does not inhibit the activation of phosphatidylinositol 3-kinase, phosphoinositide-dependent kinase-1, protein kinase C, serum and glucocorticoid-inducible kinase, protein kinase A, signal transducer and activators of transcription 3, extracellular signal-regulated kinase-1/2, or c-Jun NH2-terminal kinase. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human ACH2 cells NXHSNWtsS3m2b4TvfIlkyqCjc4PhfS=> NI\iRWZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCS0h{IHPlcIx{KGmwZnXjeIVlKHerdHigcIF1\W62IFj1cYFvKGmvbYXuc4Rm\mmlaXXuZ5khfmm{dYOgNUBjgSCPVGOgZZN{[XluIFPDOVA:OC5yMTFOwG0> MYGyNFA5PjF2OR?=
L1210 leukemic cells NGLUV|ZEgXSxdH;4bYPDqGG|c3H5 NXO5fFJMXGW|dHXkJIlvKH[rdILvJIZweiCleYTveI95cWOrdImgZYdicW6|dDDteZJqdmViTEGyNVAhdGW3a3XtbYMh[2WubIOsJGlEPTB;MD6wN|Uh|ryP NH7ubYoyODh6MkO3NS=>
human H9 cells M1XlWWZ2dmO2aX;uJIF{e2G7 MkC1RY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTJVu[W5iaX3teY5w\GWoaXPp[Y5kgSC4aYL1d{AyKFONMTDpcoZm[3SnZDDpckBpfW2jbjDIPUBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oII\pdoFtKFKndnXyd4UhfHKjboPjdolxfGG|ZTDifUBcO0ifVGTQJIlv[2:{cH;yZZRqd25iYYPzZZktKEmFNUC9NE4xOzZizszN M2PFS|IxODh4MUS5
human MCF7 cells MU\DfZRwfG:6aXRCpIF{e2G7 M3q3T2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMhcW5icILld4Vv[2Vib3[gNlAhfU1iY3jsc5JweXWrbnWgZpkhW1KEIHHzd4F6NCCJSUWwQVAvODVizszN Ml3PNVg3QTF6OUS=
AA5 cells M2\u[WZ2dmO2aX;uJIF{e2G7 MVPBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDv[kBJfW2jbjDpcY12dm:mZX\pZ4lmdmO7II\pdpV{KDFiM1KgbY5n\WO2ZXSgbY4hSUF3IHPlcIx{KGmwZnXjeIVlKHerdHigN{4yOyC3TDDv[kB3cXK3czDzeI9kcyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcnHsJHJmfmW{c3WgeJJidnOlcnnweIF{\SCkeTDbN2heXFSSIHnuZ49zeG:{YYTpc44h[XO|YYmsJGlEPTB;MD6xO{DPxE1? MkD1NlAxQDZzNEm=
human U1 cells NX\YfYtbTnWwY4Tpc44h[XO|YYm= M2fHd2FvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGh2dWGwIHntcZVvd2SnZnnjbYVv[3lidnnyeZMhOSCrbn\lZ5Rm\CCrbjDoeY1idiCXMTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcnHsJHJmfmW{c3WgeJJidnOlcnnweIF{\SCkeTDbN2heXFSSIHnuZ49zeG:{YYTpc44h[XO|YYmsJGlEPTB;Mj6zJO69VQ>? NImxbYczODB6NkG0PS=>
human MDM cells NUXxb|VHS3m2b4TvfIlkyqCjc4PhfS=> MWDDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSG0h[2WubIOgbY5n\WO2ZXSge4l1cCCKdX3hckBqdW23bn;k[YZq[2mnbnP5JJZqenW|IEGgRmFNKGK7IF3UV{Bie3OjeTygWGM2OD1yLk[2JO69VQ>? MVyyNFA5PjF2OR?=
human MDA-MB-231 cells M330PGN6fG:2b4jpZ:Kh[XO|YYm= MUHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHnuJJBz\XOnbnPlJI9nKDJyIIXNJINpdG:{b4H1bY5mKGK7IGPSRkBie3OjeTygS2k2OD1yLk[5JO69VQ>? M{TUUFE5PjlzOEm0
Huh-7 cells MV;GeY5kfGmxbjDhd5NigQ>? MnfvR49ueG:3bnSge4F{KHSnc4Tl[EBnd3JiaYTzJIFjcWyrdImgeI8hcW6qaXLpeEBp\XCjdHn0bZMhSyC4aYLhcEBTVkFicnXwcIlk[XSrb36gbY4hUHWqLUegZ4VtdHNiKHj1cYFvKGincHH0c41iKGOnbHzzLUwhTUN3ME2yJO69VQ>? MnTmNVUyPzd2NkS=
HFF cells NGnPdoZHfW6ldHnvckBie3OjeR?= NVm5dFNXUEOPVjDwcIFyfWViYYPzZZkhf2G|IIDldoZwem2nZDD1d4lv\yCKRl[gZ4VtdHNiYX7kJIVn\mWldDD3ZZMh[2GuY4XsZZRm\CCjczDhJJBmemOnboTh[4Uhd2ZicnXkeYN1cW:wIHnuJI52dWKncjDv[kBxdGGzdXXzMEBKSzVyPUKuOUDPxE1? NHLvZ5EyODh6MkO3NS=>
human MDA-MB-468 cells MlLhR5l1d3SxeHnjxsBie3OjeR?= MV7DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvNE[4JINmdGy|IHnuJJBz\XOnbnPlJI9nKDJyIIXNJINpdG:{b4H1bY5mKGK7IGPSRkBie3OjeTygS2k2OD1zMD6yPUDPxE1? NF7idJIyQDZ7MUi5OC=>
human 184B5 cells NHPNXXhEgXSxdH;4bYPDqGG|c3H5 NWe3ZlYzS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hOTh2QkWgZ4VtdHNiYomgV3JDKGG|c3H5MEBIUTVyPUSwJO69VQ>? MlvLNVg3QTF6OUS=

... Click to View More Cell Line Experimental Data

In vivo 1 mg/kg/day i.p. treated Triciribine inhibits OVCAR3, OVCAR8 and PANC1 tumor growth, which overexpressing Akt, by 90%, 88% and 80% in nude mice, respectively. However, Triciribine has little effect on the growth of OVCAR5 and COLO357 cells. [4]

Protocol

Kinase Assay:[1]
+ Expand

Akt Phosphorylation Changes Assay:

Cells are grown to 80%–90% confluency and stimulated for 5–10 minutes with 1–10 ng/mL of epidermal growth factor or platelet derived growth factor (PDGF)–AA with or without 10–20 mM of U0126 or LY-294002. Protein lysates (5–20 μg) are separated by 12%–15% SDS PAGE and analyzed by Western blot for Akt, phosphorylated Akt (phospho-Ser 473), MAPK, and phosphorylated MAPK (p44/42 phospho-Thr202/Tyr204) antibodies (1:1000).
Cell Research:[2]
+ Expand
  • Cell lines: CEM-SS cells
  • Concentrations: 0-500 μM
  • Incubation Time: 48 hours
  • Method: Triciribine is evaluated for cytotoxicity by seeding CEM-SS cells at a density of 1 × 104 cells/well in growth medium, using a 96-well flat-bottom plate. Serial fivefold dilutions of Triciribine are prepared in growth medium and added to the wells as a second overlay. After a 48-hours incubation at 37 °C, the cells are pulse labeled with [3H]dThd (1 μCi per well, specific activity 20 Ci/mmol) for 6 hours and the cells are harvested to measure total DNA synthesis.
    (Only for Reference)
Animal Research:[4]
+ Expand
  • Animal Models: OVCAR3, OVCAR8, PANC1, OVCAR5 and COLO357 tumor cells are injected s.c. into 80week-old female nude mice.
  • Formulation: Triciribine is dissolved in 20% DMSO.
  • Dosages: 1 mg/kg/day
  • Administration: Triciribine is administrated through i.p. injection once a day.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 64 mg/mL (199.81 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents individually and in order:
1% DMSO+30% polyethylene glycol+1% Tween 80
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 320.3
Formula

C13H16N6O4

CAS No. 35943-35-2
Storage powder
Synonyms NSC 154020, VD-0002, vqd-002

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01690468 Recruiting Ovarian Cancer Prescient Therapeutics, Ltd. September 2014 Phase 1|Phase 2
NCT01697293 Recruiting Breast Adenocarcinoma|Estrogen Receptor Positive|HER2/Neu Negative|Recurrent Breast Carcinoma|Stage IIB Breast Cancer|Stage IIIA Breast Cancer|Stage IIIB Breast Cancer|Stage IIIC Breast Cancer|Stage IV Breast Cancer Albert Einstein College of Medicine of Yeshiva University|National Cancer Institute (NCI) January 2012 Phase 1|Phase 2
NCT00642031 Completed Hematologic Malignancies|Leukemia Prescient Therapeutics, Ltd.|VioQuest Pharmaceuticals August 2006 Phase 1
NCT00363454 Completed Cancer Prescient Therapeutics, Ltd.|VioQuest Pharmaceuticals April 2006 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Related Antibodies

Akt Signaling Pathway Map

Akt Inhibitors with Unique Features

Related Akt Products

Tags: buy Triciribine | Triciribine supplier | purchase Triciribine | Triciribine cost | Triciribine manufacturer | order Triciribine | Triciribine distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID