Catalog No.S2805 Synonyms: HTS 466284
Molecular Weight(MW): 272.3
LY364947 is a potent ATP-competitive inhibitor of TGFβR-I with IC50 of 59 nM in a cell-free assay, shows 7-fold selectivity over TGFβR-II.
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The cytoplasmic and nuclear proteins were separated and the protein expression levels were determined by performing western blotting. LY364947 (1 uM), which is a potent ATP-competitive inhibitor of TGF-βRI, was used as the positive control. GAPDH and PARP were used as cytosolic and nuclear markers, respectively.
Chem Biol Interact 2014 217, 1-8. LY364947 purchased from Selleck.
Suppression of phosphorylated (p-)SMAD family member 2 (Smad2) signaling by LY364947 reverses the inductive effect of jumonji AT-rich interactive domain 1B (JARID1B) on the expression of glioma cancer stem cell-related markers. The expression of transforming growth factor-β1 (TGF-β1), p-Smad2, Smad2, CD133, octamer-binding transcription factor 4 (Oct4), nestin and BMI1 proto-oncogene, polycomb ring finger (Bmi-1) protein levels in LY364947-exposed U251-pBabe-JARID1B and its control cells were measured by western blot analysis. β-actin was used as the internal control for western blot analysis.
Int J Mol Med, 2016, 38(1):172-82. LY364947 purchased from Selleck.
Purity & Quality Control
Choose Selective TGF-beta/Smad Inhibitors
|Description||LY364947 is a potent ATP-competitive inhibitor of TGFβR-I with IC50 of 59 nM in a cell-free assay, shows 7-fold selectivity over TGFβR-II.|
LY364947 is an ATP competitive and tight-binding inhibitor, inhibiting phosphorylation of P-Smad3 by TGFβR-I kinase with Ki of 28 nM. LY364947 inhibits in vivo Smad2 phosphorylation within the NMuMg cells with IC50 of 135 nM. LY364947 reverses TGF-β-mediated growth inhibition in NMuMg cells with IC50 of 0.218 μM. LY364947 potentiates the xVent2-lux BMP4 response in NMuMg cells by 30% at concentrations as low as 0.25 μM. LY364947 (2 μM) prevents TGF-β-induced epithelial−mesenchymal transition in NMuMg cells.  LY364947 (3 μM) induces expression of Prox1 and LYVE-1 in almost all HDLECs after 24 hours.  LY364947 promotes nuclear export of Foxo3a, with low Smad2/3 and high Akt phosphorylation levels in leukaemia-initiating cells. LY364947 (< 20 μM) suppresses leukaemia-initiating cells colony-forming ability after co-culture with OP-9 stromal cells. 
|In vivo||LY364947 (1 mg/kg i.p.) accelerates lymphangiogenesis, as evidence by significantly increased the LYVE-1-positive areas, in a mouse model of chronic peritonitis. LY364947 (1 mg/kg i.p.) significantly increases the LYVE-1-positive areas in tumor tissues in tumor xenograft models using BxPC3 pancreatic adenocarcinoma cells.  LY364947 (25 mg /kg) increases p-Akt and decreases nuclear Foxo3a in leukaemia-initiating cells in CML-affected mice. |
Filter-binding assay:The IC50 of LY364947 at different enzyme concentrations are determined by the filter-binding assay. Typically, 40 μL reactions in 50 mM HEPES at pH 7.5, 1 mM NaF, 200 μM pKSmad3(−3), and 50 mM ATP containing a titration of each inhibitor with concentrations of 1600, 800, 400, 200, 100, 50, 25, and 0 nM are incubated at 30 °C for 30 min. The IC50 is calculated using a nonlinear regression method with GraphPad Prism software. The binding type is determined by plotting the correlation between enzyme concentrations and IC50 values.
-  Li HY, et al. J Med Chem, 2006, 49(6), 2138-2142.
-  Vogt J, et al. Cell Signal, 2011, 23(11), 1831-1842.
-  Peng SB, et al. Biochemistry, 2005, 44(7), 2293-2304.
|In vitro||DMSO||1 mg/mL (3.67 mM)|
|In vivo||Add solvents individually and in order:
4% DMSO+30% PEG 300+ddH2O
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