Home NF-κB NF-κB inhibitor JSH-23 For research use only.

JSH-23

JSH-23 is an inhibitor of NF-κB transcriptional activity with IC50 of 7.1 μM in RAW 264.7 cell line.

JSH-23 Chemical Structure

JSH-23 Chemical Structure

CAS: 749886-87-1

Selleck's JSH-23 has been cited by 142 publications

Purity & Quality Control

Batch: S735101 DMSO] 48 mg/mL] false] Ethanol] 20 mg/mL] false] Water] Insoluble] false Purity: 99.16%
99.16

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Signaling Pathway

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Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HK-2 cells Function assay 100 μM 3 h pretreatment with JSH-23 effectively blocked Ang II-induced nuclear p65 accumulation 30874544
U2OS/DR-GFP cells Function assay 10 and 20 µM JSH-23 treatment significantly decreased the HR frequency 30770924
MCF-7:5C Function assay 20 μmol/L 3 and 6 days JSH-23 completely blocked E2-induced apoptosis in MCF-7:5C cells 30224430
MCF-7:2A Function assay 20 μmol/L 3 and 6 days JSH-23 increased E2-induced apoptosis in MCF-7:2A cells 30224430
BMDM Function assay 60 μM 19-24 h JSH-23 (60 μM) induced a decrease of IL-1β release 30138321
HL60 cells Function assay 10 μM 48 h JSH-23 (10 μM) obviously decreased NF-κB DNA binding activity 30125548
BV2 cells Function assay 30 μM 1 h pretreatment of JSH-23 decreased the levels of IL-6 and NO production in LPS-stimulated microglia. 29890414
A549 cells Cell viability assay 5, 10, 20, 30, 40 and 50 μM 24 h with the increase in JSH-23 concentration, the inhibition rate for cell viability was gradually increased, and significant differences existed when the JSH-23 concentration was greater than 20 μM. 28281961
Click to View More Cell Line Experimental Data

Biological Activity

Description JSH-23 is an inhibitor of NF-κB transcriptional activity with IC50 of 7.1 μM in RAW 264.7 cell line.
Targets
NF-κB [1]
(RAW 264.7 cells)
7.1 μM
In vitro
In vitro JSH-23 inhibits LPS-induced nuclear translocation of NF-κB p65 without affecting IκBα degradation. JSH-23 inhibits LPS-induced apoptotic chromatin condensation, while does not show significant cytotoxic effects on the RAW 264.7 cells at <100 μM. [1] JSH-23 also decreases NO production and neuronal migration in LPS activated cultures primary cultures from developing mouse cerebellum. [2] Moreover, JSH-23 augments cisplatin cytotoxicity in ovarian cancer cells with CI values ranging from 0.35 to 0.85. [3]
Kinase Assay Measurement of NF-κB transcriptional activity
Macrophages RAW 264.7 transfected stably with reporter plasmid of pNF-κB-SEAP-NPT are treated with 1 μg/ml LPS and/or sample for 16 hours. As the reporter, SEAP activity in the cell-free culture media is measured as followed. Single cell-derived stable transfectants are plated in 5 ml of T-25 flask, and the media is decanted 24 h later. At this time, cells are washed twice with phosphate-buffered saline, and incubations are initiated by addition of new media. Chemicals are added to the culture medium after 24 h of incubations. Aliquots (25 ml) of medium from a control or chemical-treated cultures are taken at 0, 3, 20, 24, 48, and 72 h, heated at 65°C for 5 min to eliminate the alkaline phosphatase activity, and used immediately or stored at -20°C. Mixtures consisting of dilution buffer (25 ml), assay buffer (97 ml), culture media (25 ml), and 4-methylumbelliferyl phosphate (MUP, 1 mM, 3 ml) in each well of the 96-well plates are incubated for 60 min in the dark at room temperature. Fluorescence emits the product of the SEAP/MUP is measured at 449 nm using a 96-well plate fluorometer after excitation at 360 nm.
Cell Research Cell lines RAW 264.7 cells
Concentrations ~300 μM
Incubation Time 24 hours
Method Macrophages RAW 264.7 are incubated with various concentrations of JSH-23 compound for 24 h. The cells are treated with WST-1 solution and absorbance is measured at 450 nm.
Experimental Result Images Methods Biomarkers Images PMID
Western blot β1 Integrin / Fibronectin / p-Src / Src / α-SMA / NF-κB 25170871
ELSIA IL-6 / IL-23 28821374
Immunofluorescence Draq5 / p65 31072360
In Vivo
In vivo JSH-23 (3 mg/kg) significantly reverses the nerve conduction and nerve blood flow deficits by decreasing neuroinflammation and improving antioxidant defence in diabetic rats. [4]
Animal Research Animal Models STZ-induced diabetic rats
Dosages ~3 mg/kg
Administration Oral administration

Chemical Information & Solubility

Molecular Weight 240.34 Formula

C16H20N2
CAS No. 749886-87-1 SDF Download JSH-23 SDF
Smiles CC1=CC(=C(C=C1)NCCCC2=CC=CC=C2)N
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 48 mg/mL ( (199.71 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 20 mg/mL

Water : Insoluble


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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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