Caffeic Acid Phenethyl Ester

Catalog No.S7414 Synonyms: CAPE, Phenylethyl Caffeate

Caffeic Acid Phenethyl Ester Chemical Structure

Molecular Weight(MW): 284.31

Caffeic acid phenethyl ester is a potent and specific inhibitor of NF-κB activation, and also displays antioxidant, immunomodulatory and antiinflammatory activities.

Size Price Stock Quantity  
USD 97 In stock
USD 270 In stock

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

2 Customer Reviews

  • The protein expression alteration of p-NF-κB, NF-κB, PD-L1 in NP-69-LMP1 or NP-69 cell lines treated with 0, 0.5, and 1.0 μM Caffeic Acid Phenethyl Ester (CAPE) for 72 hours. β-actin was used to verify equal loading.

    Oncotarget, 2014, 5(23): 12189-202 . Caffeic Acid Phenethyl Ester purchased from Selleck.

    Representative apoptosis assay of LPS141 cells. Summary of apoptosis assay results. Data represent % apoptotic cells ± standard deviation (SD, error bars).

    Sci Rep, 2015, 5: 12580. Caffeic Acid Phenethyl Ester purchased from Selleck.

Purity & Quality Control

Choose Selective NF-κB Inhibitors

Biological Activity

Description Caffeic acid phenethyl ester is a potent and specific inhibitor of NF-κB activation, and also displays antioxidant, immunomodulatory and antiinflammatory activities.
Targets
NF-κB [1]
In vitro

Caffeic acid phenethyl ester blocks NF-κB activation induced by phorbol ester, ceramide, okadaic acid, and hydrogen peroxide by preventing the translocation of the p65 subunit of NF-κB to the nucleus. [1] In a series of tumor cell lines, Caffeic acid phenethyl ester shows promising antiproliferative activity with EC50 of 1.76, 3.16, 13.7, and 44.0 μM against murine colon 26-L5, murine B16-BL6 melanoma, human HT-1080 fibrosarcoma and human lung A549 adenocarcinoma cell lines, respectively. [2] Caffeic acid phenethyl ester, as a potent antioxidant, exerts its anti-apoptotic effect in cerebellar granule cells by blocking ROS formation and inhibiting caspase activity. [3] Moreover, Caffeic acid phenethyl ester attenuates the pro-inflammatory phenotype of LPS-stimulated HSCs, and LPS-induced sensitization of HSCs to fibrogenic cytokines by inhibiting NF-κB signaling. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse RAW264.7 cells NWTtcJVZTnWwY4Tpc44h[XO|YYm= MkTTNlQhcA>? NUHrfHU{UW6qaXLpeIlwdiCxZjDMVHMucW6mdXPl[EBvcXS{aXOgc5hq\GVicILv[JVkfGmxbjDpckBud3W|ZTDSRXczPjRwNzDj[YxteyCjc4Pld5Nm\CCjczDubZRzcXSnIHHjZ5VufWyjdHnvckBi\G2rbnnzeIVz\WRiMTDodkBj\W[xcnWgUHBUKGOqYXzs[Y5o\SCjbnSgcYVie3W{ZXSgZYZ1\XJiMkSgbJJ{KGK7IFfybYV{eyC{ZXHn[Y51KGG|c3H5MEBGSzVyPUCuNVk{KM7:TR?= M1HXWVE5PjZ5M{Kw
mouse 26-L5 cells NVzvWmV[WHKxbHnm[ZJifGmxbjDhd5NigQ>? NXzhU3R[PzJiaB?= M4LhZmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgcY92e2ViMk[tUFUh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygSWM2OD1yLkOg{txO NITIXWIyOjB{N{ezPS=>
human HeLa cells MknyVJJwdGmoZYLheIlwdiCjc4PhfS=> NXTyO45{PzJiaB?= MorTRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKZVzhJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFXDOVA:Oi5|NjFOwG0> NYTocmhVOTJyMke3N|k>
human Bewo cells MoLMSpVv[3Srb36gZZN{[Xl? MXLBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDoeY1idiCEZYfvJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiY3XscEBoem:5dHigZpkhVVSWIHHzd4F6NCCLQ{WwQVIvQTZizszN NXrOd25iOjVzNkC4N|c>
mouse J774.1 cells NIXo[2pHfW6ldHnvckBie3OjeR?= MWmyOEBp MlniTY5pcWKrdHnvckBw\iCOUGOtbY5lfWOnZDDOU{Bxem:mdXP0bY9vKGmwIH3veZNmKEp5N{SuNUBk\WyuczDh[pRmeiB{NDDodpMh[nliR4Lp[ZN{KHKnYXflcpQh[XO|YYmsJGlEPTB;ND64JO69VQ>? M3fsVFE2QTd2NkC4
human Bel7402 cells NGLScphEgXSxdH;4bYPDqGG|c3H5 NUfVU2pvS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSmWuN{SwNkBk\WyuczDifUBOXFRiYYPzZZktKEmFNUC9OU42KM7:TR?= MoroNVg6PTJ2MkC=
human LNCAP cells Ml;qSpVv[3Srb36gZZN{[Xl? M{jJWFI1KGh? M3nidGFvfGmjbnTyc4dmdmmlIHHjeIl3cXS7IHnuJIh2dWGwIFzOR2FRKGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDETHQue3SrbYXsZZRm\CCSU1GgdoVt\WG|ZTDh[pRmeiB{NDDodpMh[nliRVzJV2EtKEmFNUC9Ok4zKG6P MXSyOFA5ODFyNR?=
mouse BV2 cells NXzl[5E2TnWwY4Tpc44h[XO|YYm= NYXmTIpTOjRiaB?= MVHJcohq[mm2aX;uJI9nKG6rdILpZ{BwgGmmZTDwdo9lfWO2aX;uJIlvKEySUz3zeIlufWyjdHXkJI1wfXOnIFLWNkBk\WyuczD0doVifGWmIEOgbJJ{KGKnZn;y[UBNWFNiYXTkbZRqd25ibXXhd5Vz\WRiYX\0[ZIhOjRiaILzJIJ6KEe{aXXzd{Bie3OjeTygTWM2OD14LkSg{txO NXPiZo5TOjN6N{C3NFA>
mouse B16-BL6 cells NWjkUVhYWHKxbHnm[ZJifGmxbjDhd5NigQ>? MnLnO|IhcA>? M2Hpb2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgcY92e2ViQkG2MWJNPiClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBGSzVyPU[uO|kh|ryP M{LCW|EzODJ5N{O5
HUVEC cells MXjDfZRwfG:6aXRCpIF{e2G7 M3;HWVE5KGh? M4npRWN6fG:ycn;0[YN1cX[nIHHjeIl3cXS7IHHnZYlve3RiSELPNk1qdmS3Y3XkJI95cWSjdHn2[UB{fHKnc4OgbY4hUFWYRVOgZ4VtdHNiYX\0[ZIhOThiaILzJIJ6KEOnbHytWIl1\XJiQnz1[UBie3OjeR?= MUWyNFU6QDh7NB?=
human HT1080 cells MnjjVJJwdGmoZYLheIlwdiCjc4PhfS=> M{DOeFczKGh? NYexZ3duSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIWFExQDBiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhTUN3ME25MlUh|ryP MnmzNVIxOjd5M{m=
human HL60 cells NFzwd3lEgXSxdH;4bYPDqGG|c3H5 NYHsUWUxPzJiaB?= NH;he45EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJVDZyIHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9PU45KM7:TR?= NUDFOHR1OjB4N{O3Nlc>
human LS 174T cells NVHPSnZIS3m2b4TvfIlkyqCjc4PhfS=> M1;qZlczKGh? NHHlVI9EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBNWyBzN{TUJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOlA:QS57IN88US=> M2TIOlIxPjd|N{K3
human SGC7901 cells Mlv4SpVv[3Srb36gZZN{[Xl? MojtRY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU1fDO|kxOSClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJINmdGxiZ4Lve5RpKGK7IF3UWEBie3OjeTygTWM2OD1zND6yOkDPxE1? NHHHNmEzPTF4MEizOy=>
human Bel7404 cells Mo\TR5l1d3SxeHnjxsBie3OjeR?= MXW3NkBp NHWyWWVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBD\Wx5NEC0JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OTRwNTFOwG0> NWrSUnRrOjB4N{O3Nlc>
human BGC823 cells NUDCcVhGS3m2b4TvfIlkyqCjc4PhfS=> MXW3NkBp MWLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDCS2M5OjNiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2xPU4{KM7:TR?= NIDBTXEzODZ5M{eyOy=>
human HO8910 cells M2\yXWN6fG:2b4jpZ:Kh[XO|YYm= NIDZem04OiCq MVLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIU|g6OTBiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2yOU42KM7:TR?= M2GwVFIxPjd|N{K3
human MCF7 cells NFjnWGVEgXSxdH;4bYPDqGG|c3H5 MUHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIJ6KE2WVDDhd5NigSxiSVO1NF0zPi55IN88US=> MYKxPFk2OjR{MB?=
human A549 cells M4DwWWN6fG:2b4jpZ:Kh[XO|YYm= MmDBO|IhcA>? Moe2R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUK2Mlgh|ryP MWqyNFY4Ozd{Nx?=
human LNCAP cells NED4c4REgXSxdH;4bYPDqGG|c3H5 NIW1S4czPCCq MoL0R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUG5ESVBiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDJ2IHjyd{BjgSCZU2StNUBie3OjeTygTWM2OD1|Mz63JO69VQ>? NHzQSXAzPDB6MEGwOS=>
human HeLa cells M{HpPGZ2dmO2aX;uJIF{e2G7 MUPBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKZVzhJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiY3XscEBoem:5dHigZpkhVVSWIHHzd4F6NCCLQ{WwQVM4Njl6IN88US=> MoHGNlUyPjB6M{e=
human HepG2 cells MWjGeY5kfGmxbjDhd5NigQ>? MUXBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKZYDHNkBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHPlcIwh\3Kxd4ToJIJ6KE2WVDDhd5NigSxiSVO1NF01OC56NzFOwG0> MWKyOVE3ODh|Nx?=
human ECA109 cells MXnDfZRwfG:6aXRCpIF{e2G7 NHvIcoY4OiCq Mn3RR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gSWNCOTB7IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9OFIvPiEQvF2= NVnNbWlTOjB4N{O3Nlc>
human BCG823 cells M2fXVWN6fG:2b4jpZ:Kh[XO|YYm= NYXQ[Ix4S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSkOJOEKzJINmdGy|IHL5JG1VXCCjc4PhfUwhUUN3ME20OE43KM7:TR?= NWrrU|JSOTh7NUK0NlA>
human KB cells M2D3XGN6fG:2b4jpZ:Kh[XO|YYm= NXrUfm46PzJiaB?= Mnn3R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT2Ih[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD12NT6yJO69VQ>? M{HNU|IxPjd|N{K3
human K562 cells MVrDfZRwfG:6aXRCpIF{e2G7 NYPQPZl2PzJiaB?= NGXDPIdEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBMPTZ{IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9OFYh|ryP NHT0eZAzODZ5M{eyOy=>
rat VSMC MUPGeY5kfGmxbjDhd5NigQ>? NWPMXVRuPSEQvF2= MlPvN|AhdWmwcx?= MkTpSIVkemWjc3WgbY4hcW62cnHj[YxtfWyjcjDSU3MhdGW4ZXygbY4hWESJRj3zeIlufWyjdHXkJJJifCCYU13DJIF1KDVidV2gdJJmfHKnYYTl[EB4cXSqIHPvcZBwfW6mIH\vdkA{OCCvaX7zJI1m[XO3cnXkJIFnfGW{IIXwJJRwKDJ|IHjyd{Bw\iCyb4P0JJN1cW23bHH0bY9vKHW|aX7nJGgzTEOIIHL5JIZtd3diY4n0c41mfHKrYzDhcoFtgXOrcx?= MVSyNVI3PTV3NB?=
rat PC12 cells MmWzSpVv[3Srb36gZZN{[Xl? MnTGNk42KM7:TR?= NV\TfJR4OyCq MXPO[ZVzd3C{b4TlZ5RqfmViYXP0bZZqfHliYXfhbY5{fCCKMl:yMYlv\HWlZXSg[IFu[WenIHnuJJJifCCSQ{GyJINmdGy|IHHzd4V{e2WmIHHzJINmdGxidnnhZoltcXS7IHH0JFIvPSC3TTDwdoVqdmO3YnH0[YQh\m:{IEOgbJJ{KG[xbHzve4VlKGK7IFiyU|Ih[2ijbHzlcodmKG2nYYP1doVlKGGodHXyJFUhcHK|IHL5JG1VXCCjc4PhfS=> MlrSNlQ3QTd|M{W=

... Click to View More Cell Line Experimental Data

In vivo In vivo, Caffeic acid phenethyl ester (10 mg/kg, i.p.) inhibits the growth and angiogenesis of primary tumors in C57BL/6 and BALB/c mice inoculated with Lewis lung carcinoma, colon carcinoma, and melanoma cells. [5] Caffeic acid phenethyl ester (5, 10, 20 mg/kg) also shows immunomodulatory effects in vivo by decreasing thymus weight and/or cellularity of thymus and spleen. [6]

Protocol

Cell Research:

[2]

+ Expand
  • Cell lines: Murine colon 26-L5, murine B16-BL6 melanoma, human HT-1080 fibrosarcoma and human lung A549 adenocarcinoma cell lines
  • Concentrations: ~200 μM
  • Incubation Time: 3 days
  • Method:

    Human HT-1080 fibrosarcoma, human lung A549 adenocarcinoma and murine B16-BL6 melanoma cell lines are maintained in EMEM medium supplemented with 10% FCS, 0.1% sodium bicarbonate and 2 mM glutamine. Murine colon 26-L5 carcinoma cell line, on the other hand, is maintained in RPMI medium containing the same supplements as in EMEM. These are all highly metastatic cell lines except for A-549 carcinoma. Cellular viability is determined using the standard MTT assay. In brief, exponentially growing cells are harvested and 100 μl of cell suspension containing 2000 cells is plated in 96-well microtiter plates. After 24 h of incubation to allow for cell attachment, the cells are treated with varying concentrations of test samples in medium (100 μl) and incubated for 72 h at 37°C under 5% CO2. Three hours after the addition of MTT, the amount of formazan formed is measured spectrophotometrically at 550 nm with a Perkin Elmer HTS-7000 plate reader. The test samples are first dissolved in DMSO and then diluted with medium; the final concentration of DMSO is less than 0.25%. Normal also had the same extent of DMSO. 5-Fluorouracil (5-FU) and doxorubicin HCl are used as positive controls, and EC50 values are calculated from the mean values of data from 4 wells.


    (Only for Reference)
Animal Research:

[5]

+ Expand
  • Animal Models: C57BL/6 and BALB/c mice inoculated with Lewis lung carcinoma, colon carcinoma, and melanoma cells
  • Formulation: PBS
  • Dosages: ~10 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 57 mg/mL (200.48 mM)
Ethanol 57 mg/mL (200.48 mM)
Water <1 mg/mL

* 1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 284.31
Formula

C17H16O4

CAS No. 104594-70-9
Storage powder
in solvent
Synonyms CAPE, Phenylethyl Caffeate

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02393755 Recruiting Colon Adenocarcinoma|Rectal Adenocarcinoma|Recurrent Colon Carcinoma|Recurrent Rectal Carcinoma|Stage IVA Colon Cancer|Stage IVA Rectal Cancer|Stage IVB Colon Cancer|Stage IVB Rectal Cancer Roswell Park Cancer Institute|National Cancer Institute (NCI)|Boehringer Ingelheim|National Comprehensive Cancer Network May 8, 2015 Phase 1|Phase 2
NCT02352831 Recruiting Pancreatic Cancer|Cancer of Pancreas|Cancer of the Pancreas|Pancreas Cancer Washington University School of Medicine August 31, 2015 Phase 1|Phase 2
NCT03050814 Not yet recruiting Colorectal Cancer National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) February 3, 2017 Phase 2
NCT01497392 Completed Adenocarcinoma of the Pancreas|Stage III Pancreatic Cancer|Stage IV Pancreatic Cancer|Unspecified Adult Solid Tumor, Protocol Specific Roswell Park Cancer Institute|National Cancer Institute (NCI)|Novartis March 29, 2012 Phase 1
NCT01134601 Terminated Non-Metastatic Adenocarcinoma of the Rectum National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) May 24, 2010 Phase 1
NCT02954055 Not yet recruiting Breast Cancer International Breast Cancer Study Group May 2017 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

NF-κB Signaling Pathway Map

Related NF-κB Products

Tags: buy Caffeic Acid Phenethyl Ester | Caffeic Acid Phenethyl Ester supplier | purchase Caffeic Acid Phenethyl Ester | Caffeic Acid Phenethyl Ester cost | Caffeic Acid Phenethyl Ester manufacturer | order Caffeic Acid Phenethyl Ester | Caffeic Acid Phenethyl Ester distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID