Caffeic Acid Phenethyl Ester

Catalog No.S7414 Synonyms: CAPE, Phenylethyl Caffeate

Caffeic Acid Phenethyl Ester Chemical Structure

Molecular Weight(MW): 284.31

Caffeic acid phenethyl ester is a potent and specific inhibitor of NF-κB activation, and also displays antioxidant, immunomodulatory and antiinflammatory activities.

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3 Customer Reviews

  • The protein expression alteration of p-NF-κB, NF-κB, PD-L1 in NP-69-LMP1 or NP-69 cell lines treated with 0, 0.5, and 1.0 μM Caffeic Acid Phenethyl Ester (CAPE) for 72 hours. β-actin was used to verify equal loading.

    Oncotarget, 2014, 5(23): 12189-202 . Caffeic Acid Phenethyl Ester purchased from Selleck.

    Representative apoptosis assay of LPS141 cells. Summary of apoptosis assay results. Data represent % apoptotic cells ± standard deviation (SD, error bars).

    Sci Rep, 2015, 5: 12580. Caffeic Acid Phenethyl Ester purchased from Selleck.

  • PC cells were treated with 5 μM CAPE for 24 h, and EMT markers were detected (n=3/group). * P < 0.05, ** P < 0.01, and *** P < 0.001

    Oncotarget, 2016, 7(25):38539-38550. Caffeic Acid Phenethyl Ester purchased from Selleck.

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Biological Activity

Description Caffeic acid phenethyl ester is a potent and specific inhibitor of NF-κB activation, and also displays antioxidant, immunomodulatory and antiinflammatory activities.
Targets
NF-κB [1]
In vitro

Caffeic acid phenethyl ester blocks NF-κB activation induced by phorbol ester, ceramide, okadaic acid, and hydrogen peroxide by preventing the translocation of the p65 subunit of NF-κB to the nucleus. [1] In a series of tumor cell lines, Caffeic acid phenethyl ester shows promising antiproliferative activity with EC50 of 1.76, 3.16, 13.7, and 44.0 μM against murine colon 26-L5, murine B16-BL6 melanoma, human HT-1080 fibrosarcoma and human lung A549 adenocarcinoma cell lines, respectively. [2] Caffeic acid phenethyl ester, as a potent antioxidant, exerts its anti-apoptotic effect in cerebellar granule cells by blocking ROS formation and inhibiting caspase activity. [3] Moreover, Caffeic acid phenethyl ester attenuates the pro-inflammatory phenotype of LPS-stimulated HSCs, and LPS-induced sensitization of HSCs to fibrogenic cytokines by inhibiting NF-κB signaling. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse RAW264.7 cells MYjGeY5kfGmxbjDhd5NigQ>? NEi1dHAzPCCq M1W0bWlvcGmkaYTpc44hd2ZiTGDTMYlv\HWlZXSgcol1emmlIH;4bYRmKHC{b3T1Z5Rqd25iaX6gcY92e2ViUlHXNlY1NjdiY3XscJMh[XO|ZYPz[YQh[XNibnn0dol1\SCjY3P1cZVt[XSrb36gZYRucW6rc4TldoVlKDFiaIKgZoVnd3KnIFzQV{BkcGGubHXu[4Uh[W6mIH3lZZN2emWmIHHmeIVzKDJ2IHjyd{BjgSCJcnnld5MhemWjZ3XueEBie3OjeTygSWM2OD1yLkG5N{DPxE1? NFHZOWEyQDZ4N{OyNC=>
mouse 26-L5 cells M3;tSnBzd2yrZnXyZZRqd25iYYPzZZk> MkHuO|IhcA>? NG\qSI9CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JI1wfXOnIEK2MWw2KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGVEPTB;MD6zJO69VQ>? MlHZNVIxOjd5M{m=
human HeLa cells MV;Qdo9tcW[ncnH0bY9vKGG|c3H5 MXO3NkBp MnvPRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKZVzhJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFXDOVA:Oi5|NjFOwG0> NIfuZ5QyOjB{N{ezPS=>
human Bewo cells MW\GeY5kfGmxbjDhd5NigQ>? M3XWO2FvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFLle48h[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDj[YxtKGe{b4f0bEBjgSCPVGSgZZN{[XluIFnDOVA:Oi57NjFOwG0> MV[yOVE3ODh|Nx?=
mouse J774.1 cells MVfGeY5kfGmxbjDhd5NigQ>? MknONlQhcA>? M2GzT2lvcGmkaYTpc44hd2ZiTGDTMYlv\HWlZXSgUm8heHKxZIXjeIlwdiCrbjDtc5V{\SCMN{e0MlEh[2WubIOgZYZ1\XJiMkSgbJJ{KGK7IFfybYV{eyC{ZXHn[Y51KGG|c3H5MEBKSzVyPUSuPEDPxE1? MmHjNVU6PzR4MEi=
human Bel7402 cells M1jsT2N6fG:2b4jpZ:Kh[XO|YYm= NWSzVpc1S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSmWuN{SwNkBk\WyuczDifUBOXFRiYYPzZZktKEmFNUC9OU42KM7:TR?= MnrQNVg6PTJ2MkC=
human LNCAP cells NIDW[JJHfW6ldHnvckBie3OjeR?= M37LZ|I1KGh? NFjQT5dCdnSrYX7kdo9o\W6rYzDhZ5Rqfmm2eTDpckBpfW2jbjDMUmNCWCClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gSGhVNXO2aX31cIF1\WRiUGPBJJJmdGWjc3WgZYZ1\XJiMkSgbJJ{KGK7IFXMTXNCNCCLQ{WwQVYvOiCwTR?= MXiyOFA5ODFyNR?=
mouse BV2 cells NXfiU217TnWwY4Tpc44h[XO|YYm= MljDNlQhcA>? MYXJcohq[mm2aX;uJI9nKG6rdILpZ{BwgGmmZTDwdo9lfWO2aX;uJIlvKEySUz3zeIlufWyjdHXkJI1wfXOnIFLWNkBk\WyuczD0doVifGWmIEOgbJJ{KGKnZn;y[UBNWFNiYXTkbZRqd25ibXXhd5Vz\WRiYX\0[ZIhOjRiaILzJIJ6KEe{aXXzd{Bie3OjeTygTWM2OD14LkSg{txO M1rwNlI{QDdyN{Cw
mouse B16-BL6 cells Mmq5VJJwdGmoZYLheIlwdiCjc4PhfS=> NUnjfmVlPzJiaB?= NETaPGlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JI1wfXOnIFKxOk1DVDZiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhTUN3ME22Mlc6KM7:TR?= MUixNlAzPzd|OR?=
HUVEC cells NHrDRWdEgXSxdH;4bYPDqGG|c3H5 NIfTcmoyQCCq NUHlUI9RS3m2b4Dyc5Rm[3SrdnWgZYN1cX[rdImgZYdicW6|dDDINm8zNWmwZIXj[YQhd3irZHH0bZZmKHO2cnXzd{BqdiCKVW\FR{Bk\WyuczDh[pRmeiBzODDodpMh[nliQ3XscE1VcXSncjDCcJVmKGG|c3H5 NE\DcnIzODV7OEi5OC=>
human HT1080 cells NV\HNGNGWHKxbHnm[ZJifGmxbjDhd5NigQ>? MWq3NkBp MkfvRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKVEGwPFAh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygSWM2OD17LkWg{txO NY\DTmwxOTJyMke3N|k>
human HL60 cells NVOxNlJ3S3m2b4TvfIlkyqCjc4PhfS=> NVTkOJhIPzJiaB?= NIDwfm1EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJVDZyIHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9PU45KM7:TR?= NHPabokzODZ5M{eyOy=>
human LS 174T cells NIjmWY9EgXSxdH;4bYPDqGG|c3H5 NH;yOos4OiCq MkPqR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUHMhOTd2VDDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{[wQVkvQSEQvF2= MlnzNlA3PzN5Mke=
human SGC7901 cells MofkSpVv[3Srb36gZZN{[Xl? M4iwZ2FvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGPHR|c6ODFiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBk\WyuIHfyc5d1cCCkeTDNWHQh[XO|YYmsJGlEPTB;MUSuNlYh|ryP Mm\UNlUyPjB6M{e=
human Bel7404 cells Mni4R5l1d3SxeHnjxsBie3OjeR?= NWX4V21DPzJiaB?= NGPOeYdEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBD\Wx5NEC0JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OTRwNTFOwG0> M2X5ZlIxPjd|N{K3
human BGC823 cells M2m5dmN6fG:2b4jpZ:Kh[XO|YYm= MUW3NkBp MmO3R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRmdEQDJ|IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9NVkvOyEQvF2= NH21NoMzODZ5M{eyOy=>
human HO8910 cells MmTBR5l1d3SxeHnjxsBie3OjeR?= NXWwUoNSPzJiaB?= NV;FRY5PS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUE96OUGwJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OjVwNTFOwG0> NH7YRogzODZ5M{eyOy=>
human MCF7 cells MXHDfZRwfG:6aXRCpIF{e2G7 MlrER5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{BjgSCPVGSgZZN{[XluIFnDOVA:OjZwNzFOwG0> NIfScI4yQDl3MkSyNC=>
human A549 cells NVvqOlF7S3m2b4TvfIlkyqCjc4PhfS=> NVrKWmFSPzJiaB?= NVy4TmRpS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVI3NjhizszN NHriUYMzODZ5M{eyOy=>
human LNCAP cells MWDDfZRwfG:6aXRCpIF{e2G7 Mny2NlQhcA>? NEfGeItEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBNVkODUDDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4h[2WubDD2bYFjcWyrdImgZYZ1\XJiMkSgbJJ{KGK7IGfTWE0yKGG|c3H5MEBKSzVyPUOzMlch|ryP MWGyOFA5ODFyNR?=
human HeLa cells M1\TfmZ2dmO2aX;uJIF{e2G7 MnzXRY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSHXMZUBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHPlcIwh\3Kxd4ToJIJ6KE2WVDDhd5NigSxiSVO1NF0{Py57ODFOwG0> NEey[GYzPTF4MEizOy=>
human HepG2 cells NXnMcZBFTnWwY4Tpc44h[XO|YYm= NXewZldESW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gTIVxTzJiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBk\WyuIHfyc5d1cCCkeTDNWHQh[XO|YYmsJGlEPTB;NECuPFch|ryP MWCyOVE3ODh|Nx?=
human ECA109 cells NVLSWY5WS3m2b4TvfIlkyqCjc4PhfS=> MUm3NkBp NF;jSY1EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBGS0FzMEmgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF01Oi54IN88US=> M3[yV|IxPjd|N{K3
human BCG823 cells MX;DfZRwfG:6aXRCpIF{e2G7 M1XuNmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGJETzh{MzDj[YxteyCkeTDNWHQh[XO|YYmsJGlEPTB;NESuOkDPxE1? NGDvdXcyQDl3MkSyNC=>
human KB cells NEXyeoZEgXSxdH;4bYPDqGG|c3H5 NVzSbVFVPzJiaB?= NES2OXlEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBMSiClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUS1MlIh|ryP MnvuNlA3PzN5Mke=
human K562 cells NVH6bYtNS3m2b4TvfIlkyqCjc4PhfS=> NUTJXIdoPzJiaB?= NU\vOHdzS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUzV4MjDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVQ3KM7:TR?= NGnYV4kzODZ5M{eyOy=>
rat VSMC M4m1SGZ2dmO2aX;uJIF{e2G7 Ml:3OUDPxE1? M{DhUlMxKG2rboO= M4KwZmRm[3KnYYPlJIlvKGmwdILhZ4VtdHWuYYKgVm9UKGyndnXsJIlvKFCGR1[td5RqdXWuYYTl[EBz[XRiVmPNR{BifCB3IIXNJJBz\XS{ZXH0[YQhf2m2aDDjc41xd3WwZDDmc5IhOzBibXnud{Bu\WG|dYLl[EBi\nSncjD1dEB1dyB{MzDodpMhd2ZicH;zeEB{fGmvdXzheIlwdiC3c3nu[{BJOkSFRjDifUBndG:5IHP5eI9u\XS{aXOgZY5idHm|aYO= NXnvR|BJOjF{NkW1OVQ>
rat PC12 cells NV7CSIVWTnWwY4Tpc44h[XO|YYm= MnfRNk42KM7:TR?= NWDKcWhCOyCq MVfO[ZVzd3C{b4TlZ5RqfmViYXP0bZZqfHliYXfhbY5{fCCKMl:yMYlv\HWlZXSg[IFu[WenIHnuJJJifCCSQ{GyJINmdGy|IHHzd4V{e2WmIHHzJINmdGxidnnhZoltcXS7IHH0JFIvPSC3TTDwdoVqdmO3YnH0[YQh\m:{IEOgbJJ{KG[xbHzve4VlKGK7IFiyU|Ih[2ijbHzlcodmKG2nYYP1doVlKGGodHXyJFUhcHK|IHL5JG1VXCCjc4PhfS=> NUHycXdrOjR4OUezN|U>

... Click to View More Cell Line Experimental Data

In vivo In vivo, Caffeic acid phenethyl ester (10 mg/kg, i.p.) inhibits the growth and angiogenesis of primary tumors in C57BL/6 and BALB/c mice inoculated with Lewis lung carcinoma, colon carcinoma, and melanoma cells. [5] Caffeic acid phenethyl ester (5, 10, 20 mg/kg) also shows immunomodulatory effects in vivo by decreasing thymus weight and/or cellularity of thymus and spleen. [6]

Protocol

Cell Research:

[2]

+ Expand
  • Cell lines: Murine colon 26-L5, murine B16-BL6 melanoma, human HT-1080 fibrosarcoma and human lung A549 adenocarcinoma cell lines
  • Concentrations: ~200 μM
  • Incubation Time: 3 days
  • Method:

    Human HT-1080 fibrosarcoma, human lung A549 adenocarcinoma and murine B16-BL6 melanoma cell lines are maintained in EMEM medium supplemented with 10% FCS, 0.1% sodium bicarbonate and 2 mM glutamine. Murine colon 26-L5 carcinoma cell line, on the other hand, is maintained in RPMI medium containing the same supplements as in EMEM. These are all highly metastatic cell lines except for A-549 carcinoma. Cellular viability is determined using the standard MTT assay. In brief, exponentially growing cells are harvested and 100 μl of cell suspension containing 2000 cells is plated in 96-well microtiter plates. After 24 h of incubation to allow for cell attachment, the cells are treated with varying concentrations of test samples in medium (100 μl) and incubated for 72 h at 37°C under 5% CO2. Three hours after the addition of MTT, the amount of formazan formed is measured spectrophotometrically at 550 nm with a Perkin Elmer HTS-7000 plate reader. The test samples are first dissolved in DMSO and then diluted with medium; the final concentration of DMSO is less than 0.25%. Normal also had the same extent of DMSO. 5-Fluorouracil (5-FU) and doxorubicin HCl are used as positive controls, and EC50 values are calculated from the mean values of data from 4 wells.


    (Only for Reference)
Animal Research:

[5]

+ Expand
  • Animal Models: C57BL/6 and BALB/c mice inoculated with Lewis lung carcinoma, colon carcinoma, and melanoma cells
  • Formulation: PBS
  • Dosages: ~10 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 57 mg/mL (200.48 mM)
Ethanol 57 mg/mL (200.48 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 284.31
Formula

C17H16O4

CAS No. 104594-70-9
Storage powder
in solvent
Synonyms CAPE, Phenylethyl Caffeate

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02393755 Recruiting Colon Adenocarcinoma|Rectal Adenocarcinoma|Recurrent Colon Carcinoma|Recurrent Rectal Carcinoma|Stage IVA Colon Cancer|Stage IVA Rectal Cancer|Stage IVB Colon Cancer|Stage IVB Rectal Cancer Roswell Park Cancer Institute|National Cancer Institute (NCI)|Boehringer Ingelheim|National Comprehensive Cancer Network May 8, 2015 Phase 1|Phase 2
NCT02352831 Recruiting Pancreatic Cancer|Cancer of Pancreas|Cancer of the Pancreas|Pancreas Cancer Washington University School of Medicine August 31, 2015 Phase 1|Phase 2
NCT03050814 Not yet recruiting Colorectal Cancer National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) February 3, 2017 Phase 2
NCT01497392 Completed Adenocarcinoma of the Pancreas|Stage III Pancreatic Cancer|Stage IV Pancreatic Cancer|Unspecified Adult Solid Tumor, Protocol Specific Roswell Park Cancer Institute|National Cancer Institute (NCI)|Novartis March 29, 2012 Phase 1
NCT01134601 Terminated Non-Metastatic Adenocarcinoma of the Rectum National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) May 24, 2010 Phase 1
NCT02954055 Not yet recruiting Breast Cancer International Breast Cancer Study Group May 2017 Phase 2

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID