Caffeic Acid Phenethyl Ester

Catalog No.S7414 Synonyms: CAPE, Phenylethyl Caffeate

Caffeic Acid Phenethyl Ester Chemical Structure

Molecular Weight(MW): 284.31

Caffeic acid phenethyl ester is a potent and specific inhibitor of NF-κB activation, and also displays antioxidant, immunomodulatory and antiinflammatory activities.

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3 Customer Reviews

  • The protein expression alteration of p-NF-κB, NF-κB, PD-L1 in NP-69-LMP1 or NP-69 cell lines treated with 0, 0.5, and 1.0 μM Caffeic Acid Phenethyl Ester (CAPE) for 72 hours. β-actin was used to verify equal loading.

    Oncotarget, 2014, 5(23): 12189-202 . Caffeic Acid Phenethyl Ester purchased from Selleck.

    Representative apoptosis assay of LPS141 cells. Summary of apoptosis assay results. Data represent % apoptotic cells ± standard deviation (SD, error bars).

    Sci Rep, 2015, 5: 12580. Caffeic Acid Phenethyl Ester purchased from Selleck.

  • PC cells were treated with 5 μM CAPE for 24 h, and EMT markers were detected (n=3/group). * P < 0.05, ** P < 0.01, and *** P < 0.001

    Oncotarget, 2016, 7(25):38539-38550. Caffeic Acid Phenethyl Ester purchased from Selleck.

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Biological Activity

Description Caffeic acid phenethyl ester is a potent and specific inhibitor of NF-κB activation, and also displays antioxidant, immunomodulatory and antiinflammatory activities.
Targets
NF-κB [1]
In vitro

Caffeic acid phenethyl ester blocks NF-κB activation induced by phorbol ester, ceramide, okadaic acid, and hydrogen peroxide by preventing the translocation of the p65 subunit of NF-κB to the nucleus. [1] In a series of tumor cell lines, Caffeic acid phenethyl ester shows promising antiproliferative activity with EC50 of 1.76, 3.16, 13.7, and 44.0 μM against murine colon 26-L5, murine B16-BL6 melanoma, human HT-1080 fibrosarcoma and human lung A549 adenocarcinoma cell lines, respectively. [2] Caffeic acid phenethyl ester, as a potent antioxidant, exerts its anti-apoptotic effect in cerebellar granule cells by blocking ROS formation and inhibiting caspase activity. [3] Moreover, Caffeic acid phenethyl ester attenuates the pro-inflammatory phenotype of LPS-stimulated HSCs, and LPS-induced sensitization of HSCs to fibrogenic cytokines by inhibiting NF-κB signaling. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse RAW264.7 cells MlTYSpVv[3Srb36gZZN{[Xl? NGTsfYkzPCCq NYnXdVFpUW6qaXLpeIlwdiCxZjDMVHMucW6mdXPl[EBvcXS{aXOgc5hq\GVicILv[JVkfGmxbjDpckBud3W|ZTDSRXczPjRwNzDj[YxteyCjc4Pld5Nm\CCjczDubZRzcXSnIHHjZ5VufWyjdHnvckBi\G2rbnnzeIVz\WRiMTDodkBj\W[xcnWgUHBUKGOqYXzs[Y5o\SCjbnSgcYVie3W{ZXSgZYZ1\XJiMkSgbJJ{KGK7IFfybYV{eyC{ZXHn[Y51KGG|c3H5MEBGSzVyPUCuNVk{KM7:TR?= M4DvPFE5PjZ5M{Kw
mouse 26-L5 cells NHLSVFdRem:uaX\ldoF1cW:wIHHzd4F6 NVmwdFdVPzJiaB?= NGnFcGtCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JI1wfXOnIEK2MWw2KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGVEPTB;MD6zJO69VQ>? MmPONVIxOjd5M{m=
human HeLa cells M{PCVHBzd2yrZnXyZZRqd25iYYPzZZk> MWW3NkBp NI[wcZhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjlUIEh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygSWM2OD1{LkO2JO69VQ>? NFzvepUyOjB{N{ezPS=>
human Bewo cells MUfGeY5kfGmxbjDhd5NigQ>? MV;BcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDoeY1idiCEZYfvJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiY3XscEBoem:5dHigZpkhVVSWIHHzd4F6NCCLQ{WwQVIvQTZizszN MmPTNlUyPjB6M{e=
mouse J774.1 cells NETadWlHfW6ldHnvckBie3OjeR?= M4fPe|I1KGh? NGDSdpFKdmirYnn0bY9vKG:oIFzQV{1qdmS3Y3XkJG5QKHC{b3T1Z5Rqd25iaX6gcY92e2ViSke3OE4yKGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDHdolme3NicnXh[4VvfCCjc4PhfUwhUUN3ME20Mlgh|ryP MmXuNVU6PzR4MEi=
human Bel7402 cells MV;DfZRwfG:6aXRCpIF{e2G7 M{HLWWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGJmdDd2MEKgZ4VtdHNiYomgUXRVKGG|c3H5MEBKSzVyPUWuOUDPxE1? MVWxPFk2OjR{MB?=
human LNCAP cells M4\lU2Z2dmO2aX;uJIF{e2G7 MUmyOEBp NUTDcHpOSW62aXHu[JJw\2WwaXOgZYN1cX[rdImgbY4hcHWvYX6gUG5ESVBiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJGRJXC2|dHnteYxifGWmIGDTRUBz\WynYYPlJIFnfGW{IEK0JIhzeyCkeTDFUGlUSSxiSVO1NF03NjJibl2= NFnIN24zPDB6MEGwOS=>
mouse BV2 cells M3n4XGZ2dmO2aX;uJIF{e2G7 M2\HSVI1KGh? M{HGVGlvcGmkaYTpc44hd2Zibnn0dolkKG:6aXTlJJBzd2S3Y4Tpc44hcW5iTGDTMZN1cW23bHH0[YQhdW:3c3WgRnYzKGOnbHzzJJRz\WG2ZXSgN{BpenNiYnXmc5JmKEySUzDh[IRqfGmxbjDt[YF{fXKnZDDh[pRmeiB{NDDodpMh[nliR4Lp[ZN{KGG|c3H5MEBKSzVyPU[uOEDPxE1? NGe2UmIzOzh5MEewNC=>
mouse B16-BL6 cells NWTlV3c6WHKxbHnm[ZJifGmxbjDhd5NigQ>? NF;Db4w4OiCq MmLuRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDtc5V{\SCEMU[tRmw3KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGVEPTB;Nj63PUDPxE1? MYOxNlAzPzd|OR?=
HUVEC cells NU\BO4NES3m2b4TvfIlkyqCjc4PhfS=> MlfTNVghcA>? M4HmU2N6fG:ycn;0[YN1cX[nIHHjeIl3cXS7IHHnZYlve3RiSELPNk1qdmS3Y3XkJI95cWSjdHn2[UB{fHKnc4OgbY4hUFWYRVOgZ4VtdHNiYX\0[ZIhOThiaILzJIJ6KEOnbHytWIl1\XJiQnz1[UBie3OjeR?= MlPmNlA2QTh6OUS=
human HT1080 cells Mnz5VJJwdGmoZYLheIlwdiCjc4PhfS=> NGq4XVk4OiCq MVnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiWMUC4NEBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDFR|UxRTlwNTFOwG0> NYrNd3MzOTJyMke3N|k>
human HL60 cells Mn7hR5l1d3SxeHnjxsBie3OjeR?= MnrqO|IhcA>? NV70WHlGS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEx4MDDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVkvQCEQvF2= NUDNfWhnOjB4N{O3Nlc>
human LS 174T cells MnG1R5l1d3SxeHnjxsBie3OjeR?= M3\pNFczKGh? MnvBR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUHMhOTd2VDDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{[wQVkvQSEQvF2= MYKyNFY4Ozd{Nx?=
human SGC7901 cells MVHGeY5kfGmxbjDhd5NigQ>? NWC2N|JoSW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gV2dEPzlyMTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKGOnbHyg[5Jwf3SqIHL5JG1VXCCjc4PhfUwhUUN3ME2xOE4zPiEQvF2= M3riNVI2OTZyOEO3
human Bel7404 cells MmHLR5l1d3SxeHnjxsBie3OjeR?= NFjoPIY4OiCq MXrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDC[Yw4PDB2IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9NVQvPSEQvF2= NYHqcI82OjB4N{O3Nlc>
human BGC823 cells NVyy[ZhvS3m2b4TvfIlkyqCjc4PhfS=> M4G5VVczKGh? M2eybmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGJISzh{MzDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVE6NjNizszN NUO3RWVMOjB4N{O3Nlc>
human HO8910 cells NELwW2lEgXSxdH;4bYPDqGG|c3H5 NF3nPHY4OiCq MmraR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTG85QTFyIHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9NlUvPSEQvF2= NHTnO2czODZ5M{eyOy=>
human MCF7 cells MVvDfZRwfG:6aXRCpIF{e2G7 MWHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIJ6KE2WVDDhd5NigSxiSVO1NF0zPi55IN88US=> NGHuWYEyQDl3MkSyNC=>
human A549 cells MWPDfZRwfG:6aXRCpIF{e2G7 NUG2VJJ{PzJiaB?= MkXhR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUK2Mlgh|ryP MUeyNFY4Ozd{Nx?=
human LNCAP cells M3raO2N6fG:2b4jpZ:Kh[XO|YYm= NHPyWGIzPCCq M4HuTGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGxPS0GSIHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDh[pRmeiB{NDDodpMh[nliV2PUMVEh[XO|YYmsJGlEPTB;M{OuO{DPxE1? M1XhPVI1ODhyMUC1
human HeLa cells M{Hzb2Z2dmO2aX;uJIF{e2G7 NVfHRoZTSW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gTIVN[SClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJINmdGxiZ4Lve5RpKGK7IF3UWEBie3OjeTygTWM2OD1|Nz65PEDPxE1? NYTlclNDOjVzNkC4N|c>
human HepG2 cells MVPGeY5kfGmxbjDhd5NigQ>? MWnBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKZYDHNkBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHPlcIwh\3Kxd4ToJIJ6KE2WVDDhd5NigSxiSVO1NF01OC56NzFOwG0> NWLGWWg3OjVzNkC4N|c>
human ECA109 cells MWDDfZRwfG:6aXRCpIF{e2G7 MmjiO|IhcA>? MX7DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDFR2EyODliY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME20Nk43KM7:TR?= MXGyNFY4Ozd{Nx?=
human BCG823 cells MlvvR5l1d3SxeHnjxsBie3OjeR?= MVfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDCR2c5OjNiY3XscJMh[nliTWTUJIF{e2G7LDDJR|UxRTR2Lk[g{txO M2\WeFE5QTV{NEKw
human KB cells NXS2SJBzS3m2b4TvfIlkyqCjc4PhfS=> NHntNWk4OiCq M3\VWmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGtDKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;NEWuNkDPxE1? M4DZUlIxPjd|N{K3
human K562 cells NHrnSJdEgXSxdH;4bYPDqGG|c3H5 Ml;yO|IhcA>? Mk\0R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT|U3OiClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUS2JO69VQ>? NGTM[JkzODZ5M{eyOy=>
rat VSMC M4nJOWZ2dmO2aX;uJIF{e2G7 MkG1OUDPxE1? M3LqdFMxKG2rboO= MofzSIVkemWjc3WgbY4hcW62cnHj[YxtfWyjcjDSU3MhdGW4ZXygbY4hWESJRj3zeIlufWyjdHXkJJJifCCYU13DJIF1KDVidV2gdJJmfHKnYYTl[EB4cXSqIHPvcZBwfW6mIH\vdkA{OCCvaX7zJI1m[XO3cnXkJIFnfGW{IIXwJJRwKDJ|IHjyd{Bw\iCyb4P0JJN1cW23bHH0bY9vKHW|aX7nJGgzTEOIIHL5JIZtd3diY4n0c41mfHKrYzDhcoFtgXOrcx?= MY[yNVI3PTV3NB?=
rat PC12 cells MVnGeY5kfGmxbjDhd5NigQ>? NIDFPVQzNjVizszN M4Lxe|MhcA>? NWD6box2VmW3cn;wdo91\WO2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgTFJQOi2rbnT1Z4VlKGSjbXHn[UBqdiC{YYSgVGMyOiClZXzsd{Bie3Onc4Pl[EBieyClZXzsJJZq[WKrbHn0fUBifCB{LkWgeW0heHKnaX7jeYJifGWmIH\vdkA{KGi{czDmc4xtd3enZDDifUBJOk9{IHPoZYxt\W6pZTDt[YF{fXKnZDDh[pRmeiB3IHjyd{BjgSCPVGSgZZN{[Xl? MnPFNlQ3QTd|M{W=

... Click to View More Cell Line Experimental Data

In vivo In vivo, Caffeic acid phenethyl ester (10 mg/kg, i.p.) inhibits the growth and angiogenesis of primary tumors in C57BL/6 and BALB/c mice inoculated with Lewis lung carcinoma, colon carcinoma, and melanoma cells. [5] Caffeic acid phenethyl ester (5, 10, 20 mg/kg) also shows immunomodulatory effects in vivo by decreasing thymus weight and/or cellularity of thymus and spleen. [6]

Protocol

Cell Research:

[2]

+ Expand
  • Cell lines: Murine colon 26-L5, murine B16-BL6 melanoma, human HT-1080 fibrosarcoma and human lung A549 adenocarcinoma cell lines
  • Concentrations: ~200 μM
  • Incubation Time: 3 days
  • Method:

    Human HT-1080 fibrosarcoma, human lung A549 adenocarcinoma and murine B16-BL6 melanoma cell lines are maintained in EMEM medium supplemented with 10% FCS, 0.1% sodium bicarbonate and 2 mM glutamine. Murine colon 26-L5 carcinoma cell line, on the other hand, is maintained in RPMI medium containing the same supplements as in EMEM. These are all highly metastatic cell lines except for A-549 carcinoma. Cellular viability is determined using the standard MTT assay. In brief, exponentially growing cells are harvested and 100 μl of cell suspension containing 2000 cells is plated in 96-well microtiter plates. After 24 h of incubation to allow for cell attachment, the cells are treated with varying concentrations of test samples in medium (100 μl) and incubated for 72 h at 37°C under 5% CO2. Three hours after the addition of MTT, the amount of formazan formed is measured spectrophotometrically at 550 nm with a Perkin Elmer HTS-7000 plate reader. The test samples are first dissolved in DMSO and then diluted with medium; the final concentration of DMSO is less than 0.25%. Normal also had the same extent of DMSO. 5-Fluorouracil (5-FU) and doxorubicin HCl are used as positive controls, and EC50 values are calculated from the mean values of data from 4 wells.


    (Only for Reference)
Animal Research:

[5]

+ Expand
  • Animal Models: C57BL/6 and BALB/c mice inoculated with Lewis lung carcinoma, colon carcinoma, and melanoma cells
  • Formulation: PBS
  • Dosages: ~10 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 57 mg/mL (200.48 mM)
Ethanol 57 mg/mL (200.48 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 284.31
Formula

C17H16O4

CAS No. 104594-70-9
Storage powder
Synonyms CAPE, Phenylethyl Caffeate

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02393755 Recruiting Colon Adenocarcinoma|Rectal Adenocarcinoma|Recurrent Colon Carcinoma|Recurrent Rectal Carcinoma|Stage IVA Colon Cancer|Stage IVA Rectal Cancer|Stage IVB Colon Cancer|Stage IVB Rectal Cancer Roswell Park Cancer Institute|National Cancer Institute (NCI)|Boehringer Ingelheim|National Comprehensive Cancer Network May 8, 2015 Phase 1|Phase 2
NCT02352831 Recruiting Pancreatic Cancer|Cancer of Pancreas|Cancer of the Pancreas|Pancreas Cancer Washington University School of Medicine August 31, 2015 Phase 1|Phase 2
NCT03050814 Not yet recruiting Colorectal Cancer National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) February 3, 2017 Phase 2
NCT01497392 Completed Adenocarcinoma of the Pancreas|Stage III Pancreatic Cancer|Stage IV Pancreatic Cancer|Unspecified Adult Solid Tumor, Protocol Specific Roswell Park Cancer Institute|National Cancer Institute (NCI)|Novartis March 29, 2012 Phase 1
NCT01134601 Terminated Non-Metastatic Adenocarcinoma of the Rectum National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) May 24, 2010 Phase 1
NCT02954055 Not yet recruiting Breast Cancer International Breast Cancer Study Group May 2017 Phase 2

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID