Catalog No.S7566

IM-12 Chemical Structure

Molecular Weight(MW): 377.41

IM-12 is a selective GSK-3β inhibitor with IC50 of 53 nM, and also enhances canonical Wnt signalling.

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1 Customer Review

  • Inhibition of GSK-3β promotes Runx2 induction. Vascularsmoothmusclecells(VSMCs) were treated with or without IM-12(3 μM) for 24h, and cells treated with β-GP (10 mM) were as a positive control. The cell lysates were immunoblotted with antibodies against Runx2 and β-actin, respectively.

    Exp Cell Res, 2016, 345(2):206-17.. IM-12 purchased from Selleck.

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Biological Activity

Description IM-12 is a selective GSK-3β inhibitor with IC50 of 53 nM, and also enhances canonical Wnt signalling.
GSK-3β [1]
(Cell-free assay)
53 nM
In vitro

In hNPCs, IM-12 inhibits GSK-3β activity with IC50 of 3.8 μM and subsequently increases β-catenin concentration significantly. Through the activation of canonical Wnt signalling pathway, IM-12 promotes the neuronal differentiation of human neural progenitor cells. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
ReNcell VM cells  NVfmOIU2WHKxbHnm[ZJifGmxbjDhd5NigQ>? NF\UVXc{KHWP NF[0S|A4OiCq MonxRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCUZV7j[YxtKF[PIHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDv[kBk\WyuIIDyc4xq\mW{YYTpc44h[XRiMzD1UUBi\nSncjC3NkBpenN? MXGyNFcxQDl|Nx?=
ST14A cells MVnGeY5kfGmxbjDhd5NigQ>? MV[2JIg> M4TZSGlvcGmkaYTpc44hd2ZiR2PLMVMu[mW2YT3t[YRq[XSnZDDXcpQhe2mpbnHsbY5oKGmwIHj1cYFvKFOWMUTBJINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKGGlY4XteYxifGmxbjDv[kBj\XSjLXPhd4VqdiCjcn;1coQhdnWlbHX1d{Bi\nSncjC2JIhzeyCkeTDtbYNzd3Olb4DpZ{BidmGueYPpdy=> NV\pUnlDOjB5MEi5N|c>

... Click to View More Cell Line Experimental Data


Kinase Assay:[1]
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GSK-3β activity assay:

IC50 of new synthesised compound IM-12 to GSK-3β is determined by a luminometric GSK-3β activity assay. Briefly, compounds are tested in different concentrations diluted in assay buffer containing final concentrations of: 4 mM MOPS pH 7.2; 0.4 mM EDTA; 1 mM EGTA; 2.5 mM β-glycerophosphate; 4 mM MgCl2; 40 μM BSA; 0.05 mM DTT. Four microlitres of diluted compounds are added to 25 μM pGS-2 peptide substrate, 20 ng recombinant GSK-3β and 1 μM ATP to a total assay volume of 40 μl. The enzymatic reaction is stopped after 30 min of incubation at 30 °C by adding 40 μl KinaseGlo. The luminometric signal is allowed to stabilise for 10 min and the measured with a Glomax® 96 Microplate Luminometer.
Cell Research:[1]
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  • Cell lines: ReNcell VM cells
  • Concentrations: 3 μM
  • Incubation Time: 72 hours
  • Method: To measure viable cells, 50-100 μL of cell suspension is analyzed using CASY technology with the appropriate program. ReNcell VM cells are seeded at a defined cell number and proliferated for 24 h. Then the medium is changed to proliferation medium with added substances at indicated concentrations. The cell number was determined every 24 h. Cells were exposed to the added drugs during the whole experiment, whereas the media is changed every 24 h.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 75 mg/mL (198.72 mM)
Ethanol 10 mg/mL warmed (26.49 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 377.41


CAS No. 1129669-05-1
Storage powder
Synonyms N/A

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GSK-3 Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID