SB216763

Catalog No.S1075

SB216763 Chemical Structure

Molecular Weight(MW): 371.22

SB216763 is a potent and selective GSK-3 inhibitor with IC50 of 34.3 nM for GSK-3α and equally effective at inhibiting human GSK-3β.

Size Price Stock Quantity  
In DMSO USD 165 In stock
USD 147 In stock
USD 270 In stock
USD 870 In stock
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3 Customer Reviews

  • Mol Cancer Ther 2014 13(1), 37-48. SB216763 purchased from Selleck.

    Viability assays for five additional GSK3 inhibitors in SY5Y cells.

    Mol Cancer Ther, 2013, 13(2): 454-67 . SB216763 purchased from Selleck.

  • In the presence of SB216763 (2 nM), transwell migration and invasion assay was conducted to evaluate the cell metastasis after Serpine2 transfection. Data were from three independent experiments and were average ± S.E. values. **P < 0.01, compared to transfected vector cells; ##P < 0.01, compared to transfected Serpine2 cells.

    Am J Transl Res, 2016, 8(5):1985-97.. SB216763 purchased from Selleck.

Purity & Quality Control

Choose Selective GSK-3 Inhibitors

Biological Activity

Description SB216763 is a potent and selective GSK-3 inhibitor with IC50 of 34.3 nM for GSK-3α and equally effective at inhibiting human GSK-3β.
Targets
GSK-3α [1]
(Cell-free assay)
GSK-3β [1]
(Cell-free assay)
34.3 nM ~34.3 nM
In vitro

SB 216763 displays similar potency for GSK-3β with 96% inhibition at 10 μM while exhibiting minimal activity against 24 other protein kinases including PKBα and PDK1 with IC50 of >10 μM. SB 216763 stimulates glycogen synthesis in human liver cells with EC50 of 3.6 μM, and induces dose-dependent transcription of a β-catenin-LEF/TCF regulated reporter gene in HEK293 cells with a maximum 2.5-fold induction at 5 μM. [1] SB 216763 protects the cerebellar granule neurones from apoptotic cell death induced by LY-294002 or potassium-deprivation in a concentration-dependent manner, with a maximal neuroprotection at 3 μM in contrast with the effect of lithium chloride at which 10 mM is required. SB 216763 at 3 μM also completely prevents death of chicken dorsal root ganglion sensory neurones induced by LY-294002 regardless of NGF. SB 216763 treatment at 5 μM markedly inhibits the GSK-3-dependent phosphorylation of neuronal-specific microtubule-associated protein tau in cerebellar granule neurones or recombinant tau in HEK293 cells, and induces increased levels of cytoplasmic β-catenin in both cells mimicking the effect of Wnt-mediated inhibition of GSK-3. [2] In pancreatic cancer cell lines including BXPC-3, MIA-PaCa2, PANC1, ASPC1, and CFPAC, SB 216763 treatment at 25-50 μM reduces cell viability in a dose-dependent manner, and leads to significant increase in apoptosis by 50% at 72 hours due to the specific down regulation of GSK-3β, while has no effect in HMEC or WI38 cell lines. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEK293 cells M3HsfWZ2dmO2aX;uJIF{e2G7 NXX5ZlFsT2y7Y3;n[Y4he3mwdHjhd4Uh[WO2aY\peJkhd2ZiSFXLNlk{KGOnbHzzMEBqdmirYnn0bY9vKG:oIFfTT|Mu[mW2YTygSWM2OD1yLkKg{txO M4D5R|EzQTRzM{Ox
human HDLM-2 cell MV7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MX3Jcohq[mm2aX;uJI9nKGi3bXHuJGhFVE1vMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNlA6QDRizszN NXfJRm5rW0GQR1XS
human NCI-H1770 cell MXzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYmy[G9qUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFE4PzBiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeT6gTWM2OD1yLkK5N|kzKM7:TR?= M2DY[HNCVkeHUh?=
human HOP-62 cell NWG0VZVmT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MojuTY5pcWKrdHnvckBw\iCqdX3hckBJV1BvNkKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlg2OjV4IN88US=> NXXZVHI4W0GQR1XS
human JAR cell NXrL[nU3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1;V[GlvcGmkaYTpc44hd2ZiaIXtZY4hUkGUIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT6yNVA1PCEQvF2= NEmzcYhUSU6JRWK=
human K5 cell MXrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXTJcohq[mm2aX;uJI9nKGi3bXHuJGs2KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImsJGlEPTB;MT61N|Y6QCEQvF2= NYfiZVRSW0GQR1XS
human MV-4-11 cell NXHrfWxoT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkfuTY5pcWKrdHnvckBw\iCqdX3hckBOXi12LUGxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Nk43QTZ6NTFOwG0> NWTrb45lW0GQR1XS
human BT-549 cell NUPPfXlqT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2TTNGlvcGmkaYTpc44hd2ZiaIXtZY4hSlRvNUS5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OU4zQTN5NjFOwG0> NEPTfYJUSU6JRWK=
human LB2241-RCC cell M1fBWWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MUnJcohq[mm2aX;uJI9nKGi3bXHuJGxDOjJ2MT3SR2Mh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF03NjR{OEe0JO69VQ>? MoK3V2FPT0WU
human GAMG cell M{\lbGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWPRWFl5UW6qaXLpeIlwdiCxZjDoeY1idiCJQV3HJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Ok43PTRyOTFOwG0> MXjTRW5ITVJ?
human HMV-II cell MmXrS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NG\2Z49KdmirYnn0bY9vKG:oIHj1cYFvKEiPVj3JTUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVcvPjd4MEeg{txO NITRcoNUSU6JRWK=
human RS4-11 cell M{fycGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWTJcohq[mm2aX;uJI9nKGi3bXHuJHJUPC1zMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUiuNlQyPDFizszN M2TKRnNCVkeHUh?=
human NCI-H1993 cell MV;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NFnvcoRKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INVk6OyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRThwM{[1N|Qh|ryP Mn2yV2FPT0WU
human IST-SL1 cell NWDGOYJLT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWDJcohq[mm2aX;uJI9nKGi3bXHuJGlUXC2VTEGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME25MlAzODRizszN MYDTRW5ITVJ?
human SK-OV-3 cell MojjS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NVrPZ405UW6qaXLpeIlwdiCxZjDoeY1idiCVSz3PWk0{KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:QS5zMECyJO69VQ>? MXXTRW5ITVJ?
human ESS-1 cell NWnrV|dpT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHvKSpBKdmirYnn0bY9vKG:oIHj1cYFvKEWVUz2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PU42QTh5MjFOwG0> M37nVnNCVkeHUh?=
human G-361 cell NVy1NldMT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2O0Z2lvcGmkaYTpc44hd2ZiaIXtZY4hTy1|NkGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNE4yPzl6IN88US=> M2LOenNCVkeHUh?=
human ALL-PO cell NITOZo1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MWLJcohq[mm2aX;uJI9nKGi3bXHuJGFNVC2STzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGwMlM1PzdizszN M2TaTnNCVkeHUh?=
human NB5 cell NWjFeZRlT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVzJcohq[mm2aX;uJI9nKGi3bXHuJG5DPSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFzLkKxOVch|ryP M2D0WnNCVkeHUh?=
human DU-145 cell NVHCboxkT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MnThTY5pcWKrdHnvckBw\iCqdX3hckBFXS1zNEWgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNU43PTN3IN88US=> MUXTRW5ITVJ?
human NMC-G1 cell MYLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXzRUXlbUW6qaXLpeIlwdiCxZjDoeY1idiCQTVOtS|Eh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOS55Mk[0JO69VQ>? M2jOTHNCVkeHUh?=
human RPMI-7951 cell M{ew[Gdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MV7Jcohq[mm2aX;uJI9nKGi3bXHuJHJRVUlvN{m1NUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NiCLQ{WwQVEyNjd6NkSg{txO NEHxVHVUSU6JRWK=
human NCI-H2009 cell NGHs[YNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4W4WGlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyNFA6KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTNwMkSyO{DPxE1? M3rYbnNCVkeHUh?=
human LoVo cell NXXvXpJsT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NY\hXHJlUW6qaXLpeIlwdiCxZjDoeY1idiCOb2\vJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVMvOzd5MTFOwG0> M3rpdXNCVkeHUh?=
human A2058 cell MVjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1fvRmlvcGmkaYTpc44hd2ZiaIXtZY4hSTJyNUigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{41PjlizszN MnTmV2FPT0WU
human D-566MG cell NGnEUlBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2S4bWlvcGmkaYTpc44hd2ZiaIXtZY4hTC13Nk\NS{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE{Njl2OEGg{txO MULTRW5ITVJ?
human QIMR-WIL cell NXuyPXBDT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYfWN49uUW6qaXLpeIlwdiCxZjDoeY1idiCTSV3SMXdKVCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTF3Lk[zPVQh|ryP MV3TRW5ITVJ?
human S-117 cell MWXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYrBd2ZZUW6qaXLpeIlwdiCxZjDoeY1idiCVLUGxO{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE3NjRyMkKg{txO NIj3TYxUSU6JRWK=
human YH-13 cell M4\wSmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MW\Jcohq[mm2aX;uJI9nKGi3bXHuJHlJNTF|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MU[uOlg3PSEQvF2= MnjJV2FPT0WU
human IGROV-1 cell NG\wUHRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MWnJcohq[mm2aX;uJI9nKGi3bXHuJGlIWk:YLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xO{4zODBzIN88US=> NWHYVnVsW0GQR1XS
human BHT-101 cell MV\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NF;0RlBKdmirYnn0bY9vKG:oIHj1cYFvKEKKVD2xNFEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yPy54MEm5JO69VQ>? NUH2SmtpW0GQR1XS
human MKN45 cell NYLyOZNGT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NETiXHhKdmirYnn0bY9vKG:oIHj1cYFvKE2NTkS1JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVgvPDF{ODFOwG0> NH;LSGdUSU6JRWK=
human A498 cell M1;PVGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MlS3TY5pcWKrdHnvckBw\iCqdX3hckBCPDl6IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUmuOFU1QSEQvF2= NUDpUmFsW0GQR1XS
human U-266 cell NUm1V5dtT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlvsTY5pcWKrdHnvckBw\iCqdX3hckBWNTJ4NjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKwMlg4QTdizszN NUTOS5Y5W0GQR1XS
human BFTC-905 cell NXHqW2t7T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3nUfGlvcGmkaYTpc44hd2ZiaIXtZY4hSk[WQz25NFUh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zOS5zOEe5JO69VQ>? NG\VU2tUSU6JRWK=
human BxPC-3 cell MkXYS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NFnldXVKdmirYnn0bY9vKG:oIHj1cYFvKEK6UFOtN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIyNjN3NUSg{txO MkPFV2FPT0WU
human NCI-SNU-1 cell NIXsWmRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MljOTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvU17VMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zOS53NUW4JO69VQ>? NFrsUHlUSU6JRWK=
human SF539 cell M1zkc2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2jGWmlvcGmkaYTpc44hd2ZiaIXtZY4hW0Z3M{mgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yNk4xOTl6IN88US=> M2DCPHNCVkeHUh?=
human VA-ES-BJ cell NFnuV4tIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MknCTY5pcWKrdHnvckBw\iCqdX3hckBXSS2HUz3CTkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIzNjV5NkOg{txO MYfTRW5ITVJ?
human HuO-3N1 cell NIe0eXBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MX3Jcohq[mm2aX;uJI9nKGi3bXHuJGh2Vy1|TkGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yNk43OTVzIN88US=> MX;TRW5ITVJ?
human EM-2 cell NHzEcGRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGfJ[mFKdmirYnn0bY9vKG:oIHj1cYFvKEWPLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yNk43Pjh7IN88US=> MW\TRW5ITVJ?
human T98G cell M3PMZmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWnVd|BlUW6qaXLpeIlwdiCxZjDoeY1idiCWOUjHJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlMvOzByOTFOwG0> M1rvZ3NCVkeHUh?=
human SW1783 cell NXrCW|lDT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MX\Jcohq[mm2aX;uJI9nKGi3bXHuJHNYOTd6MzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKzMlUzPDNizszN MYLTRW5ITVJ?
human NKM-1 cell MVfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1fVOGlvcGmkaYTpc44hd2ZiaIXtZY4hVkuPLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yN{45PTF{IN88US=> NFe4fJdUSU6JRWK=
human KOSC-2 cell MkHtS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MX7Jcohq[mm2aX;uJI9nKGi3bXHuJGtQW0NvMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUK0MlEzQTJizszN M4PG[3NCVkeHUh?=
human SW48 cell MXXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXnT[Y9wUW6qaXLpeIlwdiCxZjDoeY1idiCVV{S4JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlQvPjB4NzFOwG0> MYDTRW5ITVJ?
human MIAPaCa2 cells M1Pybmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4HxWFczKGh? NX23ZmlsT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hVUmDUHHDZVIh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UV{Bie3OjeTygTWM2OD1{NTFOwG0> M2f2elE6OzN6M{W1
human NCI-H28 cell NIizV5ZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MU\Jcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMkigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yOU4zOTB2IN88US=> NVPXTWxFW0GQR1XS
human 5637 cell M{D4[mdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4X4VGlvcGmkaYTpc44hd2ZiaIXtZY4hPTZ|NzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUK1MlgyOTFizszN NHXwXFlUSU6JRWK=
human NB69 cell NXPWbI84T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYfJcohq[mm2aX;uJI9nKGi3bXHuJG5DPjliY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1{Nj6xO|Y1KM7:TR?= MXzTRW5ITVJ?
human HT-29 cell M2jZPWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M33Hb2lvcGmkaYTpc44hd2ZiaIXtZY4hUFRvMkmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yO{4yPzV{IN88US=> NIO3RmZUSU6JRWK=
human PFSK-1 cell NVvIeFNvT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVvmc2VyUW6qaXLpeIlwdiCxZjDoeY1idiCSRmPLMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zPy53OUOg{txO M37GfnNCVkeHUh?=
human UACC-257 cell NEXlNmdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1fTUWlvcGmkaYTpc44hd2ZiaIXtZY4hXUGFQz2yOVch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zPy55NEOg{txO MXjTRW5ITVJ?
human D-423MG cell NUHHWFBDT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1;SW2lvcGmkaYTpc44hd2ZiaIXtZY4hTC12MkPNS{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVI4NjlzME[g{txO M4jSS3NCVkeHUh?=
human NH-12 cell MorjS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXGybXVmUW6qaXLpeIlwdiCxZjDoeY1idiCQSD2xNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVI5NjRyNUmg{txO NHLUUmpUSU6JRWK=
human DEL cell NIjxS2VIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MmLtTY5pcWKrdHnvckBw\iCqdX3hckBFTUxiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1{OT6wOFc1KM7:TR?= NES2WItUSU6JRWK=
human LS-513 cell MX3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXHJcohq[mm2aX;uJI9nKGi3bXHuJGxUNTVzMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOwMlE{OzRizszN MoXHV2FPT0WU
human NBsusSR cell NWTNUJNXT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYHJcohq[mm2aX;uJI9nKGi3bXHuJG5De3W|U2KgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zNE42Pjd6IN88US=> M3vae3NCVkeHUh?=
human BV-173 cell M{PNSGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MnLrTY5pcWKrdHnvckBw\iCqdX3hckBDXi1zN{OgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zNE44PjR7IN88US=> Mmn3V2FPT0WU
human A101D cell NVrpUGZGT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NGW4XY1KdmirYnn0bY9vKG:oIHj1cYFvKEFzMEHEJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|AvQTd6NDFOwG0> NUiwO2hrW0GQR1XS
human LU-134-A cell NU\BPW9ET3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NEixXZBKdmirYnn0bY9vKG:oIHj1cYFvKEyXLUGzOE1CKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OzFwMEKzPEDPxE1? MX\TRW5ITVJ?
human ES3 cell NIXIc|FIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M{\wcGlvcGmkaYTpc44hd2ZiaIXtZY4hTVN|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;M{GuN|M4PiEQvF2= MYLTRW5ITVJ?
human NY cell MUHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYnJcohq[mm2aX;uJI9nKGi3bXHuJG5[KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OzJwMkm2OUDPxE1? M3XUWXNCVkeHUh?=
human NCI-H1975 cell NIHoNI1Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mn\WTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEG5O|Uh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{Oi5|NUiyJO69VQ>? NXrSd49YW0GQR1XS
human A704 cell NHuyXIxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlXNTY5pcWKrdHnvckBw\iCqdX3hckBCPzB2IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;M{SuNlA2QSEQvF2= MorCV2FPT0WU
human SK-MEL-24 cell NXzEZXl6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2jYVWlvcGmkaYTpc44hd2ZiaIXtZY4hW0tvTVXMMVI1KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OzRwMkWyN{DPxE1? MmPEV2FPT0WU
human SW1088 cell MUjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGj1TFZKdmirYnn0bY9vKG:oIHj1cYFvKFOZMUC4PEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVM1NjN2NUKg{txO MlXYV2FPT0WU
human HepG2 cells NX7BO5NCTnWwY4Tpc44h[XO|YYm= MVezOEDPxE1? NEPk[I0yOiCq M2PVfWlvcGmkaYTpc44hd2ZiR2PLN4JmfGFiaX6gbJVu[W5iSHXwS|Ih[2WubIOgZZN{\XO|ZXSgZZMh\GWlcnXhd4UhcW5icnH0bY8hd2ZicHjvd5Bpd3K7bHH0[YQhT1NxR2OgZZQhOzRidV2gZYZ1\XJiMUKgbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=> MXiyOVQ3PzF3MB?=
mouse 3T3L1 cells NEjwW|RHfW6ldHnvckBie3OjeR?= M2rpXVM1KM7:TR?= MkPrNVIhcA>? NITafIlKdmO{ZXHz[UBqdiCpbIXjc5NmKGmwY3;ydI9z[XSrb36gbY4hdW:3c3WgN3Q{VDFiY3XscJMh[XRiM{SgeW0h[W[2ZYKgNVIhcHK|IHL5JIdtfWOxc3WgZZN{[Xl? MYCyOVQ3PzF3MB?=
human ST14A cells M1;lZmZ2dmO2aX;uJIF{e2G7 MnzqOkBp NIDxfZNKdmirYnn0bY9vKG:oIFfTT{0{NWKndHGtcYVlcWG2ZXSgW451KHOrZ37hcIlv\yCrbjDoeY1idiCVVEG0RUBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCjY3P1cZVt[XSrb36gc4Yh[mW2YT3jZZNmcW5iYYLveY5lKG63Y3zleZMh[W[2ZYKgOkBpenNiYomgcYlkem:|Y3;wbYMh[W6jbInzbZM> M3\yTFIxPzB6OUO3
human ReNcell VM cells NF;NSnZRem:uaX\ldoF1cW:wIHHzd4F6 Mm[wN{DPxE1? M2\CTFczKGh? MVzBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFKnTnPlcIwhXk1iY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIH;mJINmdGxicILvcIln\XKjdHnvckBifCB|IIXNJIFnfGW{IEeyJIhzew>? MXmyNFcxQDl|Nx?=
human SK-MEL-1 cell NWPxWIhkT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NIjUUmZKdmirYnn0bY9vKG:oIHj1cYFvKFONLV3FUE0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImsJGlEPTB;M{SuO|E1KM7:TR?= MXjTRW5ITVJ?
human MOLT-4 cell MoH0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mo\YTY5pcWKrdHnvckBw\iCqdX3hckBOV0yWLUSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zOk4yPDZ5IN88US=> MU\TRW5ITVJ?
human T47D cell M2DiOWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mm[3TY5pcWKrdHnvckBw\iCqdX3hckBVPDeGIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;M{euNVY1PyEQvF2= M1Wwe3NCVkeHUh?=
human SW1710 cell NHqyOZpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVfJcohq[mm2aX;uJI9nKGi3bXHuJHNYOTdzMDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUO3MlM3ODhizszN MoPuV2FPT0WU
human MKN7 cell NXXk[Fc6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NGn0Z5lKdmirYnn0bY9vKG:oIHj1cYFvKE2NTkegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zPE4xQTB7IN88US=> MX3TRW5ITVJ?
human CAL-72 cell MVTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3W1[GlvcGmkaYTpc44hd2ZiaIXtZY4hS0GOLUeyJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|gvOTF5ODFOwG0> NHzqSYNUSU6JRWK=
human AGS cell NW\a[HUxT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWHCfmtIUW6qaXLpeIlwdiCxZjDoeY1idiCDR2OgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zPE4zODN7IN88US=> MoTjV2FPT0WU
human BE-13 cell Mmn4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Ml\aTY5pcWKrdHnvckBw\iCqdX3hckBDTS1zMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUO4Mlc4QDNizszN NW\nOG82W0GQR1XS
human Calu-6 cell NFXL[Y9Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlzzTY5pcWKrdHnvckBw\iCqdX3hckBE[Wy3LU[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zPU43QTZ2IN88US=> M2m5fHNCVkeHUh?=
human CAL-27 cell MkPVS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGW1Z|hKdmirYnn0bY9vKG:oIHj1cYFvKEODTD2yO{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQ1NjByNUSg{txO NVPWdWJVW0GQR1XS
human BB49-HNC cell NGHyO|lIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Ml;YTY5pcWKrdHnvckBw\iCqdX3hckBDSjR7LVjOR{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQ1NjB{N{ig{txO NUGzdHZRW0GQR1XS

... Click to View More Cell Line Experimental Data

In vivo Administration of SB 216763 at 20 mg/kg significantly prevents lung inflammation and the subsequent fibrosis in bleomycin (BLM)-induced pulmonary inflammation and fibrosis model in mice by significantly blocking the production of inflammatory cytokines MCP-1 and TNF-α by macrophages, and significantly improves the survival of BLM-treated mice. SB 216763 treatment causes a significant reduction in BLM-induced alveolitis by inhibiting alveolar epithelial cell damage. [4]

Protocol

Kinase Assay:[1]
+ Expand

GSK-3 activity assay:

GSK-3 kinase activity is measured, in the presence of various concentrations of SB 216763, in a reaction mixture containing final concentrations of 1 nM human GSK-3α, 50 mM MOPS pH 7.0, 0.2 mM EDTA, 10 mM Mg-acetate, 7.5 mM β-mercaptoethanol, 5% (w/v) glycerol, 0.01% (w/v) Tween-20, 10% (v/v) DMSO, and 28 μM GS-2 peptide substrate. The GS-2 peptide sequence corresponds to a region of glycogen synthase that is phosphorylated by GSK-3. The assay is initiated by the addition of 0.34 μCi [33P]γ-ATP. The total ATP concentration is 10 μM. Following 30 minutes incubation at room temperature the assay is stopped by the addition of one third assay volume of 2.5% (v/v) H3PO4 containing 21 mM ATP. Samples are spotted onto P30 phosphocellulose mats and washed six times in 0.5% (v/v) H3PO4. The filter mats are sealed into sample bags containing Wallac betaplate scintillation fluid. 33P incorporation into the substrate peptide is determined by counting the mats in a Wallac microbeta scintillation counter.
Cell Research:[3]
+ Expand
  • Cell lines: BXPC-3, MIA-PaCa2, PANC1, ASPC1, and CFPAC cells
  • Concentrations: Dissolved in DMSO, final concentrations ~50 μM
  • Incubation Time: 24, 48, and 72 hours
  • Method: Cells are exposed to various concentrations of SB 216763 for 24, 48 and 72 hours. Relative cell viability is measured using the MTS assay. Apoptotic cells are determined by staining with Hoechst.
    (Only for Reference)
Animal Research:[4]
+ Expand
  • Animal Models: C57BL/6N mice with lung inflammation and fibrosis induced by bleomycin (BLM)
  • Formulation: Dissolved in DMSO, and diluted in saline
  • Dosages: 20 mg/kg
  • Administration: Intravenously
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 23 mg/mL (61.95 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order:
5% DMSO+corn oil
For best results, use promptly after mixing.
4mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 371.22
Formula

C19H12N2O2Cl2

CAS No. 280744-09-4
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID