SB216763

Catalog No.S1075

SB216763 Chemical Structure

Molecular Weight(MW): 371.22

SB216763 is a potent and selective GSK-3 inhibitor with IC50 of 34.3 nM for GSK-3α and equally effective at inhibiting human GSK-3β.

Size Price Stock Quantity  
In DMSO USD 165 In stock
USD 147 In stock
USD 270 In stock
USD 870 In stock
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3 Customer Reviews

  • Mol Cancer Ther 2014 13(1), 37-48. SB216763 purchased from Selleck.

    Viability assays for five additional GSK3 inhibitors in SY5Y cells.

    Mol Cancer Ther, 2013, 13(2): 454-67 . SB216763 purchased from Selleck.

  • In the presence of SB216763 (2 nM), transwell migration and invasion assay was conducted to evaluate the cell metastasis after Serpine2 transfection. Data were from three independent experiments and were average ± S.E. values. **P < 0.01, compared to transfected vector cells; ##P < 0.01, compared to transfected Serpine2 cells.

    Am J Transl Res, 2016, 8(5):1985-97.. SB216763 purchased from Selleck.

Purity & Quality Control

Choose Selective GSK-3 Inhibitors

Biological Activity

Description SB216763 is a potent and selective GSK-3 inhibitor with IC50 of 34.3 nM for GSK-3α and equally effective at inhibiting human GSK-3β.
Targets
GSK-3α [1]
(Cell-free assay)
GSK-3β [1]
(Cell-free assay)
34.3 nM ~34.3 nM
In vitro

SB 216763 displays similar potency for GSK-3β with 96% inhibition at 10 μM while exhibiting minimal activity against 24 other protein kinases including PKBα and PDK1 with IC50 of >10 μM. SB 216763 stimulates glycogen synthesis in human liver cells with EC50 of 3.6 μM, and induces dose-dependent transcription of a β-catenin-LEF/TCF regulated reporter gene in HEK293 cells with a maximum 2.5-fold induction at 5 μM. [1] SB 216763 protects the cerebellar granule neurones from apoptotic cell death induced by LY-294002 or potassium-deprivation in a concentration-dependent manner, with a maximal neuroprotection at 3 μM in contrast with the effect of lithium chloride at which 10 mM is required. SB 216763 at 3 μM also completely prevents death of chicken dorsal root ganglion sensory neurones induced by LY-294002 regardless of NGF. SB 216763 treatment at 5 μM markedly inhibits the GSK-3-dependent phosphorylation of neuronal-specific microtubule-associated protein tau in cerebellar granule neurones or recombinant tau in HEK293 cells, and induces increased levels of cytoplasmic β-catenin in both cells mimicking the effect of Wnt-mediated inhibition of GSK-3. [2] In pancreatic cancer cell lines including BXPC-3, MIA-PaCa2, PANC1, ASPC1, and CFPAC, SB 216763 treatment at 25-50 μM reduces cell viability in a dose-dependent manner, and leads to significant increase in apoptosis by 50% at 72 hours due to the specific down regulation of GSK-3β, while has no effect in HMEC or WI38 cell lines. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEK293 cells MWXGeY5kfGmxbjDhd5NigQ>? Mli1S4x6[2:pZX6gd5lvfGijc3WgZYN1cX[rdImgc4YhUEWNMkmzJINmdGy|LDDpcohq[mm2aX;uJI9nKEeVS{OtZoV1[SxiRVO1NF0xNjJizszN MXexNlk1OTN|MR?=
human HDLM-2 cell NGnvSYtIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Ml7YTY5pcWKrdHnvckBw\iCqdX3hckBJTEyPLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlIxQTh2IN88US=> MXnTRW5ITVJ?
human NCI-H1770 cell M2HTPWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NX3ZZWMzUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFE4PzBiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeT6gTWM2OD1yLkK5N|kzKM7:TR?= MX7TRW5ITVJ?
human HOP-62 cell NFrXT5RIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4TseGlvcGmkaYTpc44hd2ZiaIXtZY4hUE:SLU[yJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE45PTJ3NjFOwG0> MmWxV2FPT0WU
human JAR cell MnTqS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MonjTY5pcWKrdHnvckBw\iCqdX3hckBLSVJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLkKxNFQ1KM7:TR?= NH7OOWNUSU6JRWK=
human K5 cell M2DQWWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MmrMTY5pcWKrdHnvckBw\iCqdX3hckBMPSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5MEBKSzVyPUGuOVM3QThizszN MX;TRW5ITVJ?
human MV-4-11 cell NV63dY9wT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{jjN2lvcGmkaYTpc44hd2ZiaIXtZY4hVVZvND2xNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIvPjl4OEWg{txO MYjTRW5ITVJ?
human BT-549 cell M3iwNGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXPySmJ7UW6qaXLpeIlwdiCxZjDoeY1idiCEVD21OFkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF02NjJ7M{e2JO69VQ>? MnW3V2FPT0WU
human LB2241-RCC cell MW\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXrJcohq[mm2aX;uJI9nKGi3bXHuJGxDOjJ2MT3SR2Mh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF03NjR{OEe0JO69VQ>? NHnhW5NUSU6JRWK=
human GAMG cell MoOzS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2XOUmlvcGmkaYTpc44hd2ZiaIXtZY4hT0GPRzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPU[uOlU1ODlizszN Mmq3V2FPT0WU
human HMV-II cell NUnTVWhrT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWfJcohq[mm2aX;uJI9nKGi3bXHuJGhOXi2LSTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUeuOlc3ODdizszN Mn;3V2FPT0WU
human RS4-11 cell MYrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGnJfIJKdmirYnn0bY9vKG:oIHj1cYFvKFKVND2xNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVgvOjRzNEGg{txO Ml3HV2FPT0WU
human NCI-H1993 cell MUDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MV7Jcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMUm5N{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVgvOzZ3M{Sg{txO MlfSV2FPT0WU
human IST-SL1 cell M{XIW2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MmnhTY5pcWKrdHnvckBw\iCqdX3hckBKW1RvU1yxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PU4xOjB2IN88US=> NFPLN2dUSU6JRWK=
human SK-OV-3 cell NGW5bHVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3npTGlvcGmkaYTpc44hd2ZiaIXtZY4hW0tvT2[tN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVkvOTByMjFOwG0> NHH2cZlUSU6JRWK=
human ESS-1 cell NH7p[|NIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYD6WlBWUW6qaXLpeIlwdiCxZjDoeY1idiCHU2OtNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVkvPTl6N{Kg{txO MXnTRW5ITVJ?
human G-361 cell MmLUS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlH1TY5pcWKrdHnvckBw\iCqdX3hckBINTN4MTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGwMlE4QThizszN NVrYVI1DW0GQR1XS
human ALL-PO cell NX3QSmRHT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2n6[2lvcGmkaYTpc44hd2ZiaIXtZY4hSUyOLWDPJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVAvOzR5NzFOwG0> MXPTRW5ITVJ?
human NB5 cell NHXORnVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2fVcmlvcGmkaYTpc44hd2ZiaIXtZY4hVkJ3IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGuNlE2PyEQvF2= MX\TRW5ITVJ?
human DU-145 cell MmLLS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3nt[GlvcGmkaYTpc44hd2ZiaIXtZY4hTFVvMUS1JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVEvPjV|NTFOwG0> MUTTRW5ITVJ?
human NMC-G1 cell M32ycGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWD1ZZJSUW6qaXLpeIlwdiCxZjDoeY1idiCQTVOtS|Eh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOS55Mk[0JO69VQ>? NG\lNGZUSU6JRWK=
human RPMI-7951 cell NV;aeoRYT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3;OUWlvcGmkaYTpc44hd2ZiaIXtZY4hWlCPST23PVUyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XlwIFnDOVA:OTFwN{i2OEDPxE1? NUfvUYhHW0GQR1XS
human NCI-H2009 cell NV\mdnl[T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{S3TmlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyNFA6KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTNwMkSyO{DPxE1? NXvyToNlW0GQR1XS
human LoVo cell M{nmbGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2jm[WlvcGmkaYTpc44hd2ZiaIXtZY4hVG:YbzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGzMlM4PzFizszN MV7TRW5ITVJ?
human A2058 cell MXfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVrJcohq[mm2aX;uJI9nKGi3bXHuJGEzODV6IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUOuOFY6KM7:TR?= NUXWVnQxW0GQR1XS
human D-566MG cell NIDJR3VIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MV7Jcohq[mm2aX;uJI9nKGi3bXHuJGQuPTZ4TVegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{46PDhzIN88US=> MXTTRW5ITVJ?
human QIMR-WIL cell MmXPS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVTJcohq[mm2aX;uJI9nKGi3bXHuJHFKVVJvV1nMJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVUvPjN7NDFOwG0> NVHZXJZ[W0GQR1XS
human S-117 cell M{jifGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXm5NIZSUW6qaXLpeIlwdiCxZjDoeY1idiCVLUGxO{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE3NjRyMkKg{txO NFexfXdUSU6JRWK=
human YH-13 cell NHjjV5RIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MWXJcohq[mm2aX;uJI9nKGi3bXHuJHlJNTF|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MU[uOlg3PSEQvF2= MnL1V2FPT0WU
human IGROV-1 cell NEHKbWJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= Mn3RTY5pcWKrdHnvckBw\iCqdX3hckBKT1KRVj2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVcvOjByMTFOwG0> M4PKRnNCVkeHUh?=
human BHT-101 cell NGi0WXVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2Lz[2lvcGmkaYTpc44hd2ZiaIXtZY4hSkiWLUGwNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE4NjZyOUmg{txO MVHTRW5ITVJ?
human MKN45 cell NHnCRmdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2XHPWlvcGmkaYTpc44hd2ZiaIXtZY4hVUuQNEWgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xPE41OTJ6IN88US=> MnX4V2FPT0WU
human A498 cell NGjEeYJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHm2W3dKdmirYnn0bY9vKG:oIHj1cYFvKEF2OUigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xPU41PTR7IN88US=> NUj0XI1MW0GQR1XS
human U-266 cell Mn3WS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVrJcohq[mm2aX;uJI9nKGi3bXHuJHUuOjZ4IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkCuPFc6PyEQvF2= Ml\KV2FPT0WU
human BFTC-905 cell M4DQb2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MoXnTY5pcWKrdHnvckBw\iCqdX3hckBDTlSFLUmwOUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIyNjF6N{mg{txO NFrvSJhUSU6JRWK=
human BxPC-3 cell NUjxVpdIT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{XTU2lvcGmkaYTpc44hd2ZiaIXtZY4hSniSQz2zJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlEvOzV3NDFOwG0> M{mzdHNCVkeHUh?=
human NCI-SNU-1 cell MY\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NFLoWopKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3TUnUuOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTJzLkW1OVgh|ryP NHTpcIZUSU6JRWK=
human SF539 cell NYnGNFJYT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlrSTY5pcWKrdHnvckBw\iCqdX3hckBUTjV|OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKyMlAyQThizszN MXfTRW5ITVJ?
human VA-ES-BJ cell MlryS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MkjCTY5pcWKrdHnvckBw\iCqdX3hckBXSS2HUz3CTkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIzNjV5NkOg{txO MkjBV2FPT0WU
human HuO-3N1 cell Mmm0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXjJcohq[mm2aX;uJI9nKGi3bXHuJGh2Vy1|TkGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yNk43OTVzIN88US=> NX7RV3JvW0GQR1XS
human EM-2 cell MV7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYPJcohq[mm2aX;uJI9nKGi3bXHuJGVONTJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1{Mj62Olg6KM7:TR?= MUPTRW5ITVJ?
human T98G cell MoLrS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUS1cW1XUW6qaXLpeIlwdiCxZjDoeY1idiCWOUjHJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlMvOzByOTFOwG0> NIToOFZUSU6JRWK=
human SW1783 cell M3\INmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1S3eWlvcGmkaYTpc44hd2ZiaIXtZY4hW1dzN{izJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlMvPTJ2MzFOwG0> MlX1V2FPT0WU
human NKM-1 cell M1nhS2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFr2[JVKdmirYnn0bY9vKG:oIHj1cYFvKE6NTT2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlMvQDVzMjFOwG0> MVrTRW5ITVJ?
human KOSC-2 cell M4rIXmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MoLjTY5pcWKrdHnvckBw\iCqdX3hckBMV1OFLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yOE4yOjl{IN88US=> MW\TRW5ITVJ?
human SW48 cell Mkn3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1j0bGlvcGmkaYTpc44hd2ZiaIXtZY4hW1d2ODDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUK0MlYxPjdizszN MUXTRW5ITVJ?
human MIAPaCa2 cells M2iyS2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4HKdFczKGh? NXewUI5vT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hVUmDUHHDZVIh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UV{Bie3OjeTygTWM2OD1{NTFOwG0> NVrOfmd[OTl|M{izOVU>
human NCI-H28 cell NGTNSmhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVHQOlFjUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFI5KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OjVwMkGwOEDPxE1? Ml;rV2FPT0WU
human 5637 cell M1XFSGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3WxdGlvcGmkaYTpc44hd2ZiaIXtZY4hPTZ|NzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUK1MlgyOTFizszN MYTTRW5ITVJ?
human NB69 cell NIW4fXRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXnmSGxrUW6qaXLpeIlwdiCxZjDoeY1idiCQQk[5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlYvOTd4NDFOwG0> NWPMdmVzW0GQR1XS
human HT-29 cell NUjqPHpLT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mm\XTY5pcWKrdHnvckBw\iCqdX3hckBJXC1{OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUK3MlE4PTJizszN M2fXPXNCVkeHUh?=
human PFSK-1 cell NFHmWlVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlX0TY5pcWKrdHnvckBw\iCqdX3hckBRTlONLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yO{42QTNizszN MnXRV2FPT0WU
human UACC-257 cell NW\STIF3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4r0W2lvcGmkaYTpc44hd2ZiaIXtZY4hXUGFQz2yOVch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zPy55NEOg{txO NYLzZm9UW0GQR1XS
human D-423MG cell M1\OOGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYDQeJJmUW6qaXLpeIlwdiCxZjDoeY1idiCGLUSyN21IKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OjdwOUGwOkDPxE1? NVL5WmlGW0GQR1XS
human NH-12 cell MnHDS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MWPJcohq[mm2aX;uJI9nKGi3bXHuJG5JNTF{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkiuOFA2QSEQvF2= MmKyV2FPT0WU
human DEL cell M{Hke2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2TRS2lvcGmkaYTpc44hd2ZiaIXtZY4hTEWOIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkmuNFQ4PCEQvF2= NVTPTHl2W0GQR1XS
human LS-513 cell NFrCXYxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NF3UNo9KdmirYnn0bY9vKG:oIHj1cYFvKEyVLUWxN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMxNjF|M{Sg{txO NFexOnJUSU6JRWK=
human NBsusSR cell M3G1XGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NEfN[mdKdmirYnn0bY9vKG:oIHj1cYFvKE6Ec4XzV3Ih[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{OC53Nke4JO69VQ>? NE\NS5hUSU6JRWK=
human BV-173 cell Ml[2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHy2WJZKdmirYnn0bY9vKG:oIHj1cYFvKEKYLUG3N{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMxNjd4NEmg{txO MmjCV2FPT0WU
human A101D cell MoP0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NIXZ[3lKdmirYnn0bY9vKG:oIHj1cYFvKEFzMEHEJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|AvQTd6NDFOwG0> MYrTRW5ITVJ?
human LU-134-A cell MVPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NG\ofGNKdmirYnn0bY9vKG:oIHj1cYFvKEyXLUGzOE1CKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OzFwMEKzPEDPxE1? NYDpNotoW0GQR1XS
human ES3 cell MXXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnHHTY5pcWKrdHnvckBw\iCqdX3hckBGWzNiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|MT6zN|c3KM7:TR?= NGTM[llUSU6JRWK=
human NY cell MlnrS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MoL6TY5pcWKrdHnvckBw\iCqdX3hckBPYSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTN{LkK5OlUh|ryP NILNVodUSU6JRWK=
human NCI-H1975 cell NVr4coRmT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MknxTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEG5O|Uh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{Oi5|NUiyJO69VQ>? M{WzRXNCVkeHUh?=
human A704 cell M{i5Tmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2PyRmlvcGmkaYTpc44hd2ZiaIXtZY4hSTdyNDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUO0MlIxPTlizszN MkjrV2FPT0WU
human SK-MEL-24 cell NUHrUYZiT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWn5VWV1UW6qaXLpeIlwdiCxZjDoeY1idiCVSz3NSWwuOjRiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|ND6yOVI{KM7:TR?= NGC0R5BUSU6JRWK=
human SW1088 cell NGH2bVBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUPqRXBrUW6qaXLpeIlwdiCxZjDoeY1idiCVV{GwPFgh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{PC5|NEWyJO69VQ>? NGHabXVUSU6JRWK=
human HepG2 cells MnLrSpVv[3Srb36gZZN{[Xl? NVP2ZmZiOzRizszN NEn3Z4QyOiCq Mm\NTY5pcWKrdHnvckBw\iCJU1uzZoV1[SCrbjDoeY1idiCKZYDHNkBk\WyuczDhd5Nme3OnZDDhd{Bl\WO{ZXHz[UBqdiC{YYTpc{Bw\iCyaH;zdIhwenmuYYTl[EBIWy:JUzDheEA{PCC3TTDh[pRmeiBzMjDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz NULGO3J6OjV2NkexOVA>
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human ST14A cells M3zaO2Z2dmO2aX;uJIF{e2G7 MUi2JIg> MV7Jcohq[mm2aX;uJI9nKEeVSz2zMYJmfGFvbXXkbYF1\WRiV370JJNq\26jbHnu[{BqdiCqdX3hckBUXDF2QTDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDpckBi[2O3bYXsZZRqd25ib3[gZoV1[S2lYYPlbY4h[XKxdX7kJI52[2yndYOgZYZ1\XJiNjDodpMh[nlibXnjdo9{[2:yaXOgZY5idHm|aYO= M3LEOFIxPzB6OUO3
human ReNcell VM cells MmfpVJJwdGmoZYLheIlwdiCjc4PhfS=> MknIN{DPxE1? MX[3NkBp NXv0e5liSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDS[W5k\WyuIG\NJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBw\iClZXzsJJBzd2yrZnXyZZRqd25iYYSgN{B2VSCjZoTldkA4OiCqcoO= MUeyNFcxQDl|Nx?=
human SK-MEL-1 cell M4C0W2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MV3Jcohq[mm2aX;uJI9nKGi3bXHuJHNMNU2HTD2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHluIFnDOVA:OzRwN{G0JO69VQ>? Ml7pV2FPT0WU
human MOLT-4 cell NFzZW4tIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NI\6UllKdmirYnn0bY9vKG:oIHj1cYFvKE2RTGStOEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVM3NjF2Nkeg{txO MnPmV2FPT0WU
human T47D cell M3T4Nmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3n6TmlvcGmkaYTpc44hd2ZiaIXtZY4hXDR5RDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUO3MlE3PDdizszN M4PVNHNCVkeHUh?=
human SW1710 cell MUfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MlTLTY5pcWKrdHnvckBw\iCqdX3hckBUXzF5MUCgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zO{4{PjB6IN88US=> MkHnV2FPT0WU
human MKN7 cell MWPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWHJcohq[mm2aX;uJI9nKGi3bXHuJG1MVjdiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|OD6wPVA6KM7:TR?= MXvTRW5ITVJ?
human CAL-72 cell NUjjZm56T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVfJcohq[mm2aX;uJI9nKGi3bXHuJGNCVC15MjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUO4MlEyPzhizszN NHvuUnJUSU6JRWK=
human AGS cell MlXlS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXnjeIFWUW6qaXLpeIlwdiCxZjDoeY1idiCDR2OgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zPE4zODN7IN88US=> NHrzO2NUSU6JRWK=
human BE-13 cell MYHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NEnWT5dKdmirYnn0bY9vKG:oIHj1cYFvKEKHLUGzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|gvPzd6MzFOwG0> NU\weph4W0GQR1XS
human Calu-6 cell M3v6Rmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MYPJcohq[mm2aX;uJI9nKGi3bXHuJGNidHVvNjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUO5MlY6PjRizszN MnPYV2FPT0WU
human CAL-27 cell NGPodIpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NX7OcYw6UW6qaXLpeIlwdiCxZjDoeY1idiCFQVytNlch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF01PC5yMEW0JO69VQ>? Mn73V2FPT0WU
human BB49-HNC cell MVzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXPJcohq[mm2aX;uJI9nKGi3bXHuJGJDPDlvSF7DJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OFQvODJ5ODFOwG0> M{nCXHNCVkeHUh?=

... Click to View More Cell Line Experimental Data

In vivo Administration of SB 216763 at 20 mg/kg significantly prevents lung inflammation and the subsequent fibrosis in bleomycin (BLM)-induced pulmonary inflammation and fibrosis model in mice by significantly blocking the production of inflammatory cytokines MCP-1 and TNF-α by macrophages, and significantly improves the survival of BLM-treated mice. SB 216763 treatment causes a significant reduction in BLM-induced alveolitis by inhibiting alveolar epithelial cell damage. [4]

Protocol

Kinase Assay:[1]
+ Expand

GSK-3 activity assay:

GSK-3 kinase activity is measured, in the presence of various concentrations of SB 216763, in a reaction mixture containing final concentrations of 1 nM human GSK-3α, 50 mM MOPS pH 7.0, 0.2 mM EDTA, 10 mM Mg-acetate, 7.5 mM β-mercaptoethanol, 5% (w/v) glycerol, 0.01% (w/v) Tween-20, 10% (v/v) DMSO, and 28 μM GS-2 peptide substrate. The GS-2 peptide sequence corresponds to a region of glycogen synthase that is phosphorylated by GSK-3. The assay is initiated by the addition of 0.34 μCi [33P]γ-ATP. The total ATP concentration is 10 μM. Following 30 minutes incubation at room temperature the assay is stopped by the addition of one third assay volume of 2.5% (v/v) H3PO4 containing 21 mM ATP. Samples are spotted onto P30 phosphocellulose mats and washed six times in 0.5% (v/v) H3PO4. The filter mats are sealed into sample bags containing Wallac betaplate scintillation fluid. 33P incorporation into the substrate peptide is determined by counting the mats in a Wallac microbeta scintillation counter.
Cell Research:[3]
+ Expand
  • Cell lines: BXPC-3, MIA-PaCa2, PANC1, ASPC1, and CFPAC cells
  • Concentrations: Dissolved in DMSO, final concentrations ~50 μM
  • Incubation Time: 24, 48, and 72 hours
  • Method: Cells are exposed to various concentrations of SB 216763 for 24, 48 and 72 hours. Relative cell viability is measured using the MTS assay. Apoptotic cells are determined by staining with Hoechst.
    (Only for Reference)
Animal Research:[4]
+ Expand
  • Animal Models: C57BL/6N mice with lung inflammation and fibrosis induced by bleomycin (BLM)
  • Formulation: Dissolved in DMSO, and diluted in saline
  • Dosages: 20 mg/kg
  • Administration: Intravenously
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 23 mg/mL (61.95 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents individually and in order:
5% DMSO+corn oil
4mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 371.22
Formula

C19H12N2O2Cl2

CAS No. 280744-09-4
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID