SB216763

Catalog No.S1075

SB216763 Chemical Structure

Molecular Weight(MW): 371.22

SB216763 is a potent and selective GSK-3 inhibitor with IC50 of 34.3 nM for GSK-3α and equally effective at inhibiting human GSK-3β.

Size Price Stock Quantity  
In DMSO USD 165 In stock
USD 147 In stock
USD 270 In stock
USD 870 In stock
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3 Customer Reviews

  • Mol Cancer Ther 2014 13(1), 37-48. SB216763 purchased from Selleck.

    Viability assays for five additional GSK3 inhibitors in SY5Y cells.

    Mol Cancer Ther, 2013, 13(2): 454-67 . SB216763 purchased from Selleck.

  • In the presence of SB216763 (2 nM), transwell migration and invasion assay was conducted to evaluate the cell metastasis after Serpine2 transfection. Data were from three independent experiments and were average ± S.E. values. **P < 0.01, compared to transfected vector cells; ##P < 0.01, compared to transfected Serpine2 cells.

    Am J Transl Res, 2016, 8(5):1985-97.. SB216763 purchased from Selleck.

Purity & Quality Control

Choose Selective GSK-3 Inhibitors

Biological Activity

Description SB216763 is a potent and selective GSK-3 inhibitor with IC50 of 34.3 nM for GSK-3α and equally effective at inhibiting human GSK-3β.
Targets
GSK-3α [1]
(Cell-free assay)
GSK-3β [1]
(Cell-free assay)
34.3 nM ~34.3 nM
In vitro

SB 216763 displays similar potency for GSK-3β with 96% inhibition at 10 μM while exhibiting minimal activity against 24 other protein kinases including PKBα and PDK1 with IC50 of >10 μM. SB 216763 stimulates glycogen synthesis in human liver cells with EC50 of 3.6 μM, and induces dose-dependent transcription of a β-catenin-LEF/TCF regulated reporter gene in HEK293 cells with a maximum 2.5-fold induction at 5 μM. [1] SB 216763 protects the cerebellar granule neurones from apoptotic cell death induced by LY-294002 or potassium-deprivation in a concentration-dependent manner, with a maximal neuroprotection at 3 μM in contrast with the effect of lithium chloride at which 10 mM is required. SB 216763 at 3 μM also completely prevents death of chicken dorsal root ganglion sensory neurones induced by LY-294002 regardless of NGF. SB 216763 treatment at 5 μM markedly inhibits the GSK-3-dependent phosphorylation of neuronal-specific microtubule-associated protein tau in cerebellar granule neurones or recombinant tau in HEK293 cells, and induces increased levels of cytoplasmic β-catenin in both cells mimicking the effect of Wnt-mediated inhibition of GSK-3. [2] In pancreatic cancer cell lines including BXPC-3, MIA-PaCa2, PANC1, ASPC1, and CFPAC, SB 216763 treatment at 25-50 μM reduces cell viability in a dose-dependent manner, and leads to significant increase in apoptosis by 50% at 72 hours due to the specific down regulation of GSK-3β, while has no effect in HMEC or WI38 cell lines. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEK293 cells MWTGeY5kfGmxbjDhd5NigQ>? MknES4x6[2:pZX6gd5lvfGijc3WgZYN1cX[rdImgc4YhUEWNMkmzJINmdGy|LDDpcohq[mm2aX;uJI9nKEeVS{OtZoV1[SxiRVO1NF0xNjJizszN M4fBSlEzQTRzM{Ox
human HDLM-2 cell MlOzS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYPJcohq[mm2aX;uJI9nKGi3bXHuJGhFVE1vMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuNlA6QDRizszN NULLT|RDW0GQR1XS
human NCI-H1770 cell NIDi[oFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVjJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMUe3NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NiCLQ{WwQVAvOjl|OUKg{txO M{ftdXNCVkeHUh?=
human HOP-62 cell NWqxbox6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MoTWTY5pcWKrdHnvckBw\iCqdX3hckBJV1BvNkKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlg2OjV4IN88US=> M1vNd3NCVkeHUh?=
human JAR cell NYLycId1T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MljYTY5pcWKrdHnvckBw\iCqdX3hckBLSVJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLkKxNFQ1KM7:TR?= NIrCcXdUSU6JRWK=
human K5 cell NV;TXng2T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NEPVe4VKdmirYnn0bY9vKG:oIHj1cYFvKEt3IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJktKEmFNUC9NU42OzZ7ODFOwG0> M1O3XnNCVkeHUh?=
human MV-4-11 cell MXPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWfnWoh1UW6qaXLpeIlwdiCxZjDoeY1idiCPVj20MVEyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Oi54OU[4OUDPxE1? M3rGVnNCVkeHUh?=
human BT-549 cell MYHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHzYWG1KdmirYnn0bY9vKG:oIHj1cYFvKEKWLUW0PUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVUvOjl|N{[g{txO M13DfnNCVkeHUh?=
human LB2241-RCC cell MlPWS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MoL5TY5pcWKrdHnvckBw\iCqdX3hckBNSjJ{NEGtVmNEKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:Pi52Mki3OEDPxE1? NIHHOGRUSU6JRWK=
human GAMG cell M4[2RWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVq0ToJHUW6qaXLpeIlwdiCxZjDoeY1idiCJQV3HJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Ok43PTRyOTFOwG0> NF7PTndUSU6JRWK=
human HMV-II cell M2HQVWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MorSTY5pcWKrdHnvckBw\iCqdX3hckBJVVZvSVmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23MlY4PjB5IN88US=> Mn3vV2FPT0WU
human RS4-11 cell NVLnelN3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NGnaWJJKdmirYnn0bY9vKG:oIHj1cYFvKFKVND2xNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVgvOjRzNEGg{txO MmX4V2FPT0WU
human NCI-H1993 cell MX\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXz6WFVkUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFE6QTNiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD16LkO2OVM1KM7:TR?= NYjzZmI6W0GQR1XS
human IST-SL1 cell MmjHS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHiw[YZKdmirYnn0bY9vKG:oIHj1cYFvKEmVVD3TUFEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF06NjB{MESg{txO MnXRV2FPT0WU
human SK-OV-3 cell NUPKXHpvT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NInNUpZKdmirYnn0bY9vKG:oIHj1cYFvKFONLV;WMVMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF06NjFyMEKg{txO NVe5S2JnW0GQR1XS
human ESS-1 cell NYTCemFnT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXvLe2ViUW6qaXLpeIlwdiCxZjDoeY1idiCHU2OtNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVkvPTl6N{Kg{txO MnqxV2FPT0WU
human G-361 cell M2HnTGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4j0bmlvcGmkaYTpc44hd2ZiaIXtZY4hTy1|NkGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNE4yPzl6IN88US=> MXLTRW5ITVJ?
human ALL-PO cell MoPDS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYfJcohq[mm2aX;uJI9nKGi3bXHuJGFNVC2STzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGwMlM1PzdizszN MkCxV2FPT0WU
human NB5 cell M17VNWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYexWldXUW6qaXLpeIlwdiCxZjDoeY1idiCQQkWgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNU4zOTV5IN88US=> NIPVSJlUSU6JRWK=
human DU-145 cell NFLKeWNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWW5blJvUW6qaXLpeIlwdiCxZjDoeY1idiCGVT2xOFUh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOS54NUO1JO69VQ>? MULTRW5ITVJ?
human NMC-G1 cell MXPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYHMeFc5UW6qaXLpeIlwdiCxZjDoeY1idiCQTVOtS|Eh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOS55Mk[0JO69VQ>? MWDTRW5ITVJ?
human RPMI-7951 cell M4fMbGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVLoW3FwUW6qaXLpeIlwdiCxZjDoeY1idiCUUF3JMVc6PTFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeT6gTWM2OD1zMT63PFY1KM7:TR?= NWfTWm5xW0GQR1XS
human NCI-H2009 cell NF;QNmhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlfoTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEKwNFkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOy5{NEK3JO69VQ>? MlfnV2FPT0WU
human LoVo cell M1q1T2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVHJcohq[mm2aX;uJI9nKGi3bXHuJGxwXm9iY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMz6zO|cyKM7:TR?= NGi2R25USU6JRWK=
human A2058 cell M2jGTmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NW[xdVlnUW6qaXLpeIlwdiCxZjDoeY1idiCDMkC1PEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE{NjR4OTFOwG0> MmrkV2FPT0WU
human D-566MG cell NHnGZ|ZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MorTTY5pcWKrdHnvckBw\iCqdX3hckBFNTV4Nl3HJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVMvQTR6MTFOwG0> M1POUHNCVkeHUh?=
human QIMR-WIL cell MYnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYHJcohq[mm2aX;uJI9nKGi3bXHuJHFKVVJvV1nMJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVUvPjN7NDFOwG0> M3\CdXNCVkeHUh?=
human S-117 cell NH72flVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NETnXYhKdmirYnn0bY9vKG:oIHj1cYFvKFNvMUG3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVYvPDB{MjFOwG0> MknuV2FPT0WU
human YH-13 cell M{PHT2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MW\Jcohq[mm2aX;uJI9nKGi3bXHuJHlJNTF|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MU[uOlg3PSEQvF2= NIfnZXNUSU6JRWK=
human IGROV-1 cell MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWq2TmZ4UW6qaXLpeIlwdiCxZjDoeY1idiCLR2LPWk0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTdwMkCwNUDPxE1? MnjZV2FPT0WU
human BHT-101 cell NHu5N25Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MoWzTY5pcWKrdHnvckBw\iCqdX3hckBDUFRvMUCxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVcvPjB7OTFOwG0> M{ixTnNCVkeHUh?=
human MKN45 cell MV3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWDSZVljUW6qaXLpeIlwdiCxZjDoeY1idiCPS160OUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE5NjRzMkig{txO NIDuSo9USU6JRWK=
human A498 cell MVTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MonnTY5pcWKrdHnvckBw\iCqdX3hckBCPDl6IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUmuOFU1QSEQvF2= NFrSeJRUSU6JRWK=
human U-266 cell NIqzTmpIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnXFTY5pcWKrdHnvckBw\iCqdX3hckBWNTJ4NjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKwMlg4QTdizszN M1rjeXNCVkeHUh?=
human BFTC-905 cell NGHDdphIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4HzU2lvcGmkaYTpc44hd2ZiaIXtZY4hSk[WQz25NFUh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zOS5zOEe5JO69VQ>? M{H5RnNCVkeHUh?=
human BxPC-3 cell MULHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUXnUJF4UW6qaXLpeIlwdiCxZjDoeY1idiCEeGDDMVMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zOS5|NUW0JO69VQ>? NX3VU5kyW0GQR1XS
human NCI-SNU-1 cell MVvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NFO2S|ZKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3TUnUuOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTJzLkW1OVgh|ryP MoThV2FPT0WU
human SF539 cell NVvuOVZlT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3PnOWlvcGmkaYTpc44hd2ZiaIXtZY4hW0Z3M{mgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yNk4xOTl6IN88US=> NH\QO4tUSU6JRWK=
human VA-ES-BJ cell M1Xs[2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIjFTIVKdmirYnn0bY9vKG:oIHj1cYFvKF[DLVXTMWJLKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OjJwNUe2N{DPxE1? NVOzR49vW0GQR1XS
human HuO-3N1 cell M36xcGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Ml7vTY5pcWKrdHnvckBw\iCqdX3hckBJfU9vM16xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlIvPjF3MTFOwG0> M{nFfXNCVkeHUh?=
human EM-2 cell MWjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYXJcohq[mm2aX;uJI9nKGi3bXHuJGVONTJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1{Mj62Olg6KM7:TR?= MWLTRW5ITVJ?
human T98G cell NUTETYZGT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1\wfmlvcGmkaYTpc44hd2ZiaIXtZY4hXDl6RzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKzMlMxODlizszN NELRZnZUSU6JRWK=
human SW1783 cell NWjQXWc1T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYPJcohq[mm2aX;uJI9nKGi3bXHuJHNYOTd6MzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKzMlUzPDNizszN NFryZ4hUSU6JRWK=
human NKM-1 cell Mn3pS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHHVTVRKdmirYnn0bY9vKG:oIHj1cYFvKE6NTT2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlMvQDVzMjFOwG0> M3jlXnNCVkeHUh?=
human KOSC-2 cell NFv5UG5Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYXVNYxxUW6qaXLpeIlwdiCxZjDoeY1idiCNT2PDMVIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zPC5zMkmyJO69VQ>? NV7yb5VIW0GQR1XS
human SW48 cell NG\oT|RIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWTVfIpXUW6qaXLpeIlwdiCxZjDoeY1idiCVV{S4JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlQvPjB4NzFOwG0> MlP3V2FPT0WU
human MIAPaCa2 cells MX\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2W5PFczKGh? NFHPOmVIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBOUUGSYVPhNkBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTTJIF{e2G7LDDJR|UxRTJ3IN88US=> MVyxPVM{QDN3NR?=
human NCI-H28 cell NFrMdXhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYf1eZI1UW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFI5KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OjVwMkGwOEDPxE1? NHztSlVUSU6JRWK=
human 5637 cell NGrSS|lIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXrzUYUzUW6qaXLpeIlwdiCxZjDoeY1idiB3NkO3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlUvQDFzMTFOwG0> MojWV2FPT0WU
human NB69 cell NGPET2tIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MoPVTY5pcWKrdHnvckBw\iCqdX3hckBPSjZ7IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mk[uNVc3PCEQvF2= NVvte4VVW0GQR1XS
human HT-29 cell NWDkdIxIT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NI\wSpZKdmirYnn0bY9vKG:oIHj1cYFvKEiWLUK5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlcvOTd3MjFOwG0> Mn;QV2FPT0WU
human PFSK-1 cell NY\NfHhLT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFrPUndKdmirYnn0bY9vKG:oIHj1cYFvKFCIU1utNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVI4NjV7MzFOwG0> M2jB[XNCVkeHUh?=
human UACC-257 cell NFzHOpVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVjTenhFUW6qaXLpeIlwdiCxZjDoeY1idiCXQVPDMVI2PyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTJ5Lke0N{DPxE1? MoXOV2FPT0WU
human D-423MG cell MmW0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmjwTY5pcWKrdHnvckBw\iCqdX3hckBFNTR{M13HJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlcvQTFyNjFOwG0> NEizUpZUSU6JRWK=
human NH-12 cell MUXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4nxWmlvcGmkaYTpc44hd2ZiaIXtZY4hVkhvMUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yPE41ODV7IN88US=> NXvOSFZGW0GQR1XS
human DEL cell NGTFUJBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NEP0S|BKdmirYnn0bY9vKG:oIHj1cYFvKESHTDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUK5MlA1PzRizszN M{ey[HNCVkeHUh?=
human LS-513 cell MkLJS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MX3Jcohq[mm2aX;uJI9nKGi3bXHuJGxUNTVzMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUOwMlE{OzRizszN MVjTRW5ITVJ?
human NBsusSR cell MnLOS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MoXYTY5pcWKrdHnvckBw\iCqdX3hckBPSnO3c2PSJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|AvPTZ5ODFOwG0> NGHpdXNUSU6JRWK=
human BV-173 cell M1rIc2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFvpbJdKdmirYnn0bY9vKG:oIHj1cYFvKEKYLUG3N{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMxNjd4NEmg{txO NGHkSIxUSU6JRWK=
human A101D cell NI\GWpFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4Hje2lvcGmkaYTpc44hd2ZiaIXtZY4hSTFyMVSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zNE46Pzh2IN88US=> NY\0XnlNW0GQR1XS
human LU-134-A cell MX;Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2XSRWlvcGmkaYTpc44hd2ZiaIXtZY4hVFVvMUO0MWEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{OS5yMkO4JO69VQ>? NVjlcINsW0GQR1XS
human ES3 cell NIDHc25Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1:2VmlvcGmkaYTpc44hd2ZiaIXtZY4hTVN|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;M{GuN|M4PiEQvF2= MYrTRW5ITVJ?
human NY cell M{nUU2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MlnSTY5pcWKrdHnvckBw\iCqdX3hckBPYSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTN{LkK5OlUh|ryP M3vvWnNCVkeHUh?=
human NCI-H1975 cell MkTzS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MkGyTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEG5O|Uh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{Oi5|NUiyJO69VQ>? MY\TRW5ITVJ?
human A704 cell M4f4[Gdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGTTVolKdmirYnn0bY9vKG:oIHj1cYFvKEF5MESgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zOE4zODV7IN88US=> M3T2O3NCVkeHUh?=
human SK-MEL-24 cell MlH0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NIXxUW9KdmirYnn0bY9vKG:oIHj1cYFvKFONLV3FUE0zPCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTN2LkK1NlMh|ryP NEf1c21USU6JRWK=
human SW1088 cell NV7R[VJUT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2L5SmlvcGmkaYTpc44hd2ZiaIXtZY4hW1dzMEi4JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|QvOzR3MjFOwG0> M37ITnNCVkeHUh?=
human HepG2 cells M2nRZmZ2dmO2aX;uJIF{e2G7 MmjFN|Qh|ryP MUexNkBp NImyUIRKdmirYnn0bY9vKG:oIFfTT|Nj\XSjIHnuJIh2dWGwIFjldGczKGOnbHzzJIF{e2W|c3XkJIF{KGSnY4LlZZNmKGmwIILheIlwKG:oIIDoc5NxcG:{eXzheIVlKEeVL1fTJIF1KDN2IIXNJIFnfGW{IEGyJIhzeyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM> NEPCfGEzPTR4N{G1NC=>
mouse 3T3L1 cells NVWxdmpOTnWwY4Tpc44h[XO|YYm= NX\HTYtXOzRizszN Mo\XNVIhcA>? NUnBRpM{UW6lcnXhd4UhcW5iZ3z1Z49{\SCrbnPvdpBwemG2aX;uJIlvKG2xdYPlJFNVO0xzIHPlcIx{KGG2IEO0JJVOKGGodHXyJFEzKGi{czDifUBodHWlb4PlJIF{e2G7 MoDsNlU1PjdzNUC=
human ST14A cells MVHGeY5kfGmxbjDhd5NigQ>? Ml\QOkBp NW[3TI12UW6qaXLpeIlwdiCxZjDHV2suOy2kZYThMY1m\GmjdHXkJHdvfCC|aXfuZYxqdmdiaX6gbJVu[W5iU2SxOGEh[2WubIOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iYXPjeY12dGG2aX;uJI9nKGKndHGtZ4F{\WmwIHHyc5Vv\CCwdXPs[ZV{KGGodHXyJFYhcHK|IHL5JI1q[3Kxc3PvdIlkKGGwYXz5d4l{ M1zWWFIxPzB6OUO3
human ReNcell VM cells NWXkSm5DWHKxbHnm[ZJifGmxbjDhd5NigQ>? NGjDOHE{KM7:TR?= MVS3NkBp NFv3[|FCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGLlUoNmdGxiVl2gZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJI9nKGOnbHygdJJwdGmoZYLheIlwdiCjdDCzJJVOKGGodHXyJFczKGi{cx?= MkL2NlA4ODh7M{e=
human SK-MEL-1 cell MXfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NFLiSYlKdmirYnn0bY9vKG:oIHj1cYFvKFONLV3FUE0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImsJGlEPTB;M{SuO|E1KM7:TR?= MU\TRW5ITVJ?
human MOLT-4 cell MVXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MX7Jcohq[mm2aX;uJI9nKGi3bXHuJG1QVFRvNDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUO2MlE1PjdizszN NGPSVFJUSU6JRWK=
human T47D cell MkLMS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXPJcohq[mm2aX;uJI9nKGi3bXHuJHQ1P0RiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|Nz6xOlQ4KM7:TR?= NGjSVFRUSU6JRWK=
human SW1710 cell MYTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3;WOmlvcGmkaYTpc44hd2ZiaIXtZY4hW1dzN{GwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|cvOzZyODFOwG0> NXrxPWlrW0GQR1XS
human MKN7 cell M3r2U2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MoPJTY5pcWKrdHnvckBw\iCqdX3hckBOU055IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;M{iuNFkxQSEQvF2= M4f5c3NCVkeHUh?=
human CAL-72 cell MV7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYH2PIVKUW6qaXLpeIlwdiCxZjDoeY1idiCFQVytO|Ih[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{QC5zMUe4JO69VQ>? NH6yO|BUSU6JRWK=
human AGS cell NUDCWZZwT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2XpbGlvcGmkaYTpc44hd2ZiaIXtZY4hSUeVIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;M{iuNlA{QSEQvF2= M{HUd3NCVkeHUh?=
human BE-13 cell NVLrc44zT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1H5XWlvcGmkaYTpc44hd2ZiaIXtZY4hSkVvMUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zPE44Pzh|IN88US=> NU\yfHEzW0GQR1XS
human Calu-6 cell NHLiRmRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlzkTY5pcWKrdHnvckBw\iCqdX3hckBE[Wy3LU[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zPU43QTZ2IN88US=> M{X4RnNCVkeHUh?=
human CAL-27 cell MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NE[1T2RKdmirYnn0bY9vKG:oIHj1cYFvKEODTD2yO{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQ1NjByNUSg{txO M4r4S3NCVkeHUh?=
human BB49-HNC cell NFPMe5dIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NX70SI5JUW6qaXLpeIlwdiCxZjDoeY1idiCEQkS5MWhPSyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTR2LkCyO|gh|ryP NXXMeppWW0GQR1XS

... Click to View More Cell Line Experimental Data

In vivo Administration of SB 216763 at 20 mg/kg significantly prevents lung inflammation and the subsequent fibrosis in bleomycin (BLM)-induced pulmonary inflammation and fibrosis model in mice by significantly blocking the production of inflammatory cytokines MCP-1 and TNF-α by macrophages, and significantly improves the survival of BLM-treated mice. SB 216763 treatment causes a significant reduction in BLM-induced alveolitis by inhibiting alveolar epithelial cell damage. [4]

Protocol

Kinase Assay:[1]
+ Expand

GSK-3 activity assay:

GSK-3 kinase activity is measured, in the presence of various concentrations of SB 216763, in a reaction mixture containing final concentrations of 1 nM human GSK-3α, 50 mM MOPS pH 7.0, 0.2 mM EDTA, 10 mM Mg-acetate, 7.5 mM β-mercaptoethanol, 5% (w/v) glycerol, 0.01% (w/v) Tween-20, 10% (v/v) DMSO, and 28 μM GS-2 peptide substrate. The GS-2 peptide sequence corresponds to a region of glycogen synthase that is phosphorylated by GSK-3. The assay is initiated by the addition of 0.34 μCi [33P]γ-ATP. The total ATP concentration is 10 μM. Following 30 minutes incubation at room temperature the assay is stopped by the addition of one third assay volume of 2.5% (v/v) H3PO4 containing 21 mM ATP. Samples are spotted onto P30 phosphocellulose mats and washed six times in 0.5% (v/v) H3PO4. The filter mats are sealed into sample bags containing Wallac betaplate scintillation fluid. 33P incorporation into the substrate peptide is determined by counting the mats in a Wallac microbeta scintillation counter.
Cell Research:[3]
+ Expand
  • Cell lines: BXPC-3, MIA-PaCa2, PANC1, ASPC1, and CFPAC cells
  • Concentrations: Dissolved in DMSO, final concentrations ~50 μM
  • Incubation Time: 24, 48, and 72 hours
  • Method: Cells are exposed to various concentrations of SB 216763 for 24, 48 and 72 hours. Relative cell viability is measured using the MTS assay. Apoptotic cells are determined by staining with Hoechst.
    (Only for Reference)
Animal Research:[4]
+ Expand
  • Animal Models: C57BL/6N mice with lung inflammation and fibrosis induced by bleomycin (BLM)
  • Formulation: Dissolved in DMSO, and diluted in saline
  • Dosages: 20 mg/kg
  • Administration: Intravenously
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 23 mg/mL (61.95 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order:
5% DMSO+corn oil
For best results, use promptly after mixing.
4mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 371.22
Formula

C19H12N2O2Cl2

CAS No. 280744-09-4
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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  • Computed Result

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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID