SB216763

Catalog No.S1075

SB216763 Chemical Structure

Molecular Weight(MW): 371.22

SB216763 is a potent and selective GSK-3 inhibitor with IC50 of 34.3 nM for GSK-3α and equally effective at inhibiting human GSK-3β.

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In DMSO USD 165 In stock
USD 147 In stock
USD 270 In stock
USD 870 In stock
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3 Customer Reviews

  • Mol Cancer Ther 2014 13(1), 37-48. SB216763 purchased from Selleck.

    Viability assays for five additional GSK3 inhibitors in SY5Y cells.

    Mol Cancer Ther, 2013, 13(2): 454-67 . SB216763 purchased from Selleck.

  • In the presence of SB216763 (2 nM), transwell migration and invasion assay was conducted to evaluate the cell metastasis after Serpine2 transfection. Data were from three independent experiments and were average ± S.E. values. **P < 0.01, compared to transfected vector cells; ##P < 0.01, compared to transfected Serpine2 cells.

    Am J Transl Res, 2016, 8(5):1985-97.. SB216763 purchased from Selleck.

Purity & Quality Control

Choose Selective GSK-3 Inhibitors

Biological Activity

Description SB216763 is a potent and selective GSK-3 inhibitor with IC50 of 34.3 nM for GSK-3α and equally effective at inhibiting human GSK-3β.
Targets
GSK-3α [1]
(Cell-free assay)
GSK-3β [1]
(Cell-free assay)
34.3 nM ~34.3 nM
In vitro

SB 216763 displays similar potency for GSK-3β with 96% inhibition at 10 μM while exhibiting minimal activity against 24 other protein kinases including PKBα and PDK1 with IC50 of >10 μM. SB 216763 stimulates glycogen synthesis in human liver cells with EC50 of 3.6 μM, and induces dose-dependent transcription of a β-catenin-LEF/TCF regulated reporter gene in HEK293 cells with a maximum 2.5-fold induction at 5 μM. [1] SB 216763 protects the cerebellar granule neurones from apoptotic cell death induced by LY-294002 or potassium-deprivation in a concentration-dependent manner, with a maximal neuroprotection at 3 μM in contrast with the effect of lithium chloride at which 10 mM is required. SB 216763 at 3 μM also completely prevents death of chicken dorsal root ganglion sensory neurones induced by LY-294002 regardless of NGF. SB 216763 treatment at 5 μM markedly inhibits the GSK-3-dependent phosphorylation of neuronal-specific microtubule-associated protein tau in cerebellar granule neurones or recombinant tau in HEK293 cells, and induces increased levels of cytoplasmic β-catenin in both cells mimicking the effect of Wnt-mediated inhibition of GSK-3. [2] In pancreatic cancer cell lines including BXPC-3, MIA-PaCa2, PANC1, ASPC1, and CFPAC, SB 216763 treatment at 25-50 μM reduces cell viability in a dose-dependent manner, and leads to significant increase in apoptosis by 50% at 72 hours due to the specific down regulation of GSK-3β, while has no effect in HMEC or WI38 cell lines. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEK293 cells MkTXSpVv[3Srb36gZZN{[Xl? MkTIS4x6[2:pZX6gd5lvfGijc3WgZYN1cX[rdImgc4YhUEWNMkmzJINmdGy|LDDpcohq[mm2aX;uJI9nKEeVS{OtZoV1[SxiRVO1NF0xNjJizszN MnvCNVI6PDF|M{G=
human HDLM-2 cell NITzZo9Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NY\TRYFRUW6qaXLpeIlwdiCxZjDoeY1idiCKRFzNMVIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjJyOUi0JO69VQ>? MV3TRW5ITVJ?
human NCI-H1770 cell NVjGeIxPT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWHJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMUe3NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NiCLQ{WwQVAvOjl|OUKg{txO NEHONnlUSU6JRWK=
human HOP-62 cell MkG0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Ml3qTY5pcWKrdHnvckBw\iCqdX3hckBJV1BvNkKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlg2OjV4IN88US=> NILXO41USU6JRWK=
human JAR cell MWDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MmC3TY5pcWKrdHnvckBw\iCqdX3hckBLSVJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLkKxNFQ1KM7:TR?= MkTDV2FPT0WU
human K5 cell NHftbmNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHfE[|BKdmirYnn0bY9vKG:oIHj1cYFvKEt3IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJktKEmFNUC9NU42OzZ7ODFOwG0> M1S1WHNCVkeHUh?=
human MV-4-11 cell MXHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NE\wRYZKdmirYnn0bY9vKG:oIHj1cYFvKE2YLUStNVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjZ7Nki1JO69VQ>? NFLSZmZUSU6JRWK=
human BT-549 cell MoD1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MoXFTY5pcWKrdHnvckBw\iCqdX3hckBDXC13NEmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME21MlI6Ozd4IN88US=> M4rpXnNCVkeHUh?=
human LB2241-RCC cell MkjoS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NFrVRWxKdmirYnn0bY9vKG:oIHj1cYFvKEyEMkK0NU1TS0NiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD14LkSyPFc1KM7:TR?= MUfTRW5ITVJ?
human GAMG cell NXXVN|M5T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NX:zWld4UW6qaXLpeIlwdiCxZjDoeY1idiCJQV3HJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Ok43PTRyOTFOwG0> M3;xcHNCVkeHUh?=
human HMV-II cell NXjoWVVVT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MmK1TY5pcWKrdHnvckBw\iCqdX3hckBJVVZvSVmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23MlY4PjB5IN88US=> Mlj6V2FPT0WU
human RS4-11 cell Mn\OS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHXT[lZKdmirYnn0bY9vKG:oIHj1cYFvKFKVND2xNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVgvOjRzNEGg{txO M{e0e3NCVkeHUh?=
human NCI-H1993 cell NXPtXFVuT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NIHIWlVKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INVk6OyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRThwM{[1N|Qh|ryP NHjtT29USU6JRWK=
human IST-SL1 cell NEP0RmJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVzWNZd6UW6qaXLpeIlwdiCxZjDoeY1idiCLU2StV2wyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:QS5yMkC0JO69VQ>? MoTjV2FPT0WU
human SK-OV-3 cell M2O1c2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MUXJcohq[mm2aX;uJI9nKGi3bXHuJHNMNU:YLUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME25MlExODJizszN NV7SSFVrW0GQR1XS
human ESS-1 cell MVvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXTJcohq[mm2aX;uJI9nKGi3bXHuJGVUWy1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OT61PVg4OiEQvF2= NVnXVmxyW0GQR1XS
human G-361 cell MVLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mn31TY5pcWKrdHnvckBw\iCqdX3hckBINTN4MTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGwMlE4QThizszN M{nHO3NCVkeHUh?=
human ALL-PO cell NFz0e5RIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkXXTY5pcWKrdHnvckBw\iCqdX3hckBCVExvUF:gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNE4{PDd5IN88US=> Mo\iV2FPT0WU
human NB5 cell MkP2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4LDSWlvcGmkaYTpc44hd2ZiaIXtZY4hVkJ3IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGuNlE2PyEQvF2= NWfHW5pXW0GQR1XS
human DU-145 cell NILIb5NIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlXNTY5pcWKrdHnvckBw\iCqdX3hckBFXS1zNEWgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNU43PTN3IN88US=> NFi2ZnRUSU6JRWK=
human NMC-G1 cell M1vyVWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MX7Jcohq[mm2aX;uJI9nKGi3bXHuJG5OSy2JMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGxMlczPjRizszN NYrQZVhoW0GQR1XS
human RPMI-7951 cell NYD2eoRRT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlrDTY5pcWKrdHnvckBw\iCqdX3hckBTWE2LLUe5OVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigS5iSVO1NF0yOS55OE[0JO69VQ>? MmjYV2FPT0WU
human NCI-H2009 cell MYjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXzMNFkyUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFIxODliY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMz6yOFI4KM7:TR?= M1rDO3NCVkeHUh?=
human LoVo cell MULHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUDiXnZoUW6qaXLpeIlwdiCxZjDoeY1idiCOb2\vJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVMvOzd5MTFOwG0> MWLTRW5ITVJ?
human A2058 cell NGDhNXFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MYDJcohq[mm2aX;uJI9nKGi3bXHuJGEzODV6IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUOuOFY6KM7:TR?= M4jGVHNCVkeHUh?=
human D-566MG cell Mm\BS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MUTJcohq[mm2aX;uJI9nKGi3bXHuJGQuPTZ4TVegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{46PDhzIN88US=> NIW5WpZUSU6JRWK=
human QIMR-WIL cell NWK3XGRtT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXu1O3lsUW6qaXLpeIlwdiCxZjDoeY1idiCTSV3SMXdKVCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTF3Lk[zPVQh|ryP MVjTRW5ITVJ?
human S-117 cell M1fs[Gdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NF\DRWFKdmirYnn0bY9vKG:oIHj1cYFvKFNvMUG3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVYvPDB{MjFOwG0> MWDTRW5ITVJ?
human YH-13 cell NV\GZ|dPT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1PuUmlvcGmkaYTpc44hd2ZiaIXtZY4hYUhvMUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xOk43QDZ3IN88US=> MlHhV2FPT0WU
human IGROV-1 cell M2XEZ2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWLJcohq[mm2aX;uJI9nKGi3bXHuJGlIWk:YLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xO{4zODBzIN88US=> NHr4XZdUSU6JRWK=
human BHT-101 cell MYDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVLOXXo3UW6qaXLpeIlwdiCxZjDoeY1idiCESGStNVAyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTdwNkC5PUDPxE1? M163V3NCVkeHUh?=
human MKN45 cell MVTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVrPUm1tUW6qaXLpeIlwdiCxZjDoeY1idiCPS160OUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE5NjRzMkig{txO NUniSm9xW0GQR1XS
human A498 cell NVHvdVRiT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYLJcohq[mm2aX;uJI9nKGi3bXHuJGE1QThiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zOT60OVQ6KM7:TR?= NV;6Zlg5W0GQR1XS
human U-266 cell NXXxS|JFT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXHJcohq[mm2aX;uJI9nKGi3bXHuJHUuOjZ4IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkCuPFc6PyEQvF2= MWPTRW5ITVJ?
human BFTC-905 cell MV3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NX20d5pIUW6qaXLpeIlwdiCxZjDoeY1idiCERmTDMVkxPSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTJzLkG4O|kh|ryP M33pc3NCVkeHUh?=
human BxPC-3 cell MnTIS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGX4Z4pKdmirYnn0bY9vKG:oIHj1cYFvKEK6UFOtN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIyNjN3NUSg{txO NVfXOWFHW0GQR1XS
human NCI-SNU-1 cell MVzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHm1VnpKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3TUnUuOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTJzLkW1OVgh|ryP NFrGcIZUSU6JRWK=
human SF539 cell MoGwS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MWLJcohq[mm2aX;uJI9nKGi3bXHuJHNHPTN7IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkKuNFE6QCEQvF2= MojqV2FPT0WU
human VA-ES-BJ cell NITCbnhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkDjTY5pcWKrdHnvckBw\iCqdX3hckBXSS2HUz3CTkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIzNjV5NkOg{txO MVjTRW5ITVJ?
human HuO-3N1 cell M{jFPGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVfodJF3UW6qaXLpeIlwdiCxZjDoeY1idiCKdV:tN24yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OjJwNkG1NUDPxE1? MljyV2FPT0WU
human EM-2 cell M17SbWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mlz0TY5pcWKrdHnvckBw\iCqdX3hckBGVS1{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkKuOlY5QSEQvF2= M4DuXXNCVkeHUh?=
human T98G cell NG\PfpNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2HwZWlvcGmkaYTpc44hd2ZiaIXtZY4hXDl6RzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKzMlMxODlizszN MXjTRW5ITVJ?
human SW1783 cell MkXBS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MkW2TY5pcWKrdHnvckBw\iCqdX3hckBUXzF5OEOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yN{42OjR|IN88US=> MW\TRW5ITVJ?
human NKM-1 cell Ml3MS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MXHJcohq[mm2aX;uJI9nKGi3bXHuJG5MVS1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkOuPFUyOiEQvF2= MlfOV2FPT0WU
human KOSC-2 cell M2DZOmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 Mn;0TY5pcWKrdHnvckBw\iCqdX3hckBMV1OFLUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yOE4yOjl{IN88US=> M1;5TnNCVkeHUh?=
human SW48 cell NYWxW3Z7T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHfjO|RKdmirYnn0bY9vKG:oIHj1cYFvKFOZNEigZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yOE43ODZ5IN88US=> NUTDUFZ5W0GQR1XS
human MIAPaCa2 cells NVLOd49UT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFHRTpI4OiCq NYLpOGJPT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hVUmDUHHDZVIh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UV{Bie3OjeTygTWM2OD1{NTFOwG0> NHjPOlAyQTN|OEO1OS=>
human NCI-H28 cell MWTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NF;kfGJKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INlgh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zPS5{MUC0JO69VQ>? MnXvV2FPT0WU
human 5637 cell NUfFN|d6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NHTLZo5KdmirYnn0bY9vKG:oIHj1cYFvKDV4M{egZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yOU45OTFzIN88US=> MmOyV2FPT0WU
human NB69 cell MkG2S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1fESWlvcGmkaYTpc44hd2ZiaIXtZY4hVkJ4OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUK2MlE4PjRizszN MlL3V2FPT0WU
human HT-29 cell M3\nbGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MnnQTY5pcWKrdHnvckBw\iCqdX3hckBJXC1{OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUK3MlE4PTJizszN NELWUYdUSU6JRWK=
human PFSK-1 cell MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NX;ZR|AyUW6qaXLpeIlwdiCxZjDoeY1idiCSRmPLMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zPy53OUOg{txO MX;TRW5ITVJ?
human UACC-257 cell NEDoPWtIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3rn[GlvcGmkaYTpc44hd2ZiaIXtZY4hXUGFQz2yOVch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zPy55NEOg{txO MXHTRW5ITVJ?
human D-423MG cell MmXoS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Ml7HTY5pcWKrdHnvckBw\iCqdX3hckBFNTR{M13HJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlcvQTFyNjFOwG0> M1eycnNCVkeHUh?=
human NH-12 cell NVX2RnBiT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXXJcohq[mm2aX;uJI9nKGi3bXHuJG5JNTF{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkiuOFA2QSEQvF2= NUjOUo5XW0GQR1XS
human DEL cell NX\KPVRGT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NY\uXVFyUW6qaXLpeIlwdiCxZjDoeY1idiCGRVygZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yPU4xPDd2IN88US=> MlXvV2FPT0WU
human LS-513 cell MWPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NX;3U2g3UW6qaXLpeIlwdiCxZjDoeY1idiCOUz21NVMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{OC5zM{O0JO69VQ>? M1\rWnNCVkeHUh?=
human NBsusSR cell M{XIeGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MlTGTY5pcWKrdHnvckBw\iCqdX3hckBPSnO3c2PSJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|AvPTZ5ODFOwG0> MXvTRW5ITVJ?
human BV-173 cell NV3HNZNGT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2rLUmlvcGmkaYTpc44hd2ZiaIXtZY4hSlZvMUezJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|AvPzZ2OTFOwG0> NHG3ZnBUSU6JRWK=
human A101D cell MoDGS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MX3Jcohq[mm2aX;uJI9nKGi3bXHuJGEyODGGIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;M{CuPVc5PCEQvF2= MWrTRW5ITVJ?
human LU-134-A cell MXrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NH;1[lRKdmirYnn0bY9vKG:oIHj1cYFvKEyXLUGzOE1CKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OzFwMEKzPEDPxE1? M1L6VXNCVkeHUh?=
human ES3 cell NGHHWpJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MmL0TY5pcWKrdHnvckBw\iCqdX3hckBGWzNiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|MT6zN|c3KM7:TR?= MUTTRW5ITVJ?
human NY cell MljzS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MU\Jcohq[mm2aX;uJI9nKGi3bXHuJG5[KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OzJwMkm2OUDPxE1? NVLPSZRQW0GQR1XS
human NCI-H1975 cell NYDPNppQT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYjXR5VLUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFE6PzViY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|Mj6zOVgzKM7:TR?= NFm0e2RUSU6JRWK=
human A704 cell MmD6S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NFjlSo9KdmirYnn0bY9vKG:oIHj1cYFvKEF5MESgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zOE4zODV7IN88US=> Ml;5V2FPT0WU
human SK-MEL-24 cell NFXPdVRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1XSbWlvcGmkaYTpc44hd2ZiaIXtZY4hW0tvTVXMMVI1KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OzRwMkWyN{DPxE1? M{PoZXNCVkeHUh?=
human SW1088 cell NVHnU2hmT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NGezUZFKdmirYnn0bY9vKG:oIHj1cYFvKFOZMUC4PEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVM1NjN2NUKg{txO NGPsfFRUSU6JRWK=
human HepG2 cells NXXJdo9QTnWwY4Tpc44h[XO|YYm= M{XuXFM1KM7:TR?= MX[xNkBp NFrCenBKdmirYnn0bY9vKG:oIFfTT|Nj\XSjIHnuJIh2dWGwIFjldGczKGOnbHzzJIF{e2W|c3XkJIF{KGSnY4LlZZNmKGmwIILheIlwKG:oIIDoc5NxcG:{eXzheIVlKEeVL1fTJIF1KDN2IIXNJIFnfGW{IEGyJIhzeyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM> MYGyOVQ3PzF3MB?=
mouse 3T3L1 cells NFvoVoxHfW6ldHnvckBie3OjeR?= MnzWN|Qh|ryP M{\uN|EzKGh? NHLxTHhKdmO{ZXHz[UBqdiCpbIXjc5NmKGmwY3;ydI9z[XSrb36gbY4hdW:3c3WgN3Q{VDFiY3XscJMh[XRiM{SgeW0h[W[2ZYKgNVIhcHK|IHL5JIdtfWOxc3WgZZN{[Xl? MX:yOVQ3PzF3MB?=
human ST14A cells NISzZYRHfW6ldHnvckBie3OjeR?= MkDjOkBp MVLJcohq[mm2aX;uJI9nKEeVSz2zMYJmfGFvbXXkbYF1\WRiV370JJNq\26jbHnu[{BqdiCqdX3hckBUXDF2QTDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDpckBi[2O3bYXsZZRqd25ib3[gZoV1[S2lYYPlbY4h[XKxdX7kJI52[2yndYOgZYZ1\XJiNjDodpMh[nlibXnjdo9{[2:yaXOgZY5idHm|aYO= MW[yNFcxQDl|Nx?=
human ReNcell VM cells NGDBNGpRem:uaX\ldoF1cW:wIHHzd4F6 NFHadYo{KM7:TR?= NELkPIs4OiCq MX3BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFKnTnPlcIwhXk1iY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIH;mJINmdGxicILvcIln\XKjdHnvckBifCB|IIXNJIFnfGW{IEeyJIhzew>? MUSyNFcxQDl|Nx?=
human SK-MEL-1 cell M3niOGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFnVTIVKdmirYnn0bY9vKG:oIHj1cYFvKFONLV3FUE0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImsJGlEPTB;M{SuO|E1KM7:TR?= M3vxUnNCVkeHUh?=
human MOLT-4 cell NXX2RZhST3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mn3TTY5pcWKrdHnvckBw\iCqdX3hckBOV0yWLUSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zOk4yPDZ5IN88US=> NHnV[FdUSU6JRWK=
human T47D cell M1\BTWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1vSS2lvcGmkaYTpc44hd2ZiaIXtZY4hXDR5RDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUO3MlE3PDdizszN NYnjWW1rW0GQR1XS
human SW1710 cell NYnNNIFqT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MnrjTY5pcWKrdHnvckBw\iCqdX3hckBUXzF5MUCgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zO{4{PjB6IN88US=> NGr3bIdUSU6JRWK=
human MKN7 cell NEPoZYJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXi4ZnFHUW6qaXLpeIlwdiCxZjDoeY1idiCPS163JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|gvODlyOTFOwG0> MkG2V2FPT0WU
human CAL-72 cell NVuzfVEzT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{XBcmlvcGmkaYTpc44hd2ZiaIXtZY4hS0GOLUeyJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|gvOTF5ODFOwG0> NWrGdlhLW0GQR1XS
human AGS cell NH;zWFFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYPBdoFKUW6qaXLpeIlwdiCxZjDoeY1idiCDR2OgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zPE4zODN7IN88US=> NUfXfpFOW0GQR1XS
human BE-13 cell M{PxW2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{Lr[2lvcGmkaYTpc44hd2ZiaIXtZY4hSkVvMUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zPE44Pzh|IN88US=> NFnvNGRUSU6JRWK=
human Calu-6 cell MXjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MUnJcohq[mm2aX;uJI9nKGi3bXHuJGNidHVvNjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUO5MlY6PjRizszN NUPvWnZPW0GQR1XS
human CAL-27 cell MnXNS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MY\Jcohq[mm2aX;uJI9nKGi3bXHuJGNCVC1{NzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUS0MlAxPTRizszN NYTnfZJIW0GQR1XS
human BB49-HNC cell NXXJbYlGT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4X6ZmlvcGmkaYTpc44hd2ZiaIXtZY4hSkJ2OT3IUmMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF01PC5yMke4JO69VQ>? NGW4[nlUSU6JRWK=

... Click to View More Cell Line Experimental Data

In vivo Administration of SB 216763 at 20 mg/kg significantly prevents lung inflammation and the subsequent fibrosis in bleomycin (BLM)-induced pulmonary inflammation and fibrosis model in mice by significantly blocking the production of inflammatory cytokines MCP-1 and TNF-α by macrophages, and significantly improves the survival of BLM-treated mice. SB 216763 treatment causes a significant reduction in BLM-induced alveolitis by inhibiting alveolar epithelial cell damage. [4]

Protocol

Kinase Assay:[1]
+ Expand

GSK-3 activity assay:

GSK-3 kinase activity is measured, in the presence of various concentrations of SB 216763, in a reaction mixture containing final concentrations of 1 nM human GSK-3α, 50 mM MOPS pH 7.0, 0.2 mM EDTA, 10 mM Mg-acetate, 7.5 mM β-mercaptoethanol, 5% (w/v) glycerol, 0.01% (w/v) Tween-20, 10% (v/v) DMSO, and 28 μM GS-2 peptide substrate. The GS-2 peptide sequence corresponds to a region of glycogen synthase that is phosphorylated by GSK-3. The assay is initiated by the addition of 0.34 μCi [33P]γ-ATP. The total ATP concentration is 10 μM. Following 30 minutes incubation at room temperature the assay is stopped by the addition of one third assay volume of 2.5% (v/v) H3PO4 containing 21 mM ATP. Samples are spotted onto P30 phosphocellulose mats and washed six times in 0.5% (v/v) H3PO4. The filter mats are sealed into sample bags containing Wallac betaplate scintillation fluid. 33P incorporation into the substrate peptide is determined by counting the mats in a Wallac microbeta scintillation counter.
Cell Research:[3]
+ Expand
  • Cell lines: BXPC-3, MIA-PaCa2, PANC1, ASPC1, and CFPAC cells
  • Concentrations: Dissolved in DMSO, final concentrations ~50 μM
  • Incubation Time: 24, 48, and 72 hours
  • Method: Cells are exposed to various concentrations of SB 216763 for 24, 48 and 72 hours. Relative cell viability is measured using the MTS assay. Apoptotic cells are determined by staining with Hoechst.
    (Only for Reference)
Animal Research:[4]
+ Expand
  • Animal Models: C57BL/6N mice with lung inflammation and fibrosis induced by bleomycin (BLM)
  • Formulation: Dissolved in DMSO, and diluted in saline
  • Dosages: 20 mg/kg
  • Administration: Intravenously
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 23 mg/mL (61.95 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents individually and in order:
5% DMSO+corn oil
4mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 371.22
Formula

C19H12N2O2Cl2

CAS No. 280744-09-4
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID