SB216763

Catalog No.S1075

SB216763 Chemical Structure

Molecular Weight(MW): 371.22

SB216763 is a potent and selective GSK-3 inhibitor with IC50 of 34.3 nM for GSK-3α and equally effective at inhibiting human GSK-3β.

Size Price Stock Quantity  
In DMSO USD 165 In stock
USD 147 In stock
USD 270 In stock
USD 870 In stock
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3 Customer Reviews

  • Mol Cancer Ther 2014 13(1), 37-48. SB216763 purchased from Selleck.

    Viability assays for five additional GSK3 inhibitors in SY5Y cells.

    Mol Cancer Ther, 2013, 13(2): 454-67 . SB216763 purchased from Selleck.

  • In the presence of SB216763 (2 nM), transwell migration and invasion assay was conducted to evaluate the cell metastasis after Serpine2 transfection. Data were from three independent experiments and were average ± S.E. values. **P < 0.01, compared to transfected vector cells; ##P < 0.01, compared to transfected Serpine2 cells.

    Am J Transl Res, 2016, 8(5):1985-97.. SB216763 purchased from Selleck.

Purity & Quality Control

Choose Selective GSK-3 Inhibitors

Biological Activity

Description SB216763 is a potent and selective GSK-3 inhibitor with IC50 of 34.3 nM for GSK-3α and equally effective at inhibiting human GSK-3β.
Targets
GSK-3α [1]
(Cell-free assay)
GSK-3β [1]
(Cell-free assay)
34.3 nM ~34.3 nM
In vitro

SB 216763 displays similar potency for GSK-3β with 96% inhibition at 10 μM while exhibiting minimal activity against 24 other protein kinases including PKBα and PDK1 with IC50 of >10 μM. SB 216763 stimulates glycogen synthesis in human liver cells with EC50 of 3.6 μM, and induces dose-dependent transcription of a β-catenin-LEF/TCF regulated reporter gene in HEK293 cells with a maximum 2.5-fold induction at 5 μM. [1] SB 216763 protects the cerebellar granule neurones from apoptotic cell death induced by LY-294002 or potassium-deprivation in a concentration-dependent manner, with a maximal neuroprotection at 3 μM in contrast with the effect of lithium chloride at which 10 mM is required. SB 216763 at 3 μM also completely prevents death of chicken dorsal root ganglion sensory neurones induced by LY-294002 regardless of NGF. SB 216763 treatment at 5 μM markedly inhibits the GSK-3-dependent phosphorylation of neuronal-specific microtubule-associated protein tau in cerebellar granule neurones or recombinant tau in HEK293 cells, and induces increased levels of cytoplasmic β-catenin in both cells mimicking the effect of Wnt-mediated inhibition of GSK-3. [2] In pancreatic cancer cell lines including BXPC-3, MIA-PaCa2, PANC1, ASPC1, and CFPAC, SB 216763 treatment at 25-50 μM reduces cell viability in a dose-dependent manner, and leads to significant increase in apoptosis by 50% at 72 hours due to the specific down regulation of GSK-3β, while has no effect in HMEC or WI38 cell lines. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEK293 cells NHKxcFVHfW6ldHnvckBie3OjeR?= NYfZTpF1T2y7Y3;n[Y4he3mwdHjhd4Uh[WO2aY\peJkhd2ZiSFXLNlk{KGOnbHzzMEBqdmirYnn0bY9vKG:oIFfTT|Mu[mW2YTygSWM2OD1yLkKg{txO MlH3NVI6PDF|M{G=
human HDLM-2 cell NEe0Nm5Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NEL2Z4hKdmirYnn0bY9vKG:oIHj1cYFvKEiGTF2tNkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvOjB7OESg{txO NVi3Rnc6W0GQR1XS
human NCI-H1770 cell M2S3WWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NF2zT2hKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INVc4OCClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LjDJR|UxRTBwMkmzPVIh|ryP MlHUV2FPT0WU
human HOP-62 cell NF33RYZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVPJcohq[mm2aX;uJI9nKGi3bXHuJGhQWC14MjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuPFUzPTZizszN NYHib5hmW0GQR1XS
human JAR cell M2HhR2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4K4ZmlvcGmkaYTpc44hd2ZiaIXtZY4hUkGUIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT6yNVA1PCEQvF2= Mn3TV2FPT0WU
human K5 cell MmXpS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1zPWmlvcGmkaYTpc44hd2ZiaIXtZY4hUzViY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUwhUUN3ME2xMlU{Pjl6IN88US=> NEDKbFRUSU6JRWK=
human MV-4-11 cell M1z5dmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWHJcohq[mm2aX;uJI9nKGi3bXHuJG1XNTRvMUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlY6Pjh3IN88US=> MUPTRW5ITVJ?
human BT-549 cell MV3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYPJcohq[mm2aX;uJI9nKGi3bXHuJGJVNTV2OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuNlk{PzZizszN MVnTRW5ITVJ?
human LB2241-RCC cell M3ThVGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NI\SXWtKdmirYnn0bY9vKG:oIHj1cYFvKEyEMkK0NU1TS0NiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD14LkSyPFc1KM7:TR?= MmS4V2FPT0WU
human GAMG cell M3mxemdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWGweZZ3UW6qaXLpeIlwdiCxZjDoeY1idiCJQV3HJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9Ok43PTRyOTFOwG0> M{XtNXNCVkeHUh?=
human HMV-II cell M37RR2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGP2WnlKdmirYnn0bY9vKG:oIHj1cYFvKEiPVj3JTUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVcvPjd4MEeg{txO MmXZV2FPT0WU
human RS4-11 cell NF3ZcldIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUnLV|VKUW6qaXLpeIlwdiCxZjDoeY1idiCUU{StNVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF05NjJ2MUSxJO69VQ>? NUOxdlJLW0GQR1XS
human NCI-H1993 cell M2LSVWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MkHlTY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEG5PVMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF05NjN4NUO0JO69VQ>? NYrPUVJGW0GQR1XS
human IST-SL1 cell NEC4SopIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHf1XnNKdmirYnn0bY9vKG:oIHj1cYFvKEmVVD3TUFEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF06NjB{MESg{txO M2Pi[XNCVkeHUh?=
human SK-OV-3 cell Mn;HS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYHJcohq[mm2aX;uJI9nKGi3bXHuJHNMNU:YLUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME25MlExODJizszN MoqwV2FPT0WU
human ESS-1 cell NYDSV2dtT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1rRVWlvcGmkaYTpc44hd2ZiaIXtZY4hTVOVLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME25MlU6QDd{IN88US=> Mlr4V2FPT0WU
human G-361 cell NH7rXpFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NVjaS5lvUW6qaXLpeIlwdiCxZjDoeY1idiCJLUO2NUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVExNjF5OUig{txO M3Wy[HNCVkeHUh?=
human ALL-PO cell NHTHXGFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MkHsTY5pcWKrdHnvckBw\iCqdX3hckBCVExvUF:gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNE4{PDd5IN88US=> MkPHV2FPT0WU
human NB5 cell NXX0ToNpT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NH\kfZZKdmirYnn0bY9vKG:oIHj1cYFvKE6ENTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGxMlIyPTdizszN MXLTRW5ITVJ?
human DU-145 cell NFLrTlRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MX7Jcohq[mm2aX;uJI9nKGi3bXHuJGRWNTF2NTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGxMlY2OzVizszN MXLTRW5ITVJ?
human NMC-G1 cell MXPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWnnRWMxUW6qaXLpeIlwdiCxZjDoeY1idiCQTVOtS|Eh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOS55Mk[0JO69VQ>? MmTDV2FPT0WU
human RPMI-7951 cell M17HOmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MX3Jcohq[mm2aX;uJI9nKGi3bXHuJHJRVUlvN{m1NUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NiCLQ{WwQVEyNjd6NkSg{txO M3zScHNCVkeHUh?=
human NCI-H2009 cell NX7LOHB7T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYDJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2KMkCwPUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE{NjJ2Mkeg{txO Moi4V2FPT0WU
human LoVo cell NYrFTpFJT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NF3RUVRKdmirYnn0bY9vKG:oIHj1cYFvKEyxVn:gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xN{4{PzdzIN88US=> MXHTRW5ITVJ?
human A2058 cell NF[1WVFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MX\Jcohq[mm2aX;uJI9nKGi3bXHuJGEzODV6IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUOuOFY6KM7:TR?= MWfTRW5ITVJ?
human D-566MG cell Mki0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWn0fVZpUW6qaXLpeIlwdiCxZjDoeY1idiCGLUW2Om1IKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTNwOUS4NUDPxE1? MoDGV2FPT0WU
human QIMR-WIL cell NHfLT4ZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NHfMfpRKdmirYnn0bY9vKG:oIHj1cYFvKFGLTWKtW2lNKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTVwNkO5OEDPxE1? NUH6OmVPW0GQR1XS
human S-117 cell M3;6T2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWrIdGt{UW6qaXLpeIlwdiCxZjDoeY1idiCVLUGxO{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE3NjRyMkKg{txO MlnxV2FPT0WU
human YH-13 cell MlvaS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3fS[GlvcGmkaYTpc44hd2ZiaIXtZY4hYUhvMUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xOk43QDZ3IN88US=> MlqyV2FPT0WU
human IGROV-1 cell MkTBS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mn74TY5pcWKrdHnvckBw\iCqdX3hckBKT1KRVj2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVcvOjByMTFOwG0> NF;DeZZUSU6JRWK=
human BHT-101 cell MULHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M{fDbmlvcGmkaYTpc44hd2ZiaIXtZY4hSkiWLUGwNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVE4NjZyOUmg{txO NXLSVnVxW0GQR1XS
human MKN45 cell NYLWWHVlT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFrac2NKdmirYnn0bY9vKG:oIHj1cYFvKE2NTkS1JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVgvPDF{ODFOwG0> NGD2PZhUSU6JRWK=
human A498 cell MlXnS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVTJcohq[mm2aX;uJI9nKGi3bXHuJGE1QThiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zOT60OVQ6KM7:TR?= M1n0XnNCVkeHUh?=
human U-266 cell MYTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MmC3TY5pcWKrdHnvckBw\iCqdX3hckBWNTJ4NjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKwMlg4QTdizszN NHfCNGZUSU6JRWK=
human BFTC-905 cell NYexTIZtT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2\LdGlvcGmkaYTpc44hd2ZiaIXtZY4hSk[WQz25NFUh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zOS5zOEe5JO69VQ>? MorQV2FPT0WU
human BxPC-3 cell MYLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4i1OGlvcGmkaYTpc44hd2ZiaIXtZY4hSniSQz2zJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlEvOzV3NDFOwG0> Mmm4V2FPT0WU
human NCI-SNU-1 cell NX\1bmhrT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWfJcohq[mm2aX;uJI9nKGi3bXHuJG5EUS2VTmWtNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVIyNjV3NUig{txO MojTV2FPT0WU
human SF539 cell MWHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnjNTY5pcWKrdHnvckBw\iCqdX3hckBUTjV|OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKyMlAyQThizszN NG\CT5VUSU6JRWK=
human VA-ES-BJ cell M4L4[Wdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFjMdY9KdmirYnn0bY9vKG:oIHj1cYFvKF[DLVXTMWJLKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OjJwNUe2N{DPxE1? MXHTRW5ITVJ?
human HuO-3N1 cell MYTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVPJcohq[mm2aX;uJI9nKGi3bXHuJGh2Vy1|TkGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yNk43OTVzIN88US=> MVjTRW5ITVJ?
human EM-2 cell MUnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MmfMTY5pcWKrdHnvckBw\iCqdX3hckBGVS1{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkKuOlY5QSEQvF2= NUC5R3kxW0GQR1XS
human T98G cell MWfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3nNd2lvcGmkaYTpc44hd2ZiaIXtZY4hXDl6RzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKzMlMxODlizszN MXnTRW5ITVJ?
human SW1783 cell MWnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUfQT5p6UW6qaXLpeIlwdiCxZjDoeY1idiCVV{G3PFMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zOy53MkSzJO69VQ>? M1LmdXNCVkeHUh?=
human NKM-1 cell Mlu0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mmr6TY5pcWKrdHnvckBw\iCqdX3hckBPU01vMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKzMlg2OTJizszN MoqzV2FPT0WU
human KOSC-2 cell MVjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M{DiNWlvcGmkaYTpc44hd2ZiaIXtZY4hU0:VQz2yJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlQvOTJ7MjFOwG0> MlnTV2FPT0WU
human SW48 cell NXzUcHlLT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MV7Jcohq[mm2aX;uJI9nKGi3bXHuJHNYPDhiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1{ND62NFY4KM7:TR?= M{DmTXNCVkeHUh?=
human MIAPaCa2 cells NHHncJhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NIP2RWE4OiCq M3jsRmdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJG1KSVCjQ3GyJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGOgZZN{[XluIFnDOVA:OjVizszN NV7xVYd[OTl|M{izOVU>
human NCI-H28 cell MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1fmPWlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyPEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVI2NjJzMESg{txO NXfV[npqW0GQR1XS
human 5637 cell MnnUS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3m1W2lvcGmkaYTpc44hd2ZiaIXtZY4hPTZ|NzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUK1MlgyOTFizszN MVPTRW5ITVJ?
human NB69 cell MmrNS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MljhTY5pcWKrdHnvckBw\iCqdX3hckBPSjZ7IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Mk[uNVc3PCEQvF2= M2Doc3NCVkeHUh?=
human HT-29 cell NWjCXlRrT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NE[xPWRKdmirYnn0bY9vKG:oIHj1cYFvKEiWLUK5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlcvOTd3MjFOwG0> MVrTRW5ITVJ?
human PFSK-1 cell M2LzT2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIW2UFhKdmirYnn0bY9vKG:oIHj1cYFvKFCIU1utNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVI4NjV7MzFOwG0> NYi2OZFCW0GQR1XS
human UACC-257 cell MYPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGDxdI1KdmirYnn0bY9vKG:oIHj1cYFvKFWDQ1OtNlU4KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OjdwN{SzJO69VQ>? NFfr[W9USU6JRWK=
human D-423MG cell MYXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MkfOTY5pcWKrdHnvckBw\iCqdX3hckBFNTR{M13HJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlcvQTFyNjFOwG0> NWO1ZWJWW0GQR1XS
human NH-12 cell NUjvXHA2T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NEG1SYFKdmirYnn0bY9vKG:oIHj1cYFvKE6KLUGyJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlgvPDB3OTFOwG0> MWHTRW5ITVJ?
human DEL cell MWrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M{DGdmlvcGmkaYTpc44hd2ZiaIXtZY4hTEWOIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MkmuNFQ4PCEQvF2= NInnVIZUSU6JRWK=
human LS-513 cell M4LzcGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGX1NZlKdmirYnn0bY9vKG:oIHj1cYFvKEyVLUWxN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMxNjF|M{Sg{txO NIPUZ45USU6JRWK=
human NBsusSR cell Ml\JS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M4nWTmlvcGmkaYTpc44hd2ZiaIXtZY4hVkK|dYPTVkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMxNjV4N{ig{txO MUPTRW5ITVJ?
human BV-173 cell MoHGS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mle2TY5pcWKrdHnvckBw\iCqdX3hckBDXi1zN{OgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zNE44PjR7IN88US=> NF7JUHJUSU6JRWK=
human A101D cell MVjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NUXaeHpsUW6qaXLpeIlwdiCxZjDoeY1idiCDMUCxSEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMxNjl5OESg{txO MXLTRW5ITVJ?
human LU-134-A cell NYmyS3RHT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWnJcohq[mm2aX;uJI9nKGi3bXHuJGxWNTF|ND3BJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|EvODJ|ODFOwG0> MoXmV2FPT0WU
human ES3 cell NFe2O|NIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MX7Jcohq[mm2aX;uJI9nKGi3bXHuJGVUOyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTNzLkOzO|Yh|ryP NEDXSWNUSU6JRWK=
human NY cell M{LFVmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIrQVoJKdmirYnn0bY9vKG:oIHj1cYFvKE6\IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;M{KuNlk3PSEQvF2= M37aNXNCVkeHUh?=
human NCI-H1975 cell M3zKd2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NF\ibmhKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INVk4PSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTN{LkO1PFIh|ryP M{fRV3NCVkeHUh?=
human A704 cell Mkn3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUHGbHpIUW6qaXLpeIlwdiCxZjDoeY1idiCDN{C0JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|QvOjB3OTFOwG0> NV6yV2lwW0GQR1XS
human SK-MEL-24 cell M4nubWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NUDsRYdmUW6qaXLpeIlwdiCxZjDoeY1idiCVSz3NSWwuOjRiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|ND6yOVI{KM7:TR?= NFzNNIlUSU6JRWK=
human SW1088 cell NIO4ZY9Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2GwT2lvcGmkaYTpc44hd2ZiaIXtZY4hW1dzMEi4JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|QvOzR3MjFOwG0> NYT2eoZDW0GQR1XS
human HepG2 cells NXLiPY5JTnWwY4Tpc44h[XO|YYm= NHz6bmg{PCEQvF2= NX3PTpoyOTJiaB?= NWDpZXlwUW6qaXLpeIlwdiCxZjDHV2s{[mW2YTDpckBpfW2jbjDI[ZBIOiClZXzsd{Bie3Onc4Pl[EBieyCmZXPy[YF{\SCrbjDyZZRqdyCxZjDwbI9{eGixconsZZRm\CCJUz;HV{BifCB|NDD1UUBi\nSncjCxNkBpenNiYomgW4V{fGW{bjDicI91KGGwYXz5d4l{ MWmyOVQ3PzF3MB?=
mouse 3T3L1 cells MYjGeY5kfGmxbjDhd5NigQ>? MXKzOEDPxE1? NUDkUZR2OTJiaB?= MnTITY5kemWjc3WgbY4h\2y3Y3;z[UBqdmOxcoDvdoF1cW:wIHnuJI1wfXOnIEPUN2wyKGOnbHzzJIF1KDN2IIXNJIFnfGW{IEGyJIhzeyCkeTDncJVkd3OnIHHzd4F6 MomzNlU1PjdzNUC=
human ST14A cells NV71c3BwTnWwY4Tpc44h[XO|YYm= NVja[mx7PiCq MXfJcohq[mm2aX;uJI9nKEeVSz2zMYJmfGFvbXXkbYF1\WRiV370JJNq\26jbHnu[{BqdiCqdX3hckBUXDF2QTDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDpckBi[2O3bYXsZZRqd25ib3[gZoV1[S2lYYPlbY4h[XKxdX7kJI52[2yndYOgZYZ1\XJiNjDodpMh[nlibXnjdo9{[2:yaXOgZY5idHm|aYO= NE\GUYczODdyOEmzOy=>
human ReNcell VM cells NUf2do94WHKxbHnm[ZJifGmxbjDhd5NigQ>? M4j4Z|Mh|ryP NH\5WWQ4OiCq MXPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFKnTnPlcIwhXk1iY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIH;mJINmdGxicILvcIln\XKjdHnvckBifCB|IIXNJIFnfGW{IEeyJIhzew>? MmTTNlA4ODh7M{e=
human SK-MEL-1 cell MVvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NIq5XIZKdmirYnn0bY9vKG:oIHj1cYFvKFONLV3FUE0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImsJGlEPTB;M{SuO|E1KM7:TR?= NF[xVm1USU6JRWK=
human MOLT-4 cell NYe4fXg6T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2K0cWlvcGmkaYTpc44hd2ZiaIXtZY4hVU:OVD20JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|YvOTR4NzFOwG0> NVWzNFBjW0GQR1XS
human T47D cell MnToS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M{HMc2lvcGmkaYTpc44hd2ZiaIXtZY4hXDR5RDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUO3MlE3PDdizszN NX;odZZ{W0GQR1XS
human SW1710 cell MUXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NETZUlFKdmirYnn0bY9vKG:oIHj1cYFvKFOZMUexNEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVM4NjN4MEig{txO NV:wTZZnW0GQR1XS
human MKN7 cell NGDQZ2tIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NES0fYNKdmirYnn0bY9vKG:oIHj1cYFvKE2NTkegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2zPE4xQTB7IN88US=> M4rsfXNCVkeHUh?=
human CAL-72 cell MUPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3T6emlvcGmkaYTpc44hd2ZiaIXtZY4hS0GOLUeyJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|gvOTF5ODFOwG0> M2\xNnNCVkeHUh?=
human AGS cell NWPjdFZ4T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NGq0d3hKdmirYnn0bY9vKG:oIHj1cYFvKEGJUzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUO4MlIxOzlizszN NEnFeHVUSU6JRWK=
human BE-13 cell MV3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYXJcohq[mm2aX;uJI9nKGi3bXHuJGJGNTF|IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;M{iuO|c5OyEQvF2= NID4eGlUSU6JRWK=
human Calu-6 cell MYjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M{T2eWlvcGmkaYTpc44hd2ZiaIXtZY4hS2GudT22JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|kvPjl4NDFOwG0> NHu1dohUSU6JRWK=
human CAL-27 cell NV\O[VgyT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NV7tSHNxUW6qaXLpeIlwdiCxZjDoeY1idiCFQVytNlch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF01PC5yMEW0JO69VQ>? MVfTRW5ITVJ?
human BB49-HNC cell MW\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M17QNWlvcGmkaYTpc44hd2ZiaIXtZY4hSkJ2OT3IUmMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF01PC5yMke4JO69VQ>? Ml;UV2FPT0WU

... Click to View More Cell Line Experimental Data

In vivo Administration of SB 216763 at 20 mg/kg significantly prevents lung inflammation and the subsequent fibrosis in bleomycin (BLM)-induced pulmonary inflammation and fibrosis model in mice by significantly blocking the production of inflammatory cytokines MCP-1 and TNF-α by macrophages, and significantly improves the survival of BLM-treated mice. SB 216763 treatment causes a significant reduction in BLM-induced alveolitis by inhibiting alveolar epithelial cell damage. [4]

Protocol

Kinase Assay:[1]
+ Expand

GSK-3 activity assay:

GSK-3 kinase activity is measured, in the presence of various concentrations of SB 216763, in a reaction mixture containing final concentrations of 1 nM human GSK-3α, 50 mM MOPS pH 7.0, 0.2 mM EDTA, 10 mM Mg-acetate, 7.5 mM β-mercaptoethanol, 5% (w/v) glycerol, 0.01% (w/v) Tween-20, 10% (v/v) DMSO, and 28 μM GS-2 peptide substrate. The GS-2 peptide sequence corresponds to a region of glycogen synthase that is phosphorylated by GSK-3. The assay is initiated by the addition of 0.34 μCi [33P]γ-ATP. The total ATP concentration is 10 μM. Following 30 minutes incubation at room temperature the assay is stopped by the addition of one third assay volume of 2.5% (v/v) H3PO4 containing 21 mM ATP. Samples are spotted onto P30 phosphocellulose mats and washed six times in 0.5% (v/v) H3PO4. The filter mats are sealed into sample bags containing Wallac betaplate scintillation fluid. 33P incorporation into the substrate peptide is determined by counting the mats in a Wallac microbeta scintillation counter.
Cell Research:[3]
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  • Cell lines: BXPC-3, MIA-PaCa2, PANC1, ASPC1, and CFPAC cells
  • Concentrations: Dissolved in DMSO, final concentrations ~50 μM
  • Incubation Time: 24, 48, and 72 hours
  • Method: Cells are exposed to various concentrations of SB 216763 for 24, 48 and 72 hours. Relative cell viability is measured using the MTS assay. Apoptotic cells are determined by staining with Hoechst.
    (Only for Reference)
Animal Research:[4]
+ Expand
  • Animal Models: C57BL/6N mice with lung inflammation and fibrosis induced by bleomycin (BLM)
  • Formulation: Dissolved in DMSO, and diluted in saline
  • Dosages: 20 mg/kg
  • Administration: Intravenously
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 23 mg/mL (61.95 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order:
5% DMSO+corn oil
For best results, use promptly after mixing.
4mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 371.22
Formula

C19H12N2O2Cl2

CAS No. 280744-09-4
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID