CHIR-99021 (CT99021) HCl

Catalog No.S2924

CHIR-99021 (CT99021) HCl Chemical Structure

Molecular Weight(MW): 501.8

CHIR-99021 HCl (CT99021) is hydrochloride of CHIR-99021, which is a GSK-3α/β inhibitor with IC50 of 10 nM/6.7 nM; CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs Cdc2 and ERK2.

Size Price Stock Quantity  
In DMSO USD 340 In stock
USD 90 In stock
USD 170 In stock
USD 570 In stock
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Cited by 71 Publications

9 Customer Reviews

  • Differentiation induced by treatment with the Gsk3 inhibitor in hPSCs is β-catenin dependent. H9-7TGP cells were treated with 12 uM CH in mTeSR1 for 4 d. Immunofluorescent staining for Oct4, Isl1, and Nkx2.5 was compared with GFP expression. (Scale bars, 50 uM.)

    Proc Natl Acad Sci U S A 2012 109(27):E1848-57. CHIR-99021 (CT99021) HCl purchased from Selleck.

    Gpc4 regulates ESC response to Wnt/β-catenin signaling.  (E) Quantitative analysis of control and Gpc4-mutant beating foci exposed to the GSK3 inhibitor, CHIR 99021 (1 and 3 μM, from day 0 to day 5). Analysis was done at different time points. Gpc4- mutant cells treated with CHIR 99021 behave as controls (mean±SEM; n=2).

    Stem Cells 2012 30, 1863-1874. CHIR-99021 (CT99021) HCl purchased from Selleck.

  • Inhibition of GSK3 activity induces an autophagic response in human pancreatic cancer cells. PANC1 cells were treated for 24 h with DMSO, CHIR99021 (CHIR; 5 uM) or the mTOR inhibitor Torin1 (250 nM). Autophagosome (punctate LC3B) (D) and lysosome (E) labeling was visualized with a Zeiss LSM700 confocal microscope. Images were acquired with a 20x (D) or 63x (E) objective. Nuclei were stained with DAPI. Scale bars, 20 祄.

    J Biol Chem 2015 290(9), 5592-605 . CHIR-99021 (CT99021) HCl purchased from Selleck.

     

    Characterization of rES cells. A: image of normal rES cell colonies on feeder layers. B: rES colonies were positive for AP staining. CeE: rES colonies readily expressed pluripotent markers, Sox2 (C), Oct4 (D), and SSEA-1 (E). Blue, DAPI. Scale bars: 100 um.

    J Genet Genomics 2012 39, 643e651. CHIR-99021 (CT99021) HCl purchased from Selleck.

  •  

    Smad linker phosphorylation involves GSK3 in human melanoma lines. Inhibition of Smad2 and Smad3 linker phosphorylation in the presence of the specific GSK3 specific inhibitor, CT99021. 24 hours post seeding, WM793 and 1205LU cells were serum-starved for about 16 hours, and incubated in the absence (2) or presence (+) of 2 uM of CT99021 for two hours. Immunoblots were performed as in A. p: Phosphorylated. S: Serine; T: Threonine.

    PLoS One 2012 7, e47312. CHIR-99021 (CT99021) HCl purchased from Selleck.

    A. Vero cells were pretreated with the indicated concentrations of CHIR-99021 for 1 h and then infected with PEDV (MOI = 1) for 8 or 10 h. Cell lysates were prepared and analyzed using Western blotting.

    PLoS One, 2016, 11(8):e0161508. CHIR-99021 (CT99021) HCl purchased from Selleck.

  • SKOV3/DDP cells were treated with a range of concentration of (A) CHIR-99021 or in combination with 20 µM cisplatin for 48 h.

    Exp Ther Med, 2016, 12(6):3851-3858. CHIR-99021 (CT99021) HCl purchased from Selleck.

     

    PANC1 cells were treated with GSK3 inhibitors SB216763 (SB 10µM or SB 20µM) or CHIR99021 (CHIR 5µM) for 72h.

    CHIR-99021 (CT99021) HCl purchased from Selleck.

  •  

     Activity of canonical WNT/ β -CATENIN signals induced by CHIR 99021 in the developing human lung. Human lung tissue explants obtained at 15 weeks of gestation were exposed to 0, 5 and 10 μM CHIR 99021 in vitro for 72 h. (A-B) Western blot analysis showed expression of β -CATENIN in human lung tissues. Results are presented as the mean ± sem of three independent experiments. *P < 0.05, **P < 0.01. (C) qRT-PCR analysis detected a dose-dependent increase in WNT signaling transcription factors and target genes following exposure to CHIR 99021. Results are presented as the mean ± sem of three independent experiments. *P < 0.05, **P < 0.01. (D-K) Histology examination showed obvious morphogenetic changes of the airway epithelial cells associated with CHIR 99021 treated lung tubes. Scale bars: D-G =100 μm; H-K = 20 μm.

    CHIR-99021 (CT99021) HCl purchased from Selleck.

Purity & Quality Control

Choose Selective GSK-3 Inhibitors

Biological Activity

Description CHIR-99021 HCl (CT99021) is hydrochloride of CHIR-99021, which is a GSK-3α/β inhibitor with IC50 of 10 nM/6.7 nM; CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs Cdc2 and ERK2.
Targets
GSK-3β [1]
(Cell-free assay)
GSK-3α [1]
(Cell-free assay)
6.7 nM 10 nM
In vitro

CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs CDC2 and ERK2, as well as other protein kinases. Furthermore, CHIR-99021 shows only weak binding to a panel of 22 pharmacologically relevant receptors and little inhibitory activity against a panel of 23 nonkinase enzymes. CHIR-99021 induces the activation of glycogen synthase (GS) in insulin receptor-expressing CHO-IR cells with EC50 of 0.763 μM. [1] In addition to simulating the actions of insulin, inhibition of GSK-3 by CHIR-99021 (3 μM) increases free cytosolic β-catenin by 1.9-fold, mimicking the canonical Wnt signaling pathway in 3T3-L1 preadipocytes. During any of the first 3 days of differentiation, CHIR-99021 treatment inhibits the preadipocyte differentiation with IC50 of 0.3 μM by blocking induction of CCAAT/enhancer-binding protein α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ). [2] Unlike lithium chloride and AR-A014418, CHIR-99021 treatment does not reduce the viability of INS-1E cells even at high concentrations. Instead, CHIR-99021 robustly increases the rate of proliferation of INS-1E cells in a dose-dependent manner, and significantly inhibits INS-E cell death induced by high glucose and high palmitate in a concentration-dependent manner. CHIR-99021 promotes primary beta cell replication in isolated rat islets at concentrations as low as 1 μM, with 2-3 fold increase of cell replication at 5 μM of CHIR-99021 treatment. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
3T3-L1 M1\HVWZ2dmO2aX;uJGF{e2G7 NXfZXmo6OyEQvF2= NIrC[nU1KGh? M4rWZWROW09? NID2eJRKdmirYnn0d{BxemWjZHnwc4N6fGViZHnm[oVz\W62aXH0bY9v NFL6T3IyOjB3NUKwNC=>
insulin receptor–expressing CHO-IR MnPJSpVv[3Srb36gRZN{[Xl? NGTHem82ODBibl2= M{j2TVMxKG2rbh?= NV3CWWdCTE2VTx?= Mnr5RYN1cX[jdHXzJGdtgWOxZ3XuJHN6dnSqYYPlJEhIWyl? M3vjTFEzPjB4NEm3
H4IIE NEnq[IdHfW6ldHnvckBCe3OjeR?= NWfCUGZyOTBizszN NXiwXJVOOjRiaB?= NX3EfW02TE2VTx?= MnvOVoVofWyjdHXzJO6zNWOjdHXubY4h[WO2aY\peJkh[W6mIGTJVmUh[2:wdHHpcolv\yCycn;tc5RmeiCjY4Tpeol1gQ>? M3LFVFE2OzVyMUm1
Oct4/Sox2/Klf4-infected MEFs MkjpSpVv[3Srb36gRZN{[Xl? MnjTNVAh|ryP NVHUNXF2OjhiZB?= MofYSG1UVw>? M37rOXBzd22xdHXzJG1GTnNicnXwdo9oemGvbXnu[{BVemGwc3T1Z4VlKGK7IF;jeFQtKFOxeEKsJIFv\CCNbH[0 MojKNVk5OzlyNUW=
Sca-1+ HPC NXfOUYd1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVSyJO69VQ>? MYi2JIQ> Mk\SSG1UVw>? NGD6b|FUgW6ncnfpd5Rq[2GubImgdJJwdW:2ZYOgV4NiNTFtwrDIVGMheHKxbHnm[ZJifGmxbjDhcoQh[2:ub375JIZwem2jdHnvckB1d2endHjldkB4cXSqIFXHSi=> M1jDSlIyPjJ2M{S4
HMC1.2 NEPjenRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIGxVlQ{OCEQvF2= M3L0TlczKGh? MX7EUXNQ NGO1NY9T\WS3Y3XzJINmdGxic4Xyeol3[Wx? MWqyNlA{QTNyMR?=
Endothelial cell(EC) MU\GeY5kfGmxbjDBd5NigQ>? MmTuN{DPxE1? M3q0Z2ROW09? NH3SVnpRem:vb4Tld{BmdmSxdHjlcIlidCClZXzsJEhGSyliZHnm[oVz\W62aXH0bY9v Mk\XNlIxPTJyME[=
PANC1 NYnDSYJzSXCxcITvd4l{KEG|c3H5 MUNCpFUh|ryP MXO3NkBp MkfMSG1UVw>? MU\Udolo\2W{czDhckBieG:ydH;0bYMhemW|cH;ud4U> M1m2U|IzOjBzMUi2
INS-1E MWDGeY5kfGmxbjDBd5NigQ>? MXqyMlUh|ryP MWGyOEBp NFXVbXZFVVOR NWnPWVFMUW6mdXPld{BiKHKnZIXjeIlwdiCxZjDOVFEhd3[ncnX4dJJme3Orb36= MXuyNlQzPzdyNB?=
hiPSC-NPs NHmzTllIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mkf5N-KBkc7:TR?= MUe3NkBp M2DKTWROW09? MY\JcoNz\WG|ZYOgZ4VtdCCycn;sbYZmemG2aX;uxsA> NXrDWHBDOjJ4NEK2PFc>
platelets MmLjSpVv[3Srb36gRZN{[Xl? NX\KSZZCOTBizszN NHKzfZYyPSCvaX6= MXzEUXNQ NF;hPYpKdmO{ZXHz[ZMhfGi{b33ibY4udWWmaXH0[YQheGyjdHXs[ZQh[WepcnXnZZRqd25iYX7kJGFVWCC|ZXPy[ZRqd25? M{js[|I{OjN7OEe3
DN3 MknFSpVv[3Srb36gRZN{[Xl? MoXzNVAh|ryP NU\sOplSQTZiaB?= NWPLNWtyTE2VTx?= M2HlUGlv[3KnYYPld{Bk\WyuIHTleoVtd3CvZX70JIFv\CCycn;sbYZmemG2aX;u NF23UWYzOzV{Nkm4PS=>
embryonic stem cells (ESC) NYfSNpY6TnWwY4Tpc44hSXO|YYm= NVLIXZI5OyEEtV2= M{nFN|EyKGR? NYrETGhNTE2VTx?= NESw[XlRem:vb4Tld{B{\WyoLYLlcoV4[Wxib3[gcoHEt3[nLYT5dIUhdW:3c3Wg[Y1jenmxbnnjJJN1\W1iY3XscJMhMEWVQzm= MUCyN|g1OzNzMh?=
H460 MmS2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NInXN4I2KML3TR?= M33VNVI1KGh? MlLMSG1UVw>? NFS4OIRRem:vb4Tld{Bk\WyuIIDyc4xq\mW{YYTpc44> MWSyOFM3PzZ6OB?=
H157 NIL4OWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUe1JOK2VQ>? M1XnfFI1KGh? MoPDSG1UVw>? NH;pNZFRem:vb4Tld{Bk\WyuIIDyc4xq\mW{YYTpc44> NFXpZ|EzPDN4N{[4PC=>
H460 NInSS5RCeG:ydH;zbZMhSXO|YYm= NXnXdI9ZPSEEtV2= NIXOVYMzPCCq MXjEUXNQ NXXUSVN5SXS2ZX71ZZRmeyClZXzsJIFxd3C2b4Ppdy=> NVfjRoRoOjR|Nke2PFg>
H157 Mk\vRZBweHSxc3nzJGF{e2G7 M4\zZlUhyrWP M1K4d|I1KGh? NE\uWHFFVVOR NEfpXFZFd2W|IH7veEB{cWewaX\pZ4FvfGy7IHHm[oVkfCCjcH;weI9{cXN? NFmwSo4zPDN4N{[4PC=>
A549 MU\BdI9xfG:|aYOgRZN{[Xl? MnvZOUDDvU1? NYPLco1ZOjRiaB?= MXTEUXNQ M2LtTGlv[3KnYYPld{Bk\WyuIHHwc5B1d3Orcx?= MYCyOFM3PzZ6OB?=
Neural progenitor cells NWfRXY9PTnWwY4Tpc44hSXO|YYm= M4HtTVMh|ryP NYjhbIRsOTBiZB?= MVnEUXNQ MoLsTY5lfWOnczDn[Y5memG2aX;uJI9nKG6ndYLhcEBxem:pZX7peI9zKGOnbHzz MYSyOFY{QDB|NB?=
ES-D3 NHrXWIJEgXSxdH;4bYMhSXO|YYm= NYG1co5yOTEEoN88US=> Ml\1N{Bl M2TJNmROW09? M2rWeGlv\HWlZYOgcY9l\XKjdHWgZ5l1d3SxeHnjbZR6KHerdHigTWM2OCCxZjC0Mlkh|ryP MUmyOFc4QTN4NR?=
ES-CCE MYfDfZRwfG:6aXOgRZN{[Xl? M1PyclExyqEQvF2= MY[zJIQ> NXy0[5BrTE2VTx?= MWrJcoR2[2W|IH3v[IVz[XSnIHP5eI91d3irY3n0fS=> NIPRd3AzPDd5OUO2OS=>
ES-D3 NV;1bm5lTnWwY4Tpc44hSXO|YYm= NV;rSnJEPcLizszN MkO4N{Bl M3i0e2ROW09? M3naPHN1em:wZ3z5JIlv\HWlZYOgeIhmKGGldHn2bZR6KG:oIGfueE9j\XSjLXPheIVvcW5icHH0bJdigQ>? NEjZb|IzPDd5OUO2OS=>
ES-CCE MXnGeY5kfGmxbjDBd5NigQ>? MUm1xsDPxE1? NXzvVmx[OyCm M4Dqe2ROW09? Ml\5V5Rzd26pbImgbY5lfWOnczD0bIUh[WO2aY\peJkhd2ZiV370M4JmfGFvY3H0[Y5qdiCyYYToe4F6 M1ThU|I1Pzd7M{[1
SW982 NGe4[JJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUDOcGprUUN3ME2wMlIyPDF4IN88US=> MVTTRW5ITVJ?
GCT MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkHsTWM2OD1yLkOwOlczKM7:TR?= MnTzV2FPT0WU
769-P MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4K5emlEPTB;MD61PFQ6QSEQvF2= MYLTRW5ITVJ?
LOXIMVI M3TtZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1nUUGlEPTB;MD61PFg{OiEQvF2= NGXoc5NUSU6JRWK=
D-336MG MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYPJR|UxRTBwOES2NkDPxE1? MnyyV2FPT0WU
S-117 MneyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MW\JR|UxRTBwOUKxN|Eh|ryP NV3mVmk1W0GQR1XS
D-247MG NViwZox[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn;JTWM2OD1yLkm0PFUzKM7:TR?= MlXsV2FPT0WU
TYK-nu MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX\DOGtlUUN3ME2xMlA4QTF{IN88US=> M4S2OnNCVkeHUh?=
A498 NUfWRZRlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3r5fWlEPTB;MT6zNFQ3QSEQvF2= M3ewSXNCVkeHUh?=
BCPAP MmH6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVjJR|UxRTFwNk[4PVgh|ryP MYXTRW5ITVJ?
KYSE-180 NFTQbXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFXWflNKSzVyPUGuO|I4QDhizszN NUnzR|doW0GQR1XS
MIA-PaCa-2 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NE\kcppKSzVyPUGuO|U6PzdizszN MlHRV2FPT0WU
HCC1395 MoXkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MonyTWM2OD1zLke4Nlc{KM7:TR?= M{W1XXNCVkeHUh?=
RS4-11 MnHRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1\oSmlEPTB;MT65OVQyPSEQvF2= NI\KNGxUSU6JRWK=
NCI-H2122 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYrJR|UxRTJwMke2PFQh|ryP M1OzR3NCVkeHUh?=
SNU-423 MmHGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnTOTWM2OD1{LkS5O|g4KM7:TR?= M4Phb3NCVkeHUh?=
SK-LMS-1 M4f0eWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHT0WoFKSzVyPUKuOlQyOjhizszN MX;TRW5ITVJ?
RPMI-7951 NWPJV2xDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYrJR|UxRTJwN{CxPVMh|ryP MnLiV2FPT0WU
COLO-829 NHfUbohIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4PENGlEPTB;Mj63OlU1KM7:TR?= MYnTRW5ITVJ?
SCC-9 NGPzXWdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVTkXXNUUUN3ME2yMlk2PDh3IN88US=> NEW4dVVUSU6JRWK=
A704 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mle4TWM2OD1|LkC4NVIh|ryP NYPoOWszW0GQR1XS
HOP-62 NFTsSWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2DwXWlEPTB;Mz6zOlA2PCEQvF2= NHSxb2xUSU6JRWK=
HCC1569 NYH5fGRQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXfJR|UxRTNwNEizNFUh|ryP NVzSSYRIW0GQR1XS
A172 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MW\JR|UxRTNwN{W0PVgh|ryP NIfWS5lUSU6JRWK=
MOLT-16 NXfM[HJbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn;KTWM2OD1|Lki4OVU3KM7:TR?= NWDxTFg3W0GQR1XS
TE-15 M3PGfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHXvdmFKSzVyPUOuPVU{PDNizszN M{n4THNCVkeHUh?=
OE19 MmnRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV\zVpI4UUN3ME2zMlk3PDVzIN88US=> M{\2XnNCVkeHUh?=
MHH-ES-1 NW\YXoE4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIO5fpNKSzVyPUSuNFQ2PDVizszN M{DiNXNCVkeHUh?=
NKM-1 M4Cz[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYXJR|UxRTRwMUO5O|Eh|ryP M3jlUHNCVkeHUh?=
RCC10RGB M4DnPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2q3c2lEPTB;ND6yPVQ4QSEQvF2= MX\TRW5ITVJ?
BxPC-3 M3frPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWHJR|UxRTRwN{CzPVEh|ryP NGfSfoxUSU6JRWK=
ALL-PO MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGTtdWZKSzVyPUSuPFM5PThizszN M2DCWnNCVkeHUh?=
SK-OV-3 NHvvWG1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlPVTWM2OD12Lkm5N|Qh|ryP NHu2TXlUSU6JRWK=
SW1710 MlGzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3:xTWlEPTB;NT60NlQ2PiEQvF2= NH\sRZNUSU6JRWK=
NCI-H2452 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXHncZlOUUN3ME21MlQ2PjJ6IN88US=> Ml:3V2FPT0WU
D-392MG NIXZSVhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{TmeGlEPTB;NT60PVE1PSEQvF2= NVLVZVJzW0GQR1XS
LB2518-MEL M2Lacmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGjtXVhKSzVyPUWuO|I3OjdizszN M3nDWHNCVkeHUh?=
BL-41 Mm\kS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlrtTWM2OD13LkixNlY3KM7:TR?= Ml;sV2FPT0WU
WM-115 NWTVZ3JZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlzFTWM2OD13LkixOVA6KM7:TR?= Mnm0V2FPT0WU
SK-MEL-2 NHnpc5RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWPUSGFHUUN3ME22MlM5OzR2IN88US=> Mon1V2FPT0WU
CGTH-W-1 NHHtSo5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYXVfJdoUUN3ME22MlQyPyEQvF2= NF6yepJUSU6JRWK=
BB65-RCC MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH6yRXNKSzVyPU[uOVMyQTVizszN MVvTRW5ITVJ?
CAL-85-1 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIjsZndKSzVyPU[uOlA6OzZizszN NUjwWmpnW0GQR1XS
RT-112 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkPnTWM2OD15LkG2OFU3KM7:TR?= M3;PO3NCVkeHUh?=
MC116 NV3NZXpuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVjJR|UxRTdwMki2PVEh|ryP MmW1V2FPT0WU
G-361 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MU\JR|UxRTdwNE[1OlEh|ryP Mn\YV2FPT0WU
SW1417 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV3JR|UxRTdwNkS1PFQh|ryP NWLxXlM1W0GQR1XS
MV-4-11 MlfhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFXt[GNKSzVyPUiuOVQ{ODRizszN MV7TRW5ITVJ?
CAL-72 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXvJR|UxRThwN{[xNVMh|ryP MYLTRW5ITVJ?
MKN28 Mm\BS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NESx[I1KSzVyPUiuPFg2OTZizszN NFe5ZWRUSU6JRWK=
MEG-01 NWjsem5UT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4raSWlEPTB;OD65PVA1OyEQvF2= NGrWd49USU6JRWK=
ACHN MkLoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NG\vVXVKSzVyPUmuN|A2PDZizszN MkTvV2FPT0WU
NCI-H1755 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX7JR|UxRTlwM{m5PFYh|ryP M{jENnNCVkeHUh?=
NCI-H64 M2DicGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mm\ITWM2OD17LkSyNlU2KM7:TR?= M4P2[XNCVkeHUh?=
COLO-205 M1nVeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHexV4JKSzVyPUmuOFM5OjFizszN MmnXV2FPT0WU
CTV-1 M{jUSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{\BeWlEPTB;OT60N|g6PCEQvF2= NHT6WHlUSU6JRWK=
U-2-OS MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MniyTWM2OD17LkS1NFA3KM7:TR?= MmjHV2FPT0WU
LB996-RCC NGLXdHZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnzqTWM2OD17LkWyN|k4KM7:TR?= NYDyXoJzW0GQR1XS
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EFM-19 NIDUZ5JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYjJR|UxRTlwN{W1OlYh|ryP NELHcGRUSU6JRWK=
DBTRG-05MG M1\wNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXjNdWVMUUN3ME25Mlc4PzdizszN M{\SOXNCVkeHUh?=
U-118-MG NFP3fFBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWDzOZpFUUN3ME25Mlk1PDR2IN88US=> NEDDNXNUSU6JRWK=
LB1047-RCC M{TKWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MU\JR|UxRTlwOUm3PVgh|ryP NXLqPGplW0GQR1XS
NCI-H28 NF3mfXBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MU\JR|UxRTFyLkS0NVMh|ryP M1OxNnNCVkeHUh?=
MZ2-MEL MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3;UU2lEPTB;MUCuOFY4PyEQvF2= NVfCTZdnW0GQR1XS
M059J M1S0c2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3GxNGlEPTB;MUCuOlExOiEQvF2= NXfaTG1oW0GQR1XS
HT-3 M4jGZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUPJR|UxRTFyLk[xO|kh|ryP M3z1S3NCVkeHUh?=
SNU-387 NYfNW|I3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M33qe2lEPTB;MUCuO|I{PSEQvF2= M1zsSHNCVkeHUh?=
NCI-H526 NWn0Wo14T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV3JR|UxRTFzLkGzOlUh|ryP MX;TRW5ITVJ?
HEL MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MY\JR|UxRTFzLk[1O|kh|ryP M{TFb3NCVkeHUh?=
PA-1 M4HVemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUPCV3dGUUN3ME2xNU46OyEQvF2= NWfGenpZW0GQR1XS
PC-14 NHfrXplIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEnGU2VKSzVyPUGyMlMzPCEQvF2= MUnTRW5ITVJ?
C8166 M1TF[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1LNZmlEPTB;MUOuNlMyQCEQvF2= NX7McodFW0GQR1XS
NCI-H82 NUfuV|FWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NI\aSZZKSzVyPUGzMlI6ODlizszN MlT3V2FPT0WU
DSH1 M{jyS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn;0TWM2OD1zMz6zPFc6KM7:TR?= NE[w[IxUSU6JRWK=
T98G MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGnNRWFKSzVyPUGzMlQ4OTdizszN MWTTRW5ITVJ?
KU812 M2m2dmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWLJR|UxRTF|LkWxO|Uh|ryP NHzTOYdUSU6JRWK=
SW48 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYXJR|UxRTF|LkeyOlUh|ryP MVjTRW5ITVJ?
MEL-HO MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGH1cGpKSzVyPUGzMlg5OjlizszN MYjTRW5ITVJ?
SF539 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlLuTWM2OD1zND6wNVE3KM7:TR?= M4nJTHNCVkeHUh?=
NB1 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnHmTWM2OD1zND6xPVM2KM7:TR?= MoKwV2FPT0WU
ST486 NXHuT2FzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWKxT3pmUUN3ME2xOE4zOjVzIN88US=> M{XaV3NCVkeHUh?=
LoVo NFKxWnFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2T2dWlEPTB;MUWuNlY2QSEQvF2= M2fCSXNCVkeHUh?=
NH-12 MoqwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3H1cGlEPTB;MU[uNFA6PyEQvF2= NFPUSFBUSU6JRWK=
CCRF-CEM NYPDSJJVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{izTmlEPTB;MU[uOlcxOiEQvF2= Mn3SV2FPT0WU
Daudi NX3WbndVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NU\4VYt[UUN3ME2xO{43OTR7IN88US=> NWXwZXpUW0GQR1XS
SIMA NXTjUYZlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXXRVWZoUUN3ME2xO{46OTF6IN88US=> M4PyOHNCVkeHUh?=
8305C M4nNfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYPJR|UxRTF6LkWzNFUh|ryP M4jCXHNCVkeHUh?=
EM-2 M2e5Z2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGO5So5KSzVyPUG4MlYzPDhizszN MWjTRW5ITVJ?
SNU-449 M4\HNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGTlOoJKSzVyPUG4MlcyPSEQvF2= M3HxVXNCVkeHUh?=
A101D NH7rUFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWPhUlZHUUN3ME2xPU4zPDNizszN NEK2e|JUSU6JRWK=
MHH-NB-11 MoTtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIWxSm1KSzVyPUG5MlU{PDJizszN MYHTRW5ITVJ?
Becker MkLzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{mwUmlEPTB;MkCuNVc4PyEQvF2= NXrHcFlxW0GQR1XS
NCI-H727 MnrYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXLJR|UxRTJyLkKxOlkh|ryP Mmq3V2FPT0WU
HCC1187 NGTRfZZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NITLdmhKSzVyPUKwMlM2QDlizszN M{XONXNCVkeHUh?=
KYSE-450 NEfjUlBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MknMTWM2OD1{MT61N|U1KM7:TR?= M{\OV3NCVkeHUh?=
SK-MEL-28 NXztWWl1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUfJR|UxRTJzLk[4OlMh|ryP MmXJV2FPT0WU
BPH-1 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NETh[YZKSzVyPUKxMlcxOzVizszN M4XlUnNCVkeHUh?=
EoL-1- M{WwNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVrJR|UxRTJzLkizPFUh|ryP M3HxWnNCVkeHUh?=
TGBC24TKB MofYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1GxWmlEPTB;MkGuPFk2PyEQvF2= M4Plb3NCVkeHUh?=
KARPAS-299 NUjkNZltT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWr0eHV7UUN3ME2yNk4yQTV2IN88US=> NVm5dI9NW0GQR1XS
SW684 MlX6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mnv3TWM2OD1{Mj6yPFU5KM7:TR?= NFXtS3VUSU6JRWK=
RXF393 NUPhcpNXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEPoe4ZKSzVyPUKyMlYxPjhizszN M1XPeHNCVkeHUh?=
LS-513 M2nwZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoW1TWM2OD1{Mj62NVkzKM7:TR?= MofoV2FPT0WU
KURAMOCHI NGi3XXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoD3TWM2OD1{Mj63NFY6KM7:TR?= MX\TRW5ITVJ?
GI-1 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4X1OWlEPTB;MkKuO|g1OSEQvF2= Mnr1V2FPT0WU
M14 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NELLTndKSzVyPUKzMlEyOzJizszN Mn;lV2FPT0WU
REH M1HVW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWTlfIlRUUN3ME2yN{4yPjl7IN88US=> M{XIUHNCVkeHUh?=
K5 M2q3d2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M13lOmlEPTB;MkOuOVQ5PiEQvF2= MUDTRW5ITVJ?
PANC-10-05 Mn2yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYnRXod{UUN3ME2yOE4xQDl6IN88US=> NHXvbnNUSU6JRWK=
HMV-II NXvKPVhJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYrJR|UxRTJ2LkKxOlMh|ryP NITUe3VUSU6JRWK=
NCI-H1963 M{nTSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2jkU2lEPTB;MkSuNlg{QSEQvF2= NXTqUFJFW0GQR1XS
LU-139 NUPpR5JyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3LtbGlEPTB;MkSuPFM2KM7:TR?= NYrTR4NuW0GQR1XS
KASUMI-1 NWXlW5VJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnH0TWM2OD1{ND65N|I5KM7:TR?= M4TlOnNCVkeHUh?=
RO82-W-1 MlzRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWDJR|UxRTJ4LkezO|ch|ryP M3XjUXNCVkeHUh?=
NCI-H1770 Mo\RS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYCwTVVLUUN3ME2yOk45QTh4IN88US=> NHXkV2JUSU6JRWK=
SNG-M NHm3OpRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4SxOGlEPTB;MkeuNlI4OSEQvF2= NEmxT3lUSU6JRWK=
KLE NWnjXIhbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmnGTWM2OD1{Nz6yOVY2KM7:TR?= MnXkV2FPT0WU
NCI-H2170 M4nWdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmniTWM2OD1{Nz61NlgzKM7:TR?= MYDTRW5ITVJ?
COLO-792 Mn[yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV3BU|JjUUN3ME2yO{44PzJ6IN88US=> MXjTRW5ITVJ?
ES8 MnzXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIHibGJKSzVyPUK3Mlc5OTRizszN NIHiSlZUSU6JRWK=
MC-CAR MkniS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYjJR|UxRTJ5Lke5NFch|ryP NX\2RZVDW0GQR1XS
SU-DHL-1 M3W5Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlO1TWM2OD1{Nz65OFc1KM7:TR?= MW\TRW5ITVJ?
OAW-28 NIT3NlhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGDBRlRKSzVyPUK4MlAxPTJizszN NGjvfGRUSU6JRWK=
C32 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnTuTWM2OD1{OD6wPFI3KM7:TR?= M1fQfHNCVkeHUh?=
SW872 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUHDVY41UUN3ME2yPE42OzJ4IN88US=> MonGV2FPT0WU
JiyoyeP-2003 NHP5[3RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlrITWM2OD1{OT62O|E6KM7:TR?= NIjiVlVUSU6JRWK=
A204 MlTlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2fKbmlEPTB;MkmuOlgzQCEQvF2= MUjTRW5ITVJ?
IST-MES1 MoezS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mm[zTWM2OD1{OT63NFkzKM7:TR?= M1uxT3NCVkeHUh?=
LCLC-103H M{f1dWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIP0VYlKSzVyPUOwMlIxPTlizszN MmTDV2FPT0WU
CAKI-1 NFrvO2hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGXSU41KSzVyPUOwMlI3PTZizszN NIPWVHZUSU6JRWK=
NALM-6 NVHaNVJKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUHJR|UxRTNyLkS5OlUh|ryP MXzTRW5ITVJ?
SK-MM-2 MoTnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{frSmlEPTB;M{CuPVc6OiEQvF2= MmDmV2FPT0WU
LB373-MEL-D Mn7GS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV[3UFJHUUN3ME2zNU4xODR5IN88US=> NFzRcndUSU6JRWK=
CAS-1 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NILscZhKSzVyPUOxMlc3PjVizszN MXHTRW5ITVJ?
KNS-42 M3m5b2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH7jVlJKSzVyPUOxMlg3PjJizszN NIXOUGtUSU6JRWK=
SW948 NEfCbVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF;PZ3lKSzVyPUOyMlc2PDhizszN NV22TmtZW0GQR1XS
HCC1143 NHnET4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIHuVmhKSzVyPUOyMlc6PzlizszN NXnpT2VSW0GQR1XS
NCI-H1648 Mm\QS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX;JR|UxRTN{Lki0Olch|ryP NWHne3FCW0GQR1XS
RVH-421 M1\2OWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXHJR|UxRTN|LkG2PEDPxE1? M1z6ZnNCVkeHUh?=
KU-19-19 NIC2c4NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml7nTWM2OD1|Mz63OVM1KM7:TR?= NE[xSZJUSU6JRWK=
PFSK-1 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NE\IbodKSzVyPUO0MlkzPDFizszN NFXafYZUSU6JRWK=
TK10 Ml3lS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWDJR|UxRTN3LkGzNlIh|ryP M37mdXNCVkeHUh?=
IGROV-1 NIrUeG1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkP5TWM2OD1|NT6zOFE3KM7:TR?= M2nDSXNCVkeHUh?=
EFE-184 NF\Mdm1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWrJR|UxRTN3LkmxO{DPxE1? MlruV2FPT0WU
8505C NYHnU4E{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4HjZmlEPTB;M{WuPVI6PCEQvF2= MXjTRW5ITVJ?
SK-UT-1 NET2PZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3nEdWlEPTB;M{[uN|M{OSEQvF2= NW\yUWtVW0GQR1XS
MKN1 MlnnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlLnTWM2OD1|Nj64OFQ1KM7:TR?= MkLZV2FPT0WU
no-10 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVH2R5ZpUUN3ME2zPE4yOjl2IN88US=> MoLiV2FPT0WU
HT-144 NVPVRnZTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHjYSFZKSzVyPUO4MlI2PzVizszN MVjTRW5ITVJ?
CMK NIfibYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUPOPZF5UUN3ME2zPE41OTZ{IN88US=> MVHTRW5ITVJ?
KM12 M2DmUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXXJR|UxRTN6LkW5PVgh|ryP MUPTRW5ITVJ?
HCC2218 NH2zdmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1j5dGlEPTB;NECuOVg1QSEQvF2= NHnqXWxUSU6JRWK=
BE-13 Mm[1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYPJR|UxRTRzLkS0O|Mh|ryP M4\UXHNCVkeHUh?=
SK-CO-1 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV7JR|UxRTRzLke3OVUh|ryP NYTDT4dVW0GQR1XS
MPP-89 M1nVRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2rVZWlEPTB;NEGuPVIyOSEQvF2= M3fzVnNCVkeHUh?=
NY NHHXZmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoLrTWM2OD12Mj6xPFAyKM7:TR?= NVnh[WlKW0GQR1XS
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HCT-116 M2PpSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHvsdpVKSzVyPUSzMlIxQTlizszN M3S3NnNCVkeHUh?=
LB831-BLC NYLlNpRiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1H5XGlEPTB;NEOuOlk4KM7:TR?= M{TnSnNCVkeHUh?=
TE-8 NHGwRohIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2W3b2lEPTB;NEOuO|g6PSEQvF2= NV3lSXp3W0GQR1XS
SR MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVHJR|UxRTR|Lki2O|Mh|ryP NXjPR2FZW0GQR1XS
ESS-1 M2iwcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXTDNI9lUUN3ME20OE4zODZ{IN88US=> MlOyV2FPT0WU
KYSE-270 Moi3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWnKbVVJUUN3ME20OU4xPDN5IN88US=> NXLOSnp5W0GQR1XS
H4 Mn;IS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml\kTWM2OD12NT6zNlk3KM7:TR?= M1z2UnNCVkeHUh?=
COLO-800 NVKxeHhVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFTHR4tKSzVyPUS1MlU5QThizszN MW\TRW5ITVJ?
Ramos-2G6-4C10 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnLDTWM2OD12Nj6wO|U2KM7:TR?= M1[0OHNCVkeHUh?=
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UACC-62 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVzJR|UxRTR4Lki2NFgh|ryP NWXrfI9zW0GQR1XS
D-542MG NH3FTXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3\jXWlEPTB;NEeuOFU5QSEQvF2= MmT3V2FPT0WU
NCI-H747 MmjCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWexZW57UUN3ME20O{44OjZ2IN88US=> NGjPdWRUSU6JRWK=
HCC2998 NHfOOpJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVLUPXBqUUN3ME20PE4zQTh{IN88US=> NF\pNFFUSU6JRWK=
SNB75 M1HUTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWPJR|UxRTR6LkSxNVgh|ryP MX3TRW5ITVJ?
COR-L105 NXPYcXkxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1;3OmlEPTB;NEiuPVc{KM7:TR?= M3fOVHNCVkeHUh?=
SNU-C1 NELKcG9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1\SSmlEPTB;NEmuN|IxPyEQvF2= MmL3V2FPT0WU
LC4-1 NIPGbJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXLJR|UxRTR7Lki5NVIh|ryP NVHuW2I4W0GQR1XS
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LS-123 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4nCdGlEPTB;M{K4MlE3PCEQvF2= NHXISodUSU6JRWK=
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MN-60 NH3mOo1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXTnNHluUUN3ME2zPVMvQTR6IN88US=> MlXPV2FPT0WU
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NCI-H2141 MknBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFPLSW1KSzVyPUSyOE4{QDhizszN MXrTRW5ITVJ?
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EC-GI-10 NHnrcZBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2\ZVWlEPTB;NUCyMlE3QSEQvF2= MV;TRW5ITVJ?
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NCI-H2171 NVXL[lZoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M13hVGlEPTB;NUGyMlcxPSEQvF2= MlTtV2FPT0WU
Mo-T NXLSeYtNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEe2d2NKSzVyPUWxOU4yOTZizszN MkDmV2FPT0WU
HL-60 NVLCeppMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4jnWmlEPTB;NUKyMlkzPCEQvF2= M{\uXHNCVkeHUh?=
NCI-H1436 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF7MVmlKSzVyPUWyN{4xPjRizszN NInKXnlUSU6JRWK=
NCI-H510A Mn3WS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mm\rTWM2OD13MkSuOlk3KM7:TR?= MVLTRW5ITVJ?
NCI-H2081 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV\JR|UxRTV{NT61NVMh|ryP NXXTdFRIW0GQR1XS
NB5 MmjWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2XD[GlEPTB;NUK2MlQ{KM7:TR?= MUHTRW5ITVJ?
NCI-H2227 NHvqZZVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX;JR|UxRTV|MD6yOVQh|ryP MVPTRW5ITVJ?
HSC-4 NEHBblhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVPTcm9GUUN3ME21N|kvOjl|IN88US=> M2jZeXNCVkeHUh?=
RL M1fuWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoP4TWM2OD13NEKuOFQzKM7:TR?= NYSyUmg3W0GQR1XS
EVSA-T NGPKWpVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlvUTWM2OD13NEOuOlg1KM7:TR?= MVzTRW5ITVJ?
NCI-H889 M4HNfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXzJR|UxRTV2Nz6yN{DPxE1? NWPGXXdqW0GQR1XS
COLO-320-HSR NF\2[49Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYXJR|UxRTV3MT6zOUDPxE1? NXnqVVBqW0GQR1XS
EW-11 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYTJR|UxRTV3Mj6zPVkh|ryP MV3TRW5ITVJ?
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CAL-148 M1r4Umdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVjJUmpbUUN3ME21OlUvODh7IN88US=> MoHxV2FPT0WU
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HT MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXHlV5VrUUN3ME22NlIvPzh3IN88US=> MmLxV2FPT0WU
NCI-H226 NEjISW9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUPJR|UxRTZ{OD6zOVMh|ryP NI\JVo5USU6JRWK=
L-363 NV7q[YN5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1j5PGlEPTB;NkS4MlA3PCEQvF2= M2S2OHNCVkeHUh?=
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TUR MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn20TWM2OD14N{GuN|IzKM7:TR?= MVLTRW5ITVJ?
NCI-SNU-16 NHPVRXJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NE\aXWpKSzVyPU[4NE41PjJizszN NYDzN5ZuW0GQR1XS
NCI-SNU-1 M1TtUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4D5VGlEPTB;NkmwMlE4OiEQvF2= NFe2Z|NUSU6JRWK=
U-87-MG MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mmm0TWM2OD14OUSuOVg2KM7:TR?= M37pd3NCVkeHUh?=
KM-H2 MnLWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{\pbmlEPTB;Nkm5MlA2QSEQvF2= NYWwUoxyW0GQR1XS
DEL NIT2OYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFHZNHlKSzVyPUewNE45OzhizszN NITRcHNUSU6JRWK=
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BC-3 MnO1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUXJR|UxRThyNT6yNFMh|ryP MXHTRW5ITVJ?
NCI-H1155 NUjiU3NnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1nRZmlEPTB;OEG4MlA2QSEQvF2= NEHad|BUSU6JRWK=
NCI-H1581 M3v5Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmDqTWM2OD17MECuNlMyKM7:TR?= NUDXUYxMW0GQR1XS

... Click to View More Cell Line Experimental Data

In vivo Oral administration of CHIR-99021 at 30 mg/kg enhances glucose metabolism in a rodent model of type 2 diabetes, with a maximal plasma glucose reduction of nearly 150 mg/dl 3-4 hours after administration, while plasma insulin remains at or below control levels. Oral administration of CHIR-99021 at 16 or 48 mg/kg 1 hour before oral glucose challenges in ZDF rats significantly improves glucose tolerance with 14% and 33% reduction in plasma glucose at 16 mg/kg and 48 mg/kg, respectively, and the higher dose of CHIR-99021 also reduces hyperglycemia before the oral glucose challenge. [1] Administration of CHIR-99021 significantly augments hematopoietic repopulation in recipient mice transplanted with mouse or human hematopoietic stem cells (HSCs), suggesting that GSK-3 is a specific modulator of HSC activity. [4]

Protocol

Kinase Assay:

[1]

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Cell-free kinase assays:

Polypropylene 96-well plates are filled with 300 μL/well buffer (50 mM tris HCl, 10 mM MgCl2, 1 mM EGTA, 1 mM dithiothreitol, 25 mM β-glycerophosphate, 1 mM NaF, 0.01% BSA, pH 7.5) containing 27 nM GSK-3α or 29 nM GSK-3β, and 0.5 μM biotin-CREB peptide substrate. Various concentrations of CHIR-99021 are added in 3.5 μL of DMSO, followed by 50 μL of ATP stock to yield a final concentration of 1 μM ATP in all cell-free assays. After incubation, triplicate 100-μL aliquots are transferred to Combiplate eight plates containing 100 μL/well 50 μM ATP and 20 mM EDTA. After 1 hour, the wells are rinsed five times with PBS, filled with 200 μL of scintillation fluid, sealed, left 30 minutes, and counted in a scintillation counter. All steps are performed at room temperature.
Cell Research:

[3]

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  • Cell lines: INS-1E
  • Concentrations: Dissolved in DMSO, final concentrations ~ 20 μM
  • Incubation Time: 1, or 4 days
  • Method:

    Cells are maintained for 24 hours in starvation medium (culture medium with only 5 mM glucose, 1% fetal calf serum). Then cells are exposed to various concentrations of CHIR-99021 for 1, or 4 days. Cell number is measured by staining of cellular DNA with CyQuant dye, which becomes fluorescent when bound to DNA. Fluorescence is measured after 30 minutes of incubation using the FLUOstar Optima reader. Cell replication is determined by BrdUrd incorporation. BrdUrd labeling solution is added to the medium for the last 4 hours before the cells are fixed using FixDenat solution and incubated with monoclonal anti-BrdUrd-POD antibodies. After substrate solution is added to each well, the light emission is measured in a microplate luminometer using the Analyst HT detection system.


    (Only for Reference)
Animal Research:

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  • Animal Models: Female db/db mice or male ZDF rats with type 2 diabetes
  • Formulation: Formulated as solutions in 20 mM citrate-buffered 15% Captisol or as fine suspensions in 0.5% carboxymethylcellulose.
  • Dosages: ~48 mg/kg
  • Administration: Orally
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 93 mg/mL (185.33 mM)
Ethanol 2 mg/mL (3.98 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order:
5% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
24mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 501.8
Formula

C22H18Cl2N8.HCl

CAS No. 1797989-42-4
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    I recently purchased CHIR 99021 to inject mice intraperitoneally. The data sheet says using 10% captisol for in vivo use. Is it for IP injection? Or do you have other solutions to suggest to use for in vivo IP injection of CHIR 99021?

  • Answer:

    The formulation of 15% Captisol is for oral gavage, and S2924 CHIR-99021 (CT99021) HCl in it is a suspension. S2924 CHIR-99021 (CT99021) HCl can be dissolved in 5% DMSO+30% PEG 300+ddH2O at 24mg/ml as a clear solution for IP injection.

  • Question 2:

    How about the half-life of this compound? If it is necessary for daily treatment when the compound is used in long-term experiment?

  • Answer:

    Please change the culture medium each day to add the new comound in it if the duration is more than 72h.

  • Question 3:

    What is the difference between S2924 and S1263? Which one is better for differentiation?

  • Answer:

    S1263 is the free base format of Chir-99021, while S2924 is the HCl salt format. They have the same biological activity but are slightly different in solubility.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID