CHIR-99021 (CT99021) HCl

Catalog No.S2924

CHIR-99021 (CT99021) HCl Chemical Structure

Molecular Weight(MW): 501.8

CHIR-99021 HCl (CT99021) is hydrochloride of CHIR-99021, which is a GSK-3α/β inhibitor with IC50 of 10 nM/6.7 nM; CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs Cdc2 and ERK2.

Size Price Stock Quantity  
In DMSO USD 340 In stock
USD 90 In stock
USD 170 In stock
USD 570 In stock
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Cited by 70 Publications

9 Customer Reviews

  • Differentiation induced by treatment with the Gsk3 inhibitor in hPSCs is β-catenin dependent. H9-7TGP cells were treated with 12 uM CH in mTeSR1 for 4 d. Immunofluorescent staining for Oct4, Isl1, and Nkx2.5 was compared with GFP expression. (Scale bars, 50 uM.)

    Proc Natl Acad Sci U S A 2012 109(27):E1848-57. CHIR-99021 (CT99021) HCl purchased from Selleck.

    Gpc4 regulates ESC response to Wnt/β-catenin signaling.  (E) Quantitative analysis of control and Gpc4-mutant beating foci exposed to the GSK3 inhibitor, CHIR 99021 (1 and 3 μM, from day 0 to day 5). Analysis was done at different time points. Gpc4- mutant cells treated with CHIR 99021 behave as controls (mean±SEM; n=2).

    Stem Cells 2012 30, 1863-1874. CHIR-99021 (CT99021) HCl purchased from Selleck.

  • Inhibition of GSK3 activity induces an autophagic response in human pancreatic cancer cells. PANC1 cells were treated for 24 h with DMSO, CHIR99021 (CHIR; 5 uM) or the mTOR inhibitor Torin1 (250 nM). Autophagosome (punctate LC3B) (D) and lysosome (E) labeling was visualized with a Zeiss LSM700 confocal microscope. Images were acquired with a 20x (D) or 63x (E) objective. Nuclei were stained with DAPI. Scale bars, 20 祄.

    J Biol Chem 2015 290(9), 5592-605 . CHIR-99021 (CT99021) HCl purchased from Selleck.

     

    Characterization of rES cells. A: image of normal rES cell colonies on feeder layers. B: rES colonies were positive for AP staining. CeE: rES colonies readily expressed pluripotent markers, Sox2 (C), Oct4 (D), and SSEA-1 (E). Blue, DAPI. Scale bars: 100 um.

    J Genet Genomics 2012 39, 643e651. CHIR-99021 (CT99021) HCl purchased from Selleck.

  •  

    Smad linker phosphorylation involves GSK3 in human melanoma lines. Inhibition of Smad2 and Smad3 linker phosphorylation in the presence of the specific GSK3 specific inhibitor, CT99021. 24 hours post seeding, WM793 and 1205LU cells were serum-starved for about 16 hours, and incubated in the absence (2) or presence (+) of 2 uM of CT99021 for two hours. Immunoblots were performed as in A. p: Phosphorylated. S: Serine; T: Threonine.

    PLoS One 2012 7, e47312. CHIR-99021 (CT99021) HCl purchased from Selleck.

    A. Vero cells were pretreated with the indicated concentrations of CHIR-99021 for 1 h and then infected with PEDV (MOI = 1) for 8 or 10 h. Cell lysates were prepared and analyzed using Western blotting.

    PLoS One, 2016, 11(8):e0161508. CHIR-99021 (CT99021) HCl purchased from Selleck.

  • SKOV3/DDP cells were treated with a range of concentration of (A) CHIR-99021 or in combination with 20 µM cisplatin for 48 h.

    Exp Ther Med, 2016, 12(6):3851-3858. CHIR-99021 (CT99021) HCl purchased from Selleck.

     

    PANC1 cells were treated with GSK3 inhibitors SB216763 (SB 10µM or SB 20µM) or CHIR99021 (CHIR 5µM) for 72h.

    CHIR-99021 (CT99021) HCl purchased from Selleck.

  •  

     Activity of canonical WNT/ β -CATENIN signals induced by CHIR 99021 in the developing human lung. Human lung tissue explants obtained at 15 weeks of gestation were exposed to 0, 5 and 10 μM CHIR 99021 in vitro for 72 h. (A-B) Western blot analysis showed expression of β -CATENIN in human lung tissues. Results are presented as the mean ± sem of three independent experiments. *P < 0.05, **P < 0.01. (C) qRT-PCR analysis detected a dose-dependent increase in WNT signaling transcription factors and target genes following exposure to CHIR 99021. Results are presented as the mean ± sem of three independent experiments. *P < 0.05, **P < 0.01. (D-K) Histology examination showed obvious morphogenetic changes of the airway epithelial cells associated with CHIR 99021 treated lung tubes. Scale bars: D-G =100 μm; H-K = 20 μm.

    CHIR-99021 (CT99021) HCl purchased from Selleck.

Purity & Quality Control

Choose Selective GSK-3 Inhibitors

Biological Activity

Description CHIR-99021 HCl (CT99021) is hydrochloride of CHIR-99021, which is a GSK-3α/β inhibitor with IC50 of 10 nM/6.7 nM; CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs Cdc2 and ERK2.
Targets
GSK-3β [1]
(Cell-free assay)
GSK-3α [1]
(Cell-free assay)
Tie-2 [1]
(Cell-free assay)
FLT1 [1]
(Cell-free assay)
KDR [1]
(Cell-free assay)
6.7 nM 10 nM >5 μM >5 μM >5 μM
In vitro

CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs CDC2 and ERK2, as well as other protein kinases. Furthermore, CHIR-99021 shows only weak binding to a panel of 22 pharmacologically relevant receptors and little inhibitory activity against a panel of 23 nonkinase enzymes. CHIR-99021 induces the activation of glycogen synthase (GS) in insulin receptor-expressing CHO-IR cells with EC50 of 0.763 μM. [1] In addition to simulating the actions of insulin, inhibition of GSK-3 by CHIR-99021 (3 μM) increases free cytosolic β-catenin by 1.9-fold, mimicking the canonical Wnt signaling pathway in 3T3-L1 preadipocytes. During any of the first 3 days of differentiation, CHIR-99021 treatment inhibits the preadipocyte differentiation with IC50 of 0.3 μM by blocking induction of CCAAT/enhancer-binding protein α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ). [2] Unlike lithium chloride and AR-A014418, CHIR-99021 treatment does not reduce the viability of INS-1E cells even at high concentrations. Instead, CHIR-99021 robustly increases the rate of proliferation of INS-1E cells in a dose-dependent manner, and significantly inhibits INS-E cell death induced by high glucose and high palmitate in a concentration-dependent manner. CHIR-99021 promotes primary beta cell replication in isolated rat islets at concentrations as low as 1 μM, with 2-3 fold increase of cell replication at 5 μM of CHIR-99021 treatment. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
3T3-L1 NI\VPZlHfW6ldHnvckBCe3OjeR?= NXnKNmRJOyEQvF2= MljQOEBp NH;IXoNFVVOR NYXlSWdiUW6qaXLpeJMheHKnYXTpdI9kgXSnIHTp[oZmemWwdHnheIlwdg>? MVmxNlA2PTJyMB?=
insulin receptor–expressing CHO-IR NH7DTY1HfW6ldHnvckBCe3OjeR?= MWq1NFAhdk1? M3TCZ|MxKG2rbh?= MX3EUXNQ NYfUTItCSWO2aY\heIV{KEeueXPv[4VvKFO7boToZZNmKCiJUzm= MVOxNlYxPjR7Nx?=
H4IIE M4jvR2Z2dmO2aX;uJGF{e2G7 MWCxNEDPxE1? M3u2b|I1KGh? MY\EUXNQ Mn\YVoVofWyjdHXzJO6zNWOjdHXubY4h[WO2aY\peJkh[W6mIGTJVmUh[2:wdHHpcolv\yCycn;tc5RmeiCjY4Tpeol1gQ>? NV;vTZU{OTV|NUCxPVU>
Oct4/Sox2/Klf4-infected MEFs M3\Kc2Z2dmO2aX;uJGF{e2G7 MknVNVAh|ryP M4K4TlI5KGR? MoPnSG1UVw>? NYrhPIZvWHKxbX;0[ZMhVUWIczDy[ZBzd2e{YX3tbY5oKFS{YX7z[JVk\WRiYomgU4N1PCxiU3;4Nkwh[W6mIFvs[lQ> M1HpXVE6QDN7MEW1
Sca-1+ HPC M2\LOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXLzZo1iOiEQvF2= M4\Ib|Yh\A>? MVnEUXNQ NHv6RVdUgW6ncnfpd5Rq[2GubImgdJJwdW:2ZYOgV4NiNTFtwrDIVGMheHKxbHnm[ZJifGmxbjDhcoQh[2:ub375JIZwem2jdHnvckB1d2endHjldkB4cXSqIFXHSi=> NF[0eZMzOTZ{NEO0PC=>
HMC1.2 M4rndGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYr3OYhxOzBizszN NYTmXJlKPzJiaB?= MkjYSG1UVw>? NXO1OWU2WmWmdXPld{Bk\WyuIIP1dpZqfmGu MV6yNlA{QTNyMR?=
Endothelial cell(EC) NFHqOYpHfW6ldHnvckBCe3OjeR?= MkXtN{DPxE1? NYPGbHVnTE2VTx?= NFT0XphRem:vb4Tld{BmdmSxdHjlcIlidCClZXzsJEhGSyliZHnm[oVz\W62aXH0bY9v MoDyNlIxPTJyME[=
PANC1 M3vjZWFxd3C2b4Ppd{BCe3OjeR?= M3z1buKhPSEQvF2= NIC0e5U4OiCq MWLEUXNQ NYHreVZKXHKrZ3fldpMh[W5iYYDvdJRwfGmlIILld5BwdnOn NGjQVFAzOjJyMUG4Oi=>
INS-1E NUXQeHE6TnWwY4Tpc44hSXO|YYm= NXjTVGFkOi53IN88US=> M2\DXVI1KGh? NXr5cGVnTE2VTx?= NWTGeIVEUW6mdXPld{BiKHKnZIXjeIlwdiCxZjDOVFEhd3[ncnX4dJJme3Orb36= Mm[wNlI1Ojd5MES=
hiPSC-NPs NVqzS3NvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUSz5qCK|ryP NIK5TpM4OiCq NUG5WJI5TE2VTx?= NWfGVZlTUW6lcnXhd4V{KGOnbHygdJJwdGmoZYLheIlwdsLi MWOyNlY1OjZ6Nx?=
platelets MXHGeY5kfGmxbjDBd5NigQ>? MoHzNVAh|ryP MoXTNVUhdWmw MnfvSG1UVw>? MlPmTY5kemWjc3XzJJRpem:vYnnuMY1m\GmjdHXkJJBt[XSnbHX0JIFo\3KnZ3H0bY9vKGGwZDDBWHAhe2WlcnX0bY9v M2Wx[FI{OjN7OEe3
DN3 MnPhSpVv[3Srb36gRZN{[Xl? NHLpRpgyOCEQvF2= MlnRPVYhcA>? NEHqWHZFVVOR M2HMXWlv[3KnYYPld{Bk\WyuIHTleoVtd3CvZX70JIFv\CCycn;sbYZmemG2aX;u NUO3[4ZTOjN3Mk[5PFk>
embryonic stem cells (ESC) MWHGeY5kfGmxbjDBd5NigQ>? NXLhOGdyOyEEtV2= M2TNdFEyKGR? NIG1NHhFVVOR NFraSZRRem:vb4Tld{B{\WyoLYLlcoV4[Wxib3[gcoHEt3[nLYT5dIUhdW:3c3Wg[Y1jenmxbnnjJJN1\W1iY3XscJMhMEWVQzm= NWjMOoIyOjN6NEOzNVI>
H460 NXG3cmJ6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXq1JOK2VQ>? M{nZdVI1KGh? MUDEUXNQ M1;jXXBzd22xdHXzJINmdGxicILvcIln\XKjdHnvci=> NUCybWVZOjR|Nke2PFg>
H157 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYC1JOK2VQ>? M3n2VVI1KGh? NEHUflNFVVOR NXzGNoZLWHKxbX;0[ZMh[2WubDDwdo9tcW[ncnH0bY9v MkPLNlQ{Pjd4OEi=
H460 MUXBdI9xfG:|aYOgRZN{[Xl? MoLuOUDDvU1? NUfZR3dJOjRiaB?= M3jWeWROW09? M1fOVGF1fGWwdXH0[ZMh[2WubDDhdI9xfG:|aYO= NXnSWphlOjR|Nke2PFg>
H157 NHvENVNCeG:ydH;zbZMhSXO|YYm= Ml3rOUDDvU1? NEjOd5AzPCCq MkjMSG1UVw>? M4HZNmRw\XNibn;0JJNq\26rZnnjZY51dHliYX\m[YN1KGGyb4D0c5Nqew>? NF3BPWMzPDN4N{[4PC=>
A549 MoLkRZBweHSxc3nzJGF{e2G7 NYn6[Ih1PSEEtV2= M4DwelI1KGh? MnqySG1UVw>? NXLRb3hbUW6lcnXhd4V{KGOnbHygZZBweHSxc3nz M3njb|I1OzZ5Nki4
Neural progenitor cells MYDGeY5kfGmxbjDBd5NigQ>? NYjmUZZoOyEQvF2= MV[xNEBl M4LLb2ROW09? NGO5SWhKdmS3Y3XzJIdmdmW{YYTpc44hd2ZibnX1doFtKHC{b3flcol1d3JiY3XscJM> MkjDNlQ3OzhyM{S=
ES-D3 NFvrRm5EgXSxdH;4bYMhSXO|YYm= NUfMdJZiOTEEoN88US=> MXyzJIQ> MX3EUXNQ MXzJcoR2[2W|IH3v[IVz[XSnIHP5eI91d3irY3n0fUB4cXSqIFnDOVAhd2ZiND65JO69VQ>? M2PBeVI1Pzd7M{[1
ES-CCE M37md2N6fG:2b4jpZ{BCe3OjeR?= MXuxNOKh|ryP M1v6PFMh\A>? M324PWROW09? NX\BSpdxUW6mdXPld{Bud2SncnH0[UBkgXSxdH;4bYNqfHl? NYHsRXVtOjR5N{mzOlU>
ES-D3 NVvFTXA5TnWwY4Tpc44hSXO|YYm= M{mzVFXDqM7:TR?= MnyzN{Bl MluxSG1UVw>? NXK3eJM{W3S{b37ncJkhcW6mdXPld{B1cGViYXP0bZZqfHlib3[gW451N2KndHGtZ4F1\W6rbjDwZZRpf2G7 MorxNlQ4Pzl|NkW=
ES-CCE MVjGeY5kfGmxbjDBd5NigQ>? M3;X[VXDqM7:TR?= MW[zJIQ> NWDTOnJkTE2VTx?= NGCzNZBUfHKxbnfsfUBqdmS3Y3XzJJRp\SCjY4Tpeol1gSCxZjDXcpQw[mW2YT3jZZRmdmmwIIDheIh4[Xl? NIfrWpQzPDd5OUO2OS=>
SW982 MmXoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnLuTWM2OD1yLkKxOFE3KM7:TR?= MVfTRW5ITVJ?
GCT MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnyyTWM2OD1yLkOwOlczKM7:TR?= NVK2fVN7W0GQR1XS
769-P NXPQc49tT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV7JR|UxRTBwNUi0PVkh|ryP NVj4bmJXW0GQR1XS
LOXIMVI NWS3d3ZwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFHt[5FKSzVyPUCuOVg5OzJizszN MoLEV2FPT0WU
D-336MG M2HU[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2\0c2lEPTB;MD64OFYzKM7:TR?= MV3TRW5ITVJ?
S-117 NU\LfVhRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml;oTWM2OD1yLkmyNVMyKM7:TR?= M{TYV3NCVkeHUh?=
D-247MG NX3teG1oT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mm\CTWM2OD1yLkm0PFUzKM7:TR?= Ml\OV2FPT0WU
TYK-nu NWnSXZhIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYHJR|UxRTFwMEe5NVIh|ryP MYLTRW5ITVJ?
A498 NILKXIlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYfJR|UxRTFwM{C0Olkh|ryP NUTFOXNSW0GQR1XS
BCPAP M1P6Vmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVTOPJNlUUN3ME2xMlY3QDl6IN88US=> NELiPYFUSU6JRWK=
KYSE-180 MnvIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYn1WmhnUUN3ME2xMlczPzh6IN88US=> MoHnV2FPT0WU
MIA-PaCa-2 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NY\wdnQyUUN3ME2xMlc2QTd5IN88US=> Ml:xV2FPT0WU
HCC1395 NXH2RVVFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2XzRWlEPTB;MT63PFI4OyEQvF2= MoTlV2FPT0WU
RS4-11 NIKwR3FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIPzfYdKSzVyPUGuPVU1OTVizszN NEjS[GZUSU6JRWK=
NCI-H2122 M{K2bWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1foVGlEPTB;Mj6yO|Y5PCEQvF2= MomxV2FPT0WU
SNU-423 NVj5OYtQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3HoN2lEPTB;Mj60PVc5PyEQvF2= MWPTRW5ITVJ?
SK-LMS-1 NYqxSYYzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWLJR|UxRTJwNkSxNlgh|ryP MXvTRW5ITVJ?
RPMI-7951 NUfmWHRVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWjJR|UxRTJwN{CxPVMh|ryP MknUV2FPT0WU
COLO-829 NELUb2hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{fabmlEPTB;Mj63OlU1KM7:TR?= Mn;mV2FPT0WU
SCC-9 MkTZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWPJR|UxRTJwOUW0PFUh|ryP NGPqe3VUSU6JRWK=
A704 M1;tbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYrJR|UxRTNwMEixNkDPxE1? NF\HRZVUSU6JRWK=
HOP-62 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NW\mOHo3UUN3ME2zMlM3ODV2IN88US=> Ml:1V2FPT0WU
HCC1569 NWHi[VdCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFfSWHpKSzVyPUOuOFg{ODVizszN NF35TXZUSU6JRWK=
A172 M3rSd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NI\UU3FKSzVyPUOuO|U1QThizszN NGnCOpJUSU6JRWK=
MOLT-16 NXzifZV2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWrJR|UxRTNwOEi1OVYh|ryP MkK5V2FPT0WU
TE-15 Mm\RS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYPxUVduUUN3ME2zMlk2OzR|IN88US=> MXfTRW5ITVJ?
OE19 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmjkTWM2OD1|Lkm2OFUyKM7:TR?= NX;qcHVkW0GQR1XS
MHH-ES-1 M1rZe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlPITWM2OD12LkC0OVQ2KM7:TR?= M{e1ZnNCVkeHUh?=
NKM-1 M{HyW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX;UR5JLUUN3ME20MlE{QTdzIN88US=> M4XZ[nNCVkeHUh?=
RCC10RGB MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2PBVGlEPTB;ND6yPVQ4QSEQvF2= NW\jTFZqW0GQR1XS
BxPC-3 NVm1WogyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmDHTWM2OD12LkewN|kyKM7:TR?= NHzRUmhUSU6JRWK=
ALL-PO M4W4V2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2TtbGlEPTB;ND64N|g2QCEQvF2= MX;TRW5ITVJ?
SK-OV-3 NHrCSHlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIPUbFZKSzVyPUSuPVk{PCEQvF2= MoDGV2FPT0WU
SW1710 MlfxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHvKeGFKSzVyPUWuOFI1PTZizszN M1HJTHNCVkeHUh?=
NCI-H2452 NEnXZldIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlXuTWM2OD13LkS1OlI5KM7:TR?= MXTTRW5ITVJ?
D-392MG NWjtVpNYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkWyTWM2OD13LkS5NVQ2KM7:TR?= MV7TRW5ITVJ?
LB2518-MEL MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF;lTJBKSzVyPUWuO|I3OjdizszN M370W3NCVkeHUh?=
BL-41 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFXScFhKSzVyPUWuPFEzPjZizszN NV3WcFFOW0GQR1XS
WM-115 NWL0WFhFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXPJR|UxRTVwOEG1NFkh|ryP MWXTRW5ITVJ?
SK-MEL-2 M{LWTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnPBTWM2OD14LkO4N|Q1KM7:TR?= MYHTRW5ITVJ?
CGTH-W-1 NHnhcolIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHW3O3NKSzVyPU[uOFE4KM7:TR?= MUfTRW5ITVJ?
BB65-RCC NWr6e4JtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH60e41KSzVyPU[uOVMyQTVizszN MWPTRW5ITVJ?
CAL-85-1 Ml;6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{\HSGlEPTB;Nj62NFk{PiEQvF2= NVXJcoh3W0GQR1XS
RT-112 NGHHdJNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEDVc5hKSzVyPUeuNVY1PTZizszN M{XVdXNCVkeHUh?=
MC116 M12xdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVfJR|UxRTdwMki2PVEh|ryP NEm3eFZUSU6JRWK=
G-361 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYfCfJVLUUN3ME23MlQ3PTZzIN88US=> NFXBVoZUSU6JRWK=
SW1417 NHe5coVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MULJR|UxRTdwNkS1PFQh|ryP NV\zWGN3W0GQR1XS
MV-4-11 MmraS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4qyWmlEPTB;OD61OFMxPCEQvF2= MYrTRW5ITVJ?
CAL-72 NYnBXmN5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NID1[YVKSzVyPUiuO|YyOTNizszN MnXoV2FPT0WU
MKN28 NVf5ZnVwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWrJR|UxRThwOEi1NVYh|ryP M3\Jb3NCVkeHUh?=
MEG-01 Moq1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NW\ab241UUN3ME24Mlk6ODR|IN88US=> NHTJb5VUSU6JRWK=
ACHN MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn3uTWM2OD17LkOwOVQ3KM7:TR?= MoO2V2FPT0WU
NCI-H1755 NYrxOI17T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYXJR|UxRTlwM{m5PFYh|ryP MlP0V2FPT0WU
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BHY MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIjMfVZKSzVyPUOyN{4yPzVizszN MWTTRW5ITVJ?
OCUB-M MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV\pUVBvUUN3ME2zNlMvPDN2IN88US=> MV\TRW5ITVJ?
HCC1954 M2jIRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXjJR|UxRTN{Mz61PFIh|ryP MVzTRW5ITVJ?
SK-MEL-24 NHq5bFhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1nUeGlEPTB;M{K0MlEyOSEQvF2= MnPhV2FPT0WU
NTERA-S-cl-D1 M17qOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYX4[no6UUN3ME2zNlYvPDh7IN88US=> M1\Ue3NCVkeHUh?=
SK-MEL-30 Ml;xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2HRc2lEPTB;M{K2Mlc{QCEQvF2= NUfod4p1W0GQR1XS
LS-123 MnnoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkHtTWM2OD1|MkiuNVY1KM7:TR?= NXnmVpk6W0GQR1XS
SF268 NVLkbWVYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVXJR|UxRTN|MD6yOlUh|ryP NF7GNoNUSU6JRWK=
BT-474 NYf1[4FzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoTGTWM2OD1|M{KuOFE5KM7:TR?= MWrTRW5ITVJ?
NCI-H1522 MmfES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYLJR|UxRTN|ND6zN|kh|ryP MYnTRW5ITVJ?
LC-2-ad MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHTLNpdKSzVyPUOzOk42QTFizszN MYHTRW5ITVJ?
LAN-6 NGe5NZJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVPJR|UxRTN|Nj65O|ch|ryP MkDKV2FPT0WU
ARH-77 NFvTR4VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2TocmlEPTB;M{O3MlMzKM7:TR?= NHPUVYRUSU6JRWK=
HuO9 MkLoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVLJR|UxRTN|Nz60O{DPxE1? NVfRU|dXW0GQR1XS
MLMA NEK5V4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFPnSnJKSzVyPUOzPE4yPTVizszN NH7JR4lUSU6JRWK=
Raji MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX\JOYlOUUN3ME2zN|gvOzhizszN M1XvfnNCVkeHUh?=
GT3TKB Ml\yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWLkN4szUUN3ME2zN|gvPDF{IN88US=> NEfxRXhUSU6JRWK=
NCI-H441 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHPW[GdKSzVyPUO0NU41OThizszN M3SxbHNCVkeHUh?=
NCI-H2030 NHLuUmlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWLJR|UxRTN2MT62NFkh|ryP M2PtV3NCVkeHUh?=
VM-CUB-1 NHHYS2ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXSxNGJkUUN3ME2zOFIvPjN4IN88US=> NWn5ZVVEW0GQR1XS
KP-4 NFW5UHdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1;JVGlEPTB;M{SzMlc{OyEQvF2= MV7TRW5ITVJ?
PF-382 MmexS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1uwTGlEPTB;M{S0MlQzOiEQvF2= MXjTRW5ITVJ?
NB13 NHfKcnVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{DC[WlEPTB;M{S1MlU5KM7:TR?= MUjTRW5ITVJ?
D-502MG NHXxZWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYLJR|UxRTN2NT64NVIh|ryP MlnWV2FPT0WU
CAL-54 Ml;KS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV3LTYF{UUN3ME2zOFUvQDd7IN88US=> MV7TRW5ITVJ?
DOHH-2 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGXvV5ZKSzVyPUO0Ok43PSEQvF2= MXHTRW5ITVJ?
SK-PN-DW M2nXdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXzLV2syUUN3ME2zOFcvPDJ4IN88US=> MmO2V2FPT0WU
NCI-H720 MmHIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3HHWGlEPTB;M{S5Mlg1PiEQvF2= NGDqXWVUSU6JRWK=
SW1990 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmPwTWM2OD1|NUGuNlgh|ryP NILmfmdUSU6JRWK=
NCI-H2196 NXf5XWF[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NE\QZXNKSzVyPUO1Nk43OjRizszN NHzTOJNUSU6JRWK=
P30-OHK MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1P6fGlEPTB;M{WzMlA3OyEQvF2= M2X4UXNCVkeHUh?=
NCI-H358 NIO5XmtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFuyeoZKSzVyPUO1OU4{PTZizszN NXHmR4hHW0GQR1XS
CW-2 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4THUWlEPTB;M{W2MlUzQSEQvF2= NH3QToNUSU6JRWK=
COLO-678 Mk\4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NELkbHpKSzVyPUO1O{45PjNizszN M2XVU3NCVkeHUh?=
NCI-H2126 NEnXfYFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmPjTWM2OD1|NUiuOVIyKM7:TR?= NXvSfo1VW0GQR1XS
DMS-53 MkO2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYDJR|UxRTN3OD63NVEh|ryP NWroRm9kW0GQR1XS
NCI-H1838 NFfNcpNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXq5XY5[UUN3ME2zOVkvQTh5IN88US=> MVXTRW5ITVJ?
SJSA-1 NYSwOZBoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUfjWm1vUUN3ME2zOlAvQTN7IN88US=> MXTTRW5ITVJ?
ChaGo-K-1 M2LDbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF\ETplKSzVyPUO2N{46OjRizszN NIn2XoVUSU6JRWK=
IST-SL2 M3Lkemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHTSSm9KSzVyPUO3Nk46OjZizszN NXrsTVFqW0GQR1XS
U-266 NEO2T5lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVvJR|UxRTN5Mz6xN|Ih|ryP NYXlNm1YW0GQR1XS
P31-FUJ M3rwZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVvJR|UxRTN5Nj65OlQh|ryP NIXR[lZUSU6JRWK=
HCC2157 NYPIepN6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUnPcJplUUN3ME2zO|gvOTZzIN88US=> MV3TRW5ITVJ?
COR-L88 M2jvN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXTJR|UxRTN6Mz62O|ch|ryP NH;ENG5USU6JRWK=
NCI-H596 MlHES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV3ZSplJUUN3ME2zPFUvOzRizszN M2PyZXNCVkeHUh?=
GOTO Mli5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml2zTWM2OD1|OUCuOlQyKM7:TR?= MXPTRW5ITVJ?
NCI-H719 NIfZdZlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVv3bXE3UUN3ME2zPVEvQDl|IN88US=> M3m3OHNCVkeHUh?=
MN-60 NICwNVJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1nDUGlEPTB;M{mzMlk1QCEQvF2= NUP0TolUW0GQR1XS
IST-SL1 M2\xT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUT0WYd5UUN3ME2zPVQvPDB4IN88US=> MV\TRW5ITVJ?
TE-6 NHvhPW5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHLIbJpKSzVyPUO5O{4zPTRizszN MXnTRW5ITVJ?
SW962 M4DGXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXHJR|UxRTN7OD62PUDPxE1? M2WzRXNCVkeHUh?=
FADU MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3;NeWlEPTB;NECzMlA5OyEQvF2= M2TVUHNCVkeHUh?=
ECC4 M{WwcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWHJR|UxRTRyOD60OVgh|ryP NWfUWIVNW0GQR1XS
MES-SA NY[yU|huT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV\JR|UxRTRyOT65PEDPxE1? MWHTRW5ITVJ?
NCI-H716 M3HsOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlrYTWM2OD12MUCuNFc2KM7:TR?= MWTTRW5ITVJ?
GR-ST NF\IVGNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYPJR|UxRTRzMD60PVch|ryP MkfvV2FPT0WU
KY821 Mo\kS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUK4WHVKUUN3ME20NVQvODh4IN88US=> MXjTRW5ITVJ?
NCI-H2141 MkW3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2nMemlEPTB;NEK0MlM5QCEQvF2= NE\xfIVUSU6JRWK=
MDA-MB-134-VI M4\oXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MojHTWM2OD12M{GuNFM{KM7:TR?= MWPTRW5ITVJ?
RH-1 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVXJR|UxRTR|NT6wPUDPxE1? M3;USnNCVkeHUh?=
AM-38 MkDMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2TyVmlEPTB;NEO4MlU1KM7:TR?= MonoV2FPT0WU
BL-70 MoDsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVHJR|UxRTR|OT60Nlkh|ryP NUXnZWJmW0GQR1XS
COLO-684 M3nDN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkHuTWM2OD12NEiuPFg5KM7:TR?= M{X2bnNCVkeHUh?=
DMS-79 NGfUboNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4fTOmlEPTB;NES5MlY5KM7:TR?= NYO5VmxSW0GQR1XS
EW-1 MoLxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEL3[3ZKSzVyPUS1Nk45QDhizszN Ml;WV2FPT0WU
MS-1 MoPRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnPhTWM2OD12NUSuN|g2KM7:TR?= NV7PU5V2W0GQR1XS
A4-Fuk MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVjCfm5UUUN3ME20OVQvPTB5IN88US=> NVfZcGRpW0GQR1XS
TALL-1 NWfhcm5[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{jjOmlEPTB;NEW1MlI6PyEQvF2= MWnTRW5ITVJ?
SCLC-21H MkfIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVPJR|UxRTR3NT62PVkh|ryP MmTCV2FPT0WU
KMS-12-PE M{\Ud2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3X0bGlEPTB;NEW2MlgzOiEQvF2= NYW4XWNHW0GQR1XS
TE-441-T M4PQNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXPJR|UxRTR3Nz6xPFYh|ryP Mn\WV2FPT0WU
A3-KAW MlTyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{jXc2lEPTB;NE[wMlg1OSEQvF2= MkCxV2FPT0WU
NCI-H1092 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn\VTWM2OD12NkOuPVQ{KM7:TR?= NUHQRYFpW0GQR1XS
COR-L279 MlPyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mke3TWM2OD12NkSuPFQ4KM7:TR?= M{XVO3NCVkeHUh?=
NCI-SNU-5 M3nHN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkLqTWM2OD12N{KuNlgh|ryP NWH4[owyW0GQR1XS
JAR NIrRTZZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIXRUHFKSzVyPUS3OE44QDhizszN M4Gzb3NCVkeHUh?=
NCI-H446 Mo\1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmDVTWM2OD12N{euNFgh|ryP NW\KPVFXW0GQR1XS
HDLM-2 NHroO3JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnLETWM2OD12OECuPFY6KM7:TR?= NYXkcVN[W0GQR1XS
D-283MED MlzhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmPVTWM2OD12OEiuPVI1KM7:TR?= Mk\qV2FPT0WU
NCI-H1395 NGD3Z3ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NInL[4JKSzVyPUS4PU4xODRizszN MWLTRW5ITVJ?
RPMI-6666 NHvwcWVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1nSbWlEPTB;NEmxMlM6QCEQvF2= MnrpV2FPT0WU
CPC-N MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1vvWGlEPTB;NUCyMlA3OyEQvF2= MUfTRW5ITVJ?
EC-GI-10 NVHxXWtlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXf0bmxNUUN3ME21NFIvOTZ7IN88US=> NVvzfFQ4W0GQR1XS
CA46 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4POXGlEPTB;NUC0MlQxPyEQvF2= M3rpdnNCVkeHUh?=
EHEB MljxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3[3cmlEPTB;NUC3MlIxQCEQvF2= M1;TSnNCVkeHUh?=
RPMI-8402 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEPJeXVKSzVyPUWxNE4xOjFizszN Moi5V2FPT0WU
DU-4475 MoXlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHznSXpKSzVyPUWxNU4yODFizszN NX6wTXJMW0GQR1XS
P12-ICHIKAWA NUDUcm52T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWjJR|UxRTVzMT60NVYh|ryP NX[0NXp2W0GQR1XS
NCI-H1694 M1jVd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXTBS25VUUN3ME21NVIvPDN7IN88US=> NEfTUpZUSU6JRWK=
NCI-H2171 MkfMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVf4WGU{UUN3ME21NVIvPzB3IN88US=> NGXmT3VUSU6JRWK=
Mo-T M{TGZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHrHc3hKSzVyPUWxOU4yOTZizszN MnLXV2FPT0WU
HL-60 MkXBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2HRR2lEPTB;NUKyMlkzPCEQvF2= MYDTRW5ITVJ?
NCI-H1436 NYqw[2hCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVrJR|UxRTV{Mz6wOlQh|ryP NFvqTFZUSU6JRWK=
NCI-H510A M3HudWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYjJR|UxRTV{ND62PVYh|ryP MUHTRW5ITVJ?
NCI-H2081 NHzWe45Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUP4ZpFlUUN3ME21NlUvPTF|IN88US=> NVe5b4NyW0GQR1XS
NB5 NIjwfG5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIrSSnBKSzVyPUWyOk41OyEQvF2= M1f5V3NCVkeHUh?=
NCI-H2227 Mo\VS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUDJR|UxRTV|MD6yOVQh|ryP MXzTRW5ITVJ?
HSC-4 MoPtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnG5TWM2OD13M{muNlk{KM7:TR?= M3K3UHNCVkeHUh?=
RL Mn\iS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVrrNYV4UUN3ME21OFIvPDR{IN88US=> MYjTRW5ITVJ?
EVSA-T NH7NcHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mme4TWM2OD13NEOuOlg1KM7:TR?= M2rX[XNCVkeHUh?=
NCI-H889 NXTwXG1OT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXnyU4QxUUN3ME21OFcvOjNizszN NEXMPVBUSU6JRWK=
COLO-320-HSR NHHoSXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYHJR|UxRTV3MT6zOUDPxE1? M1u4S3NCVkeHUh?=
EW-11 M4r1bmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYHJR|UxRTV3Mj6zPVkh|ryP NH\OTXlUSU6JRWK=
UACC-812 M{XUXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUe3cndUUUN3ME21OVkvOzlzIN88US=> MnTFV2FPT0WU
CAL-148 NYLQNY5XT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUS2XFk5UUN3ME21OlUvODh7IN88US=> NIDrfmtUSU6JRWK=
K052 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1\ENGlEPTB;NUe5MlIyOyEQvF2= NV;kcmw5W0GQR1XS
L-428 NVP4bY9CT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHO3NlFKSzVyPU[wNE43PzFizszN MoLqV2FPT0WU
LP-1 NFzIboRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3TycWlEPTB;NkCzMlU5PyEQvF2= NHvLc4xUSU6JRWK=
ML-2 NXrMO3U3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEnJNpVKSzVyPU[wOk42PzlizszN MYnTRW5ITVJ?
KARPAS-45 M3PHWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVHJR|UxRTZzOD6yNlEh|ryP NYK3V4ttW0GQR1XS
HT MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3\VRmlEPTB;NkKyMlc5PSEQvF2= M4T0dnNCVkeHUh?=
NCI-H226 M3n0VWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIn0ZWNKSzVyPU[yPE4{PTNizszN NV3DUlJUW0GQR1XS
L-363 NFXkeolIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX3JR|UxRTZ2OD6wOlQh|ryP MXLTRW5ITVJ?
IM-9 MmDUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHnReIZKSzVyPU[1NU42PDFizszN MmH5V2FPT0WU
TUR NW\yem1oT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHHYcpVKSzVyPU[3NU4{OjJizszN MWDTRW5ITVJ?
NCI-SNU-16 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlvJTWM2OD14OECuOFYzKM7:TR?= NYjRRWwxW0GQR1XS
NCI-SNU-1 NVf5OpNZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M12ycGlEPTB;NkmwMlE4OiEQvF2= NInrOmhUSU6JRWK=
U-87-MG NGTybZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mm\TTWM2OD14OUSuOVg2KM7:TR?= M4XZT3NCVkeHUh?=
KM-H2 MojwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYfBd5FHUUN3ME22PVkvODV7IN88US=> Mke2V2FPT0WU
DEL NHHT[XZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoDGTWM2OD15MECuPFM5KM7:TR?= NXvmR4k5W0GQR1XS
KARPAS-422 NH7BWWpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGPFe2FKSzVyPUe4PU4{OTVizszN NGTacFlUSU6JRWK=
BC-3 NXP6ZZhzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVjzUJpyUUN3ME24NFUvOjB|IN88US=> NGraU4NUSU6JRWK=
NCI-H1155 NFi3e4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mki0TWM2OD16MUiuNFU6KM7:TR?= MUXTRW5ITVJ?
NCI-H1581 NIfsc4hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVWyRmNqUUN3ME25NFAvOjNzIN88US=> Ml:yV2FPT0WU

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In vivo Oral administration of CHIR-99021 at 30 mg/kg enhances glucose metabolism in a rodent model of type 2 diabetes, with a maximal plasma glucose reduction of nearly 150 mg/dl 3-4 hours after administration, while plasma insulin remains at or below control levels. Oral administration of CHIR-99021 at 16 or 48 mg/kg 1 hour before oral glucose challenges in ZDF rats significantly improves glucose tolerance with 14% and 33% reduction in plasma glucose at 16 mg/kg and 48 mg/kg, respectively, and the higher dose of CHIR-99021 also reduces hyperglycemia before the oral glucose challenge. [1] Administration of CHIR-99021 significantly augments hematopoietic repopulation in recipient mice transplanted with mouse or human hematopoietic stem cells (HSCs), suggesting that GSK-3 is a specific modulator of HSC activity. [4]

Protocol

Kinase Assay:

[1]

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Cell-free kinase assays:

Polypropylene 96-well plates are filled with 300 μL/well buffer (50 mM tris HCl, 10 mM MgCl2, 1 mM EGTA, 1 mM dithiothreitol, 25 mM β-glycerophosphate, 1 mM NaF, 0.01% BSA, pH 7.5) containing 27 nM GSK-3α or 29 nM GSK-3β, and 0.5 μM biotin-CREB peptide substrate. Various concentrations of CHIR-99021 are added in 3.5 μL of DMSO, followed by 50 μL of ATP stock to yield a final concentration of 1 μM ATP in all cell-free assays. After incubation, triplicate 100-μL aliquots are transferred to Combiplate eight plates containing 100 μL/well 50 μM ATP and 20 mM EDTA. After 1 hour, the wells are rinsed five times with PBS, filled with 200 μL of scintillation fluid, sealed, left 30 minutes, and counted in a scintillation counter. All steps are performed at room temperature.
Cell Research:

[3]

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  • Cell lines: INS-1E
  • Concentrations: Dissolved in DMSO, final concentrations ~ 20 μM
  • Incubation Time: 1, or 4 days
  • Method:

    Cells are maintained for 24 hours in starvation medium (culture medium with only 5 mM glucose, 1% fetal calf serum). Then cells are exposed to various concentrations of CHIR-99021 for 1, or 4 days. Cell number is measured by staining of cellular DNA with CyQuant dye, which becomes fluorescent when bound to DNA. Fluorescence is measured after 30 minutes of incubation using the FLUOstar Optima reader. Cell replication is determined by BrdUrd incorporation. BrdUrd labeling solution is added to the medium for the last 4 hours before the cells are fixed using FixDenat solution and incubated with monoclonal anti-BrdUrd-POD antibodies. After substrate solution is added to each well, the light emission is measured in a microplate luminometer using the Analyst HT detection system.


    (Only for Reference)
Animal Research:

[1]

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  • Animal Models: Female db/db mice or male ZDF rats with type 2 diabetes
  • Formulation: Formulated as solutions in 20 mM citrate-buffered 15% Captisol or as fine suspensions in 0.5% carboxymethylcellulose.
  • Dosages: ~48 mg/kg
  • Administration: Orally
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 93 mg/mL (185.33 mM)
Ethanol 2 mg/mL (3.98 mM)
Water slightly soluble or insoluble
In vivo Add solvents individually and in order:
5% DMSO+30% PEG 300+ddH2O
24mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 501.8
Formula

C22H18Cl2N8.HCl

CAS No. 252917-06-9 (free base)
Storage powder
in solvent
Synonyms N/A

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Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID