CHIR-99021 (CT99021) HCl

Catalog No.S2924

CHIR-99021 (CT99021) HCl Chemical Structure

Molecular Weight(MW): 501.8

CHIR-99021 HCl (CT99021) is hydrochloride of CHIR-99021, which is a GSK-3α/β inhibitor with IC50 of 10 nM/6.7 nM; CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs Cdc2 and ERK2.

Size Price Stock Quantity  
In DMSO USD 340 In stock
USD 90 In stock
USD 170 In stock
USD 570 In stock
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Cited by 70 Publications

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  • Differentiation induced by treatment with the Gsk3 inhibitor in hPSCs is β-catenin dependent. H9-7TGP cells were treated with 12 uM CH in mTeSR1 for 4 d. Immunofluorescent staining for Oct4, Isl1, and Nkx2.5 was compared with GFP expression. (Scale bars, 50 uM.)

    Proc Natl Acad Sci U S A 2012 109(27):E1848-57. CHIR-99021 (CT99021) HCl purchased from Selleck.

    Gpc4 regulates ESC response to Wnt/β-catenin signaling.  (E) Quantitative analysis of control and Gpc4-mutant beating foci exposed to the GSK3 inhibitor, CHIR 99021 (1 and 3 μM, from day 0 to day 5). Analysis was done at different time points. Gpc4- mutant cells treated with CHIR 99021 behave as controls (mean±SEM; n=2).

    Stem Cells 2012 30, 1863-1874. CHIR-99021 (CT99021) HCl purchased from Selleck.

  • Inhibition of GSK3 activity induces an autophagic response in human pancreatic cancer cells. PANC1 cells were treated for 24 h with DMSO, CHIR99021 (CHIR; 5 uM) or the mTOR inhibitor Torin1 (250 nM). Autophagosome (punctate LC3B) (D) and lysosome (E) labeling was visualized with a Zeiss LSM700 confocal microscope. Images were acquired with a 20x (D) or 63x (E) objective. Nuclei were stained with DAPI. Scale bars, 20 祄.

    J Biol Chem 2015 290(9), 5592-605 . CHIR-99021 (CT99021) HCl purchased from Selleck.

     

    Characterization of rES cells. A: image of normal rES cell colonies on feeder layers. B: rES colonies were positive for AP staining. CeE: rES colonies readily expressed pluripotent markers, Sox2 (C), Oct4 (D), and SSEA-1 (E). Blue, DAPI. Scale bars: 100 um.

    J Genet Genomics 2012 39, 643e651. CHIR-99021 (CT99021) HCl purchased from Selleck.

  •  

    Smad linker phosphorylation involves GSK3 in human melanoma lines. Inhibition of Smad2 and Smad3 linker phosphorylation in the presence of the specific GSK3 specific inhibitor, CT99021. 24 hours post seeding, WM793 and 1205LU cells were serum-starved for about 16 hours, and incubated in the absence (2) or presence (+) of 2 uM of CT99021 for two hours. Immunoblots were performed as in A. p: Phosphorylated. S: Serine; T: Threonine.

    PLoS One 2012 7, e47312. CHIR-99021 (CT99021) HCl purchased from Selleck.

    A. Vero cells were pretreated with the indicated concentrations of CHIR-99021 for 1 h and then infected with PEDV (MOI = 1) for 8 or 10 h. Cell lysates were prepared and analyzed using Western blotting.

    PLoS One, 2016, 11(8):e0161508. CHIR-99021 (CT99021) HCl purchased from Selleck.

  • SKOV3/DDP cells were treated with a range of concentration of (A) CHIR-99021 or in combination with 20 µM cisplatin for 48 h.

    Exp Ther Med, 2016, 12(6):3851-3858. CHIR-99021 (CT99021) HCl purchased from Selleck.

     

    PANC1 cells were treated with GSK3 inhibitors SB216763 (SB 10µM or SB 20µM) or CHIR99021 (CHIR 5µM) for 72h.

    CHIR-99021 (CT99021) HCl purchased from Selleck.

  •  

     Activity of canonical WNT/ β -CATENIN signals induced by CHIR 99021 in the developing human lung. Human lung tissue explants obtained at 15 weeks of gestation were exposed to 0, 5 and 10 μM CHIR 99021 in vitro for 72 h. (A-B) Western blot analysis showed expression of β -CATENIN in human lung tissues. Results are presented as the mean ± sem of three independent experiments. *P < 0.05, **P < 0.01. (C) qRT-PCR analysis detected a dose-dependent increase in WNT signaling transcription factors and target genes following exposure to CHIR 99021. Results are presented as the mean ± sem of three independent experiments. *P < 0.05, **P < 0.01. (D-K) Histology examination showed obvious morphogenetic changes of the airway epithelial cells associated with CHIR 99021 treated lung tubes. Scale bars: D-G =100 μm; H-K = 20 μm.

    CHIR-99021 (CT99021) HCl purchased from Selleck.

Purity & Quality Control

Choose Selective GSK-3 Inhibitors

Biological Activity

Description CHIR-99021 HCl (CT99021) is hydrochloride of CHIR-99021, which is a GSK-3α/β inhibitor with IC50 of 10 nM/6.7 nM; CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs Cdc2 and ERK2.
Targets
GSK-3β [1]
(Cell-free assay)
GSK-3α [1]
(Cell-free assay)
6.7 nM 10 nM
In vitro

CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs CDC2 and ERK2, as well as other protein kinases. Furthermore, CHIR-99021 shows only weak binding to a panel of 22 pharmacologically relevant receptors and little inhibitory activity against a panel of 23 nonkinase enzymes. CHIR-99021 induces the activation of glycogen synthase (GS) in insulin receptor-expressing CHO-IR cells with EC50 of 0.763 μM. [1] In addition to simulating the actions of insulin, inhibition of GSK-3 by CHIR-99021 (3 μM) increases free cytosolic β-catenin by 1.9-fold, mimicking the canonical Wnt signaling pathway in 3T3-L1 preadipocytes. During any of the first 3 days of differentiation, CHIR-99021 treatment inhibits the preadipocyte differentiation with IC50 of 0.3 μM by blocking induction of CCAAT/enhancer-binding protein α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ). [2] Unlike lithium chloride and AR-A014418, CHIR-99021 treatment does not reduce the viability of INS-1E cells even at high concentrations. Instead, CHIR-99021 robustly increases the rate of proliferation of INS-1E cells in a dose-dependent manner, and significantly inhibits INS-E cell death induced by high glucose and high palmitate in a concentration-dependent manner. CHIR-99021 promotes primary beta cell replication in isolated rat islets at concentrations as low as 1 μM, with 2-3 fold increase of cell replication at 5 μM of CHIR-99021 treatment. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
3T3-L1 M1PDWGZ2dmO2aX;uJGF{e2G7 M4nzWFMh|ryP MXi0JIg> M4r0SGROW09? MnLMTY5pcWKrdIOgdJJm[WSrcH;jfZRmKGSrZn\ldoVvfGmjdHnvci=> NUPwe4lHOTJyNUWyNFA>
insulin receptor–expressing CHO-IR M1rSfmZ2dmO2aX;uJGF{e2G7 NXHnbFhGPTByIH7N Mli4N|AhdWmw MY\EUXNQ M1;NU2FkfGm4YYTld{BIdHmlb3flckBUgW62aHHz[UApT1Nr NYXUSoZMOTJ4ME[0PVc>
H4IIE NH;NfWNHfW6ldHnvckBCe3OjeR?= NV;aVXdrOTBizszN NYTtbYdzOjRiaB?= MmTZSG1UVw>? NH3MZmhT\We3bHH0[ZMh|rJvY3H0[Y5qdiCjY4Tpeol1gSCjbnSgWGlTTSClb370ZYlvcW6pIIDyc41wfGW{IHHjeIl3cXS7 M2Pxd|E2OzVyMUm1
Oct4/Sox2/Klf4-infected MEFs MWrGeY5kfGmxbjDBd5NigQ>? NFjjTWgyOCEQvF2= MomzNlgh\A>? NU\sVJlSTE2VTx?= MnGxVJJwdW:2ZYOgUWVHeyC{ZYDyc4dz[W2vaX7nJHRz[W6|ZIXj[YQh[nliT3P0OEwhW2:6MjygZY5lKEuuZkS= MWGxPVg{QTB3NR?=
Sca-1+ HPC NGDHenpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{XBXVIh|ryP MmeyOkBl M{WwRWROW09? MWjTfY5memerc4TpZ4FtdHlicILvcY91\XNiU3PhMVEsyqCKUFOgdJJwdGmoZYLheIlwdiCjbnSgZ49td267IH\vdo1ifGmxbjD0c4dmfGincjD3bZRpKEWJRh?= M2HoSFIyPjJ2M{S4
HMC1.2 MkDzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV7zdVdCOzBizszN M1XET|czKGh? NHHyPIVFVVOR MofuVoVlfWOnczDj[YxtKHO3co\peoFt MXSyNlA{QTNyMR?=
Endothelial cell(EC) NX35[25nTnWwY4Tpc44hSXO|YYm= MWOzJO69VQ>? NHLEfFBFVVOR MmjNVJJwdW:2ZYOg[Y5ld3SqZXzpZYwh[2WubDCoSWMqKGSrZn\ldoVvfGmjdHnvci=> NW\3[nRZOjJyNUKwNFY>
PANC1 NWi5PGQzSXCxcITvd4l{KEG|c3H5 MWhCpFUh|ryP M1v0SVczKGh? MlzsSG1UVw>? MUDUdolo\2W{czDhckBieG:ydH;0bYMhemW|cH;ud4U> MkfPNlIzODFzOE[=
INS-1E NWjpUI85TnWwY4Tpc44hSXO|YYm= NHz0b4QzNjVizszN NUDW[4JEOjRiaB?= NGPpRWVFVVOR MlT6TY5lfWOnczDhJJJm\HWldHnvckBw\iCQUEGgc5ZmemW6cILld5Nqd25? MYWyNlQzPzdyNB?=
hiPSC-NPs Mn7lS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4ixXFPjiIoQvF2= M3P0c|czKGh? M17XOmROW09? M4\rcWlv[3KnYYPld{Bk\WyuIIDyc4xq\mW{YYTpc47DqA>? MXSyNlY1OjZ6Nx?=
platelets NH\yc3FHfW6ldHnvckBCe3OjeR?= M374PFExKM7:TR?= M2XC[lE2KG2rbh?= NUfEbWk1TE2VTx?= MWXJcoNz\WG|ZYOgeIhzd22kaX6tcYVlcWG2ZXSgdIxifGWuZYSgZYdoemWpYYTpc44h[W6mIFHUVEB{\WO{ZYTpc44> M3;sfVI{OjN7OEe3
DN3 NH\PfWtHfW6ldHnvckBCe3OjeR?= MmnTNVAh|ryP NES1[Xk6PiCq M13WZWROW09? NH3OU5FKdmO{ZXHz[ZMh[2WubDDk[ZZmdG:ybXXueEBidmRicILvcIln\XKjdHnvci=> M2K1SVI{PTJ4OUi5
embryonic stem cells (ESC) M1fkTWZ2dmO2aX;uJGF{e2G7 NHfPSG0{KML3TR?= NF7iT2gyOSCm NHrH[YJFVVOR MUnQdo9ud3SnczDz[YxnNXKnbnX3ZYwhd2ZibnJDs5ZmNXS7cHWgcY92e2ViZX3idplwdmmlIIP0[Y0h[2WubIOgLGVUSyl? M1jkcVI{QDR|M{Gy
H460 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVO1JOK2VQ>? NGry[2MzPCCq Mon5SG1UVw>? MWnQdo9ud3SnczDj[YxtKHC{b3zp[oVz[XSrb36= MVKyOFM3PzZ6OB?=
H157 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVXqfHp[PSEEtV2= NITLZWUzPCCq M{PSO2ROW09? NETMXm9Rem:vb4Tld{Bk\WyuIIDyc4xq\mW{YYTpc44> MUCyOFM3PzZ6OB?=
H460 NEHwSW1CeG:ydH;zbZMhSXO|YYm= MlLrOUDDvU1? NX7WbXBpOjRiaB?= M3TBZWROW09? M3L1RWF1fGWwdXH0[ZMh[2WubDDhdI9xfG:|aYO= M36wOVI1OzZ5Nki4
H157 NWP4Wm5FSXCxcITvd4l{KEG|c3H5 NX;CUHBvPSEEtV2= MXWyOEBp M{nHRmROW09? NEjnTIFFd2W|IH7veEB{cWewaX\pZ4FvfGy7IHHm[oVkfCCjcH;weI9{cXN? NWC5bXdEOjR|Nke2PFg>
A549 Mo\SRZBweHSxc3nzJGF{e2G7 NHXDOWQ2KML3TR?= M1K2e|I1KGh? M{KzN2ROW09? NGL3WoNKdmO{ZXHz[ZMh[2WubDDhdI9xfG:|aYO= M37NUlI1OzZ5Nki4
Neural progenitor cells MWXGeY5kfGmxbjDBd5NigQ>? MlHMN{DPxE1? MmrENVAh\A>? NWjwR2Z7TE2VTx?= NG\PVWNKdmS3Y3XzJIdmdmW{YYTpc44hd2ZibnX1doFtKHC{b3flcol1d3JiY3XscJM> NX[4V2t1OjR4M{iwN|Q>
ES-D3 Ml\2R5l1d3SxeHnjJGF{e2G7 NVjYVItCOTEEoN88US=> MlHON{Bl M{\4O2ROW09? NVvH[|Y3UW6mdXPld{Bud2SncnH0[UBkgXSxdH;4bYNqfHlid3n0bEBKSzVyIH;mJFQvQSEQvF2= NFHN[5YzPDd5OUO2OS=>
ES-CCE MXrDfZRwfG:6aXOgRZN{[Xl? NETrdokyOMLizszN NYXhR2l7OyCm MXfEUXNQ NF\6fFZKdmS3Y3XzJI1w\GW{YYTlJIN6fG:2b4jpZ4l1gQ>? MljxNlQ4Pzl|NkW=
ES-D3 NUTWfHlzTnWwY4Tpc44hSXO|YYm= MYW1xsDPxE1? NWXBOVZMOyCm NFfNc4lFVVOR Mk\jV5Rzd26pbImgbY5lfWOnczD0bIUh[WO2aY\peJkhd2ZiV370M4JmfGFvY3H0[Y5qdiCyYYToe4F6 MX[yOFc4QTN4NR?=
ES-CCE MXHGeY5kfGmxbjDBd5NigQ>? M3XQbFXDqM7:TR?= NXfNdXFbOyCm MYPEUXNQ M{j4TnN1em:wZ3z5JIlv\HWlZYOgeIhmKGGldHn2bZR6KG:oIGfueE9j\XSjLXPheIVvcW5icHH0bJdigQ>? MUSyOFc4QTN4NR?=
SW982 NGPtSXRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3zQfGlEPTB;MD6yNVQyPiEQvF2= MXHTRW5ITVJ?
GCT NImzNm5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlvjTWM2OD1yLkOwOlczKM7:TR?= NHnLc|VUSU6JRWK=
769-P MofrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmfZTWM2OD1yLkW4OFk6KM7:TR?= M37CTnNCVkeHUh?=
LOXIMVI M4jVOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXnJR|UxRTBwNUi4N|Ih|ryP MnrRV2FPT0WU
D-336MG NH7TNpdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnPPTWM2OD1yLki0OlIh|ryP M1L4bHNCVkeHUh?=
S-117 M1vDOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEnmR5pKSzVyPUCuPVIyOzFizszN MUfTRW5ITVJ?
D-247MG MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3e1UmlEPTB;MD65OFg2OiEQvF2= NYDLSHlQW0GQR1XS
TYK-nu M2TlcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlfETWM2OD1zLkC3PVEzKM7:TR?= NELje4dUSU6JRWK=
A498 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1H4fWlEPTB;MT6zNFQ3QSEQvF2= MVrTRW5ITVJ?
BCPAP NHT5To9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYXJR|UxRTFwNk[4PVgh|ryP NHzOS3RUSU6JRWK=
KYSE-180 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkLoTWM2OD1zLkeyO|g5KM7:TR?= M2LTR3NCVkeHUh?=
MIA-PaCa-2 MlHDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXnUepRJUUN3ME2xMlc2QTd5IN88US=> MX7TRW5ITVJ?
HCC1395 NUT6b2UyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWfJR|UxRTFwN{iyO|Mh|ryP NFfyUmhUSU6JRWK=
RS4-11 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmnVTWM2OD1zLkm1OFE2KM7:TR?= MULTRW5ITVJ?
NCI-H2122 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYjJR|UxRTJwMke2PFQh|ryP MY\TRW5ITVJ?
SNU-423 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NE[wVJBKSzVyPUKuOFk4QDdizszN M2XKeHNCVkeHUh?=
SK-LMS-1 MnntS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4nVbmlEPTB;Mj62OFEzQCEQvF2= NIO2ZlJUSU6JRWK=
RPMI-7951 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlLiTWM2OD1{LkewNVk{KM7:TR?= NYPZ[5VPW0GQR1XS
COLO-829 Ml:yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWDJR|UxRTJwN{[1OEDPxE1? MU\TRW5ITVJ?
SCC-9 NInSd4dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3rpfmlEPTB;Mj65OVQ5PSEQvF2= NXLqW21SW0GQR1XS
A704 M1fxXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2n0OmlEPTB;Mz6wPFEzKM7:TR?= NGLZ[Y5USU6JRWK=
HOP-62 NHzUSHFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NW\mXW1yUUN3ME2zMlM3ODV2IN88US=> NIXmTFNUSU6JRWK=
HCC1569 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWPBPWpVUUN3ME2zMlQ5OzB3IN88US=> MYTTRW5ITVJ?
A172 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYrJR|UxRTNwN{W0PVgh|ryP MWDTRW5ITVJ?
MOLT-16 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVjJR|UxRTNwOEi1OVYh|ryP NVO4TFM4W0GQR1XS
TE-15 M3yyN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFK1cpBKSzVyPUOuPVU{PDNizszN MlfVV2FPT0WU
OE19 NETlc4hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUXJR|UxRTNwOU[0OVEh|ryP NFPPdWpUSU6JRWK=
MHH-ES-1 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF;kTm5KSzVyPUSuNFQ2PDVizszN M4WzSXNCVkeHUh?=
NKM-1 MnTsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGDZbGtKSzVyPUSuNVM6PzFizszN NFfmO5FUSU6JRWK=
RCC10RGB MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2fpdmlEPTB;ND6yPVQ4QSEQvF2= M{nKXXNCVkeHUh?=
BxPC-3 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mkf2TWM2OD12LkewN|kyKM7:TR?= MYPTRW5ITVJ?
ALL-PO NUDHR3JYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF\kR4RKSzVyPUSuPFM5PThizszN MU\TRW5ITVJ?
SK-OV-3 Ml\nS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnvuTWM2OD12Lkm5N|Qh|ryP NWSzSY53W0GQR1XS
SW1710 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3u4[mlEPTB;NT60NlQ2PiEQvF2= MojOV2FPT0WU
NCI-H2452 NYO4cWNET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIjQRVNKSzVyPUWuOFU3OjhizszN NV:0ZYNsW0GQR1XS
D-392MG MoG3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmLzTWM2OD13LkS5NVQ2KM7:TR?= MWPTRW5ITVJ?
LB2518-MEL NEDScmZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmPXTWM2OD13LkeyOlI4KM7:TR?= NVX0OoMxW0GQR1XS
BL-41 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NW[5dm9zUUN3ME21MlgyOjZ4IN88US=> NYLmZVd7W0GQR1XS
WM-115 NVX0ZWFUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHOwSZpKSzVyPUWuPFE2ODlizszN M33jRXNCVkeHUh?=
SK-MEL-2 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYnuO5lzUUN3ME22MlM5OzR2IN88US=> NFrmRpdUSU6JRWK=
CGTH-W-1 MkHqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3XHZmlEPTB;Nj60NVch|ryP MUnTRW5ITVJ?
BB65-RCC MlXoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGDwRpJKSzVyPU[uOVMyQTVizszN NFr0OopUSU6JRWK=
CAL-85-1 MkTzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4rEfWlEPTB;Nj62NFk{PiEQvF2= M4L5XnNCVkeHUh?=
RT-112 M1m2N2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWTXenhZUUN3ME23MlE3PDV4IN88US=> NFezR4xUSU6JRWK=
MC116 NFrT[ZJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NW\xVHd3UUN3ME23MlI5PjlzIN88US=> NIPKO2VUSU6JRWK=
G-361 NYDyVG5xT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn74TWM2OD15LkS2OVYyKM7:TR?= Mnr3V2FPT0WU
SW1417 M1HLUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX\JR|UxRTdwNkS1PFQh|ryP MnvKV2FPT0WU
MV-4-11 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXfJR|UxRThwNUSzNFQh|ryP M3H2c3NCVkeHUh?=
CAL-72 MkDhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2ntUmlEPTB;OD63OlEyOyEQvF2= NI\veotUSU6JRWK=
MKN28 M2HOUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MljpTWM2OD16Lki4OVE3KM7:TR?= Mk\qV2FPT0WU
MEG-01 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWDJR|UxRThwOUmwOFMh|ryP M37rXHNCVkeHUh?=
ACHN NEPic2ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mm\UTWM2OD17LkOwOVQ3KM7:TR?= Mni2V2FPT0WU
NCI-H1755 NIL3Z4tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUDJR|UxRTlwM{m5PFYh|ryP MYLTRW5ITVJ?
NCI-H64 M2fWeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFTNPFNKSzVyPUmuOFIzPTVizszN MULTRW5ITVJ?
COLO-205 M2[3ZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoTxTWM2OD17LkSzPFIyKM7:TR?= NYTzW4VTW0GQR1XS
CTV-1 M1PXc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWrlb3RqUUN3ME25MlQ{QDl2IN88US=> M3vQO3NCVkeHUh?=
U-2-OS M3\pXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NW\HO5lLUUN3ME25MlQ2ODB4IN88US=> MY\TRW5ITVJ?
LB996-RCC NIPa[IhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn7HTWM2OD17LkWyN|k4KM7:TR?= MXHTRW5ITVJ?
SK-N-DZ NX23WnN3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlPKTWM2OD17Lk[1O|QzKM7:TR?= NVLWVWZmW0GQR1XS
EFM-19 M4jlUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mln5TWM2OD17Lke1OVY3KM7:TR?= M3O5XXNCVkeHUh?=
DBTRG-05MG MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mk\oTWM2OD17Lke3O|ch|ryP M1;KfnNCVkeHUh?=
U-118-MG NXfiNZd2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGrYVINKSzVyPUmuPVQ1PDRizszN NYXYSZR3W0GQR1XS
LB1047-RCC M13J[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4TRTmlEPTB;OT65PVc6QCEQvF2= NELmbWNUSU6JRWK=
NCI-H28 NF;4UZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIn5Z21KSzVyPUGwMlQ1OTNizszN MYHTRW5ITVJ?
MZ2-MEL MlTZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGHYXXpKSzVyPUGwMlQ3PzdizszN MkHwV2FPT0WU
M059J M3PwZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF;1VnZKSzVyPUGwMlYyODJizszN MY\TRW5ITVJ?
HT-3 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml3ITWM2OD1zMD62NVc6KM7:TR?= M3[3bnNCVkeHUh?=
SNU-387 NIPubVFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXrJR|UxRTFyLkeyN|Uh|ryP M2nPXXNCVkeHUh?=
NCI-H526 NHHXT2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV3JR|UxRTFzLkGzOlUh|ryP NEjjdWtUSU6JRWK=
HEL NI\lbWtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUTJR|UxRTFzLk[1O|kh|ryP M1XYTHNCVkeHUh?=
PA-1 NIXzPGNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXXLUGdFUUN3ME2xNU46OyEQvF2= MoDhV2FPT0WU
PC-14 NHPIdVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUOybZk2UUN3ME2xNk4{OjRizszN M2D3R3NCVkeHUh?=
C8166 NInkdpFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUnRWllYUUN3ME2xN{4zOzF6IN88US=> MkCxV2FPT0WU
NCI-H82 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXjJR|UxRTF|LkK5NFkh|ryP MmXmV2FPT0WU
DSH1 M2nudmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWfJR|UxRTF|LkO4O|kh|ryP M1jHb3NCVkeHUh?=
T98G MmXLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVLVdXpXUUN3ME2xN{41PzF5IN88US=> NH7V[JhUSU6JRWK=
KU812 M1PNUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml\VTWM2OD1zMz61NVc2KM7:TR?= MlrVV2FPT0WU
SW48 Mn3uS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmTMTWM2OD1zMz63NlY2KM7:TR?= M{Lkd3NCVkeHUh?=
MEL-HO M3;1Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3XYfGlEPTB;MUOuPFgzQSEQvF2= M4Pid3NCVkeHUh?=
SF539 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn;DTWM2OD1zND6wNVE3KM7:TR?= MY\TRW5ITVJ?
NB1 NFPqOIFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXTJR|UxRTF2LkG5N|Uh|ryP NWHSN|lqW0GQR1XS
ST486 NV3Qcod{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWnJR|UxRTF2LkKyOVEh|ryP MULTRW5ITVJ?
LoVo NWDsS5JET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYTMcmNRUUN3ME2xOU4zPjV7IN88US=> NVTsfFRbW0GQR1XS
NH-12 NHTMW5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEnWS4pKSzVyPUG2MlAxQTdizszN NV;0WYl4W0GQR1XS
CCRF-CEM NWfWSoZuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVTGV3M3UUN3ME2xOk43PzB{IN88US=> MlezV2FPT0WU
Daudi MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4\IcGlEPTB;MUeuOlE1QSEQvF2= MUXTRW5ITVJ?
SIMA M3LiXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVHZUWRoUUN3ME2xO{46OTF6IN88US=> Mn35V2FPT0WU
8305C MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHLpXZJKSzVyPUG4MlU{ODVizszN MWrTRW5ITVJ?
EM-2 NV3T[Wg6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MljhTWM2OD1zOD62NlQ5KM7:TR?= M2jXNHNCVkeHUh?=
SNU-449 M1nHRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkjETWM2OD1zOD63NVUh|ryP NWfweFBLW0GQR1XS
A101D M1;E[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlXUTWM2OD1zOT6yOFMh|ryP NEXibIpUSU6JRWK=
MHH-NB-11 M4L2[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mm\6TWM2OD1zOT61N|QzKM7:TR?= M3PJN3NCVkeHUh?=
Becker MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MY\JR|UxRTJyLkG3O|ch|ryP NIK3RWNUSU6JRWK=
NCI-H727 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml\MTWM2OD1{MD6yNVY6KM7:TR?= NHm2TplUSU6JRWK=
HCC1187 NVP0VHdQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1jaWGlEPTB;MkCuN|U5QSEQvF2= Mn22V2FPT0WU
KYSE-450 MoDTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFvYdIdKSzVyPUKxMlU{PTRizszN NHvycmtUSU6JRWK=
SK-MEL-28 NH3lXJVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUDJR|UxRTJzLk[4OlMh|ryP NHvLe4ZUSU6JRWK=
BPH-1 NX;rS3hDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3\BWWlEPTB;MkGuO|A{PSEQvF2= NX\ZZWVtW0GQR1XS
EoL-1- NYfyOFNGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MonpTWM2OD1{MT64N|g2KM7:TR?= MXnTRW5ITVJ?
TGBC24TKB M4PDXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEHlOVZKSzVyPUKxMlg6PTdizszN NUK0dpZsW0GQR1XS
KARPAS-299 MkTCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2niXWlEPTB;MkKuNVk2PCEQvF2= M1G2T3NCVkeHUh?=
SW684 M37n[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGjnTW9KSzVyPUKyMlI5PThizszN NUD3empXW0GQR1XS
RXF393 NGDrPFFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIjqSlVKSzVyPUKyMlYxPjhizszN NV:1PYlYW0GQR1XS
LS-513 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmXDTWM2OD1{Mj62NVkzKM7:TR?= M1\WPXNCVkeHUh?=
KURAMOCHI MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV;BUoNxUUN3ME2yNk44ODZ7IN88US=> NV\KPWxEW0GQR1XS
GI-1 M1PhWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYS2XohlUUN3ME2yNk44QDRzIN88US=> NFjOSZpUSU6JRWK=
M14 NXuxV3VYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWr6ZoI{UUN3ME2yN{4yOTN{IN88US=> NWLUeItuW0GQR1XS
REH MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoHhTWM2OD1{Mz6xOlk6KM7:TR?= MUDTRW5ITVJ?
K5 NH\pcohIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYW3UGw1UUN3ME2yN{42PDh4IN88US=> MlrqV2FPT0WU
PANC-10-05 M3LlO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVnJR|UxRTJ2LkC4PVgh|ryP MX7TRW5ITVJ?
HMV-II NHfwOWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2HVUmlEPTB;MkSuNlE3OyEQvF2= M1zvb3NCVkeHUh?=
NCI-H1963 NGeyWYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2G1fGlEPTB;MkSuNlg{QSEQvF2= NGLQdIpUSU6JRWK=
LU-139 M4DzWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4f0OWlEPTB;MkSuPFM2KM7:TR?= NXfObFlvW0GQR1XS
KASUMI-1 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoLaTWM2OD1{ND65N|I5KM7:TR?= M2WwZ3NCVkeHUh?=
RO82-W-1 NXWyWJlqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{jYeWlEPTB;Mk[uO|M4PyEQvF2= MYfTRW5ITVJ?
NCI-H1770 NEGwb4xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2Ds[mlEPTB;Mk[uPFk5PiEQvF2= NF;KVYdUSU6JRWK=
SNG-M MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX3IfnJPUUN3ME2yO{4zOjdzIN88US=> NGXaV4lUSU6JRWK=
KLE NYjFNXJDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUC1[FlKUUN3ME2yO{4zPTZ3IN88US=> M3rGXXNCVkeHUh?=
NCI-H2170 NYTzWmxvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NULVbWQzUUN3ME2yO{42Ojh{IN88US=> NIXjUJVUSU6JRWK=
COLO-792 NEfRdZVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYDJR|UxRTJ5Lke3Nlgh|ryP NWm4c5ppW0GQR1XS
ES8 NVW1SY86T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NG\2b5pKSzVyPUK3Mlc5OTRizszN NXm4Sm0yW0GQR1XS
MC-CAR NVzOOHpET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mnz5TWM2OD1{Nz63PVA4KM7:TR?= NWLkW4tXW0GQR1XS
SU-DHL-1 NGfkZ|ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVLJR|UxRTJ5Lkm0O|Qh|ryP NYL2XZB7W0GQR1XS
OAW-28 NXzMN|VST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVXNN4h{UUN3ME2yPE4xODV{IN88US=> M3;CZnNCVkeHUh?=
C32 NGfYNVdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2PQWmlEPTB;MkiuNFgzPiEQvF2= NX70cmhQW0GQR1XS
SW872 NV;zfJVvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1\VdmlEPTB;MkiuOVMzPiEQvF2= M3vienNCVkeHUh?=
JiyoyeP-2003 NGHwSFlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVjJR|UxRTJ7Lk[3NVkh|ryP MmOwV2FPT0WU
A204 NFHBOJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFzNZndKSzVyPUK5MlY5OjhizszN NYTPV4FNW0GQR1XS
IST-MES1 Mmn2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFnUfppKSzVyPUK5MlcxQTJizszN MUjTRW5ITVJ?
LCLC-103H MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MY\JR|UxRTNyLkKwOVkh|ryP M1Hve3NCVkeHUh?=
CAKI-1 M{OxXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1rlZ2lEPTB;M{CuNlY2PiEQvF2= MYDTRW5ITVJ?
NALM-6 NV7lTnpwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWDiZYNCUUN3ME2zNE41QTZ3IN88US=> MVvTRW5ITVJ?
SK-MM-2 M4XWU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn7iTWM2OD1|MD65O|kzKM7:TR?= M1rRZ3NCVkeHUh?=
LB373-MEL-D MoHUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYfJR|UxRTNzLkCwOFch|ryP Ml\XV2FPT0WU
CAS-1 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnvtTWM2OD1|MT63OlY2KM7:TR?= M4rDRXNCVkeHUh?=
KNS-42 NFHBT4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIX1eIhKSzVyPUOxMlg3PjJizszN MljJV2FPT0WU
SW948 MnfKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV3JR|UxRTN{Lke1OFgh|ryP MoTXV2FPT0WU
HCC1143 MkPNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2PofWlEPTB;M{KuO|k4QSEQvF2= M3\icXNCVkeHUh?=
NCI-H1648 MormS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4nRRWlEPTB;M{KuPFQ3PyEQvF2= NF:1OW1USU6JRWK=
RVH-421 M4jH[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXHTR|BpUUN3ME2zN{4yPjhizszN NHLISphUSU6JRWK=
KU-19-19 M4ixV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnHxTWM2OD1|Mz63OVM1KM7:TR?= MULTRW5ITVJ?
PFSK-1 NYHzW2FNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFnYWY1KSzVyPUO0MlkzPDFizszN MoniV2FPT0WU
TK10 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3mwNGlEPTB;M{WuNVMzOiEQvF2= M{DF[nNCVkeHUh?=
IGROV-1 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1nrVGlEPTB;M{WuN|QyPiEQvF2= M3njRXNCVkeHUh?=
EFE-184 M3TRbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXvyTmh3UUN3ME2zOU46OTdizszN NUDWbpQ{W0GQR1XS
8505C MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGLlOWZKSzVyPUO1MlkzQTRizszN NVHpOHhSW0GQR1XS
SK-UT-1 M1uwSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXXJR|UxRTN4LkOzN|Eh|ryP NGrTR|FUSU6JRWK=
MKN1 Moq4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGnpUphKSzVyPUO2Mlg1PDRizszN NInhUGJUSU6JRWK=
no-10 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4TrUmlEPTB;M{iuNVI6PCEQvF2= M122NnNCVkeHUh?=
HT-144 Ml\xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkjvTWM2OD1|OD6yOVc2KM7:TR?= MlHEV2FPT0WU
CMK NELy[4FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV;JR|UxRTN6LkSxOlIh|ryP M{jkenNCVkeHUh?=
KM12 M4PESGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlXrTWM2OD1|OD61PVk5KM7:TR?= NU\SSHNkW0GQR1XS
HCC2218 MmPtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX;JR|UxRTRyLkW4OFkh|ryP Mmq3V2FPT0WU
BE-13 MmLES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml;JTWM2OD12MT60OFc{KM7:TR?= MXjTRW5ITVJ?
SK-CO-1 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4m1VWlEPTB;NEGuO|c2PSEQvF2= M3LtVXNCVkeHUh?=
MPP-89 MoTvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1PLUWlEPTB;NEGuPVIyOSEQvF2= M{D3c3NCVkeHUh?=
NY NV3KTlBHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4qzemlEPTB;NEKuNVgxOSEQvF2= MnfrV2FPT0WU
IST-MEL1 MlG5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWjVVYxZUUN3ME20Nk43QTN6IN88US=> MnTOV2FPT0WU
HCT-116 M3f3Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX3Jbm5MUUN3ME20N{4zODl7IN88US=> Mn[3V2FPT0WU
LB831-BLC NIL6O3JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX7LOW1qUUN3ME20N{43QTdizszN NHfSS5FUSU6JRWK=
TE-8 M3rQVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlvHTWM2OD12Mz63PFk2KM7:TR?= NWfhS|Y5W0GQR1XS
SR M2PDTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmnpTWM2OD12Mz64Olc{KM7:TR?= MnywV2FPT0WU
ESS-1 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYTJR|UxRTR2LkKwOlIh|ryP NFfKR3FUSU6JRWK=
KYSE-270 NGLifVZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3\KOGlEPTB;NEWuNFQ{PyEQvF2= MlzmV2FPT0WU
H4 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYnPPGc2UUN3ME20OU4{Ojl4IN88US=> NUL3d5pKW0GQR1XS
COLO-800 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkXMTWM2OD12NT61PFk5KM7:TR?= MXTTRW5ITVJ?
Ramos-2G6-4C10 NGnlZoJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWXJR|UxRTR4LkC3OVUh|ryP M1;3dnNCVkeHUh?=
no-11 NFi4T5BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmDhTWM2OD12Nj6yNFMyKM7:TR?= NWjodmJbW0GQR1XS
UACC-62 NY\Fd3FxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV7RO3FtUUN3ME20Ok45PjB6IN88US=> MYnTRW5ITVJ?
D-542MG NXu2bJdbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVPjWFNlUUN3ME20O{41PTh7IN88US=> M1Py[HNCVkeHUh?=
NCI-H747 NF\E[pVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NELVV2xKSzVyPUS3MlczPjRizszN NEDkWGlUSU6JRWK=
HCC2998 NHnCbYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWPJR|UxRTR6LkK5PFIh|ryP MWTTRW5ITVJ?
SNB75 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2nhV2lEPTB;NEiuOFEyQCEQvF2= M3jmUnNCVkeHUh?=
COR-L105 M2f4Rmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX;JR|UxRTR6Lkm3N{DPxE1? NFf5[oZUSU6JRWK=
SNU-C1 Moj4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV3JR|UxRTR7LkOyNFch|ryP NUHkNYdmW0GQR1XS
LC4-1 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEPVXIVKSzVyPUS5Mlg6OTJizszN M1\zVHNCVkeHUh?=
KS-1 NH3vRZVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXHJR|UxRTVyLkKyNlUh|ryP MlPXV2FPT0WU
NCI-H1793 NXOxR3d5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGWzNodKSzVyPUWxMlI{OSEQvF2= MmHhV2FPT0WU
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DB NFHxUphIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2H4d2lEPTB;NUGuPVg2PSEQvF2= M376UnNCVkeHUh?=
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DV-90 MmDIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml7mTWM2OD13NT64PVE4KM7:TR?= NX30RmhGW0GQR1XS
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HT-1376 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlqzTWM2OD13OT6wPVIyKM7:TR?= Mkm1V2FPT0WU
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NCI-H1703 NHu1V25Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX;kZZczUUN3ME22PE4yOTdzIN88US=> MYPTRW5ITVJ?
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NCI-H292 NHrGSXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX3wbZZ1UUN3ME25PU4{ODB2IN88US=> NYD3VVFoW0GQR1XS
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DU-145 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXLJR|UxRTFyOT65OFUh|ryP MVPTRW5ITVJ?
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HAL-01 M4G0Wmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWfJR|UxRTF{Mj60PVkh|ryP M1npOnNCVkeHUh?=
HT-29 M2rvZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVHJR|UxRTF{Mj63O|gh|ryP MnT0V2FPT0WU
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SW1463 MnHzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV7JR|UxRTF{ND6wO|ch|ryP MljTV2FPT0WU
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... Click to View More Cell Line Experimental Data

In vivo Oral administration of CHIR-99021 at 30 mg/kg enhances glucose metabolism in a rodent model of type 2 diabetes, with a maximal plasma glucose reduction of nearly 150 mg/dl 3-4 hours after administration, while plasma insulin remains at or below control levels. Oral administration of CHIR-99021 at 16 or 48 mg/kg 1 hour before oral glucose challenges in ZDF rats significantly improves glucose tolerance with 14% and 33% reduction in plasma glucose at 16 mg/kg and 48 mg/kg, respectively, and the higher dose of CHIR-99021 also reduces hyperglycemia before the oral glucose challenge. [1] Administration of CHIR-99021 significantly augments hematopoietic repopulation in recipient mice transplanted with mouse or human hematopoietic stem cells (HSCs), suggesting that GSK-3 is a specific modulator of HSC activity. [4]

Protocol

Kinase Assay:

[1]

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Cell-free kinase assays:

Polypropylene 96-well plates are filled with 300 μL/well buffer (50 mM tris HCl, 10 mM MgCl2, 1 mM EGTA, 1 mM dithiothreitol, 25 mM β-glycerophosphate, 1 mM NaF, 0.01% BSA, pH 7.5) containing 27 nM GSK-3α or 29 nM GSK-3β, and 0.5 μM biotin-CREB peptide substrate. Various concentrations of CHIR-99021 are added in 3.5 μL of DMSO, followed by 50 μL of ATP stock to yield a final concentration of 1 μM ATP in all cell-free assays. After incubation, triplicate 100-μL aliquots are transferred to Combiplate eight plates containing 100 μL/well 50 μM ATP and 20 mM EDTA. After 1 hour, the wells are rinsed five times with PBS, filled with 200 μL of scintillation fluid, sealed, left 30 minutes, and counted in a scintillation counter. All steps are performed at room temperature.
Cell Research:

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  • Cell lines: INS-1E
  • Concentrations: Dissolved in DMSO, final concentrations ~ 20 μM
  • Incubation Time: 1, or 4 days
  • Method:

    Cells are maintained for 24 hours in starvation medium (culture medium with only 5 mM glucose, 1% fetal calf serum). Then cells are exposed to various concentrations of CHIR-99021 for 1, or 4 days. Cell number is measured by staining of cellular DNA with CyQuant dye, which becomes fluorescent when bound to DNA. Fluorescence is measured after 30 minutes of incubation using the FLUOstar Optima reader. Cell replication is determined by BrdUrd incorporation. BrdUrd labeling solution is added to the medium for the last 4 hours before the cells are fixed using FixDenat solution and incubated with monoclonal anti-BrdUrd-POD antibodies. After substrate solution is added to each well, the light emission is measured in a microplate luminometer using the Analyst HT detection system.


    (Only for Reference)
Animal Research:

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  • Animal Models: Female db/db mice or male ZDF rats with type 2 diabetes
  • Formulation: Formulated as solutions in 20 mM citrate-buffered 15% Captisol or as fine suspensions in 0.5% carboxymethylcellulose.
  • Dosages: ~48 mg/kg
  • Administration: Orally
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 93 mg/mL (185.33 mM)
Ethanol 2 mg/mL (3.98 mM)
Water Insoluble
In vivo Add solvents individually and in order:
5% DMSO+30% PEG 300+ddH2O
24mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 501.8
Formula

C22H18Cl2N8.HCl

CAS No. 252917-06-9 (free base)
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    I recently purchased CHIR 99021 to inject mice intraperitoneally. The data sheet says using 10% captisol for in vivo use. Is it for IP injection? Or do you have other solutions to suggest to use for in vivo IP injection of CHIR 99021?

  • Answer:

    The formulation of 15% Captisol is for oral gavage, and S2924 CHIR-99021 (CT99021) HCl in it is a suspension. S2924 CHIR-99021 (CT99021) HCl can be dissolved in 5% DMSO+30% PEG 300+ddH2O at 24mg/ml as a clear solution for IP injection.

  • Question 2:

    How about the half-life of this compound? If it is necessary for daily treatment when the compound is used in long-term experiment?

  • Answer:

    Please change the culture medium each day to add the new comound in it if the duration is more than 72h.

  • Question 3:

    What is the difference between S2924 and S1263? Which one is better for differentiation?

  • Answer:

    S1263 is the free base format of Chir-99021, while S2924 is the HCl salt format. They have the same biological activity but are slightly different in solubility.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID