CHIR-99021 (CT99021) HCl

Catalog No.S2924

CHIR-99021 (CT99021) HCl Chemical Structure

Molecular Weight(MW): 501.8

CHIR-99021 HCl (CT99021) is hydrochloride of CHIR-99021, which is a GSK-3α/β inhibitor with IC50 of 10 nM/6.7 nM; CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs Cdc2 and ERK2.

Size Price Stock Quantity  
In DMSO USD 340 In stock
USD 90 In stock
USD 170 In stock
USD 570 In stock
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Cited by 70 Publications

9 Customer Reviews

  • Differentiation induced by treatment with the Gsk3 inhibitor in hPSCs is β-catenin dependent. H9-7TGP cells were treated with 12 uM CH in mTeSR1 for 4 d. Immunofluorescent staining for Oct4, Isl1, and Nkx2.5 was compared with GFP expression. (Scale bars, 50 uM.)

    Proc Natl Acad Sci U S A 2012 109(27):E1848-57. CHIR-99021 (CT99021) HCl purchased from Selleck.

    Gpc4 regulates ESC response to Wnt/β-catenin signaling.  (E) Quantitative analysis of control and Gpc4-mutant beating foci exposed to the GSK3 inhibitor, CHIR 99021 (1 and 3 μM, from day 0 to day 5). Analysis was done at different time points. Gpc4- mutant cells treated with CHIR 99021 behave as controls (mean±SEM; n=2).

    Stem Cells 2012 30, 1863-1874. CHIR-99021 (CT99021) HCl purchased from Selleck.

  • Inhibition of GSK3 activity induces an autophagic response in human pancreatic cancer cells. PANC1 cells were treated for 24 h with DMSO, CHIR99021 (CHIR; 5 uM) or the mTOR inhibitor Torin1 (250 nM). Autophagosome (punctate LC3B) (D) and lysosome (E) labeling was visualized with a Zeiss LSM700 confocal microscope. Images were acquired with a 20x (D) or 63x (E) objective. Nuclei were stained with DAPI. Scale bars, 20 祄.

    J Biol Chem 2015 290(9), 5592-605 . CHIR-99021 (CT99021) HCl purchased from Selleck.

     

    Characterization of rES cells. A: image of normal rES cell colonies on feeder layers. B: rES colonies were positive for AP staining. CeE: rES colonies readily expressed pluripotent markers, Sox2 (C), Oct4 (D), and SSEA-1 (E). Blue, DAPI. Scale bars: 100 um.

    J Genet Genomics 2012 39, 643e651. CHIR-99021 (CT99021) HCl purchased from Selleck.

  •  

    Smad linker phosphorylation involves GSK3 in human melanoma lines. Inhibition of Smad2 and Smad3 linker phosphorylation in the presence of the specific GSK3 specific inhibitor, CT99021. 24 hours post seeding, WM793 and 1205LU cells were serum-starved for about 16 hours, and incubated in the absence (2) or presence (+) of 2 uM of CT99021 for two hours. Immunoblots were performed as in A. p: Phosphorylated. S: Serine; T: Threonine.

    PLoS One 2012 7, e47312. CHIR-99021 (CT99021) HCl purchased from Selleck.

    A. Vero cells were pretreated with the indicated concentrations of CHIR-99021 for 1 h and then infected with PEDV (MOI = 1) for 8 or 10 h. Cell lysates were prepared and analyzed using Western blotting.

    PLoS One, 2016, 11(8):e0161508. CHIR-99021 (CT99021) HCl purchased from Selleck.

  • SKOV3/DDP cells were treated with a range of concentration of (A) CHIR-99021 or in combination with 20 µM cisplatin for 48 h.

    Exp Ther Med, 2016, 12(6):3851-3858. CHIR-99021 (CT99021) HCl purchased from Selleck.

     

    PANC1 cells were treated with GSK3 inhibitors SB216763 (SB 10µM or SB 20µM) or CHIR99021 (CHIR 5µM) for 72h.

    CHIR-99021 (CT99021) HCl purchased from Selleck.

  •  

     Activity of canonical WNT/ β -CATENIN signals induced by CHIR 99021 in the developing human lung. Human lung tissue explants obtained at 15 weeks of gestation were exposed to 0, 5 and 10 μM CHIR 99021 in vitro for 72 h. (A-B) Western blot analysis showed expression of β -CATENIN in human lung tissues. Results are presented as the mean ± sem of three independent experiments. *P < 0.05, **P < 0.01. (C) qRT-PCR analysis detected a dose-dependent increase in WNT signaling transcription factors and target genes following exposure to CHIR 99021. Results are presented as the mean ± sem of three independent experiments. *P < 0.05, **P < 0.01. (D-K) Histology examination showed obvious morphogenetic changes of the airway epithelial cells associated with CHIR 99021 treated lung tubes. Scale bars: D-G =100 μm; H-K = 20 μm.

    CHIR-99021 (CT99021) HCl purchased from Selleck.

Purity & Quality Control

Choose Selective GSK-3 Inhibitors

Biological Activity

Description CHIR-99021 HCl (CT99021) is hydrochloride of CHIR-99021, which is a GSK-3α/β inhibitor with IC50 of 10 nM/6.7 nM; CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs Cdc2 and ERK2.
Targets
GSK-3β [1]
(Cell-free assay)
GSK-3α [1]
(Cell-free assay)
6.7 nM 10 nM
In vitro

CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs CDC2 and ERK2, as well as other protein kinases. Furthermore, CHIR-99021 shows only weak binding to a panel of 22 pharmacologically relevant receptors and little inhibitory activity against a panel of 23 nonkinase enzymes. CHIR-99021 induces the activation of glycogen synthase (GS) in insulin receptor-expressing CHO-IR cells with EC50 of 0.763 μM. [1] In addition to simulating the actions of insulin, inhibition of GSK-3 by CHIR-99021 (3 μM) increases free cytosolic β-catenin by 1.9-fold, mimicking the canonical Wnt signaling pathway in 3T3-L1 preadipocytes. During any of the first 3 days of differentiation, CHIR-99021 treatment inhibits the preadipocyte differentiation with IC50 of 0.3 μM by blocking induction of CCAAT/enhancer-binding protein α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ). [2] Unlike lithium chloride and AR-A014418, CHIR-99021 treatment does not reduce the viability of INS-1E cells even at high concentrations. Instead, CHIR-99021 robustly increases the rate of proliferation of INS-1E cells in a dose-dependent manner, and significantly inhibits INS-E cell death induced by high glucose and high palmitate in a concentration-dependent manner. CHIR-99021 promotes primary beta cell replication in isolated rat islets at concentrations as low as 1 μM, with 2-3 fold increase of cell replication at 5 μM of CHIR-99021 treatment. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
3T3-L1 NU\LbVNVTnWwY4Tpc44hSXO|YYm= NFHBcpM{KM7:TR?= M{fqbVQhcA>? MmW2SG1UVw>? M2LwVWlvcGmkaYTzJJBz\WGmaYDvZ5l1\SCmaX\m[ZJmdnSrYYTpc44> NUi4XYlQOTJyNUWyNFA>
insulin receptor–expressing CHO-IR M2CwRWZ2dmO2aX;uJGF{e2G7 M{XwO|UxOCCwTR?= NITpRYY{OCCvaX6= MVTEUXNQ NEfoWHhC[3SrdnH0[ZMhT2y7Y3;n[Y4hW3mwdHjhd4UhMEeVKR?= MmK4NVI3ODZ2OUe=
H4IIE M4rrXGZ2dmO2aX;uJGF{e2G7 MkLrNVAh|ryP MnfvNlQhcA>? NY\kO5RoTE2VTx?= NFrmSWVT\We3bHH0[ZMh|rJvY3H0[Y5qdiCjY4Tpeol1gSCjbnSgWGlTTSClb370ZYlvcW6pIIDyc41wfGW{IHHjeIl3cXS7 MnPVNVU{PTBzOUW=
Oct4/Sox2/Klf4-infected MEFs MmLPSpVv[3Srb36gRZN{[Xl? NFLibIIyOCEQvF2= MUGyPEBl NFXQU2JFVVOR MVvQdo9ud3SnczDNSWZ{KHKncILv[5JidW2rbnegWJJidnOmdXPl[EBjgSCRY4S0MEBUd3h{LDDhcoQhU2yoNB?= MVqxPVg{QTB3NR?=
Sca-1+ HPC Mnq4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGroZWIzKM7:TR?= M{TifVYh\A>? MlPmSG1UVw>? MnfFV5lv\XKpaYP0bYNidGy7IIDyc41wfGW|IGPjZU0yM8LiSGDDJJBzd2yrZnXyZZRqd25iYX7kJINwdG:weTDmc5Ju[XSrb36geI9o\XSqZYKge4l1cCCHR1[= NGnTZpMzOTZ{NEO0PC=>
HMC1.2 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUOzNEDPxE1? MXq3NkBp M36wUmROW09? M{XZPHJm\HWlZYOgZ4VtdCC|dYL2bZZidA>? NYnoeVRvOjJyM{mzNFE>
Endothelial cell(EC) M3zZXmZ2dmO2aX;uJGF{e2G7 M2\oWlMh|ryP MoTMSG1UVw>? MVLQdo9ud3SnczDlcoRwfGinbHnhcEBk\WyuIDjFR{kh\GmoZnXy[Y51cWG2aX;u NFfafHgzOjB3MkCwOi=>
PANC1 MXvBdI9xfG:|aYOgRZN{[Xl? NFHFW4nDqDVizszN MoDqO|IhcA>? MULEUXNQ MoTDWJJq\2encoOgZY4h[XCxcITveIlkKHKnc4DvcpNm NECyflQzOjJyMUG4Oi=>
INS-1E NIjvWVVHfW6ldHnvckBCe3OjeR?= M1XzblIvPSEQvF2= NVv0PGl{OjRiaB?= MnTCSG1UVw>? NGP6[W5KdmS3Y3XzJIEhemWmdXP0bY9vKG:oIF7QNUBwfmW{ZYjwdoV{e2mxbh?= MnHONlI1Ojd5MES=
hiPSC-NPs MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWez5qCK|ryP NFTWdG84OiCq MmK3SG1UVw>? NGH0RoVKdmO{ZXHz[ZMh[2WubDDwdo9tcW[ncnH0bY9vyqB? NFPMOY8zOjZ2Mk[4Oy=>
platelets NW\PNJhWTnWwY4Tpc44hSXO|YYm= NIHyNnkyOCEQvF2= NFX3VZMyPSCvaX6= MULEUXNQ MlvvTY5kemWjc3XzJJRpem:vYnnuMY1m\GmjdHXkJJBt[XSnbHX0JIFo\3KnZ3H0bY9vKGGwZDDBWHAhe2WlcnX0bY9v MWCyN|I{QTh5Nx?=
DN3 MXPGeY5kfGmxbjDBd5NigQ>? MVKxNEDPxE1? M{HWOFk3KGh? NF;w[odFVVOR MYXJcoNz\WG|ZYOgZ4VtdCCmZY\lcI9xdWWwdDDhcoQheHKxbHnm[ZJifGmxbh?= NGXyd2ozOzV{Nkm4PS=>
embryonic stem cells (ESC) M{nMbGZ2dmO2aX;uJGF{e2G7 MUezJOK2VQ>? NIrLcHoyOSCm M1X4emROW09? M2HseXBzd22xdHXzJJNmdGZvcnXu[ZdidCCxZjDuZeOwfmVvdInw[UBud3W|ZTDlcYJzgW:waXOgd5RmdSClZXzsd{ApTVOFKR?= MmO2NlM5PDN|MUK=
H460 NX;XcFhtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVy2SYlkPSEEtV2= MmizNlQhcA>? M3zEZmROW09? NHmxOndRem:vb4Tld{Bk\WyuIIDyc4xq\mW{YYTpc44> NX;rXnpSOjR|Nke2PFg>
H157 NXixTmo3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYSzS3lkPSEEtV2= MmLzNlQhcA>? MVzEUXNQ Mn7tVJJwdW:2ZYOgZ4VtdCCycn;sbYZmemG2aX;u NVO3TIo2OjR|Nke2PFg>
H460 NV7PSZNQSXCxcITvd4l{KEG|c3H5 Mn\sOUDDvU1? NIj2[lkzPCCq M2K5TmROW09? M2O5[mF1fGWwdXH0[ZMh[2WubDDhdI9xfG:|aYO= MkLGNlQ{Pjd4OEi=
H157 MUnBdI9xfG:|aYOgRZN{[Xl? NWLWe5RDPSEEtV2= M3T3d|I1KGh? MXfEUXNQ NWXmdlJjTG:nczDuc5Qhe2mpbnnmbYNidnSueTDh[oZm[3RiYYDvdJRwe2m| NYj1[4ZQOjR|Nke2PFg>
A549 MkLIRZBweHSxc3nzJGF{e2G7 NIHTeZE2KML3TR?= NF\tTZEzPCCq M2ixTmROW09? M1\1RWlv[3KnYYPld{Bk\WyuIHHwc5B1d3Orcx?= M3Hi[VI1OzZ5Nki4
Neural progenitor cells NVOw[lBOTnWwY4Tpc44hSXO|YYm= M2O0O|Mh|ryP NGqwbJMyOCCm MlW0SG1UVw>? MYPJcoR2[2W|IHflcoVz[XSrb36gc4YhdmW3cnHsJJBzd2enbnn0c5Ih[2WubIO= MVGyOFY{QDB|NB?=
ES-D3 M{HCPWN6fG:2b4jpZ{BCe3OjeR?= M3LtNFExyqEQvF2= M2S2b|Mh\A>? M{mzb2ROW09? NX6zNpFHUW6mdXPld{Bud2SncnH0[UBkgXSxdH;4bYNqfHlid3n0bEBKSzVyIH;mJFQvQSEQvF2= Mo\lNlQ4Pzl|NkW=
ES-CCE NYDJPIc3S3m2b4TvfIlkKEG|c3H5 NYLwNItoOTEEoN88US=> MlrqN{Bl NUH1RXBRTE2VTx?= Mke2TY5lfWOnczDtc4RmemG2ZTDjfZRwfG:6aXPpeJk> NFXyT3czPDd5OUO2OS=>
ES-D3 M{TCemZ2dmO2aX;uJGF{e2G7 M2K1fVXDqM7:TR?= MkXvN{Bl NY\ZZmk5TE2VTx?= M{XpfnN1em:wZ3z5JIlv\HWlZYOgeIhmKGGldHn2bZR6KG:oIGfueE9j\XSjLXPheIVvcW5icHH0bJdigQ>? MljrNlQ4Pzl|NkW=
ES-CCE NVTSdmViTnWwY4Tpc44hSXO|YYm= NEPjRoQ2yqEQvF2= NFnIRmg{KGR? M175RWROW09? NGPPfVFUfHKxbnfsfUBqdmS3Y3XzJJRp\SCjY4Tpeol1gSCxZjDXcpQw[mW2YT3jZZRmdmmwIIDheIh4[Xl? M3LseVI1Pzd7M{[1
SW982 NVXETXFST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn:0TWM2OD1yLkKxOFE3KM7:TR?= NHj2[I9USU6JRWK=
GCT M4TlWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVzJR|UxRTBwM{C2O|Ih|ryP NUjPOoVQW0GQR1XS
769-P Mn21S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVTJR|UxRTBwNUi0PVkh|ryP MljJV2FPT0WU
LOXIMVI MkfsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mnj5TWM2OD1yLkW4PFMzKM7:TR?= MlH3V2FPT0WU
D-336MG NVzVOHNxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NW\PSGdXUUN3ME2wMlg1PjJizszN M1LhdHNCVkeHUh?=
S-117 M3PrXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVv6PJZlUUN3ME2wMlkzOTNzIN88US=> MX\TRW5ITVJ?
D-247MG M1XwN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2rORmlEPTB;MD65OFg2OiEQvF2= NVnz[25EW0GQR1XS
TYK-nu NWPlS3pTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV;rR5hFUUN3ME2xMlA4QTF{IN88US=> MX;TRW5ITVJ?
A498 NVfQT5B{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4i4TmlEPTB;MT6zNFQ3QSEQvF2= NX[wVoxUW0GQR1XS
BCPAP NXPCV5BHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFnHdIxKSzVyPUGuOlY5QThizszN NG\VbHZUSU6JRWK=
KYSE-180 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3XaWGlEPTB;MT63Nlc5QCEQvF2= MoezV2FPT0WU
MIA-PaCa-2 NUfId4R4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUH0fnl2UUN3ME2xMlc2QTd5IN88US=> M2DCUHNCVkeHUh?=
HCC1395 M4HxR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHnFSVJKSzVyPUGuO|gzPzNizszN MVfTRW5ITVJ?
RS4-11 NYq5d5lTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGrtNnRKSzVyPUGuPVU1OTVizszN M2PzdXNCVkeHUh?=
NCI-H2122 M1\vXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnzBTWM2OD1{LkK3Olg1KM7:TR?= MkjyV2FPT0WU
SNU-423 Mo[4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4L3[2lEPTB;Mj60PVc5PyEQvF2= NXjDO5JoW0GQR1XS
SK-LMS-1 MmHrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVTJR|UxRTJwNkSxNlgh|ryP NX3meIg3W0GQR1XS
RPMI-7951 NH7qTWNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NImyS|BKSzVyPUKuO|AyQTNizszN M3z1TXNCVkeHUh?=
COLO-829 NUXZNWNsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlywTWM2OD1{Lke2OVQh|ryP MkL0V2FPT0WU
SCC-9 NF3jXlRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWLJR|UxRTJwOUW0PFUh|ryP M3rwVnNCVkeHUh?=
A704 NUC3O5R3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYfJR|UxRTNwMEixNkDPxE1? M4HQUHNCVkeHUh?=
HOP-62 M1LRS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1vYdmlEPTB;Mz6zOlA2PCEQvF2= MUPTRW5ITVJ?
HCC1569 M{Lze2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFflOG1KSzVyPUOuOFg{ODVizszN NG\XUZlUSU6JRWK=
A172 MkHyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHrrXFJKSzVyPUOuO|U1QThizszN M4nPUnNCVkeHUh?=
MOLT-16 M3fkemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{\3e2lEPTB;Mz64PFU2PiEQvF2= MVLTRW5ITVJ?
TE-15 NX;2W|B1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXrkUnRbUUN3ME2zMlk2OzR|IN88US=> MWjTRW5ITVJ?
OE19 MoC3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWezRmlUUUN3ME2zMlk3PDVzIN88US=> M2\H[XNCVkeHUh?=
MHH-ES-1 M1j6dGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkX0TWM2OD12LkC0OVQ2KM7:TR?= MYfTRW5ITVJ?
NKM-1 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYXYcnVXUUN3ME20MlE{QTdzIN88US=> MWDTRW5ITVJ?
RCC10RGB MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXLJR|UxRTRwMkm0O|kh|ryP MVLTRW5ITVJ?
BxPC-3 NH7DRVVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MknCTWM2OD12LkewN|kyKM7:TR?= Moe2V2FPT0WU
ALL-PO NUXGZmJLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWTJR|UxRTRwOEO4OVgh|ryP MmK1V2FPT0WU
SK-OV-3 NVLrN45zT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlriTWM2OD12Lkm5N|Qh|ryP Mn3oV2FPT0WU
SW1710 M2TQNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlnPTWM2OD13LkSyOFU3KM7:TR?= NWjSd4w3W0GQR1XS
NCI-H2452 MmnLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV;JR|UxRTVwNEW2Nlgh|ryP NInm[4VUSU6JRWK=
D-392MG MlvyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEDpNmNKSzVyPUWuOFkyPDVizszN Mn7kV2FPT0WU
LB2518-MEL MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVLvNY5XUUN3ME21MlczPjJ5IN88US=> MXPTRW5ITVJ?
BL-41 MnO5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVfIO4c6UUN3ME21MlgyOjZ4IN88US=> MVjTRW5ITVJ?
WM-115 NIDtO4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnTPTWM2OD13LkixOVA6KM7:TR?= Mk\tV2FPT0WU
SK-MEL-2 NVXqdYw4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWPwOZFOUUN3ME22MlM5OzR2IN88US=> NFvibYFUSU6JRWK=
CGTH-W-1 NYPLfnlxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYXJR|UxRTZwNEG3JO69VQ>? NUDTeYY4W0GQR1XS
BB65-RCC M37KdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVvJR|UxRTZwNUOxPVUh|ryP MWHTRW5ITVJ?
CAL-85-1 M2TXWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUfJR|UxRTZwNkC5N|Yh|ryP NVjzNJhSW0GQR1XS
RT-112 NFvlXGlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2XmbWlEPTB;Nz6xOlQ2PiEQvF2= NHPle3JUSU6JRWK=
MC116 NGrx[YZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHm1OJlKSzVyPUeuNlg3QTFizszN NYHy[3JTW0GQR1XS
G-361 M1LYTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX3JR|UxRTdwNE[1OlEh|ryP NIP6OWtUSU6JRWK=
SW1417 NXz5SYhHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGXp[WJKSzVyPUeuOlQ2QDRizszN MUXTRW5ITVJ?
MV-4-11 NEPyVWpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlzHTWM2OD16LkW0N|A1KM7:TR?= NGfhTGxUSU6JRWK=
CAL-72 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEXNZYZKSzVyPUiuO|YyOTNizszN NXjmVoNVW0GQR1XS
MKN28 NHLkZXNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{TZTGlEPTB;OD64PFUyPiEQvF2= MkK1V2FPT0WU
MEG-01 Ml;RS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2\jUmlEPTB;OD65PVA1OyEQvF2= NIq5[mhUSU6JRWK=
ACHN M{XCeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH\IOYlKSzVyPUmuN|A2PDZizszN M13CcHNCVkeHUh?=
NCI-H1755 MlHpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3PEdWlEPTB;OT6zPVk5PiEQvF2= MXPTRW5ITVJ?
NCI-H64 NHTCcoZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXTMcZlqUUN3ME25MlQzOjV3IN88US=> NVfRUY5VW0GQR1XS
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LB996-RCC NIfCZnRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkDYTWM2OD17LkWyN|k4KM7:TR?= NVTXbZNxW0GQR1XS
SK-N-DZ M{ezNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYLEfGtZUUN3ME25MlY2PzR{IN88US=> MlnkV2FPT0WU
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NCI-H28 NV;uWpR2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUHJR|UxRTFyLkS0NVMh|ryP M2[4OnNCVkeHUh?=
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HT-3 NUS3U3VKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYDPZlN5UUN3ME2xNE43OTd7IN88US=> MVzTRW5ITVJ?
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NCI-H526 M4XVemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVPOUlZKUUN3ME2xNU4yOzZ3IN88US=> M4rVO3NCVkeHUh?=
HEL M1PX[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHrLN3NKSzVyPUGxMlY2PzlizszN MVzTRW5ITVJ?
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PC-14 MnLDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWTxWWFRUUN3ME2xNk4{OjRizszN MV7TRW5ITVJ?
C8166 M{POOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnPrTWM2OD1zMz6yN|E5KM7:TR?= MmfVV2FPT0WU
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T98G NFrWTmRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2fQZmlEPTB;MUOuOFcyPyEQvF2= M3HKV3NCVkeHUh?=
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SW48 MljtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmfBTWM2OD1zMz63NlY2KM7:TR?= MnyxV2FPT0WU
MEL-HO NFrLcWFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{K5VGlEPTB;MUOuPFgzQSEQvF2= MX\TRW5ITVJ?
SF539 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmHiTWM2OD1zND6wNVE3KM7:TR?= MY\TRW5ITVJ?
NB1 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXfD[XZ[UUN3ME2xOE4yQTN3IN88US=> NIHId2RUSU6JRWK=
ST486 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGrKPGhKSzVyPUG0MlIzPTFizszN NH:0do1USU6JRWK=
LoVo MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVy5XIs{UUN3ME2xOU4zPjV7IN88US=> NFfENmtUSU6JRWK=
NH-12 NXjPdGh5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWfJR|UxRTF4LkCwPVch|ryP NEn0SoJUSU6JRWK=
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SIMA NXLoVIc1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NI\vRlRKSzVyPUG3MlkyOThizszN M{e4VnNCVkeHUh?=
8305C MonsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWXGdHRpUUN3ME2xPE42OzB3IN88US=> Mm\TV2FPT0WU
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NCI-H727 NEjrc3pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoTDTWM2OD1{MD6yNVY6KM7:TR?= Ml7NV2FPT0WU
HCC1187 MlzmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlzJTWM2OD1{MD6zOVg6KM7:TR?= NFzHdWFUSU6JRWK=
KYSE-450 MmrhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVvN[I5jUUN3ME2yNU42OzV2IN88US=> MnzKV2FPT0WU
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BPH-1 NFTscHlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1H3WWlEPTB;MkGuO|A{PSEQvF2= MYHTRW5ITVJ?
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TGBC24TKB NF;S[m1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUO0XIRqUUN3ME2yNU45QTV5IN88US=> M2fWenNCVkeHUh?=
KARPAS-299 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmDoTWM2OD1{Mj6xPVU1KM7:TR?= NFH4O5JUSU6JRWK=
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LS-513 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXTJR|UxRTJ{Lk[xPVIh|ryP MXLTRW5ITVJ?
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REH MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYLj[WVSUUN3ME2yN{4yPjl7IN88US=> MUTTRW5ITVJ?
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PANC-10-05 MnHIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHTaVXBKSzVyPUK0MlA5QThizszN NXLXWZJ1W0GQR1XS
HMV-II MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVTobppbUUN3ME2yOE4zOTZ|IN88US=> M3nFbnNCVkeHUh?=
NCI-H1963 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M37YZ2lEPTB;MkSuNlg{QSEQvF2= NIH4N3JUSU6JRWK=
LU-139 MnjES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUDJR|UxRTJ2LkizOUDPxE1? NXPldI86W0GQR1XS
KASUMI-1 M3jvXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV\LbGdkUUN3ME2yOE46OzJ6IN88US=> MoHiV2FPT0WU
RO82-W-1 MmjKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV[5N4RyUUN3ME2yOk44Ozd5IN88US=> NXzMXGtMW0GQR1XS
NCI-H1770 MoDmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYTJR|UxRTJ4Lki5PFYh|ryP M4qyTXNCVkeHUh?=
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KLE M3XBXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVPmO5ZiUUN3ME2yO{4zPTZ3IN88US=> NEnY[YRUSU6JRWK=
NCI-H2170 NXjvZVAxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV:z[ZF2UUN3ME2yO{42Ojh{IN88US=> NXfrNXNrW0GQR1XS
COLO-792 NFexTZJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3vFTGlEPTB;MkeuO|czQCEQvF2= NUjV[Gd1W0GQR1XS
ES8 NV21PHUxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUnJR|UxRTJ5Lke4NVQh|ryP Mn;nV2FPT0WU
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SU-DHL-1 NFX2eYhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVGzVXJ5UUN3ME2yO{46PDd2IN88US=> NHLySXRUSU6JRWK=
OAW-28 M1nzXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NU\MOIZrUUN3ME2yPE4xODV{IN88US=> M4[2UHNCVkeHUh?=
C32 MoT3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1HjeWlEPTB;MkiuNFgzPiEQvF2= MoPnV2FPT0WU
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JiyoyeP-2003 Moi3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3X5VGlEPTB;MkmuOlcyQSEQvF2= MWXTRW5ITVJ?
A204 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2i4Z2lEPTB;MkmuOlgzQCEQvF2= M1;idHNCVkeHUh?=
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CAKI-1 NUDibpJsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{fnNGlEPTB;M{CuNlY2PiEQvF2= M{H0OHNCVkeHUh?=
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SK-MM-2 NGHRTGlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1nTPWlEPTB;M{CuPVc6OiEQvF2= MV\TRW5ITVJ?
LB373-MEL-D NIj1NXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYrJR|UxRTNzLkCwOFch|ryP M{X3PXNCVkeHUh?=
CAS-1 Mm\uS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYXJR|UxRTNzLke2OlUh|ryP MlTIV2FPT0WU
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HCC1143 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MleyTWM2OD1|Mj63PVc6KM7:TR?= MXnTRW5ITVJ?
NCI-H1648 NI\xZYFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnnHTWM2OD1|Mj64OFY4KM7:TR?= M{O3TnNCVkeHUh?=
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KU-19-19 MlXiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUn6VXlKUUN3ME2zN{44PTN2IN88US=> NE\sUGdUSU6JRWK=
PFSK-1 NHf1ZWRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MULJR|UxRTN2LkmyOFEh|ryP NGCzNHdUSU6JRWK=
TK10 M4PDbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWjVd|JyUUN3ME2zOU4yOzJ{IN88US=> NXX3eotSW0GQR1XS
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MKN1 NETKVnFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUnQZotOUUN3ME2zOk45PDR2IN88US=> M{HvT3NCVkeHUh?=
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HCC2218 MnnmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3jGSWlEPTB;NECuOVg1QSEQvF2= Mmf4V2FPT0WU
BE-13 NGCxR4xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYLmWXNQUUN3ME20NU41PDd|IN88US=> NEfJ[lZUSU6JRWK=
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MPP-89 NYrBeIJGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH3zc2FKSzVyPUSxMlkzOTFizszN MYfTRW5ITVJ?
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HCT-116 MnrUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXLJR|UxRTR|LkKwPVkh|ryP M2CyNHNCVkeHUh?=
LB831-BLC MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXnJR|UxRTR|Lk[5O{DPxE1? NVnZ[3pGW0GQR1XS
TE-8 M4rFUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYTJR|UxRTR|Lke4PVUh|ryP MWjTRW5ITVJ?
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KYSE-270 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWrQZZdEUUN3ME20OU4xPDN5IN88US=> Mn;lV2FPT0WU
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UACC-62 MlXyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NW\VSHByUUN3ME20Ok45PjB6IN88US=> MUTTRW5ITVJ?
D-542MG MoXDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVHJR|UxRTR5LkS1PFkh|ryP MWnTRW5ITVJ?
NCI-H747 M4nFemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYnJR|UxRTR5LkeyOlQh|ryP MVfTRW5ITVJ?
HCC2998 NYj4XXhRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV65RVlGUUN3ME20PE4zQTh{IN88US=> NFLBdHhUSU6JRWK=
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COR-L105 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX\KNnRuUUN3ME20PE46PzNizszN MX3TRW5ITVJ?
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MFH-ino MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUjxOJRlUUN3ME2xN|EvOTF2IN88US=> MXvTRW5ITVJ?
HT-1080 NV\jZlgzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2PBUWlEPTB;MUOyMlIxPCEQvF2= M1zHNnNCVkeHUh?=
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NOS-1 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2nLXGlEPTB;MUO0Mlc3PSEQvF2= M{ewPHNCVkeHUh?=
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OVCAR-4 NFvuTHJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX7JR|UxRTF2MD63OFkh|ryP MnHTV2FPT0WU
Detroit562 NV3sUVdtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWj0UWRMUUN3ME2xOFEvPDZ4IN88US=> MluwV2FPT0WU
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CCF-STTG1 MnjaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXPUVnRYUUN3ME2xOFMvOzN2IN88US=> MnTjV2FPT0WU
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DMS-114 MoXHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NU\WVI84UUN3ME2xOFcvPDRzIN88US=> NIHCUJJUSU6JRWK=
GCIY MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHr1VFZKSzVyPUG0O{44QTdizszN MnHuV2FPT0WU
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KYSE-520 NYnEcGhMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1nlPWlEPTB;MUS4MlU1KM7:TR?= MVvTRW5ITVJ?
LB647-SCLC NV2yOJo{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1OyemlEPTB;MUS4MlU3OiEQvF2= M4joZ3NCVkeHUh?=
639-V Mo[xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MY\JR|UxRTF2OD63NFUh|ryP MVTTRW5ITVJ?
TE-9 M4nLNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkXyTWM2OD1zNEmuOFQ{KM7:TR?= MVPTRW5ITVJ?
Caov-3 Mo\MS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{P0dWlEPTB;MUS5MlcyPCEQvF2= NVXCcYpJW0GQR1XS
L-540 M3L4VWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4PoeWlEPTB;MUS5Mlc2KM7:TR?= Mnv0V2FPT0WU
NCI-H748 NGrPXmlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX;JR|UxRTF3MD6yNFkh|ryP MkTJV2FPT0WU
GDM-1 M{LJT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{TpZ2lEPTB;MUWxMlE{PSEQvF2= MVvTRW5ITVJ?
GAK MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVXa[odxUUN3ME2xOVEvOzd2IN88US=> MV7TRW5ITVJ?
AsPC-1 M{nKN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGPoWmhKSzVyPUG1Nk44PjRizszN MkW4V2FPT0WU
MDA-MB-361 M1vQTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVX2TJRiUUN3ME2xOVMvOTZ2IN88US=> NGr6e5RUSU6JRWK=
TT NF:2RmtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1HBUmlEPTB;MUWzMlQ6KM7:TR?= MX;TRW5ITVJ?
Calu-1 MmrhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYXJR|UxRTF3ND6wNFMh|ryP M3H0b3NCVkeHUh?=
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NCI-H2052 Ml[0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MY\JR|UxRTF3OT61PFMh|ryP NH:xTXBUSU6JRWK=
EFO-27 NHrze2dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVjJO2cyUUN3ME2xOlAvPTh2IN88US=> M3LRUnNCVkeHUh?=
CAL-62 NXruV4E2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYT6c5BZUUN3ME2xOlIvPjF|IN88US=> MXHTRW5ITVJ?
CHP-212 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MY\JR|UxRTF4Mz62NlYh|ryP NVnpUWRFW0GQR1XS
AU565 M{DqVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1TPOGlEPTB;MU[zMlY1KM7:TR?= MVTTRW5ITVJ?
Ca9-22 NH\nUWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYPJR|UxRTF4ND6zNlEh|ryP NIjkdWlUSU6JRWK=
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OVCAR-5 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoP6TWM2OD1zNkiuN|A6KM7:TR?= NF\w[pJUSU6JRWK=
Calu-3 NGj2RmpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{LKN2lEPTB;MU[4MlMyOSEQvF2= MX3TRW5ITVJ?
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ME-180 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV3UVYlwUUN3ME2xO|QvODF4IN88US=> M{j6UXNCVkeHUh?=
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NCI-H522 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{n0VGlEPTB;MUixMlA2QSEQvF2= M2rlVHNCVkeHUh?=
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NCI-H1563 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{Lm[mlEPTB;MUi1MlIxQSEQvF2= MmDsV2FPT0WU
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ETK-1 M1PaVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVvUZYZiUUN3ME2xPVAvOThzIN88US=> M3LjOHNCVkeHUh?=
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HGC-27 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUO2bI5PUUN3ME2xPVUvPTN6IN88US=> NV\RSFQ5W0GQR1XS
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SW780 NYX1[IJjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVvjZZhOUUN3ME2xPVYvPzN2IN88US=> NWW5TIt5W0GQR1XS
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JVM-3 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX7YUVZUUUN3ME2xPVgvPDF7IN88US=> MmDtV2FPT0WU
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MDA-MB-231 MmDLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4jSb2lEPTB;MkCwMlkxOiEQvF2= NYTyPZpEW0GQR1XS
MFM-223 NVjT[VhCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV\JR|UxRTJyMT61PFch|ryP MV\TRW5ITVJ?
MFE-280 NVTheoJRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWO3XXZXUUN3ME2yNFEvQTZzIN88US=> MnPMV2FPT0WU
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HCC1954 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NY\vWVBmUUN3ME2zNlMvPTh{IN88US=> MV3TRW5ITVJ?
SK-MEL-24 NXnmfHpsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYXaVlF3UUN3ME2zNlQvOTFzIN88US=> MXPTRW5ITVJ?
NTERA-S-cl-D1 NVPQZ4xMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYjvSWFUUUN3ME2zNlYvPDh7IN88US=> Mnu0V2FPT0WU
SK-MEL-30 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn63TWM2OD1|Mk[uO|M5KM7:TR?= M3TsVnNCVkeHUh?=
LS-123 NXHxPFZvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{\Oc2lEPTB;M{K4MlE3PCEQvF2= Ml\LV2FPT0WU
SF268 NHfZW|RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmOwTWM2OD1|M{CuNlY2KM7:TR?= Mn7CV2FPT0WU
BT-474 MmewS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVzJR|UxRTN|Mj60NVgh|ryP MlPiV2FPT0WU
NCI-H1522 NUjQV2pTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnvXTWM2OD1|M{SuN|M6KM7:TR?= NXXLTYRwW0GQR1XS
LC-2-ad M3vkWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mo\6TWM2OD1|M{[uOVkyKM7:TR?= MkHzV2FPT0WU
LAN-6 NIj0RplIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmG5TWM2OD1|M{[uPVc4KM7:TR?= NXrLT3JQW0GQR1XS
ARH-77 M1TwT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2XKR2lEPTB;M{O3MlMzKM7:TR?= NYrWRmxjW0GQR1XS
HuO9 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWTJR|UxRTN|Nz60O{DPxE1? NUfhWZVKW0GQR1XS
MLMA M2rrRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHLURohKSzVyPUOzPE4yPTVizszN M2XabnNCVkeHUh?=
Raji NUnXe2UyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3r2XGlEPTB;M{O4MlM5KM7:TR?= MUXTRW5ITVJ?
GT3TKB MnLZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2HiSGlEPTB;M{O4MlQyOiEQvF2= Mof0V2FPT0WU
NCI-H441 NHPvOVZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUi0eXVEUUN3ME2zOFEvPDF6IN88US=> NWniSodLW0GQR1XS
NCI-H2030 NFPqW4VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVXlZ|dSUUN3ME2zOFEvPjB7IN88US=> NUe0OpljW0GQR1XS
VM-CUB-1 M1zEcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWrkUIo{UUN3ME2zOFIvPjN4IN88US=> MUfTRW5ITVJ?
KP-4 MkGyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2PZUmlEPTB;M{SzMlc{OyEQvF2= MnTwV2FPT0WU
PF-382 MkLYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVezU5FqUUN3ME2zOFQvPDJ{IN88US=> MULTRW5ITVJ?
NB13 M{H4Nmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGP6WXZKSzVyPUO0OU42QCEQvF2= NILJOnNUSU6JRWK=
D-502MG MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnLYTWM2OD1|NEWuPFEzKM7:TR?= MkDRV2FPT0WU
CAL-54 MmTLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{HkeGlEPTB;M{S1Mlg4QSEQvF2= NUjpfYREW0GQR1XS
DOHH-2 NYPJS4F1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX[y[3BsUUN3ME2zOFYvPjVizszN MlHIV2FPT0WU
SK-PN-DW MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{LpfmlEPTB;M{S3MlQzPiEQvF2= M1T3OXNCVkeHUh?=
NCI-H720 M3zHV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoLDTWM2OD1|NEmuPFQ3KM7:TR?= NX22XXd{W0GQR1XS
SW1990 NXLwS4ZIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYjJR|UxRTN3MT6yPEDPxE1? MWDTRW5ITVJ?
NCI-H2196 M2nNSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{TzSmlEPTB;M{WyMlYzPCEQvF2= MVrTRW5ITVJ?
P30-OHK MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MofqTWM2OD1|NUOuNFY{KM7:TR?= NUPvSlZiW0GQR1XS
NCI-H358 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnfuTWM2OD1|NUWuN|U3KM7:TR?= M1f4TnNCVkeHUh?=
CW-2 M3nGRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWrJR|UxRTN3Nj61Nlkh|ryP NGHpWIJUSU6JRWK=
COLO-678 NX24WnJRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFL0ZmVKSzVyPUO1O{45PjNizszN MniyV2FPT0WU
NCI-H2126 MoLYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn3mTWM2OD1|NUiuOVIyKM7:TR?= NHPFeHdUSU6JRWK=
DMS-53 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{[4SGlEPTB;M{W4MlcyOSEQvF2= M3PSPHNCVkeHUh?=
NCI-H1838 M4G4eWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXzSOHozUUN3ME2zOVkvQTh5IN88US=> NF7DUoJUSU6JRWK=
SJSA-1 NI\hPZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{K5OmlEPTB;M{[wMlk{QSEQvF2= M1XuTHNCVkeHUh?=
ChaGo-K-1 MmjKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYO3bG9YUUN3ME2zOlMvQTJ2IN88US=> NHu5XoJUSU6JRWK=
IST-SL2 NUnpcGxqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1nvcGlEPTB;M{eyMlkzPiEQvF2= MnezV2FPT0WU
U-266 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWHJR|UxRTN5Mz6xN|Ih|ryP NXW1OFF{W0GQR1XS
P31-FUJ MlzJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV6yeFluUUN3ME2zO|YvQTZ2IN88US=> NVHmfGZyW0GQR1XS
HCC2157 NFPyUXdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlvYTWM2OD1|N{iuNVYyKM7:TR?= MmXYV2FPT0WU
COR-L88 NXnPd5Z6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4W5b2lEPTB;M{izMlY4PyEQvF2= MVrTRW5ITVJ?
NCI-H596 MmHvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFK5RXlKSzVyPUO4OU4{PCEQvF2= MYTTRW5ITVJ?
GOTO NX;ifJFRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH\NWVBKSzVyPUO5NE43PDFizszN NX\wfnVLW0GQR1XS
NCI-H719 M3vIW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXzXZopDUUN3ME2zPVEvQDl|IN88US=> M3;YS3NCVkeHUh?=
MN-60 MorYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVHieINNUUN3ME2zPVMvQTR6IN88US=> MYHTRW5ITVJ?
IST-SL1 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{DjSGlEPTB;M{m0MlQxPiEQvF2= NEDPcZdUSU6JRWK=
TE-6 NFS0Z5ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlP5TWM2OD1|OUeuNlU1KM7:TR?= NFP0VIVUSU6JRWK=
SW962 NYXlW4pTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4PId2lEPTB;M{m4MlY6KM7:TR?= MWPTRW5ITVJ?
FADU NFzq[FFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{XQPGlEPTB;NECzMlA5OyEQvF2= M3vOR3NCVkeHUh?=
ECC4 M{e4cGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVvJR|UxRTRyOD60OVgh|ryP NV3YPVVMW0GQR1XS
MES-SA NHzEdmpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{PPNmlEPTB;NEC5Mlk5KM7:TR?= NI[xbZNUSU6JRWK=
NCI-H716 Mnv6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXHJR|UxRTRzMD6wO|Uh|ryP M{XBSHNCVkeHUh?=
GR-ST NYPWVoRDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUnBdHJoUUN3ME20NVAvPDl5IN88US=> NH64eG5USU6JRWK=
KY821 M{PqU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1\CdWlEPTB;NEG0MlA5PiEQvF2= NV7VUZl3W0GQR1XS
NCI-H2141 NWDCN5RXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4S4TGlEPTB;NEK0MlM5QCEQvF2= NHnT[odUSU6JRWK=
MDA-MB-134-VI NEHUSWVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlfRTWM2OD12M{GuNFM{KM7:TR?= NHSwdYVUSU6JRWK=
RH-1 NImxVJJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXrJR|UxRTR|NT6wPUDPxE1? M2TwdnNCVkeHUh?=
AM-38 NW\6b4xkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFPTSmFKSzVyPUSzPE42PCEQvF2= MmjnV2FPT0WU
BL-70 M4HvcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXu5TZhTUUN3ME20N|kvPDJ7IN88US=> NGHUW|RUSU6JRWK=
COLO-684 NIHtZmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NU\tb4c4UUN3ME20OFgvQDh6IN88US=> NVfTWlhuW0GQR1XS
DMS-79 MlPMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXrKO4IyUUN3ME20OFkvPjhizszN M3[3bHNCVkeHUh?=
EW-1 MnfvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVfJR|UxRTR3Mj64PFgh|ryP MUTTRW5ITVJ?
MS-1 NFfrc21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGnXNFBKSzVyPUS1OE4{QDVizszN M4joV3NCVkeHUh?=
A4-Fuk NGm1ZVJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M37kRWlEPTB;NEW0MlUxPyEQvF2= NEHrSmVUSU6JRWK=
TALL-1 NXjWeJA2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWrxbFBPUUN3ME20OVUvOjl5IN88US=> M3rjN3NCVkeHUh?=
SCLC-21H MoTYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFzJSJFKSzVyPUS1OU43QTlizszN MXLTRW5ITVJ?
KMS-12-PE Mnm2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYLOe5JZUUN3ME20OVYvQDJ{IN88US=> MmPCV2FPT0WU
TE-441-T M2\ZVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH;IUHNKSzVyPUS1O{4yQDZizszN Ml\wV2FPT0WU
A3-KAW MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4DsfGlEPTB;NE[wMlg1OSEQvF2= NFS5[5BUSU6JRWK=
NCI-H1092 NGXxdJVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVXHXmVEUUN3ME20OlMvQTR|IN88US=> MUDTRW5ITVJ?
COR-L279 MnzsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2HhS2lEPTB;NE[0Mlg1PyEQvF2= NInNSoJUSU6JRWK=
NCI-SNU-5 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVfnWYhrUUN3ME20O|IvOjhizszN MULTRW5ITVJ?
JAR MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUOwN4N3UUN3ME20O|QvPzh6IN88US=> MY\TRW5ITVJ?
NCI-H446 NVjSN3ZtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1PlfWlEPTB;NEe3MlA5KM7:TR?= MonUV2FPT0WU
HDLM-2 M{HD[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYXJR|UxRTR6MD64Olkh|ryP NWLTO5V1W0GQR1XS
D-283MED NV30OmNVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmThTWM2OD12OEiuPVI1KM7:TR?= MYrTRW5ITVJ?
NCI-H1395 MnvRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4rVOWlEPTB;NEi5MlAxPCEQvF2= M{jWT3NCVkeHUh?=
RPMI-6666 NHrnfmlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml3MTWM2OD12OUGuN|k5KM7:TR?= NV;2XllyW0GQR1XS
CPC-N MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1\KOGlEPTB;NUCyMlA3OyEQvF2= NEHK[YVUSU6JRWK=
EC-GI-10 NIfKfoJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGS5UYNKSzVyPUWwNk4yPjlizszN NWPN[497W0GQR1XS
CA46 M1z2Vmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWHJR|UxRTVyND60NFch|ryP M2jRRXNCVkeHUh?=
EHEB NYHTclM3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWHJR|UxRTVyNz6yNFgh|ryP NF:zcY1USU6JRWK=
RPMI-8402 NYPxbFM6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEi5VYJKSzVyPUWxNE4xOjFizszN M3j2TXNCVkeHUh?=
DU-4475 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4jpU2lEPTB;NUGxMlExOSEQvF2= NWHBUId2W0GQR1XS
P12-ICHIKAWA NVntN4pST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmnPTWM2OD13MUGuOFE3KM7:TR?= NHHoendUSU6JRWK=
NCI-H1694 MnX2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYfJR|UxRTVzMj60N|kh|ryP M2XP[HNCVkeHUh?=
NCI-H2171 M2XuOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnSyTWM2OD13MUKuO|A2KM7:TR?= Mmr3V2FPT0WU
Mo-T M3rES2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1XRNGlEPTB;NUG1MlEyPiEQvF2= NFXC[FVUSU6JRWK=
HL-60 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkL4TWM2OD13MkKuPVI1KM7:TR?= NU\wT3djW0GQR1XS
NCI-H1436 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4nhfmlEPTB;NUKzMlA3PCEQvF2= NGr5[WhUSU6JRWK=
NCI-H510A M3TIXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVjJR|UxRTV{ND62PVYh|ryP NI\OO5lUSU6JRWK=
NCI-H2081 M2e5PWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2\ZVmlEPTB;NUK1MlUyOyEQvF2= M3vLS3NCVkeHUh?=
NB5 NIXBcY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2\OeGlEPTB;NUK2MlQ{KM7:TR?= NUn4fIpNW0GQR1XS
NCI-H2227 MkO4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml;jTWM2OD13M{CuNlU1KM7:TR?= MXnTRW5ITVJ?
HSC-4 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1\zVWlEPTB;NUO5MlI6OyEQvF2= NH3pOWlUSU6JRWK=
RL NIXSTnpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoDVTWM2OD13NEKuOFQzKM7:TR?= NUHRXVE6W0GQR1XS
EVSA-T NH3aU5BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFXuPGtKSzVyPUW0N{43QDRizszN M2PhRXNCVkeHUh?=
NCI-H889 M3ruV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWLJR|UxRTV2Nz6yN{DPxE1? M1rO[HNCVkeHUh?=
COLO-320-HSR NFvnUIJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXHJR|UxRTV3MT6zOUDPxE1? MoT3V2FPT0WU
EW-11 NHTxfJBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MorLTWM2OD13NUKuN|k6KM7:TR?= Mnn5V2FPT0WU
UACC-812 NWDa[Gk2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1XIfGlEPTB;NUW5MlM6OSEQvF2= M4fpR3NCVkeHUh?=
CAL-148 NYfPXIQyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoHXTWM2OD13NkWuNFg6KM7:TR?= MlLGV2FPT0WU
K052 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3LnXGlEPTB;NUe5MlIyOyEQvF2= NVvPU|NpW0GQR1XS
L-428 NIC1TYxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHjwNpFKSzVyPU[wNE43PzFizszN NUPIdFVpW0GQR1XS
LP-1 MlPkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoLVTWM2OD14MEOuOVg4KM7:TR?= NWH0XXJ6W0GQR1XS
ML-2 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MknpTWM2OD14ME[uOVc6KM7:TR?= NV;QVnViW0GQR1XS
KARPAS-45 NIf6boVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWfJR|UxRTZzOD6yNlEh|ryP MnrxV2FPT0WU
HT NV7xUpN1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1fTWWlEPTB;NkKyMlc5PSEQvF2= NGjB[5VUSU6JRWK=
NCI-H226 M4LhV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkjJTWM2OD14MkiuN|U{KM7:TR?= MoniV2FPT0WU
L-363 NG\VXFNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFfsWo9KSzVyPU[0PE4xPjRizszN NIXrc5VUSU6JRWK=
IM-9 NXzHWmx5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXnJR|UxRTZ3MT61OFEh|ryP NG\IN5pUSU6JRWK=
TUR MnrpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGPW[5lKSzVyPU[3NU4{OjJizszN MlWwV2FPT0WU
NCI-SNU-16 MlP4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVPJR|UxRTZ6MD60OlIh|ryP MlzOV2FPT0WU
NCI-SNU-1 MmriS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF7nUlRKSzVyPU[5NE4yPzJizszN NVjNNXF1W0GQR1XS
U-87-MG MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVjIOoJVUUN3ME22PVQvPTh3IN88US=> NY\ETlVmW0GQR1XS
KM-H2 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn;DTWM2OD14OUmuNFU6KM7:TR?= MknFV2FPT0WU
DEL MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3zQcmlEPTB;N{CwMlg{QCEQvF2= NUjN[|ZoW0GQR1XS
KARPAS-422 NH62copIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4HHcmlEPTB;N{i5MlMyPSEQvF2= MX\TRW5ITVJ?
BC-3 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXHtU2ZXUUN3ME24NFUvOjB|IN88US=> MX;TRW5ITVJ?
NCI-H1155 M3LDVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV20bpRzUUN3ME24NVgvODV7IN88US=> NV7TSnlPW0GQR1XS
NCI-H1581 NWLqO3F4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NULtRXNyUUN3ME25NFAvOjNzIN88US=> NV7obWlZW0GQR1XS

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In vivo Oral administration of CHIR-99021 at 30 mg/kg enhances glucose metabolism in a rodent model of type 2 diabetes, with a maximal plasma glucose reduction of nearly 150 mg/dl 3-4 hours after administration, while plasma insulin remains at or below control levels. Oral administration of CHIR-99021 at 16 or 48 mg/kg 1 hour before oral glucose challenges in ZDF rats significantly improves glucose tolerance with 14% and 33% reduction in plasma glucose at 16 mg/kg and 48 mg/kg, respectively, and the higher dose of CHIR-99021 also reduces hyperglycemia before the oral glucose challenge. [1] Administration of CHIR-99021 significantly augments hematopoietic repopulation in recipient mice transplanted with mouse or human hematopoietic stem cells (HSCs), suggesting that GSK-3 is a specific modulator of HSC activity. [4]

Protocol

Kinase Assay:

[1]

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Cell-free kinase assays:

Polypropylene 96-well plates are filled with 300 μL/well buffer (50 mM tris HCl, 10 mM MgCl2, 1 mM EGTA, 1 mM dithiothreitol, 25 mM β-glycerophosphate, 1 mM NaF, 0.01% BSA, pH 7.5) containing 27 nM GSK-3α or 29 nM GSK-3β, and 0.5 μM biotin-CREB peptide substrate. Various concentrations of CHIR-99021 are added in 3.5 μL of DMSO, followed by 50 μL of ATP stock to yield a final concentration of 1 μM ATP in all cell-free assays. After incubation, triplicate 100-μL aliquots are transferred to Combiplate eight plates containing 100 μL/well 50 μM ATP and 20 mM EDTA. After 1 hour, the wells are rinsed five times with PBS, filled with 200 μL of scintillation fluid, sealed, left 30 minutes, and counted in a scintillation counter. All steps are performed at room temperature.
Cell Research:

[3]

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  • Cell lines: INS-1E
  • Concentrations: Dissolved in DMSO, final concentrations ~ 20 μM
  • Incubation Time: 1, or 4 days
  • Method:

    Cells are maintained for 24 hours in starvation medium (culture medium with only 5 mM glucose, 1% fetal calf serum). Then cells are exposed to various concentrations of CHIR-99021 for 1, or 4 days. Cell number is measured by staining of cellular DNA with CyQuant dye, which becomes fluorescent when bound to DNA. Fluorescence is measured after 30 minutes of incubation using the FLUOstar Optima reader. Cell replication is determined by BrdUrd incorporation. BrdUrd labeling solution is added to the medium for the last 4 hours before the cells are fixed using FixDenat solution and incubated with monoclonal anti-BrdUrd-POD antibodies. After substrate solution is added to each well, the light emission is measured in a microplate luminometer using the Analyst HT detection system.


    (Only for Reference)
Animal Research:

[1]

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  • Animal Models: Female db/db mice or male ZDF rats with type 2 diabetes
  • Formulation: Formulated as solutions in 20 mM citrate-buffered 15% Captisol or as fine suspensions in 0.5% carboxymethylcellulose.
  • Dosages: ~48 mg/kg
  • Administration: Orally
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 93 mg/mL (185.33 mM)
Ethanol 2 mg/mL (3.98 mM)
Water Insoluble
In vivo Add solvents individually and in order:
5% DMSO+30% PEG 300+ddH2O
24mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 501.8
Formula

C22H18Cl2N8.HCl

CAS No. 1797989-42-4
Storage powder
Synonyms N/A

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Frequently Asked Questions

  • Question 1:

    I recently purchased CHIR 99021 to inject mice intraperitoneally. The data sheet says using 10% captisol for in vivo use. Is it for IP injection? Or do you have other solutions to suggest to use for in vivo IP injection of CHIR 99021?

  • Answer:

    The formulation of 15% Captisol is for oral gavage, and S2924 CHIR-99021 (CT99021) HCl in it is a suspension. S2924 CHIR-99021 (CT99021) HCl can be dissolved in 5% DMSO+30% PEG 300+ddH2O at 24mg/ml as a clear solution for IP injection.

  • Question 2:

    How about the half-life of this compound? If it is necessary for daily treatment when the compound is used in long-term experiment?

  • Answer:

    Please change the culture medium each day to add the new comound in it if the duration is more than 72h.

  • Question 3:

    What is the difference between S2924 and S1263? Which one is better for differentiation?

  • Answer:

    S1263 is the free base format of Chir-99021, while S2924 is the HCl salt format. They have the same biological activity but are slightly different in solubility.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID