CHIR-99021 (CT99021) HCl

Catalog No.S2924

CHIR-99021 (CT99021) HCl Chemical Structure

Molecular Weight(MW): 501.8

CHIR-99021 HCl (CT99021) is hydrochloride of CHIR-99021, which is a GSK-3α/β inhibitor with IC50 of 10 nM/6.7 nM; CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs Cdc2 and ERK2.

Size Price Stock Quantity  
In DMSO USD 340 In stock
USD 90 In stock
USD 170 In stock
USD 570 In stock
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Cited by 70 Publications

9 Customer Reviews

  • Differentiation induced by treatment with the Gsk3 inhibitor in hPSCs is β-catenin dependent. H9-7TGP cells were treated with 12 uM CH in mTeSR1 for 4 d. Immunofluorescent staining for Oct4, Isl1, and Nkx2.5 was compared with GFP expression. (Scale bars, 50 uM.)

    Proc Natl Acad Sci U S A 2012 109(27):E1848-57. CHIR-99021 (CT99021) HCl purchased from Selleck.

    Gpc4 regulates ESC response to Wnt/β-catenin signaling.  (E) Quantitative analysis of control and Gpc4-mutant beating foci exposed to the GSK3 inhibitor, CHIR 99021 (1 and 3 μM, from day 0 to day 5). Analysis was done at different time points. Gpc4- mutant cells treated with CHIR 99021 behave as controls (mean±SEM; n=2).

    Stem Cells 2012 30, 1863-1874. CHIR-99021 (CT99021) HCl purchased from Selleck.

  • Inhibition of GSK3 activity induces an autophagic response in human pancreatic cancer cells. PANC1 cells were treated for 24 h with DMSO, CHIR99021 (CHIR; 5 uM) or the mTOR inhibitor Torin1 (250 nM). Autophagosome (punctate LC3B) (D) and lysosome (E) labeling was visualized with a Zeiss LSM700 confocal microscope. Images were acquired with a 20x (D) or 63x (E) objective. Nuclei were stained with DAPI. Scale bars, 20 祄.

    J Biol Chem 2015 290(9), 5592-605 . CHIR-99021 (CT99021) HCl purchased from Selleck.

     

    Characterization of rES cells. A: image of normal rES cell colonies on feeder layers. B: rES colonies were positive for AP staining. CeE: rES colonies readily expressed pluripotent markers, Sox2 (C), Oct4 (D), and SSEA-1 (E). Blue, DAPI. Scale bars: 100 um.

    J Genet Genomics 2012 39, 643e651. CHIR-99021 (CT99021) HCl purchased from Selleck.

  •  

    Smad linker phosphorylation involves GSK3 in human melanoma lines. Inhibition of Smad2 and Smad3 linker phosphorylation in the presence of the specific GSK3 specific inhibitor, CT99021. 24 hours post seeding, WM793 and 1205LU cells were serum-starved for about 16 hours, and incubated in the absence (2) or presence (+) of 2 uM of CT99021 for two hours. Immunoblots were performed as in A. p: Phosphorylated. S: Serine; T: Threonine.

    PLoS One 2012 7, e47312. CHIR-99021 (CT99021) HCl purchased from Selleck.

    A. Vero cells were pretreated with the indicated concentrations of CHIR-99021 for 1 h and then infected with PEDV (MOI = 1) for 8 or 10 h. Cell lysates were prepared and analyzed using Western blotting.

    PLoS One, 2016, 11(8):e0161508. CHIR-99021 (CT99021) HCl purchased from Selleck.

  • SKOV3/DDP cells were treated with a range of concentration of (A) CHIR-99021 or in combination with 20 µM cisplatin for 48 h.

    Exp Ther Med, 2016, 12(6):3851-3858. CHIR-99021 (CT99021) HCl purchased from Selleck.

     

    PANC1 cells were treated with GSK3 inhibitors SB216763 (SB 10µM or SB 20µM) or CHIR99021 (CHIR 5µM) for 72h.

    CHIR-99021 (CT99021) HCl purchased from Selleck.

  •  

     Activity of canonical WNT/ β -CATENIN signals induced by CHIR 99021 in the developing human lung. Human lung tissue explants obtained at 15 weeks of gestation were exposed to 0, 5 and 10 μM CHIR 99021 in vitro for 72 h. (A-B) Western blot analysis showed expression of β -CATENIN in human lung tissues. Results are presented as the mean ± sem of three independent experiments. *P < 0.05, **P < 0.01. (C) qRT-PCR analysis detected a dose-dependent increase in WNT signaling transcription factors and target genes following exposure to CHIR 99021. Results are presented as the mean ± sem of three independent experiments. *P < 0.05, **P < 0.01. (D-K) Histology examination showed obvious morphogenetic changes of the airway epithelial cells associated with CHIR 99021 treated lung tubes. Scale bars: D-G =100 μm; H-K = 20 μm.

    CHIR-99021 (CT99021) HCl purchased from Selleck.

Purity & Quality Control

Choose Selective GSK-3 Inhibitors

Biological Activity

Description CHIR-99021 HCl (CT99021) is hydrochloride of CHIR-99021, which is a GSK-3α/β inhibitor with IC50 of 10 nM/6.7 nM; CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs Cdc2 and ERK2.
Targets
GSK-3β [1]
(Cell-free assay)
GSK-3α [1]
(Cell-free assay)
6.7 nM 10 nM
In vitro

CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs CDC2 and ERK2, as well as other protein kinases. Furthermore, CHIR-99021 shows only weak binding to a panel of 22 pharmacologically relevant receptors and little inhibitory activity against a panel of 23 nonkinase enzymes. CHIR-99021 induces the activation of glycogen synthase (GS) in insulin receptor-expressing CHO-IR cells with EC50 of 0.763 μM. [1] In addition to simulating the actions of insulin, inhibition of GSK-3 by CHIR-99021 (3 μM) increases free cytosolic β-catenin by 1.9-fold, mimicking the canonical Wnt signaling pathway in 3T3-L1 preadipocytes. During any of the first 3 days of differentiation, CHIR-99021 treatment inhibits the preadipocyte differentiation with IC50 of 0.3 μM by blocking induction of CCAAT/enhancer-binding protein α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ). [2] Unlike lithium chloride and AR-A014418, CHIR-99021 treatment does not reduce the viability of INS-1E cells even at high concentrations. Instead, CHIR-99021 robustly increases the rate of proliferation of INS-1E cells in a dose-dependent manner, and significantly inhibits INS-E cell death induced by high glucose and high palmitate in a concentration-dependent manner. CHIR-99021 promotes primary beta cell replication in isolated rat islets at concentrations as low as 1 μM, with 2-3 fold increase of cell replication at 5 μM of CHIR-99021 treatment. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
3T3-L1 M4\kfGZ2dmO2aX;uJGF{e2G7 NY\WS4NOOyEQvF2= MUS0JIg> NF;tO3FFVVOR MonrTY5pcWKrdIOgdJJm[WSrcH;jfZRmKGSrZn\ldoVvfGmjdHnvci=> NGfNOJoyOjB3NUKwNC=>
insulin receptor–expressing CHO-IR M4LVS2Z2dmO2aX;uJGF{e2G7 MkDOOVAxKG6P NFXIWYw{OCCvaX6= NWn0bJVITE2VTx?= M2KyOWFkfGm4YYTld{BIdHmlb3flckBUgW62aHHz[UApT1Nr NXm4SnVUOTJ4ME[0PVc>
H4IIE M1radWZ2dmO2aX;uJGF{e2G7 NHjlRYkyOCEQvF2= MWWyOEBp M3vHUWROW09? MkDKVoVofWyjdHXzJO6zNWOjdHXubY4h[WO2aY\peJkh[W6mIGTJVmUh[2:wdHHpcolv\yCycn;tc5RmeiCjY4Tpeol1gQ>? MlThNVU{PTBzOUW=
Oct4/Sox2/Klf4-infected MEFs MX3GeY5kfGmxbjDBd5NigQ>? MWixNEDPxE1? NYHJelF5OjhiZB?= MlTkSG1UVw>? NYTUeYY6WHKxbX;0[ZMhVUWIczDy[ZBzd2e{YX3tbY5oKFS{YX7z[JVk\WRiYomgU4N1PCxiU3;4Nkwh[W6mIFvs[lQ> NUj3T21NOTl6M{mwOVU>
Sca-1+ HPC NV7EZpJtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXS2PJlGOiEQvF2= NH3BWVU3KGR? NHy4fmdFVVOR M4rQXnN6dmW{Z3nzeIlk[WyueTDwdo9ud3SnczDTZ4EuOSwEoFjQR{Bxem:uaX\ldoF1cW:wIHHu[EBkd2yxbomg[o9zdWG2aX;uJJRw\2W2aHXyJJdqfGhiRVfG MVqyNVYzPDN2OB?=
HMC1.2 NGi4XnRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIjvcZc{OCEQvF2= NHjsO4g4OiCq NGrEVlBFVVOR MUfS[YR2[2W|IHPlcIwhe3W{dnn2ZYw> NYmxT4VUOjJyM{mzNFE>
Endothelial cell(EC) M3i4c2Z2dmO2aX;uJGF{e2G7 NFTUeYk{KM7:TR?= NXfmeVhDTE2VTx?= NIXIOWtRem:vb4Tld{BmdmSxdHjlcIlidCClZXzsJEhGSyliZHnm[oVz\W62aXH0bY9v MV[yNlA2OjByNh?=
PANC1 NIrXS5pCeG:ydH;zbZMhSXO|YYm= M1:1deKhPSEQvF2= M{PjUFczKGh? MXHEUXNQ MXrUdolo\2W{czDhckBieG:ydH;0bYMhemW|cH;ud4U> MUGyNlIxOTF6Nh?=
INS-1E MUTGeY5kfGmxbjDBd5NigQ>? NV31Z5dGOi53IN88US=> NGriSnczPCCq NF3Ue|VFVVOR NWrtZ|RIUW6mdXPld{BiKHKnZIXjeIlwdiCxZjDOVFEhd3[ncnX4dJJme3Orb36= M1XzUFIzPDJ5N{C0
hiPSC-NPs M1j5S2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXuz5qCK|ryP NITD[4E4OiCq MlzTSG1UVw>? NUDjPINSUW6lcnXhd4V{KGOnbHygdJJwdGmoZYLheIlwdsLi MlvhNlI3PDJ4OEe=
platelets MnPrSpVv[3Srb36gRZN{[Xl? Moq1NVAh|ryP NITJc3cyPSCvaX6= NUOxS|ZyTE2VTx?= M1\aNmlv[3KnYYPld{B1cHKxbXLpck1u\WSrYYTl[EBxdGG2ZXzleEBi\2e{ZXfheIlwdiCjbnSgRXRRKHOnY4LleIlwdg>? NXLnVWtLOjN{M{m4O|c>
DN3 MUPGeY5kfGmxbjDBd5NigQ>? NIi4N2EyOCEQvF2= MkPBPVYhcA>? MkSwSG1UVw>? NWPDbpZpUW6lcnXhd4V{KGOnbHyg[IV3\WyxcH3lcpQh[W6mIIDyc4xq\mW{YYTpc44> NF7qVYkzOzV{Nkm4PS=>
embryonic stem cells (ESC) MlXISpVv[3Srb36gRZN{[Xl? NEjp[JE{KML3TR?= MnvmNVEh\A>? NYXwSYJPTE2VTx?= MY\Qdo9ud3SnczDz[YxnNXKnbnX3ZYwhd2ZibnJDs5ZmNXS7cHWgcY92e2ViZX3idplwdmmlIIP0[Y0h[2WubIOgLGVUSyl? NHvEOHMzOzh2M{OxNi=>
H460 NHvXPFVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVO1JOK2VQ>? M1j2dFI1KGh? NF6wSJJFVVOR MmXNVJJwdW:2ZYOgZ4VtdCCycn;sbYZmemG2aX;u MYWyOFM3PzZ6OB?=
H157 NELyVopIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkjPOUDDvU1? NHPkbJUzPCCq MYjEUXNQ MkXLVJJwdW:2ZYOgZ4VtdCCycn;sbYZmemG2aX;u M1KyUlI1OzZ5Nki4
H460 Mm\2RZBweHSxc3nzJGF{e2G7 NX21OGg1PSEEtV2= Ml7yNlQhcA>? M33iOWROW09? NFjaVVFCfHSnboXheIV{KGOnbHygZZBweHSxc3nz NXH6S2V4OjR|Nke2PFg>
H157 MYTBdI9xfG:|aYOgRZN{[Xl? NUDHNJZ2PSEEtV2= NUTlSW9zOjRiaB?= NY[0THJ7TE2VTx?= NI\xNVFFd2W|IH7veEB{cWewaX\pZ4FvfGy7IHHm[oVkfCCjcH;weI9{cXN? MmT5NlQ{Pjd4OEi=
A549 NVjpV2pNSXCxcITvd4l{KEG|c3H5 NW\kNFBNPSEEtV2= M2H5fVI1KGh? MYnEUXNQ NHHib4RKdmO{ZXHz[ZMh[2WubDDhdI9xfG:|aYO= MUGyOFM3PzZ6OB?=
Neural progenitor cells NFz2OlBHfW6ldHnvckBCe3OjeR?= NWexO|liOyEQvF2= M4K5PVExKGR? NGD6W2RFVVOR NVGwe3p[UW6mdXPld{Bo\W6ncnH0bY9vKG:oIH7leZJidCCycn;n[Y5qfG:{IHPlcIx{ MWmyOFY{QDB|NB?=
ES-D3 Mn3xR5l1d3SxeHnjJGF{e2G7 MYWxNOKh|ryP MUezJIQ> NVrGelBkTE2VTx?= MlnlTY5lfWOnczDtc4RmemG2ZTDjfZRwfG:6aXPpeJkhf2m2aDDJR|UxKG:oIESuPUDPxE1? M1W3O|I1Pzd7M{[1
ES-CCE MlzOR5l1d3SxeHnjJGF{e2G7 M1fOR|ExyqEQvF2= NXTlNlRMOyCm M33kemROW09? M1XVc2lv\HWlZYOgcY9l\XKjdHWgZ5l1d3SxeHnjbZR6 MXWyOFc4QTN4NR?=
ES-D3 MVTGeY5kfGmxbjDBd5NigQ>? NWnGSoVDPcLizszN MmnuN{Bl NGSzZlRFVVOR NYD0XJlSW3S{b37ncJkhcW6mdXPld{B1cGViYXP0bZZqfHlib3[gW451N2KndHGtZ4F1\W6rbjDwZZRpf2G7 NGr4boUzPDd5OUO2OS=>
ES-CCE MYPGeY5kfGmxbjDBd5NigQ>? MoXXOeKh|ryP M1y5TVMh\A>? MUHEUXNQ MYnTeJJwdmeueTDpcoR2[2W|IITo[UBi[3Srdnn0fUBw\iCZboSvZoV1[S2lYYTlcolvKHCjdHj3ZZk> MUOyOFc4QTN4NR?=
SW982 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVXUSJdLUUN3ME2wMlIyPDF4IN88US=> NVzWV|MyW0GQR1XS
GCT NGHDPWVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX\JR|UxRTBwM{C2O|Ih|ryP NYKxcFB6W0GQR1XS
769-P MkXTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlzwTWM2OD1yLkW4OFk6KM7:TR?= MnHJV2FPT0WU
LOXIMVI NIHCenZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmTUTWM2OD1yLkW4PFMzKM7:TR?= NHXpVYFUSU6JRWK=
D-336MG MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXzEfnJbUUN3ME2wMlg1PjJizszN M1W4cnNCVkeHUh?=
S-117 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXW2TVVRUUN3ME2wMlkzOTNzIN88US=> MX7TRW5ITVJ?
D-247MG NHKzNVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUXJR|UxRTBwOUS4OVIh|ryP MnTLV2FPT0WU
TYK-nu MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYHzcpJwUUN3ME2xMlA4QTF{IN88US=> MUjTRW5ITVJ?
A498 MmfKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEX6dnZKSzVyPUGuN|A1PjlizszN MkPHV2FPT0WU
BCPAP MmrtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHO2XlFKSzVyPUGuOlY5QThizszN NFzuRVdUSU6JRWK=
KYSE-180 NUj2Z3E4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlX6TWM2OD1zLkeyO|g5KM7:TR?= NHTVNGpUSU6JRWK=
MIA-PaCa-2 Mle5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2G5NWlEPTB;MT63OVk4PyEQvF2= NE\tcm1USU6JRWK=
HCC1395 NIXVbXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXHJR|UxRTFwN{iyO|Mh|ryP NH7Fc3FUSU6JRWK=
RS4-11 M4LTW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXzTWWxUUUN3ME2xMlk2PDF3IN88US=> MXLTRW5ITVJ?
NCI-H2122 M3PIbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV7JR|UxRTJwMke2PFQh|ryP NIPufHVUSU6JRWK=
SNU-423 MoHpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEPJUGRKSzVyPUKuOFk4QDdizszN MY\TRW5ITVJ?
SK-LMS-1 NF[1bXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2rMdGlEPTB;Mj62OFEzQCEQvF2= M1zQT3NCVkeHUh?=
RPMI-7951 MoLLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUTHSnhrUUN3ME2yMlcxOTl|IN88US=> MXfTRW5ITVJ?
COLO-829 M33tRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXTufpQ5UUN3ME2yMlc3PTRizszN M2r2cnNCVkeHUh?=
SCC-9 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXXJR|UxRTJwOUW0PFUh|ryP NHfTSI9USU6JRWK=
A704 NGjhZYdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn6wTWM2OD1|LkC4NVIh|ryP MoPWV2FPT0WU
HOP-62 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYDUWnM6UUN3ME2zMlM3ODV2IN88US=> NUDlXGhVW0GQR1XS
HCC1569 NVXqVYlST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{ez[WlEPTB;Mz60PFMxPSEQvF2= M4noPHNCVkeHUh?=
A172 MmTqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX7JR|UxRTNwN{W0PVgh|ryP MX\TRW5ITVJ?
MOLT-16 M4r2dWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NILrZ5FKSzVyPUOuPFg2PTZizszN NYnDeYZqW0GQR1XS
TE-15 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkHvTWM2OD1|Lkm1N|Q{KM7:TR?= NW\YVnFmW0GQR1XS
OE19 Mn[4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGHVc2RKSzVyPUOuPVY1PTFizszN M4m4RnNCVkeHUh?=
MHH-ES-1 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MljJTWM2OD12LkC0OVQ2KM7:TR?= MnP0V2FPT0WU
NKM-1 MmTES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVvJR|UxRTRwMUO5O|Eh|ryP NGqyO3pUSU6JRWK=
RCC10RGB M37SZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVTJR|UxRTRwMkm0O|kh|ryP M1n1VXNCVkeHUh?=
BxPC-3 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYLJR|UxRTRwN{CzPVEh|ryP MX3TRW5ITVJ?
ALL-PO MkXrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYHsNJBsUUN3ME20Mlg{QDV6IN88US=> M4i2OXNCVkeHUh?=
SK-OV-3 NGrDeFBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUHzTYd5UUN3ME20Mlk6OzRizszN MoW5V2FPT0WU
SW1710 NWDWdW1IT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHntT45KSzVyPUWuOFI1PTZizszN M{\U[3NCVkeHUh?=
NCI-H2452 NUHTXYdZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnjHTWM2OD13LkS1OlI5KM7:TR?= M3TaOHNCVkeHUh?=
D-392MG MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoW3TWM2OD13LkS5NVQ2KM7:TR?= NIDsUpFUSU6JRWK=
LB2518-MEL M4fn[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnH1TWM2OD13LkeyOlI4KM7:TR?= NF7MRYlUSU6JRWK=
BL-41 NF:3VVFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1\RN2lEPTB;NT64NVI3PiEQvF2= NHvlOYNUSU6JRWK=
WM-115 M2PvU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3vnfWlEPTB;NT64NVUxQSEQvF2= Mn\5V2FPT0WU
SK-MEL-2 NULIUGF1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVnJR|UxRTZwM{izOFQh|ryP NWTaU4Y5W0GQR1XS
CGTH-W-1 M3fGPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYPJR|UxRTZwNEG3JO69VQ>? M3PIXHNCVkeHUh?=
BB65-RCC NHTLZ2hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NILE[WFKSzVyPU[uOVMyQTVizszN M1fxWXNCVkeHUh?=
CAL-85-1 NVnJT3FiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX35T3RMUUN3ME22MlYxQTN4IN88US=> NInOZoxUSU6JRWK=
RT-112 M{TlVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFzQbXlKSzVyPUeuNVY1PTZizszN NGrBOGVUSU6JRWK=
MC116 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHXPUXVKSzVyPUeuNlg3QTFizszN MkfuV2FPT0WU
G-361 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmnSTWM2OD15LkS2OVYyKM7:TR?= MYPTRW5ITVJ?
SW1417 NGXSPVVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUmzRm1FUUN3ME23MlY1PTh2IN88US=> MWHTRW5ITVJ?
MV-4-11 M2nwTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3vBcWlEPTB;OD61OFMxPCEQvF2= MVrTRW5ITVJ?
CAL-72 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVvNNGxSUUN3ME24Mlc3OTF|IN88US=> NHyzfHNUSU6JRWK=
MKN28 M2fRZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MULJR|UxRThwOEi1NVYh|ryP M2rxOXNCVkeHUh?=
MEG-01 NIHIdmhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIfjcWhKSzVyPUiuPVkxPDNizszN MVHTRW5ITVJ?
ACHN M4jMfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlrDTWM2OD17LkOwOVQ3KM7:TR?= MWXTRW5ITVJ?
NCI-H1755 NYLTXmpjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXrLPI1GUUN3ME25MlM6QTh4IN88US=> NFXx[2JUSU6JRWK=
NCI-H64 MlLES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1LVcWlEPTB;OT60NlI2PSEQvF2= M3zGeXNCVkeHUh?=
COLO-205 Ml\pS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUXjT5BOUUN3ME25MlQ{QDJzIN88US=> NU\6PHR4W0GQR1XS
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NCI-H1963 MljDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoezTWM2OD1{ND6yPFM6KM7:TR?= MUfTRW5ITVJ?
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HEC-1 NYPFTmxiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEnuOG1KSzVyPUGyPU4yQThizszN MmHzV2FPT0WU
LU-99A MnPBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{i2NWlEPTB;MUK5Mlk{OSEQvF2= Mkf4V2FPT0WU
VMRC-RCZ MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlnsTWM2OD1zM{CuN|c2KM7:TR?= NFz3bGtUSU6JRWK=
MFH-ino NE\4NFVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYPJR|UxRTF|MT6xNVQh|ryP MknGV2FPT0WU
HT-1080 M4rXXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVHJR|UxRTF|Mj6yNFQh|ryP M3j6[3NCVkeHUh?=
CTB-1 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mlq1TWM2OD1zM{KuPFk4KM7:TR?= NWLzfFlxW0GQR1XS
Capan-2 MmHVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mm\NTWM2OD1zM{OuPVc2KM7:TR?= MoXuV2FPT0WU
BB30-HNC Mn7NS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn35TWM2OD1zM{SuNVE3KM7:TR?= NUHtUWM4W0GQR1XS
CP50-MEL-B M321fmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEDXZZBKSzVyPUGzOE4zKM7:TR?= MVfTRW5ITVJ?
JEG-3 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1TZXmlEPTB;MUO0MlM2QSEQvF2= NXzJbFJMW0GQR1XS
NCI-H1437 M2nRPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NInvSFNKSzVyPUGzOE42QSEQvF2= NYPH[WpqW0GQR1XS
NOS-1 NUjFPFhRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYfuUHozUUN3ME2xN|QvPzZ3IN88US=> M{DwWXNCVkeHUh?=
G-402 NWnPNIY3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVPEZYJwUUN3ME2xN|UvOjl3IN88US=> Mn;YV2FPT0WU
OAW-42 M2DYc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXLJR|UxRTF|Nz64OVYh|ryP NVHW[IIzW0GQR1XS
Ca-Ski MnHXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH7DR2hKSzVyPUGzPE45OzFizszN NWfWfpRCW0GQR1XS
BHT-101 NFroXY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYPJR|UxRTF2MD6yNFgh|ryP M1P0c3NCVkeHUh?=
OVCAR-4 MnfLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1XqOWlEPTB;MUSwMlc1QSEQvF2= NHTub5pUSU6JRWK=
Detroit562 M3myU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF21RVBKSzVyPUG0NU41PjZizszN MnLlV2FPT0WU
OCI-AML2 Ml30S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mo[4TWM2OD1zNEGuOVA4KM7:TR?= MW\TRW5ITVJ?
OE33 M4jOe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXTYe|ByUUN3ME2xOFEvPzl6IN88US=> NFnidmdUSU6JRWK=
YKG-1 NU\LfnlbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4LHV2lEPTB;MUSyMlg5QSEQvF2= MWPTRW5ITVJ?
CCF-STTG1 NXT4dGVZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWHJR|UxRTF2Mz6zN|Qh|ryP NF\IN3NUSU6JRWK=
NCI-H810 MmHES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlrVTWM2OD1zNEOuOlg4KM7:TR?= NXK5e2M3W0GQR1XS
SCC-25 M3jERWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlfRTWM2OD1zNESuNFA2KM7:TR?= MmW3V2FPT0WU
HTC-C3 M4TwR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnTYTWM2OD1zNEWuNFc2KM7:TR?= M4T6RnNCVkeHUh?=
G-401 M2P2NWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3XUXWlEPTB;MUS1Mlg{PSEQvF2= MmHjV2FPT0WU
OMC-1 NH7CbWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYKxUW5KUUN3ME2xOFUvQTZ5IN88US=> MXLTRW5ITVJ?
LK-2 NGn2VotIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWjHbGxTUUN3ME2xOFcvOTR|IN88US=> NX3yU3g1W0GQR1XS
SW13 NHj2No5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYXJR|UxRTF2Nz6yOVMh|ryP M2\n[nNCVkeHUh?=
BB49-HNC MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NY\6cW4yUUN3ME2xOFcvPDB2IN88US=> NUDiTlA1W0GQR1XS
DMS-114 MnL4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVLtOY1uUUN3ME2xOFcvPDRzIN88US=> NHvtSVJUSU6JRWK=
GCIY M173T2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2rOW2lEPTB;MUS3Mlc6PyEQvF2= MX\TRW5ITVJ?
GAMG MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWTKOHc4UUN3ME2xOFgvOjR5IN88US=> MnrrV2FPT0WU
KYSE-520 NIm5XlhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIHr[pVKSzVyPUG0PE42PCEQvF2= MX3TRW5ITVJ?
LB647-SCLC MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmH2TWM2OD1zNEiuOVYzKM7:TR?= MUTTRW5ITVJ?
639-V MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVPmdWk6UUN3ME2xOFgvPzB3IN88US=> Mnj6V2FPT0WU
TE-9 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIXX[FJKSzVyPUG0PU41PDNizszN MVnTRW5ITVJ?
Caov-3 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NETHfItKSzVyPUG0PU44OTRizszN MYrTRW5ITVJ?
L-540 MlH6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVfJR|UxRTF2OT63OUDPxE1? NWXicG9oW0GQR1XS
NCI-H748 M{jrXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX3JR|UxRTF3MD6yNFkh|ryP NGrO[4ZUSU6JRWK=
GDM-1 NYnCZWN2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NITNPYNKSzVyPUG1NU4yOzVizszN MXLTRW5ITVJ?
GAK MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MonlTWM2OD1zNUGuN|c1KM7:TR?= NWDCSpkyW0GQR1XS
AsPC-1 NEXPcFdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFjPWmNKSzVyPUG1Nk44PjRizszN Mn7jV2FPT0WU
MDA-MB-361 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NW\JSGlZUUN3ME2xOVMvOTZ2IN88US=> MWDTRW5ITVJ?
TT NYrofVE5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1[2[mlEPTB;MUWzMlQ6KM7:TR?= NVjXTFJNW0GQR1XS
Calu-1 M1vjT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmPJTWM2OD1zNUSuNFA{KM7:TR?= M3\w[HNCVkeHUh?=
RERF-LC-MS MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXradYJGUUN3ME2xOVQvPzd{IN88US=> MlXhV2FPT0WU
DK-MG MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4fMS2lEPTB;MUW1MlE5OyEQvF2= NG[0cYdUSU6JRWK=
CAL-120 M2nXUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXzve3JrUUN3ME2xOVUvQDJzIN88US=> M3fJN3NCVkeHUh?=
HCC1419 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWj1eVBtUUN3ME2xOVYvODd5IN88US=> MmrOV2FPT0WU
DG-75 NEfYcZZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUDJR|UxRTF3Nj62PVQh|ryP MnrrV2FPT0WU
HCC38 Mk\0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV3NVmxkUUN3ME2xOVcvOzZ7IN88US=> NUPhRZVVW0GQR1XS
HCC70 NUPQclNST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mlv3TWM2OD1zNUmuNlQzKM7:TR?= MYTTRW5ITVJ?
ES4 M33We2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEnEdVBKSzVyPUG1PU4{QTZizszN NIXyS2pUSU6JRWK=
NCI-H2052 NVjBNHJtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmLSTWM2OD1zNUmuOVg{KM7:TR?= M1TmbXNCVkeHUh?=
EFO-27 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYjCdItbUUN3ME2xOlAvPTh2IN88US=> M3vVdHNCVkeHUh?=
CAL-62 MnvVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH3CR45KSzVyPUG2Nk43OTNizszN M13J[nNCVkeHUh?=
CHP-212 NF3tb2dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVLJR|UxRTF4Mz62NlYh|ryP NF3rWWlUSU6JRWK=
AU565 M1\ZNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmPmTWM2OD1zNkOuOlQh|ryP Mn;iV2FPT0WU
Ca9-22 NIHEN45Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoixTWM2OD1zNkSuN|IyKM7:TR?= NU\LOWZTW0GQR1XS
EFO-21 M3\ufGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NE\6O3hKSzVyPUG2OU41PSEQvF2= M3jJbnNCVkeHUh?=
KINGS-1 NGfNTVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUH4dlEzUUN3ME2xOlcvOzV2IN88US=> NEHxSZlUSU6JRWK=
JVM-2 NEXIToxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MorVTWM2OD1zNkeuOFUyKM7:TR?= NYLhNoJMW0GQR1XS
OVCAR-5 MoP5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHKxUY9KSzVyPUG2PE4{ODlizszN M1fESnNCVkeHUh?=
Calu-3 Moj3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX7KVXdtUUN3ME2xOlgvOzFzIN88US=> MWHTRW5ITVJ?
CP66-MEL MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV;Nbm46UUN3ME2xOlgvPDF3IN88US=> NVnjSI5oW0GQR1XS
KYSE-150 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4W1dWlEPTB;MU[5MlY{PiEQvF2= NFjNZpJUSU6JRWK=
Daoy NVzQSG9HT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkPaTWM2OD1zNkmuPFkyKM7:TR?= NXXHd3RUW0GQR1XS
HUTU-80 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3r2cGlEPTB;MUexMlA{OiEQvF2= M3LDdHNCVkeHUh?=
SW620 MnLmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXfEU3hYUUN3ME2xO|EvPTN3IN88US=> MXjTRW5ITVJ?
HuO-3N1 M4HGSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M17p[2lEPTB;MUeyMlI4OyEQvF2= M4i2UHNCVkeHUh?=
HH MmXsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmTzTWM2OD1zN{OuPFQ5KM7:TR?= NY\6XpFDW0GQR1XS
ME-180 NYHzNoZuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHLyPXNKSzVyPUG3OE4xOTZizszN NWXYcpV3W0GQR1XS
SW756 MnnGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHjydIdKSzVyPUG3OE45PjNizszN M3X2OHNCVkeHUh?=
LNCaP-Clone-FGC NF76O5FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1v3UGlEPTB;MUe1Mlg3OSEQvF2= NVfRcZpYW0GQR1XS
YT MlnpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mnr5TWM2OD1zN{[uN|k6KM7:TR?= NU\1NnZ5W0GQR1XS
EW-3 NFTFSJlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX:wZm1qUUN3ME2xO|YvPTV3IN88US=> NYfuXHBoW0GQR1XS
COLO-741 NVyxe2tYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NITVc4ZKSzVyPUG3O{42PjFizszN MoPHV2FPT0WU
SK-NEP-1 NXXmPFdlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1nyTmlEPTB;MUe3MlgxPiEQvF2= M1;VUnNCVkeHUh?=
NB10 NF\mNGNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWnJR|UxRTF5Nz64OFYh|ryP MXHTRW5ITVJ?
SF126 NWS3XppZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYjzR3NZUUN3ME2xO|kvPDN{IN88US=> NVzOdFh{W0GQR1XS
NCI-H522 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1rtcGlEPTB;MUixMlA2QSEQvF2= NGO1WnpUSU6JRWK=
PSN1 NE\0fXBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{L2VWlEPTB;MUixMlY3KM7:TR?= MlWxV2FPT0WU
GI-ME-N NUG3VXB6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlPPTWM2OD1zOEGuOlczKM7:TR?= M133PHNCVkeHUh?=
SBC-5 NYnoOpZsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1O4cGlEPTB;MUixMlk{PCEQvF2= NVvlVYdtW0GQR1XS
ONS-76 Moq0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmDNTWM2OD1zOEKuN|g4KM7:TR?= MoTLV2FPT0WU
LOUCY Ml:5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnW0TWM2OD1zOEKuOlg5KM7:TR?= MWTTRW5ITVJ?
SN12C NXO4[FJHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1POVGlEPTB;MUiyMlgzQSEQvF2= NGLoTVlUSU6JRWK=
A427 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml;KTWM2OD1zOESuOlMyKM7:TR?= NVX1dVRYW0GQR1XS
DOK NGrUcGJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX\JR|UxRTF6ND62OFMh|ryP MXvTRW5ITVJ?
MDA-MB-415 NXO1b4Q6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWHJR|UxRTF6ND62OVEh|ryP M1PsS3NCVkeHUh?=
MRK-nu-1 M1;CTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWDJR|UxRTF6ND65O|Ih|ryP MnPXV2FPT0WU
LB2241-RCC NITMZ3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVLhT5FHUUN3ME2xPFUvOTR3IN88US=> NYrP[nRbW0GQR1XS
NCI-H1563 M3jaXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYrJR|UxRTF6NT6yNFkh|ryP MmnrV2FPT0WU
MDA-MB-453 M4fE[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MY\JR|UxRTF6Nj65OFEh|ryP M3m1c3NCVkeHUh?=
MONO-MAC-6 MmXyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlrRTWM2OD1zOEeuN|kzKM7:TR?= MYfTRW5ITVJ?
CAPAN-1 NWjGfncxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mly4TWM2OD1zOEeuOFU{KM7:TR?= MWXTRW5ITVJ?
SK-LU-1 NX7CSIF2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWLJR|UxRTF6OD60Nlgh|ryP MlTnV2FPT0WU
NCI-H1299 M2rBPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGT0U2NKSzVyPUG4PE44OTlizszN MlvtV2FPT0WU
KG-1 MkLzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFP5bYFKSzVyPUG4PU44QThizszN NVrq[GpRW0GQR1XS
ETK-1 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml\jTWM2OD1zOUCuNVgyKM7:TR?= MV7TRW5ITVJ?
NCI-H838 MkjxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV;JR|UxRTF7MD6zOEDPxE1? NHvlbXNUSU6JRWK=
HCE-4 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHPXWoxKSzVyPUG5NE46QTJizszN Mn71V2FPT0WU
A2780 NI\5d|FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXrJR|UxRTF7Mj64PVEh|ryP M3;VdXNCVkeHUh?=
COLO-668 NFz6XlFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGfEd|NKSzVyPUG5N{4yPTdizszN M3LBdHNCVkeHUh?=
HGC-27 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXPJR|UxRTF7NT61N|gh|ryP NXPtWoI5W0GQR1XS
SW1088 M{LZUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWPGO4F1UUN3ME2xPVUvPjd5IN88US=> NUm4fGpwW0GQR1XS
KOSC-2 MnnYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnHPTWM2OD1zOUWuPFE5KM7:TR?= MYnTRW5ITVJ?
TE-1 M33LSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NETZVGxKSzVyPUG5Ok4zPiEQvF2= NF:w[pZUSU6JRWK=
SW780 Ml[1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml3NTWM2OD1zOU[uO|M1KM7:TR?= M2i5WnNCVkeHUh?=
22RV1 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF34cnNKSzVyPUG5O{41OjRizszN MmOwV2FPT0WU
IA-LM NWTZXHNyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYHJR|UxRTF7Nz64NlIh|ryP M2DmNnNCVkeHUh?=
TE-5 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFG1TlRKSzVyPUG5PE4xOzNizszN M1jl[XNCVkeHUh?=
JVM-3 NVz5eYZHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYnxVGZmUUN3ME2xPVgvPDF7IN88US=> NHjPNJhUSU6JRWK=
HT55 MoXHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NU[yRWlRUUN3ME2xPVkvOTl{IN88US=> MlvMV2FPT0WU
QIMR-WIL MmToS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXL3[45GUUN3ME2yNFAvOTF4IN88US=> M4rjdXNCVkeHUh?=
SJRH30 M4\EUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWDOOZZbUUN3ME2yNFAvPTF3IN88US=> Ml7qV2FPT0WU
COLO-679 M3TPOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2HPOWlEPTB;MkCwMlg2QSEQvF2= NES5dppUSU6JRWK=
MDA-MB-231 NF;Wb2pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYTJR|UxRTJyMD65NFIh|ryP NEfFc|FUSU6JRWK=
MFM-223 NH7wZZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoHvTWM2OD1{MEGuOVg4KM7:TR?= NVjpbZQzW0GQR1XS
MFE-280 M{DFR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mo\1TWM2OD1{MEGuPVYyKM7:TR?= NWfLTHgxW0GQR1XS
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... Click to View More Cell Line Experimental Data

In vivo Oral administration of CHIR-99021 at 30 mg/kg enhances glucose metabolism in a rodent model of type 2 diabetes, with a maximal plasma glucose reduction of nearly 150 mg/dl 3-4 hours after administration, while plasma insulin remains at or below control levels. Oral administration of CHIR-99021 at 16 or 48 mg/kg 1 hour before oral glucose challenges in ZDF rats significantly improves glucose tolerance with 14% and 33% reduction in plasma glucose at 16 mg/kg and 48 mg/kg, respectively, and the higher dose of CHIR-99021 also reduces hyperglycemia before the oral glucose challenge. [1] Administration of CHIR-99021 significantly augments hematopoietic repopulation in recipient mice transplanted with mouse or human hematopoietic stem cells (HSCs), suggesting that GSK-3 is a specific modulator of HSC activity. [4]

Protocol

Kinase Assay:

[1]

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Cell-free kinase assays:

Polypropylene 96-well plates are filled with 300 μL/well buffer (50 mM tris HCl, 10 mM MgCl2, 1 mM EGTA, 1 mM dithiothreitol, 25 mM β-glycerophosphate, 1 mM NaF, 0.01% BSA, pH 7.5) containing 27 nM GSK-3α or 29 nM GSK-3β, and 0.5 μM biotin-CREB peptide substrate. Various concentrations of CHIR-99021 are added in 3.5 μL of DMSO, followed by 50 μL of ATP stock to yield a final concentration of 1 μM ATP in all cell-free assays. After incubation, triplicate 100-μL aliquots are transferred to Combiplate eight plates containing 100 μL/well 50 μM ATP and 20 mM EDTA. After 1 hour, the wells are rinsed five times with PBS, filled with 200 μL of scintillation fluid, sealed, left 30 minutes, and counted in a scintillation counter. All steps are performed at room temperature.
Cell Research:

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  • Cell lines: INS-1E
  • Concentrations: Dissolved in DMSO, final concentrations ~ 20 μM
  • Incubation Time: 1, or 4 days
  • Method:

    Cells are maintained for 24 hours in starvation medium (culture medium with only 5 mM glucose, 1% fetal calf serum). Then cells are exposed to various concentrations of CHIR-99021 for 1, or 4 days. Cell number is measured by staining of cellular DNA with CyQuant dye, which becomes fluorescent when bound to DNA. Fluorescence is measured after 30 minutes of incubation using the FLUOstar Optima reader. Cell replication is determined by BrdUrd incorporation. BrdUrd labeling solution is added to the medium for the last 4 hours before the cells are fixed using FixDenat solution and incubated with monoclonal anti-BrdUrd-POD antibodies. After substrate solution is added to each well, the light emission is measured in a microplate luminometer using the Analyst HT detection system.


    (Only for Reference)
Animal Research:

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  • Animal Models: Female db/db mice or male ZDF rats with type 2 diabetes
  • Formulation: Formulated as solutions in 20 mM citrate-buffered 15% Captisol or as fine suspensions in 0.5% carboxymethylcellulose.
  • Dosages: ~48 mg/kg
  • Administration: Orally
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 93 mg/mL (185.33 mM)
Ethanol 2 mg/mL (3.98 mM)
Water Insoluble
In vivo Add solvents individually and in order:
5% DMSO+30% PEG 300+ddH2O
24mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 501.8
Formula

C22H18Cl2N8.HCl

CAS No. 1797989-42-4
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
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    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    I recently purchased CHIR 99021 to inject mice intraperitoneally. The data sheet says using 10% captisol for in vivo use. Is it for IP injection? Or do you have other solutions to suggest to use for in vivo IP injection of CHIR 99021?

  • Answer:

    The formulation of 15% Captisol is for oral gavage, and S2924 CHIR-99021 (CT99021) HCl in it is a suspension. S2924 CHIR-99021 (CT99021) HCl can be dissolved in 5% DMSO+30% PEG 300+ddH2O at 24mg/ml as a clear solution for IP injection.

  • Question 2:

    How about the half-life of this compound? If it is necessary for daily treatment when the compound is used in long-term experiment?

  • Answer:

    Please change the culture medium each day to add the new comound in it if the duration is more than 72h.

  • Question 3:

    What is the difference between S2924 and S1263? Which one is better for differentiation?

  • Answer:

    S1263 is the free base format of Chir-99021, while S2924 is the HCl salt format. They have the same biological activity but are slightly different in solubility.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID