CHIR-99021 (CT99021) HCl

Catalog No.S2924

CHIR-99021 (CT99021) HCl Chemical Structure

Molecular Weight(MW): 501.8

CHIR-99021 (CT99021) HCl is hydrochloride of CHIR-99021, which is a GSK-3α/β inhibitor with IC50 of 10 nM/6.7 nM in cell-free assays; ability to distinguish between GSK-3 and its closest homologs Cdc2 and ERK2.

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In DMSO USD 340 In stock
USD 90 In stock
USD 170 In stock
USD 570 In stock

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Cited by 66 Publications

7 Customer Reviews

  • Differentiation induced by treatment with the Gsk3 inhibitor in hPSCs is β-catenin dependent. H9-7TGP cells were treated with 12 uM CH in mTeSR1 for 4 d. Immunofluorescent staining for Oct4, Isl1, and Nkx2.5 was compared with GFP expression. (Scale bars, 50 uM.)

    Proc Natl Acad Sci U S A 2012 109(27):E1848-57. CHIR-99021 (CT99021) HCl purchased from Selleck.

    Gpc4 regulates ESC response to Wnt/β-catenin signaling.  (E) Quantitative analysis of control and Gpc4-mutant beating foci exposed to the GSK3 inhibitor, CHIR 99021 (1 and 3 μM, from day 0 to day 5). Analysis was done at different time points. Gpc4- mutant cells treated with CHIR 99021 behave as controls (mean±SEM; n=2).

    Stem Cells 2012 30, 1863-1874. CHIR-99021 (CT99021) HCl purchased from Selleck.

  • Inhibition of GSK3 activity induces an autophagic response in human pancreatic cancer cells. PANC1 cells were treated for 24 h with DMSO, CHIR99021 (CHIR; 5 uM) or the mTOR inhibitor Torin1 (250 nM). Autophagosome (punctate LC3B) (D) and lysosome (E) labeling was visualized with a Zeiss LSM700 confocal microscope. Images were acquired with a 20x (D) or 63x (E) objective. Nuclei were stained with DAPI. Scale bars, 20 祄.

    J Biol Chem 2015 290(9), 5592-605 . CHIR-99021 (CT99021) HCl purchased from Selleck.

     

    Characterization of rES cells. A: image of normal rES cell colonies on feeder layers. B: rES colonies were positive for AP staining. CeE: rES colonies readily expressed pluripotent markers, Sox2 (C), Oct4 (D), and SSEA-1 (E). Blue, DAPI. Scale bars: 100 um.

    J Genet Genomics 2012 39, 643e651. CHIR-99021 (CT99021) HCl purchased from Selleck.

  •  

    Smad linker phosphorylation involves GSK3 in human melanoma lines. Inhibition of Smad2 and Smad3 linker phosphorylation in the presence of the specific GSK3 specific inhibitor, CT99021. 24 hours post seeding, WM793 and 1205LU cells were serum-starved for about 16 hours, and incubated in the absence (2) or presence (+) of 2 uM of CT99021 for two hours. Immunoblots were performed as in A. p: Phosphorylated. S: Serine; T: Threonine.

    PLoS One 2012 7, e47312. CHIR-99021 (CT99021) HCl purchased from Selleck.

     

    PANC1 cells were treated with GSK3 inhibitors SB216763 (SB 10µM or SB 20µM) or CHIR99021 (CHIR 5µM) for 72h.

    CHIR-99021 (CT99021) HCl purchased from Selleck.

  •  

     Activity of canonical WNT/ β -CATENIN signals induced by CHIR 99021 in the developing human lung. Human lung tissue explants obtained at 15 weeks of gestation were exposed to 0, 5 and 10 μM CHIR 99021 in vitro for 72 h. (A-B) Western blot analysis showed expression of β -CATENIN in human lung tissues. Results are presented as the mean ± sem of three independent experiments. *P < 0.05, **P < 0.01. (C) qRT-PCR analysis detected a dose-dependent increase in WNT signaling transcription factors and target genes following exposure to CHIR 99021. Results are presented as the mean ± sem of three independent experiments. *P < 0.05, **P < 0.01. (D-K) Histology examination showed obvious morphogenetic changes of the airway epithelial cells associated with CHIR 99021 treated lung tubes. Scale bars: D-G =100 μm; H-K = 20 μm.

    CHIR-99021 (CT99021) HCl purchased from Selleck.

Purity & Quality Control

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Notes:

2. For more details, such as half maximal inhibitory concentrations (IC50s) and working concentrations of each inhibitor, please click on the link of the inhibitor of interest.
3. "+" indicates inhibitory effect. Increased inhibition is marked by a higher "+" designation.
4. Orange "√" refers to compounds which do inhibitory effects on the related isoform, but without specific value.

Biological Activity

Description CHIR-99021 (CT99021) HCl is hydrochloride of CHIR-99021, which is a GSK-3α/β inhibitor with IC50 of 10 nM/6.7 nM in cell-free assays; ability to distinguish between GSK-3 and its closest homologs Cdc2 and ERK2.
Targets
GSK-3β [1]
(Cell-free assay)
GSK-3α [1]
(Cell-free assay)
Tie-2 [1]
(Cell-free assay)
FLT1 [1]
(Cell-free assay)
KDR [1]
(Cell-free assay)
6.7 nM 10 nM >5 μM >5 μM >5 μM
In vitro

CHIR-99021 shows greater than 500-fold selectivity for GSK-3 versus its closest homologs CDC2 and ERK2, as well as other protein kinases. Furthermore, CHIR-99021 shows only weak binding to a panel of 22 pharmacologically relevant receptors and little inhibitory activity against a panel of 23 nonkinase enzymes. CHIR-99021 induces the activation of glycogen synthase (GS) in insulin receptor-expressing CHO-IR cells with EC50 of 0.763 μM. [1] In addition to simulating the actions of insulin, inhibition of GSK-3 by CHIR-99021 (3 μM) increases free cytosolic β-catenin by 1.9-fold, mimicking the canonical Wnt signaling pathway in 3T3-L1 preadipocytes. During any of the first 3 days of differentiation, CHIR-99021 treatment inhibits the preadipocyte differentiation with IC50 of 0.3 μM by blocking induction of CCAAT/enhancer-binding protein α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ). [2] Unlike lithium chloride and AR-A014418, CHIR-99021 treatment does not reduce the viability of INS-1E cells even at high concentrations. Instead, CHIR-99021 robustly increases the rate of proliferation of INS-1E cells in a dose-dependent manner, and significantly inhibits INS-E cell death induced by high glucose and high palmitate in a concentration-dependent manner. CHIR-99021 promotes primary beta cell replication in isolated rat islets at concentrations as low as 1 μM, with 2-3 fold increase of cell replication at 5 μM of CHIR-99021 treatment. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
3T3-L1 M3HOfGZ2dmO2aX;uJGF{e2G7 M3LpVlMh|ryP M1P2OFQhcA>? NUjIPI5HTE2VTx?= MVXJcohq[mm2czDwdoVi\Gmyb3P5eIUh\GmoZnXy[Y51cWG2aX;u MVexNlA2PTJyMB?=
insulin receptor–expressing CHO-IR M1i1OmZ2dmO2aX;uJGF{e2G7 NWfkXWhuPTByIH7N NWqxUZk6OzBibXnu M1i4V2ROW09? MoS1RYN1cX[jdHXzJGdtgWOxZ3XuJHN6dnSqYYPlJEhIWyl? MVyxNlYxPjR7Nx?=
H4IIE NVvEUI91TnWwY4Tpc44hSXO|YYm= NVLBNWVKOTBizszN MnrsNlQhcA>? M{\qUGROW09? NE\sU3dT\We3bHH0[ZMh|rJvY3H0[Y5qdiCjY4Tpeol1gSCjbnSgWGlTTSClb370ZYlvcW6pIIDyc41wfGW{IHHjeIl3cXS7 MYOxOVM2ODF7NR?=
Oct4/Sox2/Klf4-infected MEFs NXvmN45UTnWwY4Tpc44hSXO|YYm= MUixNEDPxE1? NGKzcoUzQCCm MnzUSG1UVw>? Mo[3VJJwdW:2ZYOgUWVHeyC{ZYDyc4dz[W2vaX7nJHRz[W6|ZIXj[YQh[nliT3P0OEwhW2:6MjygZY5lKEuuZkS= MYmxPVg{QTB3NR?=
Sca-1+ HPC MonTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV3vS2RmOiEQvF2= MmLaOkBl MUfEUXNQ NEnGWnJUgW6ncnfpd5Rq[2GubImgdJJwdW:2ZYOgV4NiNTFtwrDIVGMheHKxbHnm[ZJifGmxbjDhcoQh[2:ub375JIZwem2jdHnvckB1d2endHjldkB4cXSqIFXHSi=> MU[yNVYzPDN2OB?=
HMC1.2 Mn\rS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NITtbFQ{OCEQvF2= NF\VeGg4OiCq MmHjSG1UVw>? MXfS[YR2[2W|IHPlcIwhe3W{dnn2ZYw> NUX3[3lnOjJyM{mzNFE>
Endothelial cell(EC) MX3GeY5kfGmxbjDBd5NigQ>? MUmzJO69VQ>? NGfzdI1FVVOR NIjZOnhRem:vb4Tld{BmdmSxdHjlcIlidCClZXzsJEhGSyliZHnm[oVz\W62aXH0bY9v MmWyNlIxPTJyME[=
PANC1 MkfjRZBweHSxc3nzJGF{e2G7 MlT5xsA2KM7:TR?= Mo\aO|IhcA>? NXvPe|JsTE2VTx?= NF\LRnZVemmpZ3Xyd{BidiCjcH;weI91cWNicnXzdI9ve2V? MoTlNlIzODFzOE[=
INS-1E NF7ISZVHfW6ldHnvckBCe3OjeR?= NUfRcmdCOi53IN88US=> MXWyOEBp NYH4bFdmTE2VTx?= NXnJOFE5UW6mdXPld{BiKHKnZIXjeIlwdiCxZjDOVFEhd3[ncnX4dJJme3Orb36= NVK5OpRTOjJ2Mke3NFQ>
hiPSC-NPs MmPuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYWz5qCK|ryP M{fQblczKGh? NIPp[JdFVVOR NWnyZYlzUW6lcnXhd4V{KGOnbHygdJJwdGmoZYLheIlwdsLi NHH2NoIzOjZ2Mk[4Oy=>
platelets NHXlRlBHfW6ldHnvckBCe3OjeR?= MWOxNEDPxE1? M1O0SlE2KG2rbh?= M1f3[GROW09? MoPrTY5kemWjc3XzJJRpem:vYnnuMY1m\GmjdHXkJJBt[XSnbHX0JIFo\3KnZ3H0bY9vKGGwZDDBWHAhe2WlcnX0bY9v NUHKZopHOjN{M{m4O|c>
DN3 MUDGeY5kfGmxbjDBd5NigQ>? NFLXZXYyOCEQvF2= M2q4clk3KGh? NFTXXWNFVVOR NFr4XJdKdmO{ZXHz[ZMh[2WubDDk[ZZmdG:ybXXueEBidmRicILvcIln\XKjdHnvci=> MmXnNlM2OjZ7OEm=
embryonic stem cells (ESC) M3zwfWZ2dmO2aX;uJGF{e2G7 M4jDbFMhyrWP M3XxSFEyKGR? MkjHSG1UVw>? MXzQdo9ud3SnczDz[YxnNXKnbnX3ZYwhd2ZibnJDs5ZmNXS7cHWgcY92e2ViZX3idplwdmmlIIP0[Y0h[2WubIOgLGVUSyl? NUK4em5vOjN6NEOzNVI>
H460 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUe2foFQPSEEtV2= NHfwTmQzPCCq M{K2R2ROW09? NXm5d3hmWHKxbX;0[ZMh[2WubDDwdo9tcW[ncnH0bY9v M4jqNVI1OzZ5Nki4
H157 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYHRVItxPSEEtV2= NHTB[pAzPCCq NFPUfVBFVVOR MXXQdo9ud3SnczDj[YxtKHC{b3zp[oVz[XSrb36= M13Mb|I1OzZ5Nki4
H460 MYTBdI9xfG:|aYOgRZN{[Xl? Mm\JOUDDvU1? NUDmUldnOjRiaB?= MnXQSG1UVw>? MWHBeJRmdnWjdHXzJINmdGxiYYDvdJRwe2m| MW[yOFM3PzZ6OB?=
H157 Mn;WRZBweHSxc3nzJGF{e2G7 M{Lhd|UhyrWP MljsNlQhcA>? NYjVe4tJTE2VTx?= Mnj5SI9meyCwb4Sgd4lodmmoaXPhcpRtgSCjZn\lZ5Qh[XCxcITvd4l{ M3z4dVI1OzZ5Nki4
A549 MojMRZBweHSxc3nzJGF{e2G7 M2HucVUhyrWP NUTGVJFDOjRiaB?= M{K0UmROW09? MY\JcoNz\WG|ZYOgZ4VtdCCjcH;weI9{cXN? NUi1O4p4OjR|Nke2PFg>
Neural progenitor cells NVW3[HduTnWwY4Tpc44hSXO|YYm= MojwN{DPxE1? M3PFT|ExKGR? MX7EUXNQ NXm1XVFEUW6mdXPld{Bo\W6ncnH0bY9vKG:oIH7leZJidCCycn;n[Y5qfG:{IHPlcIx{ MXmyOFY{QDB|NB?=
ES-D3 NGPCU3REgXSxdH;4bYMhSXO|YYm= MX:xNOKh|ryP NHO2WXA{KGR? NVPUcohLTE2VTx?= MYjJcoR2[2W|IH3v[IVz[XSnIHP5eI91d3irY3n0fUB4cXSqIFnDOVAhd2ZiND65JO69VQ>? MW[yOFc4QTN4NR?=
ES-CCE NGK1[mtEgXSxdH;4bYMhSXO|YYm= NYPzTldEOTEEoN88US=> M3;sTVMh\A>? M3vKfmROW09? NXr3dZIzUW6mdXPld{Bud2SncnH0[UBkgXSxdH;4bYNqfHl? MlfmNlQ4Pzl|NkW=
ES-D3 NXjUcnVWTnWwY4Tpc44hSXO|YYm= MnPnOeKh|ryP MXKzJIQ> NVnkXG9yTE2VTx?= MWLTeJJwdmeueTDpcoR2[2W|IITo[UBi[3Srdnn0fUBw\iCZboSvZoV1[S2lYYTlcolvKHCjdHj3ZZk> MX2yOFc4QTN4NR?=
ES-CCE NVnaS49pTnWwY4Tpc44hSXO|YYm= MWW1xsDPxE1? Ml\QN{Bl NGq2R|NFVVOR MXnTeJJwdmeueTDpcoR2[2W|IITo[UBi[3Srdnn0fUBw\iCZboSvZoV1[S2lYYTlcolvKHCjdHj3ZZk> M2nSO|I1Pzd7M{[1
SW982 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3v5eGlEPTB;MD6yNVQyPiEQvF2= M3PESnNCVkeHUh?=
GCT MlvrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVq4WJNiUUN3ME2wMlMxPjd{IN88US=> MYfTRW5ITVJ?
769-P NF;DVIZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYLJWXZ5UUN3ME2wMlU5PDl7IN88US=> MnvhV2FPT0WU
LOXIMVI M3XPZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWXJR|UxRTBwNUi4N|Ih|ryP NUDYVIJJW0GQR1XS
D-336MG MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYnJR|UxRTBwOES2NkDPxE1? NGPjOG5USU6JRWK=
S-117 MnHHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1rrO2lEPTB;MD65NlE{OSEQvF2= MXvTRW5ITVJ?
D-247MG NFWwfGVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUjJR|UxRTBwOUS4OVIh|ryP NYXodGlJW0GQR1XS
TYK-nu MnjSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnLvTWM2OD1zLkC3PVEzKM7:TR?= NHTZRmdUSU6JRWK=
A498 NX;abXVoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUjKUmZ4UUN3ME2xMlMxPDZ7IN88US=> NVTDW3JZW0GQR1XS
BCPAP M13CS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHrNOYxKSzVyPUGuOlY5QThizszN NIHZUVdUSU6JRWK=
KYSE-180 Mnj4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NInoU2ZKSzVyPUGuO|I4QDhizszN NX[5R3VJW0GQR1XS
MIA-PaCa-2 MlvES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn\0TWM2OD1zLke1PVc4KM7:TR?= MknjV2FPT0WU
HCC1395 M1HHcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXrnbVduUUN3ME2xMlc5Ojd|IN88US=> NIP4fnJUSU6JRWK=
RS4-11 M1m2NWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NY\iPVNwUUN3ME2xMlk2PDF3IN88US=> M3XNeXNCVkeHUh?=
NCI-H2122 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2HmSWlEPTB;Mj6yO|Y5PCEQvF2= NWHiZmM1W0GQR1XS
SNU-423 NGnw[VNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmnGTWM2OD1{LkS5O|g4KM7:TR?= NIew[WNUSU6JRWK=
SK-LMS-1 NHrJNVlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF\2NmNKSzVyPUKuOlQyOjhizszN NH3PbGdUSU6JRWK=
RPMI-7951 M4OwPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3zWXGlEPTB;Mj63NFE6OyEQvF2= NV7tboxpW0GQR1XS
COLO-829 M{C1fmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFrEWYJKSzVyPUKuO|Y2PCEQvF2= M2\PVnNCVkeHUh?=
SCC-9 MljjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHPnVJVKSzVyPUKuPVU1QDVizszN MVvTRW5ITVJ?
A704 NWrpe5MxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3nxc2lEPTB;Mz6wPFEzKM7:TR?= M37OUnNCVkeHUh?=
HOP-62 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M133cmlEPTB;Mz6zOlA2PCEQvF2= NEX4e|hUSU6JRWK=
HCC1569 M2W5S2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGPj[YtKSzVyPUOuOFg{ODVizszN MYLTRW5ITVJ?
A172 MnjrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkiwTWM2OD1|Lke1OFk5KM7:TR?= M1PHS3NCVkeHUh?=
MOLT-16 M2n5UGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWHJR|UxRTNwOEi1OVYh|ryP NWHqeGNKW0GQR1XS
TE-15 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmDJTWM2OD1|Lkm1N|Q{KM7:TR?= M2XEZXNCVkeHUh?=
OE19 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlXQTWM2OD1|Lkm2OFUyKM7:TR?= MmLSV2FPT0WU
MHH-ES-1 NFTRW41Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2HTRmlEPTB;ND6wOFU1PSEQvF2= NIjzTW9USU6JRWK=
NKM-1 MnjVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXW5RXJPUUN3ME20MlE{QTdzIN88US=> MVfTRW5ITVJ?
RCC10RGB MkHWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGPZcpFKSzVyPUSuNlk1PzlizszN NWDaS|NzW0GQR1XS
BxPC-3 MoPvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVjFeplUUUN3ME20MlcxOzlzIN88US=> M{TkPHNCVkeHUh?=
ALL-PO M2TvbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnLVTWM2OD12LkizPFU5KM7:TR?= NHu0bFNUSU6JRWK=
SK-OV-3 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUfGbm1TUUN3ME20Mlk6OzRizszN M1rQbnNCVkeHUh?=
SW1710 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHfld4dKSzVyPUWuOFI1PTZizszN NFv6NGxUSU6JRWK=
NCI-H2452 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnPQTWM2OD13LkS1OlI5KM7:TR?= NV;XU2Z[W0GQR1XS
D-392MG M2\NT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYTPdIlkUUN3ME21MlQ6OTR3IN88US=> MULTRW5ITVJ?
LB2518-MEL MmjkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFrLR2FKSzVyPUWuO|I3OjdizszN NYTLXVJlW0GQR1XS
BL-41 Mln6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEnDbGhKSzVyPUWuPFEzPjZizszN M{f3SXNCVkeHUh?=
WM-115 MnXPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnK0TWM2OD13LkixOVA6KM7:TR?= NH\hPINUSU6JRWK=
SK-MEL-2 NXrKSnZbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGLzWJBKSzVyPU[uN|g{PDRizszN NX7LW4Q1W0GQR1XS
CGTH-W-1 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{Pm[mlEPTB;Nj60NVch|ryP MWHTRW5ITVJ?
BB65-RCC MnHYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlfRTWM2OD14LkWzNVk2KM7:TR?= M4HTPXNCVkeHUh?=
CAL-85-1 NFT4TWNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX7JR|UxRTZwNkC5N|Yh|ryP NIXVSW1USU6JRWK=
RT-112 NGrod4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnHTTWM2OD15LkG2OFU3KM7:TR?= M3f6fXNCVkeHUh?=
MC116 NY[3dHNRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3fQSWlEPTB;Nz6yPFY6OSEQvF2= MmC0V2FPT0WU
G-361 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3\lRmlEPTB;Nz60OlU3OSEQvF2= NYTLT|c5W0GQR1XS
SW1417 NYnhZXJPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVvJR|UxRTdwNkS1PFQh|ryP M2np[nNCVkeHUh?=
MV-4-11 MmH0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV\1TnZXUUN3ME24MlU1OzB2IN88US=> M1W0fHNCVkeHUh?=
CAL-72 NVPrO25mT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M13SV2lEPTB;OD63OlEyOyEQvF2= NUjrNpozW0GQR1XS
MKN28 NEni[21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHvJPYxKSzVyPUiuPFg2OTZizszN MkjIV2FPT0WU
MEG-01 M3e0bmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1nZ[mlEPTB;OD65PVA1OyEQvF2= NEXHeFZUSU6JRWK=
ACHN NHjRWIFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M37nV2lEPTB;OT6zNFU1PiEQvF2= MmHMV2FPT0WU
NCI-H1755 M3zyeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXTJR|UxRTlwM{m5PFYh|ryP MkHYV2FPT0WU
NCI-H64 M1rtWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmLrTWM2OD17LkSyNlU2KM7:TR?= MlO4V2FPT0WU
COLO-205 Mln0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWLHN21QUUN3ME25MlQ{QDJzIN88US=> NXjSNVlbW0GQR1XS
CTV-1 NG\xWVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVjJR|UxRTlwNEO4PVQh|ryP NHHIS2NUSU6JRWK=
U-2-OS NYTmdXpMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MULJR|UxRTlwNEWwNFYh|ryP Mlm4V2FPT0WU
LB996-RCC NIW4OpJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{jOXmlEPTB;OT61NlM6PyEQvF2= M{f0OnNCVkeHUh?=
SK-N-DZ NIDUe25Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXLJR|UxRTlwNkW3OFIh|ryP MmfGV2FPT0WU
EFM-19 NHrzWG1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUPJR|UxRTlwN{W1OlYh|ryP Mnf1V2FPT0WU
DBTRG-05MG MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3HLVGlEPTB;OT63O|c4KM7:TR?= NVvjWo9GW0GQR1XS
U-118-MG MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn;JTWM2OD17Lkm0OFQ1KM7:TR?= NEPsVnFUSU6JRWK=
LB1047-RCC NFv1OlVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF;a[XVKSzVyPUmuPVk4QThizszN M3rIRnNCVkeHUh?=
NCI-H28 M{\SbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnS0TWM2OD1zMD60OFE{KM7:TR?= MY\TRW5ITVJ?
MZ2-MEL MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXjJR|UxRTFyLkS2O|ch|ryP M4DOZnNCVkeHUh?=
M059J NF3nU3FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml\PTWM2OD1zMD62NVAzKM7:TR?= MmDpV2FPT0WU
HT-3 NIPZZ2JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NETjeGhKSzVyPUGwMlYyPzlizszN M{f5WnNCVkeHUh?=
SNU-387 NHu1VWNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnHETWM2OD1zMD63NlM2KM7:TR?= NVriWY5OW0GQR1XS
NCI-H526 NW\UdHV7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoHkTWM2OD1zMT6xN|Y2KM7:TR?= M2\lUHNCVkeHUh?=
HEL NU\L[pdWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIPqTnVKSzVyPUGxMlY2PzlizszN M2fZO3NCVkeHUh?=
PA-1 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYPJR|UxRTFzLkmzJO69VQ>? MYXTRW5ITVJ?
PC-14 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYrJR|UxRTF{LkOyOEDPxE1? NEDySoNUSU6JRWK=
C8166 NIHmc3BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmrhTWM2OD1zMz6yN|E5KM7:TR?= Mon4V2FPT0WU
NCI-H82 NVT0eFRbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoDhTWM2OD1zMz6yPVA6KM7:TR?= M2DqXHNCVkeHUh?=
DSH1 Mo\rS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoTaTWM2OD1zMz6zPFc6KM7:TR?= MX3TRW5ITVJ?
T98G NFfhNpJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVPJR|UxRTF|LkS3NVch|ryP M{j2S3NCVkeHUh?=
KU812 MonNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGG0[|JKSzVyPUGzMlUyPzVizszN NWXvfVY3W0GQR1XS
SW48 MlXiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4\Y[GlEPTB;MUOuO|I3PSEQvF2= NWTV[XZLW0GQR1XS
MEL-HO NHHuVIRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEj5OlJKSzVyPUGzMlg5OjlizszN MVfTRW5ITVJ?
SF539 M{HBRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHXnVIxKSzVyPUG0MlAyOTZizszN MYPTRW5ITVJ?
NB1 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn7BTWM2OD1zND6xPVM2KM7:TR?= MmLsV2FPT0WU
ST486 NHTpR4NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NULNclJLUUN3ME2xOE4zOjVzIN88US=> M2LvSnNCVkeHUh?=
LoVo MlPLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYrJR|UxRTF3LkK2OVkh|ryP NX\VOJU5W0GQR1XS
NH-12 MoDxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFy3XHdKSzVyPUG2MlAxQTdizszN NYHzV5V3W0GQR1XS
CCRF-CEM MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2HNTWlEPTB;MU[uOlcxOiEQvF2= NEPl[ZJUSU6JRWK=
Daudi NIXBWFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mo[0TWM2OD1zNz62NVQ6KM7:TR?= NEXJ[21USU6JRWK=
SIMA MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF7xV4FKSzVyPUG3MlkyOThizszN NUjjRopzW0GQR1XS
8305C NGLkXWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV;ubYw4UUN3ME2xPE42OzB3IN88US=> NHn6NWlUSU6JRWK=
EM-2 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYC5dmlHUUN3ME2xPE43OjR6IN88US=> MkjsV2FPT0WU
SNU-449 M1ezZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX;rc2M6UUN3ME2xPE44OTVizszN MlOwV2FPT0WU
A101D M3fvO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1TnZmlEPTB;MUmuNlQ{KM7:TR?= NVTwTXVlW0GQR1XS
MHH-NB-11 NWHKfZgyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVrGe3RiUUN3ME2xPU42OzR{IN88US=> M36xRXNCVkeHUh?=
Becker NHjNR5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MojjTWM2OD1{MD6xO|c4KM7:TR?= M{W0SHNCVkeHUh?=
NCI-H727 M4\RUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIrlbZVKSzVyPUKwMlIyPjlizszN NHfLRWpUSU6JRWK=
HCC1187 M4njW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIO0SWtKSzVyPUKwMlM2QDlizszN M4OxOHNCVkeHUh?=
KYSE-450 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWfJR|UxRTJzLkWzOVQh|ryP MX3TRW5ITVJ?
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BPH-1 NVPTNW9yT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHn5VXlKSzVyPUKxMlcxOzVizszN Mn\HV2FPT0WU
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TGBC24TKB M1;yOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVfFb3VNUUN3ME2yNU45QTV5IN88US=> MnS3V2FPT0WU
KARPAS-299 NXT1[IdET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmPwTWM2OD1{Mj6xPVU1KM7:TR?= M{nRXHNCVkeHUh?=
SW684 Mk\sS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYDJR|UxRTJ{LkK4OVgh|ryP NYPIRnVPW0GQR1XS
RXF393 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHrtfGpKSzVyPUKyMlYxPjhizszN M{LycnNCVkeHUh?=
LS-513 NGXkcpRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV7TN5d3UUN3ME2yNk43OTl{IN88US=> Mn7VV2FPT0WU
KURAMOCHI NEKyVVVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYfib3NRUUN3ME2yNk44ODZ7IN88US=> M{j6eHNCVkeHUh?=
GI-1 MkP4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYXnbW1sUUN3ME2yNk44QDRzIN88US=> Mo\0V2FPT0WU
M14 NXXDc3kxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MojJTWM2OD1{Mz6xNVMzKM7:TR?= NUn1WlBuW0GQR1XS
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PANC-10-05 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1HnTmlEPTB;MkSuNFg6QCEQvF2= MX7TRW5ITVJ?
HMV-II MlvXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mnr5TWM2OD1{ND6yNVY{KM7:TR?= NEXJPI1USU6JRWK=
NCI-H1963 NViyRZlXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYLQ[nRMUUN3ME2yOE4zQDN7IN88US=> MYrTRW5ITVJ?
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NCI-H1770 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MU\JR|UxRTJ4Lki5PFYh|ryP MX;TRW5ITVJ?
SNG-M MnzhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWTJR|UxRTJ5LkKyO|Eh|ryP MXTTRW5ITVJ?
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NCI-H2170 MonMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV[4bJJXUUN3ME2yO{42Ojh{IN88US=> NH2zbGVUSU6JRWK=
COLO-792 NVXuPYxqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1LQWGlEPTB;MkeuO|czQCEQvF2= NF;wc4lUSU6JRWK=
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MC-CAR Ml\6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3flVGlEPTB;MkeuO|kxPyEQvF2= MYDTRW5ITVJ?
SU-DHL-1 NV7YcmF3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEjoPWZKSzVyPUK3Mlk1PzRizszN NHLnWWJUSU6JRWK=
OAW-28 NXP5S3RUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXPIOXB4UUN3ME2yPE4xODV{IN88US=> NH\qZWtUSU6JRWK=
C32 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlyyTWM2OD1{OD6wPFI3KM7:TR?= M3PvcHNCVkeHUh?=
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JiyoyeP-2003 Mn7ZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mnf3TWM2OD1{OT62O|E6KM7:TR?= M4LpXnNCVkeHUh?=
A204 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnjPTWM2OD1{OT62PFI5KM7:TR?= M4TZZ3NCVkeHUh?=
IST-MES1 Mly2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVTJR|UxRTJ7LkewPVIh|ryP MoHaV2FPT0WU
LCLC-103H NIfNZ21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mk\xTWM2OD1|MD6yNFU6KM7:TR?= MX7TRW5ITVJ?
CAKI-1 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGjGdYRKSzVyPUOwMlI3PTZizszN Mln0V2FPT0WU
NALM-6 NYT0TlNxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYThcJhbUUN3ME2zNE41QTZ3IN88US=> MoLnV2FPT0WU
SK-MM-2 MljRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXXJR|UxRTNyLkm3PVIh|ryP MWTTRW5ITVJ?
LB373-MEL-D MkHwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmTOTWM2OD1|MT6wNFQ4KM7:TR?= MkjmV2FPT0WU
CAS-1 NFX3R2ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1TyeWlEPTB;M{GuO|Y3PSEQvF2= M1rVTnNCVkeHUh?=
KNS-42 NHLFOVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MU\JR|UxRTNzLki2OlIh|ryP NXq2RoRVW0GQR1XS
SW948 NEWwcWVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVnJR|UxRTN{Lke1OFgh|ryP NIfKRZNUSU6JRWK=
HCC1143 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX\JR|UxRTN{Lke5O|kh|ryP M{ToNHNCVkeHUh?=
NCI-H1648 NFfr[IdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml3DTWM2OD1|Mj64OFY4KM7:TR?= MWLTRW5ITVJ?
RVH-421 NGmwcnZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXXJR|UxRTN|LkG2PEDPxE1? NHPVV4NUSU6JRWK=
KU-19-19 NEnPPXlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX\JR|UxRTN|Lke1N|Qh|ryP NF7kfVlUSU6JRWK=
PFSK-1 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYG2eZZlUUN3ME2zOE46OjRzIN88US=> Mo\MV2FPT0WU
TK10 Ml7xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX\JTIVbUUN3ME2zOU4yOzJ{IN88US=> M{jrUHNCVkeHUh?=
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HT-144 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{fpVGlEPTB;M{iuNlU4PSEQvF2= NGHJVlNUSU6JRWK=
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KYSE-270 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{GxRmlEPTB;NEWuNFQ{PyEQvF2= MWPTRW5ITVJ?
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LS-123 Mk\GS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWHzcoNsUUN3ME2zNlgvOTZ2IN88US=> M2DZOXNCVkeHUh?=
SF268 NUTOVGk2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkjyTWM2OD1|M{CuNlY2KM7:TR?= M4LNSXNCVkeHUh?=
BT-474 NYnEO492T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYryWoFNUUN3ME2zN|IvPDF6IN88US=> NWDpdmZ[W0GQR1XS
NCI-H1522 NInoRlhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHXsPGVKSzVyPUOzOE4{OzlizszN NWrlVXlQW0GQR1XS
LC-2-ad NHzUOYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWDJR|UxRTN|Nj61PVEh|ryP MmjDV2FPT0WU
LAN-6 NWDvd5pRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX;JR|UxRTN|Nj65O|ch|ryP NFXwRlJUSU6JRWK=
ARH-77 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX;JR|UxRTN|Nz6zNkDPxE1? MW\TRW5ITVJ?
HuO9 MnroS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFvzR2ZKSzVyPUOzO{41PyEQvF2= NXvHXpNWW0GQR1XS
MLMA Mlf3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnHQTWM2OD1|M{iuNVU2KM7:TR?= M3nWT3NCVkeHUh?=
Raji MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWXJR|UxRTN|OD6zPEDPxE1? NHn4N4JUSU6JRWK=
GT3TKB M2\QTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXXTPXZrUUN3ME2zN|gvPDF{IN88US=> NUfIXmdHW0GQR1XS
NCI-H441 NX3OTGlkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXjjVldXUUN3ME2zOFEvPDF6IN88US=> MnvnV2FPT0WU
NCI-H2030 MnjFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkLuTWM2OD1|NEGuOlA6KM7:TR?= MlTHV2FPT0WU
VM-CUB-1 M1PhWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGntZolKSzVyPUO0Nk43OzZizszN MWPTRW5ITVJ?
KP-4 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWjhSHJlUUN3ME2zOFMvPzN|IN88US=> NIn6PIZUSU6JRWK=
PF-382 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYfJR|UxRTN2ND60NlIh|ryP NVTzV5JMW0GQR1XS
NB13 NF;RT2xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkXlTWM2OD1|NEWuOVgh|ryP NUjxe|AxW0GQR1XS
D-502MG M1vlVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX\JR|UxRTN2NT64NVIh|ryP M2\3UXNCVkeHUh?=
CAL-54 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml2wTWM2OD1|NEWuPFc6KM7:TR?= Ml3sV2FPT0WU
DOHH-2 NF[yRVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYfsbXJPUUN3ME2zOFYvPjVizszN NWm0dohNW0GQR1XS
SK-PN-DW MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M331V2lEPTB;M{S3MlQzPiEQvF2= MkHzV2FPT0WU
NCI-H720 NHrXd4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVXJR|UxRTN2OT64OFYh|ryP M3XXdHNCVkeHUh?=
SW1990 MmXiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWfMXoxrUUN3ME2zOVEvOjhizszN MmXGV2FPT0WU
NCI-H2196 NV7Qb5VUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn3ETWM2OD1|NUKuOlI1KM7:TR?= NELPXmVUSU6JRWK=
P30-OHK NUP2cWJXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MonkTWM2OD1|NUOuNFY{KM7:TR?= M{D2e3NCVkeHUh?=
NCI-H358 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1zMbWlEPTB;M{W1MlM2PiEQvF2= NVHmb2doW0GQR1XS
CW-2 MnLJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXnEdYgyUUN3ME2zOVYvPTJ7IN88US=> MmqzV2FPT0WU
COLO-678 NU\GTmRHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYjJR|UxRTN3Nz64OlMh|ryP Mmj6V2FPT0WU
NCI-H2126 Mo\GS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUjJR|UxRTN3OD61NlEh|ryP M2D1T3NCVkeHUh?=
DMS-53 NIfLSIVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV;JR|UxRTN3OD63NVEh|ryP NUm0eXhSW0GQR1XS
NCI-H1838 MmXxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWXJR|UxRTN3OT65PFch|ryP NY\nXm5QW0GQR1XS
SJSA-1 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWLJR|UxRTN4MD65N|kh|ryP MlrqV2FPT0WU
ChaGo-K-1 NFnUO2hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mlz6TWM2OD1|NkOuPVI1KM7:TR?= NGrMUnhUSU6JRWK=
IST-SL2 M{jkVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEHMSlhKSzVyPUO3Nk46OjZizszN NWLMcol2W0GQR1XS
U-266 NGDi[2NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml:wTWM2OD1|N{OuNVMzKM7:TR?= Mkm1V2FPT0WU
P31-FUJ NYD1PGhIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHXaVYhKSzVyPUO3Ok46PjRizszN MYXTRW5ITVJ?
HCC2157 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlL1TWM2OD1|N{iuNVYyKM7:TR?= M{HMV3NCVkeHUh?=
COR-L88 NH3MelJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4jpN2lEPTB;M{izMlY4PyEQvF2= MUHTRW5ITVJ?
NCI-H596 M3S0VGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoXlTWM2OD1|OEWuN|Qh|ryP NETod3RUSU6JRWK=
GOTO MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mo\JTWM2OD1|OUCuOlQyKM7:TR?= MoDOV2FPT0WU
NCI-H719 NE\aSHJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlraTWM2OD1|OUGuPFk{KM7:TR?= MknZV2FPT0WU
MN-60 MmDXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUn2XmVSUUN3ME2zPVMvQTR6IN88US=> M2\ScHNCVkeHUh?=
IST-SL1 M{fHSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1LTV2lEPTB;M{m0MlQxPiEQvF2= MXPTRW5ITVJ?
TE-6 NVXrZmVuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYjjV3N[UUN3ME2zPVcvOjV2IN88US=> NYr0c4NbW0GQR1XS
SW962 NHPvdlhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEjOXlRKSzVyPUO5PE43QSEQvF2= M1vjOHNCVkeHUh?=
FADU Mle4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUPJR|UxRTRyMz6wPFMh|ryP MkXCV2FPT0WU
ECC4 M3;wUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXfJR|UxRTRyOD60OVgh|ryP MnX2V2FPT0WU
MES-SA M1HTN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXfJR|UxRTRyOT65PEDPxE1? NVLoNXY{W0GQR1XS
NCI-H716 M1zsPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHf1cnBKSzVyPUSxNE4xPzVizszN NIrKbolUSU6JRWK=
GR-ST NUHJNY1qT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEDjclVKSzVyPUSxNE41QTdizszN M3\PUnNCVkeHUh?=
KY821 MlryS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4[2VmlEPTB;NEG0MlA5PiEQvF2= NYrae|U5W0GQR1XS
NCI-H2141 NVvsZ5h4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mm\qTWM2OD12MkSuN|g5KM7:TR?= Mn:0V2FPT0WU
MDA-MB-134-VI MnLqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4DvWWlEPTB;NEOxMlA{OyEQvF2= NInUR|JUSU6JRWK=
RH-1 NGrLUHhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoH6TWM2OD12M{WuNFkh|ryP M{KxcXNCVkeHUh?=
AM-38 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M37CemlEPTB;NEO4MlU1KM7:TR?= MoGyV2FPT0WU
BL-70 M3;jdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MofvTWM2OD12M{muOFI6KM7:TR?= MU\TRW5ITVJ?
COLO-684 M2rpOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoHHTWM2OD12NEiuPFg5KM7:TR?= NYHwVJdWW0GQR1XS
DMS-79 Mme0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmfITWM2OD12NEmuOlgh|ryP MYXTRW5ITVJ?
EW-1 M3TENmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXu3cGhlUUN3ME20OVIvQDh6IN88US=> Ml;5V2FPT0WU
MS-1 NXjTNZZ1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWTJR|UxRTR3ND6zPFUh|ryP MVLTRW5ITVJ?
A4-Fuk M2e5dGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnPaTWM2OD12NUSuOVA4KM7:TR?= MU\TRW5ITVJ?
TALL-1 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlTWTWM2OD12NUWuNlk4KM7:TR?= NF60WldUSU6JRWK=
SCLC-21H NIrjS3pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVvwcndCUUN3ME20OVUvPjl7IN88US=> NIPsW5ZUSU6JRWK=
KMS-12-PE MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVy4bWZkUUN3ME20OVYvQDJ{IN88US=> NXTaSGpQW0GQR1XS
TE-441-T M1T0U2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NILQdZRKSzVyPUS1O{4yQDZizszN MXTTRW5ITVJ?
A3-KAW NH3lb41Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1P3[GlEPTB;NE[wMlg1OSEQvF2= M3TTXXNCVkeHUh?=
NCI-H1092 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXzJR|UxRTR4Mz65OFMh|ryP NV;JWI13W0GQR1XS
COR-L279 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXHJR|UxRTR4ND64OFch|ryP MWfTRW5ITVJ?
NCI-SNU-5 MnGxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXLHeWR{UUN3ME20O|IvOjhizszN NU\McZoyW0GQR1XS
JAR MmDlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXnJR|UxRTR5ND63PFgh|ryP MV;TRW5ITVJ?
NCI-H446 Mkj6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVXvT2t5UUN3ME20O|cvODhizszN NWn0WoxyW0GQR1XS
HDLM-2 NVqyd5VXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF61PWdKSzVyPUS4NE45PjlizszN MXXTRW5ITVJ?
D-283MED MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NW[4PJp{UUN3ME20PFgvQTJ2IN88US=> M4HWSHNCVkeHUh?=
NCI-H1395 M{Dtc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2O4OmlEPTB;NEi5MlAxPCEQvF2= M4G3[nNCVkeHUh?=
RPMI-6666 NEHsZ4NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX7pXVVqUUN3ME20PVEvOzl6IN88US=> NFeyTlRUSU6JRWK=
CPC-N NVLxU5NVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXqz[|BQUUN3ME21NFIvODZ|IN88US=> MoGxV2FPT0WU
EC-GI-10 NEHYR5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2H5VmlEPTB;NUCyMlE3QSEQvF2= M3j4SHNCVkeHUh?=
CA46 NInkSmZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYLJR|UxRTVyND60NFch|ryP MmXZV2FPT0WU
EHEB MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYrJR|UxRTVyNz6yNFgh|ryP MmjxV2FPT0WU
RPMI-8402 NHfhdIlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEjUTm5KSzVyPUWxNE4xOjFizszN MkniV2FPT0WU
DU-4475 M1PKdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV7GZ5U{UUN3ME21NVEvOTBzIN88US=> M1nPdHNCVkeHUh?=
P12-ICHIKAWA M2SzRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYfJR|UxRTVzMT60NVYh|ryP NWTNdmpTW0GQR1XS
NCI-H1694 NHvafVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXPye5ZEUUN3ME21NVIvPDN7IN88US=> NUjzfJJTW0GQR1XS
NCI-H2171 NFfocIVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkfsTWM2OD13MUKuO|A2KM7:TR?= MmPBV2FPT0WU
Mo-T NH3zeHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUTl[mxbUUN3ME21NVUvOTF4IN88US=> M1y2[HNCVkeHUh?=
HL-60 M2PDeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4rSOGlEPTB;NUKyMlkzPCEQvF2= MlK3V2FPT0WU
NCI-H1436 NGHnTVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEnP[nJKSzVyPUWyN{4xPjRizszN M1XzV3NCVkeHUh?=
NCI-H510A MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MknsTWM2OD13MkSuOlk3KM7:TR?= NWX4TGpVW0GQR1XS
NCI-H2081 MoKzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV;JR|UxRTV{NT61NVMh|ryP NUflZpVKW0GQR1XS
NB5 NGe3dWdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUfpZXVGUUN3ME21NlYvPDNizszN MYDTRW5ITVJ?
NCI-H2227 NHHZNIJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn\jTWM2OD13M{CuNlU1KM7:TR?= MX;TRW5ITVJ?
HSC-4 Mkj5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1rx[WlEPTB;NUO5MlI6OyEQvF2= MXnTRW5ITVJ?
RL MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4e0TmlEPTB;NUSyMlQ1OiEQvF2= M1GwbHNCVkeHUh?=
EVSA-T MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFHOdHhKSzVyPUW0N{43QDRizszN MkPEV2FPT0WU
NCI-H889 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUPTV5M1UUN3ME21OFcvOjNizszN MYTTRW5ITVJ?
COLO-320-HSR Mlq3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGXjRppKSzVyPUW1NU4{PSEQvF2= MWrTRW5ITVJ?
EW-11 MnfOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXfJR|UxRTV3Mj6zPVkh|ryP NIXsVIlUSU6JRWK=
UACC-812 NUXXTXlxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4HlfWlEPTB;NUW5MlM6OSEQvF2= MVPTRW5ITVJ?
CAL-148 NIfyZo5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX\JR|UxRTV4NT6wPFkh|ryP NV\sRo12W0GQR1XS
K052 NUTuO|FGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYnRXo9UUUN3ME21O|kvOjF|IN88US=> M33JeXNCVkeHUh?=
L-428 M1zaOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXTJR|UxRTZyMD62O|Eh|ryP MXHTRW5ITVJ?
LP-1 MnrhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M13MVWlEPTB;NkCzMlU5PyEQvF2= MnriV2FPT0WU
ML-2 NVzUNXJGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlSzTWM2OD14ME[uOVc6KM7:TR?= Mn3vV2FPT0WU
KARPAS-45 NVK2O45IT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX;JR|UxRTZzOD6yNlEh|ryP MmXJV2FPT0WU
HT NFy4R2RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3HEdGlEPTB;NkKyMlc5PSEQvF2= NWTxbGdZW0GQR1XS
NCI-H226 M2jxcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmnPTWM2OD14MkiuN|U{KM7:TR?= MkXFV2FPT0WU
L-363 NE\XfZBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXzYR3BjUUN3ME22OFgvODZ2IN88US=> M1O0eXNCVkeHUh?=
IM-9 NGe4fFFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXTMemtbUUN3ME22OVEvPTRzIN88US=> NU[0ZoV7W0GQR1XS
TUR Ml;FS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnXaTWM2OD14N{GuN|IzKM7:TR?= MkTXV2FPT0WU
NCI-SNU-16 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MULJR|UxRTZ6MD60OlIh|ryP NWrZbHBwW0GQR1XS
NCI-SNU-1 NV35VFYxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXnMZXFGUUN3ME22PVAvOTd{IN88US=> NXvpXGpwW0GQR1XS
U-87-MG NVuyNYF7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlHRTWM2OD14OUSuOVg2KM7:TR?= MUfTRW5ITVJ?
KM-H2 NWTHTI5vT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{Hm[WlEPTB;Nkm5MlA2QSEQvF2= MV\TRW5ITVJ?
DEL MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWPJR|UxRTdyMD64N|gh|ryP NEn5epBUSU6JRWK=
KARPAS-422 NI\NNIFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGXEPWlKSzVyPUe4PU4{OTVizszN MYrTRW5ITVJ?
BC-3 Mnz3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVrSfoY6UUN3ME24NFUvOjB|IN88US=> M4O2UXNCVkeHUh?=
NCI-H1155 M2i2emdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3PnWWlEPTB;OEG4MlA2QSEQvF2= Mkj1V2FPT0WU
NCI-H1581 MnjpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkG5TWM2OD17MECuNlMyKM7:TR?= NIDSZZdUSU6JRWK=

... Click to View More Cell Line Experimental Data

In vivo Oral administration of CHIR-99021 at 30 mg/kg enhances glucose metabolism in a rodent model of type 2 diabetes, with a maximal plasma glucose reduction of nearly 150 mg/dl 3-4 hours after administration, while plasma insulin remains at or below control levels. Oral administration of CHIR-99021 at 16 or 48 mg/kg 1 hour before oral glucose challenges in ZDF rats significantly improves glucose tolerance with 14% and 33% reduction in plasma glucose at 16 mg/kg and 48 mg/kg, respectively, and the higher dose of CHIR-99021 also reduces hyperglycemia before the oral glucose challenge. [1] Administration of CHIR-99021 significantly augments hematopoietic repopulation in recipient mice transplanted with mouse or human hematopoietic stem cells (HSCs), suggesting that GSK-3 is a specific modulator of HSC activity. [4]

Protocol

Kinase Assay:

[1]

+ Expand

Cell-free kinase assays:

Polypropylene 96-well plates are filled with 300 μL/well buffer (50 mM tris HCl, 10 mM MgCl2, 1 mM EGTA, 1 mM dithiothreitol, 25 mM β-glycerophosphate, 1 mM NaF, 0.01% BSA, pH 7.5) containing 27 nM GSK-3α or 29 nM GSK-3β, and 0.5 μM biotin-CREB peptide substrate. Various concentrations of CHIR-99021 are added in 3.5 μL of DMSO, followed by 50 μL of ATP stock to yield a final concentration of 1 μM ATP in all cell-free assays. After incubation, triplicate 100-μL aliquots are transferred to Combiplate eight plates containing 100 μL/well 50 μM ATP and 20 mM EDTA. After 1 hour, the wells are rinsed five times with PBS, filled with 200 μL of scintillation fluid, sealed, left 30 minutes, and counted in a scintillation counter. All steps are performed at room temperature.
Cell Research:

[3]

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  • Cell lines: INS-1E
  • Concentrations: Dissolved in DMSO, final concentrations ~ 20 μM
  • Incubation Time: 1, or 4 days
  • Method:

    Cells are maintained for 24 hours in starvation medium (culture medium with only 5 mM glucose, 1% fetal calf serum). Then cells are exposed to various concentrations of CHIR-99021 for 1, or 4 days. Cell number is measured by staining of cellular DNA with CyQuant dye, which becomes fluorescent when bound to DNA. Fluorescence is measured after 30 minutes of incubation using the FLUOstar Optima reader. Cell replication is determined by BrdUrd incorporation. BrdUrd labeling solution is added to the medium for the last 4 hours before the cells are fixed using FixDenat solution and incubated with monoclonal anti-BrdUrd-POD antibodies. After substrate solution is added to each well, the light emission is measured in a microplate luminometer using the Analyst HT detection system.


    (Only for Reference)
Animal Research:

[1]

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  • Animal Models: Female db/db mice or male ZDF rats with type 2 diabetes
  • Formulation: Formulated as solutions in 20 mM citrate-buffered 15% Captisol or as fine suspensions in 0.5% carboxymethylcellulose.
  • Dosages: ~48 mg/kg
  • Administration: Orally
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 93 mg/mL (185.33 mM)
Ethanol 2 mg/mL (3.98 mM)
Water <1 mg/mL
In vivo 5% DMSO+30% PEG 300+ddH2O 24mg/mL

* 1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 501.8
Formula

C22H18Cl2N8.HCl

CAS No. 252917-06-9 (free base)
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID