Catalog No.S2386 Synonyms: NSC 105327

Indirubin Chemical Structure

Molecular Weight(MW): 262.26

Indirubin is a potent cyclin-dependent kinases and GSK-3β inhibitor with IC50 of about 5 μM and 0.6 μM.

Size Price Stock Quantity  
In DMSO USD 70 In stock
USD 70 In stock
USD 270 In stock
USD 670 In stock
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1 Customer Review

  • Migration was performed in a Boyden chamber and chemotaxis was quantified by counting the migrated cells. Photo images of migration of RA FLS treated with indirubin.

    Inflamm Res, 2017, 66(5):433-440. . Indirubin purchased from Selleck.

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Biological Activity

Description Indirubin is a potent cyclin-dependent kinases and GSK-3β inhibitor with IC50 of about 5 μM and 0.6 μM.
GSK-3β [2]
CDK2/CyclinA [1] CDK5/p35 [1] CDK1/CyclinB [1] CDK4/CyclinD1 [1]
0.6 μM 2.2 μM 5.5 μM 10 μM 12 μM
In vitro

Indirubin is the active ingredient of Danggui Longhui Wan, a mixture of plants that is used in traditional Chinese medicine to treat chronic diseases. Indirubin inhibits CDKs activity with IC50 of 2.2 - 10 μM resulting in cell cycle arrest in the G2/M phase. [1] Indirubin also inhibits GSK-3β with an IC50 of 0.6 μM, attenuating CDK5- and GSK-3β-mediated tau phosphorylation, a process over-active in Alzheimer disease states. [2] It also suppresses tumor necrosis factor (TNF)-induced NF-κB activation in a dose- and time-dependent manner. Indirubin also suppresses the NF-κB activation induced by various inflammatory agents and carcinogens. Indirubin blocks the phosphorylation and degradation of IκBα through the inhibition of activation of IκBα kinase and phosphorylation and nuclear translocation of p65. [3]


Solubility (25°C)

In vitro DMSO 53 mg/mL (202.08 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 262.26


CAS No. 479-41-4
Storage powder
Synonyms NSC 105327

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01735864 Unknown status Psoriasis Vulgaris Chang Gung Memorial Hospital|National Science Council, Taiwan November 2012 --

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID