XAV-939

Catalog No.S1180 Synonyms: NVP-XAV939

XAV-939 Chemical Structure

Molecular Weight(MW): 312.31

XAV-939 selectively inhibits Wnt/β-catenin-mediated transcription through tankyrase1/2 inhibition with IC50 of 11 nM/4 nM in cell-free assays, regulates axin levels and does not affect CRE, NF-κB or TGF-β.

Size Price Stock Quantity  
In DMSO USD 91 In stock
USD 70 In stock
USD 170 In stock
USD 270 In stock
USD 770 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

7 Customer Reviews

  • Fluorescence microscopy of pSuper or Cdo shRNA expressing P19 cells at ITS1 immunostained withβ-tubulin III antibodies. Size bar=100 um. P19/control or P19/Cdo shRNA cells were treated with DMSO or XAV939 in the differentiation medium for 72 h followed by immunostaining.

    Nat Commun 2014 5, 5455. XAV-939 purchased from Selleck.

    (I) Effect of XAV-939 on gefitinib efficacy in indicated NSCLC cells was detected by MTT assay (J) The indicated NSCLC cells were treated with gefitinib in the presence or absence of XAV-939, and then subjected to immunoblot analysis using the indicated antibodies. Data represent the mean ± SD of three independent experiments. *P < 0.05.

    cancer lett, 2016.. XAV-939 purchased from Selleck.

  • Images of the hfVM experiment described in C, comparing the effects of LIF-nano versus empty-nano at equivalent dilution (left panel) and XAV-nano versus empty-nano at equivalent dilution (right panel). Scale bars: 200 μm.

    Dis Model Mech 2014 7(10), 1193-203. XAV-939 purchased from Selleck.

    Pathology observation of mice liver sections stained with hematoxylin and eosin (H&E) and Masson (×200), and the levels of -SMA and Tmem88 were analyzed by immunohistochemistry (×200).

    Mol Immunol, 2016, 80:58-67. XAV-939 purchased from Selleck.

  • Cells plated at 2 x 104 in 24 multiwell with DMEM 10% FBS preconditioning 24 hrs (pretreatment without wnt) switch medium from 2% HS + WNT 100 ng/ml

    Dr. Marco Quarta of Stanford University. XAV-939 purchased from Selleck.

     

    Fig. 1.  Canonical Wnt Signaling Inhibits Prostatic Bud Number Similar to TCDD. E14.5 male UGSs were cultured for 4 days in media containing 10 nM DHT with either vehicle 1 nM TCDD; recombinant DKK1 + DKK2 (500 ng/ml each); or 10 μM XAV-939 to inhibit canonical Wnt signaling. Buds were visualized by performing IHC specific for ecadherin, an epithelium marker (green).  The number of prostatic buds was determined by confocal microscopy. Yellow arrowheads indicate areas where buds are present. U indicates urethra.  Results are mean ±SE for at least four litter-independent samples per treatment. Asterisk indicates a significant decrease compared to control p < 0.05.

    XAV-939 purchased from Selleck.

  • XAV-939 purchased from Selleck.

Purity & Quality Control

Choose Selective Wnt/beta-catenin Inhibitors

Biological Activity

Description XAV-939 selectively inhibits Wnt/β-catenin-mediated transcription through tankyrase1/2 inhibition with IC50 of 11 nM/4 nM in cell-free assays, regulates axin levels and does not affect CRE, NF-κB or TGF-β.
Targets
TNKS2 [1]
(Cell-free assay)
TNKS1 [1]
(Cell-free assay)
4 nM 11 nM
In vitro

XAV-939 specifically inhibits tankyrase PARP activity. XAV-939 dramatically decreases DNA-PKcs protein levels, confirming the critical role of tankyrase poly-ADP-ribosylation activity in maintaining stability of the DNA-PKcs protein. The greatest reduction of DNA-PKcs protein levels (< 25% relative expression compared to DMSO treated controls) occurs at 12 hours with 1.0 μM XAV-939 exposure. Treatment of human lymphoblasts with 1.0 μM XAV-939 results in marked elevation of tankyrase 1 levels. [1] XAV-939 is axin stabilizing agent. XAV-939 stimulates beta-catenin degradation by stabilizing axin, the concentration-limiting component of the destruction complex. XAV-939 stabilizes axin by blocking the poly-ADP-ribosylating enzymes tankyrase 1 and tankyrase 2. Both tankyrase isoforms interact with a highly conserved domain of axin and stimulate its degradation through the ubiquitin-proteasome pathway. XAV-939 deregulates the Wnt/b-catenin pathway which has been implicated in many cancers. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Sf-21 M3\iUmtqdmG|ZTDBd5NigQ>? MmjxNVgvPzVizszN MmLpOlAhdWmw NWLPdVhWTE2VTx?= MnG5TY5pcWKrdHnvckBw\iCQLYTldo1qdmGuIFfTWE11[WepZXSgWG5MWzJiZYjwdoV{e2WmIIfpeIghUUN3MDDv[kAxNjByNUOg{txO M{\EZlI{QDd7NEOx
HEK293T NFK0bVFHfW6ldHnvckBCe3OjeR?= M{D2TmROW09? M33BNGlvcGmkaYTpc44hd2ZiV370JJNq\26jbHnu[{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJIZwenOtb3zpck1qdmS3Y3XkJINCVVBicnXzdI9ve2ViZXzlcYVvfCCjY4TpeoF1cW:wIIfpeIghUUN3MDDv[kAxNjB5ODFOwG0> MVqyN|g4QTR|MR?=
HEK293T MV3GeY5kfGmxbjDBd5NigQ>? MlfxNVAh|ryP NVX1SFNJTE2VTx?= MX;Jcohq[mm2aX;uJI9nKGKndHGtZ4F{\WmwLXTldIVv\GWwdDDjZY5wdmmlYXygW451OyCyYYToe4F6KHerdHigTWM2OCCxZjCwMlA2OSEQvF2= NH;zWJAzOjF7MUW1Oy=>
HEK293T MnHFSpVv[3Srb36gRZN{[Xl? M2[yfFI1KGh? NITxO3ZFVVOR MW\Jcohq[mm2aX;uJI9nKG2xdYPlJHdvfDODIIPp[45idGmwZzD3bZRpKEmFNUCgc4YhOC5yN{ig{txO MnPBNlIzPjB{MEO=
SW480 M{T2dWZ2dmO2aX;uJGF{e2G7 NGXLXYEyOCEQvF2= MUCyOEBp MXHEUXNQ MmruV5Ri[mmuaYrheIlwdiCxZjDBfIlvOiC5aYToJGVEPTBib3[gNE4{PzFizszN M2\lfVIzOjZyMkCz
HEK293T MVTGeY5kfGmxbjDBd5NigQ>? MXq1NEDPxE1? NEXJZW5FVVOR NVG0OYpYUGG|IH7vJGVn\mWldDDvckBnd3K|a3;sbY4ucW6mdXPl[EBkSU2SIIPp[45idGmwZzDpckBpfW2jbjDISWszQTOWIHPlcIx{KGOxZYjwdoV{e2mwZzDDVmU> NH3GWY8zOjJ4MEKwNy=>
IEC-6 MXLGeY5kfGmxbjDBd5NigQ>? M2LIOlYhcA>? M1PTeGFvfGGpb37pd5Qh[WO2aY\peJkh[XRiQnX0ZU1k[XSnbnnuM3RETiCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKFewdD2zZU1qdmS3Y3XkJIF5cW5{IHX4dJJme3Orb36ge4l1cCCLQ{WwJI9nKDBwNkSg{txO Mn;lNlQxPjB2OEm=
IEC-6 MkXQSpVv[3Srb36gRZN{[Xl? MUG2JIg> NGjkS|BCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IFLleIEu[2G2ZX7pck9VS0ZiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCZboStN4EucW6mdXPl[EBt\3J3IHX4dJJme3Orb36ge4l1cCCLQ{WwJI9nKDJwOTFOwG0> MlvGNlQxPjB2OEm=
DLD1 MVrGeY5kfGmxbjDBd5NigQ>? NXW4T5E3OjBizszN NULVXYVjOjRiaB?= NXvZcI5{TE2VTx?= M13OTmlvcGmkaYTpc44hd2ZidHHub5lz[XOnIHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiVFPGMYRmeGWwZHXueEB1emGwc3PybZB1cW:wYXygZYN1cX[rdIm= MUiyOFUzPzd7Mh?=
DLD1 M4TNb2N6fG:2b4jpZ{BCe3OjeR?= M4HiZVIxKM7:TR?= NUDGRndXOTBiZB?= MkSySG1UVw>? MWfDfZRwfG:6aXPpeJkh[XO|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25? NHHOeHAzPDV{N{e5Ni=>
VERO NI[4NHNHfW6ldHnvckBCe3OjeR?= MlH4NlUh|ryP MX\EUXNQ MWXEbZN1fXKkZYOgVGFTKGKnbISgd5lvfGinc3nzMEBi\m[nY4TpcochfGinIHHjeIlvKGO7dH;zb4Vt\XSxbjygZ4VtdCC|aHHw[UBidmRiY3XscEBi\Ginc3nvci=> NXfsVopjOjV|M{K4OFU>
HeLa MXHGeY5kfGmxbjDBd5NigQ>? NEDPflQyOCEQvF2= NHfvO3A1QCCq NH:2O5dT\WS3Y4Tpc44hd2ZiY4n0c5Bt[XOvaXOg[Il{fHKrYoX0bY9vKGGwZDDueYNt\WG{IITyZY5{dG:lYYTpc44hd2ZizsKtZ4F1\W6rbh?= MmHvNlUxPjF2OUm=
SiHa MnrjSpVv[3Srb36gRZN{[Xl? NUnsUpViOTBizszN Mo\DOFghcA>? MXzS[YR2[3Srb36gc4Yh[3m2b4DsZZNucWNiZHnzeJJq[nW2aX;uJIFv\CCwdXPs[YFzKHS{YX7zcI9k[XSrb36gc4Yh|rJvY3H0[Y5qdg>? MonGNlUxPjF2OUm=

... Click to View More Cell Line Experimental Data

Protocol

Cell Research:[1]
+ Expand
  • Cell lines: WTK1 lymphoblasts
  • Concentrations: 1.0 μM
  • Incubation Time: 8 hours
  • Method: XAV-939 is solubilized in DMSO at 55 °C to make a 10 mM stock solution which may be diluted later to a working concentration of 100 μM. WTK1 lymphoblasts treated with either DMSO or 1.0 μM XAV-939 for 8 hours are loaded into independent wells of a 4-20% gradient SDS-PAGE every 2 hours over the course of 6 hours. At each time point, DMSO and XAV-939 samples are loaded into wells immediately adjacent to the prior time point. The corresponding load times at 0, 2 and 4 hours results in total run times of 2, 4 and 6 hours respectively. The gel is analyzed via western blot for DNA-PKcs following completion of the final run time and is quantified after normalization to actin loading controls.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 12 mg/mL (38.42 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents individually and in order:
4% DMSO+corn oil
1mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 312.31
Formula

C14H11F3N2OS

CAS No. 284028-89-3
Storage powder
Synonyms NVP-XAV939

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    I want to inject XAV 939 (Cat # S1180) into mice through I.P. and just wonder what kind of solvent/solution I can use for this.

  • Answer:

    S1180 XAV-939 can be dissolved in 4% DMSO+corn oil at 1 mg/ml as a clear solution. When preparing the solution, please dissolve the compound in DMSO clearly first. You can sonicate and warm it in water bath at about 45 degree to help dissolving. Then dilute with corn oil.

Wnt/beta-catenin Signaling Pathway Map

Related Wnt/beta-catenin Products

Tags: buy XAV-939 | XAV-939 supplier | purchase XAV-939 | XAV-939 cost | XAV-939 manufacturer | order XAV-939 | XAV-939 distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID