PJ34 HCl

Catalog No.S7300

PJ34 HCl  Chemical Structure

Molecular Weight(MW): 331.8

PJ34 HCl is the hydrochloride salt of PJ34, which is a PARP inhibitor with EC50 of 20 nM and is equally potent to PARP1/2.

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USD 470 In stock
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2 Customer Reviews

  • Effect of PJ34 treatment on expression of IL-1ß, IL-6 and TNF-α at 24h after SAH.(A) Representative Western blots showing levels of IL-1ß, IL-6 and TNF-α. (B)-(D) The relative band densities of IL-1ß,IL-6 and TNF-α. The densities of the protein bands were analyzed and normalized to ß-actin. The bars represent the mean±SD. n=6. *p<0.05 vs sham, #p<0.05 vs SAH+Vehicle.

    Brain Res, 2016, 1644:32-8. PJ34 HCl purchased from Selleck.

    Effect of PJ34 treatment on expression of MMP-9, occludin and claudin-5 at 24 h after SAH. (A) Representative Western blots showing levels of MMP-9, occludin and claudin-5. (B)-(D) The relative band densities of MMP-9, occludin and claudin-5. The densities of the protein bands were analyzed and normalized to ß-actin. The bars represent the mean±SD. n=6. *p<0.05 vs sham, #p<0.05 vs SAH+Vehicle.

    Brain Res, 2016, 1644:32-8.. PJ34 HCl purchased from Selleck.

Purity & Quality Control

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Biological Activity

Description PJ34 HCl is the hydrochloride salt of PJ34, which is a PARP inhibitor with EC50 of 20 nM and is equally potent to PARP1/2.
Features Water-soluble PARP1/2 inhibitor with >10,000-fold potentcy vs. 3-aminobenzamide (prototypical PARP inhibitor). Potential uses in cardiovascular diseases (stroke, cerebral ischemia, & myocardial ischemia).
Targets
PARP [1]
(Cell-free assay)
20 nM(EC50)
In vitro

PJ34 is a potent, phenanthridinone PARS inhibitor, which is approximately 10,000 times more potent than the prototypical PARS inhibitor 3-aminobenzamide. PJ34 inhibited peroxynitrite-induced cell necrosis with EC50 of 20 nM. PJ34 provides cardioprotection by decreasing myocardial infarct size and enhancing postischemic regional and global functional recovery. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HeLa cells NWfhSnlUTnWwY4Tpc44h[XO|YYm= NEjSUYpKdmirYnn0bY9vKG:oIGDBVnAyKGmwIHj1cYFvKEinTHGgZ4VtdHNiYomg[ox2cWRic3PpcpRqdGyjdHnvckBkd3WwdHnu[{B2e2mwZzDbZYRmdnmuYYTl[E0{OlCfTlHEJIF{KHO3YoP0doF1\SxiSVO1NF0xNjB{IN88US=> MnS0NVg4OTN4NkW=
Escherichia coli Rosetta2 (DE3) cells MUHGeY5kfGmxbjDhd5NigQ>? M4j4PWlvcGmkaYTpc44hd2ZiaIXtZY4hVi22ZYLtbY5idCB4eHjpd{11[WepZXSgRXJVTDZiKEi3N{B1dyBzMU[xLUBmgHC{ZYPz[YQhcW5iRYPjbIVzcWOqaXGgZ49tcSCUb4PleJRiOiBqRFWzLUBk\WyuczD1d4lv\yCQQVSrJIF{KHO3YoP0doF1\SCkeTDmcJVwemW|Y3XuZ4Uh[XO|YYmsJGlEPTB;MD6yNVg4QCEQvF2= MnLBNlQ6ODB5N{C=
Escherichia coli BL21 Star (DE3) cells NHLLWVVHfW6ldHnvckBie3OjeR?= NX;jTldMUW6qaXLpeIlwdiCxZjC2fIhqey22YXfn[YQhSVKWREWgLJVvc26xd36gc5Jq\2mwKTDlfJBz\XO|ZXSgbY4hTXOlaHXybYNpcWFiY3;sbUBDVDJzIGP0ZZIhMESHMzmgZ4VtdHNuIFnDOVA:OC53NzFOwG0> NXLwU5k2OjR7MEC3O|A>
MEF cells MlTrVJJwdGmoZYLheIlwdiCjc4PhfS=> M3;rdVczKGh? MYTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IF3FSkBk\WyuczDoZZJjd3KrbnegRpJk[TFiZHXs[ZRqd25ibYX0ZY51KGG2IHX4c44hOTFiYYPz[ZN{\WRiYYOgZ4VtdCC4aXHibYxqfHliYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0yOy5{IN88US=> MVGyNlM3PTV4Mx?=
MEF cells M4\TNHBzd2yrZnXyZZRqd25iYYPzZZk> MWS3NkBp M{DkZ2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4Sge4lt\CC2eYDlJG1GTiClZXzsd{Bie3Onc4Pl[EBieyClZXzsJJZq[WKrbHn0fUBi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUG5Mlgh|ryP NFi5S2gzOjN4NUW2Ny=>

... Click to View More Cell Line Experimental Data

In vivo PJ34 suppresses the development of clinical signs of EAE in MBP-immunized PLSJL mice. PJ34 exerted therapeutic effects at the onset of EAE that are associated with reduced CNS inflammation and the maintenance of neurovascular integrity. PJ34 partially inhibits the expression of TNF-α and ICAM-1 in the Spinal Cord Tissues of MBP-Immunized Mice.[2] PJ34 provides significant, dose-dependent, anti-inflammatory effects in a variety of local inflammation models. PJ34 dose-dependently suppresses neutrophil infiltration and nitric oxide (but not KC and IL-1β) production in peritonitis. In a model of systemic endotoxemia, PJ34 pretreatment significantly reduces plasma levels of TNF-α, IL-1β and nitrite/nitrate (breakdown products of nitric oxide) production. PJ34 treatment (oral gavage) induces a significant suppression of the inflammatory response in dextran sulfate colitis, multiple low dose streptozotocin diabetes and cyclophosphamide-accelerated autoimmune diabetes in the non-obese diabetic mice, and reduces the degree of mononuclear cell infiltration into the iris in an endotoxin-induced uveitis model. [3]

Protocol

Animal Research:[2]
+ Expand
  • Animal Models: Female PLSJL mice
  • Formulation: Saline
  • Dosages: 10 mg/kg b.wt. twice daily
  • Administration: orally
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 66 mg/mL (198.91 mM)
Water 66 mg/mL (198.91 mM)
Ethanol Insoluble
In vivo Add solvents individually and in order:
30% PEG400+0.5% Tween80+5% propylene glycol
15 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 331.8
Formula

C17H17N3O2.HCl

CAS No. 344458-15-7
Storage powder
Synonyms N/A

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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PARP Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID