Thiazovivin

Catalog No.S1459

Thiazovivin Chemical Structure

Molecular Weight(MW): 311.36

Thiazovivin is a novel ROCK inhibitor with IC50 of 0.5 μM in a cell-free assay, promotes hESC survival after single-cell dissociation.

Size Price Stock Quantity  
In DMSO USD 150 In stock
USD 120 In stock
USD 210 In stock
USD 670 In stock

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Notes:

2. For more details, such as half maximal inhibitory concentrations (IC50s) and working concentrations of each inhibitor, please click on the link of the inhibitor of interest.
3. "+" indicates inhibitory effect. Increased inhibition is marked by a higher "+" designation.
4. Orange "√" refers to compounds which do inhibitory effects on the related isoform, but without specific value.

Biological Activity

Description Thiazovivin is a novel ROCK inhibitor with IC50 of 0.5 μM in a cell-free assay, promotes hESC survival after single-cell dissociation.
Targets
ROCK [1]
(Cell-free assay)
~0.5 μM
In vitro

Although displaying little impact on cell proliferation, Thiazovivin treatment significantly enhances the survival of human embryonic stem cells (hESCs) after enzymatic dissociation more than 30-fold, while homogenously maintaining pluripotency with the characteristic colony morphology, expression of typical pluripotency markers such as alkaline phosphatase (ALP), and normal karyotype. Dissociated hESCs treated with Thiazovivin display dramatically increased adhesion to matrigel- or laminin-coated plates but not to gelatin-coated plates within a few hours. Thiazovivin treatment increases cell-ECM adhesion-mediated β1 integrin activity, which synergizes with growth factors to promote cell survival. In addition to activating integrin, Thiazovivin but not Tyrintegin (Ptn) protects hESCs from death in the absence of ECM in suspension through E-cadherin-mediated cell-cell interaction. Thiazovivin treatment potently inhibits endocytosis of E-cadherin, consequently stabilizing E-cadherin on the cell surface and leading to reestablishment of cell-cell interaction, which is essential for hESC survival in ECM-free conditions. Thiazovivin but not Tyrintegin (Ptn) at 2 μM inhibits Rho-associated kinase (ROCK) activity and protects hESCs at a similar level as the widely used selective ROCK inhibitor Y-27632 at 10 μM, suggesting that Rho-ROCK signaling regulates cell-ECM and cell-cell adhesion. [1] Thiazovivin at 1 μM increases the reprogramming efficiency of CB mononuclear cells to induced pluripotent stem cells (iPSCs) by more than 10 times. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
SCU-i10 hiPSCs NH3TcHpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGTPTYwxNTJyIN88US=> NW\aVHRtOjRiaB?= MoLmd4lodmmoaXPhcpRtgSCvb3T1cIF1\XNic3nu[4xmNWOnbHygdIxifGmwZzDl[oZq[2mnbnP5JIFv\CCycn;tc5RmKE6FTTDndo94fGh? MVqyOVA4Pzl|Mh?=
HSMCs NFPGbFdHfW6ldHnvckBCe3OjeR?= MYCx5qCK|ryP NITRXpQzKGh? MWHy[ZZmenOnczDBcodKUS2rbnT1Z4VlKE2\UGSxJJBpd3OyaH;yfYxifGmxbh?= NEjIcnQzPDl4NUG3NC=>

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Protocol

Solubility (25°C)

In vitro DMSO 15 mg/mL (48.17 mM)
Water <1 mg/mL
Ethanol <1 mg/mL
In vivo 30% PEG400+0.5% Tween80+5% propylene glycol 30 mg/mL

* 1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 311.36
Formula

C15H13N5OS

CAS No. 1226056-71-8
Storage powder
in solvent
Synonyms N/A

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID