Pazopanib HCl (GW786034 HCl)

Catalog No.S1035

Pazopanib HCl (GW786034 HCl) Chemical Structure

Molecular Weight(MW): 473.98

Pazopanib HCl (GW786034 HCl) is a novel multi-target inhibitor of VEGFR1, VEGFR2, VEGFR3, PDGFR, FGFR, c-Kit and c-Fms with IC50 of 10 nM, 30 nM, 47 nM, 84 nM, 74 nM, 140 nM and 146 nM in cell-free assays, respectively.

Size Price Stock Quantity  
In DMSO USD 191 In stock
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USD 270 In stock
USD 370 In stock
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3 Customer Reviews

  • IC50 of Pazopanib that block ANDV-induced EC permeability. Endothelial cells were ANDV infected, and 3 days postinfection the permeability of cells in response to VEGF addition was determined in the presence or absence of increasing amounts of kinase inhibitor. (B): VEGFR2-Src inhibitors block ANDV-induced permeability. Endothelial cells were plated on vitronectin-coated Transwell inserts and infected at an MOI of 0.5 in triplicate with ANDV. Three days postinfection, the permeability of ANDV- and mock-infected endothelial cell monolayers was determined at indicated times in the presence or absence of Pazopanib.

    J Virol, 2011, 85(5): 2296-303. Pazopanib HCl (GW786034 HCl) purchased from Selleck.

    MRC5 non-transformed human lung fibroblasts were infected with influenza viruses (100 multiplicity of infection). Two hours after infection the cells were treated with vehicle control; sorafenib tosylate (2mM); pazopanib (2mM); OSU-03012 (2mM); and AR-13 (2mM). Twenty-four hours after infection the cells are treated with live/dead agent where green cells are viable and cells staining yellow or red are considered dead. Cells are examined at 10 magnification in a Hermes wide-field microscope (n¼3 SEM) P<0.05 less than vehicle control level of virus-mediated cell killing.

    J Cell Physiol, 2016, 231(10):2286-302.. Pazopanib HCl (GW786034 HCl) purchased from Selleck.

  • Effect of HDIL-2/TKI on apoptosis of RCC cells. Three RCC cell lines treated with different concentrations of Pazopanib and HDIL-2 and incubated for 48 h. Microscopic images show apoptotic materials 48 h following treatment (arrows show the apoptotic materials in the pazopanib-treated cells).

    Expert Opin Pharmacother 2014 15(11), 1489-99. Pazopanib HCl (GW786034 HCl) purchased from Selleck.

Purity & Quality Control

Choose Selective VEGFR Inhibitors

Biological Activity

Description Pazopanib HCl (GW786034 HCl) is a novel multi-target inhibitor of VEGFR1, VEGFR2, VEGFR3, PDGFR, FGFR, c-Kit and c-Fms with IC50 of 10 nM, 30 nM, 47 nM, 84 nM, 74 nM, 140 nM and 146 nM in cell-free assays, respectively.
Features A multi-kinase inhibitor.
Targets
VEGFR1 [1]
(Cell-free assay)
VEGFR2 [1]
(Cell-free assay)
VEGFR3 [1]
(Cell-free assay)
FGFR [1]
(Cell-free assay)
PDGFR [1]
(Cell-free assay)
10 nM 30 nM 47 nM 74 nM 84 nM
In vitro

Pazopanib potently inhibits VEGF-induced phosphorylation of VEGFR2 in HUVEC cells with IC50 of 8 nM. [1] Pazopanib shows dose-dependent growth inhibition in all synovial sarcoma cell lines including SYO-1 and HS-SY-II cells. Proliferation of SYO-1 and HS-SY-II cells is inhibited even at 1 µg/mL of Pazopanib and is completely abolished at 5 µg/mL. Pazopanib induces G1 arrest, and thereby suppresses the growth of synovial sarcoma cells. Phosphorylation of Akts, GSK-3β, JNKs, p70 S6 Kinase, and mTOR is suppressed in Pazopanib-treated SYO-1 cells compared with that in the vehicle-treated cells. [2] Pazopanib between 20 m g/mL and 22.5 m g/mL shows an increasing reduction of RPE cell viability. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HUVEC NELCeWZIem:5dHigbY5pcWKrdH;yfUBie3OjeR?= NEPIT5JqdmirYnn0d{B1cGViVlXHSk1qdmS3Y3XkJJBzd2yrZnXyZZRqd25ib3[gTHVXTUO| NI\wNG4yQDZ{MEO4Ni=>
HUVEC Mo[yT4lv[XOnIHHzd4F6 NETzfJRqdmirYnn0d{BXTUeILXnu[JVk\WRicHjvd5Bpd3K7bHH0bY9vKG:oIG\FS2ZTNTJiaX6gTHVXTUNiY3XscJMhf2m2aDDhckBKSzVyIH;mJQKJxDhibl2= M4D5NFE5PjJyM{iy
MM M3;VPWtqdmG|ZTDhd5NigQ>? M1vLW4lvcGmkaYTzJHZGT0ZvaX7keYNm\CCyaH;zdIhwenmuYYTpc44hd2ZiZnz0NS=> NFPobpcyPzF4NEOzNi=>
MM.1S NX7tZWdQT3Kxd4ToJIlvcGmkaYTvdpkh[XO|YYm= M{HkPFExKM7:Zz;tUC=> Mn7sbY5pcWKrdIOgUW0hS2WubDDHdo94fGh? MUCxO|E3PDN|Mh?=
MM.1R NGnSWIxIem:5dHigbY5pcWKrdH;yfUBie3OjeR?= MUCxNEDPxGdxbVy= M{XtfolvcGmkaYTzJG1OKEOnbHygS5Jwf3Sq MmXXNVcyPjR|M{K=
RPMI MlPYS5Jwf3SqIHnubIljcXSxcomgZZN{[Xl? NW\JS3oxOTBizsznM41N Mn;MbY5pcWKrdIOgUW0hS2WubDDHdo94fGh? M4ftO|E4OTZ2M{Oy
Dox40 Ml[2S5Jwf3SqIHnubIljcXSxcomgZZN{[Xl? M2XtN|ExKM7:Zz;tUC=> Mn75bY5pcWKrdIOgUW0hS2WubDDHdo94fGh? NHjYXGgyPzF4NEOzNi=>
INA-6 MX3Hdo94fGhiaX7obYJqfG:{eTDhd5NigQ>? NXzPUHFJOTBizsznM41N NH7V[VNqdmirYnn0d{BOVSCFZXzsJGdzd3e2aB?= MlrqNVcyPjR|M{K=
OPM2 M3vXSmdzd3e2aDDpcohq[mm2b4L5JIF{e2G7 MYGxNEDPxGdxbVy= NWr5cGZicW6qaXLpeJMhVU1iQ3XscEBIem:5dHi= MofmNVcyPjR|M{K=
U266 M3TLZmdzd3e2aDDpcohq[mm2b4L5JIF{e2G7 MWKxNEDPxGdxbVy= MVXpcohq[mm2czDNUUBE\WyuIFfyc5d1cA>? NGXwdYYyPzF4NEOzNi=>
MM.1S MkLlZ5l1d3SxeHnjbZR6KGG|c3H5 MWqyNEDPxGdxbVy= MlvEbY5pcWKrdIOgUW0hS2WubDDTeZJ3cX[jbB?= M2PueFE4OTZ2M{Oy
MM.1R M3S1bYN6fG:2b4jpZ4l1gSCjc4PhfS=> M17OXlIxKM7:Zz;tUC=> MlKzbY5pcWKrdIOgUW0hS2WubDDTeZJ3cX[jbB?= MVixO|E3PDN|Mh?=
RPMI NUHpSZdL[3m2b4TvfIlkcXS7IHHzd4F6 NV7CRlgzOjBizsznM41N MnK0bY5pcWKrdIOgUW0hS2WubDDTeZJ3cX[jbB?= NVPzbZNvOTdzNkSzN|I>
Dox40 Mn;DZ5l1d3SxeHnjbZR6KGG|c3H5 MYKyNEDPxGdxbVy= MUDpcohq[mm2czDNUUBE\WyuIGP1dpZqfmGu NELVbmQyPzF4NEOzNi=>
INA-6 M3y0PYN6fG:2b4jpZ4l1gSCjc4PhfS=> M3PPXVIxKM7:Zz;tUC=> MVfpcohq[mm2czDNUUBE\WyuIGP1dpZqfmGu MXmxO|E3PDN|Mh?=
OPM2 MoPzZ5l1d3SxeHnjbZR6KGG|c3H5 MlL1NlAh|rypL33M NHf2TZdqdmirYnn0d{BOVSCFZXzsJHN2en[rdnHs NEH4UHAyPzF4NEOzNi=>
U266 NFG4VndkgXSxdH;4bYNqfHliYYPzZZk> M1T2clIxKM7:Zz;tUC=> NH74e3RqdmirYnn0d{BOVSCFZXzsJHN2en[rdnHs NWPhTWNrOTdzNkSzN|I>
MM.1S NX34e4hmTnWwY4Tpc44h[XO|YYm= NE\HSIN{fXCycnXzd4V{KF[HR1[tTY5lfWOnZDDFcoRwfGinbHnhcEBE\WyuIGDyc4xq\mW{YYTpc44h[W6mIF3p[5JifGmxbj6= MXixO|E3PDN|Mh?=
MM.1R M1TGeWZ2dmO2aX;uJIF{e2G7 Mn3Jd5VxeHKnc4Pld{BXTUeILVnu[JVk\WRiRX7kc5Rp\WyrYXygR4VtdCCScn;sbYZmemG2aX;uJIFv\CCPaXfyZZRqd25w NH35U5MyPzF4NEOzNi=>
Dox40 M{n2TGZ2dmO2aX;uJIF{e2G7 MWjzeZBxemW|c3XzJHZGT0ZvSX7keYNm\CCHbnTveIhmdGmjbDDD[YxtKFC{b3zp[oVz[XSrb36gZY5lKE2rZ4LheIlwdi5? Mn3TNVcyPjR|M{K=
OPM2 NVnDWphTTnWwY4Tpc44h[XO|YYm= NGLPO2R{fXCycnXzd4V{KF[HR1[tTY5lfWOnZDDFcoRwfGinbHnhcEBE\WyuIGDyc4xq\mW{YYTpc44h[W6mIF3p[5JifGmxbj6= M1X5TlE4OTZ2M{Oy
HBMEC MWXHdo94fGhiaX7obYJqfG:{eTDhd5NigQ>? MnnnglExKM7:TR?= M3rDNmROW09? M4PtT2lEPTB;MTFOwG0> MYSyNVA5OTZ3Nh?=
HBMEC NX7Efo9RTnWwY4Tpc44h[XO|YYm= MmTRglEh|ryP NFrj[ZhFVVOR MXXhZpJw\2G2ZYOgeIhmKHCqb4PwbI9zgWyjdHnvckBw\iCYRVfGVlIhf2m2aDDkbZNzfXC2aX;uJI9nKGSxd37zeJJm[W1iUFzD{tMy NXvaO41vOjFyOEG2OVY>
HBMEC MnPGSpVv[3Srb36gZZN{[Xl? M1jvVp4yKM7:TR?= MnjuSG1UVw>? NFXQeHNlcXO{dYD0d{B1cGViUnHzMXJi\i2HUlugdIF1cHejeTD0bJJwfWeqIHTlZ5Jm[XOnZDDwbI9{eGixconsZZRm\CCPRVuxM|Ih[W6mIFXST|EwOg>? NUnMN5FOOjFyOEG2OVY>
HBMEC M2LzN2Z2dmO2aX;uJIF{e2G7 NH\vZ|J,OjBizszN MYjEUXNQ Ml;2[Il{enWydIOgOVAmKG:oIIT1ZoUh\m:{bXH0bY9vKGG2IEGg{txO M1\IflIyODhzNkW2
MDA-MB-231 M1Hsbmdzd3e2aDDpcohq[mm2b4L5JIF{e2G7 M3vxdZ4yOCEQvF2= NYfNOllmTE2VTx?= MkXwTWM2OD13IN88US=> MXKyNVA5OTZ3Nh?=
MDA-MB-231 M161TmZ2dmO2aX;uJIF{e2G7 M{fpXlAvPSEQvF2= NYfQWpdLTE2VTx?= MmO5bY5pcWKrdIOgeIhmKEWUS{GvNkB{cWewYXzpcocheGG2aIfhfS=> MU[yNVA5OTZ3Nh?=
MDA-MB-231 MnXESpVv[3Srb36gZZN{[Xl? MlvpOUDPxE1? MlTiSG1UVw>? NUXPVm53cW6mdXPld{BiKGOnbHytZ5lkdGViYYLy[ZN1 MXGyNVA5OTZ3Nh?=
J82 MVvjfZRwfG:6aXPpeJkh[XO|YYm= MUf+NVAh|ryP MoW0SG1UVw>? NUHSblV3UUN3ME2yOE42PyEQvF2= NEXBZ4EzOTV{OUmwNC=>
T24 NGTkU2ZkgXSxdH;4bYNqfHliYYPzZZk> NXTuW4M6hjFyIN88US=> MnzESG1UVw>? NFPNcVhKSzVyPUWyMlQ2KM7:TR?= NWO1RXdTOjF3Mkm5NFA>
HT1376 M{HtNIN6fG:2b4jpZ4l1gSCjc4PhfS=> MWf+NVAh|ryP MXjEUXNQ M2PkWmlEPTB;MkiuNlEh|ryP NVG0fXFSOjF3Mkm5NFA>
RT4 MUDjfZRwfG:6aXPpeJkh[XO|YYm= NFzuO2Z,OTBizszN NEexTldFVVOR NYTjUoVuUUN3ME21MlE1KM7:TR?= MojyNlE2Ojl7MEC=
CRL1749 MlHzZ5l1d3SxeHnjbZR6KGG|c3H5 Mm[0glExKM7:TR?= NYG5SVR3TE2VTx?= MYPJR|UxRTJ{Lk[5JO69VQ>? MW[yNVUzQTlyMB?=
HTB9 NGDFPZdkgXSxdH;4bYNqfHliYYPzZZk> NEf5Tnd,OTBizszN MYnEUXNQ MXTJR|UxRTFzLki0JO69VQ>? MoftNlE2Ojl7MEC=
Sup M33uUIN6fG:2b4jpZ4l1gSCjc4PhfS=> M1m3dZ4yOCEQvF2= NIHRTJdFVVOR NUHoXY9lUUN3ME21N{4{OiEQvF2= MUmyNVUzQTlyMB?=
HTB3 MXjjfZRwfG:6aXPpeJkh[XO|YYm= MmnLglExKM7:TR?= MmK1SG1UVw>? NHXWZ4RKSzVyPUG0MlE3KM7:TR?= M{i5eVIyPTJ7OUCw
CEC NXX3O|YxTnWwY4Tpc44h[XO|YYm= MXH+NVAh|rypL33M M3W5cmROW09? Mojv[I94di2{ZXf1cIF1\XNiVlXHSkBt\X[nbIO= M37pVFIyPjJyOEKy
RPE MlrBSpVv[3Srb36gZZN{[Xl? NFTBPGZ,OTBizsznM41N MoTnSG1UVw>? MYXkc5dvNXKnZ4XsZZRmeyCYRVfGJIxmfmWucx?= M3jVUFIyPjJyOEKy
CEC Mn2wSpVv[3Srb36gZZN{[Xl? NGe2U2p,PSEQvHevcWw> M2HNb2ROW09? NIj6PJZjdG:la4Og[Y5ld3SqZXzpZYwh[2WubDDtbYdz[XSrb36= NF24VVkzOTZ{MEiyNi=>
5637 Mo[zS5Jwf3SqIHnubIljcXSxcomgZZN{[Xl? Ml;BSG1UVw>? M3LmdWlEPTB;MUWuNQKBkc7:TR?= NV;WTZJROjN6OEe2NFU>
J82 NVXWbJY4T3Kxd4ToJIlvcGmkaYTvdpkh[XO|YYm= NIHQd2lFVVOR NEf2RnlKSzVyPUG4MlTjiIoQvF2= MViyN|g5PzZyNR?=
5637 MnPVRZV1d3CqYXf5JIF{e2G7 M1X6elIxKM7:TR?= M2nPb2ROW09? MkTJeJJq\2encoOgeIhmKGG3dH;wbIFocWNicILvZ4V{ew>? MkizNlM5QDd4MEW=
J82 MXzBeZRweGijZ4mgZZN{[Xl? M3PKcFIxKM7:TR?= M3vNbWROW09? MmTieJJq\2encoOgeIhmKGG3dH;wbIFocWNicILvZ4V{ew>? MWqyN|g5PzZyNR?=
5637 NYLWcGJyTnWwY4Tpc44h[XO|YYm= NH3QUlczOCEQvF2= M{XURWROW09? MVTpcoR2[2W|IHz5d49{d22jbD3k[ZBmdmSnboSgcoVkem:|aYO= NU[xTHJvOjN6OEe2NFU>
J82 MnfsSpVv[3Srb36gZZN{[Xl? NXP3[m15OjBizszN Ml3WSG1UVw>? MonDbY5lfWOnczDsfZNwe2:vYXyt[IVx\W6mZX70JI5m[3Kxc3nz M4rTblI{QDh5NkC1
5637 M4DIVmZ2dmO2aX;uJIF{e2G7 M4TBN|IxKM7:TR?= MmPnSG1UVw>? NUHtSohLcW6mdXPld{BtgXOxc3;t[UBidHSncnH0bY9vKGGwZDDpcohq[mm2czDDRkBi[3Srdnn0fS=> MVWyN|g5PzZyNR?=
J82 MUTGeY5kfGmxbjDhd5NigQ>? MlzZNlAh|ryP Mn\6SG1UVw>? NHzZd3RqdmS3Y3XzJIx6e2:|b33lJIFtfGW{YYTpc44h[W6mIHnubIljcXS|IFPCJIFkfGm4aYT5 MXWyN|g5PzZyNR?=
KATO-II M2ruPGdzd3e2aDDpcohq[mm2b4L5JIF{e2G7 M1LkcVUh|ryP NXLTO3IzTE2VTx?= NV20RVdW[myxY3vzJJBzd2yrZnXyZZRqd25? NEG3SHozPTJ2OUW1Oy=>
OCUM-2M M{TReWdzd3e2aDDpcohq[mm2b4L5JIF{e2G7 NYTqXo5EPSEQvF2= M33ZcWROW09? MonTZoxw[2u|IIDyc4xq\mW{YYTpc44> NHv0TJEzPTJ2OUW1Oy=>
SNU-16 MWfHdo94fGhiaX7obYJqfG:{eTDhd5NigQ>? NX;LR2NKPSEQvF2= NXjPbWNkTE2VTx?= MXricI9kc3NicILvcIln\XKjdHnvci=> NHP1eWMzPTJ2OUW1Oy=>
HSC-39 M3fVd2dzd3e2aDDpcohq[mm2b4L5JIF{e2G7 NITETHE2KM7:TR?= MmeySG1UVw>? MV\icI9kc3NicILvcIln\XKjdHnvci=> NWq2[IF4OjV{NEm1OVc>
KATO-II M{PTWYN6fG:2b4jpZ4l1gSCjc4PhfS=> NI\aOJZ,OTBizszN NH3udFlFVVOR MkXKTWM2OD1yLkGgeI8hOi5yIN88cY9tN0x? NFj1[VYzPTJ2OUW1Oy=>
OCUM-2M M3;xdYN6fG:2b4jpZ4l1gSCjc4PhfS=> NVvlfmhPhjFyIN88US=> MWLEUXNQ NEjifmNKSzVyPUCuNUB1dyB{LkCg{txud2xxTB?= NH;jUpkzPTJ2OUW1Oy=>
SNU-16 MVXjfZRwfG:6aXPpeJkh[XO|YYm= MUL+NVAh|ryP NHuzelRFVVOR M1\jSmlEPTB;MD6xJJRwKDJwMDFOwI1wdC:O NHHrXWwzPTJ2OUW1Oy=>
HSC-39 Mm\XZ5l1d3SxeHnjbZR6KGG|c3H5 NIDzbGF,OTBizszN MUHEUXNQ MlH6TWM2OD1yLkGgeI8hOi5yIN88cY9tN0x? NY\ONVF2OjV{NEm1OVc>
NIH 3T3 MUXHdo94fGhiaX7obYJqfG:{eTDhd5NigQ>? Ml;TglExKM7:TR?= NHPydYxFVVOR MmjvbY5pcWKrdIOgZ4VtdCCpcn;3eIgh[W6mIHPvcI9vgSCob4LtZZRqd25? NFnKTXozPTJ2OUW1Oy=>
KATO-III NEnlZnJHfW6ldHnvckBie3OjeR?= MmfYNUDPxE1? M{DxSWROW09? MUTpcoR2[2W|IHPlcIwu[3mlbHWgZZJz\XO2 MX6yOVI1QTV3Nx?=
OCUM-2M NETUVW9HfW6ldHnvckBie3OjeR?= M3KyflEh|ryP NYDkTYt5TE2VTx?= MorSbY5lfWOnczDj[YxtNWO7Y3zlJIFzemW|dB?= NXX3cWxbOjV{NEm1OVc>
KATO-III NXHF[VhrSXCxcITvd4l{KGG|c3H5 NF\kPXAyKM7:TR?= Mmi0SG1UVw>? Mn3abY5lfWOnczDhdI9xfG:|aYO= M2rlO|I2OjR7NUW3
OCUM-2M MXTBdI9xfG:|aYOgZZN{[Xl? NHq3fXAyKM7:TR?= Mn:3SG1UVw>? MnvVbY5lfWOnczDhdI9xfG:|aYO= NGS1OIMzPTJ2OUW1Oy=>
KATO-III NEDTUmlHfW6ldHnvckBie3OjeR?= NEjuTJIyKM7:TR?= NYHYW5puTE2VTx?= NHjpRmhqdmirYnn0d{BHT0[UMjDwbI9{eGixconsZZRqd25iYX7kJIRwf26|dILlZY0he2mpbnHsbY5oKG2xbHXjeYxmew>? MoTRNlUzPDl3NUe=

... Click to View More Cell Line Experimental Data

In vivo The mice treated with 30 mg/kg or 100 mg/kg Pazopanib reveals a significant decrease in tumor burden compared with the mice treated with vehicle or 10 mg/kg Pazopanib. Treatment with Pazopanib is well-tolerated and there is no significant difference in the body weight among the mice in each group. [2]

Protocol

Kinase Assay:

[1]

+ Expand

Kinase enzyme assays:

VEGFR enzyme assays for VEGGR1, VEGFR2, and VEGFR3 are run in homogeneous time-resolved fluorescence (HTRF) format in 384-well microtiter plates using a purified, baculovirus-expressed glutathione-S-transferase (GST) fusion protein encoding the catalytic c-terminus of human VEGFR receptor kinases 1, 2, or 3. Reactions are initiated by the addition of 10 μL of activated VEGFR2 kinase solution [final concentration, 1 nM enzyme in 0.1 M HEPES, pH 7.5, containing 0.1 mg/mL bovine serum albumin (BSA), 300 μM dithiothreitol (DTT)] to 10 μL substrate solution [final concentration, 360 nM peptide, (biotin-aminohexyl-EEEEYFELVAKKKK-NH2), 75 μM ATP, 10 μM MgCl2], and 1 μL of titrated Pazopanib in DMSO. Plates are incubated at room temperature for 60 min, and then the reaction is quenched by the addition of 20 μL of 100 mM ethylene diamine tetraacetic acid (EDTA). After quenching, 20 μL HTRF reagents (final concentration, 15 nM Streptavidin-linked allophycocyanin, 1 nM Europium-labeled antiphosphotyrosine antibody diluted in 0.1 mg/mL BSA, 0.1 M HEPES, pH 7.5) is added and the plates incubated for a minimum of 10 min. The fluorescence at 665 nM is measured with a Wallac Victor plate reader using a time delay of 50 μs.
Cell Research:

[1]

+ Expand
  • Cell lines: HUVEC cells
  • Concentrations: 0-10 μM
  • Incubation Time: 1 hour
  • Method:

    Phosphorylation of VEGFR2 is assessed in HUVEC stimulated with VEGF. HUVEC are plated in type-I collagen-coated 10 cm plates in Clonetics EGM-MV medium at 1.0-1.5 × 106 cells/plate. After 24 hours, the confluent cells are serum starved overnight by replacing the growth medium with Clonetics EBM medium containing 0.1% BSA, 500 μg/mL hydrocortisone. Cells are treated with Pazopanib at various concentrations for 1 hour, followed by addition of 10 ng/mL VEGF or vehicle for 10 min. Cells are solubilized in lysis buffer. VEGFR2 is immunoprecipitated using antiflk-1 antibody and analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) followed by Western blotting and detection with antiflk-1 or with antiphosphotyrosine (anti-P-tyr-biotin) antibody. The VEGFR2 phosphorylation level is quantified by densitometry and normalized to the total VEGFR2 level.


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 17 mg/mL (35.86 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents individually and in order:
30% PEG400+0.5% Tween80+5% propylene glycol
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 473.98
Formula

C21H23N7O2S.HCl

CAS No. 635702-64-6
Storage powder
in solvent
Synonyms N/A

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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02300545 Recruiting Sarcoma, Soft Tissue|Soft Tissue Sarcoma Washington University School of Medicine April 8, 2015 Phase 2
NCT00674024 Completed Neoplasms|Lymphoma National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) October 7, 2008 Phase 1
NCT01716416 Recruiting Squamous Cell Carcinoma of the Head and Neck Washington University School of Medicine May 31, 2013 Phase 1
NCT01462630 Recruiting Adult Angiosarcoma|Recurrent Adult Soft Tissue Sarcoma|Stage III Adult Soft Tissue Sarcoma|Stage IV Adult Soft Tissue Sarcoma Fox Chase Cancer Center|National Cancer Institute (NCI) November 3, 2011 Phase 2
NCT01468922 Completed Sarcoma|Stomach Neoplasms|Neoplasms National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) October 24, 2011 Phase 1
NCT02342600 Not yet recruiting Gastrointestinal Stromal Tumors Sarcoma Alliance for Research through Collaboration|Novartis January 2017 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID