Gefitinib (ZD1839)

Catalog No.S1025

Gefitinib (ZD1839) Chemical Structure

Molecular Weight(MW): 446.90

Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.

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Cited by 92 Publications

13 Customer Reviews

  • Perturbation of EGFR by its ligand EGF and gefitinib (ZD-1839 Iressat; inhibits EGFR) produces opposite responses in the predicted EGFR target genes SOCS2 and NR2E1.

    Mol Syst Biol 2011 7, 486. Gefitinib (ZD1839) purchased from Selleck.

    Effects of shRNA-mediated RECK depletion on EGFR signaling in MEFs. (a) Immunoblotting (IB) of the indicated proteins in wild-type MEFs transduced with the indicated shRNAs and cultured for 48 h in the presence of 0.1% dimethyl sulfoxide (DMSO; vehicle) or 1 mM gefitinib (S1025, Selleck Chemicals,Houston, TX, USA). (b) Immunoprecipitation from whole lysates of cells from panel a with an anti-phosphorylated tyrosine (pTyr) antibody. Precipitates from 500 mg protein in whole cell lysates (upper) or 20 mg protein in whole cell lysates (lower) were analyzed by IB with anti-EGFR antibody.

    Oncogene 2010 30, 737-750. Gefitinib (ZD1839) purchased from Selleck.

  • (B–C) LNCaP (B) and LNCaP-AI (C) cells were transiently transfected with sPLA2-IIa(-800)-Luc (0.5 μg). The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 lM) and Bortezomib (20 μM) without or with EGF (100 ng/ml) for 24 h. Luciferase assay was performed according to a standard protocol with Renilla luciferase as an internal control. Data are presented as the mean (±SD) of duplicate values of a representative experiment that was independently repeated for five times.

    Carcinogenesis 2010 31, 1948–1955 . Gefitinib (ZD1839) purchased from Selleck.

    LNCaP-AI cells were starved in 1% stripped medium for 24 h. The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 μM) and Bortezomib (20 μM) for 24 h. Cell culture medium was collected from each sample and subjected to ELISA for sPLA2-IIa. The condition medium samples were diluted 10 times for ELISA. Average of duplicate samples was converted to nanogram per milliliter against standard curve. The data represent one of five repeated experiments.

     

     

    Carcinogenesis 2010 31, 1948–1955. Gefitinib (ZD1839) purchased from Selleck.

  • Susceptibility of lung cancer cells to cytolytic activity of NK-92 cells after treatment with EGFR inhibitors. Three lung cancer cells were untreated (open circle) or treated with 10 μM erlotinib or gefitinib (black filled square or black filled triangle) for 24 hours. The lung cancer cells were cocultured with NK-92 cells at indicated effector to target ratio (E:T ratio). To determine the specificity of NKG2D-mediated cytolysis of lung cancer cells, NK-92 cells were preincubated with blocking mAb against NKG2D before the assay (gray filled square and triangle) and correspond-ing isotype mAb (open square and triangle).

    J Immunother 2011 34(4), 372-81. Gefitinib (ZD1839) purchased from Selleck.

    Suppression of EGFR signaling by Gefitinib is dose dependent and non- toxic at millimolar concentrations.Results from plaque reduction tests of Hep2 cells infected with VACV(12.5pfu/well) treated with a serial dilution of Gefitinib (1000-0.01 m) and analyzed for (A)proliferation as indicator of cytotoxicity,(B) IC50 of plaque size inhibition, (C)dose dependent inhibition of EGFR-ERK1/2signaling and VACV proteins by Western blot analysis (pEGFR detection was obtained from a different experiment with identicals ettings) and (D)dose dependent inhibition of orthopoxvirus genome replication by real-time PCR.Untreated VACV infectedcells and uninfected cells are shown as controls in the right panel.

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

  • A circular zone of EGFR activated but uninfected cells facilitate virus induced plaque development , efficiently blocked by Gefitinib. (A)Immun of luorescent staining of aplaque reduction tests with Hep2 cells 24,48,72 and 96 h after VACV infection (6.25pfu/chamber). VACV infected cells(green) were visualized by human IgG directed to orthopoxviruses (VIG) detected with a secondary FITC labelled antibody. Uninfected cells with activated EGFR signaling(red) are detected with a secondary Cy3labelled antibody. Positive cells for both , VACV and phosphorylated EGFR are shown in yellow(merge). Cellular nuclei are counterstained with DAPI. (B)Immunofluorescent staining of a plaque reduction test as described for (A) and treated with three different Gefitinib concentrations (1000 nM, 1 nM, and 0.01 nM) . The test was analyzed 96h afterVACV infection.Uninfected cells are shown as controls. Magnification100×.(For interpretation of the references to color in this figure legend , the reader is referred to the web version of the article.)

     

     

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

    The EGF-dependent increased association of PCM1 and AZI1 requires EGFR kinase activity. ZD1839 (Iressa)—EGFR tyrosine kinase inhibitor.

    Mol Biosyst 2011 7, 3223-33. Gefitinib (ZD1839) purchased from Selleck.

  • Inhibition of 5-HT-induced 5-HT3A and 5-HT3AB receptor currents.

    Eur J Pharmacol, 2017, 821:21-28. Gefitinib (ZD1839) purchased from Selleck.

    Reduction of GPER expression in HCC1806 cells after treatment with gefitinib. (A) Concentration dependence. HCC1806 cells were treated with 50-500 nM gefitinib for 48 h. GPER was detected by western blotting. (B) Time dependence. HCC1806 cells were treated with 200 nM gefitinib for 24-96 h. ap<0.05 vs. control; bp<0.001 vs. control. Mean values and SE of three independent experiments are shown. (C) Representative western blotting of concentration dependence. (D) Representative western blotting of time dependence

    Oncol Rep, 2017, 37(2):1212-1218. Gefitinib (ZD1839) purchased from Selleck.

  • Inhibition of signaling pathway activation in lung tumor cell lines by kinase inhibitors. Lung tumor cells were cultured in 10% FBS until reaching ∼80% confluence and then the cells were starved in serum-free medium for overnight, followed by 4-hour treatment with the inhibitors. Cell lysates were then prepared and used for determination of the pathway activation signals by the CEER assay.

    Int J Proteomics 2011 215496. Gefitinib (ZD1839) purchased from Selleck.

    Breast cancer cells were pretreated with 100ng/ml EGF for 15 min and then treated with the indicated concentrations of  Gefitinib for 24 hours.

     

     

    2010 Dr. Zhang of Tianjin Medical University. Gefitinib (ZD1839) purchased from Selleck.

  • MTT assay result. 0-500nM Gefitinib treated with H1299, H358, H25 and HCC827 cell lines by 72h, Proliferation rate was tested.

     

     

    2010 Dr. Vicky Tin from University of Hong Kong. Gefitinib (ZD1839) purchased from Selleck.

Purity & Quality Control

Choose Selective EGFR Inhibitors

Biological Activity

Description Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.
Features A potent EGFR tyrosine kinase inhibitor.
Targets
Tyr1173 (NR6W cells) [1] Tyr1173 (NR6wtEGFR cells) [1] Tyr992 (NR6wtEGFR cells) [1] Tyr992 (NR6W cells) [1]
26 nM 37 nM 37 nM 57 nM
In vitro

Gefitinib effectively inhibits all tyrosine phosphorylation sites on EGFR in both the high and low-EGFR-expressing cell lines including NR6, NR6M and NR6W cell lines. The phosphorylation sites Tyr1173 and Tyr992 are less sensitive requiring higher concentrations of Gefitinib for inhibition. Gefitinib effectively blocks the phosphorylation of PLC-γ, with IC50 of 27nM, in NR6W cells. The NR6wtEGFR and NR6M cell lines has low levels of PLC-γ phosphorylation but the level in the NR6M cell line is more resistant to inhibition by Gefitinib with IC50 of 43 nM and 369 nM, respectively. Gefitinib inhibits Akt phosphorylations, with IC50 of 220 and 263nM, in the low-EGFR- and -EGFRvIII-expressing cell lines, respectively. Gefitinib in the dose range from 0.1 to 0.5μM significantly facilitates, rather than abrogates, colony formation of NR6M cells. However, at a concentration of 2 μM Gefitinib completely blocks NR6M colony formation. Gefitinib rapidly and in a dose-dependent manner inhibits EGFR and ERK phosphorylation up to 72 hours after EGF stimulation in both the high- and low-EGFR-expressing cell lines. [1] Gefitinib is the monolayer growth of these EGF-driven untransformed MCF10A cells with an IC50 of 20 nM. [2] The combination of Gefitinib (0.2 μM and 0.5 μM) with irradiation lead to a significant growth inhibition in LoVo cells, compared with radiation alone.. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KB NFzTN4pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUDJR|UxRTBwMEW0JO69VQ>? NFfzZVYyPjVzNkS3Ny=>
NIH3T3 MXHGeY5kfGmxbjDBd5NigQ>? MlnVNE4zKM7:TR?= M2LFdlIhcA>? NEe4[WdFVVOR M4LFdmlvcGmkaYTpc44hd2ZiRVfGJJN1cW23bHH0[YQhcHWvYX6g[ZJjSjFiYYX0c5Bpd3OyaH;yfYxifGmxbjD3bZRpKEmFNUCgc4YhOC5yMUS0JO69VQ>? Mmr6NVY1QDB{OES=
COS7 MnTpSpVv[3Srb36gRZN{[Xl? NUSzR3lQQTZizszN M4\CZmlvcGmkaYTpc44hd2ZiaIXtZY4hUkGNMjDWOlE4TiCvdYThcpQh\XiycnXzd4Vl NVXk[Yx6OTdzN{i3NlI>
A549 MU\DfZRwfG:6aXOgRZN{[Xl? NWDSNHlUQTZiaB?= NHf2SY9FVVOR NFvzSGhKSzVyPUGzMlU6KM7:TR?= MUixPFMyOzhyNx?=
K562 NUj3[oM6S3m2b4TvfIlkKEG|c3H5 M2juNVk3KGh? MlnsSG1UVw>? NIrNToNKSzVyPUmuN|Yh|ryP M3S1dlE5OzF|OEC3
MDA-MB-231 M4\XT2N6fG:2b4jpZ{BCe3OjeR?= NHL5eI06PiCq NIrRPGVFVVOR NF\QZ5VKSzVyPUO2MlYyKM7:TR?= NUTQbnh6OTh|MUO4NFc>
MOLT4 NH7xfopEgXSxdH;4bYMhSXO|YYm= MmWyPVYhcA>? MoTuSG1UVw>? MVXJR|UxRTF3LkCyJO69VQ>? MlPGNVg{OTN6MEe=
MCF7 M2L6UmN6fG:2b4jpZ{BCe3OjeR?= MUe1NEDPxE1? MkXJO|IhcA>? NFfafI1KSzVyPUGyMlA2KM7:TR?= M1P5UFE5PzdzOEG5
BT474 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUnOd29QPzJiaB?= Ml75RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCEVES3OEBk\WyuczDveoVz\XiycnXzd4lv\yCHUlLiNkB4cXSqIFXDOVAhd2ZiMD6zJO69VQ>? MlXPNVkxOjh2MkS=
HN5 MmXPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M33w[lczKGh? NHz4VFFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjOOUBk\WyuczDveoVz\XiycnXzd4lv\yCHR1\SJJdqfGhiRVO1NEBw\iByLkCxJO69VQ>? MlnTNVkxOjh2MkS=
BA/F3 MYHDfZRwfG:6aXOgRZN{[Xl? MmG2O|IhcA>? NELzNYhEgXSxdH;4bYNqfHliYXfhbY5{fCCvb4Xz[UBDSS:IMzDj[YxteyCneIDy[ZN{cW6pIFXHSnIhVDh3OGKgcZV1[W62IIfpeIghUUN3MDDv[kAxNjBzMEig{txO MWSxPVI{QTJ{OR?=
NIH3T3 Ml;aS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV:3NkBp NITU[3hIem:5dHigbY5pcWKrdHnvckBw\iCvb4Xz[UBPUUhxM2SzJINmdGy|IHX4dJJme3Orbneg[ZJDOiCpZX7lJJdqfGhiSVO1NEBw\iByLkW0JO69VQ>? NYXPS25JOTl4NkWzO|c>
MCF7 MX Mlq4SpVv[3Srb36gRZN{[Xl? MWKzNEBucW5? NHT1[XdKdmirYnn0bY9vKG:oIFLDVnAh\XiycnXzd4VlKHerdHigTWM2OCCxZjCxMlEh|ryP NVLLTItFOTl7M{K5OlA>
A431 MkL5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXqxNEDPxE1? M2fSflczKGh? MlLTS2k2OD1yLkWxOEDPxE1? MVuxPVk3QTR4NR?=
A431 NU\VU|YxU2mwYYPlJGF{e2G7 NEP4cGkxNjFizszN NYDGPY1JOSCq NIDoVnBKdmirYnn0bY9vKG:oIFXHSk1qdmS3Y3XkJGVITlJicHjvd5Bpd3K7bHH0bY9v NILrZXQyQTl4OUS2OS=>
A431 M{fHfGtqdmG|ZTDBd5NigQ>? M2PE[FExOCEQvF2= MXqxNEBucW5? MnnpVJJwdG:wZ3XkJIlvcGmkaYTvdpkh[WO2aY\peJkhd2ZiRVfGMYlv\HWlZXSgSWdHWiCyaH;zdIhwenmuYYTpc44> NGfjN2EyQTl4OUS2OS=>
Sf9 NG\XSHpMcW6jc3WgRZN{[Xl? NEjqR2IyOCCvaX6= MnfLSG1UVw>? MWjJcohq[mm2aX;uJI9nKEWJRmKg[ZhxemW|c3XkJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gdoVk\XC2b4KgZZV1d3Cqb4PwbI9zgWyjdHnvckB4cXSqIFnDOVAhd2ZiMD6wN|Mh|ryP MX[yNFA2PjR{NR?=
NCI-H1975 Mm\aS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NED2fpI2KM7:TR?= NV\nOVhWPzJiaB?= NF;QUGtKSzVyPUiuNlYh|ryP NWDOVXdzOjBzNUG2O|A>
NCI-H1975 M{DtUGtqdmG|ZTDBd5NigQ>? NIfFSXcyOCEQvF2= NEHPeHFKdmirYnn0bY9vKG:oIFXHSnIh[XW2b4Doc5NxcG:{eXzheIlwdg>? MYGyNFE2OTZ5MB?=
NCI-H1975 NGraWZJHfW6ldHnvckBCe3OjeR?= MWmxNEDPxE1? MUDJcohq[mm2aX;uJI9nKEWUQlKyJIF2fG:yaH;zdIhwenmuYYTpc44> MWGyNFE2OTZ5MB?=
NCI-H1975 M1K2OmZ2dmO2aX;uJGF{e2G7 M17QXVUh|ryP NXjaT3VMPDhiaB?= MVzJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGG|c3Xzd4VlKGG|IHHjeIl3[XSrb36gc4Yh[2G|cHHz[U0{ M{\QOlIxOTVzNkew
A375 MkmyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3rRNVQ5KGh? MYXJR|UxRTJyLkW1JO69VQ>? NFT4Uo0zODNyNEWzOy=>
SMMC7721 MlPyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFnzV2k1QCCq M{fTbWlEPTB;M{OuNVIh|ryP M3;KNFIxOzB2NUO3
DU145 MVvDfZRwfG:6aXOgRZN{[Xl? M2rMeVUh\A>? NFPtUYpFVVOR MVjJR|UxRTR{LkG3JO69VQ>? M1KzdVIxPDZ4NUW1
MIAPaCa2 NVzlSFM2S3m2b4TvfIlkKEG|c3H5 M1\POlUh\A>? MWrEUXNQ NFO3WYVKSzVyPUWwMlYzKM7:TR?= NX;WcGU1OjB2Nk[1OVU>
PANC1 Mkf6R5l1d3SxeHnjJGF{e2G7 MofjOUBl MWDEUXNQ NWf1fFlbUUN3ME20NE41OiEQvF2= MXiyNFQ3PjV3NR?=
PC9 NW\UfJBbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn;GRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDFS2ZTNWSnZnnjbYVvfCCqdX3hckBRSzliY3XscJMhf2m2aDDFR|UxKG:oIECuNFE4KM7:TR?= NGPINHgzOTJyOEiwNi=>
PC9 NFvIUpNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEG2fVBCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIdzd3e2aD3y[ZNqe3SjboSgbJVu[W5iUFO5JINmdGy|IHX4dJJme3OrbnegSWdHWiCHN{S2Y2E4PTBxVEe5NG0hdXW2YX70JJdqfGhiRVO1NEBw\iB5IN88US=> M{LmVlIyOjB6OECy
PC3 NEntc3ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUexNEDPxE1? NGXTOZg4OiCq M2flOGROW09? M1vVXWlEPTB;Nz60JO69VQ>? MmrZNlE{PTN3NE[=
MDA-MB-468 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHHtTVgyODBizszN Mn;FOFghcA>? MmW2SG1UVw>? MkTyRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdIm= MX:yNVQzQTZ|Mh?=
Plasmodium falciparum NEPLZXRyUFSVIIDyc4ZqdGmwZzDmc5IhcW6qaXLpeI9zeyCxZjDQcIF{dW:maYXtJIZidGOrcHHyeY0heHKxbHnm[ZJifGmxbh?= MWKyNVgyPzB2NR?=
NCI-H1993 NGSyPZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWi3NkBp MlfFSG1UVw>? MXnJR|UxRTBwMTFOwG0> NHn1PYozOTlzNkSzNy=>
NCI-H292 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkWyO|IhcA>? NIrJcFFFVVOR NV36V21uUUN3ME2wMlAzKM7:TR?= NWrS[FkzOjF7MU[0N|M>
A431 M3jFT2tqdmG|ZTDBd5NigQ>? NXm3d4QxOiCq M{SybmROW09? NX7MZ4lSUW6qaXLpeIlwdiCxZjDFS2ZTKHCqb4PwbI9zgWyjdHnvckBqdiCHR1[td5RqdXWuYYTl[EBpfW2jbjDBOFMyKHerdHigTWM2OCCxZjCwMlAxOSEQvF2= NVnwbIJKOjJzMEGxN|I>
MCF7 NHnrZ2tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUexNFDDqM7:Zz;tUC=> M4e2WFQ5KGh? MorFSG1UVw>? NXjQbZBmSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJI93\XKneIDy[ZN{cW6pIFXHSnIh\2WwZTD3bZRpKEmFNUCgc4YhOTJwMEWg{txO NIqyc4ozOjFzOUGzNC=>
BT474 M{Tpe2N6fG:2b4jpZ{BCe3OjeR?= NInDdVIyOCEQvF2= M2DqflQ5KGh? NX\VUYdvS3m2b4TvfIlkcXS7IHHnZYlve3Ric3XyeY0ue3Sjco\l[EBpfW2jbjDCWFQ4PCClZXzsdy=> Mn3MNlIyOzh|MEi=
MCF7 MVXDfZRwfG:6aXOgRZN{[Xl? MVuxNEDPxE1? NGjvO2w1QCCq NUXHTm5YS3m2b4TvfIlkcXS7IHHnZYlve3Ric3XyeY0ue3Sjco\l[EBpfW2jbjDNR2Y4KGOnbHzzJI93\XJvZYjwdoV{e2mwZzDFdoJDOiClZXzsdy=> Mn3LNlIyOzh|MEi=
HCC827 NXjNN3ZDU2mwYYPlJGF{e2G7 NX7XNFkzOC5zMkWg{txO M33DTlI1KGh? MUHEUXNQ M4rLS2lvcGmkaYTpc44hd2ZiRVfGVkBl\WxiRUe0Ok1CPzVyIH31eIFvfCC\MUC2PEBifXSxcHjvd5Bpd3K7bHH0bY9v MnPZNlI{Ozl|NEK=
HCC827 MULLbY5ie2ViQYPzZZk> MVGwMlEzPSEQvF2= MmDjNlQhcA>? NYW2T5p2TE2VTx?= NFr0N5dKdmirYnn0bY9vKG:oIFXHSnIh\GWuIFW3OFYuSTd3MDDteZRidnRvbXXkbYF1\WRiRYLrJJBpd3OyaH;yfYxifGmxbh?= M4LGVVIzOzN7M{Sy
HCC827 NVT6d3J{U2mwYYPlJGF{e2G7 MlnlNE4yOjVizszN M3mwdVI1KGh? NV\pNoxpTE2VTx?= NFy3coJKdmirYnn0bY9vKG:oIFXHSnIh\GWuIFW3OFYuSTd3MDDteZRidnRvbXXkbYF1\WRiQXv0JJBpd3OyaH;yfYxifGmxbh?= NFrMbHczOjN|OUO0Ni=>
A431 NGDTVmNMcW6jc3WgRZN{[Xl? MUmxNFAh|ryP Mm\jN|AhdWmw NWfmdpFrUW6qaXLpeIlwdiCxZjDFS2ZTKGSncnn2[YQh\nKxbTDoeY1idiCDNEOxJINmdGxibXXtZpJidmViYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCyb3z5MWdtfS2WeYKgdIhwe3Cqb4L5cIF1cW:wIIfpeIghUUN3MDDv[kAyNjB6IN88US=> MkXUNlI3QTh5OEK=
H460 MnOxR5l1d3SxeHnjJGF{e2G7 MWK3NkBp MlK1TWM2OD13LkW5JO69VQ>? MmfwNlI4Ojd2NEm=
HT-29 MYTDfZRwfG:6aXOgRZN{[Xl? MnTFO|IhcA>? NGPJRo5KSzVyPUOuN|Yh|ryP Mnv6NlI4Ojd2NEm=
SGC7901 NEK2[YhEgXSxdH;4bYMhSXO|YYm= Mlm2O|IhcA>? MW\JR|UxRTFyLkK2JO69VQ>? M3O1SVIzPzJ5NES5
A498 M2PMSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NITrRpNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFG0PVgh[2WubIOg[ZhxemW|c3nu[{BGT0[UIHHu[EBUWkNid3n0bEBKSzVyIH;mJFgvPiEQvF2= MYKyNlgyQDh2OB?=
Calu3 NH\G[|NEgXSxdH;4bYMhSXO|YYm= NGXESoUyOCEQvHevcWw> MnTuO|IhcA>? NG\aRW5KSzVyPUGuOkDPxE1? Mmn3NlMyOTZzNki=
FADU MVfDfZRwfG:6aXOgRZN{[Xl? NFnKVoQyOCEQvHevcWw> M{C0eVczKGh? MljNTWM2OD1yLkC1JO69VQ>? MXqyN|EyPjF4OB?=
HCC827 MVnDfZRwfG:6aXOgRZN{[Xl? M2CwRlExKM7:Zz;tUC=> MlXGO|IhcA>? M{e2bWlEPTB;MD6wNFE2KM7:TR?= MoL0NlMyOTZzNki=
PC9 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1m3dlExKM7:Zz;tUC=> MXG3NkBp Mln6TWM2OD1yLkCwNVQh|ryP MXWyN|EyPjF4OB?=
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IGROV-1 M4XaOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkT3TWM2OD1{LkOxNlM4KM7:TR?= NF:3T2JUSU6JRWK=
JVM-3 NVGwb5hQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWDQfFd[UUN3ME2yMlM1PjR|IN88US=> NEHDW5lUSU6JRWK=
NCI-H2170 MmPmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEfpXnJKSzVyPUKuN|Q4QDNizszN NV\YblgyW0GQR1XS
EW-18 NU[ycmxlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1X1UmlEPTB;Mj6zOVQ4OSEQvF2= NWC1[3pRW0GQR1XS
KU812 M4D0N2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3rUbWlEPTB;Mj6zOlc4QSEQvF2= NU\BfYNwW0GQR1XS
NY MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NG[5TopKSzVyPUKuN|czPyEQvF2= NYPh[HV6W0GQR1XS
HTC-C3 NWHJT3IxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWLJR|UxRTJwNEKyOlch|ryP MlixV2FPT0WU
CFPAC-1 NVjVOJJwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn;KTWM2OD1{LkSzPFY1KM7:TR?= MUTTRW5ITVJ?
HH NV\kVZNWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoXVTWM2OD1{LkS5PFU1KM7:TR?= NFT0ZndUSU6JRWK=
MKN7 NIrvT3JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MY\JR|UxRTJwNkK3OFgh|ryP NELiUmtUSU6JRWK=
TE-8 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn6wTWM2OD1{Lk[zOlU{KM7:TR?= NH7ubIlUSU6JRWK=
MC-IXC MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1H2[GlEPTB;Mj62OFQ2OyEQvF2= MX\TRW5ITVJ?
DMS-79 NFO2e5VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV\1eYdtUUN3ME2yMlY6Ojh2IN88US=> MoXOV2FPT0WU
Detroit562 Mo\tS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4rQOGlEPTB;Mj62PVg1PSEQvF2= M3rCeXNCVkeHUh?=
HuP-T4 Mn;pS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHzrWVZKSzVyPUKuO|I5OThizszN NETRcIZUSU6JRWK=
QIMR-WIL NVS4cVd3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUXJR|UxRTJwN{W5OVIh|ryP MlvZV2FPT0WU
BB65-RCC NYj2dYFKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF3hXZNKSzVyPUKuPFA4OTNizszN M134[3NCVkeHUh?=
PC-3 M2LhRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUnnVJFMUUN3ME2yMlg{OzF5IN88US=> MmHUV2FPT0WU
OVCAR-3 NFLnd45Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV7ic|J[UUN3ME2yMlg2PTl2IN88US=> MVPTRW5ITVJ?
KYSE-510 NYHsU4hpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoPzTWM2OD1{Lki2O|g3KM7:TR?= MmfTV2FPT0WU
HC-1 MknYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYfJR|UxRTJwOUSwNVIh|ryP NFTmdphUSU6JRWK=
NCI-H1693 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVj4WmFEUUN3ME2yMlk2QDl5IN88US=> M3rKUXNCVkeHUh?=
DU-4475 NH34UFRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEHOPYhKSzVyPUKuPVkxPTJizszN MmHEV2FPT0WU
COLO-680N MofIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGPZc49KSzVyPUOuNFA1QTVizszN NGD4[mZUSU6JRWK=
MN-60 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3i5VGlEPTB;Mz6wOFk4PCEQvF2= NHTEVJRUSU6JRWK=
BCPAP NU\WUG9OT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVfJR|UxRTNwMEiyPVUh|ryP MYXTRW5ITVJ?
647-V NGDVZoxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXP4TWlIUUN3ME2zMlE3OjJizszN NXL2RW5{W0GQR1XS
HT-1376 NILYfGdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHz5ZYRKSzVyPUOuNlA2ODZizszN NHHCO|VUSU6JRWK=
NCI-H1793 NF\ZS4tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIfU[WZKSzVyPUOuNlExQThizszN MVvTRW5ITVJ?
SW1463 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnX0TWM2OD1|LkK2NVkzKM7:TR?= M4HuVnNCVkeHUh?=
NCI-H727 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHTsZohKSzVyPUOuN|g2QDRizszN NEDVeoVUSU6JRWK=
LB771-HNC MmTvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEPhbVJKSzVyPUOuN|kyOjZizszN M1z4THNCVkeHUh?=
D-542MG M{nUXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFzUU|BKSzVyPUOuOFQzOjJizszN NF3YTJNUSU6JRWK=
NTERA-S-cl-D1 MnznS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3rOXWlEPTB;Mz60OFk2PyEQvF2= MWTTRW5ITVJ?
C8166 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVHuZ3QyUUN3ME2zMlQ3PTJzIN88US=> NXfKOlZDW0GQR1XS
639-V M{Htcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlXsTWM2OD1|LkW3O|kzKM7:TR?= NX7EcFNpW0GQR1XS
TE-1 MkD1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXnJR|UxRTNwNUmxOlkh|ryP MYfTRW5ITVJ?
OAW-28 MmfqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3HiVGlEPTB;Mz61PVQzPiEQvF2= MoP5V2FPT0WU
SK-LU-1 M{H2T2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVi0WlM5UUN3ME2zMlYxPjR3IN88US=> MUHTRW5ITVJ?
CaR-1 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVroTW9DUUN3ME2zMlYyODR6IN88US=> M2\RdnNCVkeHUh?=
A2780 NF\u[XVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2faOGlEPTB;Mz62OFA5QSEQvF2= NHj4ZoNUSU6JRWK=
C2BBe1 MoDJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVvJR|UxRTNwNkS0NFch|ryP NYj1cFZXW0GQR1XS
SK-MES-1 M4nRXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkK5TWM2OD1|Lk[0OlE1KM7:TR?= M{jnfHNCVkeHUh?=
PANC-10-05 NGL3UXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXHJR|UxRTNwN{KwO|Ih|ryP M37XfnNCVkeHUh?=
J-RT3-T3-5 MlXlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIfSOnpKSzVyPUOuO|U1ODZizszN MX7TRW5ITVJ?
ES4 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYfJR|UxRTNwOEKyPFYh|ryP NEjCZmdUSU6JRWK=
NCI-H28 NX3CUnQ5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVfJR|UxRTNwOEK5OlEh|ryP NWjrfopyW0GQR1XS
NCI-H1155 NF[weW9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVPJR|UxRTNwOESxOVkh|ryP NXKxToFLW0GQR1XS
KURAMOCHI M2TlXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnrkTWM2OD1|Lki2NFU3KM7:TR?= MoLSV2FPT0WU
NCI-H69 M4n5S2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnTxTWM2OD1|LkmwNVc5KM7:TR?= M13CW3NCVkeHUh?=
IST-MES1 MoTrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYP4SoYyUUN3ME2zMlk1ODJ7IN88US=> NXzoWIVPW0GQR1XS
NH-12 M2[xSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXfJR|UxRTNwOUSxPFch|ryP MnntV2FPT0WU
JVM-2 MlnhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4rHcWlEPTB;Mz65OFc2OiEQvF2= MknlV2FPT0WU
EW-16 MmLtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGH6XGNKSzVyPUOuPVU2PzdizszN M2jDPHNCVkeHUh?=
NCI-H441 MnPnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1XLVGlEPTB;Mz65O|AyPCEQvF2= NWPVeHFDW0GQR1XS
RCC10RGB MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4\0NWlEPTB;ND6wNVI1PiEQvF2= MoTYV2FPT0WU
TCCSUP MoLwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYXJR|UxRTRwMUGwOUDPxE1? NInJUJBUSU6JRWK=
HL-60 NILTT5JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{\TPWlEPTB;ND6xNlI1OSEQvF2= MoDuV2FPT0WU
EW-11 M2fNe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUTCcYhCUUN3ME20MlE{OjZzIN88US=> MUfTRW5ITVJ?
KARPAS-299 M3nDWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUfJR|UxRTRwM{GwNVMh|ryP NFjDcppUSU6JRWK=
SNU-C2B NIXZR21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGmyRoRKSzVyPUSuN|Q3QDZizszN MnP2V2FPT0WU
LU-139 NXXDW|ZbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWjJR|UxRTRwM{eyOFch|ryP MXLTRW5ITVJ?
HCC1937 MkHUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3jEVWlEPTB;ND6zO|k6OyEQvF2= M4HOOXNCVkeHUh?=
GR-ST M2PxN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4XpXGlEPTB;ND60N|E5KM7:TR?= MnrYV2FPT0WU
SK-OV-3 NFq5ToRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV3JR|UxRTRwNE[wOFIh|ryP NUnvXJFyW0GQR1XS
A172 NIrCWHFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWHJR|UxRTRwNUW1NlEh|ryP NYLpU4ViW0GQR1XS
NB10 Ml2xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkXpTWM2OD12LkW5O|gyKM7:TR?= M2WzR3NCVkeHUh?=
P30-OHK NUD6Oo0xT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYHE[YVHUUN3ME20MlgyQTJ7IN88US=> MlTaV2FPT0WU
KYSE-520 NFW0c5lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MljmTWM2OD12Lki4NFE5KM7:TR?= MV\TRW5ITVJ?
KNS-62 NVTmcWxzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFXrW5hKSzVyPUSuPVA5ODdizszN MUnTRW5ITVJ?
ES1 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NG\QcVVKSzVyPUSuPVYxOzVizszN NUHMNot5W0GQR1XS
KOSC-2 NHPS[GpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYHnbWdHUUN3ME20Mlk6QTV7IN88US=> Mn3YV2FPT0WU
TE-9 NFTsR4dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4j5dmlEPTB;NT6wNVUzPSEQvF2= NGLzfFlUSU6JRWK=
NCI-H1838 NEO0ZZJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3HHNmlEPTB;NT6wNlA4PiEQvF2= MUnTRW5ITVJ?
KP-N-YN NEnLRXZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4D6PGlEPTB;NT6xNlU6OSEQvF2= NWDMSVZHW0GQR1XS
ALL-PO NEfLbGRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2q5SGlEPTB;NT6yNlU5PyEQvF2= MmLrV2FPT0WU
NOMO-1 MoHJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHHhd4VKSzVyPUWuNlMyPzZizszN M3;0XHNCVkeHUh?=
CGTH-W-1 NVHjeFhsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mom1TWM2OD13LkO4OVg1KM7:TR?= NHzOVpdUSU6JRWK=
ATN-1 MmXkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV7JVpdmUUN3ME21MlQyOTh2IN88US=> NEnwN3NUSU6JRWK=
AGS M37teWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2W5TWlEPTB;NT60PVYyQCEQvF2= NEDre5RUSU6JRWK=
SW13 NFPvNZNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFfzSmtKSzVyPUWuOVMzPjVizszN MlXpV2FPT0WU
SW1990 M2jCWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4HVUGlEPTB;NT62NlI5PSEQvF2= NWHadZRKW0GQR1XS
BFTC-905 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFHwRZhKSzVyPUWuOlU5PzhizszN NIH0[VFUSU6JRWK=
RMG-I MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXG1fJZiUUN3ME21MlY2QTR{IN88US=> NG\yZpRUSU6JRWK=
HD-MY-Z MlP3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVHJR|UxRTVwNki1PFEh|ryP MoDDV2FPT0WU
HCC1187 M1vmWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF3FRY1KSzVyPUWuO|ExOzJizszN MY\TRW5ITVJ?
HCC2998 NXvYSXFrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUnJR|UxRTVwN{G4O|ch|ryP MXfTRW5ITVJ?
RT-112 M4jBcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX;JR|UxRTVwOUCyOFUh|ryP MnfKV2FPT0WU
CAKI-1 M{XUPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MojTTWM2OD14LkCwPVg2KM7:TR?= MV7TRW5ITVJ?
HDLM-2 NH2zcVJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIP3RmFKSzVyPU[uNFE1QDlizszN MlHPV2FPT0WU
TE-10 MoLLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXnJR|UxRTZwMES4N|Mh|ryP MUnTRW5ITVJ?
KE-37 NF3PfVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlnUTWM2OD14LkC3NlU5KM7:TR?= MoTIV2FPT0WU
RCM-1 NHLxOI1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHrFPVJKSzVyPU[uNFc4OTlizszN MUDTRW5ITVJ?
Mewo MnvSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MW\JR|UxRTZwMUG0PFch|ryP MmrnV2FPT0WU
L-428 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MW\JR|UxRTZwMUiwN|ch|ryP MX3TRW5ITVJ?
8-MG-BA M2mwNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUTJR|UxRTZwMUmzO|kh|ryP NX\0fZZqW0GQR1XS
SK-MEL-28 NX[wbIQxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFX3[GpKSzVyPU[uNlA6PjdizszN M3vUeXNCVkeHUh?=
VA-ES-BJ NHfCOpFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{nUVGlEPTB;Nj6zNFQ3OiEQvF2= MUPTRW5ITVJ?
CAL-120 MontS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MULJR|UxRTZwNEWxOlIh|ryP NHTUfZBUSU6JRWK=
GAMG MlW4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWLJR|UxRTZwNUC2NkDPxE1? M1HOUHNCVkeHUh?=
MSTO-221H M37vcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mle4TWM2OD14LkWxNVch|ryP NFzIUmtUSU6JRWK=
GCT NWLoOo9nT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYf4NpVRUUN3ME22MlU{Ozh5IN88US=> MX\TRW5ITVJ?
SH-4 M36xZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn6zTWM2OD14LkW4PFQ1KM7:TR?= NE\qc|FUSU6JRWK=
Calu-3 M1HITWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX7JR|UxRTZwNkW1Olgh|ryP MnnDV2FPT0WU
SK-HEP-1 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX7JR|UxRTZwN{G3OVMh|ryP MVfTRW5ITVJ?
PFSK-1 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYPJR|UxRTZwN{O0O|gh|ryP NFrweHNUSU6JRWK=
NB12 M1LWVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2e4W2lEPTB;Nj63PFQxPCEQvF2= MlT6V2FPT0WU
A388 NGT2bppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXjDUXJLUUN3ME22Mlg6PTJizszN MWHTRW5ITVJ?
KLE MoDXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVjJR|UxRTZwOU[1NVYh|ryP M1nhfnNCVkeHUh?=
TE-11 M4TtU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4nRSWlEPTB;Nz6wOFk3PyEQvF2= NWn4cJhoW0GQR1XS
NCI-H2405 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGDK[3hKSzVyPUeuNFg{QTNizszN MVjTRW5ITVJ?
OVCAR-4 MknYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MonxTWM2OD15LkG1N|Uh|ryP MlnlV2FPT0WU
KP-N-YS MnzCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVvJR|UxRTdwMUixOEDPxE1? Mm\RV2FPT0WU
NOS-1 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MY\JR|UxRTdwMkW4Nlgh|ryP M174U3NCVkeHUh?=
DMS-114 Mnv1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYr4colRUUN3ME23MlI6PjNzIN88US=> Mn3RV2FPT0WU
NCI-H2030 M1q0W2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1\i[WlEPTB;Nz6zN|Y6QCEQvF2= NX3IR|hFW0GQR1XS
HEL M361R2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NW[2WnZsUUN3ME23MlM4PTJzIN88US=> MlXuV2FPT0WU
CAL-62 MlG1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4PoemlEPTB;Nz6zPVg3PiEQvF2= NELjRVJUSU6JRWK=
COLO-668 NH\QNpJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF\ZN2JKSzVyPUeuOFM6PjdizszN MkXDV2FPT0WU
SKG-IIIa NGLnSZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVf4O4loUUN3ME23MlQ2OzhzIN88US=> M1LtfXNCVkeHUh?=
HCC1419 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2TZVmlEPTB;Nz60Olg{OyEQvF2= MorDV2FPT0WU
OS-RC-2 M3T0U2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoP2TWM2OD15LkS5PFk1KM7:TR?= MYnTRW5ITVJ?
COLO-829 M3e3OGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWfMd|c6UUN3ME23MlY3PDZ|IN88US=> Ml74V2FPT0WU
NB13 NH7ieYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIPUW|VKSzVyPUeuPFI3PjdizszN NUTpZoRFW0GQR1XS
DK-MG MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWLJR|UxRTdwOEOyNFEh|ryP MWTTRW5ITVJ?
SHP-77 M2jrfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWHJR|UxRTdwOE[2OVMh|ryP MWTTRW5ITVJ?
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NCI-H510A NH73eYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1\vbmlEPTB;M{iuOlQ2OSEQvF2= MkjGV2FPT0WU
NMC-G1 NHPFV2hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1q5UGlEPTB;M{iuO|IzOiEQvF2= NF;Sc5dUSU6JRWK=
SW948 M{f0Umdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml7nTWM2OD1|OD64PVQ6KM7:TR?= MmLyV2FPT0WU
MFM-223 MkW2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXjJR|UxRTN6Lkm0N|Eh|ryP MlPnV2FPT0WU
PAN1 M4X6Zmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXG5eHFPUUN3ME2zPE46QTF7IN88US=> NUnSO4s6W0GQR1XS
MZ7-mel M3i5OGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF2wUIpKSzVyPUO5MlEyQTlizszN NFi1VFFUSU6JRWK=
COLO-679 NVGzTHZNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGXYd|VKSzVyPUO5Mlk4ODhizszN MVjTRW5ITVJ?
SW872 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYHINZl2UUN3ME20NE4zODNzIN88US=> MUfTRW5ITVJ?
HT-1197 NGrZXpNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVvJR|UxRTRyLkK4Olkh|ryP MXHTRW5ITVJ?
ES6 Mn74S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV[0eot[UUN3ME20NE41PTV{IN88US=> NH\Jb|RUSU6JRWK=
SNU-387 M3TUNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWm5cm1wUUN3ME20NE45OjN6IN88US=> MVvTRW5ITVJ?
T47D NY\ibllMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MomzTWM2OD12MT6wNVkzKM7:TR?= MUfTRW5ITVJ?
G-402 M2\SW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmnMTWM2OD12MT6wPFA6KM7:TR?= MkDJV2FPT0WU
MZ1-PC NVTOT2pxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV20Xml7UUN3ME20NU45OSEQvF2= NWS0XnJYW0GQR1XS
SW1417 NIPPdYdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn\FTWM2OD12Mj6zNFMh|ryP Mlu3V2FPT0WU
RERF-LC-MS NF3VN25Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFu4Z2RKSzVyPUSyMlUxQDdizszN MnTuV2FPT0WU
SF539 MoTXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFnpeldKSzVyPUSyMlU2OjlizszN M4DJO3NCVkeHUh?=
ESS-1 MnTxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVjN[lFFUUN3ME20N{42PTV6IN88US=> M4TkVXNCVkeHUh?=
RO82-W-1 NWPUfmdTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3rZWGlEPTB;NEOuOlE2OyEQvF2= NWD6ZlJtW0GQR1XS
S-117 Ml\jS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnfhTWM2OD12Mz62PVU5KM7:TR?= NXvucJNlW0GQR1XS
SW756 NUHCVY5tT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXPJWopqUUN3ME20N{45OjR{IN88US=> MXzTRW5ITVJ?
CP66-MEL NVjadZM6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4LiPGlEPTB;NEWuO|E1OyEQvF2= NX\JRVJGW0GQR1XS
H-EMC-SS NFvldW1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlnkTWM2OD12NT63Olk{KM7:TR?= NFjpTIpUSU6JRWK=
NCI-H2052 MojHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYPJR|UxRTR3LkmwNlch|ryP NUDlTlg6W0GQR1XS
SW684 Ml:zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEDNXoxKSzVyPUS3MlAxQDFizszN MYHTRW5ITVJ?
HCC1569 M{XmSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVnJR|UxRTR5LkG2OVgh|ryP M3fvN3NCVkeHUh?=
KGN MlmzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NID0bopKSzVyPUS3MlM{OTFizszN MXHTRW5ITVJ?
LCLC-103H NGj5TYlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYfvTFhSUUN3ME20O{41PDB2IN88US=> NGLwW|NUSU6JRWK=
SW780 NF;hUHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVi4O|hUUUN3ME20O{43OzVzIN88US=> M13HUXNCVkeHUh?=
NCI-H1703 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYHNPZpzUUN3ME20PE4{OzV{IN88US=> MoHyV2FPT0WU
HCC1395 MkjWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFPMTHJKSzVyPUS4Mlc1OzJizszN NX2zSnM6W0GQR1XS

... Click to View More Cell Line Experimental Data

In vivo Gefitinib (100 mg/kg) improves the anti-tumor effect of radiotherapy in LoVo tumor xenografts. [3] Gefitinib treatment of nude mice bearing established human GEO colon cancer xenografts reveals a reversible dose-dependent inhibition of tumor growth because GEO tumors resumes the growth rate of controls at the end of the treatment. [4]

Protocol

Cell Research:[1]
+ Expand
  • Cell lines: NR6, NR6M and NR6W cells
  • Concentrations: 0-2 μM
  • Incubation Time: 72 hours
  • Method: Exponentially growing cells including NR6, NR6M, NR6M and NR6W cells are seeded in sextuple in 96-well plates at a concentration of 2000 cells/well, allowed to adhere and subsequently washed in PBS and incubated overnight in medium containing 0.5% FCS. Cells are then treated with varying concentrations (0-2 μM) of Gefitinib or the solute control DMSO and EGF. The optimal EGF concentration for inducing proliferation of NR6wtEGFR and NR6W cells has previously been determined and hence NR6wtEGFR and NR6W cells are supplemented with 10 nM and 0.1 nM EGF, respectively. EGF is not added to NR6 and NR6M cells. After 72 hours the amount of cells are measured by performing a MTT proliferation assay.
    (Only for Reference)
Animal Research:[3]
+ Expand
  • Animal Models: Female nude mice (cba nu/nu) aged 8–10 weeks are intra-dermal injected with LoVo cells.
  • Formulation: 0.5% polysorbate
  • Dosages: 100 mg/kg
  • Administration: Once daily by oral administration (0.1 mL/10 g body weight) for 14 days
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 89 mg/mL (199.14 mM)
Ethanol 4 mg/mL (8.95 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order:
1% CMC Na
For best results, use promptly after mixing.
30mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 446.90
Formula

C22H24ClFN4O3

CAS No. 184475-35-2
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
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    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03050164 Completed Bioequivalence Study Hunan Kelun Pharmaceutical Co., Ltd. September 8, 2016 Phase 1
NCT02856893 Not yet recruiting NSCLC European Organisation for Research and Treatment of Cancer - EORTC|AstraZeneca March 2017 Phase 2
NCT02976116 Recruiting NSCLC Hutchison Medipharma Limited December 2016 Phase 2
NCT02951637 Not yet recruiting Lung Adenocarcinoma Shanghai Chest Hospital|Ruijin Hospital|Changhai Hospital December 2016 Phase 2
NCT02930954 Not yet recruiting Non-small-cell Lung Cancer Caicun Zhou|Tongji University November 2016 Phase 2
NCT02929693 Enrolling by invitation Cancer Shanghai University of Traditional Chinese Medicine|Shanghai Chest Hospital|Shanghai Pulmonary Hospital, Shanghai, China October 2016 Phase 3

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    I try to dissolve Gefitinib (ZD1839) in 0.5% methylcellulose+0.2% Tween 80 for in vivo experiments. However, when I prepare the mixture, it forms precipitates. Exists another way to dissolve completely the compound?

  • Answer:

    S1025 can be dissovled in both 5% DMSO+corn oil and 5% DMSO+30% PEG 300+5% Tween 80+ddH2O at 2.5 mg/ml clearly. It dissolves in DMSO not readily, please sonicate and warm it in water bath at about 45 degree, and dissolve it clearly first. Then add other solvent.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID