Gefitinib (ZD1839)

Catalog No.S1025

Gefitinib (ZD1839) Chemical Structure

Molecular Weight(MW): 446.90

Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.

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Cited by 91 Publications

12 Customer Reviews

  • Perturbation of EGFR by its ligand EGF and gefitinib (ZD-1839 Iressat; inhibits EGFR) produces opposite responses in the predicted EGFR target genes SOCS2 and NR2E1.

    Mol Syst Biol 2011 7, 486. Gefitinib (ZD1839) purchased from Selleck.

    Effects of shRNA-mediated RECK depletion on EGFR signaling in MEFs. (a) Immunoblotting (IB) of the indicated proteins in wild-type MEFs transduced with the indicated shRNAs and cultured for 48 h in the presence of 0.1% dimethyl sulfoxide (DMSO; vehicle) or 1 mM gefitinib (S1025, Selleck Chemicals,Houston, TX, USA). (b) Immunoprecipitation from whole lysates of cells from panel a with an anti-phosphorylated tyrosine (pTyr) antibody. Precipitates from 500 mg protein in whole cell lysates (upper) or 20 mg protein in whole cell lysates (lower) were analyzed by IB with anti-EGFR antibody.

    Oncogene 2010 30, 737-750. Gefitinib (ZD1839) purchased from Selleck.

  • (B–C) LNCaP (B) and LNCaP-AI (C) cells were transiently transfected with sPLA2-IIa(-800)-Luc (0.5 μg). The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 lM) and Bortezomib (20 μM) without or with EGF (100 ng/ml) for 24 h. Luciferase assay was performed according to a standard protocol with Renilla luciferase as an internal control. Data are presented as the mean (±SD) of duplicate values of a representative experiment that was independently repeated for five times.

    Carcinogenesis 2010 31, 1948–1955 . Gefitinib (ZD1839) purchased from Selleck.

    LNCaP-AI cells were starved in 1% stripped medium for 24 h. The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 μM) and Bortezomib (20 μM) for 24 h. Cell culture medium was collected from each sample and subjected to ELISA for sPLA2-IIa. The condition medium samples were diluted 10 times for ELISA. Average of duplicate samples was converted to nanogram per milliliter against standard curve. The data represent one of five repeated experiments.

     

     

    Carcinogenesis 2010 31, 1948–1955. Gefitinib (ZD1839) purchased from Selleck.

  • Susceptibility of lung cancer cells to cytolytic activity of NK-92 cells after treatment with EGFR inhibitors. Three lung cancer cells were untreated (open circle) or treated with 10 μM erlotinib or gefitinib (black filled square or black filled triangle) for 24 hours. The lung cancer cells were cocultured with NK-92 cells at indicated effector to target ratio (E:T ratio). To determine the specificity of NKG2D-mediated cytolysis of lung cancer cells, NK-92 cells were preincubated with blocking mAb against NKG2D before the assay (gray filled square and triangle) and correspond-ing isotype mAb (open square and triangle).

    J Immunother 2011 34(4), 372-81. Gefitinib (ZD1839) purchased from Selleck.

    Suppression of EGFR signaling by Gefitinib is dose dependent and non- toxic at millimolar concentrations.Results from plaque reduction tests of Hep2 cells infected with VACV(12.5pfu/well) treated with a serial dilution of Gefitinib (1000-0.01 m) and analyzed for (A)proliferation as indicator of cytotoxicity,(B) IC50 of plaque size inhibition, (C)dose dependent inhibition of EGFR-ERK1/2signaling and VACV proteins by Western blot analysis (pEGFR detection was obtained from a different experiment with identicals ettings) and (D)dose dependent inhibition of orthopoxvirus genome replication by real-time PCR.Untreated VACV infectedcells and uninfected cells are shown as controls in the right panel.

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

  • A circular zone of EGFR activated but uninfected cells facilitate virus induced plaque development , efficiently blocked by Gefitinib. (A)Immun of luorescent staining of aplaque reduction tests with Hep2 cells 24,48,72 and 96 h after VACV infection (6.25pfu/chamber). VACV infected cells(green) were visualized by human IgG directed to orthopoxviruses (VIG) detected with a secondary FITC labelled antibody. Uninfected cells with activated EGFR signaling(red) are detected with a secondary Cy3labelled antibody. Positive cells for both , VACV and phosphorylated EGFR are shown in yellow(merge). Cellular nuclei are counterstained with DAPI. (B)Immunofluorescent staining of a plaque reduction test as described for (A) and treated with three different Gefitinib concentrations (1000 nM, 1 nM, and 0.01 nM) . The test was analyzed 96h afterVACV infection.Uninfected cells are shown as controls. Magnification100×.(For interpretation of the references to color in this figure legend , the reader is referred to the web version of the article.)

     

     

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

    The EGF-dependent increased association of PCM1 and AZI1 requires EGFR kinase activity. ZD1839 (Iressa)—EGFR tyrosine kinase inhibitor.

    Mol Biosyst 2011 7, 3223-33. Gefitinib (ZD1839) purchased from Selleck.

  • Reduction of GPER expression in HCC1806 cells after treatment with gefitinib. (A) Concentration dependence. HCC1806 cells were treated with 50-500 nM gefitinib for 48 h. GPER was detected by western blotting. (B) Time dependence. HCC1806 cells were treated with 200 nM gefitinib for 24-96 h. ap<0.05 vs. control; bp<0.001 vs. control. Mean values and SE of three independent experiments are shown. (C) Representative western blotting of concentration dependence. (D) Representative western blotting of time dependence

    Oncol Rep, 2017, 37(2):1212-1218. Gefitinib (ZD1839) purchased from Selleck.

    Inhibition of signaling pathway activation in lung tumor cell lines by kinase inhibitors. Lung tumor cells were cultured in 10% FBS until reaching ∼80% confluence and then the cells were starved in serum-free medium for overnight, followed by 4-hour treatment with the inhibitors. Cell lysates were then prepared and used for determination of the pathway activation signals by the CEER assay.

    Int J Proteomics 2011 215496. Gefitinib (ZD1839) purchased from Selleck.

  • Breast cancer cells were pretreated with 100ng/ml EGF for 15 min and then treated with the indicated concentrations of  Gefitinib for 24 hours.

     

     

    2010 Dr. Zhang of Tianjin Medical University. Gefitinib (ZD1839) purchased from Selleck.

    MTT assay result. 0-500nM Gefitinib treated with H1299, H358, H25 and HCC827 cell lines by 72h, Proliferation rate was tested.

     

     

    2010 Dr. Vicky Tin from University of Hong Kong. Gefitinib (ZD1839) purchased from Selleck.

Purity & Quality Control

Choose Selective EGFR Inhibitors

Biological Activity

Description Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.
Features A potent EGFR tyrosine kinase inhibitor.
Targets
Tyr1173 (NR6W cells) [1] Tyr1173 (NR6wtEGFR cells) [1] Tyr992 (NR6wtEGFR cells) [1] Tyr992 (NR6W cells) [1]
26 nM 37 nM 37 nM 57 nM
In vitro

Gefitinib effectively inhibits all tyrosine phosphorylation sites on EGFR in both the high and low-EGFR-expressing cell lines including NR6, NR6M and NR6W cell lines. The phosphorylation sites Tyr1173 and Tyr992 are less sensitive requiring higher concentrations of Gefitinib for inhibition. Gefitinib effectively blocks the phosphorylation of PLC-γ, with IC50 of 27nM, in NR6W cells. The NR6wtEGFR and NR6M cell lines has low levels of PLC-γ phosphorylation but the level in the NR6M cell line is more resistant to inhibition by Gefitinib with IC50 of 43 nM and 369 nM, respectively. Gefitinib inhibits Akt phosphorylations, with IC50 of 220 and 263nM, in the low-EGFR- and -EGFRvIII-expressing cell lines, respectively. Gefitinib in the dose range from 0.1 to 0.5μM significantly facilitates, rather than abrogates, colony formation of NR6M cells. However, at a concentration of 2 μM Gefitinib completely blocks NR6M colony formation. Gefitinib rapidly and in a dose-dependent manner inhibits EGFR and ERK phosphorylation up to 72 hours after EGF stimulation in both the high- and low-EGFR-expressing cell lines. [1] Gefitinib is the monolayer growth of these EGF-driven untransformed MCF10A cells with an IC50 of 20 nM. [2] The combination of Gefitinib (0.2 μM and 0.5 μM) with irradiation lead to a significant growth inhibition in LoVo cells, compared with radiation alone.. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KB MmfTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1XsZ2lEPTB;MD6wOVQh|ryP M2PJR|E3PTF4NEez
NIH3T3 MXfGeY5kfGmxbjDBd5NigQ>? NFHDXmwxNjJizszN M2TB[lIhcA>? Mle3SG1UVw>? NHzTR5JKdmirYnn0bY9vKG:oIFXHSkB{fGmvdXzheIVlKGi3bXHuJIVz[kJzIHH1eI9xcG:|cHjvdplt[XSrb36ge4l1cCCLQ{WwJI9nKDBwMEG0OEDPxE1? M3rNNFE3PDhyMki0
COS7 NIPhOVhHfW6ldHnvckBCe3OjeR?= MlrDPVYh|ryP NIjiZXJKdmirYnn0bY9vKG:oIHj1cYFvKEqDS{KgWlYyP0ZibYX0ZY51KGW6cILld5Nm\A>? NYrhR4RUOTdzN{i3NlI>
A549 NF3td21EgXSxdH;4bYMhSXO|YYm= M1zpO|k3KGh? MmiySG1UVw>? NGGweZpKSzVyPUGzMlU6KM7:TR?= MYKxPFMyOzhyNx?=
K562 MorJR5l1d3SxeHnjJGF{e2G7 M4DsW|k3KGh? NHX4TnhFVVOR MVzJR|UxRTlwM{[g{txO MormNVg{OTN6MEe=
MDA-MB-231 MmL0R5l1d3SxeHnjJGF{e2G7 NHTzO2s6PiCq NULJU45ETE2VTx?= MojXTWM2OD1|Nj62NUDPxE1? NGTCdlUyQDNzM{iwOy=>
MOLT4 NGr6SVZEgXSxdH;4bYMhSXO|YYm= M2TOb|k3KGh? M124WGROW09? NVPORnZ6UUN3ME2xOU4xOiEQvF2= MlW2NVg{OTN6MEe=
MCF7 NWrWSoVpS3m2b4TvfIlkKEG|c3H5 NGrLV2I2OCEQvF2= M2e3S|czKGh? M1jTWGlEPTB;MUKuNFUh|ryP M{W3NlE5PzdzOEG5
BT474 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHHWPGY4OiCq NHPWO5ZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFLUOFc1KGOnbHzzJI93\XKneIDy[ZN{cW6pIFXSRoIzKHerdHigSWM2OCCxZjCwMlMh|ryP MkLuNVkxOjh2MkS=
HN5 MlO3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUTwe5pxPzJiaB?= MXHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiQNTDj[YxteyCxdnXy[ZhxemW|c3nu[{BGT0[UIIfpeIghTUN3MDDv[kAxNjBzIN88US=> MXixPVAzQDR{NB?=
BA/F3 M{XMVGN6fG:2b4jpZ{BCe3OjeR?= M1j0UFczKGh? NUfCW4piS3m2b4TvfIlkcXS7IHHnZYlve3RibX;1d4UhSkFxRkOgZ4VtdHNiZYjwdoV{e2mwZzDFS2ZTKEx6NUjSJI12fGGwdDD3bZRpKEmFNUCgc4YhOC5yMUC4JO69VQ>? NFn2boYyQTJ|OUKyPS=>
NIH3T3 M1Lnfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGX3XW84OiCq NGXucXZIem:5dHigbY5pcWKrdHnvckBw\iCvb4Xz[UBPUUhxM2SzJINmdGy|IHX4dJJme3Orbneg[ZJDOiCpZX7lJJdqfGhiSVO1NEBw\iByLkW0JO69VQ>? NX7ncodqOTl4NkWzO|c>
MCF7 MX MkfpSpVv[3Srb36gRZN{[Xl? Ml3pN|AhdWmw NH;iVIdKdmirYnn0bY9vKG:oIFLDVnAh\XiycnXzd4VlKHerdHigTWM2OCCxZjCxMlEh|ryP Mn2yNVk6OzJ7NkC=
A431 NYrjblQ6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYixNEDPxE1? NHHTSFY4OiCq MkLOS2k2OD1yLkWxOEDPxE1? M4\ER|E6QTZ7NE[1
A431 NXvJe4hmU2mwYYPlJGF{e2G7 MWCwMlEh|ryP MYSxJIg> M4fW[mlvcGmkaYTpc44hd2ZiRVfGMYlv\HWlZXSgSWdHWiCyaH;zdIhwenmuYYTpc44> M2X3e|E6QTZ7NE[1
A431 NFnPPGpMcW6jc3WgRZN{[Xl? NUCwdHJxOTByIN88US=> M4HTPFExKG2rbh?= MVnQdo9td26pZXSgbY5pcWKrdH;yfUBi[3Srdnn0fUBw\iCHR1[tbY5lfWOnZDDFS2ZTKHCqb4PwbI9zgWyjdHnvci=> MVOxPVk3QTR4NR?=
Sf9 MXvLbY5ie2ViQYPzZZk> MWixNEBucW5? NHnZZ3VFVVOR NUnadnpUUW6qaXLpeIlwdiCxZjDFS2ZTKGW6cILld5Nm\CCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKHKnY3XweI9zKGG3dH;wbI9{eGixconsZZRqd25id3n0bEBKSzVyIH;mJFAvODN|IN88US=> NWr4S|ZIOjByNU[0NlU>
NCI-H1975 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3zVUFUh|ryP NX7YSZVHPzJiaB?= MYfJR|UxRThwMk[g{txO MoLTNlAyPTF4N{C=
NCI-H1975 NYC3dol1U2mwYYPlJGF{e2G7 NHnoeWsyOCEQvF2= M4mybGlvcGmkaYTpc44hd2ZiRVfGVkBifXSxcHjvd5Bpd3K7bHH0bY9v M1TGfVIxOTVzNkew
NCI-H1975 M4rhV2Z2dmO2aX;uJGF{e2G7 MWCxNEDPxE1? NEjpb2tKdmirYnn0bY9vKG:oIFXSRmIzKGG3dH;wbI9{eGixconsZZRqd25? NUP5T4lOOjBzNUG2O|A>
NCI-H1975 NE\tOWFHfW6ldHnvckBCe3OjeR?= MYG1JO69VQ>? NWTCNXhXPDhiaB?= NXn1N2RYUW6mdXP0bY9vKG:oIHHwc5B1d3OrczDhd5Nme3OnZDDhd{Bi[3SrdnH0bY9vKG:oIHPhd5Bie2VvMx?= NG\wUmwzODF3MU[3NC=>
A375 NX;2c|RRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4DsSFQ5KGh? MWfJR|UxRTJyLkW1JO69VQ>? NUT6bYdyOjB|MES1N|c>
SMMC7721 MonIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlqyOFghcA>? NH7KZmJKSzVyPUOzMlEzKM7:TR?= NWfyS3dDOjB|MES1N|c>
DU145 M2XxNWN6fG:2b4jpZ{BCe3OjeR?= NIn0dpM2KGR? NYHMd5o6TE2VTx?= NXLCOIZzUUN3ME20Nk4yPyEQvF2= NEPCXpEzODR4NkW1OS=>
MIAPaCa2 MmHOR5l1d3SxeHnjJGF{e2G7 MWC1JIQ> NUHnTnMyTE2VTx?= M2HYOWlEPTB;NUCuOlIh|ryP NV;sV2dPOjB2Nk[1OVU>
PANC1 MlPGR5l1d3SxeHnjJGF{e2G7 NX\0V|I5PSCm NEfjb3lFVVOR MlTLTWM2OD12MD60NkDPxE1? MY[yNFQ3PjV3NR?=
PC9 NF;ZVmdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIC5TmxCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGVITlJvZHXmbYNq\W62IHj1cYFvKFCFOTDj[YxteyC5aYToJGVEPTBib3[gNE4xOTdizszN MXyyNVIxQDhyMh?=
PC9 NV7qUGk5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoDVRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDndo94fGhvcnXzbZN1[W62IHj1cYFvKFCFOTDj[YxteyCneIDy[ZN{cW6pIFXHSnIhTTd2Nm;BO|UxN1R5OUDNJI12fGGwdDD3bZRpKEWFNUCgc4YhPyEQvF2= NEK0b|UzOTJyOEiwNi=>
PC3 MlfuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYrW[5F2OTBizszN MXm3NkBp NHzLcJBFVVOR Mkm3TWM2OD15LkSg{txO M1TqVVIyOzV|NUS2
MDA-MB-468 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXPCU|lFOTByIN88US=> NFPLWng1QCCq MnTUSG1UVw>? NV\kOVhDSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJk> NITi[nczOTR{OU[zNi=>
Plasmodium falciparum M4PmUpFJXFNicILv[oltcW6pIH\vdkBqdmirYnn0c5J{KG:oIGDsZZNud2SrdX2g[oFt[2myYYL1cUBxem:uaX\ldoF1cW:w MYeyNVgyPzB2NR?=
NCI-H1993 NXW4TYZWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUi3NkBp NY\sR5ZJTE2VTx?= NWrBW2NDUUN3ME2wMlEh|ryP NGXsXXAzOTlzNkSzNy=>
NCI-H292 NILUZWdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoToO|IhcA>? Mk\OSG1UVw>? M3jrTGlEPTB;MD6wNkDPxE1? NYDpZnZJOjF7MU[0N|M>
A431 NYm2WHhrU2mwYYPlJGF{e2G7 MV2yJIg> MkC1SG1UVw>? NUKyPINDUW6qaXLpeIlwdiCxZjDFS2ZTKHCqb4PwbI9zgWyjdHnvckBqdiCHR1[td5RqdXWuYYTl[EBpfW2jbjDBOFMyKHerdHigTWM2OCCxZjCwMlAxOSEQvF2= M2\HRVIzOTBzMUOy
MCF7 MnX4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1;wV|ExOMLizsznM41N MYO0PEBp NITuXlFFVVOR NWjiXZZHSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJI93\XKneIDy[ZN{cW6pIFXHSnIh\2WwZTD3bZRpKEmFNUCgc4YhOTJwMEWg{txO MWSyNlEyQTF|MB?=
BT474 NGLpNmhEgXSxdH;4bYMhSXO|YYm= NWXXR2pxOTBizszN MWS0PEBp NYSzcY1xS3m2b4TvfIlkcXS7IHHnZYlve3Ric3XyeY0ue3Sjco\l[EBpfW2jbjDCWFQ4PCClZXzsdy=> NXHTSYJ[OjJzM{izNFg>
MCF7 NULyVI1qS3m2b4TvfIlkKEG|c3H5 MmS0NVAh|ryP NWLWNJpmPDhiaB?= NFnxNWhEgXSxdH;4bYNqfHliYXfhbY5{fCC|ZYL1cU1{fGG{dnXkJIh2dWGwIF3DSlch[2WubIOgc5Zmei2neIDy[ZN{cW6pIFXyZmIzKGOnbHzz NGr5SJkzOjF|OEOwPC=>
HCC827 M{DQR2tqdmG|ZTDBd5NigQ>? NVzYVmgyOC5zMkWg{txO MXiyOEBp MW\EUXNQ NYrtToNmUW6qaXLpeIlwdiCxZjDFS2ZTKGSnbDDFO|Q3NUF5NUCgcZV1[W62IGmxNFY5KGG3dH;wbI9{eGixconsZZRqd25? NFn0cXkzOjN|OUO0Ni=>
HCC827 Mo\JT4lv[XOnIFHzd4F6 M2LHflAvOTJ3IN88US=> NFLkW5EzPCCq MV\EUXNQ NFSwVnZKdmirYnn0bY9vKG:oIFXHSnIh\GWuIFW3OFYuSTd3MDDteZRidnRvbXXkbYF1\WRiRYLrJJBpd3OyaH;yfYxifGmxbh?= MoPLNlI{Ozl|NEK=
HCC827 M4nwfmtqdmG|ZTDBd5NigQ>? MWWwMlEzPSEQvF2= M{HEWFI1KGh? NXzsNXpsTE2VTx?= Ml7tTY5pcWKrdHnvckBw\iCHR1\SJIRmdCCHN{S2MWE4PTBibYX0ZY51NW2nZHnheIVlKEGtdDDwbI9{eGixconsZZRqd25? MlW4NlI{Ozl|NEK=
A431 NWi2NJdCU2mwYYPlJGF{e2G7 MlHYNVAxKM7:TR?= M{HVbVMxKG2rbh?= MlK3TY5pcWKrdHnvckBw\iCHR1\SJIRmemm4ZXSg[pJwdSCqdX3hckBCPDNzIHPlcIwhdWWvYoLhcoUh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBxd2y7LVfseU1VgXJicHjvd5Bpd3K7bHH0bY9vKHerdHigTWM2OCCxZjCxMlA5KM7:TR?= MYGyNlY6QDd6Mh?=
H460 MmD3R5l1d3SxeHnjJGF{e2G7 NXLldIQ2PzJiaB?= MVjJR|UxRTVwNUmg{txO NEfZflMzOjd{N{S0PS=>
HT-29 Mk\LR5l1d3SxeHnjJGF{e2G7 M1vlT|czKGh? M1zUfGlEPTB;Mz6zOkDPxE1? MlLpNlI4Ojd2NEm=
SGC7901 M2CxS2N6fG:2b4jpZ{BCe3OjeR?= NFfQNGI4OiCq MVPJR|UxRTFyLkK2JO69VQ>? MVuyNlczPzR2OR?=
A498 NGPZXYpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEF2OUigZ4VtdHNiZYjwdoV{e2mwZzDFS2ZTKGGwZDDTVmMhf2m2aDDJR|UxKG:oIEiuOkDPxE1? NH25RWMzOjhzOEi0PC=>
Calu3 NUL1bmUyS3m2b4TvfIlkKEG|c3H5 MonsNVAh|rypL33M Mm\HO|IhcA>? M{\FRWlEPTB;MT62JO69VQ>? NXXNToM4OjNzMU[xOlg>
FADU MX3DfZRwfG:6aXOgRZN{[Xl? NGLDSZAyOCEQvHevcWw> NYj2Z2tKPzJiaB?= NVrCTlg5UUN3ME2wMlA2KM7:TR?= MX6yN|EyPjF4OB?=
HCC827 MoDMR5l1d3SxeHnjJGF{e2G7 M3njOVExKM7:Zz;tUC=> MV:3NkBp MYrJR|UxRTBwMECxOUDPxE1? Mn[1NlMyOTZzNki=
PC9 M2LZc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml\pNVAh|rypL33M MV:3NkBp M2jTVmlEPTB;MD6wNFE1KM7:TR?= NGLZeI8zOzFzNkG2PC=>
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NCI-H2170 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVPN[WM2UUN3ME2yMlM1Pzh|IN88US=> MnnYV2FPT0WU
EW-18 M1HQVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{HyXWlEPTB;Mj6zOVQ4OSEQvF2= MmXSV2FPT0WU
KU812 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3vIeGlEPTB;Mj6zOlc4QSEQvF2= M2DC[3NCVkeHUh?=
NY NYXn[3RYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIHFVXJKSzVyPUKuN|czPyEQvF2= MXPTRW5ITVJ?
HTC-C3 NHTie3NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWHtRZRHUUN3ME2yMlQzOjZ5IN88US=> M3z5eXNCVkeHUh?=
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HH NEG1clBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnPRTWM2OD1{LkS5PFU1KM7:TR?= Mn;5V2FPT0WU
MKN7 NF3BVVhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkDHTWM2OD1{Lk[yO|Q5KM7:TR?= NVLuWlhDW0GQR1XS
TE-8 Mn3tS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX7MV3FJUUN3ME2yMlY{PjV|IN88US=> M4rHTXNCVkeHUh?=
MC-IXC NIXlTGZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYXnOIF5UUN3ME2yMlY1PDV|IN88US=> M1H2ZXNCVkeHUh?=
DMS-79 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlLZTWM2OD1{Lk[5Nlg1KM7:TR?= NHPRZXlUSU6JRWK=
Detroit562 M3\MdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX\JbpZNUUN3ME2yMlY6QDR3IN88US=> MXfTRW5ITVJ?
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PC-3 NFy1T2NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXP0UHQ1UUN3ME2yMlg{OzF5IN88US=> MXHTRW5ITVJ?
OVCAR-3 MnjIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXTvWWlJUUN3ME2yMlg2PTl2IN88US=> NVrFSot[W0GQR1XS
KYSE-510 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWfJR|UxRTJwOE[3PFYh|ryP NVHKfYNFW0GQR1XS
HC-1 NXGze3lvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnXOTWM2OD1{Lkm0NFEzKM7:TR?= MmnaV2FPT0WU
NCI-H1693 M{DGV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoP4TWM2OD1{Lkm1PFk4KM7:TR?= NHPYTFdUSU6JRWK=
DU-4475 NHe0[ZJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXLRXlNyUUN3ME2yMlk6ODV{IN88US=> MX7TRW5ITVJ?
COLO-680N Mn7ES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml25TWM2OD1|LkCwOFk2KM7:TR?= MmfDV2FPT0WU
MN-60 NYHMbIR6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIi2PJNKSzVyPUOuNFQ6PzRizszN MVPTRW5ITVJ?
BCPAP NIS1ZVZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFS1eVhKSzVyPUOuNFgzQTVizszN NVLHeIlsW0GQR1XS
647-V NHz5V3RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVj1d4lrUUN3ME2zMlE3OjJizszN MnzkV2FPT0WU
HT-1376 MnTsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mmq3TWM2OD1|LkKwOVA3KM7:TR?= MlrEV2FPT0WU
NCI-H1793 Mn3zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIezTYdKSzVyPUOuNlExQThizszN MkfsV2FPT0WU
SW1463 NV[2N|VQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYnJR|UxRTNwMk[xPVIh|ryP NX3aSpB4W0GQR1XS
NCI-H727 NHvGOVFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{nvV2lEPTB;Mz6zPFU5PCEQvF2= M3zRdnNCVkeHUh?=
LB771-HNC M2GzeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXfJR|UxRTNwM{mxNlYh|ryP MnPxV2FPT0WU
D-542MG Mn7rS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml\PTWM2OD1|LkS0NlIzKM7:TR?= NUjJVWtOW0GQR1XS
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C8166 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnLtTWM2OD1|LkS2OVIyKM7:TR?= NELEOVVUSU6JRWK=
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TE-1 MknzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1ywNGlEPTB;Mz61PVE3QSEQvF2= NFXTWVBUSU6JRWK=
OAW-28 MlrmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVnDbGk5UUN3ME2zMlU6PDJ4IN88US=> M3qzZ3NCVkeHUh?=
SK-LU-1 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWPJR|UxRTNwNkC2OFUh|ryP MoHvV2FPT0WU
CaR-1 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGrZVpVKSzVyPUOuOlExPDhizszN NYW5O|huW0GQR1XS
A2780 MnviS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYTsOnBXUUN3ME2zMlY1ODh7IN88US=> MlX3V2FPT0WU
C2BBe1 Mlv2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2OzfGlEPTB;Mz62OFQxPyEQvF2= NX\R[mp7W0GQR1XS
SK-MES-1 M3i3bmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mkf3TWM2OD1|Lk[0OlE1KM7:TR?= NVX0XXlRW0GQR1XS
PANC-10-05 MlTjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3\1ZWlEPTB;Mz63NlA4OiEQvF2= M2\CTHNCVkeHUh?=
J-RT3-T3-5 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIr2fmlKSzVyPUOuO|U1ODZizszN MXzTRW5ITVJ?
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NCI-H28 NGXsbWRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmroTWM2OD1|LkiyPVYyKM7:TR?= M4npTHNCVkeHUh?=
NCI-H1155 M2LGfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{DJ[WlEPTB;Mz64OFE2QSEQvF2= M{D1ZnNCVkeHUh?=
KURAMOCHI MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVOxVFNEUUN3ME2zMlg3ODV4IN88US=> NUL0e|VbW0GQR1XS
NCI-H69 M4DPUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHfIT|BKSzVyPUOuPVAyPzhizszN MYXTRW5ITVJ?
IST-MES1 M3nKNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUWzTpp5UUN3ME2zMlk1ODJ7IN88US=> MmDRV2FPT0WU
NH-12 NXi2c5EyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkOyTWM2OD1|Lkm0NVg4KM7:TR?= MlvvV2FPT0WU
JVM-2 Mk\WS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWjJR|UxRTNwOUS3OVIh|ryP MoXJV2FPT0WU
EW-16 MmPCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnLVTWM2OD1|Lkm1OVc4KM7:TR?= NEXhdlRUSU6JRWK=
NCI-H441 MmPHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkXtTWM2OD1|Lkm3NFE1KM7:TR?= M{jBTnNCVkeHUh?=
RCC10RGB MnjtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoT2TWM2OD12LkCxNlQ3KM7:TR?= MXfTRW5ITVJ?
TCCSUP NX\4[JM{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{\OR2lEPTB;ND6xNVA2KM7:TR?= MmXJV2FPT0WU
HL-60 Mmj5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnXXTWM2OD12LkGyNlQyKM7:TR?= MVHTRW5ITVJ?
EW-11 NHXTVFBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mnz6TWM2OD12LkGzNlYyKM7:TR?= NHPE[mhUSU6JRWK=
KARPAS-299 NF\Dc5dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFf3UXlKSzVyPUSuN|ExOTNizszN NGrkVGlUSU6JRWK=
SNU-C2B MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWLQTVlQUUN3ME20MlM1Pjh4IN88US=> MlvUV2FPT0WU
LU-139 MnLiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1\EVmlEPTB;ND6zO|I1PyEQvF2= NHmzO2tUSU6JRWK=
HCC1937 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFewTHlKSzVyPUSuN|c6QTNizszN MVHTRW5ITVJ?
GR-ST NEP4[2dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXzGNFFxUUN3ME20MlQ{OThizszN NH\QcoRUSU6JRWK=
SK-OV-3 M3Xjcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXrJR|UxRTRwNE[wOFIh|ryP NY\PbW1sW0GQR1XS
A172 M1nrOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWHZPVJnUUN3ME20MlU2PTJzIN88US=> MmjhV2FPT0WU
NB10 M{\CW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NI\nUY5KSzVyPUSuOVk4QDFizszN Mm\LV2FPT0WU
P30-OHK M3mxfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3rZb2lEPTB;ND64NVkzQSEQvF2= M1:xT3NCVkeHUh?=
KYSE-520 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXTtSHdyUUN3ME20Mlg5ODF6IN88US=> NWj5XpY5W0GQR1XS
KNS-62 NVnxV45XT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3;n[GlEPTB;ND65NFgxPyEQvF2= MXPTRW5ITVJ?
ES1 M3P0TGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX7tU297UUN3ME20Mlk3ODN3IN88US=> NWP6d4xoW0GQR1XS
KOSC-2 NETTcG1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGXrW41KSzVyPUSuPVk6PTlizszN NFewcFZUSU6JRWK=
TE-9 MoPsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3S4bGlEPTB;NT6wNVUzPSEQvF2= M4HWRXNCVkeHUh?=
NCI-H1838 NIDHNHhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUHJR|UxRTVwMEKwO|Yh|ryP M2rHbXNCVkeHUh?=
KP-N-YN MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYDJR|UxRTVwMUK1PVEh|ryP M2HpN3NCVkeHUh?=
ALL-PO NEnHPGtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVOxS2pDUUN3ME21MlIzPTh5IN88US=> NWeyXldpW0GQR1XS
NOMO-1 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWjkNHNvUUN3ME21MlI{OTd4IN88US=> MWXTRW5ITVJ?
CGTH-W-1 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4fFXWlEPTB;NT6zPFU5PCEQvF2= M2\VdnNCVkeHUh?=
ATN-1 NGPidnpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlvDTWM2OD13LkSxNVg1KM7:TR?= NF:zNGdUSU6JRWK=
AGS M1nMdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX7JR|UxRTVwNEm2NVgh|ryP NFzIdJFUSU6JRWK=
SW13 NYfBfHBnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYjJR|UxRTVwNUOyOlUh|ryP Mn71V2FPT0WU
SW1990 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3niR2lEPTB;NT62NlI5PSEQvF2= M3nHdnNCVkeHUh?=
BFTC-905 NF3tTnJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHjBVlJKSzVyPUWuOlU5PzhizszN NGDLbWlUSU6JRWK=
RMG-I MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXXJR|UxRTVwNkW5OFIh|ryP MmjsV2FPT0WU
HD-MY-Z MnTXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGK4NmZKSzVyPUWuOlg2QDFizszN Mnj4V2FPT0WU
HCC1187 NUTZ[Ic{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYLHb21qUUN3ME21MlcyODN{IN88US=> NWrse5JqW0GQR1XS
HCC2998 NVP4flFST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1fSNmlEPTB;NT63NVg4PyEQvF2= M{XjfXNCVkeHUh?=
RT-112 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXPqRY5nUUN3ME21MlkxOjR3IN88US=> NV3pOVhjW0GQR1XS
CAKI-1 NEXCbGVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MonMTWM2OD14LkCwPVg2KM7:TR?= MkHNV2FPT0WU
HDLM-2 MnfCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2LkZWlEPTB;Nj6wNVQ5QSEQvF2= MnjqV2FPT0WU
TE-10 M1yxd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{\NN2lEPTB;Nj6wOFg{OyEQvF2= M{LhbXNCVkeHUh?=
KE-37 Mn7wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmLETWM2OD14LkC3NlU5KM7:TR?= NF\aUI1USU6JRWK=
RCM-1 NV\WUWVZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX\4U4NWUUN3ME22MlA4PzF7IN88US=> NY\pVXpLW0GQR1XS
Mewo MkPkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHXCVnRKSzVyPU[uNVE1QDdizszN NHzlZVdUSU6JRWK=
L-428 NVzPN|JmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWHvVJNQUUN3ME22MlE5ODN5IN88US=> MVTTRW5ITVJ?
8-MG-BA M1;YVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVvwNJlIUUN3ME22MlE6Ozd7IN88US=> M{\Ye3NCVkeHUh?=
SK-MEL-28 NGq4dWNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mlj4TWM2OD14LkKwPVY4KM7:TR?= M2TWfHNCVkeHUh?=
VA-ES-BJ MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFfQfZNKSzVyPU[uN|A1PjJizszN NXr3RVBCW0GQR1XS
CAL-120 NWS1flVqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MULJR|UxRTZwNEWxOlIh|ryP MmL0V2FPT0WU
GAMG NVP4XHFUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUXZU5ZyUUN3ME22MlUxPjJizszN MX3TRW5ITVJ?
MSTO-221H NXroUoNCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MofpTWM2OD14LkWxNVch|ryP M3[1OnNCVkeHUh?=
GCT MnW3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXPJR|UxRTZwNUOzPFch|ryP M4Tac3NCVkeHUh?=
SH-4 NGLyVopIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M17UUGlEPTB;Nj61PFg1PCEQvF2= MYjTRW5ITVJ?
Calu-3 M{C1[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2DzdWlEPTB;Nj62OVU3QCEQvF2= M4TPNXNCVkeHUh?=
SK-HEP-1 NGO5VmhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF7zbWFKSzVyPU[uO|E4PTNizszN MWXTRW5ITVJ?
PFSK-1 NVPMPFFXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVLHXlBIUUN3ME22Mlc{PDd6IN88US=> NYrs[HV4W0GQR1XS
NB12 NIW4bppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVrJR|UxRTZwN{i0NFQh|ryP NWLoUpVbW0GQR1XS
A388 NFX4NoZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFHjelhKSzVyPU[uPFk2OiEQvF2= Mk\RV2FPT0WU
KLE M2f0bGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mor4TWM2OD14Lkm2OVE3KM7:TR?= NXTnRo5mW0GQR1XS
TE-11 NGe0XGlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmrGTWM2OD15LkC0PVY4KM7:TR?= MVjTRW5ITVJ?
NCI-H2405 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUXJR|UxRTdwMEizPVMh|ryP NEXnPHNUSU6JRWK=
OVCAR-4 M4HaWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEHseIRKSzVyPUeuNVU{PSEQvF2= NH7rW5ZUSU6JRWK=
KP-N-YS NVWxcZl7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1vWZmlEPTB;Nz6xPFE1KM7:TR?= M1LodXNCVkeHUh?=
NOS-1 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHO1NG9KSzVyPUeuNlU5OjhizszN Mn6xV2FPT0WU
DMS-114 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEPaW3ZKSzVyPUeuNlk3OzFizszN NGfDe3BUSU6JRWK=
NCI-H2030 NVLZT4ZNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXLJR|UxRTdwM{O2PVgh|ryP MXvTRW5ITVJ?
HEL NV3KZZFnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2TOZ2lEPTB;Nz6zO|UzOSEQvF2= M3r5XXNCVkeHUh?=
CAL-62 M3LrR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGnQXYVKSzVyPUeuN|k5PjZizszN NVjIUFdMW0GQR1XS
COLO-668 MnKxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4TnVGlEPTB;Nz60N|k3PyEQvF2= MYPTRW5ITVJ?
SKG-IIIa MkHhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVnJR|UxRTdwNEWzPFEh|ryP NIroZ|JUSU6JRWK=
HCC1419 NXeyeXZRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEnZempKSzVyPUeuOFY5OzNizszN MYrTRW5ITVJ?
OS-RC-2 NFuxc4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVX2coxlUUN3ME23MlQ6QDl2IN88US=> MlvPV2FPT0WU
COLO-829 NGDscIdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUfJR|UxRTdwNk[0OlMh|ryP MXHTRW5ITVJ?
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NB14 Mn\yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2LXW2lEPTB;M{iuOFU1PCEQvF2= MoTDV2FPT0WU
D-423MG M{nodmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGPCZWVKSzVyPUO4MlQ5PjlizszN M3nmNnNCVkeHUh?=
NCI-H510A MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml\qTWM2OD1|OD62OFUyKM7:TR?= NFXCWJlUSU6JRWK=
NMC-G1 M{LoRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVy2ZXY1UUN3ME2zPE44OjJ{IN88US=> M4XuSXNCVkeHUh?=
SW948 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWW2SYZzUUN3ME2zPE45QTR7IN88US=> MX7TRW5ITVJ?
MFM-223 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn;JTWM2OD1|OD65OFMyKM7:TR?= NIK2RnZUSU6JRWK=
PAN1 NHztfJJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4\LNmlEPTB;M{iuPVkyQSEQvF2= NUnwOWVWW0GQR1XS
MZ7-mel NH\6ZXlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVzJR|UxRTN7LkGxPVkh|ryP M37EdnNCVkeHUh?=
COLO-679 NX3aO4g{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYfJR|UxRTN7Lkm3NFgh|ryP NEnOcGRUSU6JRWK=
SW872 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUDJR|UxRTRyLkKwN|Eh|ryP M4DFeXNCVkeHUh?=
HT-1197 NWfwSZdLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHPLfJNKSzVyPUSwMlI5PjlizszN Mn\ZV2FPT0WU
ES6 M4Llemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYT2TWlrUUN3ME20NE41PTV{IN88US=> M4D6enNCVkeHUh?=
SNU-387 NFvqOFlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkHETWM2OD12MD64NlM5KM7:TR?= MYfTRW5ITVJ?
T47D NYqzXGlST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NInEZ2FKSzVyPUSxMlAyQTJizszN NWS2OZpJW0GQR1XS
G-402 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn3LTWM2OD12MT6wPFA6KM7:TR?= NFTWZo5USU6JRWK=
MZ1-PC NUjhV5BHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUTBSHV3UUN3ME20NU45OSEQvF2= M3L3PHNCVkeHUh?=
SW1417 Ml\QS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWP6VGlUUUN3ME20Nk4{ODNizszN NXTYRYZuW0GQR1XS
RERF-LC-MS M1XsTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFvNfXJKSzVyPUSyMlUxQDdizszN MYPTRW5ITVJ?
SF539 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4XrW2lEPTB;NEKuOVUzQSEQvF2= MoTPV2FPT0WU
ESS-1 M{PMN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M33oVmlEPTB;NEOuOVU2QCEQvF2= NF:yWXdUSU6JRWK=
RO82-W-1 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MonzTWM2OD12Mz62NVU{KM7:TR?= M4\QWnNCVkeHUh?=
S-117 MkD3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4fPfGlEPTB;NEOuOlk2QCEQvF2= NV3JbXFGW0GQR1XS
SW756 MnHIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2fSfGlEPTB;NEOuPFI1OiEQvF2= M{jJTXNCVkeHUh?=
CP66-MEL NXnGTHY{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVvJR|UxRTR3LkexOFMh|ryP M1;LcHNCVkeHUh?=
H-EMC-SS NEfaRm1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV\JR|UxRTR3Lke2PVMh|ryP MnTvV2FPT0WU
NCI-H2052 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV7CTVhxUUN3ME20OU46ODJ5IN88US=> NFrscZJUSU6JRWK=
SW684 MkfGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVmz[IlGUUN3ME20O{4xODhzIN88US=> MUPTRW5ITVJ?
HCC1569 MoT3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MY\JR|UxRTR5LkG2OVgh|ryP NUKzVotWW0GQR1XS
KGN NF\KWlNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4TQOmlEPTB;NEeuN|MyOSEQvF2= NIjHfoxUSU6JRWK=
LCLC-103H MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2HjbGlEPTB;NEeuOFQxPCEQvF2= NX6zb4RtW0GQR1XS
SW780 M1nhSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{\YfmlEPTB;NEeuOlM2OSEQvF2= M4fHcHNCVkeHUh?=
NCI-H1703 M2D2O2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV3JR|UxRTR6LkOzOVIh|ryP MnjDV2FPT0WU
HCC1395 M{n0Wmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUfJR|UxRTR6Lke0N|Ih|ryP NYjxeIl6W0GQR1XS

... Click to View More Cell Line Experimental Data

In vivo Gefitinib (100 mg/kg) improves the anti-tumor effect of radiotherapy in LoVo tumor xenografts. [3] Gefitinib treatment of nude mice bearing established human GEO colon cancer xenografts reveals a reversible dose-dependent inhibition of tumor growth because GEO tumors resumes the growth rate of controls at the end of the treatment. [4]

Protocol

Cell Research:[1]
+ Expand
  • Cell lines: NR6, NR6M and NR6W cells
  • Concentrations: 0-2 μM
  • Incubation Time: 72 hours
  • Method: Exponentially growing cells including NR6, NR6M, NR6M and NR6W cells are seeded in sextuple in 96-well plates at a concentration of 2000 cells/well, allowed to adhere and subsequently washed in PBS and incubated overnight in medium containing 0.5% FCS. Cells are then treated with varying concentrations (0-2 μM) of Gefitinib or the solute control DMSO and EGF. The optimal EGF concentration for inducing proliferation of NR6wtEGFR and NR6W cells has previously been determined and hence NR6wtEGFR and NR6W cells are supplemented with 10 nM and 0.1 nM EGF, respectively. EGF is not added to NR6 and NR6M cells. After 72 hours the amount of cells are measured by performing a MTT proliferation assay.
    (Only for Reference)
Animal Research:[3]
+ Expand
  • Animal Models: Female nude mice (cba nu/nu) aged 8–10 weeks are intra-dermal injected with LoVo cells.
  • Formulation: 0.5% polysorbate
  • Dosages: 100 mg/kg
  • Administration: Once daily by oral administration (0.1 mL/10 g body weight) for 14 days
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 89 mg/mL (199.14 mM)
Ethanol 4 mg/mL (8.95 mM)
Water Insoluble
In vivo Add solvents individually and in order:
1% CMC Na
30mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 446.90
Formula

C22H24ClFN4O3

CAS No. 184475-35-2
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
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    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03050164 Completed Bioequivalence Study Hunan Kelun Pharmaceutical Co., Ltd. September 8, 2016 Phase 1
NCT02856893 Not yet recruiting NSCLC European Organisation for Research and Treatment of Cancer - EORTC|AstraZeneca March 2017 Phase 2
NCT02976116 Recruiting NSCLC Hutchison Medipharma Limited December 2016 Phase 2
NCT02951637 Not yet recruiting Lung Adenocarcinoma Shanghai Chest Hospital|Ruijin Hospital|Changhai Hospital December 2016 Phase 2
NCT02930954 Not yet recruiting Non-small-cell Lung Cancer Caicun Zhou|Tongji University November 2016 Phase 2
NCT02929693 Enrolling by invitation Cancer Shanghai University of Traditional Chinese Medicine|Shanghai Chest Hospital|Shanghai Pulmonary Hospital, Shanghai, China October 2016 Phase 3

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    I try to dissolve Gefitinib (ZD1839) in 0.5% methylcellulose+0.2% Tween 80 for in vivo experiments. However, when I prepare the mixture, it forms precipitates. Exists another way to dissolve completely the compound?

  • Answer:

    S1025 can be dissovled in both 5% DMSO+corn oil and 5% DMSO+30% PEG 300+5% Tween 80+ddH2O at 2.5 mg/ml clearly. It dissolves in DMSO not readily, please sonicate and warm it in water bath at about 45 degree, and dissolve it clearly first. Then add other solvent.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID