Gefitinib (ZD1839)

Catalog No.S1025

Gefitinib (ZD1839) Chemical Structure

Molecular Weight(MW): 446.90

Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.

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Cited by 90 Publications

11 Customer Reviews

  • Perturbation of EGFR by its ligand EGF and gefitinib (ZD-1839 Iressat; inhibits EGFR) produces opposite responses in the predicted EGFR target genes SOCS2 and NR2E1.

    Mol Syst Biol 2011 7, 486. Gefitinib (ZD1839) purchased from Selleck.

    Effects of shRNA-mediated RECK depletion on EGFR signaling in MEFs. (a) Immunoblotting (IB) of the indicated proteins in wild-type MEFs transduced with the indicated shRNAs and cultured for 48 h in the presence of 0.1% dimethyl sulfoxide (DMSO; vehicle) or 1 mM gefitinib (S1025, Selleck Chemicals,Houston, TX, USA). (b) Immunoprecipitation from whole lysates of cells from panel a with an anti-phosphorylated tyrosine (pTyr) antibody. Precipitates from 500 mg protein in whole cell lysates (upper) or 20 mg protein in whole cell lysates (lower) were analyzed by IB with anti-EGFR antibody.

    Oncogene 2010 30, 737-750. Gefitinib (ZD1839) purchased from Selleck.

  • (B–C) LNCaP (B) and LNCaP-AI (C) cells were transiently transfected with sPLA2-IIa(-800)-Luc (0.5 μg). The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 lM) and Bortezomib (20 μM) without or with EGF (100 ng/ml) for 24 h. Luciferase assay was performed according to a standard protocol with Renilla luciferase as an internal control. Data are presented as the mean (±SD) of duplicate values of a representative experiment that was independently repeated for five times.

    Carcinogenesis 2010 31, 1948–1955 . Gefitinib (ZD1839) purchased from Selleck.

    LNCaP-AI cells were starved in 1% stripped medium for 24 h. The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 μM) and Bortezomib (20 μM) for 24 h. Cell culture medium was collected from each sample and subjected to ELISA for sPLA2-IIa. The condition medium samples were diluted 10 times for ELISA. Average of duplicate samples was converted to nanogram per milliliter against standard curve. The data represent one of five repeated experiments.

     

     

    Carcinogenesis 2010 31, 1948–1955. Gefitinib (ZD1839) purchased from Selleck.

  • Susceptibility of lung cancer cells to cytolytic activity of NK-92 cells after treatment with EGFR inhibitors. Three lung cancer cells were untreated (open circle) or treated with 10 μM erlotinib or gefitinib (black filled square or black filled triangle) for 24 hours. The lung cancer cells were cocultured with NK-92 cells at indicated effector to target ratio (E:T ratio). To determine the specificity of NKG2D-mediated cytolysis of lung cancer cells, NK-92 cells were preincubated with blocking mAb against NKG2D before the assay (gray filled square and triangle) and correspond-ing isotype mAb (open square and triangle).

    J Immunother 2011 34(4), 372-81. Gefitinib (ZD1839) purchased from Selleck.

    Suppression of EGFR signaling by Gefitinib is dose dependent and non- toxic at millimolar concentrations.Results from plaque reduction tests of Hep2 cells infected with VACV(12.5pfu/well) treated with a serial dilution of Gefitinib (1000-0.01 m) and analyzed for (A)proliferation as indicator of cytotoxicity,(B) IC50 of plaque size inhibition, (C)dose dependent inhibition of EGFR-ERK1/2signaling and VACV proteins by Western blot analysis (pEGFR detection was obtained from a different experiment with identicals ettings) and (D)dose dependent inhibition of orthopoxvirus genome replication by real-time PCR.Untreated VACV infectedcells and uninfected cells are shown as controls in the right panel.

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

  • A circular zone of EGFR activated but uninfected cells facilitate virus induced plaque development , efficiently blocked by Gefitinib. (A)Immun of luorescent staining of aplaque reduction tests with Hep2 cells 24,48,72 and 96 h after VACV infection (6.25pfu/chamber). VACV infected cells(green) were visualized by human IgG directed to orthopoxviruses (VIG) detected with a secondary FITC labelled antibody. Uninfected cells with activated EGFR signaling(red) are detected with a secondary Cy3labelled antibody. Positive cells for both , VACV and phosphorylated EGFR are shown in yellow(merge). Cellular nuclei are counterstained with DAPI. (B)Immunofluorescent staining of a plaque reduction test as described for (A) and treated with three different Gefitinib concentrations (1000 nM, 1 nM, and 0.01 nM) . The test was analyzed 96h afterVACV infection.Uninfected cells are shown as controls. Magnification100×.(For interpretation of the references to color in this figure legend , the reader is referred to the web version of the article.)

     

     

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

    The EGF-dependent increased association of PCM1 and AZI1 requires EGFR kinase activity. ZD1839 (Iressa)—EGFR tyrosine kinase inhibitor.

    Mol Biosyst 2011 7, 3223-33. Gefitinib (ZD1839) purchased from Selleck.

  • Inhibition of signaling pathway activation in lung tumor cell lines by kinase inhibitors. Lung tumor cells were cultured in 10% FBS until reaching ∼80% confluence and then the cells were starved in serum-free medium for overnight, followed by 4-hour treatment with the inhibitors. Cell lysates were then prepared and used for determination of the pathway activation signals by the CEER assay.

    Int J Proteomics 2011 215496. Gefitinib (ZD1839) purchased from Selleck.

    Breast cancer cells were pretreated with 100ng/ml EGF for 15 min and then treated with the indicated concentrations of  Gefitinib for 24 hours.

     

     

    2010 Dr. Zhang of Tianjin Medical University. Gefitinib (ZD1839) purchased from Selleck.

  • MTT assay result. 0-500nM Gefitinib treated with H1299, H358, H25 and HCC827 cell lines by 72h, Proliferation rate was tested.

     

     

    2010 Dr. Vicky Tin from University of Hong Kong. Gefitinib (ZD1839) purchased from Selleck.

Purity & Quality Control

Choose Selective EGFR Inhibitors

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Notes:

2. For more details, such as half maximal inhibitory concentrations (IC50s) and working concentrations of each inhibitor, please click on the link of the inhibitor of interest.
3. "+" indicates inhibitory effect. Increased inhibition is marked by a higher "+" designation.
4. Orange "√" refers to compounds which do inhibitory effects on the related isoform, but without specific value.

Biological Activity

Description Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.
Features A potent EGFR tyrosine kinase inhibitor.
Targets
Tyr1173 (NR6W cells) [1] Tyr1173 (NR6wtEGFR cells) [1] Tyr992 (NR6wtEGFR cells) [1] Tyr992 (NR6W cells) [1]
26 nM 37 nM 37 nM 57 nM
In vitro

Gefitinib effectively inhibits all tyrosine phosphorylation sites on EGFR in both the high and low-EGFR-expressing cell lines including NR6, NR6M and NR6W cell lines. The phosphorylation sites Tyr1173 and Tyr992 are less sensitive requiring higher concentrations of Gefitinib for inhibition. Gefitinib effectively blocks the phosphorylation of PLC-γ, with IC50 of 27nM, in NR6W cells. The NR6wtEGFR and NR6M cell lines has low levels of PLC-γ phosphorylation but the level in the NR6M cell line is more resistant to inhibition by Gefitinib with IC50 of 43 nM and 369 nM, respectively. Gefitinib inhibits Akt phosphorylations, with IC50 of 220 and 263nM, in the low-EGFR- and -EGFRvIII-expressing cell lines, respectively. Gefitinib in the dose range from 0.1 to 0.5μM significantly facilitates, rather than abrogates, colony formation of NR6M cells. However, at a concentration of 2 μM Gefitinib completely blocks NR6M colony formation. Gefitinib rapidly and in a dose-dependent manner inhibits EGFR and ERK phosphorylation up to 72 hours after EGF stimulation in both the high- and low-EGFR-expressing cell lines. [1] Gefitinib is the monolayer growth of these EGF-driven untransformed MCF10A cells with an IC50 of 20 nM. [2] The combination of Gefitinib (0.2 μM and 0.5 μM) with irradiation lead to a significant growth inhibition in LoVo cells, compared with radiation alone.. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KB Mk\US5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFvVfVlKSzVyPUCuNFU1KM7:TR?= NEHFRoMyPjVzNkS3Ny=>
NIH3T3 NY\oNI9rTnWwY4Tpc44hSXO|YYm= M2nPcFAvOiEQvF2= M2m4SFIhcA>? MmHESG1UVw>? NF7nepdKdmirYnn0bY9vKG:oIFXHSkB{fGmvdXzheIVlKGi3bXHuJIVz[kJzIHH1eI9xcG:|cHjvdplt[XSrb36ge4l1cCCLQ{WwJI9nKDBwMEG0OEDPxE1? M1r2XVE3PDhyMki0
COS7 Mlz1SpVv[3Srb36gRZN{[Xl? MWO5OkDPxE1? NGTKUWxKdmirYnn0bY9vKG:oIHj1cYFvKEqDS{KgWlYyP0ZibYX0ZY51KGW6cILld5Nm\A>? MUCxO|E4QDd{Mh?=
A549 NXvxXVR2S3m2b4TvfIlkKEG|c3H5 M1\VZlk3KGh? M3;uWGROW09? NFLIdWVKSzVyPUGzMlU6KM7:TR?= M4P1PVE5OzF|OEC3
K562 M363fWN6fG:2b4jpZ{BCe3OjeR?= NX3hUFdOQTZiaB?= NIfLUpZFVVOR NEXZWZZKSzVyPUmuN|Yh|ryP MV6xPFMyOzhyNx?=
MDA-MB-231 MYXDfZRwfG:6aXOgRZN{[Xl? M4LvcVk3KGh? NUnmWHpWTE2VTx?= NVPKOVZJUUN3ME2zOk43OSEQvF2= NF3uNWYyQDNzM{iwOy=>
MOLT4 NGjIVGFEgXSxdH;4bYMhSXO|YYm= NVPjR3c1QTZiaB?= NULFPZhiTE2VTx?= NULSUnpQUUN3ME2xOU4xOiEQvF2= M3vsZlE5OzF|OEC3
MCF7 MoHrR5l1d3SxeHnjJGF{e2G7 M3LueVUxKM7:TR?= MmXQO|IhcA>? NFfuNIRKSzVyPUGyMlA2KM7:TR?= Mn\JNVg4PzF6MUm=
BT474 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWm3NkBp Mn;wRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCEVES3OEBk\WyuczDveoVz\XiycnXzd4lv\yCHUlLiNkB4cXSqIFXDOVAhd2ZiMD6zJO69VQ>? M3Hj[lE6ODJ6NEK0
HN5 NEPUUGdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYe3NkBp M1\HVmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSF61JINmdGy|IH;2[ZJmgHC{ZYPzbY5oKEWJRmKge4l1cCCHQ{WwJI9nKDBwMEGg{txO MorWNVkxOjh2MkS=
BA/F3 MmS5R5l1d3SxeHnjJGF{e2G7 MV[3NkBp MVXDfZRwfG:6aXPpeJkh[WejaX7zeEBud3W|ZTDCRU9HOyClZXzsd{BmgHC{ZYPzbY5oKEWJRmKgUFg2QFJibYX0ZY51KHerdHigTWM2OCCxZjCwMlAyODhizszN M2XlXlE6OjN7MkK5
NIH3T3 NYnBVW9FT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXz6XGRIPzJiaB?= NEfEc2pIem:5dHigbY5pcWKrdHnvckBw\iCvb4Xz[UBPUUhxM2SzJINmdGy|IHX4dJJme3Orbneg[ZJDOiCpZX7lJJdqfGhiSVO1NEBw\iByLkW0JO69VQ>? M4HpXFE6PjZ3M{e3
MCF7 MX M3HPbmZ2dmO2aX;uJGF{e2G7 NYfacXdZOzBibXnu MnTVTY5pcWKrdHnvckBw\iCEQ2LQJIV5eHKnc4Pl[EB4cXSqIFnDOVAhd2ZiMT6xJO69VQ>? NVHr[nh3OTl7M{K5OlA>
A431 MorXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoG1NVAh|ryP MlTVO|IhcA>? MV3HTVUxRTBwNUG0JO69VQ>? NYm3T3ppOTl7Nkm0OlU>
A431 NVPzSnBOU2mwYYPlJGF{e2G7 MYOwMlEh|ryP NVfON45XOSCq MoLSTY5pcWKrdHnvckBw\iCHR1[tbY5lfWOnZDDFS2ZTKHCqb4PwbI9zgWyjdHnvci=> NEHvOGMyQTl4OUS2OS=>
A431 NXTJfXJXU2mwYYPlJGF{e2G7 NHPtcYwyODBizszN NH23SIEyOCCvaX6= NInE[JRRem:ub37n[YQhcW6qaXLpeI9zgSCjY4Tpeol1gSCxZjDFS2YucW6mdXPl[EBGT0[UIIDoc5NxcG:{eXzheIlwdg>? MW[xPVk3QTR4NR?=
Sf9 MmrVT4lv[XOnIFHzd4F6 Mn;YNVAhdWmw M2\tb2ROW09? MXzJcohq[mm2aX;uJI9nKEWJRmKg[ZhxemW|c3XkJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gdoVk\XC2b4KgZZV1d3Cqb4PwbI9zgWyjdHnvckB4cXSqIFnDOVAhd2ZiMD6wN|Mh|ryP M17HN|IxODV4NEK1
NCI-H1975 NXHNc3d4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2jBZlUh|ryP NXvaPGxZPzJiaB?= Mk\mTWM2OD16LkK2JO69VQ>? MV[yNFE2OTZ5MB?=
NCI-H1975 M2jPR2tqdmG|ZTDBd5NigQ>? M1XwclExKM7:TR?= Mlr3TY5pcWKrdHnvckBw\iCHR1\SJIF2fG:yaH;zdIhwenmuYYTpc44> MWqyNFE2OTZ5MB?=
NCI-H1975 NFXjdmlHfW6ldHnvckBCe3OjeR?= NFyxbpIyOCEQvF2= MVLJcohq[mm2aX;uJI9nKEWUQlKyJIF2fG:yaH;zdIhwenmuYYTpc44> Mo\1NlAyPTF4N{C=
NCI-H1975 MXLGeY5kfGmxbjDBd5NigQ>? NULmN5gyPSEQvF2= NYTneYViPDhiaB?= MYfJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGG|c3Xzd4VlKGG|IHHjeIl3[XSrb36gc4Yh[2G|cHHz[U0{ M3TWSFIxOTVzNkew
A375 MnvxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4HZOlQ5KGh? NH;PfppKSzVyPUKwMlU2KM7:TR?= MnjWNlA{ODR3M{e=
SMMC7721 NU\EeWNST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHTuWmk1QCCq MnL0TWM2OD1|Mz6xNkDPxE1? M1;MeFIxOzB2NUO3
DU145 NWfMTWpqS3m2b4TvfIlkKEG|c3H5 MXi1JIQ> NFfXTYxFVVOR MWXJR|UxRTR{LkG3JO69VQ>? NWGyUlFPOjB2Nk[1OVU>
MIAPaCa2 NFXGWYtEgXSxdH;4bYMhSXO|YYm= NXTjb25jPSCm M134WmROW09? NXfzelk4UUN3ME21NE43OiEQvF2= MX6yNFQ3PjV3NR?=
PANC1 MX;DfZRwfG:6aXOgRZN{[Xl? MoXMOUBl NUnaRoRWTE2VTx?= MonoTWM2OD12MD60NkDPxE1? MkPwNlA1PjZ3NUW=
PC9 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mnf2RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDFS2ZTNWSnZnnjbYVvfCCqdX3hckBRSzliY3XscJMhf2m2aDDFR|UxKG:oIECuNFE4KM7:TR?= MUmyNVIxQDhyMh?=
PC9 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MliyRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDndo94fGhvcnXzbZN1[W62IHj1cYFvKFCFOTDj[YxteyCneIDy[ZN{cW6pIFXHSnIhTTd2Nm;BO|UxN1R5OUDNJI12fGGwdDD3bZRpKEWFNUCgc4YhPyEQvF2= MUCyNVIxQDhyMh?=
PC3 MoPwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4nTN|ExKM7:TR?= Mn76O|IhcA>? NFrRR2pFVVOR NFnYfXdKSzVyPUeuOEDPxE1? NEL0XnkzOTN3M{W0Oi=>
MDA-MB-468 NF;tT21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1zoU|ExOCEQvF2= MoLWOFghcA>? MXTEUXNQ NX;LdFltSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJk> NYGyfI1bOjF2Mkm2N|I>
Plasmodium falciparum Mo\xdWhVWyCycn;mbYxqdmdiZn;yJIlvcGmkaYTvdpMhd2ZiUHzhd41w\Gm3bTDmZYxkcXCjcoXtJJBzd2yrZnXyZZRqd25? NGPVS3kzOThzN{C0OS=>
NCI-H1993 NYXoR2VRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3XCclczKGh? MnrBSG1UVw>? M2XnPGlEPTB;MD6xJO69VQ>? MW[yNVkyPjR|Mx?=
NCI-H292 M3LNVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn3KO|IhcA>? MVzEUXNQ Mnz0TWM2OD1yLkCyJO69VQ>? MU[yNVkyPjR|Mx?=
A431 NXHIbpdYU2mwYYPlJGF{e2G7 MkPENkBp M4jpWWROW09? NUf2XIFmUW6qaXLpeIlwdiCxZjDFS2ZTKHCqb4PwbI9zgWyjdHnvckBqdiCHR1[td5RqdXWuYYTl[EBpfW2jbjDBOFMyKHerdHigTWM2OCCxZjCwMlAxOSEQvF2= NI\ZWoIzOjFyMUGzNi=>
MCF7 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3vUN|ExOMLizsznM41N NFnlWVA1QCCq NUTFfWE3TE2VTx?= MUjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkegZ4VtdHNib4\ldoV5eHKnc4PpcochTUeIUjDn[Y5mKHerdHigTWM2OCCxZjCxNk4xPSEQvF2= MoL6NlIyOTlzM{C=
BT474 M{TWeWN6fG:2b4jpZ{BCe3OjeR?= NVnKblBMOTBizszN M3TjUVQ5KGh? NF3qV2pEgXSxdH;4bYNqfHliYXfhbY5{fCC|ZYL1cU1{fGG{dnXkJIh2dWGwIFLUOFc1KGOnbHzz M3TK[lIzOTN6M{C4
MCF7 M3jwSmN6fG:2b4jpZ{BCe3OjeR?= MUGxNEDPxE1? MmnrOFghcA>? NHLtTnBEgXSxdH;4bYNqfHliYXfhbY5{fCC|ZYL1cU1{fGG{dnXkJIh2dWGwIF3DSlch[2WubIOgc5Zmei2neIDy[ZN{cW6pIFXyZmIzKGOnbHzz NITtW|UzOjF|OEOwPC=>
HCC827 MnTrT4lv[XOnIFHzd4F6 M1LtVlAvOTJ3IN88US=> MoXGNlQhcA>? MmrhSG1UVw>? NGr2c49KdmirYnn0bY9vKG:oIFXHSnIh\GWuIFW3OFYuSTd3MDDteZRidnRiWUGwOlgh[XW2b4Doc5NxcG:{eXzheIlwdg>? MUOyNlM{QTN2Mh?=
HCC827 MWDLbY5ie2ViQYPzZZk> M2[yfFAvOTJ3IN88US=> NWjkOGFlOjRiaB?= NHXJWFJFVVOR NEjR[lZKdmirYnn0bY9vKG:oIFXHSnIh\GWuIFW3OFYuSTd3MDDteZRidnRvbXXkbYF1\WRiRYLrJJBpd3OyaH;yfYxifGmxbh?= MY[yNlM{QTN2Mh?=
HCC827 NEjXWJFMcW6jc3WgRZN{[Xl? NFOxcmkxNjF{NTFOwG0> NUXZblZOOjRiaB?= NYjvWpI1TE2VTx?= NXji[FJQUW6qaXLpeIlwdiCxZjDFS2ZTKGSnbDDFO|Q3NUF5NUCgcZV1[W62LX3l[IlifGWmIFHreEBxcG:|cHjvdplt[XSrb36= NVe3b3NzOjJ|M{mzOFI>
A431 NVrqfXJTU2mwYYPlJGF{e2G7 NV7ZdppIOTByIN88US=> MVKzNEBucW5? MkDmTY5pcWKrdHnvckBw\iCHR1\SJIRmemm4ZXSg[pJwdSCqdX3hckBCPDNzIHPlcIwhdWWvYoLhcoUh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBxd2y7LVfseU1VgXJicHjvd5Bpd3K7bHH0bY9vKHerdHigTWM2OCCxZjCxMlA5KM7:TR?= MlHmNlI3QTh5OEK=
H460 M4XnXWN6fG:2b4jpZ{BCe3OjeR?= M3fvWVczKGh? NGi2bJlKSzVyPUWuOVkh|ryP M4LWWlIzPzJ5NES5
HT-29 MV3DfZRwfG:6aXOgRZN{[Xl? NWPNcGE1PzJiaB?= MYLJR|UxRTNwM{[g{txO NFGze3QzOjd{N{S0PS=>
SGC7901 MmO4R5l1d3SxeHnjJGF{e2G7 NIDWeHQ4OiCq M{jEfmlEPTB;MUCuNlYh|ryP NYjrVYdiOjJ5Mke0OFk>
A498 MorIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2fyUGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUS5PEBk\WyuczDlfJBz\XO|aX7nJGVITlJiYX7kJHNTSyC5aYToJGlEPTBib3[gPE43KM7:TR?= MlnpNlI5OTh6NEi=
Calu3 NIfDNYVEgXSxdH;4bYMhSXO|YYm= M1q4XlExKM7:Zz;tUC=> M4LHS|czKGh? NWjWVYlWUUN3ME2xMlYh|ryP MUeyN|EyPjF4OB?=
FADU MoDvR5l1d3SxeHnjJGF{e2G7 NFrN[FkyOCEQvHevcWw> MWq3NkBp NEHlepRKSzVyPUCuNFUh|ryP NHHVVW8zOzFzNkG2PC=>
HCC827 M{nzdGN6fG:2b4jpZ{BCe3OjeR?= NVjoc3BsOTBizsznM41N NFHaTJU4OiCq NV\mcI11UUN3ME2wMlAxOTVizszN NXrtbGxROjNzMU[xOlg>
PC9 NYCxbnNUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mk\GNVAh|rypL33M M1XIblczKGh? NHXvU29KSzVyPUCuNFAyPCEQvF2= M4nOS|I{OTF4MU[4
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IGROV-1 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV\RVHFuUUN3ME2yMlMyOjN5IN88US=> NGXCWGJUSU6JRWK=
JVM-3 MnXBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGL5V|dKSzVyPUKuN|Q3PDNizszN M1;NenNCVkeHUh?=
NCI-H2170 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUH2T|hbUUN3ME2yMlM1Pzh|IN88US=> MYDTRW5ITVJ?
EW-18 MkLyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWK0OWR1UUN3ME2yMlM2PDdzIN88US=> NHWze5BUSU6JRWK=
KU812 NXH2UXNGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1zYbGlEPTB;Mj6zOlc4QSEQvF2= NF\icVBUSU6JRWK=
NY M{Sxe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlXWTWM2OD1{LkO3Nlch|ryP M{PNNnNCVkeHUh?=
HTC-C3 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4XMdmlEPTB;Mj60NlI3PyEQvF2= M4rKZnNCVkeHUh?=
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HH MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUHJR|UxRTJwNEm4OVQh|ryP NYfrdJJuW0GQR1XS
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TE-8 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1;UOmlEPTB;Mj62N|Y2OyEQvF2= NXLI[ZJHW0GQR1XS
MC-IXC NYTkcVhlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUPGUWJuUUN3ME2yMlY1PDV|IN88US=> NESzd4ZUSU6JRWK=
DMS-79 M{fqSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFrCc|hKSzVyPUKuOlkzQDRizszN NWXEbVFrW0GQR1XS
Detroit562 NX:yOpUxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVnueoVXUUN3ME2yMlY6QDR3IN88US=> NEjJU2VUSU6JRWK=
HuP-T4 MoLGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEfpbnVKSzVyPUKuO|I5OThizszN MoLqV2FPT0WU
QIMR-WIL MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4CxRWlEPTB;Mj63OVk2OiEQvF2= MVzTRW5ITVJ?
BB65-RCC NInIcWFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH3aNpBKSzVyPUKuPFA4OTNizszN NWHubHpMW0GQR1XS
PC-3 M2TxS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MojFTWM2OD1{LkizN|E4KM7:TR?= NFnkOVlUSU6JRWK=
OVCAR-3 MortS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIHXco1KSzVyPUKuPFU2QTRizszN NWDsUWE3W0GQR1XS
KYSE-510 M4XJcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWPOe4pvUUN3ME2yMlg3Pzh4IN88US=> NUfYNYpDW0GQR1XS
HC-1 NXHafVVjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2HxXGlEPTB;Mj65OFAyOiEQvF2= M2\3bnNCVkeHUh?=
NCI-H1693 MmCyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{W4fmlEPTB;Mj65OVg6PyEQvF2= MoDRV2FPT0WU
DU-4475 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXXoZW9uUUN3ME2yMlk6ODV{IN88US=> M3TtVnNCVkeHUh?=
COLO-680N MnjrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHTQXoZKSzVyPUOuNFA1QTVizszN NFXFdVJUSU6JRWK=
MN-60 M4TUSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1faRWlEPTB;Mz6wOFk4PCEQvF2= NV;6[Ho2W0GQR1XS
BCPAP NV\qZZVnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3znPWlEPTB;Mz6wPFI6PSEQvF2= MWXTRW5ITVJ?
647-V NGDpSmpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{njVWlEPTB;Mz6xOlIzKM7:TR?= NFqwOpNUSU6JRWK=
HT-1376 MlfrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUDiUHJWUUN3ME2zMlIxPTB4IN88US=> M3\pdXNCVkeHUh?=
NCI-H1793 NG\M[HlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoDHTWM2OD1|LkKxNFk5KM7:TR?= MnnjV2FPT0WU
SW1463 MlXiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGXETYZKSzVyPUOuNlYyQTJizszN MUnTRW5ITVJ?
NCI-H727 M2H1SGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFXxcGVKSzVyPUOuN|g2QDRizszN MnLxV2FPT0WU
LB771-HNC MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVXLRZFLUUN3ME2zMlM6OTJ4IN88US=> MY\TRW5ITVJ?
D-542MG NVLafXpvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4Die2lEPTB;Mz60OFIzOiEQvF2= NUHQfGlZW0GQR1XS
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C8166 M3nGPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV7jZVFrUUN3ME2zMlQ3PTJzIN88US=> Mn\YV2FPT0WU
639-V NFXXe|JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXXUOm1RUUN3ME2zMlU4Pzl{IN88US=> NYjnOnYxW0GQR1XS
TE-1 NF25[JJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{nYWWlEPTB;Mz61PVE3QSEQvF2= MnzzV2FPT0WU
OAW-28 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVfJR|UxRTNwNUm0NlYh|ryP NETFelRUSU6JRWK=
SK-LU-1 M{X0c2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MknRTWM2OD1|Lk[wOlQ2KM7:TR?= MmTqV2FPT0WU
CaR-1 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlfxTWM2OD1|Lk[xNFQ5KM7:TR?= NIW1cJBUSU6JRWK=
A2780 MnKxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3LwcmlEPTB;Mz62OFA5QSEQvF2= NGTmWWhUSU6JRWK=
C2BBe1 NF\jZ2RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3TSW2lEPTB;Mz62OFQxPyEQvF2= MmTVV2FPT0WU
SK-MES-1 NYTDPYs1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmHMTWM2OD1|Lk[0OlE1KM7:TR?= MWjTRW5ITVJ?
PANC-10-05 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYXJR|UxRTNwN{KwO|Ih|ryP M1;RUnNCVkeHUh?=
J-RT3-T3-5 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGT0ZXRKSzVyPUOuO|U1ODZizszN NXnqcG5zW0GQR1XS
ES4 NHzWc3NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4fROWlEPTB;Mz64NlI5PiEQvF2= MUPTRW5ITVJ?
NCI-H28 NWiyV|V3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4D3TGlEPTB;Mz64Nlk3OSEQvF2= NXPZbGVDW0GQR1XS
NCI-H1155 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1jEN2lEPTB;Mz64OFE2QSEQvF2= NGS1e4pUSU6JRWK=
KURAMOCHI NG\kWlNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWXKcGlPUUN3ME2zMlg3ODV4IN88US=> M3:zV3NCVkeHUh?=
NCI-H69 NFLlbmdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVfJR|UxRTNwOUCxO|gh|ryP NGXGUG1USU6JRWK=
IST-MES1 NV7leXlHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEHUcXZKSzVyPUOuPVQxOjlizszN NIjHSZZUSU6JRWK=
NH-12 NH22dXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVLJR|UxRTNwOUSxPFch|ryP MlLZV2FPT0WU
JVM-2 MlPDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlfkTWM2OD1|Lkm0O|UzKM7:TR?= NGj2OXhUSU6JRWK=
EW-16 MlzJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkL1TWM2OD1|Lkm1OVc4KM7:TR?= NFjHNGpUSU6JRWK=
NCI-H441 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWnJR|UxRTNwOUewNVQh|ryP MWDTRW5ITVJ?
RCC10RGB M1fLW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NITBO4JKSzVyPUSuNFEzPDZizszN NYX6Z2FEW0GQR1XS
TCCSUP NV;SNm1HT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MULJR|UxRTRwMUGwOUDPxE1? M1[0fHNCVkeHUh?=
HL-60 MlvFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX\JR|UxRTRwMUKyOFEh|ryP M4\VOHNCVkeHUh?=
EW-11 NETH[VJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{fHbmlEPTB;ND6xN|I3OSEQvF2= NW\VTWpxW0GQR1XS
KARPAS-299 MnjwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYnKTJFLUUN3ME20MlMyODF|IN88US=> NVPBZWt2W0GQR1XS
SNU-C2B MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXXjZodPUUN3ME20MlM1Pjh4IN88US=> Mn3mV2FPT0WU
LU-139 MonVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXfJR|UxRTRwM{eyOFch|ryP M2HIOnNCVkeHUh?=
HCC1937 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NULiOJFlUUN3ME20MlM4QTl|IN88US=> MmrGV2FPT0WU
GR-ST MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmruTWM2OD12LkSzNVgh|ryP M3vWeXNCVkeHUh?=
SK-OV-3 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3O4d2lEPTB;ND60OlA1OiEQvF2= M3nkSXNCVkeHUh?=
A172 NGjxW3hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEm1UGxKSzVyPUSuOVU2OjFizszN M1PBXHNCVkeHUh?=
NB10 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFf0XnFKSzVyPUSuOVk4QDFizszN NHPLO5pUSU6JRWK=
P30-OHK NHfPOIZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NE\qflBKSzVyPUSuPFE6OjlizszN MXvTRW5ITVJ?
KYSE-520 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{CwXmlEPTB;ND64PFAyQCEQvF2= NEHSV|JUSU6JRWK=
KNS-62 NUDFfXI3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml3sTWM2OD12LkmwPFA4KM7:TR?= NWHiRXgxW0GQR1XS
ES1 MoHkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHPhcm9KSzVyPUSuPVYxOzVizszN MVLTRW5ITVJ?
KOSC-2 M3HSSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mm\JTWM2OD12Lkm5PVU6KM7:TR?= MVTTRW5ITVJ?
TE-9 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUfJR|UxRTVwMEG1NlUh|ryP NUm3XY1sW0GQR1XS
NCI-H1838 MnSyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUDJR|UxRTVwMEKwO|Yh|ryP MXvTRW5ITVJ?
KP-N-YN M1PQTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUP5cGhyUUN3ME21MlEzPTlzIN88US=> NIPQO5JUSU6JRWK=
ALL-PO MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXPJR|UxRTVwMkK1PFch|ryP NWTRcnMyW0GQR1XS
NOMO-1 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH;t[lRKSzVyPUWuNlMyPzZizszN MYPTRW5ITVJ?
CGTH-W-1 M1LydWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWG1O453UUN3ME21MlM5PTh2IN88US=> MkP1V2FPT0WU
ATN-1 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX7JR|UxRTVwNEGxPFQh|ryP NWPpfphUW0GQR1XS
AGS MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVzJR|UxRTVwNEm2NVgh|ryP NHfyWnZUSU6JRWK=
SW13 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1TLeWlEPTB;NT61N|I3PSEQvF2= MmTnV2FPT0WU
SW1990 Mmn5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXLJR|UxRTVwNkKyPFUh|ryP M4m1OHNCVkeHUh?=
BFTC-905 M4fxbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXjJR|UxRTVwNkW4O|gh|ryP NFLNOo5USU6JRWK=
RMG-I MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mnj5TWM2OD13Lk[1PVQzKM7:TR?= MVnTRW5ITVJ?
HD-MY-Z M1PxfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2DBUWlEPTB;NT62PFU5OSEQvF2= NHTKSmdUSU6JRWK=
HCC1187 NGDjVmpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{PBXmlEPTB;NT63NVA{OiEQvF2= NXXJRplsW0GQR1XS
HCC2998 Mn3zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXLNdJVLUUN3ME21MlcyQDd5IN88US=> MlTjV2FPT0WU
RT-112 MmfGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUXJR|UxRTVwOUCyOFUh|ryP MlK5V2FPT0WU
CAKI-1 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYfDXXlDUUN3ME22MlAxQTh3IN88US=> NXP1dXl4W0GQR1XS
HDLM-2 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mk\WTWM2OD14LkCxOFg6KM7:TR?= MlXOV2FPT0WU
TE-10 Mn3US5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWPJR|UxRTZwMES4N|Mh|ryP M2rKS3NCVkeHUh?=
KE-37 M4HI[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXTJR|UxRTZwMEeyOVgh|ryP M{\heXNCVkeHUh?=
RCM-1 NYHTbXlIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NULGbVRjUUN3ME22MlA4PzF7IN88US=> M3HjUXNCVkeHUh?=
Mewo M{XaPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWPJR|UxRTZwMUG0PFch|ryP MmXLV2FPT0WU
L-428 MnLvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1fUcmlEPTB;Nj6xPFA{PyEQvF2= NV3MOFAyW0GQR1XS
8-MG-BA Mo\iS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUTmdpF3UUN3ME22MlE6Ozd7IN88US=> NEf4WZNUSU6JRWK=
SK-MEL-28 MnnLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFnkXWhKSzVyPU[uNlA6PjdizszN NWO0b3R5W0GQR1XS
VA-ES-BJ MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MknhTWM2OD14LkOwOFYzKM7:TR?= NX3rXoUzW0GQR1XS
CAL-120 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmnqTWM2OD14LkS1NVYzKM7:TR?= MmW2V2FPT0WU
GAMG MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1Hzc2lEPTB;Nj61NFYzKM7:TR?= M{fCSXNCVkeHUh?=
MSTO-221H MnvxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFPy[YhKSzVyPU[uOVEyPyEQvF2= MmTLV2FPT0WU
GCT MlnKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{jnXmlEPTB;Nj61N|M5PyEQvF2= MW\TRW5ITVJ?
SH-4 MlX5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIqwWJVKSzVyPU[uOVg5PDRizszN NX\r[YpCW0GQR1XS
Calu-3 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXzJR|UxRTZwNkW1Olgh|ryP NUi4[lN1W0GQR1XS
SK-HEP-1 Ml7XS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFPROY1KSzVyPU[uO|E4PTNizszN Mm\FV2FPT0WU
PFSK-1 MnOwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV\mT3VFUUN3ME22Mlc{PDd6IN88US=> MYfTRW5ITVJ?
NB12 MljuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYf1OXRTUUN3ME22Mlc5PDB2IN88US=> MXjTRW5ITVJ?
A388 NVzyZZp5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVnE[oVDUUN3ME22Mlg6PTJizszN NEPFUFdUSU6JRWK=
KLE MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX:0ZlBDUUN3ME22Mlk3PTF4IN88US=> NFXaVllUSU6JRWK=
TE-11 NFfCVmpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWPJR|UxRTdwMES5Olch|ryP MU\TRW5ITVJ?
NCI-H2405 Mmj1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2jPW2lEPTB;Nz6wPFM6OyEQvF2= MXjTRW5ITVJ?
OVCAR-4 NX7neHVHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXjmWIxqUUN3ME23MlE2OzVizszN NXXhOppYW0GQR1XS
KP-N-YS NYm4eoNPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYTJR|UxRTdwMUixOEDPxE1? NIrie3FUSU6JRWK=
NOS-1 MlnNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIPQeI1KSzVyPUeuNlU5OjhizszN NIX5S|ZUSU6JRWK=
DMS-114 MoD1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mmn6TWM2OD15LkK5OlMyKM7:TR?= NInPPWdUSU6JRWK=
NCI-H2030 M3zpdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWHMdYFYUUN3ME23MlM{Pjl6IN88US=> NUH6S|EzW0GQR1XS
HEL MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWHJR|UxRTdwM{e1NlEh|ryP M4\zS3NCVkeHUh?=
CAL-62 NWnvNWN7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{fQWGlEPTB;Nz6zPVg3PiEQvF2= M1rFXXNCVkeHUh?=
COLO-668 NGLhfWpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWjZOYxUUUN3ME23MlQ{QTZ5IN88US=> MlLnV2FPT0WU
SKG-IIIa M4HYOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmD2TWM2OD15LkS1N|gyKM7:TR?= NH;STZdUSU6JRWK=
HCC1419 NHL2dYZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{[zZmlEPTB;Nz60Olg{OyEQvF2= MlfYV2FPT0WU
OS-RC-2 NV3UPXJ{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUPNTHNSUUN3ME23MlQ6QDl2IN88US=> NXzxe4ZRW0GQR1XS
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NCI-H1975 NF3VZ2FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmnRTWM2OD1{MT60OlEh|ryP MVXTRW5ITVJ?
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UACC-62 NFviblFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3LleGlEPTB;MkOuOVg{OyEQvF2= NVv0UJVMW0GQR1XS
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UACC-893 NYPFPJNTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUj4bGRVUUN3ME2yO{43PjNzIN88US=> M2S4fHNCVkeHUh?=
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T98G MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGPxdVRKSzVyPUO3Mlg3OzVizszN MXPTRW5ITVJ?
NB14 M320OGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlHtTWM2OD1|OD60OVQ1KM7:TR?= MVrTRW5ITVJ?
D-423MG M1LObWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3XufGlEPTB;M{iuOFg3QSEQvF2= MlXUV2FPT0WU
NCI-H510A NWn6dJVyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWjGRWxYUUN3ME2zPE43PDVzIN88US=> MULTRW5ITVJ?
NMC-G1 Mn[1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml71TWM2OD1|OD63NlIzKM7:TR?= NUTZdYROW0GQR1XS
SW948 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1fpemlEPTB;M{iuPFk1QSEQvF2= MlKyV2FPT0WU
MFM-223 NY\mXJZ[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVLJR|UxRTN6Lkm0N|Eh|ryP M1m4[HNCVkeHUh?=
PAN1 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUD0SY91UUN3ME2zPE46QTF7IN88US=> MVXTRW5ITVJ?
MZ7-mel NXfp[pViT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2HtVWlEPTB;M{muNVE6QSEQvF2= M{XnZXNCVkeHUh?=
COLO-679 NFvpcHlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NG\INWVKSzVyPUO5Mlk4ODhizszN NWjzSpNoW0GQR1XS
SW872 MojLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXHJR|UxRTRyLkKwN|Eh|ryP NF;4cFNUSU6JRWK=
HT-1197 NYrGNINJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV\3dHRXUUN3ME20NE4zQDZ7IN88US=> MmrtV2FPT0WU
ES6 NH3sZlBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYPJR|UxRTRyLkS1OVIh|ryP M1fKRnNCVkeHUh?=
SNU-387 NHzwN4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUDJR|UxRTRyLkiyN|gh|ryP NYHY[otyW0GQR1XS
T47D NI\JS5lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnjnTWM2OD12MT6wNVkzKM7:TR?= MYnTRW5ITVJ?
G-402 NEXGbGhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX;UXnV{UUN3ME20NU4xQDB7IN88US=> M4LNd3NCVkeHUh?=
MZ1-PC M37oNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXvNfGkyUUN3ME20NU45OSEQvF2= NHPoTWJUSU6JRWK=
SW1417 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIrXSGtKSzVyPUSyMlMxOyEQvF2= MVTTRW5ITVJ?
RERF-LC-MS M4\lXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVXJR|UxRTR{LkWwPFch|ryP MmC5V2FPT0WU
SF539 M2GxNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NELD[nVKSzVyPUSyMlU2OjlizszN MoS3V2FPT0WU
ESS-1 NYnMTJdzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWHJR|UxRTR|LkW1OVgh|ryP MlvuV2FPT0WU
RO82-W-1 MoHzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFvjWIRKSzVyPUSzMlYyPTNizszN M3\iOHNCVkeHUh?=
S-117 NInpWYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWi1XJcyUUN3ME20N{43QTV6IN88US=> M2rk[HNCVkeHUh?=
SW756 M3PLOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXixVpcyUUN3ME20N{45OjR{IN88US=> MmTFV2FPT0WU
CP66-MEL M2flXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGnsRXFKSzVyPUS1MlcyPDNizszN NWHNWHN{W0GQR1XS
H-EMC-SS NWrUVopOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnvlTWM2OD12NT63Olk{KM7:TR?= NW[zWGQ1W0GQR1XS
NCI-H2052 MlLKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4PLR2lEPTB;NEWuPVAzPyEQvF2= NEe4XppUSU6JRWK=
SW684 NH;TU2tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV\JR|UxRTR5LkCwPFEh|ryP NYrnUGExW0GQR1XS
HCC1569 Mk\PS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{m0RWlEPTB;NEeuNVY2QCEQvF2= NYXBXJB6W0GQR1XS
KGN NVjwRldvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MojnTWM2OD12Nz6zN|EyKM7:TR?= NVrYUXFvW0GQR1XS
LCLC-103H M4rWN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2LvS2lEPTB;NEeuOFQxPCEQvF2= MWTTRW5ITVJ?
SW780 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHvTc|JKSzVyPUS3MlY{PTFizszN MYnTRW5ITVJ?
NCI-H1703 M{fyXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHfEWnFKSzVyPUS4MlM{PTJizszN NHr5NXBUSU6JRWK=
HCC1395 NWrIfZVjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWfJR|UxRTR6Lke0N|Ih|ryP NEDKNnBUSU6JRWK=

... Click to View More Cell Line Experimental Data

In vivo Gefitinib (100 mg/kg) improves the anti-tumor effect of radiotherapy in LoVo tumor xenografts. [3] Gefitinib treatment of nude mice bearing established human GEO colon cancer xenografts reveals a reversible dose-dependent inhibition of tumor growth because GEO tumors resumes the growth rate of controls at the end of the treatment. [4]

Protocol

Cell Research:[1]
+ Expand
  • Cell lines: NR6, NR6M and NR6W cells
  • Concentrations: 0-2 μM
  • Incubation Time: 72 hours
  • Method: Exponentially growing cells including NR6, NR6M, NR6M and NR6W cells are seeded in sextuple in 96-well plates at a concentration of 2000 cells/well, allowed to adhere and subsequently washed in PBS and incubated overnight in medium containing 0.5% FCS. Cells are then treated with varying concentrations (0-2 μM) of Gefitinib or the solute control DMSO and EGF. The optimal EGF concentration for inducing proliferation of NR6wtEGFR and NR6W cells has previously been determined and hence NR6wtEGFR and NR6W cells are supplemented with 10 nM and 0.1 nM EGF, respectively. EGF is not added to NR6 and NR6M cells. After 72 hours the amount of cells are measured by performing a MTT proliferation assay.
    (Only for Reference)
Animal Research:[3]
+ Expand
  • Animal Models: Female nude mice (cba nu/nu) aged 8–10 weeks are intra-dermal injected with LoVo cells.
  • Formulation: 0.5% polysorbate
  • Dosages: 100 mg/kg
  • Administration: Once daily by oral administration (0.1 mL/10 g body weight) for 14 days
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 89 mg/mL (199.14 mM)
Ethanol 4 mg/mL (8.95 mM)
Water <1 mg/mL
In vivo 5% DMSO+corn oil 2.5mg/mL

* 1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 446.90
Formula

C22H24ClFN4O3

CAS No. 184475-35-2
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
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    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03050164 Completed Bioequivalence Study Hunan Kelun Pharmaceutical Co., Ltd. September 8, 2016 Phase 1
NCT02856893 Not yet recruiting NSCLC European Organisation for Research and Treatment of Cancer - EORTC|AstraZeneca March 2017 Phase 2
NCT02976116 Recruiting NSCLC Hutchison Medipharma Limited December 2016 Phase 2
NCT02951637 Not yet recruiting Lung Adenocarcinoma Shanghai Chest Hospital|Ruijin Hospital|Changhai Hospital December 2016 Phase 2
NCT02930954 Not yet recruiting Non-small-cell Lung Cancer Caicun Zhou|Tongji University November 2016 Phase 2
NCT02929693 Enrolling by invitation Cancer Shanghai University of Traditional Chinese Medicine|Shanghai Chest Hospital|Shanghai Pulmonary Hospital, Shanghai, China October 2016 Phase 3

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID