Gefitinib (ZD1839)

Catalog No.S1025

Gefitinib (ZD1839) Chemical Structure

Molecular Weight(MW): 446.90

Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.

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Cited by 91 Publications

12 Customer Reviews

  • Perturbation of EGFR by its ligand EGF and gefitinib (ZD-1839 Iressat; inhibits EGFR) produces opposite responses in the predicted EGFR target genes SOCS2 and NR2E1.

    Mol Syst Biol 2011 7, 486. Gefitinib (ZD1839) purchased from Selleck.

    Effects of shRNA-mediated RECK depletion on EGFR signaling in MEFs. (a) Immunoblotting (IB) of the indicated proteins in wild-type MEFs transduced with the indicated shRNAs and cultured for 48 h in the presence of 0.1% dimethyl sulfoxide (DMSO; vehicle) or 1 mM gefitinib (S1025, Selleck Chemicals,Houston, TX, USA). (b) Immunoprecipitation from whole lysates of cells from panel a with an anti-phosphorylated tyrosine (pTyr) antibody. Precipitates from 500 mg protein in whole cell lysates (upper) or 20 mg protein in whole cell lysates (lower) were analyzed by IB with anti-EGFR antibody.

    Oncogene 2010 30, 737-750. Gefitinib (ZD1839) purchased from Selleck.

  • (B–C) LNCaP (B) and LNCaP-AI (C) cells were transiently transfected with sPLA2-IIa(-800)-Luc (0.5 μg). The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 lM) and Bortezomib (20 μM) without or with EGF (100 ng/ml) for 24 h. Luciferase assay was performed according to a standard protocol with Renilla luciferase as an internal control. Data are presented as the mean (±SD) of duplicate values of a representative experiment that was independently repeated for five times.

    Carcinogenesis 2010 31, 1948–1955 . Gefitinib (ZD1839) purchased from Selleck.

    LNCaP-AI cells were starved in 1% stripped medium for 24 h. The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 μM) and Bortezomib (20 μM) for 24 h. Cell culture medium was collected from each sample and subjected to ELISA for sPLA2-IIa. The condition medium samples were diluted 10 times for ELISA. Average of duplicate samples was converted to nanogram per milliliter against standard curve. The data represent one of five repeated experiments.

     

     

    Carcinogenesis 2010 31, 1948–1955. Gefitinib (ZD1839) purchased from Selleck.

  • Susceptibility of lung cancer cells to cytolytic activity of NK-92 cells after treatment with EGFR inhibitors. Three lung cancer cells were untreated (open circle) or treated with 10 μM erlotinib or gefitinib (black filled square or black filled triangle) for 24 hours. The lung cancer cells were cocultured with NK-92 cells at indicated effector to target ratio (E:T ratio). To determine the specificity of NKG2D-mediated cytolysis of lung cancer cells, NK-92 cells were preincubated with blocking mAb against NKG2D before the assay (gray filled square and triangle) and correspond-ing isotype mAb (open square and triangle).

    J Immunother 2011 34(4), 372-81. Gefitinib (ZD1839) purchased from Selleck.

    Suppression of EGFR signaling by Gefitinib is dose dependent and non- toxic at millimolar concentrations.Results from plaque reduction tests of Hep2 cells infected with VACV(12.5pfu/well) treated with a serial dilution of Gefitinib (1000-0.01 m) and analyzed for (A)proliferation as indicator of cytotoxicity,(B) IC50 of plaque size inhibition, (C)dose dependent inhibition of EGFR-ERK1/2signaling and VACV proteins by Western blot analysis (pEGFR detection was obtained from a different experiment with identicals ettings) and (D)dose dependent inhibition of orthopoxvirus genome replication by real-time PCR.Untreated VACV infectedcells and uninfected cells are shown as controls in the right panel.

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

  • A circular zone of EGFR activated but uninfected cells facilitate virus induced plaque development , efficiently blocked by Gefitinib. (A)Immun of luorescent staining of aplaque reduction tests with Hep2 cells 24,48,72 and 96 h after VACV infection (6.25pfu/chamber). VACV infected cells(green) were visualized by human IgG directed to orthopoxviruses (VIG) detected with a secondary FITC labelled antibody. Uninfected cells with activated EGFR signaling(red) are detected with a secondary Cy3labelled antibody. Positive cells for both , VACV and phosphorylated EGFR are shown in yellow(merge). Cellular nuclei are counterstained with DAPI. (B)Immunofluorescent staining of a plaque reduction test as described for (A) and treated with three different Gefitinib concentrations (1000 nM, 1 nM, and 0.01 nM) . The test was analyzed 96h afterVACV infection.Uninfected cells are shown as controls. Magnification100×.(For interpretation of the references to color in this figure legend , the reader is referred to the web version of the article.)

     

     

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

    The EGF-dependent increased association of PCM1 and AZI1 requires EGFR kinase activity. ZD1839 (Iressa)—EGFR tyrosine kinase inhibitor.

    Mol Biosyst 2011 7, 3223-33. Gefitinib (ZD1839) purchased from Selleck.

  • Reduction of GPER expression in HCC1806 cells after treatment with gefitinib. (A) Concentration dependence. HCC1806 cells were treated with 50-500 nM gefitinib for 48 h. GPER was detected by western blotting. (B) Time dependence. HCC1806 cells were treated with 200 nM gefitinib for 24-96 h. ap<0.05 vs. control; bp<0.001 vs. control. Mean values and SE of three independent experiments are shown. (C) Representative western blotting of concentration dependence. (D) Representative western blotting of time dependence

    Oncol Rep, 2017, 37(2):1212-1218. Gefitinib (ZD1839) purchased from Selleck.

    Inhibition of signaling pathway activation in lung tumor cell lines by kinase inhibitors. Lung tumor cells were cultured in 10% FBS until reaching ∼80% confluence and then the cells were starved in serum-free medium for overnight, followed by 4-hour treatment with the inhibitors. Cell lysates were then prepared and used for determination of the pathway activation signals by the CEER assay.

    Int J Proteomics 2011 215496. Gefitinib (ZD1839) purchased from Selleck.

  • Breast cancer cells were pretreated with 100ng/ml EGF for 15 min and then treated with the indicated concentrations of  Gefitinib for 24 hours.

     

     

    2010 Dr. Zhang of Tianjin Medical University. Gefitinib (ZD1839) purchased from Selleck.

    MTT assay result. 0-500nM Gefitinib treated with H1299, H358, H25 and HCC827 cell lines by 72h, Proliferation rate was tested.

     

     

    2010 Dr. Vicky Tin from University of Hong Kong. Gefitinib (ZD1839) purchased from Selleck.

Purity & Quality Control

Choose Selective EGFR Inhibitors

Biological Activity

Description Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.
Features A potent EGFR tyrosine kinase inhibitor.
Targets
Tyr1173 (NR6W cells) [1] Tyr1173 (NR6wtEGFR cells) [1] Tyr992 (NR6wtEGFR cells) [1] Tyr992 (NR6W cells) [1]
26 nM 37 nM 37 nM 57 nM
In vitro

Gefitinib effectively inhibits all tyrosine phosphorylation sites on EGFR in both the high and low-EGFR-expressing cell lines including NR6, NR6M and NR6W cell lines. The phosphorylation sites Tyr1173 and Tyr992 are less sensitive requiring higher concentrations of Gefitinib for inhibition. Gefitinib effectively blocks the phosphorylation of PLC-γ, with IC50 of 27nM, in NR6W cells. The NR6wtEGFR and NR6M cell lines has low levels of PLC-γ phosphorylation but the level in the NR6M cell line is more resistant to inhibition by Gefitinib with IC50 of 43 nM and 369 nM, respectively. Gefitinib inhibits Akt phosphorylations, with IC50 of 220 and 263nM, in the low-EGFR- and -EGFRvIII-expressing cell lines, respectively. Gefitinib in the dose range from 0.1 to 0.5μM significantly facilitates, rather than abrogates, colony formation of NR6M cells. However, at a concentration of 2 μM Gefitinib completely blocks NR6M colony formation. Gefitinib rapidly and in a dose-dependent manner inhibits EGFR and ERK phosphorylation up to 72 hours after EGF stimulation in both the high- and low-EGFR-expressing cell lines. [1] Gefitinib is the monolayer growth of these EGF-driven untransformed MCF10A cells with an IC50 of 20 nM. [2] The combination of Gefitinib (0.2 μM and 0.5 μM) with irradiation lead to a significant growth inhibition in LoVo cells, compared with radiation alone.. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KB NY[3XXU2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF:5dIdKSzVyPUCuNFU1KM7:TR?= M3zURVE3PTF4NEez
NIH3T3 MUHGeY5kfGmxbjDBd5NigQ>? MUGwMlIh|ryP NFnDUHQzKGh? NF\nb|BFVVOR Mlu5TY5pcWKrdHnvckBw\iCHR1[gd5RqdXWuYYTl[EBpfW2jbjDldoJDOSCjdYTvdIhwe3Cqb4L5cIF1cW:wIIfpeIghUUN3MDDv[kAxNjBzNESg{txO MV2xOlQ5ODJ6NB?=
COS7 M{e4PWZ2dmO2aX;uJGF{e2G7 NYPxVlh3QTZizszN NFyxfphKdmirYnn0bY9vKG:oIHj1cYFvKEqDS{KgWlYyP0ZibYX0ZY51KGW6cILld5Nm\A>? M4TnXFE4OTd6N{Ky
A549 NHrX[2REgXSxdH;4bYMhSXO|YYm= NETUU5Q6PiCq MYnEUXNQ M2XuWmlEPTB;MUOuOVkh|ryP NVPNfWR[OTh|MUO4NFc>
K562 MoPsR5l1d3SxeHnjJGF{e2G7 NWjwVZFbQTZiaB?= MYnEUXNQ MVnJR|UxRTlwM{[g{txO NXH2XmdKOTh|MUO4NFc>
MDA-MB-231 MXrDfZRwfG:6aXOgRZN{[Xl? MWm5OkBp MWTEUXNQ NWK5PIpvUUN3ME2zOk43OSEQvF2= MX[xPFMyOzhyNx?=
MOLT4 NWLqSJVGS3m2b4TvfIlkKEG|c3H5 NHH2eno6PiCq NUDtR5RHTE2VTx?= NFLRN3hKSzVyPUG1MlAzKM7:TR?= M4TiPVE5OzF|OEC3
MCF7 MWrDfZRwfG:6aXOgRZN{[Xl? NIDaPFc2OCEQvF2= M3[xNVczKGh? NIDZT5BKSzVyPUGyMlA2KM7:TR?= M3jnRVE5PzdzOEG5
BT474 MoL5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVi3NkBp Ml\lRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCEVES3OEBk\WyuczDveoVz\XiycnXzd4lv\yCHUlLiNkB4cXSqIFXDOVAhd2ZiMD6zJO69VQ>? M1f1[lE6ODJ6NEK0
HN5 NHPQR4VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUK3NkBp MnjORY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKTkWgZ4VtdHNib4\ldoV5eHKnc4PpcochTUeIUjD3bZRpKEWFNUCgc4YhOC5yMTFOwG0> NEe3ZYYyQTB{OESyOC=>
BA/F3 MV;DfZRwfG:6aXOgRZN{[Xl? MnzxO|IhcA>? Mof6R5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgRmEwTjNiY3XscJMh\XiycnXzd4lv\yCHR1\SJGw5PTiUIH31eIFvfCC5aYToJGlEPTBib3[gNE4xOTB6IN88US=> MXWxPVI{QTJ{OR?=
NIH3T3 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2ji[FczKGh? MnvuS5Jwf3SqIHnubIljcXSrb36gc4YhdW:3c3WgUmlJNzOWMzDj[YxteyCneIDy[ZN{cW6pIHXyRlIh\2WwZTD3bZRpKEmFNUCgc4YhOC53NDFOwG0> MX[xPVY3PTN5Nx?=
MCF7 MX NG\zTnZHfW6ldHnvckBCe3OjeR?= Ml6wN|AhdWmw M3TGbWlvcGmkaYTpc44hd2ZiQlPSVEBmgHC{ZYPz[YQhf2m2aDDJR|UxKG:oIEGuNUDPxE1? MnzWNVk6OzJ7NkC=
A431 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MofENVAh|ryP NELv[Y44OiCq NEPTV4ZIUTVyPUCuOVE1KM7:TR?= MYOxPVk3QTR4NR?=
A431 M{L6O2tqdmG|ZTDBd5NigQ>? M3WxN|AvOSEQvF2= MnnpNUBp M4jqZ2lvcGmkaYTpc44hd2ZiRVfGMYlv\HWlZXSgSWdHWiCyaH;zdIhwenmuYYTpc44> MXWxPVk3QTR4NR?=
A431 MmfVT4lv[XOnIFHzd4F6 MnfyNVAxKM7:TR?= NVrzRYFYOTBibXnu MorpVJJwdG:wZ3XkJIlvcGmkaYTvdpkh[WO2aY\peJkhd2ZiRVfGMYlv\HWlZXSgSWdHWiCyaH;zdIhwenmuYYTpc44> NGP2b44yQTl4OUS2OS=>
Sf9 NVLBV4l7U2mwYYPlJGF{e2G7 MWmxNEBucW5? NUjaU5hYTE2VTx?= M2XnNGlvcGmkaYTpc44hd2ZiRVfGVkBmgHC{ZYPz[YQh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBz\WOncITvdkBifXSxcHjvd5Bpd3K7bHH0bY9vKHerdHigTWM2OCCxZjCwMlA{OyEQvF2= M2\SS|IxODV4NEK1
NCI-H1975 NHzjfmVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MofrOUDPxE1? MoDNO|IhcA>? NH7ZdIFKSzVyPUiuNlYh|ryP MYSyNFE2OTZ5MB?=
NCI-H1975 NWi3fmVJU2mwYYPlJGF{e2G7 NVLKO|h[OTBizszN M3PjcWlvcGmkaYTpc44hd2ZiRVfGVkBifXSxcHjvd5Bpd3K7bHH0bY9v MVmyNFE2OTZ5MB?=
NCI-H1975 MVjGeY5kfGmxbjDBd5NigQ>? M3y3[FExKM7:TR?= MUfJcohq[mm2aX;uJI9nKEWUQlKyJIF2fG:yaH;zdIhwenmuYYTpc44> NV;5To5XOjBzNUG2O|A>
NCI-H1975 NVvodIlUTnWwY4Tpc44hSXO|YYm= MWG1JO69VQ>? M4TGdFQ5KGh? NHfrR3NKdmS3Y4Tpc44hd2ZiYYDvdJRwe2m|IHHzd4V{e2WmIHHzJIFkfGm4YYTpc44hd2ZiY3HzdIF{\S1| MXmyNFE2OTZ5MB?=
A375 MoS5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1vHPFQ5KGh? Mk\kTWM2OD1{MD61OUDPxE1? NVnZPIJ4OjB|MES1N|c>
SMMC7721 M{TDUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXvrO2JQPDhiaB?= M{LGeWlEPTB;M{OuNVIh|ryP M4mwZVIxOzB2NUO3
DU145 MUnDfZRwfG:6aXOgRZN{[Xl? M2\VSlUh\A>? Mn\JSG1UVw>? MWnJR|UxRTR{LkG3JO69VQ>? MlXpNlA1PjZ3NUW=
MIAPaCa2 M3fuRmN6fG:2b4jpZ{BCe3OjeR?= M{nNT|Uh\A>? MXXEUXNQ MYDJR|UxRTVyLk[yJO69VQ>? NWrnSIs2OjB2Nk[1OVU>
PANC1 MYLDfZRwfG:6aXOgRZN{[Xl? MX[1JIQ> NEX2eG9FVVOR NFH4b3JKSzVyPUSwMlQzKM7:TR?= NXPUNnJ2OjB2Nk[1OVU>
PC9 MnTlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NW\4dHNSSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBGT0[ULXTl[olkcWWwdDDoeY1idiCSQ{mgZ4VtdHNid3n0bEBGSzVyIH;mJFAvODF5IN88US=> M1PzXVIyOjB6OECy
PC9 MofWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkHXRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDndo94fGhvcnXzbZN1[W62IHj1cYFvKFCFOTDj[YxteyCneIDy[ZN{cW6pIFXHSnIhTTd2Nm;BO|UxN1R5OUDNJI12fGGwdDD3bZRpKEWFNUCgc4YhPyEQvF2= M3HtdlIyOjB6OECy
PC3 NXm3e|lLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3LMSVExKM7:TR?= NIjIeVI4OiCq NFPuZVFFVVOR NFrrO2xKSzVyPUeuOEDPxE1? NVzPXY5OOjF|NUO1OFY>
MDA-MB-468 NVKzSXFNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2LaXlExOCEQvF2= M1\yOlQ5KGh? MXnEUXNQ NHPh[HVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fS=> NXHkS2ljOjF2Mkm2N|I>
Plasmodium falciparum NGThWXNyUFSVIIDyc4ZqdGmwZzDmc5IhcW6qaXLpeI9zeyCxZjDQcIF{dW:maYXtJIZidGOrcHHyeY0heHKxbHnm[ZJifGmxbh?= M2PxVlIyQDF5MES1
NCI-H1993 M2q4Nmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3W3XFczKGh? MXHEUXNQ NHHx[ohKSzVyPUCuNUDPxE1? NG\1[oEzOTlzNkSzNy=>
NCI-H292 MkPKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml\XO|IhcA>? M4nUN2ROW09? MlXyTWM2OD1yLkCyJO69VQ>? MWeyNVkyPjR|Mx?=
A431 Ml[0T4lv[XOnIFHzd4F6 Mn\ZNkBp NUO3OnV{TE2VTx?= NIfSXm5KdmirYnn0bY9vKG:oIFXHSnIheGixc4Doc5J6dGG2aX;uJIlvKEWJRj3zeIlufWyjdHXkJIh2dWGwIFG0N|Ehf2m2aDDJR|UxKG:oIECuNFAyKM7:TR?= MlrzNlIyODFzM{K=
MCF7 M{fmbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWe4NHVwOTBywrFOwIcwdUx? M2DSdVQ5KGh? Mln5SG1UVw>? MmTIRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPQ1[3JINmdGy|IH;2[ZJmgHC{ZYPzbY5oKEWJRmKg[4Vv\SC5aYToJGlEPTBib3[gNVIvODVizszN M3HiPFIzOTF7MUOw
BT474 NHS0NFREgXSxdH;4bYMhSXO|YYm= M{HFbFExKM7:TR?= NWnYZYF5PDhiaB?= M{K1NWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KHOncoXtMZN1[XK4ZXSgbJVu[W5iQmS0O|Qh[2WubIO= M4H3SFIzOTN6M{C4
MCF7 NFHId4REgXSxdH;4bYMhSXO|YYm= MoDVNVAh|ryP MXK0PEBp NFLMVWJEgXSxdH;4bYNqfHliYXfhbY5{fCC|ZYL1cU1{fGG{dnXkJIh2dWGwIF3DSlch[2WubIOgc5Zmei2neIDy[ZN{cW6pIFXyZmIzKGOnbHzz MWGyNlE{QDNyOB?=
HCC827 NHvQ[oNMcW6jc3WgRZN{[Xl? NYLQbVlMOC5zMkWg{txO M{LZWVI1KGh? NIHXSm9FVVOR M4jHRmlvcGmkaYTpc44hd2ZiRVfGVkBl\WxiRUe0Ok1CPzVyIH31eIFvfCC\MUC2PEBifXSxcHjvd5Bpd3K7bHH0bY9v NXTFdZhTOjJ|M{mzOFI>
HCC827 MUnLbY5ie2ViQYPzZZk> M4\NUVAvOTJ3IN88US=> MVeyOEBp MmXUSG1UVw>? M4XTPGlvcGmkaYTpc44hd2ZiRVfGVkBl\WxiRUe0Ok1CPzVyIH31eIFvfC2vZXTpZZRm\CCHcnugdIhwe3Cqb4L5cIF1cW:w MVGyNlM{QTN2Mh?=
HCC827 M2LjfWtqdmG|ZTDBd5NigQ>? NVzmNXc3OC5zMkWg{txO M4\3ZVI1KGh? NXfTSZZITE2VTx?= MkjETY5pcWKrdHnvckBw\iCHR1\SJIRmdCCHN{S2MWE4PTBibYX0ZY51NW2nZHnheIVlKEGtdDDwbI9{eGixconsZZRqd25? NWnLOoJkOjJ|M{mzOFI>
A431 MWfLbY5ie2ViQYPzZZk> Mk\3NVAxKM7:TR?= NFLlPVQ{OCCvaX6= NUHOTpBDUW6qaXLpeIlwdiCxZjDFS2ZTKGSncnn2[YQh\nKxbTDoeY1idiCDNEOxJINmdGxibXXtZpJidmViYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCyb3z5MWdtfS2WeYKgdIhwe3Cqb4L5cIF1cW:wIIfpeIghUUN3MDDv[kAyNjB6IN88US=> MXeyNlY6QDd6Mh?=
H460 NIP6WXJEgXSxdH;4bYMhSXO|YYm= NILnUpc4OiCq NGXQVmVKSzVyPUWuOVkh|ryP MmLUNlI4Ojd2NEm=
HT-29 MVrDfZRwfG:6aXOgRZN{[Xl? MnflO|IhcA>? MYPJR|UxRTNwM{[g{txO MnnONlI4Ojd2NEm=
SGC7901 M1rwXWN6fG:2b4jpZ{BCe3OjeR?= MU[3NkBp NH3JToZKSzVyPUGwMlI3KM7:TR?= NY\vOoh7OjJ5Mke0OFk>
A498 NHjNcHFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEF2OUigZ4VtdHNiZYjwdoV{e2mwZzDFS2ZTKGGwZDDTVmMhf2m2aDDJR|UxKG:oIEiuOkDPxE1? NXPPfJI{OjJ6MUi4OFg>
Calu3 MoSxR5l1d3SxeHnjJGF{e2G7 M3\JVlExKM7:Zz;tUC=> NIqwVIw4OiCq MVnJR|UxRTFwNjFOwG0> MnywNlMyOTZzNki=
FADU NW\NRW5qS3m2b4TvfIlkKEG|c3H5 MnXzNVAh|rypL33M MVi3NkBp M1XSSmlEPTB;MD6wOUDPxE1? MXOyN|EyPjF4OB?=
HCC827 NY\0PVl[S3m2b4TvfIlkKEG|c3H5 NWfBfGhPOTBizsznM41N NXnac5NiPzJiaB?= Ml;0TWM2OD1yLkCwNVUh|ryP NFfKenIzOzFzNkG2PC=>
PC9 M{PFWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXmxNEDPxGdxbVy= M360[|czKGh? MljFTWM2OD1yLkCwNVQh|ryP MlLWNlMyOTZzNki=
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NCI-H2170 NHrrXolIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NImxemZKSzVyPUKuN|Q4QDNizszN M{\4VHNCVkeHUh?=
EW-18 MmLwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{f2[mlEPTB;Mj6zOVQ4OSEQvF2= Mn7wV2FPT0WU
KU812 NUDEUXpyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mke3TWM2OD1{LkO2O|c6KM7:TR?= MVjTRW5ITVJ?
NY M3Tqd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYfiVYFXUUN3ME2yMlM4OjdizszN MXjTRW5ITVJ?
HTC-C3 NGmzbFdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVz3cnB7UUN3ME2yMlQzOjZ5IN88US=> MnruV2FPT0WU
CFPAC-1 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml;QTWM2OD1{LkSzPFY1KM7:TR?= MUjTRW5ITVJ?
HH M3LoS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHfUfJdKSzVyPUKuOFk5PTRizszN NXe1fJZ1W0GQR1XS
MKN7 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHXaRpJKSzVyPUKuOlI4PDhizszN M3HCOHNCVkeHUh?=
TE-8 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1TrfGlEPTB;Mj62N|Y2OyEQvF2= NFzhepZUSU6JRWK=
MC-IXC M33zeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2nnXmlEPTB;Mj62OFQ2OyEQvF2= M3jIOHNCVkeHUh?=
DMS-79 NXnmNHVLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEfo[5FKSzVyPUKuOlkzQDRizszN NG\tNFVUSU6JRWK=
Detroit562 M{WzWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX;JR|UxRTJwNkm4OFUh|ryP NX;NTph6W0GQR1XS
HuP-T4 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkLCTWM2OD1{LkeyPFE5KM7:TR?= MVvTRW5ITVJ?
QIMR-WIL NEXncGJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2n3NWlEPTB;Mj63OVk2OiEQvF2= M2XCd3NCVkeHUh?=
BB65-RCC NWLpUG9NT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFTsXGFKSzVyPUKuPFA4OTNizszN Mlz6V2FPT0WU
PC-3 NYj0[noxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4PmfGlEPTB;Mj64N|MyPyEQvF2= NHKxWINUSU6JRWK=
OVCAR-3 Mk\OS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUjWcXlkUUN3ME2yMlg2PTl2IN88US=> M4fpT3NCVkeHUh?=
KYSE-510 MoL1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NULPcWs6UUN3ME2yMlg3Pzh4IN88US=> NVf4XGZNW0GQR1XS
HC-1 NH;ycJpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFXxU3pKSzVyPUKuPVQxOTJizszN M4X5d3NCVkeHUh?=
NCI-H1693 M3r6TGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXPJR|UxRTJwOUW4PVch|ryP MoL3V2FPT0WU
DU-4475 NYPScoRFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MY\JR|UxRTJwOUmwOVIh|ryP MUDTRW5ITVJ?
COLO-680N NVHUenJ1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYm5T4U{UUN3ME2zMlAxPDl3IN88US=> NVvSdG85W0GQR1XS
MN-60 MlT0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUHJR|UxRTNwMES5O|Qh|ryP NU\TXYluW0GQR1XS
BCPAP NGjsUlVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{\pWmlEPTB;Mz6wPFI6PSEQvF2= MkGzV2FPT0WU
647-V NULuWmdFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkTqTWM2OD1|LkG2NlIh|ryP MnjHV2FPT0WU
HT-1376 NY\tN4VRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mlm1TWM2OD1|LkKwOVA3KM7:TR?= NF7rOGpUSU6JRWK=
NCI-H1793 NVz0bZlVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkXhTWM2OD1|LkKxNFk5KM7:TR?= M{XHcXNCVkeHUh?=
SW1463 NF;FW|dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4rsV2lEPTB;Mz6yOlE6OiEQvF2= NHPNNZlUSU6JRWK=
NCI-H727 M3L3b2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIO0eGhKSzVyPUOuN|g2QDRizszN NHL5WHdUSU6JRWK=
LB771-HNC NI\IVnJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUHJR|UxRTNwM{mxNlYh|ryP MWPTRW5ITVJ?
D-542MG NVm3N4NUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoXNTWM2OD1|LkS0NlIzKM7:TR?= MkjhV2FPT0WU
NTERA-S-cl-D1 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3u5NmlEPTB;Mz60OFk2PyEQvF2= MULTRW5ITVJ?
C8166 NYTGZmRnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYHJR|UxRTNwNE[1NlEh|ryP NWW5bZBNW0GQR1XS
639-V M3K2[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmDSTWM2OD1|LkW3O|kzKM7:TR?= NG\KbHZUSU6JRWK=
TE-1 M364SGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHjyW3NKSzVyPUOuOVkyPjlizszN MUXTRW5ITVJ?
OAW-28 NXv1WG5FT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlXVTWM2OD1|LkW5OFI3KM7:TR?= M2jreXNCVkeHUh?=
SK-LU-1 M3vPNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmnnTWM2OD1|Lk[wOlQ2KM7:TR?= NUOwcY54W0GQR1XS
CaR-1 M4XzR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4joUWlEPTB;Mz62NVA1QCEQvF2= MlTzV2FPT0WU
A2780 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUPJR|UxRTNwNkSwPFkh|ryP NHTrWItUSU6JRWK=
C2BBe1 NUL3OW9ZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2DFOGlEPTB;Mz62OFQxPyEQvF2= MnuxV2FPT0WU
SK-MES-1 M4S0dGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml;TTWM2OD1|Lk[0OlE1KM7:TR?= M1;xUnNCVkeHUh?=
PANC-10-05 M{LjSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MljTTWM2OD1|LkeyNFczKM7:TR?= Moi3V2FPT0WU
J-RT3-T3-5 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1K3fWlEPTB;Mz63OVQxPiEQvF2= NEHYO4hUSU6JRWK=
ES4 M1q5[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV7JR|UxRTNwOEKyPFYh|ryP NXXiXpd4W0GQR1XS
NCI-H28 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmfuTWM2OD1|LkiyPVYyKM7:TR?= MkDEV2FPT0WU
NCI-H1155 M{Xq[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWDJR|UxRTNwOESxOVkh|ryP NIDRfZdUSU6JRWK=
KURAMOCHI MkCzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mki2TWM2OD1|Lki2NFU3KM7:TR?= NVzSR3N5W0GQR1XS
NCI-H69 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWXJR|UxRTNwOUCxO|gh|ryP Mn\qV2FPT0WU
IST-MES1 MmT2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mo\QTWM2OD1|Lkm0NFI6KM7:TR?= M1;iT3NCVkeHUh?=
NH-12 M4KwcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYXJR|UxRTNwOUSxPFch|ryP NV;MZ2FRW0GQR1XS
JVM-2 MnvoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnLxTWM2OD1|Lkm0O|UzKM7:TR?= NH:2NW1USU6JRWK=
EW-16 MkTES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4K0eGlEPTB;Mz65OVU4PyEQvF2= M2\hZ3NCVkeHUh?=
NCI-H441 NHzGSWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUXJR|UxRTNwOUewNVQh|ryP NGL0cIZUSU6JRWK=
RCC10RGB MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVPJR|UxRTRwMEGyOFYh|ryP NIO5c3BUSU6JRWK=
TCCSUP MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUG3NYpYUUN3ME20MlEyODVizszN MXHTRW5ITVJ?
HL-60 NYX5NlhDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlfoTWM2OD12LkGyNlQyKM7:TR?= M2CwOHNCVkeHUh?=
EW-11 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MU\JR|UxRTRwMUOyOlEh|ryP M{HrcHNCVkeHUh?=
KARPAS-299 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX7QPIFMUUN3ME20MlMyODF|IN88US=> NInCPJVUSU6JRWK=
SNU-C2B MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1nIS2lEPTB;ND6zOFY5PiEQvF2= Ml\1V2FPT0WU
LU-139 NVu0UnhkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUHJR|UxRTRwM{eyOFch|ryP MVrTRW5ITVJ?
HCC1937 M{nkemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3PzdGlEPTB;ND6zO|k6OyEQvF2= NUXzdm9MW0GQR1XS
GR-ST MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWnqRmtoUUN3ME20MlQ{OThizszN MWPTRW5ITVJ?
SK-OV-3 M1r5bmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWTJR|UxRTRwNE[wOFIh|ryP M4DyXHNCVkeHUh?=
A172 NFTLe3VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2Lq[2lEPTB;ND61OVUzOSEQvF2= M{DsUHNCVkeHUh?=
NB10 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1nmVWlEPTB;ND61PVc5OSEQvF2= NHXaUHJUSU6JRWK=
P30-OHK Mnv2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWDJR|UxRTRwOEG5Nlkh|ryP Mn\rV2FPT0WU
KYSE-520 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHf2e5FKSzVyPUSuPFgxOThizszN MoDxV2FPT0WU
KNS-62 NETScmhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVjHRlFbUUN3ME20MlkxQDB5IN88US=> M374RnNCVkeHUh?=
ES1 NHHs[3BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUjiUmxJUUN3ME20Mlk3ODN3IN88US=> NX63TFVHW0GQR1XS
KOSC-2 NWDLS5E5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWXJR|UxRTRwOUm5OVkh|ryP NWHjdFMxW0GQR1XS
TE-9 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUO0RVVHUUN3ME21MlAyPTJ3IN88US=> NHTzSItUSU6JRWK=
NCI-H1838 MmrWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoT5TWM2OD13LkCyNFc3KM7:TR?= NXfI[XhTW0GQR1XS
KP-N-YN NVnuVnVET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2LmfmlEPTB;NT6xNlU6OSEQvF2= NGXrW|FUSU6JRWK=
ALL-PO MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmO2TWM2OD13LkKyOVg4KM7:TR?= NYT5VYdbW0GQR1XS
NOMO-1 Ml7DS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHvEWZlKSzVyPUWuNlMyPzZizszN MWHTRW5ITVJ?
CGTH-W-1 M4L1Z2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{K3cWlEPTB;NT6zPFU5PCEQvF2= Mle0V2FPT0WU
ATN-1 NIDwdZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M33IdmlEPTB;NT60NVE5PCEQvF2= MYTTRW5ITVJ?
AGS NIO1ZWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGTtRXFKSzVyPUWuOFk3OThizszN MVrTRW5ITVJ?
SW13 MnuyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NITqW4dKSzVyPUWuOVMzPjVizszN M{TrbnNCVkeHUh?=
SW1990 MkTFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmDTTWM2OD13Lk[yNlg2KM7:TR?= MojtV2FPT0WU
BFTC-905 M3\Qfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4TPO2lEPTB;NT62OVg4QCEQvF2= M4DmOnNCVkeHUh?=
RMG-I NETUS25Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn\aTWM2OD13Lk[1PVQzKM7:TR?= M3XIe3NCVkeHUh?=
HD-MY-Z NWDD[oExT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXXRSZpWUUN3ME21MlY5PThzIN88US=> MmO1V2FPT0WU
HCC1187 M1vGUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkDaTWM2OD13LkexNFMzKM7:TR?= NVvKW5JoW0GQR1XS
HCC2998 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV\POoV1UUN3ME21MlcyQDd5IN88US=> NXLERnNQW0GQR1XS
RT-112 NYflcJNZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmCxTWM2OD13LkmwNlQ2KM7:TR?= NIrvWHRUSU6JRWK=
CAKI-1 NFe1ZpJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3jxWGlEPTB;Nj6wNFk5PSEQvF2= NF3zTIlUSU6JRWK=
HDLM-2 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYPJR|UxRTZwMEG0PFkh|ryP M4LxWnNCVkeHUh?=
TE-10 MkPxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWrkNWdmUUN3ME22MlA1QDN|IN88US=> M1f3SHNCVkeHUh?=
KE-37 NGL6[FFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3fmZmlEPTB;Nj6wO|I2QCEQvF2= Mn[zV2FPT0WU
RCM-1 NV\PUFU3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWHJR|UxRTZwMEe3NVkh|ryP NIDWb4NUSU6JRWK=
Mewo M1PmUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXPaN41{UUN3ME22MlEyPDh5IN88US=> NHW5TlVUSU6JRWK=
L-428 NVy1T4hDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV3JR|UxRTZwMUiwN|ch|ryP M4\6cHNCVkeHUh?=
8-MG-BA MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXizWVNFUUN3ME22MlE6Ozd7IN88US=> NGfUXZdUSU6JRWK=
SK-MEL-28 NWT4S4JYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX;JR|UxRTZwMkC5Olch|ryP MonRV2FPT0WU
VA-ES-BJ M1HFNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnWxTWM2OD14LkOwOFYzKM7:TR?= MUTTRW5ITVJ?
CAL-120 NUThVIM1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX3JR|UxRTZwNEWxOlIh|ryP NFOz[oZUSU6JRWK=
GAMG NITsOIRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWGx[|ZqUUN3ME22MlUxPjJizszN Ml7iV2FPT0WU
MSTO-221H NHvPelRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NILGTGNKSzVyPU[uOVEyPyEQvF2= NWPZR2hZW0GQR1XS
GCT MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVjnTXJNUUN3ME22MlU{Ozh5IN88US=> M2fPNnNCVkeHUh?=
SH-4 NHH0XVlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYHJR|UxRTZwNUi4OFQh|ryP Ml[1V2FPT0WU
Calu-3 M3vxNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MW\JR|UxRTZwNkW1Olgh|ryP Mmi2V2FPT0WU
SK-HEP-1 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2myZ2lEPTB;Nj63NVc2OyEQvF2= NI\ub|RUSU6JRWK=
PFSK-1 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUPGS4d5UUN3ME22Mlc{PDd6IN88US=> NF\EcpdUSU6JRWK=
NB12 M3jvdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnLzTWM2OD14Lke4OFA1KM7:TR?= MVnTRW5ITVJ?
A388 NVTRRVd{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVLJR|UxRTZwOEm1NkDPxE1? M1X5eXNCVkeHUh?=
KLE NVHnVIFQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYDRT29[UUN3ME22Mlk3PTF4IN88US=> MnXJV2FPT0WU
TE-11 M4\0[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWflV5VHUUN3ME23MlA1QTZ5IN88US=> MmflV2FPT0WU
NCI-H2405 NITycIxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEHiTpBKSzVyPUeuNFg{QTNizszN MmPDV2FPT0WU
OVCAR-4 M130S2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYjJR|UxRTdwMUWzOUDPxE1? MYrTRW5ITVJ?
KP-N-YS NY\vUVFST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYXkNWlIUUN3ME23MlE5OTRizszN NUj3SZBQW0GQR1XS
NOS-1 MmLqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVf4S4RxUUN3ME23MlI2QDJ6IN88US=> MV;TRW5ITVJ?
DMS-114 M37EdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoHKTWM2OD15LkK5OlMyKM7:TR?= M37sNXNCVkeHUh?=
NCI-H2030 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2\CV2lEPTB;Nz6zN|Y6QCEQvF2= MYnTRW5ITVJ?
HEL NWXSbVI6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEXaNYtKSzVyPUeuN|c2OjFizszN NIjnSVhUSU6JRWK=
CAL-62 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3nDc2lEPTB;Nz6zPVg3PiEQvF2= M3vzR3NCVkeHUh?=
COLO-668 Ml;TS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYq0fJJ6UUN3ME23MlQ{QTZ5IN88US=> MlXIV2FPT0WU
SKG-IIIa NEn2[YlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUDZRllRUUN3ME23MlQ2OzhzIN88US=> NVjCfHFTW0GQR1XS
HCC1419 M4L6Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHq2cXhKSzVyPUeuOFY5OzNizszN MkfzV2FPT0WU
OS-RC-2 NHPjRpJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFTpfYNKSzVyPUeuOFk5QTRizszN NGLSVI9USU6JRWK=
COLO-829 NXXMWVVQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUPPUVJYUUN3ME23MlY3PDZ|IN88US=> MXHTRW5ITVJ?
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NB14 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVzGXlU4UUN3ME2zPE41PTR2IN88US=> MmfiV2FPT0WU
D-423MG Mo\lS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NU\4[m5rUUN3ME2zPE41QDZ7IN88US=> Mn7zV2FPT0WU
NCI-H510A M17YS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn60TWM2OD1|OD62OFUyKM7:TR?= M1;iSXNCVkeHUh?=
NMC-G1 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIPTTZpKSzVyPUO4MlczOjJizszN Mnu0V2FPT0WU
SW948 M{\kNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYfIc5lWUUN3ME2zPE45QTR7IN88US=> NHfqNGVUSU6JRWK=
MFM-223 NFfvVXJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWrJR|UxRTN6Lkm0N|Eh|ryP NFT2XWpUSU6JRWK=
PAN1 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEH6OoxKSzVyPUO4Mlk6OTlizszN M4fDZnNCVkeHUh?=
MZ7-mel MoXvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVXwZ5lUUUN3ME2zPU4yOTl7IN88US=> NInzOpFUSU6JRWK=
COLO-679 M3frdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFu0elhKSzVyPUO5Mlk4ODhizszN NYDuRpFPW0GQR1XS
SW872 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGnSN4dKSzVyPUSwMlIxOzFizszN M33PR3NCVkeHUh?=
HT-1197 M1PIRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYrRZnBpUUN3ME20NE4zQDZ7IN88US=> MnzGV2FPT0WU
ES6 MoPSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{HaZmlEPTB;NECuOFU2OiEQvF2= MlS3V2FPT0WU
SNU-387 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mnu1TWM2OD12MD64NlM5KM7:TR?= MlyxV2FPT0WU
T47D M3TnRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWPJR|UxRTRzLkCxPVIh|ryP NV\DZ4R{W0GQR1XS
G-402 NFiyN2NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoPNTWM2OD12MT6wPFA6KM7:TR?= NUHIT2lrW0GQR1XS
MZ1-PC M3T1WGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4PjbGlEPTB;NEGuPFEh|ryP NUG2fFIyW0GQR1XS
SW1417 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHr3bo5KSzVyPUSyMlMxOyEQvF2= NITIcW1USU6JRWK=
RERF-LC-MS NWT5PYtbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXToOlNnUUN3ME20Nk42ODh5IN88US=> MXjTRW5ITVJ?
SF539 M3LSbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXzJR|UxRTR{LkW1Nlkh|ryP M2XDXXNCVkeHUh?=
ESS-1 NGPxR|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEC4UplKSzVyPUSzMlU2PThizszN NGnicmtUSU6JRWK=
RO82-W-1 NFruSodIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2juWWlEPTB;NEOuOlE2OyEQvF2= M{\ieXNCVkeHUh?=
S-117 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWDVSpBkUUN3ME20N{43QTV6IN88US=> MkPrV2FPT0WU
SW756 NWT4VG5bT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV\JR|UxRTR|LkiyOFIh|ryP NFvZem1USU6JRWK=
CP66-MEL MoLQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkfBTWM2OD12NT63NVQ{KM7:TR?= MYfTRW5ITVJ?
H-EMC-SS MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVXXVW5jUUN3ME20OU44Pjl|IN88US=> NHLUOYVUSU6JRWK=
NCI-H2052 MnHUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX;Y[WFCUUN3ME20OU46ODJ5IN88US=> MofnV2FPT0WU
SW684 MnfSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIrMcXpKSzVyPUS3MlAxQDFizszN MnjEV2FPT0WU
HCC1569 NFywW4xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXHJR|UxRTR5LkG2OVgh|ryP M1\ob3NCVkeHUh?=
KGN MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnvUTWM2OD12Nz6zN|EyKM7:TR?= NXvUW4d[W0GQR1XS
LCLC-103H MmrSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1\ySmlEPTB;NEeuOFQxPCEQvF2= MWLTRW5ITVJ?
SW780 NXnzUIJVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2XxfWlEPTB;NEeuOlM2OSEQvF2= MmnGV2FPT0WU
NCI-H1703 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mk\tTWM2OD12OD6zN|UzKM7:TR?= NHXCRphUSU6JRWK=
HCC1395 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVvJR|UxRTR6Lke0N|Ih|ryP MX;TRW5ITVJ?

... Click to View More Cell Line Experimental Data

In vivo Gefitinib (100 mg/kg) improves the anti-tumor effect of radiotherapy in LoVo tumor xenografts. [3] Gefitinib treatment of nude mice bearing established human GEO colon cancer xenografts reveals a reversible dose-dependent inhibition of tumor growth because GEO tumors resumes the growth rate of controls at the end of the treatment. [4]

Protocol

Cell Research:[1]
+ Expand
  • Cell lines: NR6, NR6M and NR6W cells
  • Concentrations: 0-2 μM
  • Incubation Time: 72 hours
  • Method: Exponentially growing cells including NR6, NR6M, NR6M and NR6W cells are seeded in sextuple in 96-well plates at a concentration of 2000 cells/well, allowed to adhere and subsequently washed in PBS and incubated overnight in medium containing 0.5% FCS. Cells are then treated with varying concentrations (0-2 μM) of Gefitinib or the solute control DMSO and EGF. The optimal EGF concentration for inducing proliferation of NR6wtEGFR and NR6W cells has previously been determined and hence NR6wtEGFR and NR6W cells are supplemented with 10 nM and 0.1 nM EGF, respectively. EGF is not added to NR6 and NR6M cells. After 72 hours the amount of cells are measured by performing a MTT proliferation assay.
    (Only for Reference)
Animal Research:[3]
+ Expand
  • Animal Models: Female nude mice (cba nu/nu) aged 8–10 weeks are intra-dermal injected with LoVo cells.
  • Formulation: 0.5% polysorbate
  • Dosages: 100 mg/kg
  • Administration: Once daily by oral administration (0.1 mL/10 g body weight) for 14 days
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 89 mg/mL (199.14 mM)
Ethanol 4 mg/mL (8.95 mM)
Water Insoluble
In vivo Add solvents individually and in order:
1% CMC Na
30mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 446.90
Formula

C22H24ClFN4O3

CAS No. 184475-35-2
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03050164 Completed Bioequivalence Study Hunan Kelun Pharmaceutical Co., Ltd. September 8, 2016 Phase 1
NCT02856893 Not yet recruiting NSCLC European Organisation for Research and Treatment of Cancer - EORTC|AstraZeneca March 2017 Phase 2
NCT02976116 Recruiting NSCLC Hutchison Medipharma Limited December 2016 Phase 2
NCT02951637 Not yet recruiting Lung Adenocarcinoma Shanghai Chest Hospital|Ruijin Hospital|Changhai Hospital December 2016 Phase 2
NCT02930954 Not yet recruiting Non-small-cell Lung Cancer Caicun Zhou|Tongji University November 2016 Phase 2
NCT02929693 Enrolling by invitation Cancer Shanghai University of Traditional Chinese Medicine|Shanghai Chest Hospital|Shanghai Pulmonary Hospital, Shanghai, China October 2016 Phase 3

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    I try to dissolve Gefitinib (ZD1839) in 0.5% methylcellulose+0.2% Tween 80 for in vivo experiments. However, when I prepare the mixture, it forms precipitates. Exists another way to dissolve completely the compound?

  • Answer:

    S1025 can be dissovled in both 5% DMSO+corn oil and 5% DMSO+30% PEG 300+5% Tween 80+ddH2O at 2.5 mg/ml clearly. It dissolves in DMSO not readily, please sonicate and warm it in water bath at about 45 degree, and dissolve it clearly first. Then add other solvent.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID