Gefitinib (ZD1839)

Catalog No.S1025

Gefitinib (ZD1839) Chemical Structure

Molecular Weight(MW): 446.90

Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.

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Cited by 91 Publications

12 Customer Reviews

  • Perturbation of EGFR by its ligand EGF and gefitinib (ZD-1839 Iressat; inhibits EGFR) produces opposite responses in the predicted EGFR target genes SOCS2 and NR2E1.

    Mol Syst Biol 2011 7, 486. Gefitinib (ZD1839) purchased from Selleck.

    Effects of shRNA-mediated RECK depletion on EGFR signaling in MEFs. (a) Immunoblotting (IB) of the indicated proteins in wild-type MEFs transduced with the indicated shRNAs and cultured for 48 h in the presence of 0.1% dimethyl sulfoxide (DMSO; vehicle) or 1 mM gefitinib (S1025, Selleck Chemicals,Houston, TX, USA). (b) Immunoprecipitation from whole lysates of cells from panel a with an anti-phosphorylated tyrosine (pTyr) antibody. Precipitates from 500 mg protein in whole cell lysates (upper) or 20 mg protein in whole cell lysates (lower) were analyzed by IB with anti-EGFR antibody.

    Oncogene 2010 30, 737-750. Gefitinib (ZD1839) purchased from Selleck.

  • (B–C) LNCaP (B) and LNCaP-AI (C) cells were transiently transfected with sPLA2-IIa(-800)-Luc (0.5 μg). The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 lM) and Bortezomib (20 μM) without or with EGF (100 ng/ml) for 24 h. Luciferase assay was performed according to a standard protocol with Renilla luciferase as an internal control. Data are presented as the mean (±SD) of duplicate values of a representative experiment that was independently repeated for five times.

    Carcinogenesis 2010 31, 1948–1955 . Gefitinib (ZD1839) purchased from Selleck.

    LNCaP-AI cells were starved in 1% stripped medium for 24 h. The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 μM) and Bortezomib (20 μM) for 24 h. Cell culture medium was collected from each sample and subjected to ELISA for sPLA2-IIa. The condition medium samples were diluted 10 times for ELISA. Average of duplicate samples was converted to nanogram per milliliter against standard curve. The data represent one of five repeated experiments.

     

     

    Carcinogenesis 2010 31, 1948–1955. Gefitinib (ZD1839) purchased from Selleck.

  • Susceptibility of lung cancer cells to cytolytic activity of NK-92 cells after treatment with EGFR inhibitors. Three lung cancer cells were untreated (open circle) or treated with 10 μM erlotinib or gefitinib (black filled square or black filled triangle) for 24 hours. The lung cancer cells were cocultured with NK-92 cells at indicated effector to target ratio (E:T ratio). To determine the specificity of NKG2D-mediated cytolysis of lung cancer cells, NK-92 cells were preincubated with blocking mAb against NKG2D before the assay (gray filled square and triangle) and correspond-ing isotype mAb (open square and triangle).

    J Immunother 2011 34(4), 372-81. Gefitinib (ZD1839) purchased from Selleck.

    Suppression of EGFR signaling by Gefitinib is dose dependent and non- toxic at millimolar concentrations.Results from plaque reduction tests of Hep2 cells infected with VACV(12.5pfu/well) treated with a serial dilution of Gefitinib (1000-0.01 m) and analyzed for (A)proliferation as indicator of cytotoxicity,(B) IC50 of plaque size inhibition, (C)dose dependent inhibition of EGFR-ERK1/2signaling and VACV proteins by Western blot analysis (pEGFR detection was obtained from a different experiment with identicals ettings) and (D)dose dependent inhibition of orthopoxvirus genome replication by real-time PCR.Untreated VACV infectedcells and uninfected cells are shown as controls in the right panel.

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

  • A circular zone of EGFR activated but uninfected cells facilitate virus induced plaque development , efficiently blocked by Gefitinib. (A)Immun of luorescent staining of aplaque reduction tests with Hep2 cells 24,48,72 and 96 h after VACV infection (6.25pfu/chamber). VACV infected cells(green) were visualized by human IgG directed to orthopoxviruses (VIG) detected with a secondary FITC labelled antibody. Uninfected cells with activated EGFR signaling(red) are detected with a secondary Cy3labelled antibody. Positive cells for both , VACV and phosphorylated EGFR are shown in yellow(merge). Cellular nuclei are counterstained with DAPI. (B)Immunofluorescent staining of a plaque reduction test as described for (A) and treated with three different Gefitinib concentrations (1000 nM, 1 nM, and 0.01 nM) . The test was analyzed 96h afterVACV infection.Uninfected cells are shown as controls. Magnification100×.(For interpretation of the references to color in this figure legend , the reader is referred to the web version of the article.)

     

     

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

    The EGF-dependent increased association of PCM1 and AZI1 requires EGFR kinase activity. ZD1839 (Iressa)—EGFR tyrosine kinase inhibitor.

    Mol Biosyst 2011 7, 3223-33. Gefitinib (ZD1839) purchased from Selleck.

  • Reduction of GPER expression in HCC1806 cells after treatment with gefitinib. (A) Concentration dependence. HCC1806 cells were treated with 50-500 nM gefitinib for 48 h. GPER was detected by western blotting. (B) Time dependence. HCC1806 cells were treated with 200 nM gefitinib for 24-96 h. ap<0.05 vs. control; bp<0.001 vs. control. Mean values and SE of three independent experiments are shown. (C) Representative western blotting of concentration dependence. (D) Representative western blotting of time dependence

    Oncol Rep, 2017, 37(2):1212-1218. Gefitinib (ZD1839) purchased from Selleck.

    Inhibition of signaling pathway activation in lung tumor cell lines by kinase inhibitors. Lung tumor cells were cultured in 10% FBS until reaching ∼80% confluence and then the cells were starved in serum-free medium for overnight, followed by 4-hour treatment with the inhibitors. Cell lysates were then prepared and used for determination of the pathway activation signals by the CEER assay.

    Int J Proteomics 2011 215496. Gefitinib (ZD1839) purchased from Selleck.

  • Breast cancer cells were pretreated with 100ng/ml EGF for 15 min and then treated with the indicated concentrations of  Gefitinib for 24 hours.

     

     

    2010 Dr. Zhang of Tianjin Medical University. Gefitinib (ZD1839) purchased from Selleck.

    MTT assay result. 0-500nM Gefitinib treated with H1299, H358, H25 and HCC827 cell lines by 72h, Proliferation rate was tested.

     

     

    2010 Dr. Vicky Tin from University of Hong Kong. Gefitinib (ZD1839) purchased from Selleck.

Purity & Quality Control

Choose Selective EGFR Inhibitors

Biological Activity

Description Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.
Features A potent EGFR tyrosine kinase inhibitor.
Targets
Tyr1173 (NR6W cells) [1] Tyr1173 (NR6wtEGFR cells) [1] Tyr992 (NR6wtEGFR cells) [1] Tyr992 (NR6W cells) [1]
26 nM 37 nM 37 nM 57 nM
In vitro

Gefitinib effectively inhibits all tyrosine phosphorylation sites on EGFR in both the high and low-EGFR-expressing cell lines including NR6, NR6M and NR6W cell lines. The phosphorylation sites Tyr1173 and Tyr992 are less sensitive requiring higher concentrations of Gefitinib for inhibition. Gefitinib effectively blocks the phosphorylation of PLC-γ, with IC50 of 27nM, in NR6W cells. The NR6wtEGFR and NR6M cell lines has low levels of PLC-γ phosphorylation but the level in the NR6M cell line is more resistant to inhibition by Gefitinib with IC50 of 43 nM and 369 nM, respectively. Gefitinib inhibits Akt phosphorylations, with IC50 of 220 and 263nM, in the low-EGFR- and -EGFRvIII-expressing cell lines, respectively. Gefitinib in the dose range from 0.1 to 0.5μM significantly facilitates, rather than abrogates, colony formation of NR6M cells. However, at a concentration of 2 μM Gefitinib completely blocks NR6M colony formation. Gefitinib rapidly and in a dose-dependent manner inhibits EGFR and ERK phosphorylation up to 72 hours after EGF stimulation in both the high- and low-EGFR-expressing cell lines. [1] Gefitinib is the monolayer growth of these EGF-driven untransformed MCF10A cells with an IC50 of 20 nM. [2] The combination of Gefitinib (0.2 μM and 0.5 μM) with irradiation lead to a significant growth inhibition in LoVo cells, compared with radiation alone.. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KB NWX2Smx6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVnKenRNUUN3ME2wMlA2PCEQvF2= Ml;VNVY2OTZ2N{O=
NIH3T3 M2Xxc2Z2dmO2aX;uJGF{e2G7 MUSwMlIh|ryP MoX3NkBp M3vXdmROW09? NWDRfI5RUW6qaXLpeIlwdiCxZjDFS2Yhe3SrbYXsZZRm\CCqdX3hckBmemKEMTDheZRweGixc4Doc5J6dGG2aX;uJJdqfGhiSVO1NEBw\iByLkCxOFQh|ryP M2XNOlE3PDhyMki0
COS7 NIKxXJpHfW6ldHnvckBCe3OjeR?= NVnxfJY6QTZizszN NGH1dpVKdmirYnn0bY9vKG:oIHj1cYFvKEqDS{KgWlYyP0ZibYX0ZY51KGW6cILld5Nm\A>? M3TXNVE4OTd6N{Ky
A549 MX\DfZRwfG:6aXOgRZN{[Xl? MnXzPVYhcA>? NYPVbG9KTE2VTx?= NHLGOFVKSzVyPUGzMlU6KM7:TR?= MXOxPFMyOzhyNx?=
K562 M1\DSWN6fG:2b4jpZ{BCe3OjeR?= MmrwPVYhcA>? Mk\WSG1UVw>? M3\3SmlEPTB;OT6zOkDPxE1? NGrOPYoyQDNzM{iwOy=>
MDA-MB-231 Mmn6R5l1d3SxeHnjJGF{e2G7 M4HxTFk3KGh? NUTKT2lxTE2VTx?= MWDJR|UxRTN4Lk[xJO69VQ>? NYT5cFdUOTh|MUO4NFc>
MOLT4 NV;4OGU5S3m2b4TvfIlkKEG|c3H5 MWe5OkBp NEL0WoFFVVOR NX;TZ5hUUUN3ME2xOU4xOiEQvF2= M{fBWFE5OzF|OEC3
MCF7 MlnQR5l1d3SxeHnjJGF{e2G7 MnvqOVAh|ryP M1;tcVczKGh? NYL4e3A5UUN3ME2xNk4xPSEQvF2= NWW1c2xwOTh5N{G4NVk>
BT474 NX23RZlMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUL0fmZwPzJiaB?= NIDtb4tCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFLUOFc1KGOnbHzzJI93\XKneIDy[ZN{cW6pIFXSRoIzKHerdHigSWM2OCCxZjCwMlMh|ryP M1vlb|E6ODJ6NEK0
HN5 NU[4PIh[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M16ye|czKGh? MmfBRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKTkWgZ4VtdHNib4\ldoV5eHKnc4PpcochTUeIUjD3bZRpKEWFNUCgc4YhOC5yMTFOwG0> NFq3[XYyQTB{OESyOC=>
BA/F3 MVPDfZRwfG:6aXOgRZN{[Xl? NGflSFE4OiCq Mn;XR5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgRmEwTjNiY3XscJMh\XiycnXzd4lv\yCHR1\SJGw5PTiUIH31eIFvfCC5aYToJGlEPTBib3[gNE4xOTB6IN88US=> NGDPUJAyQTJ|OUKyPS=>
NIH3T3 NV:0NIxbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX23NkBp MnruS5Jwf3SqIHnubIljcXSrb36gc4YhdW:3c3WgUmlJNzOWMzDj[YxteyCneIDy[ZN{cW6pIHXyRlIh\2WwZTD3bZRpKEmFNUCgc4YhOC53NDFOwG0> NXrzXFBTOTl4NkWzO|c>
MCF7 MX MV7GeY5kfGmxbjDBd5NigQ>? MXizNEBucW5? NYS0OJFyUW6qaXLpeIlwdiCxZjDCR3JRKGW6cILld5Nm\CC5aYToJGlEPTBib3[gNU4yKM7:TR?= M1LNfVE6QTN{OU[w
A431 MmCyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWCxNEDPxE1? NXX6Sox1PzJiaB?= NIf2bW9IUTVyPUCuOVE1KM7:TR?= MYmxPVk3QTR4NR?=
A431 NFftc4ZMcW6jc3WgRZN{[Xl? NVfkOWd[OC5zIN88US=> NWXCN5lkOSCq NWmxSnF4UW6qaXLpeIlwdiCxZjDFS2YucW6mdXPl[EBGT0[UIIDoc5NxcG:{eXzheIlwdg>? MorpNVk6Pjl2NkW=
A431 NFPmfWRMcW6jc3WgRZN{[Xl? NUiyTHFKOTByIN88US=> NX3PUWtHOTBibXnu Mlu1VJJwdG:wZ3XkJIlvcGmkaYTvdpkh[WO2aY\peJkhd2ZiRVfGMYlv\HWlZXSgSWdHWiCyaH;zdIhwenmuYYTpc44> MlnRNVk6Pjl2NkW=
Sf9 NEH6e|BMcW6jc3WgRZN{[Xl? MmHFNVAhdWmw M2ntb2ROW09? MnXrTY5pcWKrdHnvckBw\iCHR1\SJIV5eHKnc4Pl[EBie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJJm[2WydH;yJIF2fG:yaH;zdIhwenmuYYTpc44hf2m2aDDJR|UxKG:oIECuNFM{KM7:TR?= M3jaOFIxODV4NEK1
NCI-H1975 Mm\5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWG1JO69VQ>? M1XQdlczKGh? NYnyOmlrUUN3ME24MlI3KM7:TR?= MXyyNFE2OTZ5MB?=
NCI-H1975 NEXVU41McW6jc3WgRZN{[Xl? MnHGNVAh|ryP MVTJcohq[mm2aX;uJI9nKEWJRmKgZZV1d3Cqb4PwbI9zgWyjdHnvci=> NHnlNIIzODF3MU[3NC=>
NCI-H1975 MnOwSpVv[3Srb36gRZN{[Xl? M2DmbFExKM7:TR?= MXTJcohq[mm2aX;uJI9nKEWUQlKyJIF2fG:yaH;zdIhwenmuYYTpc44> NXH3TWt6OjBzNUG2O|A>
NCI-H1975 MVXGeY5kfGmxbjDBd5NigQ>? MVe1JO69VQ>? NES1Znk1QCCq MUXJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGG|c3Xzd4VlKGG|IHHjeIl3[XSrb36gc4Yh[2G|cHHz[U0{ NIXCVowzODF3MU[3NC=>
A375 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3HPSFQ5KGh? NVTZboZsUUN3ME2yNE42PSEQvF2= MkLlNlA{ODR3M{e=
SMMC7721 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{jDe|Q5KGh? M3u3cmlEPTB;M{OuNVIh|ryP NYC3VG9KOjB|MES1N|c>
DU145 NIX6W4REgXSxdH;4bYMhSXO|YYm= Mn34OUBl M1jLRmROW09? M4jnWmlEPTB;NEKuNVch|ryP NF\RT5MzODR4NkW1OS=>
MIAPaCa2 NYXFXWhuS3m2b4TvfIlkKEG|c3H5 NFTXb2E2KGR? NHTyXpdFVVOR NWDqdnduUUN3ME21NE43OiEQvF2= MWOyNFQ3PjV3NR?=
PANC1 MnHiR5l1d3SxeHnjJGF{e2G7 Mk\NOUBl MYHEUXNQ M{fBcWlEPTB;NECuOFIh|ryP M3rpTFIxPDZ4NUW1
PC9 NHrtRXJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH3XV|BCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGVITlJvZHXmbYNq\W62IHj1cYFvKFCFOTDj[YxteyC5aYToJGVEPTBib3[gNE4xOTdizszN M3XTd|IyOjB6OECy
PC9 NHrOZoVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoDSRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDndo94fGhvcnXzbZN1[W62IHj1cYFvKFCFOTDj[YxteyCneIDy[ZN{cW6pIFXHSnIhTTd2Nm;BO|UxN1R5OUDNJI12fGGwdDD3bZRpKEWFNUCgc4YhPyEQvF2= MmXkNlEzODh6MEK=
PC3 NV\uOmtKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFvIWZoyOCEQvF2= NYj6RZk2PzJiaB?= NGHGcHVFVVOR NYLlOXBuUUN3ME23MlQh|ryP MVqyNVM2OzV2Nh?=
MDA-MB-468 NGH0OlVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVOxNFAh|ryP NF\ScJY1QCCq NEG5eIpFVVOR M4CxW2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5 NUO0VXV1OjF2Mkm2N|I>
Plasmodium falciparum M3S3fJFJXFNicILv[oltcW6pIH\vdkBqdmirYnn0c5J{KG:oIGDsZZNud2SrdX2g[oFt[2myYYL1cUBxem:uaX\ldoF1cW:w Mk\NNlE5OTdyNEW=
NCI-H1993 M2XURmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1v1fVczKGh? NUTPXXE4TE2VTx?= Mlf2TWM2OD1yLkGg{txO NGX3dFMzOTlzNkSzNy=>
NCI-H292 M1m3VWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1TK[VczKGh? NHPRNHBFVVOR M4PTc2lEPTB;MD6wNkDPxE1? NWTRSXkzOjF7MU[0N|M>
A431 NWfhTII5U2mwYYPlJGF{e2G7 Ml7vNkBp Mk[xSG1UVw>? M3XEWGlvcGmkaYTpc44hd2ZiRVfGVkBxcG:|cHjvdplt[XSrb36gbY4hTUeILYP0bY12dGG2ZXSgbJVu[W5iQUSzNUB4cXSqIFnDOVAhd2ZiMD6wNFEh|ryP NELaZ2szOjFyMUGzNi=>
MCF7 NV7GZ|JKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnjBNVAxyqEQvHevcWw> NELHZZo1QCCq NFLj[YpFVVOR NVzlVWM2SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJI93\XKneIDy[ZN{cW6pIFXHSnIh\2WwZTD3bZRpKEmFNUCgc4YhOTJwMEWg{txO M4PicVIzOTF7MUOw
BT474 NFr4cGJEgXSxdH;4bYMhSXO|YYm= NVTaSpNVOTBizszN MYe0PEBp MVjDfZRwfG:6aXPpeJkh[WejaX7zeEB{\XK3bT3zeIFzfmWmIHj1cYFvKEKWNEe0JINmdGy| NYrJZ5JGOjJzM{izNFg>
MCF7 M{TLXGN6fG:2b4jpZ{BCe3OjeR?= MmOzNVAh|ryP NWrCd5RFPDhiaB?= MULDfZRwfG:6aXPpeJkh[WejaX7zeEB{\XK3bT3zeIFzfmWmIHj1cYFvKE2FRkegZ4VtdHNib4\ldk1mgHC{ZYPzbY5oKEW{YlKyJINmdGy| NEmxOpIzOjF|OEOwPC=>
HCC827 NFzodoxMcW6jc3WgRZN{[Xl? NVvjfnNnOC5zMkWg{txO MkP6NlQhcA>? Mk[1SG1UVw>? NInMNYdKdmirYnn0bY9vKG:oIFXHSnIh\GWuIFW3OFYuSTd3MDDteZRidnRiWUGwOlgh[XW2b4Doc5NxcG:{eXzheIlwdg>? NFPsdJozOjN|OUO0Ni=>
HCC827 M4HYe2tqdmG|ZTDBd5NigQ>? MkjsNE4yOjVizszN NXzNVXBnOjRiaB?= M3XQUGROW09? NUHFRWViUW6qaXLpeIlwdiCxZjDFS2ZTKGSnbDDFO|Q3NUF5NUCgcZV1[W62LX3l[IlifGWmIFXyb{BxcG:|cHjvdplt[XSrb36= MVuyNlM{QTN2Mh?=
HCC827 NIP3cXJMcW6jc3WgRZN{[Xl? NYOxNWpxOC5zMkWg{txO NWHiXlV6OjRiaB?= MknQSG1UVw>? NVHlSJNwUW6qaXLpeIlwdiCxZjDFS2ZTKGSnbDDFO|Q3NUF5NUCgcZV1[W62LX3l[IlifGWmIFHreEBxcG:|cHjvdplt[XSrb36= MUWyNlM{QTN2Mh?=
A431 MV3LbY5ie2ViQYPzZZk> M2DKVFExOCEQvF2= M3vYXFMxKG2rbh?= MlK1TY5pcWKrdHnvckBw\iCHR1\SJIRmemm4ZXSg[pJwdSCqdX3hckBCPDNzIHPlcIwhdWWvYoLhcoUh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBxd2y7LVfseU1VgXJicHjvd5Bpd3K7bHH0bY9vKHerdHigTWM2OCCxZjCxMlA5KM7:TR?= MlHCNlI3QTh5OEK=
H460 MXXDfZRwfG:6aXOgRZN{[Xl? NH[3WY84OiCq MofaTWM2OD13LkW5JO69VQ>? NFnh[WszOjd{N{S0PS=>
HT-29 NHLJRXBEgXSxdH;4bYMhSXO|YYm= NHvoUIo4OiCq M4\GOmlEPTB;Mz6zOkDPxE1? MUKyNlczPzR2OR?=
SGC7901 M3n0R2N6fG:2b4jpZ{BCe3OjeR?= NYC0dZZjPzJiaB?= M1i3XGlEPTB;MUCuNlYh|ryP NV3YXmt7OjJ5Mke0OFk>
A498 NV\sUnV{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3LZS2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUS5PEBk\WyuczDlfJBz\XO|aX7nJGVITlJiYX7kJHNTSyC5aYToJGlEPTBib3[gPE43KM7:TR?= NEO5W48zOjhzOEi0PC=>
Calu3 MVzDfZRwfG:6aXOgRZN{[Xl? NV;6UWE3OTBizsznM41N M{XB[FczKGh? Mm\STWM2OD1zLk[g{txO M4XB[lI{OTF4MU[4
FADU Ml[xR5l1d3SxeHnjJGF{e2G7 NFjWN5oyOCEQvHevcWw> NVjBe3pIPzJiaB?= MoLETWM2OD1yLkC1JO69VQ>? MYGyN|EyPjF4OB?=
HCC827 MmX0R5l1d3SxeHnjJGF{e2G7 NYrNU|NuOTBizsznM41N NULvfWFnPzJiaB?= NF:zT25KSzVyPUCuNFAyPSEQvF2= MYOyN|EyPjF4OB?=
PC9 MoiwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnK3NVAh|rypL33M M3PlOVczKGh? MUDJR|UxRTBwMECxOEDPxE1? NHrBemYzOzFzNkG2PC=>
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16HBE14o- MU\DfZRwfG:6aXOgRZN{[Xl? MlnyNVAh|ryP NHmxN2c4OiCq MmDTSG1UVw>? MoTvR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gNVZJSkVzNH:tJINmdGy|IHjhdoJwemmwZzD3bYxlKHS7cHWgSWdHWiCjc4Pld5Nm\CCjczDndo94fGhiaX7obYJqfGmxbjD3bZRpKEmFNUCgc4YhOTJwN{Gg{txO Ml7UNlM3Pjh2NEG=
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BT-20 NWX3bW9jT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEfacHVKSzVyPUKuNlY4PDRizszN NV;wcFZ6W0GQR1XS
A498 NUi3cXBuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MonqTWM2OD1{LkK5OFE2KM7:TR?= NIruWo9USU6JRWK=
IGROV-1 M1TUVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mnn4TWM2OD1{LkOxNlM4KM7:TR?= M4PvUnNCVkeHUh?=
JVM-3 NVHNWXo1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUjJR|UxRTJwM{S2OFMh|ryP NGHC[lhUSU6JRWK=
NCI-H2170 M3zDdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MorYTWM2OD1{LkO0O|g{KM7:TR?= NVrVOnM2W0GQR1XS
EW-18 NWH3VXRwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWHtZVVtUUN3ME2yMlM2PDdzIN88US=> MlTmV2FPT0WU
KU812 NXPXfVR3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYfJR|UxRTJwM{[3O|kh|ryP MkLTV2FPT0WU
NY NFW3b4xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWnJR|UxRTJwM{eyO{DPxE1? M{\IU3NCVkeHUh?=
HTC-C3 MkLSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYDJR|UxRTJwNEKyOlch|ryP Ml;wV2FPT0WU
CFPAC-1 NEC0PXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWXJR|UxRTJwNEO4OlQh|ryP MmLyV2FPT0WU
HH MoLvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUDJR|UxRTJwNEm4OVQh|ryP MnzPV2FPT0WU
MKN7 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV63WWY1UUN3ME2yMlYzPzR6IN88US=> NXjI[mg{W0GQR1XS
TE-8 M{T1e2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHzVXIJKSzVyPUKuOlM3PTNizszN NFnzRZpUSU6JRWK=
MC-IXC NUjKWWJET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml75TWM2OD1{Lk[0OFU{KM7:TR?= NHv1fHNUSU6JRWK=
DMS-79 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4DHVmlEPTB;Mj62PVI5PCEQvF2= MnryV2FPT0WU
Detroit562 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3niWWlEPTB;Mj62PVg1PSEQvF2= NWLNWVZlW0GQR1XS
HuP-T4 M3uwd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWK4O21kUUN3ME2yMlczQDF6IN88US=> MV\TRW5ITVJ?
QIMR-WIL MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHzWO|dKSzVyPUKuO|U6PTJizszN NX[zS|drW0GQR1XS
BB65-RCC M3LGW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1PGdmlEPTB;Mj64NFcyOyEQvF2= NUGzN4ZbW0GQR1XS
PC-3 MmrjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXfJR|UxRTJwOEOzNVch|ryP M3\xdHNCVkeHUh?=
OVCAR-3 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1vnbmlEPTB;Mj64OVU6PCEQvF2= NGrRdWNUSU6JRWK=
KYSE-510 MknPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXvJR|UxRTJwOE[3PFYh|ryP NV7lWJRZW0GQR1XS
HC-1 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M13XVGlEPTB;Mj65OFAyOiEQvF2= MYPTRW5ITVJ?
NCI-H1693 MnS1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYrRPZlCUUN3ME2yMlk2QDl5IN88US=> M2DaRnNCVkeHUh?=
DU-4475 NGjSTHNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFn6V|dKSzVyPUKuPVkxPTJizszN NGn2WGZUSU6JRWK=
COLO-680N M3HhUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUPCWI1pUUN3ME2zMlAxPDl3IN88US=> NUD3O5ZzW0GQR1XS
MN-60 MlfxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUPJR|UxRTNwMES5O|Qh|ryP NVfIW4g{W0GQR1XS
BCPAP MmS4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWD5WYFLUUN3ME2zMlA5Ojl3IN88US=> NF;VTGNUSU6JRWK=
647-V Mn3hS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIXDOYhKSzVyPUOuNVYzOiEQvF2= MVjTRW5ITVJ?
HT-1376 NGHj[49Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXLmR|NlUUN3ME2zMlIxPTB4IN88US=> M{\kSnNCVkeHUh?=
NCI-H1793 M4rmU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGT0RYtKSzVyPUOuNlExQThizszN MoT4V2FPT0WU
SW1463 M{S5[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEDjUHFKSzVyPUOuNlYyQTJizszN M{PlZnNCVkeHUh?=
NCI-H727 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn3iTWM2OD1|LkO4OVg1KM7:TR?= M{e5U3NCVkeHUh?=
LB771-HNC NFzob49Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2HFcmlEPTB;Mz6zPVEzPiEQvF2= NW\rb2M1W0GQR1XS
D-542MG NF;Tcm5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M361OmlEPTB;Mz60OFIzOiEQvF2= M4XJTnNCVkeHUh?=
NTERA-S-cl-D1 NUHVOpIzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1vCXmlEPTB;Mz60OFk2PyEQvF2= NHn0dmxUSU6JRWK=
C8166 MlTpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX7JR|UxRTNwNE[1NlEh|ryP NHe3VWRUSU6JRWK=
639-V M3HtOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWn3eVR2UUN3ME2zMlU4Pzl{IN88US=> M1LyPXNCVkeHUh?=
TE-1 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVzJR|UxRTNwNUmxOlkh|ryP M{K0PXNCVkeHUh?=
OAW-28 NHflRnNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXHkNmZEUUN3ME2zMlU6PDJ4IN88US=> NYHUdnh2W0GQR1XS
SK-LU-1 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUjxb3l7UUN3ME2zMlYxPjR3IN88US=> Mo\kV2FPT0WU
CaR-1 NFLjOm9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn;XTWM2OD1|Lk[xNFQ5KM7:TR?= NGDP[ItUSU6JRWK=
A2780 NVjtdm5rT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXywZYliUUN3ME2zMlY1ODh7IN88US=> Mlm1V2FPT0WU
C2BBe1 NF7y[FNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIXEbFdKSzVyPUOuOlQ1ODdizszN M2\EcXNCVkeHUh?=
SK-MES-1 M2XtRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmrETWM2OD1|Lk[0OlE1KM7:TR?= M2C3cXNCVkeHUh?=
PANC-10-05 NW\PSnVXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2DY[mlEPTB;Mz63NlA4OiEQvF2= Mn3SV2FPT0WU
J-RT3-T3-5 M1TobGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{\ROmlEPTB;Mz63OVQxPiEQvF2= NWL1SFVjW0GQR1XS
ES4 M2SwUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVfjNoFEUUN3ME2zMlgzOjh4IN88US=> MmjTV2FPT0WU
NCI-H28 MmnLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3zpcGlEPTB;Mz64Nlk3OSEQvF2= NHzTPGVUSU6JRWK=
NCI-H1155 Ml3WS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoL2TWM2OD1|Lki0NVU6KM7:TR?= MUfTRW5ITVJ?
KURAMOCHI Mk\NS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF7hWoxKSzVyPUOuPFYxPTZizszN Mm[5V2FPT0WU
NCI-H69 Mo[yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NELEOXpKSzVyPUOuPVAyPzhizszN M1PQPXNCVkeHUh?=
IST-MES1 NIr0[2RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml;DTWM2OD1|Lkm0NFI6KM7:TR?= MX;TRW5ITVJ?
NH-12 MmXkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoTDTWM2OD1|Lkm0NVg4KM7:TR?= MX7TRW5ITVJ?
JVM-2 M4K4Z2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3LINmlEPTB;Mz65OFc2OiEQvF2= NIjCb4NUSU6JRWK=
EW-16 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2ToS2lEPTB;Mz65OVU4PyEQvF2= NVnUNlg1W0GQR1XS
NCI-H441 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGTM[5JKSzVyPUOuPVcxOTRizszN NXThVFRsW0GQR1XS
RCC10RGB MorvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUPpb2E{UUN3ME20MlAyOjR4IN88US=> MW\TRW5ITVJ?
TCCSUP M3r6eGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3TVfGlEPTB;ND6xNVA2KM7:TR?= MWfTRW5ITVJ?
HL-60 NYjNN|ZGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3:1XWlEPTB;ND6xNlI1OSEQvF2= NYnTTlNwW0GQR1XS
EW-11 MlfzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYfJR|UxRTRwMUOyOlEh|ryP MVvTRW5ITVJ?
KARPAS-299 NF[we3RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYjJR|UxRTRwM{GwNVMh|ryP M2PiS3NCVkeHUh?=
SNU-C2B MnfqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3;YdmlEPTB;ND6zOFY5PiEQvF2= NW\kUZhCW0GQR1XS
LU-139 M2XsbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoXOTWM2OD12LkO3NlQ4KM7:TR?= MYfTRW5ITVJ?
HCC1937 MlHLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MULJR|UxRTRwM{e5PVMh|ryP Mn76V2FPT0WU
GR-ST NIHUSIxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MULJR|UxRTRwNEOxPEDPxE1? MmC3V2FPT0WU
SK-OV-3 NYP3NXBvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2T0S2lEPTB;ND60OlA1OiEQvF2= NEHFWHRUSU6JRWK=
A172 MmXvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGTCSXhKSzVyPUSuOVU2OjFizszN MXjTRW5ITVJ?
NB10 M2LMcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmOxTWM2OD12LkW5O|gyKM7:TR?= Ml:5V2FPT0WU
P30-OHK M{PJOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXLZd|Q2UUN3ME20MlgyQTJ7IN88US=> M13U[HNCVkeHUh?=
KYSE-520 NX3nZ5lFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnriTWM2OD12Lki4NFE5KM7:TR?= MXfTRW5ITVJ?
KNS-62 M1zBTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M33TWWlEPTB;ND65NFgxPyEQvF2= NV3yTnB{W0GQR1XS
ES1 NFywTGdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIXWflBKSzVyPUSuPVYxOzVizszN M3[5dXNCVkeHUh?=
KOSC-2 NIHDUGVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXTQZ5N6UUN3ME20Mlk6QTV7IN88US=> NWDHdYlZW0GQR1XS
TE-9 NIP1XopIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF\FeVVKSzVyPUWuNFE2OjVizszN MVnTRW5ITVJ?
NCI-H1838 M1\XTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnnVTWM2OD13LkCyNFc3KM7:TR?= NWj5cGs5W0GQR1XS
KP-N-YN MoLmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHzvTnRKSzVyPUWuNVI2QTFizszN M3XMOnNCVkeHUh?=
ALL-PO NEjIdINIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1r2[WlEPTB;NT6yNlU5PyEQvF2= MULTRW5ITVJ?
NOMO-1 Ml;nS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH\ZVXdKSzVyPUWuNlMyPzZizszN M1nKN3NCVkeHUh?=
CGTH-W-1 NV2xT3FDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn\jTWM2OD13LkO4OVg1KM7:TR?= MVLTRW5ITVJ?
ATN-1 NYK0[JN1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M37ufmlEPTB;NT60NVE5PCEQvF2= NWftPHlMW0GQR1XS
AGS MlPnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIjWdWtKSzVyPUWuOFk3OThizszN NUPpbHJkW0GQR1XS
SW13 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYTJR|UxRTVwNUOyOlUh|ryP M4nKWHNCVkeHUh?=
SW1990 NYH1[poxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWfJR|UxRTVwNkKyPFUh|ryP NHfMfVlUSU6JRWK=
BFTC-905 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3jjOWlEPTB;NT62OVg4QCEQvF2= MX3TRW5ITVJ?
RMG-I MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVnzfZdEUUN3ME21MlY2QTR{IN88US=> M{D4RnNCVkeHUh?=
HD-MY-Z M1PkT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1PjbWlEPTB;NT62PFU5OSEQvF2= MnHVV2FPT0WU
HCC1187 NU\JTIN7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIjXc2VKSzVyPUWuO|ExOzJizszN MlnuV2FPT0WU
HCC2998 NVXnZ4ZsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYjNPZhLUUN3ME21MlcyQDd5IN88US=> MWrTRW5ITVJ?
RT-112 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEXYRldKSzVyPUWuPVAzPDVizszN NUPEXItXW0GQR1XS
CAKI-1 MlL1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYGxXY95UUN3ME22MlAxQTh3IN88US=> NUTkOHN4W0GQR1XS
HDLM-2 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVX3NGxsUUN3ME22MlAyPDh7IN88US=> M1jZd3NCVkeHUh?=
TE-10 MknKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkXFTWM2OD14LkC0PFM{KM7:TR?= MnnsV2FPT0WU
KE-37 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1rHWmlEPTB;Nj6wO|I2QCEQvF2= MnPtV2FPT0WU
RCM-1 MkXQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH;MWJlKSzVyPU[uNFc4OTlizszN M{DzOHNCVkeHUh?=
Mewo MlLpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn3lTWM2OD14LkGxOFg4KM7:TR?= MlvzV2FPT0WU
L-428 NFf0V45Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYnqfWZzUUN3ME22MlE5ODN5IN88US=> MWrTRW5ITVJ?
8-MG-BA NWWyTI5PT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGjFXnNKSzVyPU[uNVk{PzlizszN NU\MUGNyW0GQR1XS
SK-MEL-28 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3vIWWlEPTB;Nj6yNFk3PyEQvF2= NYXrOIJ2W0GQR1XS
VA-ES-BJ MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWDiclJsUUN3ME22MlMxPDZ{IN88US=> MmrOV2FPT0WU
CAL-120 M3nuU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWXJR|UxRTZwNEWxOlIh|ryP Mon4V2FPT0WU
GAMG MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlHXTWM2OD14LkWwOlIh|ryP M4nL[XNCVkeHUh?=
MSTO-221H MlTES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2PadWlEPTB;Nj61NVE4KM7:TR?= M2LOR3NCVkeHUh?=
GCT MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV\JR|UxRTZwNUOzPFch|ryP NWS0[GFkW0GQR1XS
SH-4 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1LEPGlEPTB;Nj61PFg1PCEQvF2= NWXtb3VpW0GQR1XS
Calu-3 NXXkRldpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M17ScWlEPTB;Nj62OVU3QCEQvF2= NV\MOpVuW0GQR1XS
SK-HEP-1 NIX4WWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX3JR|UxRTZwN{G3OVMh|ryP M{jvOnNCVkeHUh?=
PFSK-1 M123V2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVLJR|UxRTZwN{O0O|gh|ryP M1[0d3NCVkeHUh?=
NB12 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml71TWM2OD14Lke4OFA1KM7:TR?= NEjj[lRUSU6JRWK=
A388 MorJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFHpT2xKSzVyPU[uPFk2OiEQvF2= NFq4T5BUSU6JRWK=
KLE NUWxW4xOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mlj3TWM2OD14Lkm2OVE3KM7:TR?= MWrTRW5ITVJ?
TE-11 NV;DR4h1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVTJR|UxRTdwMES5Olch|ryP MnfJV2FPT0WU
NCI-H2405 MnHyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mlu0TWM2OD15LkC4N|k{KM7:TR?= NXzIZ|ZHW0GQR1XS
OVCAR-4 M2jENWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEDx[YhKSzVyPUeuNVU{PSEQvF2= M3j3V3NCVkeHUh?=
KP-N-YS M{f0Xmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXXJR|UxRTdwMUixOEDPxE1? MXnTRW5ITVJ?
NOS-1 NYDTSYR7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NELtUYhKSzVyPUeuNlU5OjhizszN NFvCUFlUSU6JRWK=
DMS-114 NXvsS2ZyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYLnfo51UUN3ME23MlI6PjNzIN88US=> Mo\CV2FPT0WU
NCI-H2030 NVO1N4tTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4XQ[GlEPTB;Nz6zN|Y6QCEQvF2= MUnTRW5ITVJ?
HEL NIjWUYpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYPJR|UxRTdwM{e1NlEh|ryP M3m4ZnNCVkeHUh?=
CAL-62 NHixflBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYLJR|UxRTdwM{m4OlYh|ryP MmLzV2FPT0WU
COLO-668 MnTLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHuyb|hKSzVyPUeuOFM6PjdizszN NIHhXGlUSU6JRWK=
SKG-IIIa MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MULJR|UxRTdwNEWzPFEh|ryP MoTXV2FPT0WU
HCC1419 MoTRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYTJR|UxRTdwNE[4N|Mh|ryP M2PSR3NCVkeHUh?=
OS-RC-2 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkHOTWM2OD15LkS5PFk1KM7:TR?= NXfjTGN2W0GQR1XS
COLO-829 MknBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEPlbFRKSzVyPUeuOlY1PjNizszN MkO3V2FPT0WU
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IGR-1 NUPlW3F3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnvvTWM2OD1|Nj63NFc2KM7:TR?= M3fX[XNCVkeHUh?=
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ONS-76 MnfSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXKyUIsyUUN3ME2zO{4yOzB7IN88US=> M4XlS3NCVkeHUh?=
T98G NITHTGVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUTJR|UxRTN5Lki2N|Uh|ryP NFe5eYFUSU6JRWK=
NB14 MlHOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlLaTWM2OD1|OD60OVQ1KM7:TR?= NF;xPYxUSU6JRWK=
D-423MG MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmTwTWM2OD1|OD60PFY6KM7:TR?= MnvxV2FPT0WU
NCI-H510A NYLIOGlmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHi5R3RKSzVyPUO4MlY1PTFizszN MmXhV2FPT0WU
NMC-G1 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NY\rXHVnUUN3ME2zPE44OjJ{IN88US=> NYLCbWVVW0GQR1XS
SW948 M4LLZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIHROnZKSzVyPUO4Mlg6PDlizszN NX6zZ2R4W0GQR1XS
MFM-223 Mo\zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYDJR|UxRTN6Lkm0N|Eh|ryP MojoV2FPT0WU
PAN1 M3jld2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUfJR|UxRTN6Lkm5NVkh|ryP M1HLSXNCVkeHUh?=
MZ7-mel NGXhe5hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXPV[XE{UUN3ME2zPU4yOTl7IN88US=> MkXxV2FPT0WU
COLO-679 NHntZ5VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmHPTWM2OD1|OT65O|A5KM7:TR?= MVzTRW5ITVJ?
SW872 NI\DdnRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnrETWM2OD12MD6yNFMyKM7:TR?= M3LycHNCVkeHUh?=
HT-1197 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVnJR|UxRTRyLkK4Olkh|ryP NGWw[JpUSU6JRWK=
ES6 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4nxd2lEPTB;NECuOFU2OiEQvF2= M{OwTHNCVkeHUh?=
SNU-387 MofXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYr2b3NoUUN3ME20NE45OjN6IN88US=> NXHrcFdFW0GQR1XS
T47D MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnLqTWM2OD12MT6wNVkzKM7:TR?= NIHTc3FUSU6JRWK=
G-402 NYLNWllZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYLvcHNqUUN3ME20NU4xQDB7IN88US=> MmXwV2FPT0WU
MZ1-PC MlGwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoDHTWM2OD12MT64NUDPxE1? NH\CNlVUSU6JRWK=
SW1417 MmT4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXTSVGNOUUN3ME20Nk4{ODNizszN NX65N25QW0GQR1XS
RERF-LC-MS NFLve5pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mm\MTWM2OD12Mj61NFg4KM7:TR?= NX7Nb2tJW0GQR1XS
SF539 NGfHdWhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NInMSJFKSzVyPUSyMlU2OjlizszN NVztbFNVW0GQR1XS
ESS-1 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXjZSHJFUUN3ME20N{42PTV6IN88US=> M4Dkd3NCVkeHUh?=
RO82-W-1 MnzOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkKyTWM2OD12Mz62NVU{KM7:TR?= M1v6ZXNCVkeHUh?=
S-117 NFHiRYtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml;YTWM2OD12Mz62PVU5KM7:TR?= M{\6e3NCVkeHUh?=
SW756 NYHNe|VzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVPU[ItZUUN3ME20N{45OjR{IN88US=> NYrlboQ3W0GQR1XS
CP66-MEL NEHIPYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn[zTWM2OD12NT63NVQ{KM7:TR?= NH;qSnlUSU6JRWK=
H-EMC-SS NHLzPXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NU\3ZYprUUN3ME20OU44Pjl|IN88US=> M2LvRXNCVkeHUh?=
NCI-H2052 Ml\zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEjZWIRKSzVyPUS1MlkxOjdizszN NXfGXVVKW0GQR1XS
SW684 NV3IXGFyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYrJR|UxRTR5LkCwPFEh|ryP NFLuZlJUSU6JRWK=
HCC1569 Mn\vS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX7JR|UxRTR5LkG2OVgh|ryP MVTTRW5ITVJ?
KGN NVnRRVhoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{PvR2lEPTB;NEeuN|MyOSEQvF2= MXnTRW5ITVJ?
LCLC-103H M4LrVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEKzcJBKSzVyPUS3MlQ1ODRizszN MoTOV2FPT0WU
SW780 NU\HVpVST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml;1TWM2OD12Nz62N|UyKM7:TR?= M4npU3NCVkeHUh?=
NCI-H1703 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVPJR|UxRTR6LkOzOVIh|ryP NHHlXYdUSU6JRWK=
HCC1395 NUWweIVLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnzYTWM2OD12OD63OFMzKM7:TR?= NX\kTndvW0GQR1XS

... Click to View More Cell Line Experimental Data

In vivo Gefitinib (100 mg/kg) improves the anti-tumor effect of radiotherapy in LoVo tumor xenografts. [3] Gefitinib treatment of nude mice bearing established human GEO colon cancer xenografts reveals a reversible dose-dependent inhibition of tumor growth because GEO tumors resumes the growth rate of controls at the end of the treatment. [4]

Protocol

Cell Research:[1]
+ Expand
  • Cell lines: NR6, NR6M and NR6W cells
  • Concentrations: 0-2 μM
  • Incubation Time: 72 hours
  • Method: Exponentially growing cells including NR6, NR6M, NR6M and NR6W cells are seeded in sextuple in 96-well plates at a concentration of 2000 cells/well, allowed to adhere and subsequently washed in PBS and incubated overnight in medium containing 0.5% FCS. Cells are then treated with varying concentrations (0-2 μM) of Gefitinib or the solute control DMSO and EGF. The optimal EGF concentration for inducing proliferation of NR6wtEGFR and NR6W cells has previously been determined and hence NR6wtEGFR and NR6W cells are supplemented with 10 nM and 0.1 nM EGF, respectively. EGF is not added to NR6 and NR6M cells. After 72 hours the amount of cells are measured by performing a MTT proliferation assay.
    (Only for Reference)
Animal Research:[3]
+ Expand
  • Animal Models: Female nude mice (cba nu/nu) aged 8–10 weeks are intra-dermal injected with LoVo cells.
  • Formulation: 0.5% polysorbate
  • Dosages: 100 mg/kg
  • Administration: Once daily by oral administration (0.1 mL/10 g body weight) for 14 days
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 89 mg/mL (199.14 mM)
Ethanol 4 mg/mL (8.95 mM)
Water Insoluble
In vivo Add solvents individually and in order:
1% CMC Na
30mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 446.90
Formula

C22H24ClFN4O3

CAS No. 184475-35-2
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
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    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03050164 Completed Bioequivalence Study Hunan Kelun Pharmaceutical Co., Ltd. September 8, 2016 Phase 1
NCT02856893 Not yet recruiting NSCLC European Organisation for Research and Treatment of Cancer - EORTC|AstraZeneca March 2017 Phase 2
NCT02976116 Recruiting NSCLC Hutchison Medipharma Limited December 2016 Phase 2
NCT02951637 Not yet recruiting Lung Adenocarcinoma Shanghai Chest Hospital|Ruijin Hospital|Changhai Hospital December 2016 Phase 2
NCT02930954 Not yet recruiting Non-small-cell Lung Cancer Caicun Zhou|Tongji University November 2016 Phase 2
NCT02929693 Enrolling by invitation Cancer Shanghai University of Traditional Chinese Medicine|Shanghai Chest Hospital|Shanghai Pulmonary Hospital, Shanghai, China October 2016 Phase 3

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    I try to dissolve Gefitinib (ZD1839) in 0.5% methylcellulose+0.2% Tween 80 for in vivo experiments. However, when I prepare the mixture, it forms precipitates. Exists another way to dissolve completely the compound?

  • Answer:

    S1025 can be dissovled in both 5% DMSO+corn oil and 5% DMSO+30% PEG 300+5% Tween 80+ddH2O at 2.5 mg/ml clearly. It dissolves in DMSO not readily, please sonicate and warm it in water bath at about 45 degree, and dissolve it clearly first. Then add other solvent.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID