Gefitinib (ZD1839)

Catalog No.S1025

Gefitinib (ZD1839) Chemical Structure

Molecular Weight(MW): 446.90

Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.

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Cited by 91 Publications

12 Customer Reviews

  • Perturbation of EGFR by its ligand EGF and gefitinib (ZD-1839 Iressat; inhibits EGFR) produces opposite responses in the predicted EGFR target genes SOCS2 and NR2E1.

    Mol Syst Biol 2011 7, 486. Gefitinib (ZD1839) purchased from Selleck.

    Effects of shRNA-mediated RECK depletion on EGFR signaling in MEFs. (a) Immunoblotting (IB) of the indicated proteins in wild-type MEFs transduced with the indicated shRNAs and cultured for 48 h in the presence of 0.1% dimethyl sulfoxide (DMSO; vehicle) or 1 mM gefitinib (S1025, Selleck Chemicals,Houston, TX, USA). (b) Immunoprecipitation from whole lysates of cells from panel a with an anti-phosphorylated tyrosine (pTyr) antibody. Precipitates from 500 mg protein in whole cell lysates (upper) or 20 mg protein in whole cell lysates (lower) were analyzed by IB with anti-EGFR antibody.

    Oncogene 2010 30, 737-750. Gefitinib (ZD1839) purchased from Selleck.

  • (B–C) LNCaP (B) and LNCaP-AI (C) cells were transiently transfected with sPLA2-IIa(-800)-Luc (0.5 μg). The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 lM) and Bortezomib (20 μM) without or with EGF (100 ng/ml) for 24 h. Luciferase assay was performed according to a standard protocol with Renilla luciferase as an internal control. Data are presented as the mean (±SD) of duplicate values of a representative experiment that was independently repeated for five times.

    Carcinogenesis 2010 31, 1948–1955 . Gefitinib (ZD1839) purchased from Selleck.

    LNCaP-AI cells were starved in 1% stripped medium for 24 h. The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 μM) and Bortezomib (20 μM) for 24 h. Cell culture medium was collected from each sample and subjected to ELISA for sPLA2-IIa. The condition medium samples were diluted 10 times for ELISA. Average of duplicate samples was converted to nanogram per milliliter against standard curve. The data represent one of five repeated experiments.

     

     

    Carcinogenesis 2010 31, 1948–1955. Gefitinib (ZD1839) purchased from Selleck.

  • Susceptibility of lung cancer cells to cytolytic activity of NK-92 cells after treatment with EGFR inhibitors. Three lung cancer cells were untreated (open circle) or treated with 10 μM erlotinib or gefitinib (black filled square or black filled triangle) for 24 hours. The lung cancer cells were cocultured with NK-92 cells at indicated effector to target ratio (E:T ratio). To determine the specificity of NKG2D-mediated cytolysis of lung cancer cells, NK-92 cells were preincubated with blocking mAb against NKG2D before the assay (gray filled square and triangle) and correspond-ing isotype mAb (open square and triangle).

    J Immunother 2011 34(4), 372-81. Gefitinib (ZD1839) purchased from Selleck.

    Suppression of EGFR signaling by Gefitinib is dose dependent and non- toxic at millimolar concentrations.Results from plaque reduction tests of Hep2 cells infected with VACV(12.5pfu/well) treated with a serial dilution of Gefitinib (1000-0.01 m) and analyzed for (A)proliferation as indicator of cytotoxicity,(B) IC50 of plaque size inhibition, (C)dose dependent inhibition of EGFR-ERK1/2signaling and VACV proteins by Western blot analysis (pEGFR detection was obtained from a different experiment with identicals ettings) and (D)dose dependent inhibition of orthopoxvirus genome replication by real-time PCR.Untreated VACV infectedcells and uninfected cells are shown as controls in the right panel.

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

  • A circular zone of EGFR activated but uninfected cells facilitate virus induced plaque development , efficiently blocked by Gefitinib. (A)Immun of luorescent staining of aplaque reduction tests with Hep2 cells 24,48,72 and 96 h after VACV infection (6.25pfu/chamber). VACV infected cells(green) were visualized by human IgG directed to orthopoxviruses (VIG) detected with a secondary FITC labelled antibody. Uninfected cells with activated EGFR signaling(red) are detected with a secondary Cy3labelled antibody. Positive cells for both , VACV and phosphorylated EGFR are shown in yellow(merge). Cellular nuclei are counterstained with DAPI. (B)Immunofluorescent staining of a plaque reduction test as described for (A) and treated with three different Gefitinib concentrations (1000 nM, 1 nM, and 0.01 nM) . The test was analyzed 96h afterVACV infection.Uninfected cells are shown as controls. Magnification100×.(For interpretation of the references to color in this figure legend , the reader is referred to the web version of the article.)

     

     

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

    The EGF-dependent increased association of PCM1 and AZI1 requires EGFR kinase activity. ZD1839 (Iressa)—EGFR tyrosine kinase inhibitor.

    Mol Biosyst 2011 7, 3223-33. Gefitinib (ZD1839) purchased from Selleck.

  • Reduction of GPER expression in HCC1806 cells after treatment with gefitinib. (A) Concentration dependence. HCC1806 cells were treated with 50-500 nM gefitinib for 48 h. GPER was detected by western blotting. (B) Time dependence. HCC1806 cells were treated with 200 nM gefitinib for 24-96 h. ap<0.05 vs. control; bp<0.001 vs. control. Mean values and SE of three independent experiments are shown. (C) Representative western blotting of concentration dependence. (D) Representative western blotting of time dependence

    Oncol Rep, 2017, 37(2):1212-1218. Gefitinib (ZD1839) purchased from Selleck.

    Inhibition of signaling pathway activation in lung tumor cell lines by kinase inhibitors. Lung tumor cells were cultured in 10% FBS until reaching ∼80% confluence and then the cells were starved in serum-free medium for overnight, followed by 4-hour treatment with the inhibitors. Cell lysates were then prepared and used for determination of the pathway activation signals by the CEER assay.

    Int J Proteomics 2011 215496. Gefitinib (ZD1839) purchased from Selleck.

  • Breast cancer cells were pretreated with 100ng/ml EGF for 15 min and then treated with the indicated concentrations of  Gefitinib for 24 hours.

     

     

    2010 Dr. Zhang of Tianjin Medical University. Gefitinib (ZD1839) purchased from Selleck.

    MTT assay result. 0-500nM Gefitinib treated with H1299, H358, H25 and HCC827 cell lines by 72h, Proliferation rate was tested.

     

     

    2010 Dr. Vicky Tin from University of Hong Kong. Gefitinib (ZD1839) purchased from Selleck.

Purity & Quality Control

Choose Selective EGFR Inhibitors

Biological Activity

Description Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.
Features A potent EGFR tyrosine kinase inhibitor.
Targets
Tyr1173 (NR6W cells) [1] Tyr1173 (NR6wtEGFR cells) [1] Tyr992 (NR6wtEGFR cells) [1] Tyr992 (NR6W cells) [1]
26 nM 37 nM 37 nM 57 nM
In vitro

Gefitinib effectively inhibits all tyrosine phosphorylation sites on EGFR in both the high and low-EGFR-expressing cell lines including NR6, NR6M and NR6W cell lines. The phosphorylation sites Tyr1173 and Tyr992 are less sensitive requiring higher concentrations of Gefitinib for inhibition. Gefitinib effectively blocks the phosphorylation of PLC-γ, with IC50 of 27nM, in NR6W cells. The NR6wtEGFR and NR6M cell lines has low levels of PLC-γ phosphorylation but the level in the NR6M cell line is more resistant to inhibition by Gefitinib with IC50 of 43 nM and 369 nM, respectively. Gefitinib inhibits Akt phosphorylations, with IC50 of 220 and 263nM, in the low-EGFR- and -EGFRvIII-expressing cell lines, respectively. Gefitinib in the dose range from 0.1 to 0.5μM significantly facilitates, rather than abrogates, colony formation of NR6M cells. However, at a concentration of 2 μM Gefitinib completely blocks NR6M colony formation. Gefitinib rapidly and in a dose-dependent manner inhibits EGFR and ERK phosphorylation up to 72 hours after EGF stimulation in both the high- and low-EGFR-expressing cell lines. [1] Gefitinib is the monolayer growth of these EGF-driven untransformed MCF10A cells with an IC50 of 20 nM. [2] The combination of Gefitinib (0.2 μM and 0.5 μM) with irradiation lead to a significant growth inhibition in LoVo cells, compared with radiation alone.. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KB NYPFPWN7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1fCUGlEPTB;MD6wOVQh|ryP MWOxOlUyPjR5Mx?=
NIH3T3 NFXB[nJHfW6ldHnvckBCe3OjeR?= MmfyNE4zKM7:TR?= NILSPJAzKGh? NF;p[JpFVVOR M{L6XGlvcGmkaYTpc44hd2ZiRVfGJJN1cW23bHH0[YQhcHWvYX6g[ZJjSjFiYYX0c5Bpd3OyaH;yfYxifGmxbjD3bZRpKEmFNUCgc4YhOC5yMUS0JO69VQ>? MVmxOlQ5ODJ6NB?=
COS7 MlfvSpVv[3Srb36gRZN{[Xl? NX7zO2ZOQTZizszN MX\Jcohq[mm2aX;uJI9nKGi3bXHuJGpCUzJiVk[xO2YhdXW2YX70JIV5eHKnc4Pl[C=> NHTzXFkyPzF5OEeyNi=>
A549 NWG4N5FjS3m2b4TvfIlkKEG|c3H5 NUXw[HBCQTZiaB?= NHnaOYtFVVOR NIf4ZohKSzVyPUGzMlU6KM7:TR?= NX36ZYZHOTh|MUO4NFc>
K562 MkPOR5l1d3SxeHnjJGF{e2G7 M{[1R|k3KGh? NV36dG0zTE2VTx?= NWDQTW5iUUN3ME25MlM3KM7:TR?= MknENVg{OTN6MEe=
MDA-MB-231 NH7pZ|NEgXSxdH;4bYMhSXO|YYm= MlO0PVYhcA>? M4e5ZWROW09? NIT0WoxKSzVyPUO2MlYyKM7:TR?= NXXuVJFXOTh|MUO4NFc>
MOLT4 NVn4NYxkS3m2b4TvfIlkKEG|c3H5 NYDmNGlYQTZiaB?= M2rD[2ROW09? MoH5TWM2OD1zNT6wNkDPxE1? NEXYOYwyQDNzM{iwOy=>
MCF7 NILQTWhEgXSxdH;4bYMhSXO|YYm= NHvyPFg2OCEQvF2= NG\OcHA4OiCq MmDNTWM2OD1zMj6wOUDPxE1? NUnTWmluOTh5N{G4NVk>
BT474 Mki5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3;nUlczKGh? MmD6RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCEVES3OEBk\WyuczDveoVz\XiycnXzd4lv\yCHUlLiNkB4cXSqIFXDOVAhd2ZiMD6zJO69VQ>? M1S5e|E6ODJ6NEK0
HN5 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGH5Uos4OiCq M3\oTWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSF61JINmdGy|IH;2[ZJmgHC{ZYPzbY5oKEWJRmKge4l1cCCHQ{WwJI9nKDBwMEGg{txO M3XBWFE6ODJ6NEK0
BA/F3 M1PScmN6fG:2b4jpZ{BCe3OjeR?= Mnv3O|IhcA>? M2XKPGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJGJCN0Z|IHPlcIx{KGW6cILld5NqdmdiRVfGVkBNQDV6UjDteZRidnRid3n0bEBKSzVyIH;mJFAvODFyODFOwG0> NVPMS2ZjOTl{M{myNlk>
NIH3T3 NULWZXpQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MojkO|IhcA>? NH3qNpRIem:5dHigbY5pcWKrdHnvckBw\iCvb4Xz[UBPUUhxM2SzJINmdGy|IHX4dJJme3Orbneg[ZJDOiCpZX7lJJdqfGhiSVO1NEBw\iByLkW0JO69VQ>? NIrvOGsyQTZ4NUO3Oy=>
MCF7 MX M1rnW2Z2dmO2aX;uJGF{e2G7 NXXodVZnOzBibXnu MWrJcohq[mm2aX;uJI9nKEKFUmCg[ZhxemW|c3XkJJdqfGhiSVO1NEBw\iBzLkGg{txO M2O4XVE6QTN{OU[w
A431 NUW5W3FZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{jNUFExKM7:TR?= NF;jZZY4OiCq M{T0dmdKPTB;MD61NVQh|ryP MlGwNVk6Pjl2NkW=
A431 M1T0b2tqdmG|ZTDBd5NigQ>? NXfSOGJJOC5zIN88US=> NGmxN2IyKGh? MXXJcohq[mm2aX;uJI9nKEWJRj3pcoR2[2WmIFXHSnIheGixc4Doc5J6dGG2aX;u Mk\VNVk6Pjl2NkW=
A431 MYHLbY5ie2ViQYPzZZk> NXfTXWhDOTByIN88US=> Ml3VNVAhdWmw NX\IWWRvWHKxbH;u[4VlKGmwaHnibZRwenliYXP0bZZqfHlib3[gSWdHNWmwZIXj[YQhTUeIUjDwbI9{eGixconsZZRqd25? NF3uPJoyQTl4OUS2OS=>
Sf9 MkW3T4lv[XOnIFHzd4F6 M{Pod|ExKG2rbh?= M4nlTWROW09? MXPJcohq[mm2aX;uJI9nKEWJRmKg[ZhxemW|c3XkJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gdoVk\XC2b4KgZZV1d3Cqb4PwbI9zgWyjdHnvckB4cXSqIFnDOVAhd2ZiMD6wN|Mh|ryP Mn3INlAxPTZ2MkW=
NCI-H1975 Mk\5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MU[1JO69VQ>? MmrHO|IhcA>? NHjOcYxKSzVyPUiuNlYh|ryP M4jTeFIxOTVzNkew
NCI-H1975 MYLLbY5ie2ViQYPzZZk> MVuxNEDPxE1? NHjiSGJKdmirYnn0bY9vKG:oIFXHSnIh[XW2b4Doc5NxcG:{eXzheIlwdg>? MnnJNlAyPTF4N{C=
NCI-H1975 M{HHOmZ2dmO2aX;uJGF{e2G7 MV[xNEDPxE1? NXL1dnFpUW6qaXLpeIlwdiCxZjDFVmJDOiCjdYTvdIhwe3Cqb4L5cIF1cW:w NYDkZWpyOjBzNUG2O|A>
NCI-H1975 NFTnd5VHfW6ldHnvckBCe3OjeR?= NWD6em1SPSEQvF2= NHj5bG81QCCq NHLFUZBKdmS3Y4Tpc44hd2ZiYYDvdJRwe2m|IHHzd4V{e2WmIHHzJIFkfGm4YYTpc44hd2ZiY3HzdIF{\S1| M3fBR|IxOTVzNkew
A375 NEPvWYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEG1cWM1QCCq M{fQfGlEPTB;MkCuOVUh|ryP MV:yNFMxPDV|Nx?=
SMMC7721 M4fGTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUPZd|h4PDhiaB?= MUTJR|UxRTN|LkGyJO69VQ>? MmHtNlA{ODR3M{e=
DU145 NFHpOXFEgXSxdH;4bYMhSXO|YYm= M2LsUlUh\A>? NYjzXphbTE2VTx?= MXzJR|UxRTR{LkG3JO69VQ>? M2TNSFIxPDZ4NUW1
MIAPaCa2 MVnDfZRwfG:6aXOgRZN{[Xl? MUO1JIQ> NV;FbJF7TE2VTx?= MlHFTWM2OD13MD62NkDPxE1? NIrke28zODR4NkW1OS=>
PANC1 MknhR5l1d3SxeHnjJGF{e2G7 M1\yZ|Uh\A>? M2njT2ROW09? M3:0[GlEPTB;NECuOFIh|ryP MX6yNFQ3PjV3NR?=
PC9 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlHpRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDFS2ZTNWSnZnnjbYVvfCCqdX3hckBRSzliY3XscJMhf2m2aDDFR|UxKG:oIECuNFE4KM7:TR?= NVuzV5doOjF{MEi4NFI>
PC9 NXzVS45pT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHfyc5d1cC2{ZYPpd5RidnRiaIXtZY4hWEN7IHPlcIx{KGW6cILld5NqdmdiRVfGVkBGPzR4X1G3OVAwXDd7MF2gcZV1[W62IIfpeIghTUN3MDDv[kA4KM7:TR?= MlzhNlEzODh6MEK=
PC3 NVjlT2E6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWexNEDPxE1? NXT5ZZVbPzJiaB?= MVjEUXNQ NHLSdopKSzVyPUeuOEDPxE1? MomwNlE{PTN3NE[=
MDA-MB-468 NVrkUWQyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml73NVAxKM7:TR?= MVy0PEBp M{\RNGROW09? M1fNcWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5 MoHINlE1Ojl4M{K=
Plasmodium falciparum MU\xTHRUKHC{b3\pcIlv\yCob4KgbY5pcWKrdH;yd{Bw\iCSbHHzcY9lcXWvIH\hcINqeGG{dX2gdJJwdGmoZYLheIlwdg>? M4SyPVIyQDF5MES1
NCI-H1993 M3q4WGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mnf2O|IhcA>? MnrGSG1UVw>? M4\SN2lEPTB;MD6xJO69VQ>? MnrBNlE6OTZ2M{O=
NCI-H292 M4\YVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmnJO|IhcA>? NYrtcnQzTE2VTx?= NU\zXVh1UUN3ME2wMlAzKM7:TR?= M1\IblIyQTF4NEOz
A431 MV7LbY5ie2ViQYPzZZk> MWmyJIg> MWHEUXNQ MX;Jcohq[mm2aX;uJI9nKEWJRmKgdIhwe3Cqb4L5cIF1cW:wIHnuJGVITi2|dHnteYxifGWmIHj1cYFvKEF2M{Gge4l1cCCLQ{WwJI9nKDBwMECxJO69VQ>? MX2yNlExOTF|Mh?=
MCF7 M{\oU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3nseFExOMLizsznM41N MXO0PEBp MVTEUXNQ M1jLO2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDveoVz\XiycnXzd4lv\yCHR1\SJIdmdmVid3n0bEBKSzVyIH;mJFEzNjB3IN88US=> NF;xdm4zOjFzOUGzNC=>
BT474 M4PzU2N6fG:2b4jpZ{BCe3OjeR?= MVWxNEDPxE1? M1rsUVQ5KGh? MY\DfZRwfG:6aXPpeJkh[WejaX7zeEB{\XK3bT3zeIFzfmWmIHj1cYFvKEKWNEe0JINmdGy| NYTlPYZbOjJzM{izNFg>
MCF7 NV\QfItkS3m2b4TvfIlkKEG|c3H5 MYWxNEDPxE1? NGjvSG81QCCq M3LpfmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KHOncoXtMZN1[XK4ZXSgbJVu[W5iTVPGO{Bk\WyuczDveoVzNWW6cILld5NqdmdiRYLiRlIh[2WubIO= MkfaNlIyOzh|MEi=
HCC827 NWrSOGtRU2mwYYPlJGF{e2G7 NU\BcZhCOC5zMkWg{txO NE\yS|EzPCCq MnvFSG1UVw>? MVvJcohq[mm2aX;uJI9nKEWJRmKg[IVtKEV5NE[tRVc2OCCvdYThcpQhYTFyNkigZZV1d3Cqb4PwbI9zgWyjdHnvci=> NXLHdodSOjJ|M{mzOFI>
HCC827 M2fGXGtqdmG|ZTDBd5NigQ>? MmK1NE4yOjVizszN NX7SOYZoOjRiaB?= NFLSWZBFVVOR MmXJTY5pcWKrdHnvckBw\iCHR1\SJIRmdCCHN{S2MWE4PTBibYX0ZY51NW2nZHnheIVlKEW{azDwbI9{eGixconsZZRqd25? NV\j[JJrOjJ|M{mzOFI>
HCC827 NUW5cIF2U2mwYYPlJGF{e2G7 NH3sS28xNjF{NTFOwG0> M{LCO|I1KGh? NHW2O5FFVVOR NV\HTVVVUW6qaXLpeIlwdiCxZjDFS2ZTKGSnbDDFO|Q3NUF5NUCgcZV1[W62LX3l[IlifGWmIFHreEBxcG:|cHjvdplt[XSrb36= MVeyNlM{QTN2Mh?=
A431 NFjuempMcW6jc3WgRZN{[Xl? Mor1NVAxKM7:TR?= MVmzNEBucW5? NILpfVlKdmirYnn0bY9vKG:oIFXHSnIh\GW{aY\l[EBnem:vIHj1cYFvKEF2M{GgZ4VtdCCvZX3idoFv\SCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKHCxbImtS4x2NVS7cjDwbI9{eGixconsZZRqd25id3n0bEBKSzVyIH;mJFEvODhizszN MXOyNlY6QDd6Mh?=
H460 Mnm0R5l1d3SxeHnjJGF{e2G7 M4r1dVczKGh? NXKw[nJ6UUN3ME21MlU6KM7:TR?= MYKyNlczPzR2OR?=
HT-29 NHnHOpBEgXSxdH;4bYMhSXO|YYm= MoTUO|IhcA>? M17peGlEPTB;Mz6zOkDPxE1? MWmyNlczPzR2OR?=
SGC7901 NEPIU5hEgXSxdH;4bYMhSXO|YYm= M1\DOVczKGh? M3PNVGlEPTB;MUCuNlYh|ryP NVf1RpN7OjJ5Mke0OFk>
A498 NXXofld[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn7lRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCDNEm4JINmdGy|IHX4dJJme3OrbnegSWdHWiCjbnSgV3JEKHerdHigTWM2OCCxZjC4MlYh|ryP NESzVHYzOjhzOEi0PC=>
Calu3 MUTDfZRwfG:6aXOgRZN{[Xl? M3rhSlExKM7:Zz;tUC=> MmTFO|IhcA>? M37HXWlEPTB;MT62JO69VQ>? M3[yVlI{OTF4MU[4
FADU M2jLU2N6fG:2b4jpZ{BCe3OjeR?= NW\OOpRyOTBizsznM41N NEnkc|U4OiCq M1HVWmlEPTB;MD6wOUDPxE1? MUGyN|EyPjF4OB?=
HCC827 M136d2N6fG:2b4jpZ{BCe3OjeR?= M2TtVFExKM7:Zz;tUC=> MVW3NkBp MlTNTWM2OD1yLkCwNVUh|ryP M{XibFI{OTF4MU[4
PC9 NHHDUndIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWrSbY91OTBizsznM41N NVjpV|V3PzJiaB?= NUDrOJE{UUN3ME2wMlAxOTRizszN NF;uXoUzOzFzNkG2PC=>
Sf9 NXrqZVRXU2mwYYPlJGF{e2G7 MlXZNUBp NH\0OmFFVVOR MX7Jcohq[mm2aX;uJI9nKGi3bXHuJGdUXC22YXfn[YQhTUeIUjDMPFM1WiCvdYThcpQh\XiycnXzd4VlKHerdHigTWM2OCCxZjCwMlAyPSEQvF2= NWq2N21UOjN4MUG2PVE>
16HBE14o- NWfISlZuS3m2b4TvfIlkKEG|c3H5 MX[xNEDPxE1? NXf2enNpPzJiaB?= NWrvdHhqTE2VTx?= M{G0R2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJFE3UEKHMUTvMUBk\WyuczDoZZJjd3Krbnege4lt\CC2eYDlJGVITlJiYYPz[ZN{\WRiYYOg[5Jwf3SqIHnubIljcXSrb36ge4l1cCCLQ{WwJI9nKDF{LkexJO69VQ>? MXiyN|Y3QDR2MR?=
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NCI-H2170 NGTXU5BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVjJR|UxRTJwM{S3PFMh|ryP NF[5UmVUSU6JRWK=
EW-18 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4\ZTGlEPTB;Mj6zOVQ4OSEQvF2= MljHV2FPT0WU
KU812 MnvXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV3JR|UxRTJwM{[3O|kh|ryP MWfTRW5ITVJ?
NY MnrvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXHJR|UxRTJwM{eyO{DPxE1? MmXTV2FPT0WU
HTC-C3 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEPlfVJKSzVyPUKuOFIzPjdizszN Mki0V2FPT0WU
CFPAC-1 NYDVc3lmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWK4NFBpUUN3ME2yMlQ{QDZ2IN88US=> MkDmV2FPT0WU
HH MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2\xTWlEPTB;Mj60PVg2PCEQvF2= M3PZfnNCVkeHUh?=
MKN7 M13XXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXPGOYNzUUN3ME2yMlYzPzR6IN88US=> NF7NXGlUSU6JRWK=
TE-8 M32wZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHTISo1KSzVyPUKuOlM3PTNizszN NXPFeI86W0GQR1XS
MC-IXC M2L5Smdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1;tRmlEPTB;Mj62OFQ2OyEQvF2= NUj3W2dIW0GQR1XS
DMS-79 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUDlVnZ{UUN3ME2yMlY6Ojh2IN88US=> M4jMd3NCVkeHUh?=
Detroit562 MkLJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mm\OTWM2OD1{Lk[5PFQ2KM7:TR?= MoDaV2FPT0WU
HuP-T4 MlnOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn;1TWM2OD1{LkeyPFE5KM7:TR?= NF3vRZVUSU6JRWK=
QIMR-WIL MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXL0PJNXUUN3ME2yMlc2QTV{IN88US=> MljkV2FPT0WU
BB65-RCC M1vIVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX\JR|UxRTJwOEC3NVMh|ryP NGixdHRUSU6JRWK=
PC-3 NVPsS2NrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mkm4TWM2OD1{LkizN|E4KM7:TR?= MWHTRW5ITVJ?
OVCAR-3 NWT5bpVXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4DH[GlEPTB;Mj64OVU6PCEQvF2= MkWxV2FPT0WU
KYSE-510 Mlm5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFXHS5NKSzVyPUKuPFY4QDZizszN NV3DSll7W0GQR1XS
HC-1 NUPOTIh4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M33kSmlEPTB;Mj65OFAyOiEQvF2= MUDTRW5ITVJ?
NCI-H1693 M{jGWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXX6RYl2UUN3ME2yMlk2QDl5IN88US=> M1GyUHNCVkeHUh?=
DU-4475 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3TqcWlEPTB;Mj65PVA2OiEQvF2= Mn[1V2FPT0WU
COLO-680N NEDpUWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmfyTWM2OD1|LkCwOFk2KM7:TR?= M3;EeHNCVkeHUh?=
MN-60 NHT1UZJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4\TdmlEPTB;Mz6wOFk4PCEQvF2= MVXTRW5ITVJ?
BCPAP Moi5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX;oOGtqUUN3ME2zMlA5Ojl3IN88US=> MkX2V2FPT0WU
647-V MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWrjblVFUUN3ME2zMlE3OjJizszN MkHyV2FPT0WU
HT-1376 M1HEdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2rOXWlEPTB;Mz6yNFUxPiEQvF2= MWTTRW5ITVJ?
NCI-H1793 MlvmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoKzTWM2OD1|LkKxNFk5KM7:TR?= NWixO5pTW0GQR1XS
SW1463 NXnGWmlwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NViwWnpSUUN3ME2zMlI3OTl{IN88US=> MnfUV2FPT0WU
NCI-H727 NIjTfYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2P4VmlEPTB;Mz6zPFU5PCEQvF2= M{fkbHNCVkeHUh?=
LB771-HNC MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NG\6d2tKSzVyPUOuN|kyOjZizszN NGnSRlZUSU6JRWK=
D-542MG NUHVUnJXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYDJR|UxRTNwNESyNlIh|ryP M1rSd3NCVkeHUh?=
NTERA-S-cl-D1 NHfCfZVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2\qRmlEPTB;Mz60OFk2PyEQvF2= NGK0XYFUSU6JRWK=
C8166 NEnpN|JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVLJR|UxRTNwNE[1NlEh|ryP M{nyZXNCVkeHUh?=
639-V NXTCW|dnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoLCTWM2OD1|LkW3O|kzKM7:TR?= M4XJVXNCVkeHUh?=
TE-1 MlT0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIHXZXJKSzVyPUOuOVkyPjlizszN NIP3XWxUSU6JRWK=
OAW-28 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWG4dZpJUUN3ME2zMlU6PDJ4IN88US=> NV3ucolWW0GQR1XS
SK-LU-1 NGDDZldIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3LGS2lEPTB;Mz62NFY1PSEQvF2= MUfTRW5ITVJ?
CaR-1 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHHGdI1KSzVyPUOuOlExPDhizszN MVnTRW5ITVJ?
A2780 M3foWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml[0TWM2OD1|Lk[0NFg6KM7:TR?= NGDieIxUSU6JRWK=
C2BBe1 NGDm[5VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3Hx[WlEPTB;Mz62OFQxPyEQvF2= MYnTRW5ITVJ?
SK-MES-1 M3zWPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHf2VYNKSzVyPUOuOlQ3OTRizszN M2\mbHNCVkeHUh?=
PANC-10-05 NHHPcJBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXXWfYl5UUN3ME2zMlczODd{IN88US=> MlLTV2FPT0WU
J-RT3-T3-5 NIWxXlVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEDKWpBKSzVyPUOuO|U1ODZizszN MUTTRW5ITVJ?
ES4 NI\oR|NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVLTPVVnUUN3ME2zMlgzOjh4IN88US=> M3W5PHNCVkeHUh?=
NCI-H28 NVnPUFlsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXTJR|UxRTNwOEK5OlEh|ryP NFfuNHVUSU6JRWK=
NCI-H1155 NVnz[JR1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MojjTWM2OD1|Lki0NVU6KM7:TR?= MUTTRW5ITVJ?
KURAMOCHI MnXES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3nSb2lEPTB;Mz64OlA2PiEQvF2= MnfvV2FPT0WU
NCI-H69 NUXvRodjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoDqTWM2OD1|LkmwNVc5KM7:TR?= NIjodWJUSU6JRWK=
IST-MES1 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWfpRY1GUUN3ME2zMlk1ODJ7IN88US=> MmXwV2FPT0WU
NH-12 M2H4[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1rQU2lEPTB;Mz65OFE5PyEQvF2= NHXCeWtUSU6JRWK=
JVM-2 M2DKdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYjJR|UxRTNwOUS3OVIh|ryP NGHwWGRUSU6JRWK=
EW-16 NIf0XXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkXqTWM2OD1|Lkm1OVc4KM7:TR?= NFHrb3JUSU6JRWK=
NCI-H441 NHzFU|lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1jtPWlEPTB;Mz65O|AyPCEQvF2= NXrSWVVNW0GQR1XS
RCC10RGB NUDG[YpTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYrmTXF[UUN3ME20MlAyOjR4IN88US=> Ml3HV2FPT0WU
TCCSUP M4LIc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn\rTWM2OD12LkGxNFUh|ryP MnfrV2FPT0WU
HL-60 M1HkSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2TlNWlEPTB;ND6xNlI1OSEQvF2= MnjOV2FPT0WU
EW-11 NUf3ZYZzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUXJR|UxRTRwMUOyOlEh|ryP MVzTRW5ITVJ?
KARPAS-299 NVvQcVhKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3TuOWlEPTB;ND6zNVAyOyEQvF2= M4jUc3NCVkeHUh?=
SNU-C2B NFP5XmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmHZTWM2OD12LkO0Olg3KM7:TR?= MVPTRW5ITVJ?
LU-139 NYewSWxOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVmzc3dTUUN3ME20MlM4OjR5IN88US=> NIP4fFNUSU6JRWK=
HCC1937 MkTrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmWwTWM2OD12LkO3PVk{KM7:TR?= NFrqZXVUSU6JRWK=
GR-ST Mn7PS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4LFUWlEPTB;ND60N|E5KM7:TR?= Mm\EV2FPT0WU
SK-OV-3 MmLjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYjJR|UxRTRwNE[wOFIh|ryP NWrq[29EW0GQR1XS
A172 NYPXVVJqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHnsNlVKSzVyPUSuOVU2OjFizszN MWrTRW5ITVJ?
NB10 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmPkTWM2OD12LkW5O|gyKM7:TR?= NWCwU|kyW0GQR1XS
P30-OHK NYLWUGx3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmXOTWM2OD12LkixPVI6KM7:TR?= NV:5dYtSW0GQR1XS
KYSE-520 MojYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXHJR|UxRTRwOEiwNVgh|ryP NXPFN3l1W0GQR1XS
KNS-62 NFK2e2FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVL6Nnc2UUN3ME20MlkxQDB5IN88US=> NEjnWHlUSU6JRWK=
ES1 M3TGdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlzaTWM2OD12Lkm2NFM2KM7:TR?= MX3TRW5ITVJ?
KOSC-2 Mlj2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYriOGY6UUN3ME20Mlk6QTV7IN88US=> NHu4elJUSU6JRWK=
TE-9 MmfIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnfwTWM2OD13LkCxOVI2KM7:TR?= MVnTRW5ITVJ?
NCI-H1838 Ml3qS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M37wTGlEPTB;NT6wNlA4PiEQvF2= MV7TRW5ITVJ?
KP-N-YN MorBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWDoc5FbUUN3ME21MlEzPTlzIN88US=> M3TsWnNCVkeHUh?=
ALL-PO MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MULJR|UxRTVwMkK1PFch|ryP MWnTRW5ITVJ?
NOMO-1 NUTXbGtOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NU\5WZRUUUN3ME21MlI{OTd4IN88US=> NXz0OI9SW0GQR1XS
CGTH-W-1 NYDJfIY{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1XFSWlEPTB;NT6zPFU5PCEQvF2= MojDV2FPT0WU
ATN-1 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{j4T2lEPTB;NT60NVE5PCEQvF2= MWHTRW5ITVJ?
AGS NHXZ[I1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEPYZ4hKSzVyPUWuOFk3OThizszN NFfTVnBUSU6JRWK=
SW13 NHHPZlZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2OyeGlEPTB;NT61N|I3PSEQvF2= Mn;XV2FPT0WU
SW1990 MkTvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NW[0VJhHUUN3ME21MlYzOjh3IN88US=> M1OyfnNCVkeHUh?=
BFTC-905 NFnOOmtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYLJR|UxRTVwNkW4O|gh|ryP NGXySJJUSU6JRWK=
RMG-I M1nPfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYLtUoRTUUN3ME21MlY2QTR{IN88US=> MULTRW5ITVJ?
HD-MY-Z Ml7xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVTJR|UxRTVwNki1PFEh|ryP MnPGV2FPT0WU
HCC1187 NYTlOmc5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWjreZNMUUN3ME21MlcyODN{IN88US=> MofqV2FPT0WU
HCC2998 M1u3eWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlzBTWM2OD13LkexPFc4KM7:TR?= NYfFbploW0GQR1XS
RT-112 NGPmWIlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIfIepRKSzVyPUWuPVAzPDVizszN MX7TRW5ITVJ?
CAKI-1 MnnnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXTLVGdMUUN3ME22MlAxQTh3IN88US=> M165bXNCVkeHUh?=
HDLM-2 MoroS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYXJR|UxRTZwMEG0PFkh|ryP NXzud2JzW0GQR1XS
TE-10 M1zKUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVX1UGh[UUN3ME22MlA1QDN|IN88US=> Mn:1V2FPT0WU
KE-37 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{j3[2lEPTB;Nj6wO|I2QCEQvF2= M3\LOnNCVkeHUh?=
RCM-1 NVW0W4VqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYKwdVBUUUN3ME22MlA4PzF7IN88US=> NW\GSJFJW0GQR1XS
Mewo M4XWWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWjySnh7UUN3ME22MlEyPDh5IN88US=> MXTTRW5ITVJ?
L-428 M{jrVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml7KTWM2OD14LkG4NFM4KM7:TR?= NVfZToV4W0GQR1XS
8-MG-BA MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NETxfIVKSzVyPU[uNVk{PzlizszN M2XFWXNCVkeHUh?=
SK-MEL-28 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MU\JR|UxRTZwMkC5Olch|ryP NEG5eYNUSU6JRWK=
VA-ES-BJ M2\Y[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXnLNmROUUN3ME22MlMxPDZ{IN88US=> MW\TRW5ITVJ?
CAL-120 MkPsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlHuTWM2OD14LkS1NVYzKM7:TR?= NFjoSVlUSU6JRWK=
GAMG MmTaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnHtTWM2OD14LkWwOlIh|ryP MYrTRW5ITVJ?
MSTO-221H NX;qfmFHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVrJR|UxRTZwNUGxO{DPxE1? MUDTRW5ITVJ?
GCT M1XNdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYLJR|UxRTZwNUOzPFch|ryP MljLV2FPT0WU
SH-4 NUPSUIhYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX;HPYY2UUN3ME22MlU5QDR2IN88US=> NWLPfGV{W0GQR1XS
Calu-3 NYrZZpU3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NILUT5hKSzVyPU[uOlU2PjhizszN NEPQ[HBUSU6JRWK=
SK-HEP-1 NYfmR|RpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGPvZm1KSzVyPU[uO|E4PTNizszN NV;hVY17W0GQR1XS
PFSK-1 M1zOPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHOwNYxKSzVyPU[uO|M1PzhizszN Ml3DV2FPT0WU
NB12 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVL3ZYZYUUN3ME22Mlc5PDB2IN88US=> MVTTRW5ITVJ?
A388 NHPufmRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEW1dm5KSzVyPU[uPFk2OiEQvF2= M3fu[3NCVkeHUh?=
KLE MmD1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYDJR|UxRTZwOU[1NVYh|ryP MV;TRW5ITVJ?
TE-11 MmfLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2K0[mlEPTB;Nz6wOFk3PyEQvF2= MYrTRW5ITVJ?
NCI-H2405 M4TOVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH\EXYlKSzVyPUeuNFg{QTNizszN MlyxV2FPT0WU
OVCAR-4 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3rWU2lEPTB;Nz6xOVM2KM7:TR?= NVX3NWhbW0GQR1XS
KP-N-YS M1TEb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mo\YTWM2OD15LkG4NVQh|ryP NET4N2lUSU6JRWK=
NOS-1 NUHCZ|BxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2fWTGlEPTB;Nz6yOVgzQCEQvF2= NWC0Nmk4W0GQR1XS
DMS-114 NWXtNFdnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEnqPFJKSzVyPUeuNlk3OzFizszN MlWwV2FPT0WU
NCI-H2030 NUHscYRNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml;yTWM2OD15LkOzOlk5KM7:TR?= NYPhOJFjW0GQR1XS
HEL M3nKS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUXhSIJ3UUN3ME23MlM4PTJzIN88US=> M2XQcHNCVkeHUh?=
CAL-62 NEf4OIZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYLJR|UxRTdwM{m4OlYh|ryP M1;wSnNCVkeHUh?=
COLO-668 M{TPfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3PzWmlEPTB;Nz60N|k3PyEQvF2= M2jMXHNCVkeHUh?=
SKG-IIIa M3;vRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEXjTFJKSzVyPUeuOFU{QDFizszN MnvsV2FPT0WU
HCC1419 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX;pUG9WUUN3ME23MlQ3QDN|IN88US=> MYrTRW5ITVJ?
OS-RC-2 NELZToVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFHhSVdKSzVyPUeuOFk5QTRizszN MlmzV2FPT0WU
COLO-829 NWjxdFdZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn;JTWM2OD15Lk[2OFY{KM7:TR?= MkXxV2FPT0WU
NB13 NFnBWXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWrJR|UxRTdwOEK2Olch|ryP M4fQNXNCVkeHUh?=
DK-MG M{\nNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{PGV2lEPTB;Nz64N|IxOSEQvF2= MlKwV2FPT0WU
SHP-77 NXuwPWM5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnKxTWM2OD15Lki2OlU{KM7:TR?= Mk\jV2FPT0WU
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SW948 MmTLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGe1U5JKSzVyPUO4Mlg6PDlizszN MYnTRW5ITVJ?
MFM-223 NXq5OohKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHvI[YZKSzVyPUO4Mlk1OzFizszN NEnzNVZUSU6JRWK=
PAN1 NX;ocIpTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYjYfmp{UUN3ME2zPE46QTF7IN88US=> M3zZcXNCVkeHUh?=
MZ7-mel M2nhcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX3nVpp[UUN3ME2zPU4yOTl7IN88US=> M2rscHNCVkeHUh?=
COLO-679 MlL0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkDzTWM2OD1|OT65O|A5KM7:TR?= M2DRUnNCVkeHUh?=
SW872 NUjCW4M3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3S4bGlEPTB;NECuNlA{OSEQvF2= MW\TRW5ITVJ?
HT-1197 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVTMbVhvUUN3ME20NE4zQDZ7IN88US=> MV;TRW5ITVJ?
ES6 MoDRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUTJR|UxRTRyLkS1OVIh|ryP NHLCdGZUSU6JRWK=
SNU-387 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2XhTWlEPTB;NECuPFI{QCEQvF2= NIHyUHhUSU6JRWK=
T47D NH75PHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIm5RXZKSzVyPUSxMlAyQTJizszN NF7RcHRUSU6JRWK=
G-402 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYHJR|UxRTRzLkC4NFkh|ryP NYLOSpVpW0GQR1XS
MZ1-PC M1PidWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlzFTWM2OD12MT64NUDPxE1? MYPTRW5ITVJ?
SW1417 NXzoT3lXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWfJR|UxRTR{LkOwN{DPxE1? NVPpPI5CW0GQR1XS
RERF-LC-MS M4TqWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEXBSmpKSzVyPUSyMlUxQDdizszN MmHEV2FPT0WU
SF539 M4DIemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NULsSGw6UUN3ME20Nk42PTJ7IN88US=> MkTYV2FPT0WU
ESS-1 NWXuSGZGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGT1XWRKSzVyPUSzMlU2PThizszN NXjMUmJPW0GQR1XS
RO82-W-1 M3vCOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1P0UGlEPTB;NEOuOlE2OyEQvF2= Mn[wV2FPT0WU
S-117 M3;s[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2XuOWlEPTB;NEOuOlk2QCEQvF2= NV7MTYxQW0GQR1XS
SW756 NIfw[3JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlnpTWM2OD12Mz64NlQzKM7:TR?= MVTTRW5ITVJ?
CP66-MEL M{Ti[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlrVTWM2OD12NT63NVQ{KM7:TR?= NFvYO|BUSU6JRWK=
H-EMC-SS MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHrOUpJKSzVyPUS1Mlc3QTNizszN NYnvSFlOW0GQR1XS
NCI-H2052 M4m2fmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVPJR|UxRTR3LkmwNlch|ryP NHLZXYtUSU6JRWK=
SW684 NX3mPFE{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4jw[GlEPTB;NEeuNFA5OSEQvF2= MV;TRW5ITVJ?
HCC1569 NV71Z444T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYjEfnpNUUN3ME20O{4yPjV6IN88US=> NUjJNVBkW0GQR1XS
KGN MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUTJR|UxRTR5LkOzNVEh|ryP NHHPPJJUSU6JRWK=
LCLC-103H MkP1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NE\ydnFKSzVyPUS3MlQ1ODRizszN M2\hUHNCVkeHUh?=
SW780 NYCxVlhmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV3JR|UxRTR5Lk[zOVEh|ryP NXHzdGtCW0GQR1XS
NCI-H1703 NVHNdJc2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M33RZWlEPTB;NEiuN|M2OiEQvF2= MW\TRW5ITVJ?
HCC1395 M3TTcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVzJR|UxRTR6Lke0N|Ih|ryP NWPBZlc4W0GQR1XS

... Click to View More Cell Line Experimental Data

In vivo Gefitinib (100 mg/kg) improves the anti-tumor effect of radiotherapy in LoVo tumor xenografts. [3] Gefitinib treatment of nude mice bearing established human GEO colon cancer xenografts reveals a reversible dose-dependent inhibition of tumor growth because GEO tumors resumes the growth rate of controls at the end of the treatment. [4]

Protocol

Cell Research:[1]
+ Expand
  • Cell lines: NR6, NR6M and NR6W cells
  • Concentrations: 0-2 μM
  • Incubation Time: 72 hours
  • Method: Exponentially growing cells including NR6, NR6M, NR6M and NR6W cells are seeded in sextuple in 96-well plates at a concentration of 2000 cells/well, allowed to adhere and subsequently washed in PBS and incubated overnight in medium containing 0.5% FCS. Cells are then treated with varying concentrations (0-2 μM) of Gefitinib or the solute control DMSO and EGF. The optimal EGF concentration for inducing proliferation of NR6wtEGFR and NR6W cells has previously been determined and hence NR6wtEGFR and NR6W cells are supplemented with 10 nM and 0.1 nM EGF, respectively. EGF is not added to NR6 and NR6M cells. After 72 hours the amount of cells are measured by performing a MTT proliferation assay.
    (Only for Reference)
Animal Research:[3]
+ Expand
  • Animal Models: Female nude mice (cba nu/nu) aged 8–10 weeks are intra-dermal injected with LoVo cells.
  • Formulation: 0.5% polysorbate
  • Dosages: 100 mg/kg
  • Administration: Once daily by oral administration (0.1 mL/10 g body weight) for 14 days
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 89 mg/mL (199.14 mM)
Ethanol 4 mg/mL (8.95 mM)
Water Insoluble
In vivo Add solvents individually and in order:
5% DMSO+corn oil
2.5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 446.90
Formula

C22H24ClFN4O3

CAS No. 184475-35-2
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03050164 Completed Bioequivalence Study Hunan Kelun Pharmaceutical Co., Ltd. September 8, 2016 Phase 1
NCT02856893 Not yet recruiting NSCLC European Organisation for Research and Treatment of Cancer - EORTC|AstraZeneca March 2017 Phase 2
NCT02976116 Recruiting NSCLC Hutchison Medipharma Limited December 2016 Phase 2
NCT02951637 Not yet recruiting Lung Adenocarcinoma Shanghai Chest Hospital|Ruijin Hospital|Changhai Hospital December 2016 Phase 2
NCT02930954 Not yet recruiting Non-small-cell Lung Cancer Caicun Zhou|Tongji University November 2016 Phase 2
NCT02929693 Enrolling by invitation Cancer Shanghai University of Traditional Chinese Medicine|Shanghai Chest Hospital|Shanghai Pulmonary Hospital, Shanghai, China October 2016 Phase 3

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    I try to dissolve Gefitinib (ZD1839) in 0.5% methylcellulose+0.2% Tween 80 for in vivo experiments. However, when I prepare the mixture, it forms precipitates. Exists another way to dissolve completely the compound?

  • Answer:

    S1025 can be dissovled in both 5% DMSO+corn oil and 5% DMSO+30% PEG 300+5% Tween 80+ddH2O at 2.5 mg/ml clearly. It dissolves in DMSO not readily, please sonicate and warm it in water bath at about 45 degree, and dissolve it clearly first. Then add other solvent.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID