Gefitinib (ZD1839)

Catalog No.S1025

Gefitinib (ZD1839) Chemical Structure

Molecular Weight(MW): 446.90

Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.

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Cited by 91 Publications

12 Customer Reviews

  • Perturbation of EGFR by its ligand EGF and gefitinib (ZD-1839 Iressat; inhibits EGFR) produces opposite responses in the predicted EGFR target genes SOCS2 and NR2E1.

    Mol Syst Biol 2011 7, 486. Gefitinib (ZD1839) purchased from Selleck.

    Effects of shRNA-mediated RECK depletion on EGFR signaling in MEFs. (a) Immunoblotting (IB) of the indicated proteins in wild-type MEFs transduced with the indicated shRNAs and cultured for 48 h in the presence of 0.1% dimethyl sulfoxide (DMSO; vehicle) or 1 mM gefitinib (S1025, Selleck Chemicals,Houston, TX, USA). (b) Immunoprecipitation from whole lysates of cells from panel a with an anti-phosphorylated tyrosine (pTyr) antibody. Precipitates from 500 mg protein in whole cell lysates (upper) or 20 mg protein in whole cell lysates (lower) were analyzed by IB with anti-EGFR antibody.

    Oncogene 2010 30, 737-750. Gefitinib (ZD1839) purchased from Selleck.

  • (B–C) LNCaP (B) and LNCaP-AI (C) cells were transiently transfected with sPLA2-IIa(-800)-Luc (0.5 μg). The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 lM) and Bortezomib (20 μM) without or with EGF (100 ng/ml) for 24 h. Luciferase assay was performed according to a standard protocol with Renilla luciferase as an internal control. Data are presented as the mean (±SD) of duplicate values of a representative experiment that was independently repeated for five times.

    Carcinogenesis 2010 31, 1948–1955 . Gefitinib (ZD1839) purchased from Selleck.

    LNCaP-AI cells were starved in 1% stripped medium for 24 h. The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 μM) and Bortezomib (20 μM) for 24 h. Cell culture medium was collected from each sample and subjected to ELISA for sPLA2-IIa. The condition medium samples were diluted 10 times for ELISA. Average of duplicate samples was converted to nanogram per milliliter against standard curve. The data represent one of five repeated experiments.

     

     

    Carcinogenesis 2010 31, 1948–1955. Gefitinib (ZD1839) purchased from Selleck.

  • Susceptibility of lung cancer cells to cytolytic activity of NK-92 cells after treatment with EGFR inhibitors. Three lung cancer cells were untreated (open circle) or treated with 10 μM erlotinib or gefitinib (black filled square or black filled triangle) for 24 hours. The lung cancer cells were cocultured with NK-92 cells at indicated effector to target ratio (E:T ratio). To determine the specificity of NKG2D-mediated cytolysis of lung cancer cells, NK-92 cells were preincubated with blocking mAb against NKG2D before the assay (gray filled square and triangle) and correspond-ing isotype mAb (open square and triangle).

    J Immunother 2011 34(4), 372-81. Gefitinib (ZD1839) purchased from Selleck.

    Suppression of EGFR signaling by Gefitinib is dose dependent and non- toxic at millimolar concentrations.Results from plaque reduction tests of Hep2 cells infected with VACV(12.5pfu/well) treated with a serial dilution of Gefitinib (1000-0.01 m) and analyzed for (A)proliferation as indicator of cytotoxicity,(B) IC50 of plaque size inhibition, (C)dose dependent inhibition of EGFR-ERK1/2signaling and VACV proteins by Western blot analysis (pEGFR detection was obtained from a different experiment with identicals ettings) and (D)dose dependent inhibition of orthopoxvirus genome replication by real-time PCR.Untreated VACV infectedcells and uninfected cells are shown as controls in the right panel.

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

  • A circular zone of EGFR activated but uninfected cells facilitate virus induced plaque development , efficiently blocked by Gefitinib. (A)Immun of luorescent staining of aplaque reduction tests with Hep2 cells 24,48,72 and 96 h after VACV infection (6.25pfu/chamber). VACV infected cells(green) were visualized by human IgG directed to orthopoxviruses (VIG) detected with a secondary FITC labelled antibody. Uninfected cells with activated EGFR signaling(red) are detected with a secondary Cy3labelled antibody. Positive cells for both , VACV and phosphorylated EGFR are shown in yellow(merge). Cellular nuclei are counterstained with DAPI. (B)Immunofluorescent staining of a plaque reduction test as described for (A) and treated with three different Gefitinib concentrations (1000 nM, 1 nM, and 0.01 nM) . The test was analyzed 96h afterVACV infection.Uninfected cells are shown as controls. Magnification100×.(For interpretation of the references to color in this figure legend , the reader is referred to the web version of the article.)

     

     

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

    The EGF-dependent increased association of PCM1 and AZI1 requires EGFR kinase activity. ZD1839 (Iressa)—EGFR tyrosine kinase inhibitor.

    Mol Biosyst 2011 7, 3223-33. Gefitinib (ZD1839) purchased from Selleck.

  • Reduction of GPER expression in HCC1806 cells after treatment with gefitinib. (A) Concentration dependence. HCC1806 cells were treated with 50-500 nM gefitinib for 48 h. GPER was detected by western blotting. (B) Time dependence. HCC1806 cells were treated with 200 nM gefitinib for 24-96 h. ap<0.05 vs. control; bp<0.001 vs. control. Mean values and SE of three independent experiments are shown. (C) Representative western blotting of concentration dependence. (D) Representative western blotting of time dependence

    Oncol Rep, 2017, 37(2):1212-1218. Gefitinib (ZD1839) purchased from Selleck.

    Inhibition of signaling pathway activation in lung tumor cell lines by kinase inhibitors. Lung tumor cells were cultured in 10% FBS until reaching ∼80% confluence and then the cells were starved in serum-free medium for overnight, followed by 4-hour treatment with the inhibitors. Cell lysates were then prepared and used for determination of the pathway activation signals by the CEER assay.

    Int J Proteomics 2011 215496. Gefitinib (ZD1839) purchased from Selleck.

  • Breast cancer cells were pretreated with 100ng/ml EGF for 15 min and then treated with the indicated concentrations of  Gefitinib for 24 hours.

     

     

    2010 Dr. Zhang of Tianjin Medical University. Gefitinib (ZD1839) purchased from Selleck.

    MTT assay result. 0-500nM Gefitinib treated with H1299, H358, H25 and HCC827 cell lines by 72h, Proliferation rate was tested.

     

     

    2010 Dr. Vicky Tin from University of Hong Kong. Gefitinib (ZD1839) purchased from Selleck.

Purity & Quality Control

Choose Selective EGFR Inhibitors

Biological Activity

Description Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.
Features A potent EGFR tyrosine kinase inhibitor.
Targets
Tyr1173 (NR6W cells) [1] Tyr1173 (NR6wtEGFR cells) [1] Tyr992 (NR6wtEGFR cells) [1] Tyr992 (NR6W cells) [1]
26 nM 37 nM 37 nM 57 nM
In vitro

Gefitinib effectively inhibits all tyrosine phosphorylation sites on EGFR in both the high and low-EGFR-expressing cell lines including NR6, NR6M and NR6W cell lines. The phosphorylation sites Tyr1173 and Tyr992 are less sensitive requiring higher concentrations of Gefitinib for inhibition. Gefitinib effectively blocks the phosphorylation of PLC-γ, with IC50 of 27nM, in NR6W cells. The NR6wtEGFR and NR6M cell lines has low levels of PLC-γ phosphorylation but the level in the NR6M cell line is more resistant to inhibition by Gefitinib with IC50 of 43 nM and 369 nM, respectively. Gefitinib inhibits Akt phosphorylations, with IC50 of 220 and 263nM, in the low-EGFR- and -EGFRvIII-expressing cell lines, respectively. Gefitinib in the dose range from 0.1 to 0.5μM significantly facilitates, rather than abrogates, colony formation of NR6M cells. However, at a concentration of 2 μM Gefitinib completely blocks NR6M colony formation. Gefitinib rapidly and in a dose-dependent manner inhibits EGFR and ERK phosphorylation up to 72 hours after EGF stimulation in both the high- and low-EGFR-expressing cell lines. [1] Gefitinib is the monolayer growth of these EGF-driven untransformed MCF10A cells with an IC50 of 20 nM. [2] The combination of Gefitinib (0.2 μM and 0.5 μM) with irradiation lead to a significant growth inhibition in LoVo cells, compared with radiation alone.. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KB M3fi[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYnXUGVQUUN3ME2wMlA2PCEQvF2= M2jFPVE3PTF4NEez
NIH3T3 MoHQSpVv[3Srb36gRZN{[Xl? MXKwMlIh|ryP NGPFNpYzKGh? M1v1bGROW09? NVHSNHZWUW6qaXLpeIlwdiCxZjDFS2Yhe3SrbYXsZZRm\CCqdX3hckBmemKEMTDheZRweGixc4Doc5J6dGG2aX;uJJdqfGhiSVO1NEBw\iByLkCxOFQh|ryP MYmxOlQ5ODJ6NB?=
COS7 NHLSUXNHfW6ldHnvckBCe3OjeR?= NYfiTJV7QTZizszN M1\YXGlvcGmkaYTpc44hd2ZiaIXtZY4hUkGNMjDWOlE4TiCvdYThcpQh\XiycnXzd4Vl NEWxU20yPzF5OEeyNi=>
A549 MmnWR5l1d3SxeHnjJGF{e2G7 M4CxS|k3KGh? NVv4UGZDTE2VTx?= MmTETWM2OD1zMz61PUDPxE1? MXSxPFMyOzhyNx?=
K562 MWjDfZRwfG:6aXOgRZN{[Xl? NHTTWIc6PiCq MUfEUXNQ NEfwVIdKSzVyPUmuN|Yh|ryP M{XhOlE5OzF|OEC3
MDA-MB-231 MlXnR5l1d3SxeHnjJGF{e2G7 M{W0NFk3KGh? NFzX[3FFVVOR M4L0SWlEPTB;M{[uOlEh|ryP MmTjNVg{OTN6MEe=
MOLT4 NH\LNIhEgXSxdH;4bYMhSXO|YYm= MVO5OkBp M1rDfmROW09? NWLYeY9[UUN3ME2xOU4xOiEQvF2= M4W2WlE5OzF|OEC3
MCF7 NVPVT3BjS3m2b4TvfIlkKEG|c3H5 MWC1NEDPxE1? MUW3NkBp MYjJR|UxRTF{LkC1JO69VQ>? M{faNFE5PzdzOEG5
BT474 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUP1cJNvPzJiaB?= NUTzNI5zSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDCWFQ4PCClZXzsd{BwfmW{ZYjwdoV{e2mwZzDFVmJjOiC5aYToJGVEPTBib3[gNE4{KM7:TR?= M3TI[VE6ODJ6NEK0
HN5 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mk\1O|IhcA>? NEjnZZpCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjOOUBk\WyuczDveoVz\XiycnXzd4lv\yCHR1\SJJdqfGhiRVO1NEBw\iByLkCxJO69VQ>? MmjuNVkxOjh2MkS=
BA/F3 NVPhb3F1S3m2b4TvfIlkKEG|c3H5 NVfFUGh4PzJiaB?= M3;uSWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJGJCN0Z|IHPlcIx{KGW6cILld5NqdmdiRVfGVkBNQDV6UjDteZRidnRid3n0bEBKSzVyIH;mJFAvODFyODFOwG0> NEG3OVMyQTJ|OUKyPS=>
NIH3T3 Mn;nS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWq3NkBp M36zW2dzd3e2aDDpcohq[mm2aX;uJI9nKG2xdYPlJG5KUC9|VEOgZ4VtdHNiZYjwdoV{e2mwZzDldmIzKGenbnWge4l1cCCLQ{WwJI9nKDBwNUSg{txO MVexPVY3PTN5Nx?=
MCF7 MX M375VGZ2dmO2aX;uJGF{e2G7 NYnkZ2lxOzBibXnu NXPJZo9XUW6qaXLpeIlwdiCxZjDCR3JRKGW6cILld5Nm\CC5aYToJGlEPTBib3[gNU4yKM7:TR?= MkjkNVk6OzJ7NkC=
A431 M{LOdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{ThZlExKM7:TR?= NFW2T444OiCq NIjobXlIUTVyPUCuOVE1KM7:TR?= NWDKcVFUOTl7Nkm0OlU>
A431 MULLbY5ie2ViQYPzZZk> NVvSbnNyOC5zIN88US=> MoTQNUBp MUnJcohq[mm2aX;uJI9nKEWJRj3pcoR2[2WmIFXHSnIheGixc4Doc5J6dGG2aX;u MXuxPVk3QTR4NR?=
A431 NUPXSFhmU2mwYYPlJGF{e2G7 MmPCNVAxKM7:TR?= NGPCNooyOCCvaX6= NEXHbIZRem:ub37n[YQhcW6qaXLpeI9zgSCjY4Tpeol1gSCxZjDFS2YucW6mdXPl[EBGT0[UIIDoc5NxcG:{eXzheIlwdg>? MoHkNVk6Pjl2NkW=
Sf9 NV23V41uU2mwYYPlJGF{e2G7 M4TwSVExKG2rbh?= M2DObWROW09? MV;Jcohq[mm2aX;uJI9nKEWJRmKg[ZhxemW|c3XkJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gdoVk\XC2b4KgZZV1d3Cqb4PwbI9zgWyjdHnvckB4cXSqIFnDOVAhd2ZiMD6wN|Mh|ryP NGq0fIwzODB3NkSyOS=>
NCI-H1975 MkXkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUO1JO69VQ>? NHS5RlQ4OiCq NFTJ[HJKSzVyPUiuNlYh|ryP M3P5WlIxOTVzNkew
NCI-H1975 NEPaV|ZMcW6jc3WgRZN{[Xl? M33mT|ExKM7:TR?= M3KwUmlvcGmkaYTpc44hd2ZiRVfGVkBifXSxcHjvd5Bpd3K7bHH0bY9v NUXFe3diOjBzNUG2O|A>
NCI-H1975 MkHGSpVv[3Srb36gRZN{[Xl? MV6xNEDPxE1? M{S2RWlvcGmkaYTpc44hd2ZiRWLCRlIh[XW2b4Doc5NxcG:{eXzheIlwdg>? NEn3Uo8zODF3MU[3NC=>
NCI-H1975 MWHGeY5kfGmxbjDBd5NigQ>? MXy1JO69VQ>? M1\iVVQ5KGh? M2Htcmlv\HWldHnvckBw\iCjcH;weI9{cXNiYYPz[ZN{\WRiYYOgZYN1cX[jdHnvckBw\iClYYPwZZNmNTN? NX3XfIpROjBzNUG2O|A>
A375 NXSz[5BmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmHvOFghcA>? NYrOVVNHUUN3ME2yNE42PSEQvF2= MlPQNlA{ODR3M{e=
SMMC7721 NX3YT3A6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVLseJI4PDhiaB?= M1;LOmlEPTB;M{OuNVIh|ryP NYjMXIdNOjB|MES1N|c>
DU145 M4rvUGN6fG:2b4jpZ{BCe3OjeR?= MX61JIQ> MX\EUXNQ NI[3cnBKSzVyPUSyMlE4KM7:TR?= NHnwUmQzODR4NkW1OS=>
MIAPaCa2 MX;DfZRwfG:6aXOgRZN{[Xl? MYK1JIQ> MVTEUXNQ MVfJR|UxRTVyLk[yJO69VQ>? MXiyNFQ3PjV3NR?=
PANC1 NI\zV2REgXSxdH;4bYMhSXO|YYm= M1O4eFUh\A>? MlTtSG1UVw>? NXvNT2hIUUN3ME20NE41OiEQvF2= NFfJdmgzODR4NkW1OS=>
PC9 M1:5TWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGLv[FVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGVITlJvZHXmbYNq\W62IHj1cYFvKFCFOTDj[YxteyC5aYToJGVEPTBib3[gNE4xOTdizszN MlqxNlEzODh6MEK=
PC9 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUX1XnVJSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBoem:5dHitdoV{cXO2YX70JIh2dWGwIGDDPUBk\WyuczDlfJBz\XO|aX7nJGVITlJiRUe0On9CPzVyL2S3PVBOKG23dHHueEB4cXSqIFXDOVAhd2ZiNzFOwG0> MmHLNlEzODh6MEK=
PC3 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIiweJQyOCEQvF2= M{LkWlczKGh? NWqzbpJFTE2VTx?= M1fye2lEPTB;Nz60JO69VQ>? MoT0NlE{PTN3NE[=
MDA-MB-468 NHvzToFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M33RVFExOCEQvF2= MkDxOFghcA>? M{X1b2ROW09? MnrVRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdIm= NILzcnQzOTR{OU[zNi=>
Plasmodium falciparum MUPxTHRUKHC{b3\pcIlv\yCob4KgbY5pcWKrdH;yd{Bw\iCSbHHzcY9lcXWvIH\hcINqeGG{dX2gdJJwdGmoZYLheIlwdg>? NG\LPJEzOThzN{C0OS=>
NCI-H1993 MnHiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWC3NkBp MYLEUXNQ NX2wUI16UUN3ME2wMlEh|ryP MXeyNVkyPjR|Mx?=
NCI-H292 MlPHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUW3NkBp NHf4OIZFVVOR M1Tvb2lEPTB;MD6wNkDPxE1? MUOyNVkyPjR|Mx?=
A431 NGPPVHVMcW6jc3WgRZN{[Xl? NG\ydHozKGh? M4rTcmROW09? NES1d4lKdmirYnn0bY9vKG:oIFXHSnIheGixc4Doc5J6dGG2aX;uJIlvKEWJRj3zeIlufWyjdHXkJIh2dWGwIFG0N|Ehf2m2aDDJR|UxKG:oIECuNFAyKM7:TR?= NGfNO|QzOjFyMUGzNi=>
MCF7 M2\icmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXPPelAyOTBywrFOwIcwdUx? M1LRNVQ5KGh? NX[3dVRETE2VTx?= M1PmT2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDveoVz\XiycnXzd4lv\yCHR1\SJIdmdmVid3n0bEBKSzVyIH;mJFEzNjB3IN88US=> NFn6XJEzOjFzOUGzNC=>
BT474 NVTwPHZtS3m2b4TvfIlkKEG|c3H5 MV6xNEDPxE1? NYHCeGp4PDhiaB?= MorGR5l1d3SxeHnjbZR6KGGpYXnud5Qhe2W{dX2td5Rien[nZDDoeY1idiCEVES3OEBk\Wyucx?= MkjNNlIyOzh|MEi=
MCF7 NUnBWFVJS3m2b4TvfIlkKEG|c3H5 M{\JSlExKM7:TR?= NFqwfXA1QCCq NFf6UoREgXSxdH;4bYNqfHliYXfhbY5{fCC|ZYL1cU1{fGG{dnXkJIh2dWGwIF3DSlch[2WubIOgc5Zmei2neIDy[ZN{cW6pIFXyZmIzKGOnbHzz NIO4OpUzOjF|OEOwPC=>
HCC827 Ml7tT4lv[XOnIFHzd4F6 M2DwfFAvOTJ3IN88US=> M4PoO|I1KGh? MoO4SG1UVw>? NG\zeWtKdmirYnn0bY9vKG:oIFXHSnIh\GWuIFW3OFYuSTd3MDDteZRidnRiWUGwOlgh[XW2b4Doc5NxcG:{eXzheIlwdg>? Mn3wNlI{Ozl|NEK=
HCC827 NUXsXlRLU2mwYYPlJGF{e2G7 NVf3WJl2OC5zMkWg{txO M3jRT|I1KGh? NGG3flNFVVOR NFXwW4RKdmirYnn0bY9vKG:oIFXHSnIh\GWuIFW3OFYuSTd3MDDteZRidnRvbXXkbYF1\WRiRYLrJJBpd3OyaH;yfYxifGmxbh?= M4fzNVIzOzN7M{Sy
HCC827 M2rQdWtqdmG|ZTDBd5NigQ>? M1;JZlAvOTJ3IN88US=> MYOyOEBp M33pXGROW09? NUT5NZVWUW6qaXLpeIlwdiCxZjDFS2ZTKGSnbDDFO|Q3NUF5NUCgcZV1[W62LX3l[IlifGWmIFHreEBxcG:|cHjvdplt[XSrb36= MoLmNlI{Ozl|NEK=
A431 M1OzXWtqdmG|ZTDBd5NigQ>? NYHKTpcxOTByIN88US=> M{nxNlMxKG2rbh?= MnXCTY5pcWKrdHnvckBw\iCHR1\SJIRmemm4ZXSg[pJwdSCqdX3hckBCPDNzIHPlcIwhdWWvYoLhcoUh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBxd2y7LVfseU1VgXJicHjvd5Bpd3K7bHH0bY9vKHerdHigTWM2OCCxZjCxMlA5KM7:TR?= M1vQb|IzPjl6N{iy
H460 M3vzOmN6fG:2b4jpZ{BCe3OjeR?= M1jEe|czKGh? NFj5bGxKSzVyPUWuOVkh|ryP MkHONlI4Ojd2NEm=
HT-29 NGrWcW1EgXSxdH;4bYMhSXO|YYm= NYnJ[I1NPzJiaB?= MnPLTWM2OD1|LkO2JO69VQ>? MW[yNlczPzR2OR?=
SGC7901 MXHDfZRwfG:6aXOgRZN{[Xl? M4PsTlczKGh? NFvvWlFKSzVyPUGwMlI3KM7:TR?= NI\ic2QzOjd{N{S0PS=>
A498 MmjDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEF2OUigZ4VtdHNiZYjwdoV{e2mwZzDFS2ZTKGGwZDDTVmMhf2m2aDDJR|UxKG:oIEiuOkDPxE1? M4DjflIzQDF6OES4
Calu3 NIj4XVBEgXSxdH;4bYMhSXO|YYm= MXyxNEDPxGdxbVy= NUHvUnFpPzJiaB?= MlvYTWM2OD1zLk[g{txO NGPIbHEzOzFzNkG2PC=>
FADU MmfRR5l1d3SxeHnjJGF{e2G7 MYqxNEDPxGdxbVy= MX:3NkBp M33kZmlEPTB;MD6wOUDPxE1? MnnkNlMyOTZzNki=
HCC827 NFq1W3FEgXSxdH;4bYMhSXO|YYm= M2rFeVExKM7:Zz;tUC=> NFe4V5E4OiCq NX7CSGl4UUN3ME2wMlAxOTVizszN MVmyN|EyPjF4OB?=
PC9 NXGwfGg5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2nDW|ExKM7:Zz;tUC=> MVi3NkBp NY\BN|ZPUUN3ME2wMlAxOTRizszN NF[0Z5YzOzFzNkG2PC=>
Sf9 MmDQT4lv[XOnIFHzd4F6 NGjo[WoyKGh? MnnmSG1UVw>? MULJcohq[mm2aX;uJI9nKGi3bXHuJGdUXC22YXfn[YQhTUeIUjDMPFM1WiCvdYThcpQh\XiycnXzd4VlKHerdHigTWM2OCCxZjCwMlAyPSEQvF2= NID5WXozOzZzMU[5NS=>
16HBE14o- M1\nTGN6fG:2b4jpZ{BCe3OjeR?= MlnLNVAh|ryP NHPiOY04OiCq NFvtVYxFVVOR NFHBTnpEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckAyPkiERUG0c{0h[2WubIOgbIFz[m:{aX7nJJdqdGRidInw[UBGT0[UIHHzd4V{e2WmIHHzJIdzd3e2aDDpcohq[mm2aX;uJJdqfGhiSVO1NEBw\iBzMj63NUDPxE1? MWeyN|Y3QDR2MR?=
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MDCK2 NYXYdWJtTnWwY4Tpc44hSXO|YYm= NVzQcGltOS56IN88US=> NGK4RVg4OiCq M3O2dGROW09? MV3Jcohq[mm2aX;uJI9nKGi3bXHuJGJEWlBiZYjwdoV{e2WmIHHzd4V{e2WmIHHzJGhw\WOqc4SgN|M{PDJiYXPjeY12dGG2aX;uJJdqfGhiSVO1NEBw\iB|LkG5JO69VQ>? MmjJNlQyQDR{MUO=
A431 MWDDfZRwfG:6aXOgRZN{[Xl? M3XVRlczKGh? NVXNWGJyTE2VTx?= MoS0R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVQ{OSClZXzsd{BmgHC{ZYPzbY5oKEWJRmKge4l1cCCLQ{WwJI9nKDBwNUWg{txO NXzqUWo4OjR2MUGxNlM>
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HepG2 NUnJdHpDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NE\ZelczPCCq NFHrSYJFVVOR NFnmToNKSzVyPU[uOFIh|ryP NGDZPHIzPDd|MUK4NS=>
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HCC827 NYm0UFlxTnWwY4Tpc44hSXO|YYm= MlfINE42KM7:TR?= NXnxdlA3OiCq MmDkSG1UVw>? NWHRXVBW\G:nczDuc5QhcW6qaXLpeEBkNU2ndDDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFHLWEBxcG:|cHjvdplt[XSrb36= MmHFNlM6QTN|Mki=
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NCI-H1975 M4iycWZ2dmO2aX;uJGF{e2G7 NUfN[4V[OTBizszN MnnzPEBp M3XyUWROW09? MmnF[I9meyCwb4SgbY5pcWKrdHmgSWdHWiCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4hTVKNIIDoc5NxcG:{eXzheIlwdg>? MmC3NlMyOTZzNki=
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H1975 MmLqT4lv[XOnIFHzd4F6 M2DoRVEh|ryP NF3FUFIzPCCq NIHLfFZFVVOR MV;kc4V{KG6xdDDpcohq[mm2IFXHSnIheGixc4Doc5J6dGG2aX;uJIlvKGi3bXHuJGgyQTd3IHPlcIx{KGijcnLvdolv\yCHR1\SJGw5PTiUL2S3PVBOKG23dHHueC=> NEXyVlczPDZyN{W5NS=>
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HCC827 MWjLbY5ie2ViQYPzZZk> NIjYNFIxNjFzIN88US=> M1XDZVEhcA>? NXvTT4oyTE2VTx?= NWrrW|NJcGG|IH7vJIlzemW4ZYLzbYJt\SCrbnjpZol1cW:wIH;mJGVITlJiYYPz[ZN{\WRiYYOgdoVkd3[ncomgc4YhTUeIUjDheZRweGixc4Doc5J6dGG2aX;u MV:yOFEzPDh7OB?=
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H1975 MX7LbY5ie2ViQYPzZZk> NIHNVVkxNjN|IN88US=> NIjuWXAzKGh? NGq0d5dFVVOR MX7kc4V{KG6xdDDpcohq[mm2IFXHSnIhVDh3OGKvWFc6OE1iZH;1ZoxmKG23dHHueEBie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gSXJMKHCqb4PwbI9zgWyjdHnvci=> NIG2RnczPDF{NEi5PC=>
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H1975 NVj0WoI6U2mwYYPlJGF{e2G7 MlzxNE4yOjVizszN NV;TTVdKOjRiaB?= NUCzbosyTE2VTx?= M1fSUYRw\XNibn;0JIlvcGmkaYSgSWdHWiCOOEW4Vk9VPzlyTTDteZRidnRiWUGwOlgh[XW2b4Doc5NxcG:{eXzheIlwdg>? MWWyNlM{QTN2Mh?=
H1975 M4jWUGtqdmG|ZTDBd5NigQ>? NXm3bJp7OC53IN88US=> M1;UXlI1KGh? NWq0fYM5TE2VTx?= NWnxNJlS\G:nczDuc5QhcW6qaXLpeEBGT0[UIFy4OVhTN1R5OUDNJI12fGGwdD3t[YRq[XSnZDDFdosheGixc4Doc5J6dGG2aX;u MkDJNlI{Ozl|NEK=
H1975 MV\LbY5ie2ViQYPzZZk> M{fJPFAvPSEQvF2= NGnsNWUzPCCq M{fxeGROW09? MoDV[I9meyCwb4SgbY5pcWKrdDDFS2ZTKEx6NUjSM3Q4QTCPIH31eIFvfC2vZXTpZZRm\CCDa4SgdIhwe3Cqb4L5cIF1cW:w NEjKWWczOjN|OUO0Ni=>
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HSC-4 NGfyVlJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml7PTWM2OD14MT61N{BvVQ>? Ml7QV2FPT0WU
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NCI-H292 NGH3XFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3HW[2lEPTB;MU[yMlY3KG6P MlLiV2FPT0WU
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LU-65 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXfJR|UxRTF4Nj62NUBvVQ>? MoHvV2FPT0WU
BV-173 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2rpfGlEPTB;MUi2MlU2KG6P NIXaW5hUSU6JRWK=
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KYSE-140 M3XkOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3TOTWlEPTB;MkC5Mlk3KG6P NGD3O2JUSU6JRWK=
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CAL-72 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml34TWM2OD1{NkiuO|Mhdk1? M3P6SHNCVkeHUh?=
MEG-01 NEnyOJRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NILiXmlKSzVyPUK3Ok44PiCwTR?= NFz0cIFUSU6JRWK=
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NCI-H2170 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYeyPWxmUUN3ME2yMlM1Pzh|IN88US=> M1W4[XNCVkeHUh?=
EW-18 MlnES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1GxTGlEPTB;Mj6zOVQ4OSEQvF2= MoH4V2FPT0WU
KU812 NIW1cVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{PpRmlEPTB;Mj6zOlc4QSEQvF2= M3PCV3NCVkeHUh?=
NY NEX1N4NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXPJR|UxRTJwM{eyO{DPxE1? MYnTRW5ITVJ?
HTC-C3 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHvaXYZKSzVyPUKuOFIzPjdizszN MlL3V2FPT0WU
CFPAC-1 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkXCTWM2OD1{LkSzPFY1KM7:TR?= Mo\IV2FPT0WU
HH NHHEOoFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2qyZmlEPTB;Mj60PVg2PCEQvF2= MoHZV2FPT0WU
MKN7 Ml7tS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYW3dpU3UUN3ME2yMlYzPzR6IN88US=> MWjTRW5ITVJ?
TE-8 MoeyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3\qcmlEPTB;Mj62N|Y2OyEQvF2= M2LLeXNCVkeHUh?=
MC-IXC MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH3jdmlKSzVyPUKuOlQ1PTNizszN NIPRT49USU6JRWK=
DMS-79 NEXPZnVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXLJR|UxRTJwNkmyPFQh|ryP NVzPPWpFW0GQR1XS
Detroit562 M4jzOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEPz[ZhKSzVyPUKuOlk5PDVizszN NGLkToFUSU6JRWK=
HuP-T4 NXO2fJg1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXewVpVTUUN3ME2yMlczQDF6IN88US=> NV;YZlNxW0GQR1XS
QIMR-WIL Ml\lS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmrvTWM2OD1{Lke1PVUzKM7:TR?= MnPyV2FPT0WU
BB65-RCC MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NECxcnJKSzVyPUKuPFA4OTNizszN MX;TRW5ITVJ?
PC-3 M1fmTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2jRcGlEPTB;Mj64N|MyPyEQvF2= MYfTRW5ITVJ?
OVCAR-3 NWXhcVUzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYn2bYtsUUN3ME2yMlg2PTl2IN88US=> M4rUdHNCVkeHUh?=
KYSE-510 NXvnTIk6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1rpemlEPTB;Mj64Olc5PiEQvF2= NYexVGRIW0GQR1XS
HC-1 NV6zemgxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUXXPYM6UUN3ME2yMlk1ODF{IN88US=> Mnf6V2FPT0WU
NCI-H1693 NIPpWZVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoPXTWM2OD1{Lkm1PFk4KM7:TR?= MVXTRW5ITVJ?
DU-4475 MlfWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHfoU4NKSzVyPUKuPVkxPTJizszN MVXTRW5ITVJ?
COLO-680N NXPEVpN5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NW\WbJdEUUN3ME2zMlAxPDl3IN88US=> NGfRR4tUSU6JRWK=
MN-60 MnPSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NULSPHBnUUN3ME2zMlA1QTd2IN88US=> M{i4enNCVkeHUh?=
BCPAP MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVnJR|UxRTNwMEiyPVUh|ryP MX\TRW5ITVJ?
647-V NES4[WVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYfJR|UxRTNwMU[yNkDPxE1? M4ewSHNCVkeHUh?=
HT-1376 MnLOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkjMTWM2OD1|LkKwOVA3KM7:TR?= MliwV2FPT0WU
NCI-H1793 NF25SHVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4G1bWlEPTB;Mz6yNVA6QCEQvF2= NILHc4RUSU6JRWK=
SW1463 NIf4XIZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NI\S[XlKSzVyPUOuNlYyQTJizszN MULTRW5ITVJ?
NCI-H727 M2TtPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2XqRmlEPTB;Mz6zPFU5PCEQvF2= NG\QVVFUSU6JRWK=
LB771-HNC MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF\OU5VKSzVyPUOuN|kyOjZizszN NX;nO2hlW0GQR1XS
D-542MG NYq4c|UxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWTJR|UxRTNwNESyNlIh|ryP MYLTRW5ITVJ?
NTERA-S-cl-D1 MmLHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYCzdXlIUUN3ME2zMlQ1QTV5IN88US=> M2H1V3NCVkeHUh?=
C8166 M{XPUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWTBVWtPUUN3ME2zMlQ3PTJzIN88US=> M2rYcnNCVkeHUh?=
639-V M{nVN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MW\JR|UxRTNwNUe3PVIh|ryP MVHTRW5ITVJ?
TE-1 NH;WOHhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHG2XJJKSzVyPUOuOVkyPjlizszN MkXxV2FPT0WU
OAW-28 MoDGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{TWVWlEPTB;Mz61PVQzPiEQvF2= MXfTRW5ITVJ?
SK-LU-1 NVj1SIQ6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHzNOHhKSzVyPUOuOlA3PDVizszN MWfTRW5ITVJ?
CaR-1 MkHMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVeyNIR3UUN3ME2zMlYyODR6IN88US=> MlTLV2FPT0WU
A2780 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYfHTpk6UUN3ME2zMlY1ODh7IN88US=> MVLTRW5ITVJ?
C2BBe1 M2nSWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVjJR|UxRTNwNkS0NFch|ryP M2TNOXNCVkeHUh?=
SK-MES-1 Mmq2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NULlenpYUUN3ME2zMlY1PjF2IN88US=> NG\OTFlUSU6JRWK=
PANC-10-05 MnX1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MonaTWM2OD1|LkeyNFczKM7:TR?= M3fqNHNCVkeHUh?=
J-RT3-T3-5 M1v2R2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1\HemlEPTB;Mz63OVQxPiEQvF2= MWTTRW5ITVJ?
ES4 NHS5fGlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NE\qNopKSzVyPUOuPFIzQDZizszN M1fIT3NCVkeHUh?=
NCI-H28 NGHRWpNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M37l[2lEPTB;Mz64Nlk3OSEQvF2= MlzOV2FPT0WU
NCI-H1155 M{LrVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIPibnZKSzVyPUOuPFQyPTlizszN NUfCTXRCW0GQR1XS
KURAMOCHI NV;CfVdFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2jqe2lEPTB;Mz64OlA2PiEQvF2= Mke5V2FPT0WU
NCI-H69 NInX[G9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2nKeGlEPTB;Mz65NFE4QCEQvF2= NYfXeXQ4W0GQR1XS
IST-MES1 NFLtcI1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MULJR|UxRTNwOUSwNlkh|ryP MYDTRW5ITVJ?
NH-12 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFW0ZlRKSzVyPUOuPVQyQDdizszN MXLTRW5ITVJ?
JVM-2 M33XZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF7DSnBKSzVyPUOuPVQ4PTJizszN NIGwWXZUSU6JRWK=
EW-16 NGHRbpRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIrpUohKSzVyPUOuPVU2PzdizszN Mo\sV2FPT0WU
NCI-H441 NXn5Sm1kT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFfR[GhKSzVyPUOuPVcxOTRizszN Mn\mV2FPT0WU
RCC10RGB NEnSVXRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYHtfIVPUUN3ME20MlAyOjR4IN88US=> NXv4NWcxW0GQR1XS
TCCSUP NV6yWGRLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIq0SpBKSzVyPUSuNVExPSEQvF2= Ml6zV2FPT0WU
HL-60 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX25WYFoUUN3ME20MlEzOjRzIN88US=> M2nhe3NCVkeHUh?=
EW-11 MkTFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mm\hTWM2OD12LkGzNlYyKM7:TR?= NWHocnY1W0GQR1XS
KARPAS-299 MojjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX3FRnBpUUN3ME20MlMyODF|IN88US=> NV3ifoJnW0GQR1XS
SNU-C2B NIjoNJZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUf5S5pFUUN3ME20MlM1Pjh4IN88US=> MYXTRW5ITVJ?
LU-139 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUXJR|UxRTRwM{eyOFch|ryP NU\4SmF[W0GQR1XS
HCC1937 MmHJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlnXTWM2OD12LkO3PVk{KM7:TR?= MWPTRW5ITVJ?
GR-ST M3HSWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVTJR|UxRTRwNEOxPEDPxE1? NXnQOmdMW0GQR1XS
SK-OV-3 MkLQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGK4cnNKSzVyPUSuOFYxPDJizszN M1vxR3NCVkeHUh?=
A172 NUC1[nl[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEXOZpdKSzVyPUSuOVU2OjFizszN MUfTRW5ITVJ?
NB10 NXO5UHkzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWDQWZBMUUN3ME20MlU6PzhzIN88US=> MmLEV2FPT0WU
P30-OHK MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH;UOHNKSzVyPUSuPFE6OjlizszN MoXuV2FPT0WU
KYSE-520 M3XObmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVXJR|UxRTRwOEiwNVgh|ryP NEK2ToJUSU6JRWK=
KNS-62 NEHZc2RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWDJR|UxRTRwOUC4NFch|ryP MV7TRW5ITVJ?
ES1 NWXi[IZTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mnj3TWM2OD12Lkm2NFM2KM7:TR?= M{\SeHNCVkeHUh?=
KOSC-2 NVK0[414T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV\KPG1QUUN3ME20Mlk6QTV7IN88US=> M2Psd3NCVkeHUh?=
TE-9 MmDDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV;jU2R{UUN3ME21MlAyPTJ3IN88US=> M{naeXNCVkeHUh?=
NCI-H1838 NGHnPFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1nhXGlEPTB;NT6wNlA4PiEQvF2= NGXKSm1USU6JRWK=
KP-N-YN NHPjUmZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGfqNFVKSzVyPUWuNVI2QTFizszN NFXZNYVUSU6JRWK=
ALL-PO NV\rUWVYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2P0eGlEPTB;NT6yNlU5PyEQvF2= M1zne3NCVkeHUh?=
NOMO-1 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWHQfHhQUUN3ME21MlI{OTd4IN88US=> NFHLUJJUSU6JRWK=
CGTH-W-1 MnLyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEXy[JRKSzVyPUWuN|g2QDRizszN Ml7SV2FPT0WU
ATN-1 NYnWUFBVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYTEdWFOUUN3ME21MlQyOTh2IN88US=> NG[5UVRUSU6JRWK=
AGS MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUj1TodiUUN3ME21MlQ6PjF6IN88US=> M3vPTXNCVkeHUh?=
SW13 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWj5WXBZUUN3ME21MlU{OjZ3IN88US=> MVLTRW5ITVJ?
SW1990 NF3Ud|BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2j4WWlEPTB;NT62NlI5PSEQvF2= NWDkRVFwW0GQR1XS
BFTC-905 MlO1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NW\vbG9TUUN3ME21MlY2QDd6IN88US=> M4n0fHNCVkeHUh?=
RMG-I MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFLBe|VKSzVyPUWuOlU6PDJizszN M{ewXHNCVkeHUh?=
HD-MY-Z M4LkUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX;JR|UxRTVwNki1PFEh|ryP NIDUbINUSU6JRWK=
HCC1187 MmLnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWfaeFByUUN3ME21MlcyODN{IN88US=> NVHmXZBXW0GQR1XS
HCC2998 M2Dzd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmiyTWM2OD13LkexPFc4KM7:TR?= NVLZVVMzW0GQR1XS
RT-112 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEmzNWxKSzVyPUWuPVAzPDVizszN MXTTRW5ITVJ?
CAKI-1 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUDJR|UxRTZwMEC5PFUh|ryP MXPTRW5ITVJ?
HDLM-2 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Moq2TWM2OD14LkCxOFg6KM7:TR?= NHj6cZZUSU6JRWK=
TE-10 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYnJR|UxRTZwMES4N|Mh|ryP MoHhV2FPT0WU
KE-37 NF;HPJlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXnF[IdKUUN3ME22MlA4OjV6IN88US=> NEHESYZUSU6JRWK=
RCM-1 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4\XZWlEPTB;Nj6wO|cyQSEQvF2= MWXTRW5ITVJ?
Mewo M13oNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIiwZ5dKSzVyPU[uNVE1QDdizszN NHT4UotUSU6JRWK=
L-428 NHLYZYFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnvETWM2OD14LkG4NFM4KM7:TR?= NU\2U3k{W0GQR1XS
8-MG-BA NGTk[VJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4rD[2lEPTB;Nj6xPVM4QSEQvF2= NG\uOXBUSU6JRWK=
SK-MEL-28 M17jO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYHlfmh3UUN3ME22MlIxQTZ5IN88US=> NV3JdYxxW0GQR1XS
VA-ES-BJ NVLXSlRST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mnm3TWM2OD14LkOwOFYzKM7:TR?= NYTFVWJwW0GQR1XS
CAL-120 NUjCbY9WT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4PiWmlEPTB;Nj60OVE3OiEQvF2= NYr6[FZMW0GQR1XS
GAMG Mmr4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlHTTWM2OD14LkWwOlIh|ryP MVHTRW5ITVJ?
MSTO-221H Mn33S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEHFVm5KSzVyPU[uOVEyPyEQvF2= MnLEV2FPT0WU
GCT NYqyUFlWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUXONGM3UUN3ME22MlU{Ozh5IN88US=> NEG2VJlUSU6JRWK=
SH-4 NHrIZ|NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1v0dmlEPTB;Nj61PFg1PCEQvF2= MV7TRW5ITVJ?
Calu-3 MnfxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{OzN2lEPTB;Nj62OVU3QCEQvF2= M3zZ[nNCVkeHUh?=
SK-HEP-1 NHjOXo1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHrqZ2pKSzVyPU[uO|E4PTNizszN MYfTRW5ITVJ?
PFSK-1 MoX3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVPJR|UxRTZwN{O0O|gh|ryP NFzEXnBUSU6JRWK=
NB12 M1:zVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIPkSlFKSzVyPU[uO|g1ODRizszN NUDCfWJrW0GQR1XS
A388 MnziS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVjJR|UxRTZwOEm1NkDPxE1? MVrTRW5ITVJ?
KLE MlHkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3PWeGlEPTB;Nj65OlUyPiEQvF2= MmjUV2FPT0WU
TE-11 MkH1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV;qRW5qUUN3ME23MlA1QTZ5IN88US=> NXfQeGFUW0GQR1XS
NCI-H2405 MmG0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{fpS2lEPTB;Nz6wPFM6OyEQvF2= NGfQZXZUSU6JRWK=
OVCAR-4 NXPsZohGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIP0[4ZKSzVyPUeuNVU{PSEQvF2= M1vpXHNCVkeHUh?=
KP-N-YS MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlnYTWM2OD15LkG4NVQh|ryP MoXIV2FPT0WU
NOS-1 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2O3NGlEPTB;Nz6yOVgzQCEQvF2= M2O5OnNCVkeHUh?=
DMS-114 NUC5N5dOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnvLTWM2OD15LkK5OlMyKM7:TR?= M4XwPHNCVkeHUh?=
NCI-H2030 Mn3RS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHXzeHNKSzVyPUeuN|M3QThizszN NUWxfY5jW0GQR1XS
HEL M3PJUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXrJR|UxRTdwM{e1NlEh|ryP MXrTRW5ITVJ?
CAL-62 M4DmVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYHsd5Y1UUN3ME23MlM6QDZ4IN88US=> Mn;DV2FPT0WU
COLO-668 MmrCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmTzTWM2OD15LkSzPVY4KM7:TR?= NHP4U2VUSU6JRWK=
SKG-IIIa MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF\hOWxKSzVyPUeuOFU{QDFizszN MXTTRW5ITVJ?
HCC1419 NG\SOXlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3v3NmlEPTB;Nz60Olg{OyEQvF2= M4DIeXNCVkeHUh?=
OS-RC-2 NUTuXXNTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlHJTWM2OD15LkS5PFk1KM7:TR?= NUOxfI82W0GQR1XS
COLO-829 MlrTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXnJR|UxRTdwNk[0OlMh|ryP NYC4TJR5W0GQR1XS
NB13 M4PKV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MULJR|UxRTdwOEK2Olch|ryP MnvlV2FPT0WU
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EW-22 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWXIXGpSUUN3ME2zNE4yQTZ7IN88US=> M1fUVnNCVkeHUh?=
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C-33-A NYn3SW9ET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYj6OJBxUUN3ME2zOE43QDV5IN88US=> Mnr2V2FPT0WU
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KINGS-1 M2\kNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{nyVmlEPTB;M{WuPFQ{PCEQvF2= NI\xdYFUSU6JRWK=
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NCI-H510A MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIDK[XZKSzVyPUO4MlY1PTFizszN M4TyWnNCVkeHUh?=
NMC-G1 M2GyV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MonmTWM2OD1|OD63NlIzKM7:TR?= M4jYWHNCVkeHUh?=
SW948 M13VdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYn1U3JlUUN3ME2zPE45QTR7IN88US=> NIHCNG1USU6JRWK=
MFM-223 M4K5WWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUXSXlA1UUN3ME2zPE46PDNzIN88US=> MmX4V2FPT0WU
PAN1 NWnvN5oxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYLJR|UxRTN6Lkm5NVkh|ryP NEfpTm1USU6JRWK=
MZ7-mel NWjjNFRtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIfmSGhKSzVyPUO5MlEyQTlizszN Mn3BV2FPT0WU
COLO-679 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn3oTWM2OD1|OT65O|A5KM7:TR?= NY\oTnBuW0GQR1XS
SW872 NHnlcWRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NY[zdXJDUUN3ME20NE4zODNzIN88US=> NGPmZmNUSU6JRWK=
HT-1197 Mli0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mmn1TWM2OD12MD6yPFY6KM7:TR?= MkTxV2FPT0WU
ES6 NFS2XGtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVHJR|UxRTRyLkS1OVIh|ryP NEPSOlNUSU6JRWK=
SNU-387 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVXZRWpOUUN3ME20NE45OjN6IN88US=> MWfTRW5ITVJ?
T47D NVPkdVdDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4HOO2lEPTB;NEGuNFE6OiEQvF2= M3H0W3NCVkeHUh?=
G-402 NGfSUI1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{\2ZWlEPTB;NEGuNFgxQSEQvF2= NEfVUo9USU6JRWK=
MZ1-PC NWi4RpRvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVv1c|dnUUN3ME20NU45OSEQvF2= NF7FU3dUSU6JRWK=
SW1417 NXnYcIRxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmDDTWM2OD12Mj6zNFMh|ryP MmHFV2FPT0WU
RERF-LC-MS Mo\RS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml;yTWM2OD12Mj61NFg4KM7:TR?= NGnneIFUSU6JRWK=
SF539 M3vpOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUS3SIVJUUN3ME20Nk42PTJ7IN88US=> MlnGV2FPT0WU
ESS-1 NIe3O|lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIK3eXdKSzVyPUSzMlU2PThizszN NYPrTVVuW0GQR1XS
RO82-W-1 MlnjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEPNXG1KSzVyPUSzMlYyPTNizszN MmTaV2FPT0WU
S-117 M4Pucmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX7PNnUyUUN3ME20N{43QTV6IN88US=> M4P1[XNCVkeHUh?=
SW756 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4XvN2lEPTB;NEOuPFI1OiEQvF2= MXHTRW5ITVJ?
CP66-MEL NHPReFhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mon2TWM2OD12NT63NVQ{KM7:TR?= MXXTRW5ITVJ?
H-EMC-SS NGqwb|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHvjVnJKSzVyPUS1Mlc3QTNizszN NH3vOFJUSU6JRWK=
NCI-H2052 MlnaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUjJR|UxRTR3LkmwNlch|ryP MknhV2FPT0WU
SW684 NVjs[2tsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVzJR|UxRTR5LkCwPFEh|ryP MXnTRW5ITVJ?
HCC1569 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2SwNWlEPTB;NEeuNVY2QCEQvF2= M4HGWXNCVkeHUh?=
KGN MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHLCdoRKSzVyPUS3MlM{OTFizszN M4rsNHNCVkeHUh?=
LCLC-103H M13BXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn;uTWM2OD12Nz60OFA1KM7:TR?= MlHsV2FPT0WU
SW780 MnnrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUXJR|UxRTR5Lk[zOVEh|ryP NUDY[JdYW0GQR1XS
NCI-H1703 NV7pe5RxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFzRW4RKSzVyPUS4MlM{PTJizszN NWnHcGRxW0GQR1XS
HCC1395 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUDuZppRUUN3ME20PE44PDN{IN88US=> M3nxOXNCVkeHUh?=

... Click to View More Cell Line Experimental Data

In vivo Gefitinib (100 mg/kg) improves the anti-tumor effect of radiotherapy in LoVo tumor xenografts. [3] Gefitinib treatment of nude mice bearing established human GEO colon cancer xenografts reveals a reversible dose-dependent inhibition of tumor growth because GEO tumors resumes the growth rate of controls at the end of the treatment. [4]

Protocol

Cell Research:[1]
+ Expand
  • Cell lines: NR6, NR6M and NR6W cells
  • Concentrations: 0-2 μM
  • Incubation Time: 72 hours
  • Method: Exponentially growing cells including NR6, NR6M, NR6M and NR6W cells are seeded in sextuple in 96-well plates at a concentration of 2000 cells/well, allowed to adhere and subsequently washed in PBS and incubated overnight in medium containing 0.5% FCS. Cells are then treated with varying concentrations (0-2 μM) of Gefitinib or the solute control DMSO and EGF. The optimal EGF concentration for inducing proliferation of NR6wtEGFR and NR6W cells has previously been determined and hence NR6wtEGFR and NR6W cells are supplemented with 10 nM and 0.1 nM EGF, respectively. EGF is not added to NR6 and NR6M cells. After 72 hours the amount of cells are measured by performing a MTT proliferation assay.
    (Only for Reference)
Animal Research:[3]
+ Expand
  • Animal Models: Female nude mice (cba nu/nu) aged 8–10 weeks are intra-dermal injected with LoVo cells.
  • Formulation: 0.5% polysorbate
  • Dosages: 100 mg/kg
  • Administration: Once daily by oral administration (0.1 mL/10 g body weight) for 14 days
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 89 mg/mL (199.14 mM)
Ethanol 4 mg/mL (8.95 mM)
Water <1 mg/mL
In vivo 5% DMSO+corn oil 2.5mg/mL

* 1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 446.90
Formula

C22H24ClFN4O3

CAS No. 184475-35-2
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03050164 Completed Bioequivalence Study Hunan Kelun Pharmaceutical Co., Ltd. September 8, 2016 Phase 1
NCT02856893 Not yet recruiting NSCLC European Organisation for Research and Treatment of Cancer - EORTC|AstraZeneca March 2017 Phase 2
NCT02976116 Recruiting NSCLC Hutchison Medipharma Limited December 2016 Phase 2
NCT02951637 Not yet recruiting Lung Adenocarcinoma Shanghai Chest Hospital|Ruijin Hospital|Changhai Hospital December 2016 Phase 2
NCT02930954 Not yet recruiting Non-small-cell Lung Cancer Caicun Zhou|Tongji University November 2016 Phase 2
NCT02929693 Enrolling by invitation Cancer Shanghai University of Traditional Chinese Medicine|Shanghai Chest Hospital|Shanghai Pulmonary Hospital, Shanghai, China October 2016 Phase 3

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID