Gefitinib (ZD1839)

Catalog No.S1025

Gefitinib (ZD1839) Chemical Structure

Molecular Weight(MW): 446.90

Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.

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Cited by 93 Publications

14 Customer Reviews

  • c, HCC4006 lung cancer cells that have acquired a high-mesenchymal state concomitant with becoming resistant to gefitinib (red curve) exhibit increased sensitivity to GPX4 inhibition.

    Nature, 2017, 547(7664):453-457. Gefitinib (ZD1839) purchased from Selleck.

    Perturbation of EGFR by its ligand EGF and gefitinib (ZD-1839 Iressat; inhibits EGFR) produces opposite responses in the predicted EGFR target genes SOCS2 and NR2E1.

    Mol Syst Biol 2011 7, 486. Gefitinib (ZD1839) purchased from Selleck.

  • Effects of shRNA-mediated RECK depletion on EGFR signaling in MEFs. (a) Immunoblotting (IB) of the indicated proteins in wild-type MEFs transduced with the indicated shRNAs and cultured for 48 h in the presence of 0.1% dimethyl sulfoxide (DMSO; vehicle) or 1 mM gefitinib (S1025, Selleck Chemicals,Houston, TX, USA). (b) Immunoprecipitation from whole lysates of cells from panel a with an anti-phosphorylated tyrosine (pTyr) antibody. Precipitates from 500 mg protein in whole cell lysates (upper) or 20 mg protein in whole cell lysates (lower) were analyzed by IB with anti-EGFR antibody.

    Oncogene 2010 30, 737-750. Gefitinib (ZD1839) purchased from Selleck.

    (B–C) LNCaP (B) and LNCaP-AI (C) cells were transiently transfected with sPLA2-IIa(-800)-Luc (0.5 μg). The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 lM) and Bortezomib (20 μM) without or with EGF (100 ng/ml) for 24 h. Luciferase assay was performed according to a standard protocol with Renilla luciferase as an internal control. Data are presented as the mean (±SD) of duplicate values of a representative experiment that was independently repeated for five times.

    Carcinogenesis 2010 31, 1948–1955 . Gefitinib (ZD1839) purchased from Selleck.

  • LNCaP-AI cells were starved in 1% stripped medium for 24 h. The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 μM) and Bortezomib (20 μM) for 24 h. Cell culture medium was collected from each sample and subjected to ELISA for sPLA2-IIa. The condition medium samples were diluted 10 times for ELISA. Average of duplicate samples was converted to nanogram per milliliter against standard curve. The data represent one of five repeated experiments.

     

     

    Carcinogenesis 2010 31, 1948–1955. Gefitinib (ZD1839) purchased from Selleck.

    Susceptibility of lung cancer cells to cytolytic activity of NK-92 cells after treatment with EGFR inhibitors. Three lung cancer cells were untreated (open circle) or treated with 10 μM erlotinib or gefitinib (black filled square or black filled triangle) for 24 hours. The lung cancer cells were cocultured with NK-92 cells at indicated effector to target ratio (E:T ratio). To determine the specificity of NKG2D-mediated cytolysis of lung cancer cells, NK-92 cells were preincubated with blocking mAb against NKG2D before the assay (gray filled square and triangle) and correspond-ing isotype mAb (open square and triangle).

    J Immunother 2011 34(4), 372-81. Gefitinib (ZD1839) purchased from Selleck.

  • Suppression of EGFR signaling by Gefitinib is dose dependent and non- toxic at millimolar concentrations.Results from plaque reduction tests of Hep2 cells infected with VACV(12.5pfu/well) treated with a serial dilution of Gefitinib (1000-0.01 m) and analyzed for (A)proliferation as indicator of cytotoxicity,(B) IC50 of plaque size inhibition, (C)dose dependent inhibition of EGFR-ERK1/2signaling and VACV proteins by Western blot analysis (pEGFR detection was obtained from a different experiment with identicals ettings) and (D)dose dependent inhibition of orthopoxvirus genome replication by real-time PCR.Untreated VACV infectedcells and uninfected cells are shown as controls in the right panel.

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

    A circular zone of EGFR activated but uninfected cells facilitate virus induced plaque development , efficiently blocked by Gefitinib. (A)Immun of luorescent staining of aplaque reduction tests with Hep2 cells 24,48,72 and 96 h after VACV infection (6.25pfu/chamber). VACV infected cells(green) were visualized by human IgG directed to orthopoxviruses (VIG) detected with a secondary FITC labelled antibody. Uninfected cells with activated EGFR signaling(red) are detected with a secondary Cy3labelled antibody. Positive cells for both , VACV and phosphorylated EGFR are shown in yellow(merge). Cellular nuclei are counterstained with DAPI. (B)Immunofluorescent staining of a plaque reduction test as described for (A) and treated with three different Gefitinib concentrations (1000 nM, 1 nM, and 0.01 nM) . The test was analyzed 96h afterVACV infection.Uninfected cells are shown as controls. Magnification100×.(For interpretation of the references to color in this figure legend , the reader is referred to the web version of the article.)

     

     

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

  • The EGF-dependent increased association of PCM1 and AZI1 requires EGFR kinase activity. ZD1839 (Iressa)—EGFR tyrosine kinase inhibitor.

    Mol Biosyst 2011 7, 3223-33. Gefitinib (ZD1839) purchased from Selleck.

    Inhibition of 5-HT-induced 5-HT3A and 5-HT3AB receptor currents.

    Eur J Pharmacol, 2017, 821:21-28. Gefitinib (ZD1839) purchased from Selleck.

  • Reduction of GPER expression in HCC1806 cells after treatment with gefitinib. (A) Concentration dependence. HCC1806 cells were treated with 50-500 nM gefitinib for 48 h. GPER was detected by western blotting. (B) Time dependence. HCC1806 cells were treated with 200 nM gefitinib for 24-96 h. ap<0.05 vs. control; bp<0.001 vs. control. Mean values and SE of three independent experiments are shown. (C) Representative western blotting of concentration dependence. (D) Representative western blotting of time dependence

    Oncol Rep, 2017, 37(2):1212-1218. Gefitinib (ZD1839) purchased from Selleck.

    Inhibition of signaling pathway activation in lung tumor cell lines by kinase inhibitors. Lung tumor cells were cultured in 10% FBS until reaching ∼80% confluence and then the cells were starved in serum-free medium for overnight, followed by 4-hour treatment with the inhibitors. Cell lysates were then prepared and used for determination of the pathway activation signals by the CEER assay.

    Int J Proteomics 2011 215496. Gefitinib (ZD1839) purchased from Selleck.

  • Breast cancer cells were pretreated with 100ng/ml EGF for 15 min and then treated with the indicated concentrations of  Gefitinib for 24 hours.

     

     

    2010 Dr. Zhang of Tianjin Medical University. Gefitinib (ZD1839) purchased from Selleck.

    MTT assay result. 0-500nM Gefitinib treated with H1299, H358, H25 and HCC827 cell lines by 72h, Proliferation rate was tested.

     

     

    2010 Dr. Vicky Tin from University of Hong Kong. Gefitinib (ZD1839) purchased from Selleck.

Purity & Quality Control

Choose Selective EGFR Inhibitors

Biological Activity

Description Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.
Features A potent EGFR tyrosine kinase inhibitor.
Targets
Tyr1173 (NR6W cells) [1] Tyr1173 (NR6wtEGFR cells) [1] Tyr992 (NR6wtEGFR cells) [1] Tyr992 (NR6W cells) [1]
26 nM 37 nM 37 nM 57 nM
In vitro

Gefitinib effectively inhibits all tyrosine phosphorylation sites on EGFR in both the high and low-EGFR-expressing cell lines including NR6, NR6M and NR6W cell lines. The phosphorylation sites Tyr1173 and Tyr992 are less sensitive requiring higher concentrations of Gefitinib for inhibition. Gefitinib effectively blocks the phosphorylation of PLC-γ, with IC50 of 27nM, in NR6W cells. The NR6wtEGFR and NR6M cell lines has low levels of PLC-γ phosphorylation but the level in the NR6M cell line is more resistant to inhibition by Gefitinib with IC50 of 43 nM and 369 nM, respectively. Gefitinib inhibits Akt phosphorylations, with IC50 of 220 and 263nM, in the low-EGFR- and -EGFRvIII-expressing cell lines, respectively. Gefitinib in the dose range from 0.1 to 0.5μM significantly facilitates, rather than abrogates, colony formation of NR6M cells. However, at a concentration of 2 μM Gefitinib completely blocks NR6M colony formation. Gefitinib rapidly and in a dose-dependent manner inhibits EGFR and ERK phosphorylation up to 72 hours after EGF stimulation in both the high- and low-EGFR-expressing cell lines. [1] Gefitinib is the monolayer growth of these EGF-driven untransformed MCF10A cells with an IC50 of 20 nM. [2] The combination of Gefitinib (0.2 μM and 0.5 μM) with irradiation lead to a significant growth inhibition in LoVo cells, compared with radiation alone.. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KB M1XGOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoTzTWM2OD1yLkC1OEDPxE1? NGLsVWwyPjVzNkS3Ny=>
NIH3T3 NXn6Zmo5TnWwY4Tpc44hSXO|YYm= M4HNUVAvOiEQvF2= NIfnXFEzKGh? Mn7TSG1UVw>? MYfJcohq[mm2aX;uJI9nKEWJRjDzeIlufWyjdHXkJIh2dWGwIHXyZmIyKGG3dH;wbI9{eGixconsZZRqd25id3n0bEBKSzVyIH;mJFAvODF2NDFOwG0> MmLqNVY1QDB{OES=
COS7 MVLGeY5kfGmxbjDBd5NigQ>? M3;ZOFk3KM7:TR?= MUnJcohq[mm2aX;uJI9nKGi3bXHuJGpCUzJiVk[xO2YhdXW2YX70JIV5eHKnc4Pl[C=> MonINVcyPzh5MkK=
A549 NHnrcYZEgXSxdH;4bYMhSXO|YYm= M1L2flk3KGh? MoWySG1UVw>? NUfUZpllUUN3ME2xN{42QSEQvF2= MYmxPFMyOzhyNx?=
K562 NETaSldEgXSxdH;4bYMhSXO|YYm= NFvSS|Y6PiCq NIDaR5RFVVOR MljCTWM2OD17LkO2JO69VQ>? M2rIb|E5OzF|OEC3
MDA-MB-231 MXTDfZRwfG:6aXOgRZN{[Xl? M4rEVlk3KGh? NWn2dJNuTE2VTx?= MkPwTWM2OD1|Nj62NUDPxE1? MoDDNVg{OTN6MEe=
MOLT4 M3fSemN6fG:2b4jpZ{BCe3OjeR?= NIfxV|Y6PiCq NGrJeYRFVVOR MoPYTWM2OD1zNT6wNkDPxE1? M3\Y[FE5OzF|OEC3
MCF7 MnrXR5l1d3SxeHnjJGF{e2G7 NYPt[o1tPTBizszN NIPle4I4OiCq MUHJR|UxRTF{LkC1JO69VQ>? MoSxNVg4PzF6MUm=
BT474 NYrwVZpNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUTUT2dGPzJiaB?= MkDPRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCEVES3OEBk\WyuczDveoVz\XiycnXzd4lv\yCHUlLiNkB4cXSqIFXDOVAhd2ZiMD6zJO69VQ>? NWXPNGtJOTlyMki0NlQ>
HN5 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4LPOFczKGh? NVTORW1pSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIUlUh[2WubIOgc5ZmemW6cILld5NqdmdiRVfGVkB4cXSqIFXDOVAhd2ZiMD6wNUDPxE1? NFzmOpAyQTB{OESyOC=>
BA/F3 M4XWOmN6fG:2b4jpZ{BCe3OjeR?= MnrCO|IhcA>? MXrDfZRwfG:6aXPpeJkh[WejaX7zeEBud3W|ZTDCRU9HOyClZXzsd{BmgHC{ZYPzbY5oKEWJRmKgUFg2QFJibYX0ZY51KHerdHigTWM2OCCxZjCwMlAyODhizszN NUPKfYFzOTl{M{myNlk>
NIH3T3 NX;ae29bT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH6wbnk4OiCq MWXHdo94fGhiaX7obYJqfGmxbjDv[kBud3W|ZTDOTWgwO1R|IHPlcIx{KGW6cILld5NqdmdiZYLCNkBo\W6nIIfpeIghUUN3MDDv[kAxNjV2IN88US=> NWX0XXVXOTl4NkWzO|c>
MCF7 MX NGD2XFdHfW6ldHnvckBCe3OjeR?= MW[zNEBucW5? M3H3VWlvcGmkaYTpc44hd2ZiQlPSVEBmgHC{ZYPz[YQhf2m2aDDJR|UxKG:oIEGuNUDPxE1? Ml7UNVk6OzJ7NkC=
A431 Mn\OS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGPQcWkyOCEQvF2= NELh[WI4OiCq MUHHTVUxRTBwNUG0JO69VQ>? MlHLNVk6Pjl2NkW=
A431 MX\LbY5ie2ViQYPzZZk> NVPoR5NTOC5zIN88US=> NWXMcWRGOSCq MVnJcohq[mm2aX;uJI9nKEWJRj3pcoR2[2WmIFXHSnIheGixc4Doc5J6dGG2aX;u NUnlRZhCOTl7Nkm0OlU>
A431 NUjaVnU{U2mwYYPlJGF{e2G7 Mn;DNVAxKM7:TR?= NYTZWYY{OTBibXnu M2nHUXBzd2yxbnfl[EBqdmirYnn0c5J6KGGldHn2bZR6KG:oIFXHSk1qdmS3Y3XkJGVITlJicHjvd5Bpd3K7bHH0bY9v NVrMdoYxOTl7Nkm0OlU>
Sf9 MkCyT4lv[XOnIFHzd4F6 MUOxNEBucW5? NUfHVGppTE2VTx?= NEPB[mdKdmirYnn0bY9vKG:oIFXHSnIh\XiycnXzd4VlKGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhemWlZYD0c5Ih[XW2b4Doc5NxcG:{eXzheIlwdiC5aYToJGlEPTBib3[gNE4xOzNizszN MkHINlAxPTZ2MkW=
NCI-H1975 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHe1XGM2KM7:TR?= NV\5d4RXPzJiaB?= MUfJR|UxRThwMk[g{txO MoPPNlAyPTF4N{C=
NCI-H1975 MoDaT4lv[XOnIFHzd4F6 MYixNEDPxE1? NF;KSYpKdmirYnn0bY9vKG:oIFXHSnIh[XW2b4Doc5NxcG:{eXzheIlwdg>? M{Ww[|IxOTVzNkew
NCI-H1975 MYDGeY5kfGmxbjDBd5NigQ>? M1XQTlExKM7:TR?= MWLJcohq[mm2aX;uJI9nKEWUQlKyJIF2fG:yaH;zdIhwenmuYYTpc44> MXqyNFE2OTZ5MB?=
NCI-H1975 NHzQWFlHfW6ldHnvckBCe3OjeR?= NVrhUXhYPSEQvF2= MXq0PEBp MoDFTY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCjc4Pld5Nm\CCjczDhZ5RqfmG2aX;uJI9nKGOjc4Dhd4UuOw>? M2LSXlIxOTVzNkew
A375 M2TjTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYLMRWU4PDhiaB?= NEnifHlKSzVyPUKwMlU2KM7:TR?= MWqyNFMxPDV|Nx?=
SMMC7721 MmfzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX:0PEBp NIDXNGxKSzVyPUOzMlEzKM7:TR?= NH3IRVIzODNyNEWzOy=>
DU145 Moi0R5l1d3SxeHnjJGF{e2G7 NF[5T202KGR? NX3KOXo3TE2VTx?= NViyc4RTUUN3ME20Nk4yPyEQvF2= NYfjdGF3OjB2Nk[1OVU>
MIAPaCa2 MnfwR5l1d3SxeHnjJGF{e2G7 NGjzeJA2KGR? MnTwSG1UVw>? M3HQPWlEPTB;NUCuOlIh|ryP M37yblIxPDZ4NUW1
PANC1 M2DGU2N6fG:2b4jpZ{BCe3OjeR?= NHPFVWs2KGR? NULLVYwxTE2VTx?= MVTJR|UxRTRyLkSyJO69VQ>? MVOyNFQ3PjV3NR?=
PC9 NWruUVl5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmfyRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDFS2ZTNWSnZnnjbYVvfCCqdX3hckBRSzliY3XscJMhf2m2aDDFR|UxKG:oIECuNFE4KM7:TR?= NHzmUHQzOTJyOEiwNi=>
PC9 NGK1NYxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlWxRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDndo94fGhvcnXzbZN1[W62IHj1cYFvKFCFOTDj[YxteyCneIDy[ZN{cW6pIFXHSnIhTTd2Nm;BO|UxN1R5OUDNJI12fGGwdDD3bZRpKEWFNUCgc4YhPyEQvF2= NX;6T|hFOjF{MEi4NFI>
PC3 M4rK[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYixNEDPxE1? NGTRW|g4OiCq MoPGSG1UVw>? M1G4NWlEPTB;Nz60JO69VQ>? MmjRNlE{PTN3NE[=
MDA-MB-468 NX21PWlOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{G2eVExOCEQvF2= MV:0PEBp NHfBe45FVVOR Mlf5RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdIm= MWeyNVQzQTZ|Mh?=
Plasmodium falciparum MUnxTHRUKHC{b3\pcIlv\yCob4KgbY5pcWKrdH;yd{Bw\iCSbHHzcY9lcXWvIH\hcINqeGG{dX2gdJJwdGmoZYLheIlwdg>? NUK5OHZ6OjF6MUewOFU>
NCI-H1993 NG\h[olIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml7mO|IhcA>? M4fIeGROW09? NXu1dpFnUUN3ME2wMlEh|ryP M33QXVIyQTF4NEOz
NCI-H292 NEDIeVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnX1O|IhcA>? NIDBeWtFVVOR NViySJoxUUN3ME2wMlAzKM7:TR?= NVTORVJYOjF7MU[0N|M>
A431 M4DE[2tqdmG|ZTDBd5NigQ>? NEXYWJYzKGh? NYfQNJd1TE2VTx?= MUjJcohq[mm2aX;uJI9nKEWJRmKgdIhwe3Cqb4L5cIF1cW:wIHnuJGVITi2|dHnteYxifGWmIHj1cYFvKEF2M{Gge4l1cCCLQ{WwJI9nKDBwMECxJO69VQ>? NIf4UXozOjFyMUGzNi=>
MCF7 MoHZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUL2cWU1OTBywrFOwIcwdUx? Mlf5OFghcA>? MU\EUXNQ NEnkWYtCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgc5ZmemW6cILld5NqdmdiRVfGVkBo\W6nIIfpeIghUUN3MDDv[kAyOi5yNTFOwG0> NIP4U|gzOjFzOUGzNC=>
BT474 MXTDfZRwfG:6aXOgRZN{[Xl? MV[xNEDPxE1? NHKwOIo1QCCq NVLkd2lxS3m2b4TvfIlkcXS7IHHnZYlve3Ric3XyeY0ue3Sjco\l[EBpfW2jbjDCWFQ4PCClZXzsdy=> MWCyNlE{QDNyOB?=
MCF7 NWTrSFd6S3m2b4TvfIlkKEG|c3H5 MVKxNEDPxE1? M2SxcFQ5KGh? M1PNTGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KHOncoXtMZN1[XK4ZXSgbJVu[W5iTVPGO{Bk\WyuczDveoVzNWW6cILld5NqdmdiRYLiRlIh[2WubIO= NUTORnpxOjJzM{izNFg>
HCC827 NUXEZYpmU2mwYYPlJGF{e2G7 MXuwMlEzPSEQvF2= MoHMNlQhcA>? M1LOdGROW09? M4X5[WlvcGmkaYTpc44hd2ZiRVfGVkBl\WxiRUe0Ok1CPzVyIH31eIFvfCC\MUC2PEBifXSxcHjvd5Bpd3K7bHH0bY9v MYqyNlM{QTN2Mh?=
HCC827 M1nIVmtqdmG|ZTDBd5NigQ>? MWqwMlEzPSEQvF2= MUiyOEBp M{T2VmROW09? MV\Jcohq[mm2aX;uJI9nKEWJRmKg[IVtKEV5NE[tRVc2OCCvdYThcpQudWWmaXH0[YQhTXKtIIDoc5NxcG:{eXzheIlwdg>? MYCyNlM{QTN2Mh?=
HCC827 MWTLbY5ie2ViQYPzZZk> NYfYc|JsOC5zMkWg{txO Ml7sNlQhcA>? NFfLUY1FVVOR NYHUNGdFUW6qaXLpeIlwdiCxZjDFS2ZTKGSnbDDFO|Q3NUF5NUCgcZV1[W62LX3l[IlifGWmIFHreEBxcG:|cHjvdplt[XSrb36= M{KwdFIzOzN7M{Sy
A431 MWjLbY5ie2ViQYPzZZk> NYXiSIE4OTByIN88US=> MUezNEBucW5? NYCx[XFLUW6qaXLpeIlwdiCxZjDFS2ZTKGSncnn2[YQh\nKxbTDoeY1idiCDNEOxJINmdGxibXXtZpJidmViYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCyb3z5MWdtfS2WeYKgdIhwe3Cqb4L5cIF1cW:wIIfpeIghUUN3MDDv[kAyNjB6IN88US=> NUDYW4d7OjJ4OUi3PFI>
H460 MnTyR5l1d3SxeHnjJGF{e2G7 NVrG[GVzPzJiaB?= MmKyTWM2OD13LkW5JO69VQ>? MlztNlI4Ojd2NEm=
HT-29 MWXDfZRwfG:6aXOgRZN{[Xl? NHHuO3U4OiCq MXLJR|UxRTNwM{[g{txO NHu3clYzOjd{N{S0PS=>
SGC7901 M370WGN6fG:2b4jpZ{BCe3OjeR?= MnOyO|IhcA>? MUfJR|UxRTFyLkK2JO69VQ>? NVqwPZFWOjJ5Mke0OFk>
A498 MnfpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3v3cWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUS5PEBk\WyuczDlfJBz\XO|aX7nJGVITlJiYX7kJHNTSyC5aYToJGlEPTBib3[gPE43KM7:TR?= NUfCVmNLOjJ6MUi4OFg>
Calu3 NUn5WJVUS3m2b4TvfIlkKEG|c3H5 NFrtVoIyOCEQvHevcWw> NFThcFQ4OiCq MkKyTWM2OD1zLk[g{txO MUmyN|EyPjF4OB?=
FADU Mk\NR5l1d3SxeHnjJGF{e2G7 MUWxNEDPxGdxbVy= NYPqZpBYPzJiaB?= NF\6WHlKSzVyPUCuNFUh|ryP M4fa[VI{OTF4MU[4
HCC827 MUnDfZRwfG:6aXOgRZN{[Xl? NWnhe49zOTBizsznM41N MYm3NkBp NYL4[WZEUUN3ME2wMlAxOTVizszN MYiyN|EyPjF4OB?=
PC9 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1vi[VExKM7:Zz;tUC=> MkTtO|IhcA>? NWHWRYlSUUN3ME2wMlAxOTRizszN NFXIXJYzOzFzNkG2PC=>
Sf9 NGHYdotMcW6jc3WgRZN{[Xl? M1TRVVEhcA>? MlHSSG1UVw>? NEDwSVJKdmirYnn0bY9vKG:oIHj1cYFvKEeVVD30ZYdo\WRiRVfGVkBNQDN2UjDteZRidnRiZYjwdoV{e2WmIIfpeIghUUN3MDDv[kAxNjBzNTFOwG0> MnrXNlM3OTF4OUG=
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IGROV-1 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUXJR|UxRTJwM{GyN|ch|ryP M1G5dnNCVkeHUh?=
JVM-3 NVTzZ5Z[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUTJR|UxRTJwM{S2OFMh|ryP NHzabGJUSU6JRWK=
NCI-H2170 NIDhcIJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUXJR|UxRTJwM{S3PFMh|ryP NV\T[4dMW0GQR1XS
EW-18 MmLMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{LCNWlEPTB;Mj6zOVQ4OSEQvF2= NHTqOJdUSU6JRWK=
KU812 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1K5OGlEPTB;Mj6zOlc4QSEQvF2= NIXLc4JUSU6JRWK=
NY M{PTTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWnJR|UxRTJwM{eyO{DPxE1? NFPa[GhUSU6JRWK=
HTC-C3 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV7venlvUUN3ME2yMlQzOjZ5IN88US=> MVHTRW5ITVJ?
CFPAC-1 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIjK[4lKSzVyPUKuOFM5PjRizszN NULrR3MyW0GQR1XS
HH MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVvJR|UxRTJwNEm4OVQh|ryP NIfaUIlUSU6JRWK=
MKN7 NEPydXZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHyyb4lKSzVyPUKuOlI4PDhizszN NW\KZYM4W0GQR1XS
TE-8 MkfPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYrYbVJ3UUN3ME2yMlY{PjV|IN88US=> NHm0U|hUSU6JRWK=
MC-IXC NILtbmZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3HYcWlEPTB;Mj62OFQ2OyEQvF2= M2HRU3NCVkeHUh?=
DMS-79 NHnyb5BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFzucZhKSzVyPUKuOlkzQDRizszN M{nKSnNCVkeHUh?=
Detroit562 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYXTO5RYUUN3ME2yMlY6QDR3IN88US=> M3jvU3NCVkeHUh?=
HuP-T4 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXjJR|UxRTJwN{K4NVgh|ryP M4m4cXNCVkeHUh?=
QIMR-WIL NVe2Wo1VT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXfJR|UxRTJwN{W5OVIh|ryP MkG2V2FPT0WU
BB65-RCC M3fVfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MljVTWM2OD1{LkiwO|E{KM7:TR?= M2TEWHNCVkeHUh?=
PC-3 M3PmO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnjKTWM2OD1{LkizN|E4KM7:TR?= Mn3MV2FPT0WU
OVCAR-3 MlflS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mo\wTWM2OD1{Lki1OVk1KM7:TR?= NEn5OHpUSU6JRWK=
KYSE-510 M2LRPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXjUbW1XUUN3ME2yMlg3Pzh4IN88US=> M4\PVnNCVkeHUh?=
HC-1 M2HsUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVLJXGpzUUN3ME2yMlk1ODF{IN88US=> MlTHV2FPT0WU
NCI-H1693 M4D3dGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn7lTWM2OD1{Lkm1PFk4KM7:TR?= NH7MNIxUSU6JRWK=
DU-4475 NXHSc5JGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVPJR|UxRTJwOUmwOVIh|ryP MW\TRW5ITVJ?
COLO-680N NXrweG9YT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmDFTWM2OD1|LkCwOFk2KM7:TR?= MoTJV2FPT0WU
MN-60 NWnSZ|lbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYLXPHNXUUN3ME2zMlA1QTd2IN88US=> MVrTRW5ITVJ?
BCPAP NXTDeFNWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn64TWM2OD1|LkC4Nlk2KM7:TR?= MV\TRW5ITVJ?
647-V M3\WWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmnhTWM2OD1|LkG2NlIh|ryP NWrJfHlOW0GQR1XS
HT-1376 MnTaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXvJR|UxRTNwMkC1NFYh|ryP MmnCV2FPT0WU
NCI-H1793 M1vMVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn7rTWM2OD1|LkKxNFk5KM7:TR?= M3[4W3NCVkeHUh?=
SW1463 NYXsRnFpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWDsfGZRUUN3ME2zMlI3OTl{IN88US=> NIjxSGJUSU6JRWK=
NCI-H727 MlTDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYXJR|UxRTNwM{i1PFQh|ryP M3Xx[nNCVkeHUh?=
LB771-HNC M4LtTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NY\qemZTUUN3ME2zMlM6OTJ4IN88US=> NGrONJpUSU6JRWK=
D-542MG MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{HINWlEPTB;Mz60OFIzOiEQvF2= MULTRW5ITVJ?
NTERA-S-cl-D1 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUftU25MUUN3ME2zMlQ1QTV5IN88US=> MW\TRW5ITVJ?
C8166 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYq0WWZOUUN3ME2zMlQ3PTJzIN88US=> M4D1THNCVkeHUh?=
639-V Mn\MS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXvJR|UxRTNwNUe3PVIh|ryP NYfSN4N5W0GQR1XS
TE-1 NETBUJdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGfXN21KSzVyPUOuOVkyPjlizszN NE\vPHNUSU6JRWK=
OAW-28 NXPjRodNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF\yZnpKSzVyPUOuOVk1OjZizszN MnXWV2FPT0WU
SK-LU-1 NVTGc5ZRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NICwOVlKSzVyPUOuOlA3PDVizszN M1ryVXNCVkeHUh?=
CaR-1 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHW1RZhKSzVyPUOuOlExPDhizszN Mom3V2FPT0WU
A2780 M1SyVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH\WbYtKSzVyPUOuOlQxQDlizszN Mo\6V2FPT0WU
C2BBe1 NILTOW5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXOyVHE{UUN3ME2zMlY1PDB5IN88US=> NGPmRWRUSU6JRWK=
SK-MES-1 M4C4UGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4LnO2lEPTB;Mz62OFYyPCEQvF2= NH7COXpUSU6JRWK=
PANC-10-05 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYLSSlJHUUN3ME2zMlczODd{IN88US=> MVvTRW5ITVJ?
J-RT3-T3-5 NYTuT2lGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXTJR|UxRTNwN{W0NFYh|ryP Mln1V2FPT0WU
ES4 MoHsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVH4e5NSUUN3ME2zMlgzOjh4IN88US=> MWrTRW5ITVJ?
NCI-H28 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2XkNmlEPTB;Mz64Nlk3OSEQvF2= M{L4ZXNCVkeHUh?=
NCI-H1155 M{PWSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGj6XmxKSzVyPUOuPFQyPTlizszN M{f3e3NCVkeHUh?=
KURAMOCHI NYniUXVzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUfJR|UxRTNwOE[wOVYh|ryP NHLWboRUSU6JRWK=
NCI-H69 NY\3fo5oT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWrJR|UxRTNwOUCxO|gh|ryP MmqxV2FPT0WU
IST-MES1 NIH2UY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{HKSmlEPTB;Mz65OFAzQSEQvF2= NHroSm9USU6JRWK=
NH-12 NVny[ZFCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4DBd2lEPTB;Mz65OFE5PyEQvF2= MmPsV2FPT0WU
JVM-2 Mmm4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1SwNGlEPTB;Mz65OFc2OiEQvF2= M2C2XHNCVkeHUh?=
EW-16 NUfDZ2ZUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoP1TWM2OD1|Lkm1OVc4KM7:TR?= NYrvXmhTW0GQR1XS
NCI-H441 NYq1cpd{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXjJR|UxRTNwOUewNVQh|ryP NEnrdFJUSU6JRWK=
RCC10RGB NEDmUJRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2i2dmlEPTB;ND6wNVI1PiEQvF2= M1TE[3NCVkeHUh?=
TCCSUP MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYLJR|UxRTRwMUGwOUDPxE1? M17SS3NCVkeHUh?=
HL-60 NIP1c4VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2fnd2lEPTB;ND6xNlI1OSEQvF2= M3X1O3NCVkeHUh?=
EW-11 NWPpb2tiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWHJR|UxRTRwMUOyOlEh|ryP M{LKUnNCVkeHUh?=
KARPAS-299 M1z5WWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHLlWnBKSzVyPUSuN|ExOTNizszN NEDlWYtUSU6JRWK=
SNU-C2B NYG4RXZlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWXJR|UxRTRwM{S2PFYh|ryP MUDTRW5ITVJ?
LU-139 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX;JR|UxRTRwM{eyOFch|ryP M4XFfnNCVkeHUh?=
HCC1937 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEX5W2NKSzVyPUSuN|c6QTNizszN NFfPOlFUSU6JRWK=
GR-ST MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkfRTWM2OD12LkSzNVgh|ryP MVPTRW5ITVJ?
SK-OV-3 M1HZWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGfZV4pKSzVyPUSuOFYxPDJizszN MWLTRW5ITVJ?
A172 M{LRV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYD0TmNGUUN3ME20MlU2PTJzIN88US=> M{\v[XNCVkeHUh?=
NB10 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWfJR|UxRTRwNUm3PFEh|ryP NVez[ZJEW0GQR1XS
P30-OHK MoWyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUfJR|UxRTRwOEG5Nlkh|ryP M4XK[nNCVkeHUh?=
KYSE-520 MlvUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFHpfGZKSzVyPUSuPFgxOThizszN MUfTRW5ITVJ?
KNS-62 MonMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIXGNIhKSzVyPUSuPVA5ODdizszN MXPTRW5ITVJ?
ES1 Mn7wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MU\JR|UxRTRwOU[wN|Uh|ryP NF\0RpVUSU6JRWK=
KOSC-2 M3v1OGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFj1VFlKSzVyPUSuPVk6PTlizszN MonlV2FPT0WU
TE-9 M1y4VWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV;JR|UxRTVwMEG1NlUh|ryP MXnTRW5ITVJ?
NCI-H1838 MmHVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVfRPWpsUUN3ME21MlAzODd4IN88US=> MXXTRW5ITVJ?
KP-N-YN NUnrfoMxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUfJR|UxRTVwMUK1PVEh|ryP M2rBOHNCVkeHUh?=
ALL-PO MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnzaTWM2OD13LkKyOVg4KM7:TR?= MojXV2FPT0WU
NOMO-1 NGGwS|NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnvKTWM2OD13LkKzNVc3KM7:TR?= MUPTRW5ITVJ?
CGTH-W-1 NX3YU3N4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlLkTWM2OD13LkO4OVg1KM7:TR?= MX;TRW5ITVJ?
ATN-1 NVHneldiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXTDZ3JoUUN3ME21MlQyOTh2IN88US=> MYrTRW5ITVJ?
AGS MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH;DRo9KSzVyPUWuOFk3OThizszN Mmr3V2FPT0WU
SW13 M3zW[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkDCTWM2OD13LkWzNlY2KM7:TR?= MXTTRW5ITVJ?
SW1990 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX;JR|UxRTVwNkKyPFUh|ryP M3LSeHNCVkeHUh?=
BFTC-905 MoPJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWf0T4kzUUN3ME21MlY2QDd6IN88US=> NIDo[3lUSU6JRWK=
RMG-I NIrKfopIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmW0TWM2OD13Lk[1PVQzKM7:TR?= NYTV[2pZW0GQR1XS
HD-MY-Z NWnvS|RTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGX2OXFKSzVyPUWuOlg2QDFizszN NUHWUJB[W0GQR1XS
HCC1187 MkTuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXnXZoZ{UUN3ME21MlcyODN{IN88US=> NI\ES5lUSU6JRWK=
HCC2998 Ml;MS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NG\ONZdKSzVyPUWuO|E5PzdizszN MX3TRW5ITVJ?
RT-112 M1joOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NETneYpKSzVyPUWuPVAzPDVizszN MmfuV2FPT0WU
CAKI-1 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3fwbmlEPTB;Nj6wNFk5PSEQvF2= NY\2TpNiW0GQR1XS
HDLM-2 MnzmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXvJR|UxRTZwMEG0PFkh|ryP M4TPT3NCVkeHUh?=
TE-10 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmLyTWM2OD14LkC0PFM{KM7:TR?= NXjW[GxyW0GQR1XS
KE-37 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4XGSGlEPTB;Nj6wO|I2QCEQvF2= MnW2V2FPT0WU
RCM-1 NVPF[ppMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXj6c5ljUUN3ME22MlA4PzF7IN88US=> MUfTRW5ITVJ?
Mewo NVrnZ3B7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYTJR|UxRTZwMUG0PFch|ryP MlWxV2FPT0WU
L-428 MmPmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnfPTWM2OD14LkG4NFM4KM7:TR?= MVzTRW5ITVJ?
8-MG-BA MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVLJR|UxRTZwMUmzO|kh|ryP NXnIfnpbW0GQR1XS
SK-MEL-28 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX3JR|UxRTZwMkC5Olch|ryP MkLwV2FPT0WU
VA-ES-BJ Mlf1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXzJR|UxRTZwM{C0OlIh|ryP MXjTRW5ITVJ?
CAL-120 NFjmZXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1T3PWlEPTB;Nj60OVE3OiEQvF2= MoDpV2FPT0WU
GAMG M3;3OWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYTJR|UxRTZwNUC2NkDPxE1? MYHTRW5ITVJ?
MSTO-221H NHHZRphIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4rwfmlEPTB;Nj61NVE4KM7:TR?= NETRR5NUSU6JRWK=
GCT MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFXvfFZKSzVyPU[uOVM{QDdizszN M3vEZXNCVkeHUh?=
SH-4 NVfrXXlrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MknITWM2OD14LkW4PFQ1KM7:TR?= M1KzPXNCVkeHUh?=
Calu-3 MkDsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGDhZmJKSzVyPU[uOlU2PjhizszN MVLTRW5ITVJ?
SK-HEP-1 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4q2NmlEPTB;Nj63NVc2OyEQvF2= NHO3cYVUSU6JRWK=
PFSK-1 NWOxPVE5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmniTWM2OD14LkezOFc5KM7:TR?= MU\TRW5ITVJ?
NB12 NXnmfndXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3XS[2lEPTB;Nj63PFQxPCEQvF2= M4DQfHNCVkeHUh?=
A388 NGHYNW5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1PQNGlEPTB;Nj64PVUzKM7:TR?= NX\wNmJPW0GQR1XS
KLE NXLxVG1LT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVHJR|UxRTZwOU[1NVYh|ryP Ml;lV2FPT0WU
TE-11 M{\H[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnjDTWM2OD15LkC0PVY4KM7:TR?= NID4Sm5USU6JRWK=
NCI-H2405 NWr6RVk1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M37JfGlEPTB;Nz6wPFM6OyEQvF2= MlHQV2FPT0WU
OVCAR-4 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHi3ZnFKSzVyPUeuNVU{PSEQvF2= MVLTRW5ITVJ?
KP-N-YS NHLjT4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXzYVoVOUUN3ME23MlE5OTRizszN Mn;LV2FPT0WU
NOS-1 M3PNV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnLrTWM2OD15LkK1PFI5KM7:TR?= M3q5bnNCVkeHUh?=
DMS-114 NFzKeGdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHTvSHpKSzVyPUeuNlk3OzFizszN MV\TRW5ITVJ?
NCI-H2030 M17RbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGO3WZVKSzVyPUeuN|M3QThizszN NWTFWmF6W0GQR1XS
HEL MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2rWNGlEPTB;Nz6zO|UzOSEQvF2= MYjTRW5ITVJ?
CAL-62 MlLNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkTHTWM2OD15LkO5PFY3KM7:TR?= MojNV2FPT0WU
COLO-668 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUWwNlExUUN3ME23MlQ{QTZ5IN88US=> MYPTRW5ITVJ?
SKG-IIIa MnnIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoLQTWM2OD15LkS1N|gyKM7:TR?= Mk\RV2FPT0WU
HCC1419 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHzTVJBKSzVyPUeuOFY5OzNizszN M{nndXNCVkeHUh?=
OS-RC-2 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFv3cnpKSzVyPUeuOFk5QTRizszN MkDsV2FPT0WU
COLO-829 Mmr4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWXJR|UxRTdwNk[0OlMh|ryP M33XfHNCVkeHUh?=
NB13 MojBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVnGR25oUUN3ME23MlgzPjZ5IN88US=> NH;HdlBUSU6JRWK=
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T98G NH3ldWtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnG4TWM2OD1|Nz64OlM2KM7:TR?= NVjyeohVW0GQR1XS
NB14 NVy0fIpUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4K1c2lEPTB;M{iuOFU1PCEQvF2= MUHTRW5ITVJ?
D-423MG M4H2UWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWDJR|UxRTN6LkS4Olkh|ryP MXHTRW5ITVJ?
NCI-H510A MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWK5XYwxUUN3ME2zPE43PDVzIN88US=> MU\TRW5ITVJ?
NMC-G1 M{S5RWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIPEWpJKSzVyPUO4MlczOjJizszN M4LObXNCVkeHUh?=
SW948 NFTRRldIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXXqU4NkUUN3ME2zPE45QTR7IN88US=> MlHlV2FPT0WU
MFM-223 M1fuTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIrOb41KSzVyPUO4Mlk1OzFizszN M{\UcXNCVkeHUh?=
PAN1 NXPsV5RsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVjJR|UxRTN6Lkm5NVkh|ryP Ml:wV2FPT0WU
MZ7-mel MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXXEfJQ5UUN3ME2zPU4yOTl7IN88US=> MUnTRW5ITVJ?
COLO-679 M37Dd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWDJRo82UUN3ME2zPU46PzB6IN88US=> M{PjWXNCVkeHUh?=
SW872 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH\RW5BKSzVyPUSwMlIxOzFizszN M4PxdnNCVkeHUh?=
HT-1197 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXrHOmtJUUN3ME20NE4zQDZ7IN88US=> NUPl[oN4W0GQR1XS
ES6 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYfK[HJ3UUN3ME20NE41PTV{IN88US=> MXjTRW5ITVJ?
SNU-387 NGnN[oxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYfJR|UxRTRyLkiyN|gh|ryP MYrTRW5ITVJ?
T47D NF\YdZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWnJR|UxRTRzLkCxPVIh|ryP NHP3WIFUSU6JRWK=
G-402 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX34dnJmUUN3ME20NU4xQDB7IN88US=> MU\TRW5ITVJ?
MZ1-PC NW[wW|RrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXvLZ3drUUN3ME20NU45OSEQvF2= M{ThR3NCVkeHUh?=
SW1417 M4PBUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn7wTWM2OD12Mj6zNFMh|ryP MVLTRW5ITVJ?
RERF-LC-MS M{\tVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlGwTWM2OD12Mj61NFg4KM7:TR?= NEHWVotUSU6JRWK=
SF539 NIDkOllIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1rSbGlEPTB;NEKuOVUzQSEQvF2= MojtV2FPT0WU
ESS-1 M4[z[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGrBUZVKSzVyPUSzMlU2PThizszN NUjnTpYyW0GQR1XS
RO82-W-1 NFjzVG9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWn5XFNQUUN3ME20N{43OTV|IN88US=> MXXTRW5ITVJ?
S-117 NWT3Wm5PT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVK1fWdwUUN3ME20N{43QTV6IN88US=> M3\ZcXNCVkeHUh?=
SW756 M2S4Zmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3zvZmlEPTB;NEOuPFI1OiEQvF2= NXK1U5h7W0GQR1XS
CP66-MEL NUHlR5lOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIPYT3dKSzVyPUS1MlcyPDNizszN NHe0RnlUSU6JRWK=
H-EMC-SS M3rEWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIfHPFVKSzVyPUS1Mlc3QTNizszN NXjO[|ZXW0GQR1XS
NCI-H2052 M2PufGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MW\JR|UxRTR3LkmwNlch|ryP MWnTRW5ITVJ?
SW684 M1LxSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIP4PHdKSzVyPUS3MlAxQDFizszN Mon3V2FPT0WU
HCC1569 M4jWbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NU\KVG1FUUN3ME20O{4yPjV6IN88US=> NXPkb2F7W0GQR1XS
KGN NEe5OVdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlrlTWM2OD12Nz6zN|EyKM7:TR?= NWnBPFhSW0GQR1XS
LCLC-103H MkDsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYTJR|UxRTR5LkS0NFQh|ryP NWrEV|FnW0GQR1XS
SW780 M{LLSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX;JR|UxRTR5Lk[zOVEh|ryP MYDTRW5ITVJ?
NCI-H1703 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MULJR|UxRTR6LkOzOVIh|ryP NVr1R|duW0GQR1XS
HCC1395 M1zQWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFjrPVVKSzVyPUS4Mlc1OzJizszN MWfTRW5ITVJ?

... Click to View More Cell Line Experimental Data

In vivo Gefitinib (100 mg/kg) improves the anti-tumor effect of radiotherapy in LoVo tumor xenografts. [3] Gefitinib treatment of nude mice bearing established human GEO colon cancer xenografts reveals a reversible dose-dependent inhibition of tumor growth because GEO tumors resumes the growth rate of controls at the end of the treatment. [4]

Protocol

Cell Research:[1]
+ Expand
  • Cell lines: NR6, NR6M and NR6W cells
  • Concentrations: 0-2 μM
  • Incubation Time: 72 hours
  • Method: Exponentially growing cells including NR6, NR6M, NR6M and NR6W cells are seeded in sextuple in 96-well plates at a concentration of 2000 cells/well, allowed to adhere and subsequently washed in PBS and incubated overnight in medium containing 0.5% FCS. Cells are then treated with varying concentrations (0-2 μM) of Gefitinib or the solute control DMSO and EGF. The optimal EGF concentration for inducing proliferation of NR6wtEGFR and NR6W cells has previously been determined and hence NR6wtEGFR and NR6W cells are supplemented with 10 nM and 0.1 nM EGF, respectively. EGF is not added to NR6 and NR6M cells. After 72 hours the amount of cells are measured by performing a MTT proliferation assay.
    (Only for Reference)
Animal Research:[3]
+ Expand
  • Animal Models: Female nude mice (cba nu/nu) aged 8–10 weeks are intra-dermal injected with LoVo cells.
  • Formulation: 0.5% polysorbate
  • Dosages: 100 mg/kg
  • Administration: Once daily by oral administration (0.1 mL/10 g body weight) for 14 days
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 89 mg/mL (199.14 mM)
Ethanol 4 mg/mL (8.95 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order:
1% CMC Na
For best results, use promptly after mixing.
30mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 446.90
Formula

C22H24ClFN4O3

CAS No. 184475-35-2
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
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    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03050164 Completed Bioequivalence Study Hunan Kelun Pharmaceutical Co., Ltd. September 8, 2016 Phase 1
NCT02856893 Not yet recruiting NSCLC European Organisation for Research and Treatment of Cancer - EORTC|AstraZeneca March 2017 Phase 2
NCT02976116 Recruiting NSCLC Hutchison Medipharma Limited December 2016 Phase 2
NCT02951637 Not yet recruiting Lung Adenocarcinoma Shanghai Chest Hospital|Ruijin Hospital|Changhai Hospital December 2016 Phase 2
NCT02930954 Not yet recruiting Non-small-cell Lung Cancer Caicun Zhou|Tongji University November 2016 Phase 2
NCT02929693 Enrolling by invitation Cancer Shanghai University of Traditional Chinese Medicine|Shanghai Chest Hospital|Shanghai Pulmonary Hospital, Shanghai, China October 2016 Phase 3

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    I try to dissolve Gefitinib (ZD1839) in 0.5% methylcellulose+0.2% Tween 80 for in vivo experiments. However, when I prepare the mixture, it forms precipitates. Exists another way to dissolve completely the compound?

  • Answer:

    S1025 can be dissovled in both 5% DMSO+corn oil and 5% DMSO+30% PEG 300+5% Tween 80+ddH2O at 2.5 mg/ml clearly. It dissolves in DMSO not readily, please sonicate and warm it in water bath at about 45 degree, and dissolve it clearly first. Then add other solvent.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID