Gefitinib (ZD1839)

Catalog No.S1025

Gefitinib (ZD1839) Chemical Structure

Molecular Weight(MW): 446.90

Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.

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Cited by 93 Publications

14 Customer Reviews

  • c, HCC4006 lung cancer cells that have acquired a high-mesenchymal state concomitant with becoming resistant to gefitinib (red curve) exhibit increased sensitivity to GPX4 inhibition.

    Nature, 2017, 547(7664):453-457. Gefitinib (ZD1839) purchased from Selleck.

    Perturbation of EGFR by its ligand EGF and gefitinib (ZD-1839 Iressat; inhibits EGFR) produces opposite responses in the predicted EGFR target genes SOCS2 and NR2E1.

    Mol Syst Biol 2011 7, 486. Gefitinib (ZD1839) purchased from Selleck.

  • Effects of shRNA-mediated RECK depletion on EGFR signaling in MEFs. (a) Immunoblotting (IB) of the indicated proteins in wild-type MEFs transduced with the indicated shRNAs and cultured for 48 h in the presence of 0.1% dimethyl sulfoxide (DMSO; vehicle) or 1 mM gefitinib (S1025, Selleck Chemicals,Houston, TX, USA). (b) Immunoprecipitation from whole lysates of cells from panel a with an anti-phosphorylated tyrosine (pTyr) antibody. Precipitates from 500 mg protein in whole cell lysates (upper) or 20 mg protein in whole cell lysates (lower) were analyzed by IB with anti-EGFR antibody.

    Oncogene 2010 30, 737-750. Gefitinib (ZD1839) purchased from Selleck.

    (B–C) LNCaP (B) and LNCaP-AI (C) cells were transiently transfected with sPLA2-IIa(-800)-Luc (0.5 μg). The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 lM) and Bortezomib (20 μM) without or with EGF (100 ng/ml) for 24 h. Luciferase assay was performed according to a standard protocol with Renilla luciferase as an internal control. Data are presented as the mean (±SD) of duplicate values of a representative experiment that was independently repeated for five times.

    Carcinogenesis 2010 31, 1948–1955 . Gefitinib (ZD1839) purchased from Selleck.

  • LNCaP-AI cells were starved in 1% stripped medium for 24 h. The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 μM) and Bortezomib (20 μM) for 24 h. Cell culture medium was collected from each sample and subjected to ELISA for sPLA2-IIa. The condition medium samples were diluted 10 times for ELISA. Average of duplicate samples was converted to nanogram per milliliter against standard curve. The data represent one of five repeated experiments.

     

     

    Carcinogenesis 2010 31, 1948–1955. Gefitinib (ZD1839) purchased from Selleck.

    Suppression of EGFR signaling by Gefitinib is dose dependent and non- toxic at millimolar concentrations.Results from plaque reduction tests of Hep2 cells infected with VACV(12.5pfu/well) treated with a serial dilution of Gefitinib (1000-0.01 m) and analyzed for (A)proliferation as indicator of cytotoxicity,(B) IC50 of plaque size inhibition, (C)dose dependent inhibition of EGFR-ERK1/2signaling and VACV proteins by Western blot analysis (pEGFR detection was obtained from a different experiment with identicals ettings) and (D)dose dependent inhibition of orthopoxvirus genome replication by real-time PCR.Untreated VACV infectedcells and uninfected cells are shown as controls in the right panel.

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

  • A circular zone of EGFR activated but uninfected cells facilitate virus induced plaque development , efficiently blocked by Gefitinib. (A)Immun of luorescent staining of aplaque reduction tests with Hep2 cells 24,48,72 and 96 h after VACV infection (6.25pfu/chamber). VACV infected cells(green) were visualized by human IgG directed to orthopoxviruses (VIG) detected with a secondary FITC labelled antibody. Uninfected cells with activated EGFR signaling(red) are detected with a secondary Cy3labelled antibody. Positive cells for both , VACV and phosphorylated EGFR are shown in yellow(merge). Cellular nuclei are counterstained with DAPI. (B)Immunofluorescent staining of a plaque reduction test as described for (A) and treated with three different Gefitinib concentrations (1000 nM, 1 nM, and 0.01 nM) . The test was analyzed 96h afterVACV infection.Uninfected cells are shown as controls. Magnification100×.(For interpretation of the references to color in this figure legend , the reader is referred to the web version of the article.)

     

     

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

    Susceptibility of lung cancer cells to cytolytic activity of NK-92 cells after treatment with EGFR inhibitors. Three lung cancer cells were untreated (open circle) or treated with 10 μM erlotinib or gefitinib (black filled square or black filled triangle) for 24 hours. The lung cancer cells were cocultured with NK-92 cells at indicated effector to target ratio (E:T ratio). To determine the specificity of NKG2D-mediated cytolysis of lung cancer cells, NK-92 cells were preincubated with blocking mAb against NKG2D before the assay (gray filled square and triangle) and correspond-ing isotype mAb (open square and triangle).

    J Immunother 2011 34(4), 372-81. Gefitinib (ZD1839) purchased from Selleck.

  • Inhibition of 5-HT-induced 5-HT3A and 5-HT3AB receptor currents.

    Eur J Pharmacol, 2017, 821:21-28. Gefitinib (ZD1839) purchased from Selleck.

    The EGF-dependent increased association of PCM1 and AZI1 requires EGFR kinase activity. ZD1839 (Iressa)—EGFR tyrosine kinase inhibitor.

    Mol Biosyst 2011 7, 3223-33. Gefitinib (ZD1839) purchased from Selleck.

  • Reduction of GPER expression in HCC1806 cells after treatment with gefitinib. (A) Concentration dependence. HCC1806 cells were treated with 50-500 nM gefitinib for 48 h. GPER was detected by western blotting. (B) Time dependence. HCC1806 cells were treated with 200 nM gefitinib for 24-96 h. ap<0.05 vs. control; bp<0.001 vs. control. Mean values and SE of three independent experiments are shown. (C) Representative western blotting of concentration dependence. (D) Representative western blotting of time dependence

    Oncol Rep, 2017, 37(2):1212-1218. Gefitinib (ZD1839) purchased from Selleck.

    Inhibition of signaling pathway activation in lung tumor cell lines by kinase inhibitors. Lung tumor cells were cultured in 10% FBS until reaching ∼80% confluence and then the cells were starved in serum-free medium for overnight, followed by 4-hour treatment with the inhibitors. Cell lysates were then prepared and used for determination of the pathway activation signals by the CEER assay.

    Int J Proteomics 2011 215496. Gefitinib (ZD1839) purchased from Selleck.

  • Breast cancer cells were pretreated with 100ng/ml EGF for 15 min and then treated with the indicated concentrations of  Gefitinib for 24 hours.

     

     

    2010 Dr. Zhang of Tianjin Medical University. Gefitinib (ZD1839) purchased from Selleck.

    MTT assay result. 0-500nM Gefitinib treated with H1299, H358, H25 and HCC827 cell lines by 72h, Proliferation rate was tested.

     

     

    2010 Dr. Vicky Tin from University of Hong Kong. Gefitinib (ZD1839) purchased from Selleck.

Purity & Quality Control

Choose Selective EGFR Inhibitors

Biological Activity

Description Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.
Features A potent EGFR tyrosine kinase inhibitor.
Targets
Tyr1173 (NR6W cells) [1] Tyr1173 (NR6wtEGFR cells) [1] Tyr992 (NR6wtEGFR cells) [1] Tyr992 (NR6W cells) [1]
26 nM 37 nM 37 nM 57 nM
In vitro

Gefitinib effectively inhibits all tyrosine phosphorylation sites on EGFR in both the high and low-EGFR-expressing cell lines including NR6, NR6M and NR6W cell lines. The phosphorylation sites Tyr1173 and Tyr992 are less sensitive requiring higher concentrations of Gefitinib for inhibition. Gefitinib effectively blocks the phosphorylation of PLC-γ, with IC50 of 27nM, in NR6W cells. The NR6wtEGFR and NR6M cell lines has low levels of PLC-γ phosphorylation but the level in the NR6M cell line is more resistant to inhibition by Gefitinib with IC50 of 43 nM and 369 nM, respectively. Gefitinib inhibits Akt phosphorylations, with IC50 of 220 and 263nM, in the low-EGFR- and -EGFRvIII-expressing cell lines, respectively. Gefitinib in the dose range from 0.1 to 0.5μM significantly facilitates, rather than abrogates, colony formation of NR6M cells. However, at a concentration of 2 μM Gefitinib completely blocks NR6M colony formation. Gefitinib rapidly and in a dose-dependent manner inhibits EGFR and ERK phosphorylation up to 72 hours after EGF stimulation in both the high- and low-EGFR-expressing cell lines. [1] Gefitinib is the monolayer growth of these EGF-driven untransformed MCF10A cells with an IC50 of 20 nM. [2] The combination of Gefitinib (0.2 μM and 0.5 μM) with irradiation lead to a significant growth inhibition in LoVo cells, compared with radiation alone.. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KB NW[1fmpHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlLOTWM2OD1yLkC1OEDPxE1? MU[xOlUyPjR5Mx?=
NIH3T3 MknYSpVv[3Srb36gRZN{[Xl? MlPNNE4zKM7:TR?= Mo\mNkBp NWH6PYpkTE2VTx?= MmLVTY5pcWKrdHnvckBw\iCHR1[gd5RqdXWuYYTl[EBpfW2jbjDldoJDOSCjdYTvdIhwe3Cqb4L5cIF1cW:wIIfpeIghUUN3MDDv[kAxNjBzNESg{txO NG\IZ2cyPjR6MEK4OC=>
COS7 M2HU[WZ2dmO2aX;uJGF{e2G7 M33nU|k3KM7:TR?= M331OGlvcGmkaYTpc44hd2ZiaIXtZY4hUkGNMjDWOlE4TiCvdYThcpQh\XiycnXzd4Vl NWS0[GE4OTdzN{i3NlI>
A549 NGrXe5NEgXSxdH;4bYMhSXO|YYm= MnjkPVYhcA>? MoH2SG1UVw>? NVzocWFpUUN3ME2xN{42QSEQvF2= M364bVE5OzF|OEC3
K562 NG\vRm1EgXSxdH;4bYMhSXO|YYm= NWS0[oQyQTZiaB?= NV\EPItqTE2VTx?= NUjSNYNLUUN3ME25MlM3KM7:TR?= MUGxPFMyOzhyNx?=
MDA-MB-231 MVLDfZRwfG:6aXOgRZN{[Xl? NIO0V2o6PiCq MoWzSG1UVw>? MoPaTWM2OD1|Nj62NUDPxE1? NYLBc|RxOTh|MUO4NFc>
MOLT4 NXLFSIVSS3m2b4TvfIlkKEG|c3H5 MUC5OkBp NIi2OphFVVOR MVzJR|UxRTF3LkCyJO69VQ>? NEP4PZAyQDNzM{iwOy=>
MCF7 MWXDfZRwfG:6aXOgRZN{[Xl? MnLYOVAh|ryP NICxbVY4OiCq MXfJR|UxRTF{LkC1JO69VQ>? MkHiNVg4PzF6MUm=
BT474 M2LHcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH34flY4OiCq NEHMRnRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFLUOFc1KGOnbHzzJI93\XKneIDy[ZN{cW6pIFXSRoIzKHerdHigSWM2OCCxZjCwMlMh|ryP NIm5UnYyQTB{OESyOC=>
HN5 NFm3eo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2nLUlczKGh? M1PwO2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSF61JINmdGy|IH;2[ZJmgHC{ZYPzbY5oKEWJRmKge4l1cCCHQ{WwJI9nKDBwMEGg{txO NXvwTWJ4OTlyMki0NlQ>
BA/F3 NEnybldEgXSxdH;4bYMhSXO|YYm= NH;Iem84OiCq M4KzeGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJGJCN0Z|IHPlcIx{KGW6cILld5NqdmdiRVfGVkBNQDV6UjDteZRidnRid3n0bEBKSzVyIH;mJFAvODFyODFOwG0> M1X3PFE6OjN7MkK5
NIH3T3 M4T1Tmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MU[3NkBp NGPLcFBIem:5dHigbY5pcWKrdHnvckBw\iCvb4Xz[UBPUUhxM2SzJINmdGy|IHX4dJJme3Orbneg[ZJDOiCpZX7lJJdqfGhiSVO1NEBw\iByLkW0JO69VQ>? MlfINVk3PjV|N{e=
MCF7 MX MkDVSpVv[3Srb36gRZN{[Xl? NFnxRoc{OCCvaX6= MVvJcohq[mm2aX;uJI9nKEKFUmCg[ZhxemW|c3XkJJdqfGhiSVO1NEBw\iBzLkGg{txO NHS5fWkyQTl|Mkm2NC=>
A431 NGPIcW9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NID0OmwyOCEQvF2= NHrEZVU4OiCq MlLJS2k2OD1yLkWxOEDPxE1? MnXjNVk6Pjl2NkW=
A431 M4LZU2tqdmG|ZTDBd5NigQ>? M{nCd|AvOSEQvF2= MYixJIg> NFy3TGdKdmirYnn0bY9vKG:oIFXHSk1qdmS3Y3XkJGVITlJicHjvd5Bpd3K7bHH0bY9v NGTidm0yQTl4OUS2OS=>
A431 M1ez[WtqdmG|ZTDBd5NigQ>? NGG3NFgyODBizszN MoDzNVAhdWmw MljWVJJwdG:wZ3XkJIlvcGmkaYTvdpkh[WO2aY\peJkhd2ZiRVfGMYlv\HWlZXSgSWdHWiCyaH;zdIhwenmuYYTpc44> M1yyS|E6QTZ7NE[1
Sf9 M2fVSGtqdmG|ZTDBd5NigQ>? M2\1UFExKG2rbh?= M1\VeWROW09? NWG4VJBtUW6qaXLpeIlwdiCxZjDFS2ZTKGW6cILld5Nm\CCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKHKnY3XweI9zKGG3dH;wbI9{eGixconsZZRqd25id3n0bEBKSzVyIH;mJFAvODN|IN88US=> NVrMZYRvOjByNU[0NlU>
NCI-H1975 Mn7pS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmLmOUDPxE1? M1zSPVczKGh? NV7KOJFHUUN3ME24MlI3KM7:TR?= M4S0SVIxOTVzNkew
NCI-H1975 M3GydWtqdmG|ZTDBd5NigQ>? MkLrNVAh|ryP M3niU2lvcGmkaYTpc44hd2ZiRVfGVkBifXSxcHjvd5Bpd3K7bHH0bY9v MoPKNlAyPTF4N{C=
NCI-H1975 MoTnSpVv[3Srb36gRZN{[Xl? MkO4NVAh|ryP MlzBTY5pcWKrdHnvckBw\iCHUlLCNkBifXSxcHjvd5Bpd3K7bHH0bY9v MYSyNFE2OTZ5MB?=
NCI-H1975 NWrWVGtbTnWwY4Tpc44hSXO|YYm= NWTLbmhQPSEQvF2= NELSXGc1QCCq NWXMVnN7UW6mdXP0bY9vKG:oIHHwc5B1d3OrczDhd5Nme3OnZDDhd{Bi[3SrdnH0bY9vKG:oIHPhd5Bie2VvMx?= NH;kSY8zODF3MU[3NC=>
A375 NIXJRnZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUe0PEBp MXPJR|UxRTJyLkW1JO69VQ>? M4Lmc|IxOzB2NUO3
SMMC7721 NF7sRYdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmHuOFghcA>? NFKz[VNKSzVyPUOzMlEzKM7:TR?= MkexNlA{ODR3M{e=
DU145 NVO1W|NGS3m2b4TvfIlkKEG|c3H5 NH3tT3o2KGR? MkDFSG1UVw>? NUSxfFBoUUN3ME20Nk4yPyEQvF2= NYjFZYFJOjB2Nk[1OVU>
MIAPaCa2 NFfQNFFEgXSxdH;4bYMhSXO|YYm= MWq1JIQ> MnjBSG1UVw>? MmjrTWM2OD13MD62NkDPxE1? M3HLWlIxPDZ4NUW1
PANC1 NIDWWWFEgXSxdH;4bYMhSXO|YYm= M37ESFUh\A>? M3T2WWROW09? NYnBT3NQUUN3ME20NE41OiEQvF2= NEDMXmwzODR4NkW1OS=>
PC9 NHvCdndIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGTWO5BCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGVITlJvZHXmbYNq\W62IHj1cYFvKFCFOTDj[YxteyC5aYToJGVEPTBib3[gNE4xOTdizszN Ml3kNlEzODh6MEK=
PC9 NFrhdpNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHfyc5d1cC2{ZYPpd5RidnRiaIXtZY4hWEN7IHPlcIx{KGW6cILld5NqdmdiRVfGVkBGPzR4X1G3OVAwXDd7MF2gcZV1[W62IIfpeIghTUN3MDDv[kA4KM7:TR?= NV3oVm5nOjF{MEi4NFI>
PC3 M2TMW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUOxNEDPxE1? M136OVczKGh? MYXEUXNQ MWXJR|UxRTdwNDFOwG0> MlL0NlE{PTN3NE[=
MDA-MB-468 MlP4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX2xNFAh|ryP M3\M[VQ5KGh? MlzHSG1UVw>? NGjibJhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fS=> M1XGe|IyPDJ7NkOy
Plasmodium falciparum MlTWdWhVWyCycn;mbYxqdmdiZn;yJIlvcGmkaYTvdpMhd2ZiUHzhd41w\Gm3bTDmZYxkcXCjcoXtJJBzd2yrZnXyZZRqd25? M4PpdVIyQDF5MES1
NCI-H1993 NHvqfJRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkfiO|IhcA>? MmTpSG1UVw>? NUD6OIhOUUN3ME2wMlEh|ryP NF7RRW4zOTlzNkSzNy=>
NCI-H292 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFvIOlU4OiCq Ml3YSG1UVw>? MnziTWM2OD1yLkCyJO69VQ>? MljKNlE6OTZ2M{O=
A431 MlvCT4lv[XOnIFHzd4F6 NXjTblQyOiCq Mki5SG1UVw>? M33DXWlvcGmkaYTpc44hd2ZiRVfGVkBxcG:|cHjvdplt[XSrb36gbY4hTUeILYP0bY12dGG2ZXSgbJVu[W5iQUSzNUB4cXSqIFnDOVAhd2ZiMD6wNFEh|ryP MnHnNlIyODFzM{K=
MCF7 NUXsTnNFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUSwTJhqOTBywrFOwIcwdUx? NEjDdYk1QCCq Mkf0SG1UVw>? M4H0cWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDveoVz\XiycnXzd4lv\yCHR1\SJIdmdmVid3n0bEBKSzVyIH;mJFEzNjB3IN88US=> NWSxTnJYOjJzMUmxN|A>
BT474 MUDDfZRwfG:6aXOgRZN{[Xl? NV\VOJF2OTBizszN MUe0PEBp MXnDfZRwfG:6aXPpeJkh[WejaX7zeEB{\XK3bT3zeIFzfmWmIHj1cYFvKEKWNEe0JINmdGy| MVeyNlE{QDNyOB?=
MCF7 NYS5c|BYS3m2b4TvfIlkKEG|c3H5 MmjONVAh|ryP M2K5[VQ5KGh? NXH2U3AxS3m2b4TvfIlkcXS7IHHnZYlve3Ric3XyeY0ue3Sjco\l[EBpfW2jbjDNR2Y4KGOnbHzzJI93\XJvZYjwdoV{e2mwZzDFdoJDOiClZXzsdy=> M3X4ZlIzOTN6M{C4
HCC827 NXXpSXI1U2mwYYPlJGF{e2G7 MVOwMlEzPSEQvF2= NY\NcppsOjRiaB?= MYXEUXNQ NHjkfWZKdmirYnn0bY9vKG:oIFXHSnIh\GWuIFW3OFYuSTd3MDDteZRidnRiWUGwOlgh[XW2b4Doc5NxcG:{eXzheIlwdg>? Mk\4NlI{Ozl|NEK=
HCC827 MU\LbY5ie2ViQYPzZZk> M2HPSFAvOTJ3IN88US=> MU[yOEBp M2nJV2ROW09? MW\Jcohq[mm2aX;uJI9nKEWJRmKg[IVtKEV5NE[tRVc2OCCvdYThcpQudWWmaXH0[YQhTXKtIIDoc5NxcG:{eXzheIlwdg>? M1;zZVIzOzN7M{Sy
HCC827 NY\yZ25CU2mwYYPlJGF{e2G7 MWGwMlEzPSEQvF2= NX;GWVlVOjRiaB?= Ml7oSG1UVw>? MnLLTY5pcWKrdHnvckBw\iCHR1\SJIRmdCCHN{S2MWE4PTBibYX0ZY51NW2nZHnheIVlKEGtdDDwbI9{eGixconsZZRqd25? MV[yNlM{QTN2Mh?=
A431 MmC0T4lv[XOnIFHzd4F6 MmnXNVAxKM7:TR?= MkKxN|AhdWmw M1T6NGlvcGmkaYTpc44hd2ZiRVfGVkBl\XKrdnXkJIZzd21iaIXtZY4hSTR|MTDj[YxtKG2nbXLyZY5mKGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YheG:ueT3HcJUuXHm{IIDoc5NxcG:{eXzheIlwdiC5aYToJGlEPTBib3[gNU4xQCEQvF2= NF;ZW|kzOjZ7OEe4Ni=>
H460 NIr1d4pEgXSxdH;4bYMhSXO|YYm= NF;tUoM4OiCq MUPJR|UxRTVwNUmg{txO MnSxNlI4Ojd2NEm=
HT-29 Ml\YR5l1d3SxeHnjJGF{e2G7 MVq3NkBp MUDJR|UxRTNwM{[g{txO M{jl[|IzPzJ5NES5
SGC7901 M1\ZPGN6fG:2b4jpZ{BCe3OjeR?= Ml;VO|IhcA>? NHrESYlKSzVyPUGwMlI3KM7:TR?= MkKxNlI4Ojd2NEm=
A498 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEF2OUigZ4VtdHNiZYjwdoV{e2mwZzDFS2ZTKGGwZDDTVmMhf2m2aDDJR|UxKG:oIEiuOkDPxE1? M4rKUFIzQDF6OES4
Calu3 NXzOeIV{S3m2b4TvfIlkKEG|c3H5 NFXBblIyOCEQvHevcWw> NHm1UYc4OiCq MmTYTWM2OD1zLk[g{txO MVKyN|EyPjF4OB?=
FADU NEPVN2REgXSxdH;4bYMhSXO|YYm= NFfFTmEyOCEQvHevcWw> MmnjO|IhcA>? NV21NI1VUUN3ME2wMlA2KM7:TR?= M1jCbVI{OTF4MU[4
HCC827 MoLlR5l1d3SxeHnjJGF{e2G7 MYmxNEDPxGdxbVy= NWL6TFBGPzJiaB?= MUXJR|UxRTBwMECxOUDPxE1? Ml7sNlMyOTZzNki=
PC9 M17DXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV3GT|ROOTBizsznM41N NGTvOJg4OiCq NInD[ldKSzVyPUCuNFAyPCEQvF2= NFj2cpkzOzFzNkG2PC=>
Sf9 Moq3T4lv[XOnIFHzd4F6 NFHocmwyKGh? MkHZSG1UVw>? MkP2TY5pcWKrdHnvckBw\iCqdX3hckBIW1RvdHHn[4VlKEWJRmKgUFg{PFJibYX0ZY51KGW6cILld5Nm\CC5aYToJGlEPTBib3[gNE4xOTVizszN NYfBOZgyOjN4MUG2PVE>
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BT-20 NGnse3FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M37UdGlEPTB;Mj6yOlc1PCEQvF2= MWXTRW5ITVJ?
A498 M4\rOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmP3TWM2OD1{LkK5OFE2KM7:TR?= MXvTRW5ITVJ?
IGROV-1 MoHiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MonsTWM2OD1{LkOxNlM4KM7:TR?= NX[xOYpQW0GQR1XS
JVM-3 M4PER2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYL4XXVMUUN3ME2yMlM1PjR|IN88US=> MYDTRW5ITVJ?
NCI-H2170 NFn2cHlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWjxcmh5UUN3ME2yMlM1Pzh|IN88US=> NFXDUVZUSU6JRWK=
EW-18 M37GeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFj1ZWRKSzVyPUKuN|U1PzFizszN NYCyO3VGW0GQR1XS
KU812 NVLPSIN1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF;W[HVKSzVyPUKuN|Y4PzlizszN MUDTRW5ITVJ?
NY MkD5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkTHTWM2OD1{LkO3Nlch|ryP NGDKZXRUSU6JRWK=
HTC-C3 MlXnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWXJR|UxRTJwNEKyOlch|ryP NFrLS2FUSU6JRWK=
CFPAC-1 Ml\jS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV3JR|UxRTJwNEO4OlQh|ryP MVPTRW5ITVJ?
HH NXS5OIhPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2fue2lEPTB;Mj60PVg2PCEQvF2= M3WycnNCVkeHUh?=
MKN7 Mki0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXXJR|UxRTJwNkK3OFgh|ryP NWfMXHVkW0GQR1XS
TE-8 M4\5RWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXfUZ|NkUUN3ME2yMlY{PjV|IN88US=> Mm\qV2FPT0WU
MC-IXC M{HWeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mnf2TWM2OD1{Lk[0OFU{KM7:TR?= NFjyOoRUSU6JRWK=
DMS-79 NIfUb5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGPJWVdKSzVyPUKuOlkzQDRizszN M2PRfXNCVkeHUh?=
Detroit562 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mnj2TWM2OD1{Lk[5PFQ2KM7:TR?= NYP0O3VEW0GQR1XS
HuP-T4 NH;CcHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2O0emlEPTB;Mj63NlgyQCEQvF2= NEfsR49USU6JRWK=
QIMR-WIL M{XwSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFvndJpKSzVyPUKuO|U6PTJizszN M3LVcnNCVkeHUh?=
BB65-RCC NGj0RXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWHnNFRKUUN3ME2yMlgxPzF|IN88US=> MYDTRW5ITVJ?
PC-3 M3XVfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlPJTWM2OD1{LkizN|E4KM7:TR?= Mn6xV2FPT0WU
OVCAR-3 M1TWfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUHJR|UxRTJwOEW1PVQh|ryP MnPCV2FPT0WU
KYSE-510 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NETBNWVKSzVyPUKuPFY4QDZizszN MkjiV2FPT0WU
HC-1 MlzTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGLNfFJKSzVyPUKuPVQxOTJizszN MULTRW5ITVJ?
NCI-H1693 M3zGXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2D6SGlEPTB;Mj65OVg6PyEQvF2= NIfJRVdUSU6JRWK=
DU-4475 M2P1Umdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3PoeGlEPTB;Mj65PVA2OiEQvF2= MX3TRW5ITVJ?
COLO-680N MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4\icGlEPTB;Mz6wNFQ6PSEQvF2= M4H2dHNCVkeHUh?=
MN-60 NYnJNmc2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHvkR4VKSzVyPUOuNFQ6PzRizszN NWnNb|lNW0GQR1XS
BCPAP M2\Le2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1W4dmlEPTB;Mz6wPFI6PSEQvF2= MXnTRW5ITVJ?
647-V MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml;kTWM2OD1|LkG2NlIh|ryP MXPTRW5ITVJ?
HT-1376 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXvJR|UxRTNwMkC1NFYh|ryP MY\TRW5ITVJ?
NCI-H1793 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYDJR|UxRTNwMkGwPVgh|ryP NXr2N2VWW0GQR1XS
SW1463 M4D2VGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2TOR2lEPTB;Mz6yOlE6OiEQvF2= NHn4dVlUSU6JRWK=
NCI-H727 M4Prbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnrxTWM2OD1|LkO4OVg1KM7:TR?= MnHYV2FPT0WU
LB771-HNC M322NGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn6zTWM2OD1|LkO5NVI3KM7:TR?= Mkj5V2FPT0WU
D-542MG NGTB[oZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXXJR|UxRTNwNESyNlIh|ryP NIr5OYlUSU6JRWK=
NTERA-S-cl-D1 NUi4eJZmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{XTbWlEPTB;Mz60OFk2PyEQvF2= MlXVV2FPT0WU
C8166 NFfNNHlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4XEeGlEPTB;Mz60OlUzOSEQvF2= M1PsfHNCVkeHUh?=
639-V Mn[yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2jDNmlEPTB;Mz61O|c6OiEQvF2= NHrseGFUSU6JRWK=
TE-1 NHywblZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYDJR|UxRTNwNUmxOlkh|ryP NIjuXmhUSU6JRWK=
OAW-28 Mki5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2Djc2lEPTB;Mz61PVQzPiEQvF2= NVXEcHhjW0GQR1XS
SK-LU-1 M3TOUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV;JR|UxRTNwNkC2OFUh|ryP Mn;vV2FPT0WU
CaR-1 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWfJR|UxRTNwNkGwOFgh|ryP NYWzVphNW0GQR1XS
A2780 NFPzWWhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXXJR|UxRTNwNkSwPFkh|ryP MXnTRW5ITVJ?
C2BBe1 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYrJR|UxRTNwNkS0NFch|ryP NVrhfIxKW0GQR1XS
SK-MES-1 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX7JR|UxRTNwNkS2NVQh|ryP M{e2UnNCVkeHUh?=
PANC-10-05 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M13DTmlEPTB;Mz63NlA4OiEQvF2= M3jzW3NCVkeHUh?=
J-RT3-T3-5 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYrpTpBoUUN3ME2zMlc2PDB4IN88US=> MmXSV2FPT0WU
ES4 M17rNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NInRN4tKSzVyPUOuPFIzQDZizszN MYPTRW5ITVJ?
NCI-H28 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF:z[YtKSzVyPUOuPFI6PjFizszN NGjxSVFUSU6JRWK=
NCI-H1155 NGHSXVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVPYfIQ5UUN3ME2zMlg1OTV7IN88US=> MnqxV2FPT0WU
KURAMOCHI NIX2OHpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXPJR|UxRTNwOE[wOVYh|ryP NIjZTpJUSU6JRWK=
NCI-H69 NHTOfllIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4DBO2lEPTB;Mz65NFE4QCEQvF2= NWTLc|ExW0GQR1XS
IST-MES1 NELmT21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1jNdmlEPTB;Mz65OFAzQSEQvF2= M{DKenNCVkeHUh?=
NH-12 M{nEeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWjnSXVmUUN3ME2zMlk1OTh5IN88US=> NIfqeIxUSU6JRWK=
JVM-2 NXnQVJBCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIq0dldKSzVyPUOuPVQ4PTJizszN MUXTRW5ITVJ?
EW-16 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF;rVmRKSzVyPUOuPVU2PzdizszN NF7vT|FUSU6JRWK=
NCI-H441 M4DTTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn;lTWM2OD1|Lkm3NFE1KM7:TR?= M4DVUHNCVkeHUh?=
RCC10RGB M1;kZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4DpVGlEPTB;ND6wNVI1PiEQvF2= NXXveXkzW0GQR1XS
TCCSUP MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3vHd2lEPTB;ND6xNVA2KM7:TR?= NHnre|RUSU6JRWK=
HL-60 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX3PfXFNUUN3ME20MlEzOjRzIN88US=> M1nWb3NCVkeHUh?=
EW-11 NEHBRlFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{nkPWlEPTB;ND6xN|I3OSEQvF2= MlzYV2FPT0WU
KARPAS-299 MoHvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mm\wTWM2OD12LkOxNFE{KM7:TR?= MYrTRW5ITVJ?
SNU-C2B MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIT0V4xKSzVyPUSuN|Q3QDZizszN M4PvNnNCVkeHUh?=
LU-139 NF7tPFBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIKxVnFKSzVyPUSuN|czPDdizszN Mkf2V2FPT0WU
HCC1937 NHXDSIVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYDxcIVqUUN3ME20MlM4QTl|IN88US=> Mk\NV2FPT0WU
GR-ST MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUHJO2huUUN3ME20MlQ{OThizszN MoG1V2FPT0WU
SK-OV-3 NFmwS5RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHLaeJhKSzVyPUSuOFYxPDJizszN MXHTRW5ITVJ?
A172 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWjtbXA{UUN3ME20MlU2PTJzIN88US=> MYnTRW5ITVJ?
NB10 NE[0UopIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX;JR|UxRTRwNUm3PFEh|ryP NUmyNVBqW0GQR1XS
P30-OHK MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MorXTWM2OD12LkixPVI6KM7:TR?= MWLTRW5ITVJ?
KYSE-520 Mmq4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mk\ZTWM2OD12Lki4NFE5KM7:TR?= NFi3O3pUSU6JRWK=
KNS-62 NYDrcZBvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYWzfolnUUN3ME20MlkxQDB5IN88US=> M2nlRnNCVkeHUh?=
ES1 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWjJR|UxRTRwOU[wN|Uh|ryP MX7TRW5ITVJ?
KOSC-2 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1;4OGlEPTB;ND65PVk2QSEQvF2= NG\weoZUSU6JRWK=
TE-9 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWLJR|UxRTVwMEG1NlUh|ryP M{LSOnNCVkeHUh?=
NCI-H1838 M1vnb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUnnOYxVUUN3ME21MlAzODd4IN88US=> NUnMUIFUW0GQR1XS
KP-N-YN MmezS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXzJR|UxRTVwMUK1PVEh|ryP MUPTRW5ITVJ?
ALL-PO NH7xZ5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3rLUWlEPTB;NT6yNlU5PyEQvF2= M3nhPXNCVkeHUh?=
NOMO-1 MoTsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1SzfGlEPTB;NT6yN|E4PiEQvF2= MXXTRW5ITVJ?
CGTH-W-1 MlfmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEjwUJFKSzVyPUWuN|g2QDRizszN MlLaV2FPT0WU
ATN-1 Ml;NS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX\JPHV[UUN3ME21MlQyOTh2IN88US=> M37kVXNCVkeHUh?=
AGS NXTqSVZUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MontTWM2OD13LkS5OlE5KM7:TR?= MlTQV2FPT0WU
SW13 NIDleoxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV\JR|UxRTVwNUOyOlUh|ryP MVfTRW5ITVJ?
SW1990 Mk\IS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MY\JR|UxRTVwNkKyPFUh|ryP MXHTRW5ITVJ?
BFTC-905 NUT0SYN3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1fxN2lEPTB;NT62OVg4QCEQvF2= M{jrTnNCVkeHUh?=
RMG-I NFzFXoxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmDyTWM2OD13Lk[1PVQzKM7:TR?= MXnTRW5ITVJ?
HD-MY-Z MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoPGTWM2OD13Lk[4OVgyKM7:TR?= MkHXV2FPT0WU
HCC1187 NUDTWphsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3vpVmlEPTB;NT63NVA{OiEQvF2= MkXYV2FPT0WU
HCC2998 MmD5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1HRbWlEPTB;NT63NVg4PyEQvF2= MVvTRW5ITVJ?
RT-112 M{HuO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1zMVGlEPTB;NT65NFI1PSEQvF2= Mn[5V2FPT0WU
CAKI-1 M3vY[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MorWTWM2OD14LkCwPVg2KM7:TR?= NGi4[lhUSU6JRWK=
HDLM-2 M3;SOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFj2c3hKSzVyPU[uNFE1QDlizszN NULLfXljW0GQR1XS
TE-10 MkX1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYHlU4hOUUN3ME22MlA1QDN|IN88US=> M13kVXNCVkeHUh?=
KE-37 MnvIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVu4OVhMUUN3ME22MlA4OjV6IN88US=> NVW3SIlRW0GQR1XS
RCM-1 NXL6ZWFjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NG\yNI9KSzVyPU[uNFc4OTlizszN NVvBboJlW0GQR1XS
Mewo NGrDZVhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2jsdmlEPTB;Nj6xNVQ5PyEQvF2= NXjMdY5NW0GQR1XS
L-428 MkntS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NE\jfohKSzVyPU[uNVgxOzdizszN M1fwcHNCVkeHUh?=
8-MG-BA MlLGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV62cJU4UUN3ME22MlE6Ozd7IN88US=> MkC2V2FPT0WU
SK-MEL-28 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYHJR|UxRTZwMkC5Olch|ryP MnjkV2FPT0WU
VA-ES-BJ NH74WmtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4jGXmlEPTB;Nj6zNFQ3OiEQvF2= MkfBV2FPT0WU
CAL-120 MlfBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1nKSGlEPTB;Nj60OVE3OiEQvF2= M4PBPXNCVkeHUh?=
GAMG M3frS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVXFXFEyUUN3ME22MlUxPjJizszN NV;jdmduW0GQR1XS
MSTO-221H MkXkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmK0TWM2OD14LkWxNVch|ryP MkexV2FPT0WU
GCT M3vJTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4K5PWlEPTB;Nj61N|M5PyEQvF2= NV3ENVhnW0GQR1XS
SH-4 NUi0cXVXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV;JR|UxRTZwNUi4OFQh|ryP NFqyTYVUSU6JRWK=
Calu-3 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH\UPGtKSzVyPU[uOlU2PjhizszN M{H1dHNCVkeHUh?=
SK-HEP-1 M2LYcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYLQXpJUUUN3ME22MlcyPzV|IN88US=> MmqxV2FPT0WU
PFSK-1 M1zre2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX3JR|UxRTZwN{O0O|gh|ryP Ml3lV2FPT0WU
NB12 NHXNSmdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NU\acZo2UUN3ME22Mlc5PDB2IN88US=> NV\FZow3W0GQR1XS
A388 MkTBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYDiToJvUUN3ME22Mlg6PTJizszN NWHMeldpW0GQR1XS
KLE M3fwd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX\JR|UxRTZwOU[1NVYh|ryP NHrVVJZUSU6JRWK=
TE-11 NV\l[Y5yT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml7wTWM2OD15LkC0PVY4KM7:TR?= MlLDV2FPT0WU
NCI-H2405 M2XBXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mk\0TWM2OD15LkC4N|k{KM7:TR?= NFfYR5BUSU6JRWK=
OVCAR-4 M2PVfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXnJR|UxRTdwMUWzOUDPxE1? Ml\BV2FPT0WU
KP-N-YS MmjCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH;RV5lKSzVyPUeuNVgyPCEQvF2= NUjqPIlEW0GQR1XS
NOS-1 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2rUemlEPTB;Nz6yOVgzQCEQvF2= MmfyV2FPT0WU
DMS-114 MknqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4DUUmlEPTB;Nz6yPVY{OSEQvF2= MkOyV2FPT0WU
NCI-H2030 NXPINGtUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXjJR|UxRTdwM{O2PVgh|ryP M1vDO3NCVkeHUh?=
HEL MoXlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVzwWGdnUUN3ME23MlM4PTJzIN88US=> M4\oPXNCVkeHUh?=
CAL-62 MnXSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYfSWIlsUUN3ME23MlM6QDZ4IN88US=> NVn5dYhYW0GQR1XS
COLO-668 M3;4[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlnVTWM2OD15LkSzPVY4KM7:TR?= MoXEV2FPT0WU
SKG-IIIa M4La[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{fwd2lEPTB;Nz60OVM5OSEQvF2= NHXyNYFUSU6JRWK=
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T98G NH3xblRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3PVeGlEPTB;M{euPFY{PSEQvF2= MVfTRW5ITVJ?
NB14 MnjFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MknPTWM2OD1|OD60OVQ1KM7:TR?= MWDTRW5ITVJ?
D-423MG MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn;KTWM2OD1|OD60PFY6KM7:TR?= NH7hT5NUSU6JRWK=
NCI-H510A NVq2Z|BQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXu2RoRzUUN3ME2zPE43PDVzIN88US=> M4\nc3NCVkeHUh?=
NMC-G1 NUDQNGhMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NELOfGxKSzVyPUO4MlczOjJizszN NGn1TWZUSU6JRWK=
SW948 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIe2T2NKSzVyPUO4Mlg6PDlizszN MX3TRW5ITVJ?
MFM-223 M3XaXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV;LNItLUUN3ME2zPE46PDNzIN88US=> NVjidoNTW0GQR1XS
PAN1 NGfXN|ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1fhfGlEPTB;M{iuPVkyQSEQvF2= NXvrXlJpW0GQR1XS
MZ7-mel NVPPcHBWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{HnR2lEPTB;M{muNVE6QSEQvF2= M2fXbnNCVkeHUh?=
COLO-679 M4XSWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoPKTWM2OD1|OT65O|A5KM7:TR?= NWS3flJuW0GQR1XS
SW872 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3PSN2lEPTB;NECuNlA{OSEQvF2= M{D4TXNCVkeHUh?=
HT-1197 M2HPXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGTEOWlKSzVyPUSwMlI5PjlizszN MUnTRW5ITVJ?
ES6 NIrtbXBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWPJR|UxRTRyLkS1OVIh|ryP MonEV2FPT0WU
SNU-387 NXezUGIxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mk\pTWM2OD12MD64NlM5KM7:TR?= MX3TRW5ITVJ?
T47D M4LweWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYLJR|UxRTRzLkCxPVIh|ryP MWLTRW5ITVJ?
G-402 NGXWWXRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlLaTWM2OD12MT6wPFA6KM7:TR?= NWH3VYo2W0GQR1XS
MZ1-PC MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoH1TWM2OD12MT64NUDPxE1? NIfYfpJUSU6JRWK=
SW1417 MoXPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWPJR|UxRTR{LkOwN{DPxE1? NX3oXpZbW0GQR1XS
RERF-LC-MS MoKxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVPJR|UxRTR{LkWwPFch|ryP NW\uSVJDW0GQR1XS
SF539 NHGxbG9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHy2XVJKSzVyPUSyMlU2OjlizszN MmrVV2FPT0WU
ESS-1 M3e0[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUG3[mJyUUN3ME20N{42PTV6IN88US=> NXWwNI9uW0GQR1XS
RO82-W-1 NXXreJd{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2XhbGlEPTB;NEOuOlE2OyEQvF2= MmXVV2FPT0WU
S-117 NIjSfoNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlfRTWM2OD12Mz62PVU5KM7:TR?= M4\XVHNCVkeHUh?=
SW756 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX;LRm1LUUN3ME20N{45OjR{IN88US=> M4Xz[nNCVkeHUh?=
CP66-MEL M2L0eGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4j3cmlEPTB;NEWuO|E1OyEQvF2= NHfwVG9USU6JRWK=
H-EMC-SS NF\IRpRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGrDPVBKSzVyPUS1Mlc3QTNizszN MV;TRW5ITVJ?
NCI-H2052 MnvES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NY\iZYw2UUN3ME20OU46ODJ5IN88US=> Mkm3V2FPT0WU
SW684 MmHFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1TD[WlEPTB;NEeuNFA5OSEQvF2= M1H2U3NCVkeHUh?=
HCC1569 MomzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXvKZ281UUN3ME20O{4yPjV6IN88US=> MUfTRW5ITVJ?
KGN NE[5fmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4nSTmlEPTB;NEeuN|MyOSEQvF2= NIrORY5USU6JRWK=
LCLC-103H NHf1SJVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkfMTWM2OD12Nz60OFA1KM7:TR?= NF7oVVFUSU6JRWK=
SW780 MmrFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUfJR|UxRTR5Lk[zOVEh|ryP MYjTRW5ITVJ?
NCI-H1703 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1frZmlEPTB;NEiuN|M2OiEQvF2= M{K4fnNCVkeHUh?=
HCC1395 NHTadlFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYTJR|UxRTR6Lke0N|Ih|ryP M{fBcXNCVkeHUh?=

... Click to View More Cell Line Experimental Data

In vivo Gefitinib (100 mg/kg) improves the anti-tumor effect of radiotherapy in LoVo tumor xenografts. [3] Gefitinib treatment of nude mice bearing established human GEO colon cancer xenografts reveals a reversible dose-dependent inhibition of tumor growth because GEO tumors resumes the growth rate of controls at the end of the treatment. [4]

Protocol

Cell Research:[1]
+ Expand
  • Cell lines: NR6, NR6M and NR6W cells
  • Concentrations: 0-2 μM
  • Incubation Time: 72 hours
  • Method: Exponentially growing cells including NR6, NR6M, NR6M and NR6W cells are seeded in sextuple in 96-well plates at a concentration of 2000 cells/well, allowed to adhere and subsequently washed in PBS and incubated overnight in medium containing 0.5% FCS. Cells are then treated with varying concentrations (0-2 μM) of Gefitinib or the solute control DMSO and EGF. The optimal EGF concentration for inducing proliferation of NR6wtEGFR and NR6W cells has previously been determined and hence NR6wtEGFR and NR6W cells are supplemented with 10 nM and 0.1 nM EGF, respectively. EGF is not added to NR6 and NR6M cells. After 72 hours the amount of cells are measured by performing a MTT proliferation assay.
    (Only for Reference)
Animal Research:[3]
+ Expand
  • Animal Models: Female nude mice (cba nu/nu) aged 8–10 weeks are intra-dermal injected with LoVo cells.
  • Formulation: 0.5% polysorbate
  • Dosages: 100 mg/kg
  • Administration: Once daily by oral administration (0.1 mL/10 g body weight) for 14 days
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 89 mg/mL (199.14 mM)
Ethanol 4 mg/mL (8.95 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
1% CMC Na
For best results, use promptly after mixing.
30mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 446.90
Formula

C22H24ClFN4O3

CAS No. 184475-35-2
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00025675 Completed Brain and Central Nervous System Tumors Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins|National Cancer Institute (NCI) October 9 2001 Phase 2
NCT03050164 Completed Bioequivalence Study Hunan Kelun Pharmaceutical Co. Ltd. September 8 2016 Phase 1
NCT00684385 Completed Non Small Cell Lung Cancer|Cancer of the Head and Neck AstraZeneca August 8 2001 Phase 4
NCT01933347 Active not recruiting Non Small Cell Lung Cancer Guangdong Association of Clinical Trials April 7 2014 Phase 2
NCT02411448 Recruiting Metastatic Non-Small Cell Lung Cancer Eli Lilly and Company May 6 2015 Phase 3
NCT03486496 Not yet recruiting Lung Adenocarcinoma|EGFR Mutation Fujian Cancer Hospital June 5 2018 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    I try to dissolve Gefitinib (ZD1839) in 0.5% methylcellulose+0.2% Tween 80 for in vivo experiments. However, when I prepare the mixture, it forms precipitates. Exists another way to dissolve completely the compound?

  • Answer:

    S1025 can be dissovled in both 5% DMSO+corn oil and 5% DMSO+30% PEG 300+5% Tween 80+ddH2O at 2.5 mg/ml clearly. It dissolves in DMSO not readily, please sonicate and warm it in water bath at about 45 degree, and dissolve it clearly first. Then add other solvent.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID