Gefitinib (ZD1839)

Catalog No.S1025

Gefitinib (ZD1839) Chemical Structure

Molecular Weight(MW): 446.90

Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.

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Cited by 91 Publications

12 Customer Reviews

  • Perturbation of EGFR by its ligand EGF and gefitinib (ZD-1839 Iressat; inhibits EGFR) produces opposite responses in the predicted EGFR target genes SOCS2 and NR2E1.

    Mol Syst Biol 2011 7, 486. Gefitinib (ZD1839) purchased from Selleck.

    Effects of shRNA-mediated RECK depletion on EGFR signaling in MEFs. (a) Immunoblotting (IB) of the indicated proteins in wild-type MEFs transduced with the indicated shRNAs and cultured for 48 h in the presence of 0.1% dimethyl sulfoxide (DMSO; vehicle) or 1 mM gefitinib (S1025, Selleck Chemicals,Houston, TX, USA). (b) Immunoprecipitation from whole lysates of cells from panel a with an anti-phosphorylated tyrosine (pTyr) antibody. Precipitates from 500 mg protein in whole cell lysates (upper) or 20 mg protein in whole cell lysates (lower) were analyzed by IB with anti-EGFR antibody.

    Oncogene 2010 30, 737-750. Gefitinib (ZD1839) purchased from Selleck.

  • (B–C) LNCaP (B) and LNCaP-AI (C) cells were transiently transfected with sPLA2-IIa(-800)-Luc (0.5 μg). The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 lM) and Bortezomib (20 μM) without or with EGF (100 ng/ml) for 24 h. Luciferase assay was performed according to a standard protocol with Renilla luciferase as an internal control. Data are presented as the mean (±SD) of duplicate values of a representative experiment that was independently repeated for five times.

    Carcinogenesis 2010 31, 1948–1955 . Gefitinib (ZD1839) purchased from Selleck.

    LNCaP-AI cells were starved in 1% stripped medium for 24 h. The cells were then treated with Erlotinib (20 μM), Gefitinib (20 μM), Lapatinib (20 μM), CI-1033 (8 μM), LY294002 (20 μM) and Bortezomib (20 μM) for 24 h. Cell culture medium was collected from each sample and subjected to ELISA for sPLA2-IIa. The condition medium samples were diluted 10 times for ELISA. Average of duplicate samples was converted to nanogram per milliliter against standard curve. The data represent one of five repeated experiments.

     

     

    Carcinogenesis 2010 31, 1948–1955. Gefitinib (ZD1839) purchased from Selleck.

  • Susceptibility of lung cancer cells to cytolytic activity of NK-92 cells after treatment with EGFR inhibitors. Three lung cancer cells were untreated (open circle) or treated with 10 μM erlotinib or gefitinib (black filled square or black filled triangle) for 24 hours. The lung cancer cells were cocultured with NK-92 cells at indicated effector to target ratio (E:T ratio). To determine the specificity of NKG2D-mediated cytolysis of lung cancer cells, NK-92 cells were preincubated with blocking mAb against NKG2D before the assay (gray filled square and triangle) and correspond-ing isotype mAb (open square and triangle).

    J Immunother 2011 34(4), 372-81. Gefitinib (ZD1839) purchased from Selleck.

    Suppression of EGFR signaling by Gefitinib is dose dependent and non- toxic at millimolar concentrations.Results from plaque reduction tests of Hep2 cells infected with VACV(12.5pfu/well) treated with a serial dilution of Gefitinib (1000-0.01 m) and analyzed for (A)proliferation as indicator of cytotoxicity,(B) IC50 of plaque size inhibition, (C)dose dependent inhibition of EGFR-ERK1/2signaling and VACV proteins by Western blot analysis (pEGFR detection was obtained from a different experiment with identicals ettings) and (D)dose dependent inhibition of orthopoxvirus genome replication by real-time PCR.Untreated VACV infectedcells and uninfected cells are shown as controls in the right panel.

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

  • A circular zone of EGFR activated but uninfected cells facilitate virus induced plaque development , efficiently blocked by Gefitinib. (A)Immun of luorescent staining of aplaque reduction tests with Hep2 cells 24,48,72 and 96 h after VACV infection (6.25pfu/chamber). VACV infected cells(green) were visualized by human IgG directed to orthopoxviruses (VIG) detected with a secondary FITC labelled antibody. Uninfected cells with activated EGFR signaling(red) are detected with a secondary Cy3labelled antibody. Positive cells for both , VACV and phosphorylated EGFR are shown in yellow(merge). Cellular nuclei are counterstained with DAPI. (B)Immunofluorescent staining of a plaque reduction test as described for (A) and treated with three different Gefitinib concentrations (1000 nM, 1 nM, and 0.01 nM) . The test was analyzed 96h afterVACV infection.Uninfected cells are shown as controls. Magnification100×.(For interpretation of the references to color in this figure legend , the reader is referred to the web version of the article.)

     

     

    Antiviral Res 2010 89, 64-70. Gefitinib (ZD1839) purchased from Selleck.

    The EGF-dependent increased association of PCM1 and AZI1 requires EGFR kinase activity. ZD1839 (Iressa)—EGFR tyrosine kinase inhibitor.

    Mol Biosyst 2011 7, 3223-33. Gefitinib (ZD1839) purchased from Selleck.

  • Reduction of GPER expression in HCC1806 cells after treatment with gefitinib. (A) Concentration dependence. HCC1806 cells were treated with 50-500 nM gefitinib for 48 h. GPER was detected by western blotting. (B) Time dependence. HCC1806 cells were treated with 200 nM gefitinib for 24-96 h. ap<0.05 vs. control; bp<0.001 vs. control. Mean values and SE of three independent experiments are shown. (C) Representative western blotting of concentration dependence. (D) Representative western blotting of time dependence

    Oncol Rep, 2017, 37(2):1212-1218. Gefitinib (ZD1839) purchased from Selleck.

    Inhibition of signaling pathway activation in lung tumor cell lines by kinase inhibitors. Lung tumor cells were cultured in 10% FBS until reaching ∼80% confluence and then the cells were starved in serum-free medium for overnight, followed by 4-hour treatment with the inhibitors. Cell lysates were then prepared and used for determination of the pathway activation signals by the CEER assay.

    Int J Proteomics 2011 215496. Gefitinib (ZD1839) purchased from Selleck.

  • Breast cancer cells were pretreated with 100ng/ml EGF for 15 min and then treated with the indicated concentrations of  Gefitinib for 24 hours.

     

     

    2010 Dr. Zhang of Tianjin Medical University. Gefitinib (ZD1839) purchased from Selleck.

    MTT assay result. 0-500nM Gefitinib treated with H1299, H358, H25 and HCC827 cell lines by 72h, Proliferation rate was tested.

     

     

    2010 Dr. Vicky Tin from University of Hong Kong. Gefitinib (ZD1839) purchased from Selleck.

Purity & Quality Control

Choose Selective EGFR Inhibitors

Biological Activity

Description Gefitinib (ZD-1839) is an EGFR inhibitor for Tyr1173, Tyr992, Tyr1173 and Tyr992 in the NR6wtEGFR and NR6W cells with IC50 of 37 nM, 37nM, 26 nM and 57 nM, respectively.
Features A potent EGFR tyrosine kinase inhibitor.
Targets
Tyr1173 (NR6W cells) [1] Tyr1173 (NR6wtEGFR cells) [1] Tyr992 (NR6wtEGFR cells) [1] Tyr992 (NR6W cells) [1]
26 nM 37 nM 37 nM 57 nM
In vitro

Gefitinib effectively inhibits all tyrosine phosphorylation sites on EGFR in both the high and low-EGFR-expressing cell lines including NR6, NR6M and NR6W cell lines. The phosphorylation sites Tyr1173 and Tyr992 are less sensitive requiring higher concentrations of Gefitinib for inhibition. Gefitinib effectively blocks the phosphorylation of PLC-γ, with IC50 of 27nM, in NR6W cells. The NR6wtEGFR and NR6M cell lines has low levels of PLC-γ phosphorylation but the level in the NR6M cell line is more resistant to inhibition by Gefitinib with IC50 of 43 nM and 369 nM, respectively. Gefitinib inhibits Akt phosphorylations, with IC50 of 220 and 263nM, in the low-EGFR- and -EGFRvIII-expressing cell lines, respectively. Gefitinib in the dose range from 0.1 to 0.5μM significantly facilitates, rather than abrogates, colony formation of NR6M cells. However, at a concentration of 2 μM Gefitinib completely blocks NR6M colony formation. Gefitinib rapidly and in a dose-dependent manner inhibits EGFR and ERK phosphorylation up to 72 hours after EGF stimulation in both the high- and low-EGFR-expressing cell lines. [1] Gefitinib is the monolayer growth of these EGF-driven untransformed MCF10A cells with an IC50 of 20 nM. [2] The combination of Gefitinib (0.2 μM and 0.5 μM) with irradiation lead to a significant growth inhibition in LoVo cells, compared with radiation alone.. [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KB M4PmN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M37HSGlEPTB;MD6wOVQh|ryP NWPYWlRDOTZ3MU[0O|M>
NIH3T3 Mn7mSpVv[3Srb36gRZN{[Xl? MUmwMlIh|ryP NW[5TGpPOiCq MlfQSG1UVw>? NF6xdIhKdmirYnn0bY9vKG:oIFXHSkB{fGmvdXzheIVlKGi3bXHuJIVz[kJzIHH1eI9xcG:|cHjvdplt[XSrb36ge4l1cCCLQ{WwJI9nKDBwMEG0OEDPxE1? M3;RVlE3PDhyMki0
COS7 MWHGeY5kfGmxbjDBd5NigQ>? NHLifYE6PiEQvF2= NWXuUGdVUW6qaXLpeIlwdiCxZjDoeY1idiCMQVuyJHY3OTeIIH31eIFvfCCneIDy[ZN{\WR? NYLJOGdFOTdzN{i3NlI>
A549 MX;DfZRwfG:6aXOgRZN{[Xl? NXL0emxtQTZiaB?= NFTwTHJFVVOR NXXDe|F[UUN3ME2xN{42QSEQvF2= M2XaOlE5OzF|OEC3
K562 Mk[4R5l1d3SxeHnjJGF{e2G7 NH\sS5M6PiCq NYjrPJdFTE2VTx?= NGnjXHVKSzVyPUmuN|Yh|ryP MXWxPFMyOzhyNx?=
MDA-MB-231 NVG2d3ZyS3m2b4TvfIlkKEG|c3H5 MWK5OkBp MXvEUXNQ NGnEfVhKSzVyPUO2MlYyKM7:TR?= MWCxPFMyOzhyNx?=
MOLT4 MnXkR5l1d3SxeHnjJGF{e2G7 Ml3rPVYhcA>? NWC1T45DTE2VTx?= MVvJR|UxRTF3LkCyJO69VQ>? M3jIfFE5OzF|OEC3
MCF7 MV7DfZRwfG:6aXOgRZN{[Xl? M2TCVVUxKM7:TR?= MVi3NkBp Mn\tTWM2OD1zMj6wOUDPxE1? M2PId|E5PzdzOEG5
BT474 M3XSOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH7S[XU4OiCq NEHLcVlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFLUOFc1KGOnbHzzJI93\XKneIDy[ZN{cW6pIFXSRoIzKHerdHigSWM2OCCxZjCwMlMh|ryP NIj2boEyQTB{OESyOC=>
HN5 M1ThSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYrnVpk4PzJiaB?= NInnUodCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjOOUBk\WyuczDveoVz\XiycnXzd4lv\yCHR1\SJJdqfGhiRVO1NEBw\iByLkCxJO69VQ>? NX\UeFI2OTlyMki0NlQ>
BA/F3 M{TndWN6fG:2b4jpZ{BCe3OjeR?= NXniXJpqPzJiaB?= MlvIR5l1d3SxeHnjbZR6KGGpYXnud5QhdW:3c3WgRmEwTjNiY3XscJMh\XiycnXzd4lv\yCHR1\SJGw5PTiUIH31eIFvfCC5aYToJGlEPTBib3[gNE4xOTB6IN88US=> NYDabnN4OTl{M{myNlk>
NIH3T3 NVrJN5Z5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4XxOFczKGh? MkHGS5Jwf3SqIHnubIljcXSrb36gc4YhdW:3c3WgUmlJNzOWMzDj[YxteyCneIDy[ZN{cW6pIHXyRlIh\2WwZTD3bZRpKEmFNUCgc4YhOC53NDFOwG0> NHnSVGkyQTZ4NUO3Oy=>
MCF7 MX M{TUTGZ2dmO2aX;uJGF{e2G7 NXLwZ5ZKOzBibXnu NETOfFVKdmirYnn0bY9vKG:oIFLDVnAh\XiycnXzd4VlKHerdHigTWM2OCCxZjCxMlEh|ryP M1TiWlE6QTN{OU[w
A431 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEiydHoyOCEQvF2= M4\1ZlczKGh? NH;lOYpIUTVyPUCuOVE1KM7:TR?= NVLU[lhlOTl7Nkm0OlU>
A431 MmfZT4lv[XOnIFHzd4F6 NFvXWXIxNjFizszN M3O0fFEhcA>? M2TwT2lvcGmkaYTpc44hd2ZiRVfGMYlv\HWlZXSgSWdHWiCyaH;zdIhwenmuYYTpc44> M3m0dVE6QTZ7NE[1
A431 MVfLbY5ie2ViQYPzZZk> M13sWVExOCEQvF2= M4WxdlExKG2rbh?= MX7Qdo9td26pZXSgbY5pcWKrdH;yfUBi[3Srdnn0fUBw\iCHR1[tbY5lfWOnZDDFS2ZTKHCqb4PwbI9zgWyjdHnvci=> NGTWXFIyQTl4OUS2OS=>
Sf9 NH:zNnVMcW6jc3WgRZN{[Xl? MmnNNVAhdWmw M1znbmROW09? NIPPfVZKdmirYnn0bY9vKG:oIFXHSnIh\XiycnXzd4VlKGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhemWlZYD0c5Ih[XW2b4Doc5NxcG:{eXzheIlwdiC5aYToJGlEPTBib3[gNE4xOzNizszN NYTwT4d2OjByNU[0NlU>
NCI-H1975 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4PsSFUh|ryP NE\LZZY4OiCq NFT5RoFKSzVyPUiuNlYh|ryP NH7INoszODF3MU[3NC=>
NCI-H1975 NHT1[lFMcW6jc3WgRZN{[Xl? MnjCNVAh|ryP NV7GW4t6UW6qaXLpeIlwdiCxZjDFS2ZTKGG3dH;wbI9{eGixconsZZRqd25? M{TX[VIxOTVzNkew
NCI-H1975 NE\0UplHfW6ldHnvckBCe3OjeR?= MVyxNEDPxE1? M1j3e2lvcGmkaYTpc44hd2ZiRWLCRlIh[XW2b4Doc5NxcG:{eXzheIlwdg>? M3zLfFIxOTVzNkew
NCI-H1975 NHHpcndHfW6ldHnvckBCe3OjeR?= NVn5NotUPSEQvF2= NH:4Z3M1QCCq NGP4eI9KdmS3Y4Tpc44hd2ZiYYDvdJRwe2m|IHHzd4V{e2WmIHHzJIFkfGm4YYTpc44hd2ZiY3HzdIF{\S1| MmTONlAyPTF4N{C=
A375 NVPlblhGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NE\OOGM1QCCq MWnJR|UxRTJyLkW1JO69VQ>? NUjEWmN7OjB|MES1N|c>
SMMC7721 M4PTUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXe0PEBp NVT4TZNOUUN3ME2zN{4yOiEQvF2= M{nFPVIxOzB2NUO3
DU145 NIjISFREgXSxdH;4bYMhSXO|YYm= NXLGS5V5PSCm M2PESGROW09? NWnGVIVqUUN3ME20Nk4yPyEQvF2= Mk[zNlA1PjZ3NUW=
MIAPaCa2 NYDIcpoxS3m2b4TvfIlkKEG|c3H5 M3TBeFUh\A>? MYjEUXNQ NVPOfXFsUUN3ME21NE43OiEQvF2= M4\XRVIxPDZ4NUW1
PANC1 M1;hZWN6fG:2b4jpZ{BCe3OjeR?= NVHmb4h7PSCm NXPwfZI3TE2VTx?= MljMTWM2OD12MD60NkDPxE1? M3HG[VIxPDZ4NUW1
PC9 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYnPcHBwSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBGT0[ULXTl[olkcWWwdDDoeY1idiCSQ{mgZ4VtdHNid3n0bEBGSzVyIH;mJFAvODF5IN88US=> NXvpPGlSOjF{MEi4NFI>
PC9 MnTWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHfyc5d1cC2{ZYPpd5RidnRiaIXtZY4hWEN7IHPlcIx{KGW6cILld5NqdmdiRVfGVkBGPzR4X1G3OVAwXDd7MF2gcZV1[W62IIfpeIghTUN3MDDv[kA4KM7:TR?= NYL2ZnlHOjF{MEi4NFI>
PC3 MkLIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUKxNEDPxE1? M2G3[FczKGh? Mme5SG1UVw>? Ml62TWM2OD15LkSg{txO MmnGNlE{PTN3NE[=
MDA-MB-468 NFrEToxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFnoNYQyODBizszN NEnHZ3o1QCCq NUWybIpvTE2VTx?= NFzpW3RCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fS=> M1\OTFIyPDJ7NkOy
Plasmodium falciparum M{fONJFJXFNicILv[oltcW6pIH\vdkBqdmirYnn0c5J{KG:oIGDsZZNud2SrdX2g[oFt[2myYYL1cUBxem:uaX\ldoF1cW:w NEXkd4MzOThzN{C0OS=>
NCI-H1993 NGXzZoxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGiwXlU4OiCq NXv5cpM1TE2VTx?= M4W0SGlEPTB;MD6xJO69VQ>? NYj4XGVmOjF7MU[0N|M>
NCI-H292 NI\qSlBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVzR[HJDPzJiaB?= NYHofpNqTE2VTx?= MorYTWM2OD1yLkCyJO69VQ>? NH7I[XozOTlzNkSzNy=>
A431 NX;HWmpRU2mwYYPlJGF{e2G7 NV24UHF6OiCq NYD3fFM{TE2VTx?= NXn4fnlkUW6qaXLpeIlwdiCxZjDFS2ZTKHCqb4PwbI9zgWyjdHnvckBqdiCHR1[td5RqdXWuYYTl[EBpfW2jbjDBOFMyKHerdHigTWM2OCCxZjCwMlAxOSEQvF2= M2fCd|IzOTBzMUOy
MCF7 MnfHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYjHeVVEOTBywrFOwIcwdUx? MkjvOFghcA>? M{fUb2ROW09? NGPkW3lCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgc5ZmemW6cILld5NqdmdiRVfGVkBo\W6nIIfpeIghUUN3MDDv[kAyOi5yNTFOwG0> NVfmV5pLOjJzMUmxN|A>
BT474 MmTIR5l1d3SxeHnjJGF{e2G7 M3;FRlExKM7:TR?= MVi0PEBp MlTzR5l1d3SxeHnjbZR6KGGpYXnud5Qhe2W{dX2td5Rien[nZDDoeY1idiCEVES3OEBk\Wyucx?= NWrvT4NlOjJzM{izNFg>
MCF7 MV7DfZRwfG:6aXOgRZN{[Xl? M1fGcFExKM7:TR?= NH7WU5E1QCCq MUXDfZRwfG:6aXPpeJkh[WejaX7zeEB{\XK3bT3zeIFzfmWmIHj1cYFvKE2FRkegZ4VtdHNib4\ldk1mgHC{ZYPzbY5oKEW{YlKyJINmdGy| M33qRVIzOTN6M{C4
HCC827 NHzFPINMcW6jc3WgRZN{[Xl? M1r4d|AvOTJ3IN88US=> MkW3NlQhcA>? MUXEUXNQ Mo\zTY5pcWKrdHnvckBw\iCHR1\SJIRmdCCHN{S2MWE4PTBibYX0ZY51KFlzME[4JIF2fG:yaH;zdIhwenmuYYTpc44> NYXxPJpIOjJ|M{mzOFI>
HCC827 M1fQVWtqdmG|ZTDBd5NigQ>? MWGwMlEzPSEQvF2= M4XEXlI1KGh? MnPhSG1UVw>? Mnv1TY5pcWKrdHnvckBw\iCHR1\SJIRmdCCHN{S2MWE4PTBibYX0ZY51NW2nZHnheIVlKEW{azDwbI9{eGixconsZZRqd25? MX:yNlM{QTN2Mh?=
HCC827 MV;LbY5ie2ViQYPzZZk> MlXiNE4yOjVizszN NEnod3czPCCq MUDEUXNQ M3HtU2lvcGmkaYTpc44hd2ZiRVfGVkBl\WxiRUe0Ok1CPzVyIH31eIFvfC2vZXTpZZRm\CCDa4SgdIhwe3Cqb4L5cIF1cW:w MlHMNlI{Ozl|NEK=
A431 NWTYUoliU2mwYYPlJGF{e2G7 MlTINVAxKM7:TR?= NV3xeno3OzBibXnu NF\HUXJKdmirYnn0bY9vKG:oIFXHSnIh\GW{aY\l[EBnem:vIHj1cYFvKEF2M{GgZ4VtdCCvZX3idoFv\SCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKHCxbImtS4x2NVS7cjDwbI9{eGixconsZZRqd25id3n0bEBKSzVyIH;mJFEvODhizszN Mke5NlI3QTh5OEK=
H460 NUfNSZlrS3m2b4TvfIlkKEG|c3H5 NX64Soc5PzJiaB?= MlvTTWM2OD13LkW5JO69VQ>? NX22VHFxOjJ5Mke0OFk>
HT-29 NULP[HdTS3m2b4TvfIlkKEG|c3H5 MmLNO|IhcA>? NWHjb3J5UUN3ME2zMlM3KM7:TR?= M4PBXlIzPzJ5NES5
SGC7901 MXjDfZRwfG:6aXOgRZN{[Xl? NFqxfGE4OiCq NHr3ZYVKSzVyPUGwMlI3KM7:TR?= M3:zTlIzPzJ5NES5
A498 M2fsPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVjnVG4xSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBOFk5KGOnbHzzJIV5eHKnc4PpcochTUeIUjDhcoQhW1KFIIfpeIghUUN3MDDv[kA5NjZizszN MnO0NlI5OTh6NEi=
Calu3 MUDDfZRwfG:6aXOgRZN{[Xl? NYnXWWV4OTBizsznM41N NHHmcoM4OiCq MUTJR|UxRTFwNjFOwG0> M4nvUVI{OTF4MU[4
FADU NH3x[Y1EgXSxdH;4bYMhSXO|YYm= NFq5XGMyOCEQvHevcWw> NID6bFY4OiCq MojvTWM2OD1yLkC1JO69VQ>? MXWyN|EyPjF4OB?=
HCC827 NH3od2lEgXSxdH;4bYMhSXO|YYm= NFq2[WoyOCEQvHevcWw> NIPaZo84OiCq MV;JR|UxRTBwMECxOUDPxE1? M{PnSlI{OTF4MU[4
PC9 NIK2XZVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NE\OZW0yOCEQvHevcWw> NWTHN|VbPzJiaB?= MXzJR|UxRTBwMECxOEDPxE1? NHvYXlgzOzFzNkG2PC=>
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JVM-3 NVfrN5RuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX\pOGJHUUN3ME2yMlM1PjR|IN88US=> NIf6XnVUSU6JRWK=
NCI-H2170 NFfyS3JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVnId2VzUUN3ME2yMlM1Pzh|IN88US=> MmD6V2FPT0WU
EW-18 M1nt[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mle4TWM2OD1{LkO1OFcyKM7:TR?= MWnTRW5ITVJ?
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CFPAC-1 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXH1UHVNUUN3ME2yMlQ{QDZ2IN88US=> MWTTRW5ITVJ?
HH MkPGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnLpTWM2OD1{LkS5PFU1KM7:TR?= MmT6V2FPT0WU
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TE-8 M4TqWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{\zfWlEPTB;Mj62N|Y2OyEQvF2= NWDwXmNJW0GQR1XS
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DMS-79 Ml3uS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYnNO4NIUUN3ME2yMlY6Ojh2IN88US=> NVrJbIpFW0GQR1XS
Detroit562 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYXPWHl[UUN3ME2yMlY6QDR3IN88US=> NFK3d2dUSU6JRWK=
HuP-T4 MoHqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2jaS2lEPTB;Mj63NlgyQCEQvF2= M3uyPXNCVkeHUh?=
QIMR-WIL MkHES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2X5OmlEPTB;Mj63OVk2OiEQvF2= MWfTRW5ITVJ?
BB65-RCC NFnYSmtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYqwSnlbUUN3ME2yMlgxPzF|IN88US=> M2q5OnNCVkeHUh?=
PC-3 NInrOGpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoDsTWM2OD1{LkizN|E4KM7:TR?= NV\PSlNkW0GQR1XS
OVCAR-3 NXXpOndTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVPJR|UxRTJwOEW1PVQh|ryP MmHEV2FPT0WU
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HC-1 M13HR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2ezeGlEPTB;Mj65OFAyOiEQvF2= Mlm5V2FPT0WU
NCI-H1693 NGeyUZJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYPJR|UxRTJwOUW4PVch|ryP MkCzV2FPT0WU
DU-4475 NIflcVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVzMbmFkUUN3ME2yMlk6ODV{IN88US=> NWj5S2tVW0GQR1XS
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BCPAP NHLId2xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2TRUGlEPTB;Mz6wPFI6PSEQvF2= MVfTRW5ITVJ?
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HT-1376 NFvUZ4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mm[5TWM2OD1|LkKwOVA3KM7:TR?= NIrwWpRUSU6JRWK=
NCI-H1793 NIDZWFRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{fa[mlEPTB;Mz6yNVA6QCEQvF2= NVLhN|NxW0GQR1XS
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NCI-H727 NXjUTo9wT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX\Re|BUUUN3ME2zMlM5PTh2IN88US=> M1njbHNCVkeHUh?=
LB771-HNC NXfW[2lRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1frRmlEPTB;Mz6zPVEzPiEQvF2= MUDTRW5ITVJ?
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TE-1 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVvJR|UxRTNwNUmxOlkh|ryP Mny3V2FPT0WU
OAW-28 NXn4NYhnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4TZc2lEPTB;Mz61PVQzPiEQvF2= NYi5cmNmW0GQR1XS
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CaR-1 M3rFT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXjJR|UxRTNwNkGwOFgh|ryP M4rXZ3NCVkeHUh?=
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SK-MES-1 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUTTdFBUUUN3ME2zMlY1PjF2IN88US=> MXXTRW5ITVJ?
PANC-10-05 M1zZVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYT4VHNjUUN3ME2zMlczODd{IN88US=> NYLDdWprW0GQR1XS
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NCI-H28 M3fLXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXTJR|UxRTNwOEK5OlEh|ryP NFf4PZFUSU6JRWK=
NCI-H1155 NGDjWZJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX7u[ll4UUN3ME2zMlg1OTV7IN88US=> M4fUbnNCVkeHUh?=
KURAMOCHI M1vGfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGO4W|JKSzVyPUOuPFYxPTZizszN M2fJ[HNCVkeHUh?=
NCI-H69 NGr2XoRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH\LNItKSzVyPUOuPVAyPzhizszN MVjTRW5ITVJ?
IST-MES1 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF3pb21KSzVyPUOuPVQxOjlizszN NIPiO3dUSU6JRWK=
NH-12 Mn[3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIL0N|dKSzVyPUOuPVQyQDdizszN M3fnS3NCVkeHUh?=
JVM-2 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX7JR|UxRTNwOUS3OVIh|ryP NH7sXGlUSU6JRWK=
EW-16 NUHS[m5[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHm5fpFKSzVyPUOuPVU2PzdizszN NGHXZWRUSU6JRWK=
NCI-H441 NU\lbJFkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3PIR2lEPTB;Mz65O|AyPCEQvF2= Mny1V2FPT0WU
RCC10RGB MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4fYZ2lEPTB;ND6wNVI1PiEQvF2= MmXLV2FPT0WU
TCCSUP M1PNdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVXJR|UxRTRwMUGwOUDPxE1? NX6yPZUxW0GQR1XS
HL-60 NGDlfVFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NInzNYpKSzVyPUSuNVIzPDFizszN NUTPbo14W0GQR1XS
EW-11 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3P1VWlEPTB;ND6xN|I3OSEQvF2= MXrTRW5ITVJ?
KARPAS-299 M3Xrc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH6zbIdKSzVyPUSuN|ExOTNizszN MX3TRW5ITVJ?
SNU-C2B NG\pZXBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NU\QenVlUUN3ME20MlM1Pjh4IN88US=> MYfTRW5ITVJ?
LU-139 NGPzcXdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NE\tUlVKSzVyPUSuN|czPDdizszN Ml\mV2FPT0WU
HCC1937 NHHuUFVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGnkOZpKSzVyPUSuN|c6QTNizszN NHTjZXVUSU6JRWK=
GR-ST MkfMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3q1XGlEPTB;ND60N|E5KM7:TR?= M{TqbHNCVkeHUh?=
SK-OV-3 NED6PJRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlTXTWM2OD12LkS2NFQzKM7:TR?= MnHXV2FPT0WU
A172 Mlq4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHPRbGxKSzVyPUSuOVU2OjFizszN NWLsTFU{W0GQR1XS
NB10 M1LKdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXzJR|UxRTRwNUm3PFEh|ryP M1e2d3NCVkeHUh?=
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KYSE-520 NGi2WnhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYjJR|UxRTRwOEiwNVgh|ryP M3;oOnNCVkeHUh?=
KNS-62 NIj6T2VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4DkdGlEPTB;ND65NFgxPyEQvF2= MoXnV2FPT0WU
ES1 NXzNW5hTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVXuRYdtUUN3ME20Mlk3ODN3IN88US=> MmHSV2FPT0WU
KOSC-2 Ml\SS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mk\kTWM2OD12Lkm5PVU6KM7:TR?= NYDmXmt{W0GQR1XS
TE-9 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1PzVGlEPTB;NT6wNVUzPSEQvF2= NGPPV2tUSU6JRWK=
NCI-H1838 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXXJR|UxRTVwMEKwO|Yh|ryP NFLLNFZUSU6JRWK=
KP-N-YN NIPiWWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYjJR|UxRTVwMUK1PVEh|ryP M{HEd3NCVkeHUh?=
ALL-PO NILyXoVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX\jeZVoUUN3ME21MlIzPTh5IN88US=> MnLvV2FPT0WU
NOMO-1 M4LFXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWjZTm9lUUN3ME21MlI{OTd4IN88US=> MkfRV2FPT0WU
CGTH-W-1 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV25U48zUUN3ME21MlM5PTh2IN88US=> NVfHdHZbW0GQR1XS
ATN-1 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWLJR|UxRTVwNEGxPFQh|ryP NXrVXFBrW0GQR1XS
AGS NFPF[pFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4\mN2lEPTB;NT60PVYyQCEQvF2= M4r3eHNCVkeHUh?=
SW13 NHr2ZZNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYrJR|UxRTVwNUOyOlUh|ryP MXLTRW5ITVJ?
SW1990 NUH4R4xJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn7QTWM2OD13Lk[yNlg2KM7:TR?= MkO4V2FPT0WU
BFTC-905 MnzMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkXtTWM2OD13Lk[1PFc5KM7:TR?= M{\ZRnNCVkeHUh?=
RMG-I MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MojLTWM2OD13Lk[1PVQzKM7:TR?= MmLsV2FPT0WU
HD-MY-Z NEXMRmdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2SzeGlEPTB;NT62PFU5OSEQvF2= NUDDb3ZTW0GQR1XS
HCC1187 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1HTVGlEPTB;NT63NVA{OiEQvF2= Mm[1V2FPT0WU
HCC2998 Mnn6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2jhemlEPTB;NT63NVg4PyEQvF2= MVLTRW5ITVJ?
RT-112 NX2wOG4zT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVvJR|UxRTVwOUCyOFUh|ryP MUPTRW5ITVJ?
CAKI-1 MmjyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEXQNlJKSzVyPU[uNFA6QDVizszN MkG5V2FPT0WU
HDLM-2 NWm1UVBjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXPJR|UxRTZwMEG0PFkh|ryP M{DSRXNCVkeHUh?=
TE-10 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWrJXVFtUUN3ME22MlA1QDN|IN88US=> NVTxVZh{W0GQR1XS
KE-37 MojtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWXJR|UxRTZwMEeyOVgh|ryP MlPsV2FPT0WU
RCM-1 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUPDbIVDUUN3ME22MlA4PzF7IN88US=> NF3vVnJUSU6JRWK=
Mewo MoXFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXHO[IxiUUN3ME22MlEyPDh5IN88US=> MoHYV2FPT0WU
L-428 MoSzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2TOdGlEPTB;Nj6xPFA{PyEQvF2= NGfQOmNUSU6JRWK=
8-MG-BA MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MULJR|UxRTZwMUmzO|kh|ryP M1TNRXNCVkeHUh?=
SK-MEL-28 M2\xeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnPxTWM2OD14LkKwPVY4KM7:TR?= MXrTRW5ITVJ?
VA-ES-BJ MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFTjZY1KSzVyPU[uN|A1PjJizszN M4PacnNCVkeHUh?=
CAL-120 NWXmbI9zT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkXLTWM2OD14LkS1NVYzKM7:TR?= Mkm3V2FPT0WU
GAMG NInhNnhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVjw[IRsUUN3ME22MlUxPjJizszN M2PYUHNCVkeHUh?=
MSTO-221H NHzjPWdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVrJR|UxRTZwNUGxO{DPxE1? NVrOVlQ2W0GQR1XS
GCT NWGyeYFLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV7JR|UxRTZwNUOzPFch|ryP NFPKS25USU6JRWK=
SH-4 NF;WRnVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NULETIJpUUN3ME22MlU5QDR2IN88US=> NIn4cGlUSU6JRWK=
Calu-3 M{LBOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnLRTWM2OD14Lk[1OVY5KM7:TR?= MmPEV2FPT0WU
SK-HEP-1 MlfyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGTne|ZKSzVyPU[uO|E4PTNizszN MkHJV2FPT0WU
PFSK-1 NG[4Z2hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NW\jUHhEUUN3ME22Mlc{PDd6IN88US=> M2n6PHNCVkeHUh?=
NB12 Mk\4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHjN[JVKSzVyPU[uO|g1ODRizszN NXGwT3h5W0GQR1XS
A388 Mkj3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUDJR|UxRTZwOEm1NkDPxE1? MkPDV2FPT0WU
KLE M4C4cGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnzLTWM2OD14Lkm2OVE3KM7:TR?= M4HoRnNCVkeHUh?=
TE-11 MoHNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHWx[lVKSzVyPUeuNFQ6PjdizszN NVvBSZp1W0GQR1XS
NCI-H2405 NG\3fYFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXX3UHVvUUN3ME23MlA5Ozl|IN88US=> M1rDeXNCVkeHUh?=
OVCAR-4 NF:1WppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MojZTWM2OD15LkG1N|Uh|ryP MUPTRW5ITVJ?
KP-N-YS NGX1blBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmO3TWM2OD15LkG4NVQh|ryP NX3vOmpHW0GQR1XS
NOS-1 MlfzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWTZWW5ZUUN3ME23MlI2QDJ6IN88US=> M{H3XXNCVkeHUh?=
DMS-114 MoHJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NG\RSFZKSzVyPUeuNlk3OzFizszN MXjTRW5ITVJ?
NCI-H2030 NITrOWNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3PNfmlEPTB;Nz6zN|Y6QCEQvF2= NXjHWI5pW0GQR1XS
HEL NGHBdIZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MW\JR|UxRTdwM{e1NlEh|ryP MUfTRW5ITVJ?
CAL-62 MoHMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHPLfJdKSzVyPUeuN|k5PjZizszN MYXTRW5ITVJ?
COLO-668 NIKycVhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NID4VZBKSzVyPUeuOFM6PjdizszN M4XqUHNCVkeHUh?=
SKG-IIIa MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVf4SXhWUUN3ME23MlQ2OzhzIN88US=> NX3hfHpmW0GQR1XS
HCC1419 NYDFb3ZkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFnJZ3dKSzVyPUeuOFY5OzNizszN NYHjem1JW0GQR1XS
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NB14 NWnFcGl7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlmzTWM2OD1|OD60OVQ1KM7:TR?= MYfTRW5ITVJ?
D-423MG MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3S2OGlEPTB;M{iuOFg3QSEQvF2= MWDTRW5ITVJ?
NCI-H510A Mnm1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkjZTWM2OD1|OD62OFUyKM7:TR?= NWSxOmlPW0GQR1XS
NMC-G1 Ml74S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1O0OGlEPTB;M{iuO|IzOiEQvF2= NInqTWlUSU6JRWK=
SW948 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWrJR|UxRTN6Lki5OFkh|ryP MV7TRW5ITVJ?
MFM-223 MnK5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF\NTpBKSzVyPUO4Mlk1OzFizszN NGLs[HhUSU6JRWK=
PAN1 M3i1ZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkCzTWM2OD1|OD65PVE6KM7:TR?= MmjrV2FPT0WU
MZ7-mel NYXx[4t[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3;JOWlEPTB;M{muNVE6QSEQvF2= NVnrdoVuW0GQR1XS
COLO-679 M164Smdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlrGTWM2OD1|OT65O|A5KM7:TR?= NWmwfIRIW0GQR1XS
SW872 M{jSbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYrJR|UxRTRyLkKwN|Eh|ryP MnvnV2FPT0WU
HT-1197 NYDtb3dbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{TxXmlEPTB;NECuNlg3QSEQvF2= M1WyTXNCVkeHUh?=
ES6 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVjydlByUUN3ME20NE41PTV{IN88US=> MmjWV2FPT0WU
SNU-387 M3PMTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFT6eIJKSzVyPUSwMlgzOzhizszN MV3TRW5ITVJ?
T47D NVvxUG1qT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYrJR|UxRTRzLkCxPVIh|ryP M4XDVXNCVkeHUh?=
G-402 NISzUo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFrTbolKSzVyPUSxMlA5ODlizszN NVHQemgyW0GQR1XS
MZ1-PC MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYHJR|UxRTRzLkixJO69VQ>? M{LT[XNCVkeHUh?=
SW1417 M1PwUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXe1[oNyUUN3ME20Nk4{ODNizszN NFPsdIZUSU6JRWK=
RERF-LC-MS Mm\BS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlrYTWM2OD12Mj61NFg4KM7:TR?= NHv5epJUSU6JRWK=
SF539 M1S4S2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUDkUHJIUUN3ME20Nk42PTJ7IN88US=> Mn;oV2FPT0WU
ESS-1 NFvZUIJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{XTPWlEPTB;NEOuOVU2QCEQvF2= NV7h[YhJW0GQR1XS
RO82-W-1 NGnkPYxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX\IdoN{UUN3ME20N{43OTV|IN88US=> M1PQenNCVkeHUh?=
S-117 Ml\FS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NE\5Uo1KSzVyPUSzMlY6PThizszN NVHuPGhJW0GQR1XS
SW756 MlrUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYrJR|UxRTR|LkiyOFIh|ryP NIDBeVlUSU6JRWK=
CP66-MEL NXH5fYhiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX6zdJVlUUN3ME20OU44OTR|IN88US=> M33nNXNCVkeHUh?=
H-EMC-SS NEfYZnJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWHJTIU3UUN3ME20OU44Pjl|IN88US=> MoPFV2FPT0WU
NCI-H2052 Ml:1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmrmTWM2OD12NT65NFI4KM7:TR?= MlzIV2FPT0WU
SW684 NWL2T2tkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHTITVZKSzVyPUS3MlAxQDFizszN M3PKWXNCVkeHUh?=
HCC1569 NEjJ[2tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnXDTWM2OD12Nz6xOlU5KM7:TR?= NVTQbmFzW0GQR1XS
KGN M1\BcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIn3Tm5KSzVyPUS3MlM{OTFizszN NGfzbVVUSU6JRWK=
LCLC-103H NEO4VHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXHGd2c4UUN3ME20O{41PDB2IN88US=> NFXXT|hUSU6JRWK=
SW780 MlPNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUfWTotVUUN3ME20O{43OzVzIN88US=> Mnu1V2FPT0WU
NCI-H1703 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmLBTWM2OD12OD6zN|UzKM7:TR?= NVTlb21tW0GQR1XS
HCC1395 NY\VRWhnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlLjTWM2OD12OD63OFMzKM7:TR?= NFj4dHpUSU6JRWK=

... Click to View More Cell Line Experimental Data

In vivo Gefitinib (100 mg/kg) improves the anti-tumor effect of radiotherapy in LoVo tumor xenografts. [3] Gefitinib treatment of nude mice bearing established human GEO colon cancer xenografts reveals a reversible dose-dependent inhibition of tumor growth because GEO tumors resumes the growth rate of controls at the end of the treatment. [4]

Protocol

Cell Research:[1]
+ Expand
  • Cell lines: NR6, NR6M and NR6W cells
  • Concentrations: 0-2 μM
  • Incubation Time: 72 hours
  • Method: Exponentially growing cells including NR6, NR6M, NR6M and NR6W cells are seeded in sextuple in 96-well plates at a concentration of 2000 cells/well, allowed to adhere and subsequently washed in PBS and incubated overnight in medium containing 0.5% FCS. Cells are then treated with varying concentrations (0-2 μM) of Gefitinib or the solute control DMSO and EGF. The optimal EGF concentration for inducing proliferation of NR6wtEGFR and NR6W cells has previously been determined and hence NR6wtEGFR and NR6W cells are supplemented with 10 nM and 0.1 nM EGF, respectively. EGF is not added to NR6 and NR6M cells. After 72 hours the amount of cells are measured by performing a MTT proliferation assay.
    (Only for Reference)
Animal Research:[3]
+ Expand
  • Animal Models: Female nude mice (cba nu/nu) aged 8–10 weeks are intra-dermal injected with LoVo cells.
  • Formulation: 0.5% polysorbate
  • Dosages: 100 mg/kg
  • Administration: Once daily by oral administration (0.1 mL/10 g body weight) for 14 days
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 89 mg/mL (199.14 mM)
Ethanol 4 mg/mL (8.95 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order:
1% CMC Na
30mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 446.90
Formula

C22H24ClFN4O3

CAS No. 184475-35-2
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
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    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03050164 Completed Bioequivalence Study Hunan Kelun Pharmaceutical Co., Ltd. September 8, 2016 Phase 1
NCT02856893 Not yet recruiting NSCLC European Organisation for Research and Treatment of Cancer - EORTC|AstraZeneca March 2017 Phase 2
NCT02976116 Recruiting NSCLC Hutchison Medipharma Limited December 2016 Phase 2
NCT02951637 Not yet recruiting Lung Adenocarcinoma Shanghai Chest Hospital|Ruijin Hospital|Changhai Hospital December 2016 Phase 2
NCT02930954 Not yet recruiting Non-small-cell Lung Cancer Caicun Zhou|Tongji University November 2016 Phase 2
NCT02929693 Enrolling by invitation Cancer Shanghai University of Traditional Chinese Medicine|Shanghai Chest Hospital|Shanghai Pulmonary Hospital, Shanghai, China October 2016 Phase 3

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    I try to dissolve Gefitinib (ZD1839) in 0.5% methylcellulose+0.2% Tween 80 for in vivo experiments. However, when I prepare the mixture, it forms precipitates. Exists another way to dissolve completely the compound?

  • Answer:

    S1025 can be dissovled in both 5% DMSO+corn oil and 5% DMSO+30% PEG 300+5% Tween 80+ddH2O at 2.5 mg/ml clearly. It dissolves in DMSO not readily, please sonicate and warm it in water bath at about 45 degree, and dissolve it clearly first. Then add other solvent.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID