Ruxolitinib (INCB018424)

Catalog No.S1378

Ruxolitinib (INCB018424) Chemical Structure

Molecular Weight(MW): 306.37

Ruxolitinib (INCB018424) is the first potent, selective, JAK1/2 inhibitor to enter the clinic with IC50 of 3.3 nM/2.8 nM in cell-free assays, >130-fold selectivity for JAK1/2 versus JAK3.

Size Price Stock Quantity  
In DMSO USD 208 In stock
USD 160 In stock
USD 230 In stock
USD 320 In stock
USD 800 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Cited by 38 Publications

8 Customer Reviews

  • a, Phospho- and total STAT1 in MDA-MB-453 cells treated with abemaciclib with or without ruxolitinib for 7 days.

    Nature, 2017, 548(7668):471-475. Ruxolitinib (INCB018424) purchased from Selleck.

    j,k, Gene expression ofMYOCD (j) and ACTA2 ( SMA, k) after applying inhibitors of key components involved in the DDR2 downstream signalling pathway to HSCs cultured within 3D collagen matrix subjected to stretching (ST) (n=3, one-way ANOVA, **P=0.0036, ****P<0.0001). l,m, Expression of SMA was significantly reduced after treatment with related inhibitors in early-stage FμNs. (n=4, one-way ANOVA, ***P=0.001, ****P<0.0001). JAK2-i: INCB018424

    Nature Materials, 2017, 16:1252-1261.. Ruxolitinib (INCB018424) purchased from Selleck.

  • Scratching behavior (I), ear thickness measurement (J), representative H&E histopathology (K), and histology score (L) of vehicle control and Rux-treated mice on day 7, n R 10 mice per group. Scale bars indicate 100 mm. Data are represented as mean ± SEM.

    Cell, 2017, 171(1):217-228. Ruxolitinib (INCB018424) purchased from Selleck.

    STAT3 phosphorylation as determined by phospho flow, mixed lymphocyte reactions containing BALB/c spleen-derived CD4+ T cells co-cultured with or without C57BL/6 BM-derived DC preactivated with 20 ng/mL LPS.

    Blood 2014 123(24), 3832-42. Ruxolitinib (INCB018424) purchased from Selleck.

  • BMDMs were isolated from wild-type mice and incubated in the different concentrations of Ruxolitinib for 1 h before stimulation with 500 U/ml IFN-β for 30 min. Levels of GAPDH as well as total and phospho-Tyr705 STAT3 were determined by immunoblotting.

    J Immunol 2012 189(6), 2784-92. Ruxolitinib (INCB018424) purchased from Selleck.

    Miniscule 10 PFU amount of VA7-EGFP results in productive infection only in CT26LacZ cells whereas the self-limiting infection can be rescued with JAK/STAT inhibitor Ruxolitinib in CT26WT cells counteracting also the effects of exogenous IFNβ (100 U/ml) pre-treatment. Scale bar: 200 um.

    Gene Ther 2014 10.1038/gt.2014.83. Ruxolitinib (INCB018424) purchased from Selleck.

  • INCB018424 administration reverses the protective effects of ALA on ONC retinas. A-C: Representative micrographs (200× magnification) of retinal whole mounts obtained from three groups stained with anti-RNA-binding protein with multiple splicing (Rbpms) antibody (green). Sampling location: 2 mm temporal to the optic disc. Sampling field size: 439 × 330 μm2 (20× objective lens). Scale bar: 50 μm. D: Quantitative analysis of Rbpms-positive cells under different experimental conditions (mean ± standard error of the mean [SEM], n = 6 per group). The average number of Rbpms-positive cells/mm2 was calculated. ONC = optic nerve crush animal; ALA-ONC = alpha lipoic acid (ALA) animal pretreated 1 day before ONC; ALA-ONC+I = ALA animal pretreated 1 day before ONC, followed by INCB018424. *** p<0.001, ** p<0.01 compared to the ONC group, ### p<0.001 compared to the ALA-ONC group at the same time point

    Mol Vis, 2016, 22:1122-1136. Ruxolitinib (INCB018424) purchased from Selleck.

     

    HS578T cells were treated with indicated amount of inhibitor for 18 hr. The cells were lysed by cell lysis buffer (20 mM Tris-Cl (pH 8.0); 0.5 M NaCl; 0.25% Triton X-100; 1 mM EDTA; 1 mM EGTA; 10 mM β-glycerophosphate; 10 mM NaF; 300 µM Na3VO4; 1 mM benzamidine) containing 1 mM DTT and 2 µM PMSF. Western blot analyses were performed using cleared cell lysates resolved on sodium dodecyl sulfate (SDS)-polyacrylamide gels, transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Billerica, MA), and probed with specific antibodies using standard procedures. Chemiluminescence reagent was purchased from  Thermo Scientific (Rockford, IL). Horseradish peroxidase-conjugated secondary antibodies from Sigma (St. Louis, MO).

    Yong Weon Yi Georgetown University. Ruxolitinib (INCB018424) purchased from Selleck.

Purity & Quality Control

Choose Selective JAK Inhibitors

Biological Activity

Description Ruxolitinib (INCB018424) is the first potent, selective, JAK1/2 inhibitor to enter the clinic with IC50 of 3.3 nM/2.8 nM in cell-free assays, >130-fold selectivity for JAK1/2 versus JAK3.
Targets
JAK2 [1]
(Cell-free assay)
JAK1 [1]
(Cell-free assay)
2.8 nM 3.3 nM
In vitro

INCB018424 potently and selectively inhibits JAK2V617F-mediated signaling and proliferation in Ba/F3 cells and HEL cells. INCB018424 markedly increases apoptosis in a dose dependent manner in Ba/F3 cells. INCB018424 (64 nM) results in a doubling of cells with depolarized mitochondria in Ba/F3 cells. INCB018424 inhibits proliferating of erythroid progenitors from normal donors and polycythemia vera patients with IC50 of 407 nM and 223 nM, respectively. INCB018424 demonstrates remarkable potency against erythroid colony formation with IC50 of 67nM. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
TF1 MVzLbY5ie2ViQYPzZZk> MVqyNEBucW5? MljuSG1UVw>? Mli3TY5pcWKrdHnvckBw\iCMQVuyJIlvKGi3bXHuJHRHOSClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJGVRVy2rbnT1Z4VlKFOWQWS1JJBpd3OyaH;yfYxifGmxbjD3bZRpKEmFNUCgc4YhOC5yMUNOwG0> MWWyNlY6QDB6NB?=
TF1 NITrXJRMcW6jc3WgRZN{[Xl? NH\QR2EzOCCvaX6= M3zhVWROW09? NYnWcZlHUW6qaXLpeIlwdiCxZjDKRWsyKGmwIHj1cYFvKFSIMTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEmONj3pcoR2[2WmIGPURXQ{KHCqb4PwbI9zgWyjdHnvckB4cXSqIFnDOVAhd2ZiMD6wNlTPxE1? MWKyNlY6QDB6NB?=
Human T cell MYfLbY5ie2ViQYPzZZk> M{jMTWlvcGmkaYTpc44hd2ZiSlHLN{8yKGmwIHj1cYFvKFRiY3XscJMh\XiycnXzd4lv\yCFREOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDJUFIue3SrbYXsZZRm\CCVVFHUOYEheGixc4Doc5J6dGG2aX;uJJdqfGhiSVO1NEBw\iByLkCyN:69VQ>? NUL2UmJwOjN3NEC2OFg>
Human monocyte Mlz0T4lv[XOnIFHzd4F6 MYDJcohq[mm2aX;uJI9nKEqDS{KgbY4hcHWvYX6gcY9vd2O7dHXzJIV5eHKnc4PpcochS0RzNDDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFfNMWNUTi2|dHnteYxifGWmIGPURXQ2[SCyaH;zdIhwenmuYYTpc44hf2m2aDDJR|UxKG:oIECuNFI3|ryP MoP1NlM2PDB4NEi=
Human monocyte NIDkdGRMcW6jc3WgRZN{[Xl? MUTJcohq[mm2aX;uJI9nKEqDS{KvNUBqdiCqdX3hckBud26xY4n0[ZMh\XiycnXzd4lv\yCFREG0JIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gTWZP\2GvbXGtd5RqdXWuYYTl[EBUXEGWMTDwbI9{eGixconsZZRqd25id3n0bEBKSzVyIH;mJFAvODNzzszN M3vFbVI{PTRyNkS4
HEL NF;aWnZEgXSxdH;4bYMhSXO|YYm= MX:1JO69VQ>? NGTab481QCCq M1KyN2N6fG:2b4jpZ{BqdmSneE2xNk4zLQ>? Mmm1NlU6OzF|NEm=
SET-2 M3rZPWN6fG:2b4jpZ{BCe3OjeR?= MoHhOUDPxE1? MkD5OFghcA>? MWjDfZRwfG:6aXOgbY5l\Xh;MUiuO{U> NGDXXoEzPTl|MUO0PS=>
HT93A NVnBPHBMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mly4N|IxKG6P NGT2UXo2KGR? NYDlUnhTTE2VTx?= MkOzTY5pcWKrdHnvckBw\iCJQ2OtSkBqdmS3Y3XkJIdz[W63bH;jfZRq[yCmaX\m[ZJmdnSrYYTpc44> NWfz[oE3OjV6MEW5OlI>
CMK NWTHN|JFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXzye4FnUW6qaXLpeIlwdiCxZjDDUWsh[2G{conpcochfGinIFrBT|NCPTd{VjDteZRifGmxbjDj[YxtKHC{b3zp[oVz[XSrb36= MUKyOVM2OjF{NB?=
CMK NIrNclNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4nOPWlvcGmkaYTpc44hd2ZiQ13LJINienK7aX7nJJRp\SCMQVuzRVY{TCCvdYTheIlwdiClZXzsJJBzd2yrZnXyZZRqd25id3n0bEBKSzVyIH;mJFAvOTZ|IN88US=> M4fUTFI2OzV{MUK0
CMK MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGLx[5NKdmirYnn0bY9vKG:oIFPNT{Bk[XK{eXnu[{B1cGViV2SgTmFMKGOnbHygdJJwdGmoZYLheIlwdiC5aYToJGlEPTBib3[gNE4xPzVizszN M1fGXFI2OzV{MUK0
NCI-H460 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVLBU2N2TE2VTx?= NYfuV2ExUUN3ME2wMlE{KM7:TR?= M2nvNFI2OjF|Nkew
NCI-H358 M1i3eGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFjsdZhFVVOR NWLxWZBqUUN3ME2wMlEh|ryP MlnzNlUzOTN4N{C=
A549 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVzEUXNQ NXT1TVZEUUN3ME2wMlA1KM7:TR?= MUWyOVIyOzZ5MB?=
A549/DDP NVGyXWFlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1TyfmROW09? MWfJR|UxRTBwMkKg{txO NGL0d4QzPTJzM{[3NC=>
NCI-H1299 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVvzcY17TE2VTx?= NHj2O29KSzVyPUCuNlgh|ryP MYmyOVIyOzZ5MB?=
NCI-H2347 M2XISWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MljKSG1UVw>? NVS0cmFMUUN3ME2wMlE4KM7:TR?= MkDUNlUzOTN4N{C=
A549/DDP Mom5SpVv[3Srb36gRZN{[Xl? NF3jV2o{OCCwTR?= MljPOFghcA>? MnnTSG1UVw>? NHvEVoJFd3ewLYLl[5Vt[XSrb36gc4YhW1SDVEOgdIhwe3Cqb4L5cIF1cW:w MXOyOVIyOzZ5MB?=
NCI-H1299 MmXjSpVv[3Srb36gRZN{[Xl? MmHGN|Ahdk1? MmW4OFghcA>? NGS5THpFVVOR NEDT[IFFd3ewLYLl[5Vt[XSrb36gc4YhW1SDVEOgdIhwe3Cqb4L5cIF1cW:w NH\2OmszPTJzM{[3NC=>
NCI-H2347 M2nqSWZ2dmO2aX;uJGF{e2G7 NIfNUog{OCCwTR?= Mn;3OFghcA>? MU\EUXNQ NGTvW|BF\WO{ZXHz[UBqdiCEY3yyJIV5eHKnc4Ppc44> MljWNlUzOTN4N{C=
A549/DDP MljURZBweHSxc3nzJGF{e2G7 M4X0flMxKG6P Mn[zOFghcA>? NUXocY5FTE2VTx?= MYHJcoR2[3Srb36gc4Yh[XCxcITvd4l{ M1;ZeVI2OjF|Nkew
NCI-H1299 M1PucWFxd3C2b4Ppd{BCe3OjeR?= NUXheXd7OzBibl2= Mne4OFghcA>? MUfEUXNQ NGfae4xKdmS3Y4Tpc44hd2ZiYYDvdJRwe2m| NXTDPGJDOjV{MUO2O|A>
NCI-H2347 NHHsco5CeG:ydH;zbZMhSXO|YYm= M3zPclMxKG6P NV\SPZE{PDhiaB?= NG\EZ49FVVOR NIP2XIFKdmS3Y4Tpc44hd2ZiYYDvdJRwe2m| NEHyT48zPTJzM{[3NC=>
Hep3B MWTGeY5kfGmxbjDBd5NigQ>? NXr5[pZ{OSEQvF2= NFzINoMyPiCq MmO2SG1UVw>? NFXa[IlKdXCjaYLld{B1cGViY3HwZYNqfHlib3[gTWhESS2jc4PvZ4lifGWmIHfwNVMxKG23dHHueJMhfG9iYXP0bZZmKFOWQWSzJJdqfGhiSVO1NEBw\iC-NUCg{txO MmS3NlQ2ODF4OEm=
HepG2 M4\Bc2Z2dmO2aX;uJGF{e2G7 NWD3bmVJOSEQvF2= MofXNVYhcA>? MV\EUXNQ NGLJb2JKdXCjaYLld{B1cGViY3HwZYNqfHlib3[gTWhESS2jc4PvZ4lifGWmIHfwNVMxKG23dHHueJMhfG9ic3nncoFtKHSxIGPURXQ{ M3LoNVI1PTBzNki5
Huh7 NU\kb3djTnWwY4Tpc44hSXO|YYm= MUOxJO69VQ>? NFy3foIyPiCq M3XNXGROW09? NWXOZ|V[UW2yYXny[ZMhfGinIHPhdIFkcXS7IH;mJGlJS0FvYYPzc4Nq[XSnZDDndFE{OCCvdYThcpR{KHSxIIPp[45idCC2bzDTWGFVOw>? M{PoelI1PTBzNki5
BaF3 NYHPVWJSU2mwYYPlJGF{e2G7 MUO4NEBvVQ>? NXHIOnhHPiCq NEKwUXdFVVOR M3O4dXJm\HWlZYOgeIhmKHCqb4PwbI9zgWyjdHnvckBw\sLiU2TBWFUhcW5iSlHLNnY3OTeILX31eIF1\WRiQlHGN{1GWE:UIHPlcIw> NYDRcYJHOjR{M{e3PVE>
DLD-1 M3;VZ2tqdmG|ZTDBd5NigQ>? NWLJ[XJzOjVizszN M{m1b|Q5KGh? Ml7ESG1UVw>? NV\YeIhHUW6qaXLpeIlwdiCxZjDKRWsyKHCqb4PwbI9zgWyjdHnvci=> NVi2d|E4OjRyNUC1OVA>
RKO M1f5fWtqdmG|ZTDBd5NigQ>? NHLsRXQzPSEQvF2= NF\CfFE1QCCq M2\pVWROW09? M3nw[mlvcGmkaYTpc44hd2ZiSlHLNUBxcG:|cHjvdplt[XSrb36= Mnu5NlQxPTB3NUC=
DLD-1 MWnLbY5ie2ViQYPzZZk> MkDGNlUh|ryP NUTqO2E2PDhiaB?= MYTEUXNQ M164emlvcGmkaYTpc44hd2ZiSlHLNkBxcG:|cHjvdplt[XSrb36= NETufWkzPDB3MEW1NC=>
RKO MlnmT4lv[XOnIFHzd4F6 MXWyOUDPxE1? NIDMOHY1QCCq M{PTOWROW09? MWfkc4V{KG6xdDDpcohq[mm2IFrBT|EheGixc4Doc5J6dGG2aX;u NIjpeJYzPDB3MEW1NC=>
DLD-1 MmT4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MY[1NEDPxE1? NVjh[GtwPDhiaB?= M4nzfmROW09? NH;2U2NKSzVyPUG1MlUyKM7:TR?= NFzFOW0zPDB3MEW1NC=>
RKO M{LZVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlLPOVAh|ryP MXe0PEBp NVvKVIV1TE2VTx?= NI\2VIlKSzVyPUG0Mlc3KM7:TR?= M1m3XFI1ODVyNUWw
DLD-1 NHrSOVNCeG:ydH;zbZMhSXO|YYm= MoroNlUh|ryP M4\4SFQ5KGh? MXvEUXNQ Mn\iTY5lfWOnczDhdI9xfG:|aYOgZpkh[WO2aY\heIlv\yClYYPwZZNmKDN? M3rZU|I1ODVyNUWw
RKO NH\JcYdCeG:ydH;zbZMhSXO|YYm= MV6yOUDPxE1? MnjvOFghcA>? MYDEUXNQ NYf0V5JGUW6mdXPld{BieG:ydH;zbZMh[nliYXP0bZZifGmwZzDjZZNx[XOnIEO= Mln4NlQxPTB3NUC=
HuH7 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWm1NEDPxE1? M{HMclQ5KGh? Ml7rSG1UVw>? MWW+PFImKHKnZIXjeIlwdg>? Mn7XNlM6PDF6M{K=
SNU182 MlLUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MknqOVAh|ryP M1\QOFQ5KGh? NYW1Upg{TE2VTx?= M1e4b|43PCVicnXkeYN1cW:w MUKyN|k1OTh|Mh?=
SNU423 M3nSWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXvTOItpPTBizszN NHuwe4Q1QCCq MlyzSG1UVw>? MonwQlgyLSC{ZXT1Z5Rqd25? M2PucFI{QTRzOEOy
HuH7 MWjGeY5kfGmxbjDBd5NigQ>? M4jYOlUxKM7:TR?= M2PDUVI1KGh? MoS5SG1UVw>? MX3Jcohq[mm2aX;uJI9nKFOWQWSxJIFv\CCVVFHUN{BxcG:|cHjvdplt[XSrb36gd4lodmmoaXPhcpRtgQ>? MlnxNlM6PDF6M{K=
SNU182 NXvYOVd7TnWwY4Tpc44hSXO|YYm= NITYWXk2OCEQvF2= MV:yOEBp NXW1V|BHTE2VTx?= NV3kdFZtUW6qaXLpeIlwdiCxZjDTWGFVOSCjbnSgV3RCXDNicHjvd5Bpd3K7bHH0bY9vKHOrZ37p[olk[W62bIm= M2nUO|I{QTRzOEOy
SNU423 MV7GeY5kfGmxbjDBd5NigQ>? NYTlb3ZyPTBizszN NFf3OpEzPCCq MnLUSG1UVw>? NEO2XmlKdmirYnn0bY9vKG:oIGPURXQyKGGwZDDTWGFVOyCyaH;zdIhwenmuYYTpc44he2mpbnnmbYNidnSueR?= NFnIb4UzOzl2MUizNi=>

... Click to View More Cell Line Experimental Data

In vivo INCB018424 (180 mg/kg, orally, twice a day) results in survive rate of greater than 90% by day 22 in a JAK2V617F-driven mouse model. INCB018424 (180 mg/kg, orally, twice a day) markedly reduces splenomegaly and circulating levels of inflammatory cytokines, and preferentially eliminated neoplastic cells, resulting in significantly prolonged survival without myelosuppressive or immunosuppressive effects in a JAK2V617F-driven mouse model. [1] The primary end point is reached in 41.9% of patients in the Ruxolitinib group as compared with 0.7% in the placebo group in the double-blind trial of myelofibrosis. Ruxolitinib results in maintaining of reduction in spleen volume and improvement of 50% or more in the total symptom score. [2] A total of 28% of the patients in the Ruxolitinib (15 mg twice daily) group has at least a 35% reduction in spleen volume at week 48 in patients with myelofibrosis, as compared with 0% in the group receiving the best available therapy. The mean palpable spleen length has decreased by 56% with Ruxolitinib but has increased by 4% with the best available therapy at week 48. Patients in the ruxolitinib group has an improvement in overall quality-of-life measures and a reduction in symptoms associated with myelofibrosis. [3]

Protocol

Kinase Assay:[1]
+ Expand

Binding assay:

Recombinant proteins are expressed using Sf21 cells and baculovirus vectors and purified with affinity chromatography. JAK kinase assays use a homogeneous time-resolved fluorescence assay with the peptide substrate (-EQEDEPEGDYFEWLE). Each enzyme reaction is carried out with Ruxolitinib or control, JAK enzyme, 500 nM peptide, adenosine triphosphate (ATP; 1mM), and 2% dimethyl sulfoxide (DMSO) for 1 hour. The 50% inhibitory concentration (IC50) is calculated as INCB018424 concentration required for inhibition of 50% of the fluorescent signal.
Cell Research:[1]
+ Expand
  • Cell lines: Ba/F3 and HEL cells
  • Concentrations: 3 μM
  • Incubation Time: 48 hours
  • Method: Cells are seeded at 2 × 103/well of white bottom 96-well plates, treated with INCB018424 from DMSO stocks (0.2% final DMSO concentration), and incubated for 48 hours at 37 ℃ with 5% CO2. Viability is measured by cellular ATP determination using the Cell-Titer Glo luciferase reagent or viable cell counting. Values are transformed to percent inhibition relative to vehicle control, and IC50 curves are fitted according to nonlinear regression analysis of the data using PRISM GraphPad.
    (Only for Reference)
Animal Research:[1]
+ Expand
  • Animal Models: JAK2V617F-driven mouse model
  • Formulation: 5% dimethyl acetamide, 0.5% methocellulose
  • Dosages: 180 mg/kg
  • Administration: Oral gavage
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 61 mg/mL (199.1 mM)
Ethanol 61 mg/mL (199.1 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order:
2% DMSO+30% PEG 300+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 306.37
Formula

C17H18N6

CAS No. 941678-49-5
Storage powder
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01712659 Recruiting T Cell Leukemia, Adult|Leukemia, Adult T-Cell|T Cell Leukemia, HTLV I Associated National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) October 3, 2012 Phase 2
NCT02913261 Recruiting Corticosteroid Refractory Acute Graft vs Host Disease Novartis Pharmaceuticals|Novartis February 28, 2017 Phase 3
NCT02973711 Not yet recruiting Leukemia, Chronic Myeloid University of Michigan Cancer Center February 2017 Phase 1|Phase 2
NCT03012230 Not yet recruiting Estrogen Receptor Negative|HER2/Neu Negative|Progesterone Receptor Negative|Stage IV Breast Cancer|Triple-Negative Breast Carcinoma Mayo Clinic|National Cancer Institute (NCI) February 2017 Phase 1
NCT02928978 Not yet recruiting Ductal Carcinoma In Situ|Atypical Lobular Hyperplasia|Atypical Ductal Hyperplasia|Lobular Carcinoma In Situ Julie Nangia|Incyte Corporation|Translational Breast Cancer Research Consortium|Baylor Breast Care Center January 2017 Phase 2
NCT02966353 Not yet recruiting Primary Myelofibrosis (PMF)|Post-Polycythemia Vera-Myelofibrosis (PPV-MF)|Post-Essential Thrombocythemia Myelofibrosis (PET-MF) Novartis Pharmaceuticals|Novartis January 2017 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    What is the difference between S2902 and S1378 which seem to have same structure formula according to the product information?

  • Answer:

    These two chemicals are the two different chiral forms of Ruxolitinib. S2902 S-Ruxolitinib is the S form and S1378 Ruxolitinib is the D form. One of the carbon atoms in this molecule is asymmetric, making the two molecules mirror images of each other. The biological activities of these two molecules can be very different because of the confirmation differences.

  • Question 2:

    How about the half-life of the compound (Ruxolitinib)? How long is the duration of the inhibitory effect on JAK-STAT signaling?

  • Answer:

    The half-life of this compound in body is about 2~3 hours according to previous study. Generally, it is longer in vitro culture medium than in vivo. In paper, Ruxolitinib was also used for 24hours. http://www.bloodjournal.org/cgi/pmidlookup?view=long&pmid=24711661.

JAK Signaling Pathway Map

JAK Inhibitors with Unique Features

Related JAK Products

Tags: buy Ruxolitinib (INCB018424) | Ruxolitinib (INCB018424) supplier | purchase Ruxolitinib (INCB018424) | Ruxolitinib (INCB018424) cost | Ruxolitinib (INCB018424) manufacturer | order Ruxolitinib (INCB018424) | Ruxolitinib (INCB018424) distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID