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Celecoxib Licensed by Pfizer

Celecoxib is a selective COX-2 inhibitor with IC50 of 40 nM.

Catalog No.S1261

: Tel: +1-832-582-8158[email protected]

Celecoxib Chemical Structure
Molecular Weight: 381.37

Validation & Quality Control

Product Citations(1)

Blood, 2011, 118(22), 5891-5900

Quality Control & MSDS

Related Compound Libraries

Celecoxib is available in the following compound libraries:

Cambridge Cancer Compound Library(96-well) Chemical Library (96-well) FDA Approved Drug Library (96-well) Pfizer Licensed Library

Product Information

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Dr. Antonino Maria Sparta demonstrates a breakthrough in leukemia research by utilizing two inhibitors produced by Selleck Chemicals.

Cat.No.	Product Name	Solubility (25°C)
S1261	Celecoxib	<1 mg/mL	76 mg/mL	33 mg/mL
S3043	Rofecoxib (Vioxx)	<1 mg/mL	63 mg/mL	<1 mg/mL
S2903	Lumiracoxib (COX-189)	<1 mg/mL	59 mg/mL	<1 mg/mL
S1328	Etodolac (Lodine)	<1 mg/mL	58 mg/mL	58 mg/mL
S1518	Ibuprofen Lysine (NeoProfen)	71 mg/mL	<1 mg/mL	<1 mg/mL
S1645	Ketoprofen (Actron)	<1 mg/mL	51 mg/mL	51 mg/mL
S1646	Ketorolac (Toradol)	75 mg/mL	75 mg/mL	75 mg/mL
S1626	Naproxen (Aleve)	76 mg/mL	3 mg/mL	15 mg/mL
S1638	Ibuprofen (Advil)	<1 mg/mL	41 mg/mL	41 mg/mL
S1713	Piroxicam (Feldene)	<1 mg/mL	66 mg/mL	1 mg/mL
S1903	Diclofenac	14 mg/mL	64 mg/mL	64 mg/mL
S2531	Asaraldehyde (Asaronaldehyde)	<1 mg/mL	39 mg/mL	16 mg/mL
S2577	Phenacetin	<1 mg/mL	36 mg/mL	36 mg/mL
S1959	Tolfenamic acid	<1 mg/mL	52 mg/mL	<1 mg/mL
S2040	Nimesulide	<1 mg/mL	62 mg/mL	<1 mg/mL
S2047	Lornoxicam (Xefo)	<1 mg/mL	2 mg/mL	<1 mg/mL
S2108	Flunixin meglumin	98 mg/mL	98 mg/mL	8 mg/mL
S3008	Zaltoprofen	<1 mg/mL	60 mg/mL	31 mg/mL
S3023	Bufexamac	<1 mg/mL	45 mg/mL	3 mg/mL
S3200	Triflusal	<1 mg/mL	50 mg/mL	50 mg/mL
S4011	Ampiroxicam	<1 mg/mL	90 mg/mL	<1 mg/mL
S4051	Nabumetone	<1 mg/mL	46 mg/mL	25 mg/mL

Product Description

Biological Activity Protocol Chemical Information Product Citations Tech Support & FAQs

Biological Activity

Description	Celecoxib is a selective COX-2 inhibitor with IC50 of 40 nM.
Targets	COX-2
IC50	40 nM ^[1]
In vitro	Celecoxib shows low sensitivity against COX-1 with IC50 of 15 μM. ^[1] Celecoxib shows an anti-proliferative effect on nasopharyngeal carcinoma (NPC) cell lines including HNE1 and CNE1-LMP1 with IC50 of 32.86 μM and 61.31 μM, respectively. ^[2]
In vivo	Celecoxib exhibits a potent, oral anti-inflammatory activity. Celecoxib reduces acute inflammation in the carrageenan edema assay and chronic inflammation in the adjuvant arthritis model with ED50 of 7.1 mg/kg and 0.37 mg/kg/day, respectively. In addition, Celecoxib also exhibits analgesic activity in the Hargreaves hyperalgesia model with ED50 of 34.5 mg/kg. Besides, Celecoxib produces no acute GI toxicity in rats at doses up to 200 mg/kg and no chronic GI toxicity in rats at doses up to 600 mg/kg/day over 10 days. ^[1] In a C3Hf/KamLaw female mouse model, Celecoxib increases median survival time of 105 days (range, 79-145 days) after 13.5 Gy local thoracic irradiation (LTI) alone to 142 days (range, 94-155 days). ^[3]
Clinical Trials	Celecoxib is currently in Phase II clinical trials in patients with recurrent respiratory papillomatosis.
Features

Protocol(Only for Reference)

Kinase Assay: ^[1]

COX enzyme assay in vitro	Expression of COX protein in insect cells is determined by assessing PG-synthetic capability in homogenates from cells incubated for 3 days with COX-1 or COX-2 baculovirus. Cells expressing COX-1 or COX-2 are homogenized and incubated with arachidonic acid (10 μM). COX activity is determined by monitoring PG production. No COX activity is detected in mock-infected Sf9 cells. Celecoxib are preincubated with crude 1% CHAPS homogenates (2-10 μg of protein) for 10 minutes before addition of arachidonic acid. PGE2 formed is detected by ELISA after 10 minute incubation.

COX enzyme assay in vitro

Expression of COX protein in insect cells is determined by assessing PG-synthetic capability in homogenates from cells incubated for 3 days with COX-1 or COX-2 baculovirus. Cells expressing COX-1 or COX-2 are homogenized and incubated with arachidonic acid (10 μM). COX activity is determined by monitoring PG production. No COX activity is detected in mock-infected Sf9 cells. Celecoxib are preincubated with crude 1% CHAPS homogenates (2-10 μg of protein) for 10 minutes before addition of arachidonic acid. PGE2 formed is detected by ELISA after 10 minute incubation.

Cell Assay: ^[2]

Cell lines	HNE1 and CNE1-LMP1
Concentrations	0-75 μM
Incubation Time	48 hours
Method	The antiproliferative effect of Celecoxib on NPC cells is assessed using an MTT assay. Cells are seeded into 96-well plates and allowed to attach for 24 hours. The cells are then treated with increasing concentrations of Celecoxib (0 to 75 μM) dissolved in DMSO (final concentration ≤0.1%) and incubated for up to 48 hours. After the incubation, 20 μL of MTT dye (5 mg/mL) are added to each well and cells are incubated at 37 °C for 4 hours. After removing the supernatants, the crystals are dissolved in DMSO and the absorbance is measured at 490 nm. The half-maximal inhibitory concentration (IC50) values and the 95% confidence intervals are calculated using probit regression using SPSS 15.0 software. The experiment is performed in triplicate and repeated at least three times.

Animal Study: ^[1]

Animal Models	A 0.1 mL aliquot of a 1% solution of carrageenan in 0.9% sterile saline or 1 mg of Mycobacterium butyricum in 50 μL of mineral oil is administered to the right hind foot pad of male Sprague−Dawley rats.
Formulation	Celecoxib is dissolved in 0.5% methyl cellulose and 0.025% Tween-20.
Dosages	≤200 mg/kg
Administration	Administered via p.o.

References

Chemical Information

Download Celecoxib SDF

Molecular Weight (MW)	381.37
Formula	C₁₇H₁₄F₃N₃O₂S
CAS No.	169590-42-5,184007-95-2, 194044-54-7
Synonyms	Celebrex, Celebra

Solubility (25°C) DMSO 76 mg/mL Water <1 mg/mL Ethanol 33 mg/mL
Storage	2 years -20°CPowder
	2 weeks4°Cin DMSO
	6 months-80°Cin DMSO

Chemical Name	4-(5-p-tolyl-3-(trifluoromethyl)-1H-pyrazol-1-yl)benzenesulfonamide

Molarity Calculator Dilution Calculator Molecular Weight Calculator

Research Area

Product Citations (1)

Diagnostic microRNA profiling in cutaneous T-cell lymphoma (CTCL). [Ralfkiaer U, et al. Blood 2011;118(22), 5891-5900]
PubMed: 21865341

Tech Support & FAQs

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Product Categories

Celecoxib Licensed by Pfizer

Validation & Quality Control

Product Citations(1)

Quality Control & MSDS

Related Compound Libraries

Celecoxib is available in the following compound libraries:

Product Information

Compare COX Inhibitors

Product Description

Biological Activity

Protocol(Only for Reference)

Kinase Assay: ^[1]

Cell Assay: ^[2]

Animal Study: ^[1]

References

Chemical Information

Research Area

Product Citations (1)

Tech Support & FAQs

If you have any other enquiries, please leave a message.

Related COX Inhibitors

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Cat.No.	Product Name	Solubility (25°C)
Cat.No.	Product Name	Water	DMSO	Ethanol
S1261	Celecoxib	<1 mg/mL	76 mg/mL	33 mg/mL
S3043	Rofecoxib (Vioxx)	<1 mg/mL	63 mg/mL	<1 mg/mL
S2903	Lumiracoxib (COX-189)	<1 mg/mL	59 mg/mL	<1 mg/mL
S1328	Etodolac (Lodine)	<1 mg/mL	58 mg/mL	58 mg/mL
S1518	Ibuprofen Lysine (NeoProfen)	71 mg/mL	<1 mg/mL	<1 mg/mL
S1645	Ketoprofen (Actron)	<1 mg/mL	51 mg/mL	51 mg/mL
S1646	Ketorolac (Toradol)	75 mg/mL	75 mg/mL	75 mg/mL
S1626	Naproxen (Aleve)	76 mg/mL	3 mg/mL	15 mg/mL
S1638	Ibuprofen (Advil)	<1 mg/mL	41 mg/mL	41 mg/mL
S1713	Piroxicam (Feldene)	<1 mg/mL	66 mg/mL	1 mg/mL
S1903	Diclofenac	14 mg/mL	64 mg/mL	64 mg/mL
S2531	Asaraldehyde (Asaronaldehyde)	<1 mg/mL	39 mg/mL	16 mg/mL
S2577	Phenacetin	<1 mg/mL	36 mg/mL	36 mg/mL
S1959	Tolfenamic acid	<1 mg/mL	52 mg/mL	<1 mg/mL
S2040	Nimesulide	<1 mg/mL	62 mg/mL	<1 mg/mL
S2047	Lornoxicam (Xefo)	<1 mg/mL	2 mg/mL	<1 mg/mL
S2108	Flunixin meglumin	98 mg/mL	98 mg/mL	8 mg/mL
S3008	Zaltoprofen	<1 mg/mL	60 mg/mL	31 mg/mL
S3023	Bufexamac	<1 mg/mL	45 mg/mL	3 mg/mL
S3200	Triflusal	<1 mg/mL	50 mg/mL	50 mg/mL
S4011	Ampiroxicam	<1 mg/mL	90 mg/mL	<1 mg/mL
S4051	Nabumetone	<1 mg/mL	46 mg/mL	25 mg/mL

Choose Your Country or Region

Product Categories

Celecoxib Licensed by Pfizer

Validation & Quality Control

Product Citations(1)

Quality Control & MSDS

Related Compound Libraries

Celecoxib is available in the following compound libraries:

Product Information

Compare COX Inhibitors

Product Description

Biological Activity

Protocol(Only for Reference)

Kinase Assay: [1]

Cell Assay: [2]

Animal Study: [1]

References

Chemical Information

Research Area

Product Citations (1)

Tech Support & FAQs

If you have any other enquiries, please leave a message.

Related COX Inhibitors

Neuronal Signaling Pathway Products

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Kinase Assay: ^[1]

Cell Assay: ^[2]

Animal Study: ^[1]