Name: Niflumic acid
Brand: Selleck Chemicals
SKU: S3018
Availability: InStock
Rating: 5 (1 reviews)

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Batch: S301801 DMSO]56 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.92%

Cited in Nature Medicine for its top-tier quality
COA
NMR
HPLC
SDS
Datasheet

99.92

Related Targets	Adrenergic Receptor AChR 5-HT Receptor Calcium Channel Histamine Receptor Dopamine Receptor GABA Receptor TRP Channel Cholinesterase (ChE) GluR
Other COX Inhibitors	NS-398 (NS398) Paeoniflorin (NSC 178886) Lumiracoxib Xanthohumol Bufexamac Oroxin B Tolfenamic Acid Nimesulide Ginsenoside Rd Indometacin Sodium

Solubility

In vitro
Batch:

DMSO : 56 mg/mL (198.42 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Chemical Information, Storage & Stability

Molecular Weight	282.22	Formula	C₁₃H₉F₃N₂O₂	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	4394-00-7	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Nifluril			Smiles	C1=CC(=CC(=C1)NC2=C(C=CC=N2)C(=O)O)C(F)(F)F

Mechanism of Action

Targets/IC₅₀/Ki	COX-2 GABA receptor
In vitro	Niflumic acid inhibits Ca²⁺-activated Cl- channels with inhibition constant of 17 mM. This compound also inhibits ICl(Ca) elicited by bath application of Ca²⁺ to oocytes permeabilized using the Ca²⁺ ionophore A23187, demonstrating that the inhibition of ICl(Ca) is due to a direct interaction with the Cl- channel, rather than by interference with Ca²⁺ entry through voltage-dependent Ca²⁺ channels. It blocks Ca²⁺-activated non-selective cation channels in inside-out patches from the basolateral membrane of rat exocrine pancreatic cells with IC50 of 50 μM. This chemical dose-dependently and reversibly activates large conductance calcium-activated K+ (KCa) channels. It produces a concentration-dependent inhibition of spontaneous transient inward current (STIC, calcium-activated chloride current) amplitude. This compound inhibits noradrenaline- and caffeine-evoked IO(Ca) with an ICM50 of 6.6 μM, i.e.is less potent against evoked currents compared to spontaneous currents. It voltage-dependently inhibits spontaneous transient inward current (STIC) amplitude with IC50 of 2.3 μM and 1.1 μM at -50 and +50 mV respectively. It inhibits not only IL-13-induced goblet cell hyperplasia but also airway hyperresponsiveness and eosinophilic infiltration. This chemical suppresses the eotaxin levels in bronchoalveolar lavage fluids and overexpression of the MUC5AC gene, a marker of goblet cell hyperplasia, in the lung after IL-13 instillation. It suppresses JAK2 activation, STAT6 activation, and eotaxin expression in epithelial cells.
References	[1] https://pubmed.ncbi.nlm.nih.gov/1692608/ [2] https://pubmed.ncbi.nlm.nih.gov/1696554/ [3] https://pubmed.ncbi.nlm.nih.gov/7535111/ [4] https://pubmed.ncbi.nlm.nih.gov/16528019/

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Niflumic acid COX inhibitor

Chemical Structure

Molecular Weight: 282.22