Catalog No.S3023

Bufexamac is a COX inhibitor for IFN-α release with EC50 of 8.9 μM.

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In DMSO USD 130 In stock
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Bufexamac Chemical Structure

Bufexamac Chemical Structure
Molecular Weight: 223.27

Validation & Quality Control

Quality Control & MSDS

Related Compound Libraries

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Product Information

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Product Description

Biological Activity

Description Bufexamac is a COX inhibitor for IFN-α release with EC50 of 8.9 μM.
Targets IFN-α
IC50 8.9 μM [1]
In vitro Bufexamac is a specific inhibitor of class IIB histone deacetylases (HDAC6 and HDAC10). Treatment of peripheral blood mononuclear cells with bufexamac inhibits the secretion of IFN-α. [1] Bufexamac is a frequent and relevant contact sensitizer. Bufexamac is a non-steroidal anti-inflammatory drug. [2]
In vivo
Features A specific inhibitor of class IIB histone deacetylases.

Protocol(Only for Reference)

Kinase Assay: [1]

Enzymatic deacetylation assays The enzyme activity of purified recombinant HDAC1, HDAC2, HDAC3-NCoR and HDAC6 (1 μg of protein per well) is measured using the Active Motif HDAC Assay Kit in 96-well format following the manufacturer's instructions. Fluorescence measurements (340 nm excitation/460 nm emission) are recorded with an Analyst HT plate reader in triplicates. Each time series is performed in duplicate. For the determination of enzymatic activity in endogenous HDAC complexes, cell extract samples (375 μL each at 4 mg/mL protein concentration) are prepared from either bufexamac- or vehicle-treated HeLa cultures and are incubated with 15 μg of each antibody for 2 hours at 4 °C. Subsequently, 60 μL Protein G beads equilibrated in lysis buffer containing 0.4% NP40 are added and, following incubation for 1 hour at 4 °C, beads are washed twice with lysis buffer followed by 10 volumes of Assay buffer (HDAC Assay Kit). 10 μL of beads per well is added and deacetylation activity is determined as described above after 150 minutes in the absence or presence of 10 μM trichostatin A. Each point is measured in triplicate. Statistical significance is assessed by unpaired Student's t-test.


Chemical Information

Download Bufexamac SDF
Molecular Weight (MW) 223.27


CAS No. 2438-72-4
Storage 3 years -20℃Powder
6 months-80℃in DMSO
Syonnyms N/A
Solubility (25°C) * In vitro DMSO 45 mg/mL (201 mM)
Water <1 mg/mL (<1 mM)
Ethanol 3 mg/mL (13 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Name 2-(4-butoxyphenyl)-N-hydroxyacetamide

Preparing Stock Solutions

Stock Solution (1ml DMSO) 1mM 10mM 20mM 30mM
Mass(mg) 0.22327 2.2327 4.4654 6.6981

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