Bufexamac

Catalog No.S3023

Bufexamac is a COX inhibitor for IFN-α release with EC50 of 8.9 μM.

Price Stock Quantity  
In DMSO USD 130 In stock
USD 97 In stock
Bulk Inquiry

Massive Discount Available

Free Overnight Delivery on all orders over $ 500.

Bufexamac Chemical Structure

Bufexamac Chemical Structure
Molecular Weight: 223.27

Validation & Quality Control

Quality Control & MSDS

Related Compound Libraries

Bufexamac is available in the following compound libraries:

Product Information

  • Compare COX Inhibitors
    Compare COX Inhibitors
  • Research Area

Product Description

Biological Activity

Description Bufexamac is a COX inhibitor for IFN-α release with EC50 of 8.9 μM.
Targets IFN-α
IC50 8.9 μM [1]
In vitro Bufexamac is a specific inhibitor of class IIB histone deacetylases (HDAC6 and HDAC10). Treatment of peripheral blood mononuclear cells with bufexamac inhibits the secretion of IFN-α. [1] Bufexamac is a frequent and relevant contact sensitizer. Bufexamac is a non-steroidal anti-inflammatory drug. [2]
In vivo
Features A specific inhibitor of class IIB histone deacetylases.

Protocol(Only for Reference)

Kinase Assay: [1]

Enzymatic deacetylation assays The enzyme activity of purified recombinant HDAC1, HDAC2, HDAC3-NCoR and HDAC6 (1 μg of protein per well) is measured using the Active Motif HDAC Assay Kit in 96-well format following the manufacturer's instructions. Fluorescence measurements (340 nm excitation/460 nm emission) are recorded with an Analyst HT plate reader in triplicates. Each time series is performed in duplicate. For the determination of enzymatic activity in endogenous HDAC complexes, cell extract samples (375 μL each at 4 mg/mL protein concentration) are prepared from either bufexamac- or vehicle-treated HeLa cultures and are incubated with 15 μg of each antibody for 2 hours at 4 °C. Subsequently, 60 μL Protein G beads equilibrated in lysis buffer containing 0.4% NP40 are added and, following incubation for 1 hour at 4 °C, beads are washed twice with lysis buffer followed by 10 volumes of Assay buffer (HDAC Assay Kit). 10 μL of beads per well is added and deacetylation activity is determined as described above after 150 minutes in the absence or presence of 10 μM trichostatin A. Each point is measured in triplicate. Statistical significance is assessed by unpaired Student's t-test.
1

References

Chemical Information

Download Bufexamac SDF
Molecular Weight (MW) 223.27
Formula

C12H17NO3

CAS No. 2438-72-4
Storage 3 years -20℃Powder
6 months-80℃in DMSO
Syonnyms N/A
Solubility (25°C) * In vitro DMSO 45 mg/mL (201 mM)
Water <1 mg/mL (<1 mM)
Ethanol 3 mg/mL (13 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Name 2-(4-butoxyphenyl)-N-hydroxyacetamide

Preparing Stock Solutions

Stock Solution (1ml DMSO) 1mM 10mM 20mM 30mM
Mass(mg) 0.22327 2.2327 4.4654 6.6981

Research Area

Tech Support & FAQs

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

* Indicates a Required Field

Related COX Inhibitors

  • Vortioxetine (Lu AA21004) HBr

    Vortioxetine (Lu AA21004) is a multimodal serotonergic agent, inhibits 5-HT1A, 5-HT1B, 5-HT3A, 5-HT7 receptor and SERT with IC50 of 15 nM, 33 nM, 3.7 nM, 19 nM and 1.6 nM, respectively. Phase 3.

    Features:A multimodal antidepressant.

  • (+)-Bicuculline

    (+)-Bicuculline is a competitive antagonist of GABAA receptors with IC50 of 2 μM, also blocks Ca(2+)-activated potassium channels.

  • PRX-08066 Maleic acid

    PRX-08066 is a selective 5-HT2B receptor antagonist with IC50 of 3.4 nM, prevents the severity of pulmonary arterial hypertension in the MCT rat model. Phase 2.

    Features:A selective 5-HT2BR antagonist and high selectivity over the closely related 5-HT2A and 5-HT2C receptors and other receptor targets.

  • Celecoxib

    Celecoxib is a selective COX-2 inhibitor with IC50 of 40 nM.

  • Ibuprofen

    Ibuprofen (Dolgesic) is an anti-inflammatory inhibitor targeting COX-1 and COX-2 with IC50 of 13 μM and 370 μM, respectively.

    Features:Considered a core medicine in the WHO's "WHO Model List of Essential Medicines" (a list of the minimum medical requirements for a basic healthcare system).

  • Rofecoxib

    Rofecoxib is a COX-2 inhibitor with IC50 of 18 nM.

  • Bufexamac

    Bufexamac is a COX inhibitor for IFN-α release with EC50 of 8.9 μM.

    Features:A specific inhibitor of class IIB histone deacetylases.

  • Piroxicam

    Piroxicam is a non-selective COX inhibitor with an IC50 of 6 mM.

  • Etodolac

    Etodolac is a COX inhibitor with an IC50 of 53.5 nM.

  • Ketoprofen

    Ketoprofen is a non-selective NSAID with IC50 of 0.5 μM and 2.33 μM for human recombinant COX-1 and COX-2, respectively.

Recently Viewed Items

Tags: buy Bufexamac | Bufexamac ic50 | Bufexamac price | Bufexamac cost | Bufexamac solubility dmso | Bufexamac purchase | Bufexamac manufacturer | Bufexamac research buy | Bufexamac order | Bufexamac mouse | Bufexamac chemical structure | Bufexamac mw | Bufexamac molecular weight | Bufexamac datasheet | Bufexamac supplier | Bufexamac in vitro | Bufexamac cell line | Bufexamac concentration | Bufexamac nmr
Contact Us