Bufexamac

Catalog No.S3023

Bufexamac Chemical Structure

Molecular Weight(MW): 223.27

Bufexamac is a COX inhibitor for IFN-α release with EC50 of 8.9 μM.

Size Price Stock Quantity  
In DMSO USD 130 In stock
USD 97 In stock
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2 Customer Reviews

  • (a) Treatment of HeLa cells with 1 mM bufexamac results in the induction of HIF1-α, as determined by immunoblotting. The known hypoxia mimetics CPX and DFX were used as positive controls; vinculin served as loading control. (b) Verification of bufexamac-specific upregulation of HIF1-α. HIF1-α protein expression was analyzed 4 h after treatment of cells with the indicated compounds. Ac, acetylated. (c) Time-course analysis of HIF1-α induction by bufexamac. The induction of HIF1-α protein was analyzed by immunoblotting. The graph shows rapid stabilization of HIF1-α upon bufexamac treatment with half-maximal intensity I1/2 of about 75 min. Error bars represent s.d. of three independent experiments. (d) HIF1-α is stabilized only at higher concentrations of bufexamac and accumulates in the nucleus. Induction of endogenous HIF1-α and its nuclear accumulation was analyzed using immunofluorescence microscopy; scale bar, 10 μm.

    Nat Biotechnol, 2015, 33(4):415-23.. Bufexamac purchased from Selleck.

    G. co-IP assay examining the interactions of HDAC10 in 293T cells. Equal amounts of IP products were loaded and subjected to anti-AKT or anti-HDAC10 blotting. The membrane used for anti-HDAC10 blotting (middle panel) was reblotted with anti-p-AKT antibodies (bottom panel). The assay was repeated for three times.

    Oncotarget, 2016, 7(37):59388-59401. Bufexamac purchased from Selleck.

Purity & Quality Control

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Biological Activity

Description Bufexamac is a COX inhibitor for IFN-α release with EC50 of 8.9 μM.
Features A specific inhibitor of class IIB histone deacetylases.
Targets
IFN-α [1]
8.9 μM
In vitro

Bufexamac is a specific inhibitor of class IIB histone deacetylases (HDAC6 and HDAC10). Treatment of peripheral blood mononuclear cells with bufexamac inhibits the secretion of IFN-α. [1] Bufexamac is a frequent and relevant contact sensitizer. Bufexamac is a non-steroidal anti-inflammatory drug. [2]

Protocol

Solubility (25°C)

In vitro DMSO 45 mg/mL (201.54 mM)
Ethanol 3 mg/mL (13.43 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 223.27
Formula

C12H17NO3

CAS No. 2438-72-4
Storage powder
Synonyms N/A

Bio Calculators

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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COX Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID