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Bufexamac

Bufexamac is a COX inhibitor for IFN-α release with EC50 of 8.9 μM.

Catalog No.S3023

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50mg

USD 97

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Bufexamac Chemical Structure
Molecular Weight: 223.27

Validation & Quality Control

Quality Control & MSDS

Related Compound Libraries

Bufexamac is available in the following compound libraries:

Chemical Library (96-well) FDA Approved Drug Library (96-well)

Product Information

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Dr. Antonino Maria Sparta demonstrates a breakthrough in leukemia research by utilizing two inhibitors produced by Selleck Chemicals.

Cat.No.	Product Name	Solubility (25°C)
S1261	Celecoxib	<1 mg/mL	76 mg/mL	33 mg/mL
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S1328	Etodolac (Lodine)	<1 mg/mL	58 mg/mL	58 mg/mL
S1518	Ibuprofen Lysine (NeoProfen)	71 mg/mL	<1 mg/mL	<1 mg/mL
S1645	Ketoprofen (Actron)	<1 mg/mL	51 mg/mL	51 mg/mL
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S1626	Naproxen (Aleve)	76 mg/mL	3 mg/mL	15 mg/mL
S1638	Ibuprofen (Advil)	<1 mg/mL	41 mg/mL	41 mg/mL
S1713	Piroxicam (Feldene)	<1 mg/mL	66 mg/mL	1 mg/mL
S1903	Diclofenac	14 mg/mL	64 mg/mL	64 mg/mL
S2531	Asaraldehyde (Asaronaldehyde)	<1 mg/mL	39 mg/mL	16 mg/mL
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S2040	Nimesulide	<1 mg/mL	62 mg/mL	<1 mg/mL
S2047	Lornoxicam (Xefo)	<1 mg/mL	2 mg/mL	<1 mg/mL
S2108	Flunixin meglumin	98 mg/mL	98 mg/mL	8 mg/mL
S3008	Zaltoprofen	<1 mg/mL	60 mg/mL	31 mg/mL
S3023	Bufexamac	<1 mg/mL	45 mg/mL	3 mg/mL
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Product Description

Biological Activity Protocol Chemical Information Tech Support & FAQs

Biological Activity

Description	Bufexamac is a COX inhibitor for IFN-α release with EC50 of 8.9 μM.
Targets	IFN-α
IC50	8.9 μM ^[1]
In vitro	Bufexamac is a specific inhibitor of class IIB histone deacetylases (HDAC6 and HDAC10). Treatment of peripheral blood mononuclear cells with bufexamac inhibits the secretion of IFN-α. ^[1] Bufexamac is a frequent and relevant contact sensitizer. Bufexamac is a non-steroidal anti-inflammatory drug. ^[2]
In vivo
Clinical Trials
Features	Bufexamac is a specific inhibitor of class IIB histone deacetylases.

Protocol(Only for Reference)

Kinase Assay: ^[1]

Enzymatic deacetylation assays	The enzyme activity of purified recombinant HDAC1, HDAC2, HDAC3-NCoR and HDAC6 (1 μg of protein per well) is measured using the Active Motif HDAC Assay Kit in 96-well format following the manufacturer's instructions. Fluorescence measurements (340 nm excitation/460 nm emission) are recorded with an Analyst HT plate reader in triplicates. Each time series is performed in duplicate. For the determination of enzymatic activity in endogenous HDAC complexes, cell extract samples (375 μL each at 4 mg/mL protein concentration) are prepared from either bufexamac- or vehicle-treated HeLa cultures and are incubated with 15 μg of each antibody for 2 hours at 4 °C. Subsequently, 60 μL Protein G beads equilibrated in lysis buffer containing 0.4% NP40 are added and, following incubation for 1 hour at 4 °C, beads are washed twice with lysis buffer followed by 10 volumes of Assay buffer (HDAC Assay Kit). 10 μL of beads per well is added and deacetylation activity is determined as described above after 150 minutes in the absence or presence of 10 μM trichostatin A. Each point is measured in triplicate. Statistical significance is assessed by unpaired Student's t-test.

Enzymatic deacetylation assays

The enzyme activity of purified recombinant HDAC1, HDAC2, HDAC3-NCoR and HDAC6 (1 μg of protein per well) is measured using the Active Motif HDAC Assay Kit in 96-well format following the manufacturer's instructions. Fluorescence measurements (340 nm excitation/460 nm emission) are recorded with an Analyst HT plate reader in triplicates. Each time series is performed in duplicate. For the determination of enzymatic activity in endogenous HDAC complexes, cell extract samples (375 μL each at 4 mg/mL protein concentration) are prepared from either bufexamac- or vehicle-treated HeLa cultures and are incubated with 15 μg of each antibody for 2 hours at 4 °C. Subsequently, 60 μL Protein G beads equilibrated in lysis buffer containing 0.4% NP40 are added and, following incubation for 1 hour at 4 °C, beads are washed twice with lysis buffer followed by 10 volumes of Assay buffer (HDAC Assay Kit). 10 μL of beads per well is added and deacetylation activity is determined as described above after 150 minutes in the absence or presence of 10 μM trichostatin A. Each point is measured in triplicate. Statistical significance is assessed by unpaired Student's t-test.

References

Chemical Information

Download Bufexamac SDF

Molecular Weight (MW)	223.27
Formula	C₁₂H₁₇NO₃
CAS No.	2438-72-4, 1353882-38-8 (sodium salt)
Synonyms	N/A

Solubility (25°C) DMSO 45 mg/mL Water <1 mg/mL Ethanol 3 mg/mL
Storage	2 years -20°CPowder
	2 weeks4°Cin DMSO
	6 months-80°Cin DMSO

Chemical Name	2-(4-butoxyphenyl)-N-hydroxyacetamide

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Tech Support & FAQs

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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