NS-398 (NS398)

Catalog No.S8433 Synonyms: N-(2-cyclohexyloxy-4-nitrophenyl)methane sulfonamide

NS-398 (NS398) Chemical Structure

Molecular Weight(MW): 314.36

NS-398 is a selective inhibitor of cyclooxygenase-2 (COX-2). The IC50 values for human recombinant COX-1 and -2 are 75 and 1.77 μM, respectively.

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  • (C)Percentage of TUNEL-positive cell in B were calculated. Data are mean ± SEM; ** P < 0.01 versus the Sham group; # P < 0.05 versus the Vehicle group; unpaired two-tailed Student’s t-test. n = 5 per group. (D-G) ELISA of cytokines IL-1α (D), IL-1β (E), IL-6 (F), and TNF-α (G) in tSCI rats with or without NS-398 treatment. Data are mean ± SEM; * P < 0.05 or ** P < 0.01 versus the Sham group; # P <0.05 versus the Vehicle group; unpaired two-tailed Student’s t-test. n = 5 per group. (H) The mean CBS score in tSCI rats was improved by NS-398 treatment. Data are mean ± SEM; ** P < 0.05 versus the Sham group; # P < 0.05 versus the Vehicle group; unpaired two-tailed Student’s t-test. n = 5 per group.

    Oncotarget, 2017, 8(50): 87658-87666. NS-398 (NS398) purchased from Selleck.

Purity & Quality Control

Choose Selective COX Inhibitors

Biological Activity

Description NS-398 is a selective inhibitor of cyclooxygenase-2 (COX-2). The IC50 values for human recombinant COX-1 and -2 are 75 and 1.77 μM, respectively.
Targets
COX-2 [1]
(Cell-free assay)
3.8 μM
In vitro

NS-398 inhibits COX-2 enzyme activity in a concentration dependent manner, the IC50 being 3.8 μM, whereas NS-398 at 100μM has no effect on COX-1 activity[1]. At 10 μM, NS-398 treatment results in increased production of COX-2 and the pro-inflammatory cytokine. NS-398 (10 μM) induces apoptosis in LNCaP cells, but not in the more aggressive, androgen-unresponsive C4-2b cells. The C4-2b cells are observed to continue to proliferate when treated with NS-398 and continues to retain malignant phenotype characteristics. NS-398 treatment results in C4-2b cell differentiation into an unusual neuroendocrinelike cell. These neuroendocrine-like cells produces both epithelial (cytokeratin 18 and prostate specific antigen) and neuronal (neuron-specific enolase and chromogranin A) proteins. Furthermore, this C4-2b cellular response to NS-398 is mediated by NF-kB transcription factor activation. NS-398 induces NF-kB and down-regulates Ikβ-α protein expression in LNCaP C4-2b cells[2].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
RAW264.7 cells MkXLSpVv[3Srb36gZZN{[Xl? MUDFeoFtfWG2ZXSg[o9zKGmwaHnibZRqd25ib3[gR29ZNTJiY3H0ZYx6gmWmIGDHSU0zKHC{b3T1Z5Rqd25iZoLvcUBNWFNiaX7keYNm\CCUQWegNlY1NjdiY3XscJMtKEmFNUC9NE42KM7:TR?= NYrzZnVXOTR7OEC2OVc>
MDA-MB-231 cell MUPGeY5kfGmxbjDhd5NigQ>? MkTzVoVlfWO2aX;uJIlvKFCJRUKgcIV3\Wy|IHnuJG1FSS2PQj2yN|Eh[2WubB?= NVfjfYRQOTZ2OECyO|c>
SKBr3 cells NUOzepZZTnWwY4Tpc44h[XO|YYm= MlPnNlQhcA>? M2jCNXJm\HWldHnvckBqdiCFWWCxPUBuWk6DIHX4dJJme3Orb36gbY4hW0uEckOgZ4VtdHNiYX\0[ZIhOjSqIHX4dI9{fXKnIITvJFI2fU1icnXsZZRqfmVidH:gZ49vfHKxbB?= MlfzNVY1QDB{N{e=
SK-BR-3 cells MX7DfZRwfG:6aXPpeJkh[XO|YYm= NUTDWZhUOjRiaB?= NF7VV|FEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUUy2EUj2zJINmdGy|IHHmeIVzKDJ2IHjyd{BjgSCPVGSgZZN{[XlicnXsZZRqfmVidH:gUnM{QThuIFnDOVA:OC55MjFOwG0> MnzMNVcxQTV{MkG=
SKBR3 cells NFPmb4tHfW6ldHnvckBie3OjeR?= NUXpNGJiUW6qaXLpeIlwdiCxZjDhdo9u[XSjc3WgbY4hcHWvYX6gV2tDWjNiY3XscJMh[nlidILpeIlifGWmIIfheIVzKHKnbHXhd4Uh[XO|YYmsJGlEPTB;MD62PEDPxE1? MVGxPFI4OTVzOR?=
RAW264.7 cells MnTJSpVv[3Srb36gZZN{[Xl? MlLWRY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDpckBud3W|ZTDSRXczPjRwNzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEySUz3pcoR2[2WmIGDHSVIheHKxZIXjeIlwdiCkeTDFTWEtKEmFNUC9OE45KM7:TR?= MXyyNFAxPDV5Mh?=
PMA-Ion-stimulated human PBL MY\GeY5kfGmxbjDhd5NigQ>? Ml2yOUDPxE1? M{Hvb|IxKGh? NI\zbJlKdmirYnn0bY9vKG:oIFPPXFIh\XiycnXzd4lwdiCrbjDQUWEuUW:wLYP0bY12dGG2ZXSgbJVu[W5iUFLMJIF1KDVidV2gZYZ1\XJiMkCgbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=> NGixVGMzOzB2N{KzNS=>
HUVEC NInubYlHfW6ldHnvckBie3OjeR?= M37OO|E5KGh? M{H5eWFvfGmjbnfpc4dmdmmlIHHjeIl3cXS7IHHnZYlve3RiVlXHSmEue3SrbYXsZZRm\CClYYDpcIxienliZHnm[oVz\W62aXH0bY9vKGmwIFjVWmVEKGGodHXyJFE5KGi{czDifUBu[XS{aXflcEBie3OjeR?= NFXRdnIzOzFzMES3OS=>
HUVEC NGHSNIFHfW6ldHnvckBie3OjeR?= M3j3XlQ5KGh? M2OwU2FvfGmjbnfpc4dmdmmlIHHjeIl3cXS7IHHnZYlve3RiVlXHSmEue3SrbYXsZZRm\CClZXzsJJBzd2yrZnXyZZRqd25iaX6gTHVXTUNiYX\0[ZIhPDhiaILzJIJ6KEK{ZGWgbY5kd3Kyb4LheIlwdiCjc4PhfS=> M3zURlI{OTFyNEe1
RAW264.7 cells NVjkVlBGTnWwY4Tpc44h[XO|YYm= M1\ob|I1KGh? MWTBcpRqcW6obHHtcYF1d3K7IHHjeIl3cXS7IHHnZYlve3RibX;1d4UhWkGZMk[0Mlch[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDMVHMucW6mdXPl[EBRT0V{IIDyc4R2[3Srb36gZYRucW6rc4TldoVlKDFiaIKgdJJqd3JidH:gUHBUNWOqYXzs[Y5o\SCvZXHzeZJm\CCjZoTldkAzPCCqcoOgZpkhTUmDLDDJR|UxRTBwMEC3NFEh|ryP NGTo[JozPDN4MEW2NS=>
HaCaT cells M1flUWZ2dmO2aX;uJIF{e2G7 NIrXSGIzPCCq NGT0[GJKdmirYnn0bY9vKG:oIGDHSVIheHKxZIXjeIlwdiCrbjDoeY1idiCKYVPhWEBk\WyuczDh[pRmeiB{NDDodpMh[nliUlnBMEBKSzVyPUCuNFEh|ryP NXzoRoU3OTV|OEe2OFI>
RAW264.7 cells Mm\DSpVv[3Srb36gZZN{[Xl? Ml7ITY5pcWKrdHnvckBw\iCSR1WyJJBzd2S3Y4Tpc44hcW5iTGDTMYlv\HWlZXSgcY92e2ViUlHXNlY1NjdiY3XscJMtKEmFNUC9NE4xODdizszN NFTKR4czPTR3M{iwNC=>
RAW264.7 cells NVOyRodrTnWwY4Tpc44h[XO|YYm= NGnLZYwyPy1{MDDo NVOwN3l3UW6qaXLpeIlwdiCxZjDJSm4u\2GvbXGvUHBUNWmwZIXj[YQhWEeHMjDwdo9lfWO2aX;uJIlvKG2xdYPlJHJCXzJ4ND63JINmdGy|IHHmeIVzKDF5IITvJFIxKGi{czDifUBGUUFibXX0bI9lNCCLQ{WwQVAvOSEQvF2= NXyyNXoxOjVyMke5N|M>
SK-BR-3 cells NFTPTGVHfW6ldHnvckBie3OjeR?= MnzBTY5pcWKrdHnvckBw\iCFWWC0OVAh[XKxbXH0ZZNmKGGldHn2bZR6KGmwIGPLMWJTNTNiY3XscJMtKEmFNUC9NE43QCEQvF2= NHTVR5oyPjR6MEK3Oy=>
RAW264.7 cells MXTGeY5kfGmxbjDhd5NigQ>? M3zvZWlvcGmkaYTpc44hd2ZiQ1;YNk1u\WSrYYTl[EBRT0V{IIDyc4R2[3Srb36gbY4hVFCVLYP0bY12dGG2ZXSgcY92e2ViUlHXNlY1NjdiY3XscJMh[nliZX76fY1mKGmvbYXuc4F{e2G7LDDJR|UxRTBwMEWg{txO M{naVFE6OjN|NkS2
K562 cells MYrGeY5kfGmxbjDhd5NigQ>? NUDxPFM6PCCmYYnz NX\1WlNzUW6qaXLpeIlwdiCxZjDDU3gzKGmwIHj1cYFvKEt3NkKgZ4VtdHNiYYPz[ZN{\WRiYYOgZoxw[2ujZHWgc4YhSU2OMT3FWG8heHKxdHXpck1l\XCnbnTlcpQh\XK7dHjyc4llKGSrZn\ldoVvfGmjdHnvckBi\nSncjC0JIRigXNiYomgZoVvgmmmaX7lJJN1[WmwaX7nJI1mfGixZB?= NEK4b3QyQTF5MkG0Oi=>
RAW264.7 cells MYDGeY5kfGmxbjDhd5NigQ>? MWLJcohq[mm2aX;uJI9nKEORWEKgbY4hdW:3c3WgVmFYOjZ2LkegZ4VtdHNiYomg[Y57gW2nIHntcZVvd2G|c3H5MEBKSzVyPUCuPFEh|ryP MWiyNFA2PjV2OR?=

... Click to View More Cell Line Experimental Data

In vivo NS398 could inhibit Cox-2 expression induced by acoustic injury and could attenuate noise-induced hearing threshold shifts and cochlear hair cell loss. The inhibition of Cox-2 by NS398 could attenuate Noise-induced hearing loss(NIHL)and related hair cell damage.[3].

Protocol

Cell Research:[2]
+ Expand
  • Cell lines: human prostate carcinoma cell line LNCaP and the LNCaP subline C4-2b
  • Concentrations: 10 μM
  • Incubation Time: 24, 48, 72 h
  • Method: 1×106 cells are plated in six well cluster plates with 2 ml of medium for 24 h. At time point 0, medium was removed, cells were carefully washed with phosphate buffered saline (PBS) and serum free(SF), phenol-red free medium containing 0.5 μg/ml BSA was added. Incubations are continued for an additional 72 h with or without increasing NS-398 concentrations. NS-398 stocks (5 mM) are dissolved in 0.1% dimethylsulfoxide (DMSO). Cells and culture medium are harvested at 24 h time intervals. Dead cells are removed by gentle washing with PBS and cell number determined by direct counting using trypan blue dye exclusion to identify viable cells. DMSO (0.1%) is added to control cultures.
    (Only for Reference)
Animal Research:[3]
+ Expand
  • Animal Models: CD1 mice
  • Formulation: Normal saline
  • Dosages: 20 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 62 mg/mL (197.22 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 314.36
Formula

C13H18N2O5S

CAS No. 123653-11-2
Storage powder
in solvent
Synonyms N-(2-cyclohexyloxy-4-nitrophenyl)methane sulfonamide

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COX Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID