NS-398 (NS398)

Catalog No.S8433 Synonyms: N-(2-cyclohexyloxy-4-nitrophenyl)methane sulfonamide

NS-398 (NS398) Chemical Structure

Molecular Weight(MW): 314.36

NS-398 is a selective inhibitor of cyclooxygenase-2 (COX-2). The IC50 values for human recombinant COX-1 and -2 are 75 and 1.77 μM, respectively.

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Biological Activity

Description NS-398 is a selective inhibitor of cyclooxygenase-2 (COX-2). The IC50 values for human recombinant COX-1 and -2 are 75 and 1.77 μM, respectively.
COX-2 [1]
(Cell-free assay)
3.8 μM
In vitro

NS-398 inhibits COX-2 enzyme activity in a concentration dependent manner, the IC50 being 3.8 μM, whereas NS-398 at 100μM has no effect on COX-1 activity[1]. At 10 μM, NS-398 treatment results in increased production of COX-2 and the pro-inflammatory cytokine. NS-398 (10 μM) induces apoptosis in LNCaP cells, but not in the more aggressive, androgen-unresponsive C4-2b cells. The C4-2b cells are observed to continue to proliferate when treated with NS-398 and continues to retain malignant phenotype characteristics. NS-398 treatment results in C4-2b cell differentiation into an unusual neuroendocrinelike cell. These neuroendocrine-like cells produces both epithelial (cytokeratin 18 and prostate specific antigen) and neuronal (neuron-specific enolase and chromogranin A) proteins. Furthermore, this C4-2b cellular response to NS-398 is mediated by NF-kB transcription factor activation. NS-398 induces NF-kB and down-regulates Ikβ-α protein expression in LNCaP C4-2b cells[2].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
RAW264.7 cells M{LxZWZ2dmO2aX;uJIF{e2G7 M1nm[2V3[Wy3YYTl[EBnd3JiaX7obYJqfGmxbjDv[kBEV1hvMjDjZZRidHm8ZXSgVGdGNTJicILv[JVkfGmxbjDmdo9uKEySUzDpcoR2[2WmIGLBW{AzPjRwNzDj[YxteyxiSVO1NF0xNjVizszN NHvZRoEyPDl6ME[1Oy=>
MDA-MB-231 cell NFHwcXRHfW6ldHnvckBie3OjeR?= NH\XRldT\WS3Y4Tpc44hcW5iUFfFNkBt\X[nbIOgbY4hVUSDLV3CMVI{OSClZXzs MoPWNVY1QDB{N{e=
SK-BR-3 cells M1nxd2N6fG:2b4jpZ4l1gSCjc4PhfS=> NIW1NI4zPCCq M2TMXmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHNMNUKULUOgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KE2WVDDhd5NigSC{ZXzheIl3\SC2bzDOV|M6QCxiSVO1NF0xNjd{IN88US=> NGnkTpUyPzB7NUKyNS=>
SKBR3 cells M2XtR2Z2dmO2aX;uJIF{e2G7 NHTOS|FKdmirYnn0bY9vKG:oIHHyc41ifGG|ZTDpckBpfW2jbjDTT2JTOyClZXzsd{BjgSC2cnn0bYF1\WRid3H0[ZIhemWuZXHz[UBie3OjeTygTWM2OD1yLk[4JO69VQ>? NUjUOHZ[OTh{N{G1NVk>
RAW264.7 cells NHjYNHBHfW6ldHnvckBie3OjeR?= NXXuW|hVSW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBqdiCvb4Xz[UBTSVd{NkSuO{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFzQV{1qdmS3Y3XkJHBITTJicILv[JVkfGmxbjDifUBGUUFuIFnDOVA:PC56IN88US=> Ml3FNlAxODR3N{K=
PMA-Ion-stimulated human PBL NHKwcGtHfW6ldHnvckBie3OjeR?= M4rjelUh|ryP MWGyNEBp MoDzTY5pcWKrdHnvckBw\iCFT2iyJIV5eHKnc4Ppc44hcW5iUF3BMWlwdi2|dHnteYxifGWmIHj1cYFvKFCETDDheEA2KHWPIHHmeIVzKDJyIHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYO= NUPYZpVuOjNyNEeyN|E>
HUVEC NFjMSIZHfW6ldHnvckBie3OjeR?= M{XqXlE5KGh? NI\YdJRCdnSrYX7nbY9o\W6rYzDhZ5Rqfmm2eTDh[4FqdnO2IG\FS2ZCNXO2aX31cIF1\WRiY3HwbYxt[XK7IHTp[oZmemWwdHnheIlwdiCrbjDIWXZGSyCjZoTldkAyQCCqcoOgZpkhdWG2cnnn[Ywh[XO|YYm= NIjYPVAzOzFzMES3OS=>
HUVEC MnSySpVv[3Srb36gZZN{[Xl? NFjiUFA1QCCq Ml6zRY51cWGwZ3nv[4VvcWNiYXP0bZZqfHliYXfhbY5{fCCYRVfGRU1{fGmvdXzheIVlKGOnbHygdJJwdGmoZYLheIlwdiCrbjDIWXZGSyCjZoTldkA1QCCqcoOgZpkhSnKmVTDpcoNwenCxcnH0bY9vKGG|c3H5 MlPGNlMyOTB2N{W=
RAW264.7 cells M2\2[WZ2dmO2aX;uJIF{e2G7 NW\1cVd4OjRiaB?= MoDZRY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDh[4FqdnO2IH3veZNmKFKDV{K2OE44KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gUHBUNWmwZIXj[YQhWEeHMjDwdo9lfWO2aX;uJIFldWmwaYP0[ZJm\CBzIHjyJJBzcW:{IITvJGxRWy2laHHscIVv\2VibXXhd5Vz\WRiYX\0[ZIhOjRiaILzJIJ6KEWLQTygTWM2OD1yLkCwO|AyKM7:TR?= NI[3SYQzPDN4MEW2NS=>
HaCaT cells NUnBVpVMTnWwY4Tpc44h[XO|YYm= NXjBc2JFOjRiaB?= M1XkNGlvcGmkaYTpc44hd2ZiUFfFNkBxem:mdXP0bY9vKGmwIHj1cYFvKEijQ3HUJINmdGy|IHHmeIVzKDJ2IHjyd{BjgSCUSVGsJGlEPTB;MD6wNUDPxE1? MkDaNVU{QDd4NEK=
RAW264.7 cells MkTWSpVv[3Srb36gZZN{[Xl? MXHJcohq[mm2aX;uJI9nKFCJRUKgdJJw\HWldHnvckBqdiCOUGOtbY5lfWOnZDDtc5V{\SCUQWeyOlQvPyClZXzsd{whUUN3ME2wMlAxPyEQvF2= MYeyOVQ2OzhyMB?=
RAW264.7 cells MYHGeY5kfGmxbjDhd5NigQ>? NWLIeIc1OTdvMkCgbC=> MXHJcohq[mm2aX;uJI9nKEmITj3nZY1u[S:OUGOtbY5lfWOnZDDQS2UzKHC{b3T1Z5Rqd25iaX6gcY92e2ViUlHXNlY1NjdiY3XscJMh[W[2ZYKgNVchfG9iMkCgbJJ{KGK7IFXJRUBu\XSqb3SsJGlEPTB;MD6xJO69VQ>? MYWyOVAzPzl|Mx?=
SK-BR-3 cells NF7qSmhHfW6ldHnvckBie3OjeR?= M1;Ib2lvcGmkaYTpc44hd2ZiQ2nQOFUxKGG{b33heIF{\SCjY4Tpeol1gSCrbjDTT{1DWi1|IHPlcIx{NCCLQ{WwQVAvPjhizszN M2\jOVE3PDhyMke3
RAW264.7 cells NE\vZZhHfW6ldHnvckBie3OjeR?= NYDjUVN3UW6qaXLpeIlwdiCxZjDDU3gzNW2nZHnheIVlKFCJRUKgdJJw\HWldHnvckBqdiCOUGOtd5RqdXWuYYTl[EBud3W|ZTDSRXczPjRwNzDj[YxteyCkeTDlcpp6dWViaX3teY5w[XO|YYmsJGlEPTB;MD6wOUDPxE1? NHP6U2YyQTJ|M{[0Oi=>
K562 cells MYfGeY5kfGmxbjDhd5NigQ>? NH3PXmk1KGSjeYO= MVjJcohq[mm2aX;uJI9nKEORWEKgbY4hcHWvYX6gT|U3OiClZXzsd{Bie3Onc4Pl[EBieyCkbH;jb4Fl\SCxZjDBUWwyNUWWTzDwdo91\WmwLXTldIVv\GWwdDDldpl1cHKxaXSg[Iln\mW{ZX70bYF1cW:wIHHmeIVzKDRiZHH5d{BjgSCkZX76bYRqdmVic4ThbY5qdmdibXX0bI9l M4jpdFE6OTd{MUS2
RAW264.7 cells MX7GeY5kfGmxbjDhd5NigQ>? M3TYcWlvcGmkaYTpc44hd2ZiQ1;YNkBqdiCvb4Xz[UBTSVd{NkSuO{Bk\WyuczDifUBmdnq7bXWgbY1ufW6xYYPzZZktKEmFNUC9NE45OSEQvF2= M{P1elIxODV4NUS5

... Click to View More Cell Line Experimental Data

In vivo NS398 could inhibit Cox-2 expression induced by acoustic injury and could attenuate noise-induced hearing threshold shifts and cochlear hair cell loss. The inhibition of Cox-2 by NS398 could attenuate Noise-induced hearing loss(NIHL)and related hair cell damage.[3].


Cell Research:[2]
+ Expand
  • Cell lines: human prostate carcinoma cell line LNCaP and the LNCaP subline C4-2b
  • Concentrations: 10 μM
  • Incubation Time: 24, 48, 72 h
  • Method: 1×106 cells are plated in six well cluster plates with 2 ml of medium for 24 h. At time point 0, medium was removed, cells were carefully washed with phosphate buffered saline (PBS) and serum free(SF), phenol-red free medium containing 0.5 μg/ml BSA was added. Incubations are continued for an additional 72 h with or without increasing NS-398 concentrations. NS-398 stocks (5 mM) are dissolved in 0.1% dimethylsulfoxide (DMSO). Cells and culture medium are harvested at 24 h time intervals. Dead cells are removed by gentle washing with PBS and cell number determined by direct counting using trypan blue dye exclusion to identify viable cells. DMSO (0.1%) is added to control cultures.
    (Only for Reference)
Animal Research:[3]
+ Expand
  • Animal Models: CD1 mice
  • Formulation: Normal saline
  • Dosages: 20 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 62 mg/mL (197.22 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 314.36


CAS No. 123653-11-2
Storage powder
Synonyms N-(2-cyclohexyloxy-4-nitrophenyl)methane sulfonamide

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COX Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID