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Hydroxyurea Ribonucleotide Reductase Inhibitor

Cat.No.S1896

Hydroxyurea is an antineoplastic agent that inhibits DNA synthesis through the inhibition of ribonucleoside diphosphate reductase. Hydroxyurea activates apoptosis and autophagy. Hydroxyurea is used to treat HIV infection.

Hydroxyurea DNA/RNA Synthesis inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 76.05

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Quality Control

Batch: Purity: 99.95%

99.95

Related Targets	HDAC PARP ATM/ATR DNA-PK WRN Topoisomerase PPAR Sirtuin Casein Kinase eIF
Other DNA/RNA Synthesis Inhibitors	CX-5461 (Pidnarulex) SCR7 Favipiravir (T-705) RK-33 EED226 BMH-21 Carmofur Triapine (3-AP) YK-4-279 Halofuginone

Solubility in DMSO or Water

In vitro
Batch:

DMSO : 15 mg/mL (197.23 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 15 mg/mL

Ethanol : Insoluble

The solubility data above are all experimental results (not literature values).

DMSO : 15 mg/mL (197.23 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 15 mg/mL

Ethanol : Insoluble

The solubility data above are all experimental results (not literature values).

DMSO : 15 mg/mL (197.23 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 15 mg/mL

Ethanol : Insoluble

The solubility data above are all experimental results (not literature values).

DMSO : 15 mg/mL (197.23 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 15 mg/mL

Ethanol : Insoluble

The solubility data above are all experimental results (not literature values).

DMSO : 15 mg/mL (197.23 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 15 mg/mL

Ethanol : Insoluble

The solubility data above are all experimental results (not literature values).

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Read more about Hydroxyurea solubility in DMSO or water

Search directly for a cell line, or select a cell line to find matched working concentrations

Read more about Hydroxyurea working concentrations for cell culture treatment

Hydroxyurea is a potent inhibitor of Escherichia coli RNR Ia (42.8 mM), HbF, WBC, Neutrophils, Inflammation, Nitric oxide, Cell adhesion, Platelets, Ribonucleotide phosphatase reductase, Elevated cell counts, Inflammatory cytokines, TNF, IL8, IL1B, IL6, Monocyte subsets, NET formation, Viral DNA synthesis, CD4 T cell proliferation, CD8 T cells. Hydroxyurea exhibits the greatest inhibitory potency toward Escherichia coli RNR Ia (IC50 = 42.8 mM).

Read more about selectivity & specificity

Mechanism of Action

Information	Hydroxyurea is a reversible RNR inhibitor. It affects p53 and mTOR pathways by depleting dNTP pools, inhibiting DNA synthesis.
Read more about Hydroxyurea IC50 for cell-based and cell-free assays
Primary Applications and Mechanism of Action

Read more about Hydroxyurea Mechanism of Action

Chemical Information, Storage & Stability

Molecular Weight	76.05	Formula	CH₄N₂O₂	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	127-07-1	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	NCI-C04831, Hydroxycarbamide,NSC-32065			Smiles	C(=O)(N)NO

Read more about storage & stability

Read more comparative analysis about Hydroxyurea

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

Serial Dilutions

Concentration (start):

Dilution-folds:

Computed Result

C1=C0/X	C1:		LOG(C1):
C2=C1/X	C2:		LOG(C2):
C3=C2/X	C3:		LOG(C3):
C4=C3/X	C4:		LOG(C4):
C5=C4/X	C5:		LOG(C5):
C6=C5/X	C6:		LOG(C6):
C7=C6/X	C7:		LOG(C7):
C8=C7/X	C8:		LOG(C8):