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Tetraethylthiuram disulfide (Disulfiram) ALDH Inhibitor

Cat.No.S1680

Disulfiram is a specific inhibitor of aldehyde-dehydrogenase (ALDH) with IC50 of 0.15 μM and 1.45 μM for hALDH1 and hALDH2, respectively. Disulfiram is used for the treatment of chronic alcoholism by producing an acute sensitivity to alcohol. Disulfiram induces apoptosis. Disulfiram is also an inhibitor of pore formation by gasdermin D (GSDMD).

Tetraethylthiuram disulfide (Disulfiram) Dehydrogenase inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 296.54

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Quality Control

Batch: Purity: 99.91%

99.91

Related Targets	HSP Transferase P450 (e.g. CYP17) PDE phosphatase PPAR Vitamin Carbohydrate Metabolism Mitochondrial Metabolism Casein Kinase
Other Dehydrogenase Inhibitors	Vorasidenib (AG-881) (R)-GNE-140 CPI-613 (Devimistat) Glycyrrhizin (Glycyrrhizic Acid, NSC 167409) Sodium Dichloroacetate (DCA) AGI-6780 Emodin Gossypol Acetate AGI-5198 Brequinar (DUP785)

Solubility in DMSO or Water

In vitro
Batch:

DMSO : 59 mg/mL (198.96 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 59 mg/mL

Water : Insoluble

The solubility data above are all experimental results (not literature values).

DMSO : 59 mg/mL (198.96 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

The solubility data above are all experimental results (not literature values).

DMSO : 59 mg/mL (198.96 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 59 mg/mL

Water : Insoluble

The solubility data above are all experimental results (not literature values).

DMSO : 59 mg/mL (198.96 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 59 mg/mL

Water : Insoluble

The solubility data above are all experimental results (not literature values).

DMSO : 59 mg/mL (198.96 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 59 mg/mL

Water : Insoluble

The solubility data above are all experimental results (not literature values).

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	5.000mg/ml (16.86mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	3.900mg/ml (13.15mM)	Taking the 1 mL working solution as an example, add 50 μL of 78 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	3.333mg/ml (11.24mM)	Taking the 1 mL working solution as an example, add 50 μL of 66.67 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	10%DMSO 1 40%PEG300 2 5%Tween80 3 45%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	4.000mg/ml (13.49mM)	Taking the 1 mL working solution as an example, add 100 μL of 40 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to make it clear; then continue to add 450 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	5.000mg/ml (16.86mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2.500mg/ml (8.43mM)	Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Read more about Tetraethylthiuram disulfide (Disulfiram) solubility in DMSO or water

Search directly for a cell line, or select a cell line to find matched working concentrations

Read more about Tetraethylthiuram disulfide (Disulfiram) working concentrations for cell culture treatment

Tetraethylthiuram disulfide (Disulfiram) is a potent inhibitor of rainbow trout liver S9 fractions (CYP1A-like EROD) (Nominal: 32 ± 8 µM; Unbound: 29 ± 15 µM), rainbow trout liver S9 fractions (CYP3A-like BFCOD) (Nominal: 76 ± 15 µM; Unbound: 69 ± 35 µM), recombinant human ALDH2 protein (1.80 µM), gCK (0.58 µM), SVMP (Echis carinatus venom) (6 µM), Giardia lamblia triosephosphate isomerase C202A mutant (6.6 μM), ALDH1 enzyme (0.15 uM), ALDH2 enzyme (1.45 uM), ALDH1 (about 0.1 μg/mL), MAPK/ERK pathway (62.88 ± 0.01 nmol/L), PI3K/AKT pathway (62.88 ± 0.01 nmol/L), ALDH, DBH, NF-κB, Mitochondrial function, DNA repair pathway, NF-κB (p65), Angiogenesis, MMP, Glutathione, ROS, IL-18, VEGF, TNF, IL-8, MMP-2, MMP-9, Proteasome, SOD, P-glycoprotein, MGMT, DNA methyltransferase, NF-kB, DNA replication, Oxidative stress, Mitochondrial membrane permeabilization, Cancer stem cells, Superoxide dismutase, ICE-1, ALDH1A1, ROS reducing agents, Drug efflux pumps, Cancer stem cell function, Caspase-1, IL-1beta, FDX1, SLC31A1, ATP7B, SDHB, PD-L1, HIF-1A, DLAT, Fe-S cluster proteins, NPL4, MDA, Ubiquitin-proteasome system, Drug resistance, Stemness, Metastasis, Glyceraldehyde-3-phosphate dehydrogenase, Fructose-1,6-diphosphate dehydrogenase, Succinic dehydrogenase, Dopamine-β-hydroxylase, DNA repair pathways, MAPK pathway, ClC-3, 26S proteasome, p97 segregase, ATR kinase, NPL4/p97 complex, RPA-ATRIP-ATR-CHK1 repair pathway, STAT3 signalling pathway, P-gp, Nanog, Oct4, NF-kB signalling, PDL-1, SOD1, GSR, Mitochondrial membrane polarization, Extrinsic apoptosis pathway, GSDMD, POH1, Transcription factors, ATF/CREB, DNA topoisomerases, Type IV collagenase, Proteasome activity, Cell cycle, Tumor metastasis, Immune microenvironment, Cuproplasia, Ubiquitin proteasome activity, NF-kB signaling, Cu2+, ·OH, Copper transport, GPX4, NRF2, ALDH isozymes, JNK, p97 pathway, p97-NPL4-dependent processes, ER stress, HSR, DNA damage, GSCs, CSC, NF-kB-stemness gene pathway, Apoptosis, Autophagy, Cell cycle arrest, ICD, Stem cell properties, CSCs, Hypoxic cells, Stem cell transcription factors, ATP7A, NF-kB pathway, TCR signaling, Bivalent metal ion chelation, Caspase-8, ATOX1, JNK/JUN signaling, CD8+T cells, Gasdermin D, NET formation, NETs, Edema, Innate immune function, IL-1 signaling, PRR signaling, TLR signaling, Cytokine signaling, Viral life cycle, IFN signaling genes, IFN regulatory factors, SARS-CoV-2 infection pathway, Complement cascades, Coagulation cascades, Vasopressin-regulated pathways, Oxytocin pathways, Neutrophil infiltration, Heavily infiltrated lung area, Total number of cells, and Perivascular fibrosis. Tetraethylthiuram disulfide (Disulfiram) exhibits the greatest inhibitory potency toward MAPK/ERK pathway and PI3K/AKT pathway (IC50 = 62.88 nM).

Read more about selectivity & specificity

Mechanism of Action

Information	Disulfiram is a potent ALDH/GSDMD inhibitor. It affects NF-κB and ROS pathways by forming Cu complexes, inhibiting tumor growth.
Read more about Tetraethylthiuram disulfide (Disulfiram) IC50 for cell-based and cell-free assays
Primary Applications and Mechanism of Action

Read more about Tetraethylthiuram disulfide (Disulfiram) Mechanism of Action

Chemical Information, Storage & Stability

Molecular Weight	296.54	Formula	C₁₀H₂₀N₂S₄	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	97-77-8	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	NSC 190940, Tetraethylthiuram disulfide, TETD			Smiles	CCN(CC)C(=S)SSC(=S)N(CC)CC

Read more about storage & stability

Read more comparative analysis about Tetraethylthiuram disulfide (Disulfiram)

Tech Support

Handling Instructions

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Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

Serial Dilutions

Concentration (start):

Dilution-folds:

Computed Result

C1=C0/X	C1:		LOG(C1):
C2=C1/X	C2:		LOG(C2):
C3=C2/X	C3:		LOG(C3):
C4=C3/X	C4:		LOG(C4):
C5=C4/X	C5:		LOG(C5):
C6=C5/X	C6:		LOG(C6):
C7=C6/X	C7:		LOG(C7):
C8=C7/X	C8:		LOG(C8):