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Cisplatin DNA Synthesis inhibitor

Name: Cisplatin
Brand: Selleck Chemicals
SKU: S1166
Availability: InStock
Rating: 5 (839 reviews)

Cat.No.S1166

Cisplatin is an inorganic platinum complex, which is able to inhibit DNA synthesis by conforming DNA adducts in tumor cells. Cisplatin activates ferroptosis and induces autophagy.Solutions are unstable and should be fresh-prepared.DMSO is not recommended to dissolve platinum-based drugs, which can easily lead to drug inactivation.

Chemical Structure

Molecular Weight: 300.05

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Quality Control

Batch: Purity: 99.84%

99.84

Related Targets	HDAC PARP ATM/ATR DNA-PK WRN Topoisomerase PPAR Sirtuin Casein Kinase eIF
Other DNA/RNA Synthesis Inhibitors	CX-5461 (Pidnarulex) SCR7 Favipiravir (T-705) EED226 RK-33 BMH-21 Carmofur Triapine (3-AP) YK-4-279 Halofuginone

Solubility in DMSO or Water

In vitro
Batch:

DMF : 15 mg/mL

Water : Insoluble

Ethanol : Insoluble

The solubility data above are all experimental results (not literature values).

DMF : 2.5 mg/mL

Water : 2 mg/mL

Ethanol : Insoluble

The solubility data above are all experimental results (not literature values).

DMF : 10 mg/mL

Water : 1 mg/mL

Ethanol : Insoluble

The solubility data above are all experimental results (not literature values).

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Clear solution	5%DMF 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.750mg/ml (2.50mM)	Taking the 1 mL working solution as an example, add 50 μL 15 mg/ml clarified DMF stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMF 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.700mg/ml (2.33mM)	Taking the 1 mL working solution as an example, add 50 μL 14 mg/ml clarified DMF stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Working Concentrations for Cell Culture Treatment

Working Concentrations for Animal Model Treatment

Mechanism of Action

Information	Cisplatin is a cytotoxic DNA crosslinker. It affects p53 and DDR signaling by forming DNA adducts, promoting cell apoptosis.
Read more about Cisplatin IC50 for cell-based and cell-free assays
Primary Applications and Mechanism of Action

Chemical Information, Storage & Stability

Molecular Weight	300.05	Formula	Cl₂H₆N₂Pt	Storage (From the date of receipt)	2 years 4°C(in the dark) powder
CAS No.	15663-27-1	Download SDF		Storage of Stock Solutions	Solutions are unstable. Prepare fresh or purchase small, pre-packaged sizes. Repackage upon receipt.
Synonyms	NSC 119875, Cisplatinum, cis-diamminedichloroplatinum II, CDDP, cis DDP, DDP			Smiles	[NH2-].[NH2-].Cl[Pt+2]Cl

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Frequently Asked Questions

Question 1:
What is the appropriate concentration of DMF for cell culture and animal study?

Answer:
It depends on the cell type. The final concentration of DMF should be better limited to less than 0.1% if possible, or below 1%. Using saline as a vehicle for it at up to 3mg/ml is recommended. It's a suspension and can be administrated via oral gavage.

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Cisplatin DNA Synthesis inhibitor

Molecular Weight: 300.05

Quality Control

Solubility in DMSO or Water

Molarity Calculator

In vivo Formulation Calculator (Clear solution)

Working Concentrations for Cell Culture Treatment

Step 1: Select a research area to find working concentrations, or search directly for a cell line

Step 2: Select a cell line to find matched working concentrations

Working Concentrations for Animal Model Treatment

Step 1: Select a research area to find working concentrations, or search directly for an animal type

Step 2: Select an animal type to find matched working concentrations

Mechanism of Action

Chemical Information, Storage & Stability

Tech Support

Frequently Asked Questions