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Flubendazole Autophagy activator

Name: Flubendazole
Brand: Selleck Chemicals
SKU: S1837
Availability: InStock
Rating: 5 (3 reviews)

Cat.No.S1837

Flubendazole (Flumoxanal, NSC 313680) is an autophagy inducer by targeting Atg4B, used to treat internal parasite and worm infection.

Chemical Structure

Molecular Weight: 313.28

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability Specificity

Quality Control

Batch: Purity: 99.89%

99.89

Related Targets	CXCR Nrf2 Mitophagy LRRK2 ULK FKBP Heme Oxygenase LC3 cGAS OGA Beclin
Related Products	Resveratrol Spautin-1 PIK-III DC661 Lys05 Autophinib SMER28 EAD1 EN6 ROC-325 NSC 185058 MPP+ iodide IITZ-01 STF-62247 QX77 CA-5f Lucanthone CA77.1 Daurisoline

Chemical Information, Storage & Stability

Molecular Weight	313.28	Formula	C₁₆H₁₂FN₃O₃	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	31430-15-6	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Flumoxanal, NSC 313680			Smiles	COC(=O)NC1=NC2=C(N1)C=C(C=C2)C(=O)C3=CC=C(C=C3)F

Solubility

In vitro
Batch:

DMSO : 15 mg/mL ( (47.88 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.375mg/ml (1.20mM)	Taking the 1 mL working solution as an example, add 50 μL of 7.5 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.104mg/ml (0.33mM)	Taking the 1 mL working solution as an example, add 50 μL of 2.08 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	Atg4B ^[7]
In vitro	Flubendazole results in morphological changes included contraction of the soma region, formation of blebs on the tegument, rostellar disorganization, loss of hooks and destruction of microtriches in Echinococcus granulosus. ^[1] This compound have a bicyclic ring system in which a benzene has been fused to the -4 and -5 positions of the heterocycle (imidazole). ^[2] It and Albendazole shows similar potency in affecting rat embryonic development in vitro, inducing retardation of growth and dysmorphogenic effects at concentrations ≥0.5 μg/mL. ^[3]
In vivo	Flubendazole (6.32 mg/kg/day) initially induces an arrest of embryonic development followed by a generalized cell death that leads to 100% embryolethality by gestation day (GD) 12.5. This compound (3.46 mg/kg/day) markedly reduces embryonic development by GD 12.5 without causing cell death. ^[4] This chemical in olive oil causes a statistically significant increase in embryolethality at doses of 7.83 mg/kg per day and higher, with complete resorption in all dams at 31.33 mg/kg per day in rats. ^[5] This treatment causes a slight increase of metyrapone and daunorubicin activities in hepatic as well as intestinal cytosol in birds. It also leads to statistically significant inhibition of intestinal GST activity. The treatment leads to slight but significant inhibition (decrease to 69%) of 7-ethoxyresorufin activity in hepatic microsomes. ^[6]
References	https://pubmed.ncbi.nlm.nih.gov/16374619/ https://pubmed.ncbi.nlm.nih.gov/15500567/ https://pubmed.ncbi.nlm.nih.gov/23287076/ https://pubmed.ncbi.nlm.nih.gov/24994687/ https://pubmed.ncbi.nlm.nih.gov/12849847/ https://pubmed.ncbi.nlm.nih.gov/17316720/ https://pubmed.ncbi.nlm.nih.gov/26299935/

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