Spautin-1

Catalog No.S7888

Spautin-1 Chemical Structure

Molecular Weight(MW): 271.26

Spautin-1 is a potent and specific autophagy inhibitor, and inhibits the deubiquitinating activity of USP10 and USP13 with IC50 of ∼0.6-0.7 μM.

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USD 447 In stock
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2 Customer Reviews

  • Autophagosomal inhibition was performed in 32D EV, WT CALR, del52, and ins5 expressing cells. The 32D cell lines were treated with the inhibitor spautin-1 for the indicated time. Lysates were prepared, and SDS-PAGE and Western blotting were performed. Antibodies detecting mutated CALR (CALR mut), CALR, LC3I-II, and GAPDH were used for immunostaining. LC3I-II served as control for successful autophagosomal inhibition.

    J Hematol Oncol, 2016, 9(1):45. Spautin-1 purchased from Selleck.

    (c) PC-9G cells were treated with BIBW2992 (250 nM) or WZ4002 (15 nM) plus SAHA (2 mM) with or without spautin-1 (20 mM) for 24 hr. The cell lysates were harvested and the indicated proteins were analyzed by Western blotting.

    Int J Cancer, 2015, 136(11):2717-29. . Spautin-1 purchased from Selleck.

Purity & Quality Control

Choose Selective Autophagy Inhibitors

Biological Activity

Description Spautin-1 is a potent and specific autophagy inhibitor, and inhibits the deubiquitinating activity of USP10 and USP13 with IC50 of ∼0.6-0.7 μM.
Targets
USP10 [1]
(Cell-free assay)
USP13 [1]
(Cell-free assay)
0.5779 μM 0.6916 μM
In vitro

In Bcap-37 cells, Spautin-1 dramatically enhanced cell death in glucose-free media and induces apoptotic morphology. In Bax-Bak DKO cells, spautin-1 inhibits etoposide induced autophagic cell death. Spautin-1 promots the degradation of Vps34 complexes by regulating the deubiquitination activity of USP10 and USP13, and reduces the levels of PtdIns3P. [1] In PDGF-treated cells, spautin-1 stabilizes α-smooth muscle cell actin and calponin, prevents actin filament disorganization, diminishes production of extracellular matrix, and abrogates VSMC hyperproliferation and migration. [2] In CML cells, spautin-1 markedly inhibits IM-induced autophagy by downregulating Beclin-1, and enhances IM-induced apoptosis by inactivating PI3K/AKT and activating downstream GSK3β. [3] Spautin-1 also specifically reduces infectious dengue virus titers in BHK-21 cells. [4]

Protocol

Solubility (25°C)

In vitro DMSO 54 mg/mL (199.07 mM)
Ethanol 7 mg/mL (25.8 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 271.26
Formula

C15H11F2N3

CAS No. 1262888-28-7
Storage powder
in solvent
Synonyms N/A

Bio Calculators

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Autophagy Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID