EAD1

Catalog No.S8576

EAD1 Chemical Structure

Molecular Weight(MW): 598.45

EAD1 is a potent autophagy inhibitor with an IC50 of 5.8 μM in the BxPC3 cells. It has antiproliferative activities in lung and pancreatic cancer cells.

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Biological Activity

Description EAD1 is a potent autophagy inhibitor with an IC50 of 5.8 μM in the BxPC3 cells. It has antiproliferative activities in lung and pancreatic cancer cells.
Targets
Autophagy [1]
(BxPC3 cells)
5.8 μM
In vitro

EAD1 inhibits autophagy, as judged by the cellular accumulation of the autophagy-related autophagosome proteins LC3-II and p62 and induces apoptosis[1].

In vivo EAD1 is a viable lead compound for evaluation of the antitumor activity of autophagy inhibitors in vivo[1].

Protocol

Cell Research:

[1]

+ Expand
  • Cell lines: H460 cells
  • Concentrations: 0-100 μM
  • Incubation Time: 24 h
  • Method:

    Cell growth inhibition is also assessed in a colony formation assay. H460 cells are plated at 250 cells per well in 24 well plates. After allowing for cell attachment overnight, HCQ and EAD1 are added at the indicated concentrations. After 24 hours, drug-containing medium is removed, and drug-free medium added. Cell colonies are stained with crystal violet after an additional 10-day growth in the absence of drug.


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (167.09 mM)
Water 100 mg/mL (167.09 mM)
Ethanol 100 mg/mL (167.09 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 598.45
Formula

C26H28Cl2F3N7O2

CAS No. 1644388-26-0(free base)
Storage powder
in solvent
Synonyms N/A

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Autophagy Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID