Betulinic acid

Catalog No.S3603

Betulinic acid, a pentacyclic triterpenoid from Syzigium claviflorum, is a inhibitor of HIV-1 with EC50 of 1.4 μ M.

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Betulinic acid Chemical Structure

Betulinic acid Chemical Structure
Molecular Weight: 456.7

Validation & Quality Control

Quality Control & MSDS

Related Compound Libraries

Betulinic acid is available in the following compound libraries:

Product Information

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Product Description

Biological Activity

Description Betulinic acid, a pentacyclic triterpenoid from Syzigium claviflorum, is a inhibitor of HIV-1 with EC50 of 1.4 μ M.
Targets HIV-1 Aminopeptidase N Eukaryotic topoisomerase I
IC50 1.4 μM (EC50) [1] 7.3 μM [2] 5 μM [3]
LD50
In vitro Betulinic acid shows anti-HIV activity by blocking HIV replication in H9 lymphocytes with an EC50 value of 1.4 μM and inhibiting uninfected H9 cell growth with an IC50 value of 13 μM. [1] Betulinic acid also acts as anti-melanoma agent through inhibiting aminopeptidase N activity with IC50 of 7.3 μM. [2] In addition, Betulinic acid inhibits eukaryotic topoisomerase I activity with IC50 of 5 μM and can be exploited as a strong candidate for anti-tumor drug. [3]
In vivo Betulinic acid inhibits prostate cancer cell and tumor (xenograft) growth in athymic nude mice bearing LNCaP cells and this is due, in part, to proteasome-dependent downregulation of Sp1, Sp3, Sp4 and several Sp-regulated genes. [4]
Features

Protocol(Only for Reference)

Kinase Assay: [3]

Topoisomerase I-DNA interaction Annealed 25-mer duplex of oligonucleotide-1 and oligonicleotide-2 is incubated with 25 or 50 units of rat liver topoisomerase I at 8 °C for 15 min in the presence or absence of betulinic acid in binding buffer. After incubation the reaction mixtures are electrophoresed in 7% non-denaturing polyacrylamide gel at 4 °C in 0.167 × TBE buffer and DNA bands are stained with ethidium bromid

Cell Assay: [1]

Cell lines H9 lymphocytes
Concentrations
Incubation Time 3 days
Method H9 lymphocytes ( 1 × 107 cells/ml) are incubated in the presence or absence of HIV-1 (IIIB isolate) for 1 h at 37 °C. Cells are washed thoroughly to remove unadsorbed virions and resuspended in culture medium. Betulinic acid (diluted in test medium) is added to the culture medium. After incubation for 3 days at 37 °C, the cell densities of uninfected cultures are determined to assess toxicity of betulinic acid. A p24 antigen capture assay is used to determine the level of HIV infection in HIV-treated culture

Animal Study: [4]

Animal Models LNCaP tumor xenograft model
Dosages 10 and 20 mg/kg/d
Administration Orally
Solubility 1% DMSO/30% polyethylene glycol/1% Tween 80, pH 4 10 mg/mL
1

References

Chemical Information

Download Betulinic acid SDF
Molecular Weight (MW) 456.7
Formula

C30H48O3

CAS No. 472-15-1
Storage 3 years -20℃Powder
6 months-80℃in DMSO
Syonnyms N/A
Solubility (25°C) * In vitro DMSO mg/mL (<1 mM)
Water mg/mL (<1 mM)
Ethanol mg/mL (<1 mM)
In vivo 1% DMSO/30% polyethylene glycol/1% Tween 80, pH 4 10 mg/mL
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Name Lup-20(29)-en-28-oic acid,3-hydroxy-,(3β)-

Preparing Stock Solutions

Stock Solution (1ml DMSO) 1mM 10mM 20mM 30mM
Mass(mg) 0.4567 4.567 9.134 13.701

Research Area

Tech Support & FAQs

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