Betulinic acid

Catalog No.S3603 Batch:S360302

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Technical Data

Formula

C30H48O3
Molecular Weight 456.7 CAS No. 472-15-1
Solubility (25°C)* In vitro DMSO 47 mg/mL (102.91 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Betulinic acid (ALS-357, Lupatic acid, Betulic acid), a pentacyclic triterpenoid from Syzigium claviflorum, is a inhibitor of HIV-1 with EC50 of 1.4 μ M. It's reported that Betulinic acid acts as a new activator of NF-κB.Phase 1/2.
Targets
HIV-1 [1] Eukaryotic topoisomerase I [3] Aminopeptidase N [2]
1.4 μM(EC50) 5 μM 7.3 μM
In vitro Betulinic acid shows anti-HIV activity by blocking HIV replication in H9 lymphocytes with an EC50 value of 1.4 μM and inhibiting uninfected H9 cell growth with an IC50 value of 13 μM. [1] Betulinic acid also acts as anti-melanoma agent through inhibiting aminopeptidase N activity with IC50 of 7.3 μM. [2] In addition, Betulinic acid inhibits eukaryotic topoisomerase I activity with IC50 of 5 μM and can be exploited as a strong candidate for anti-tumor drug. [3]
In vivo Betulinic acid inhibits prostate cancer cell and tumor (xenograft) growth in athymic nude mice bearing LNCaP cells and this is due, in part, to proteasome-dependent downregulation of Sp1, Sp3, Sp4 and several Sp-regulated genes. [4]

Protocol (from reference)

Kinase Assay:[3]
  • Topoisomerase I-DNA interaction

    Annealed 25-mer duplex of oligonucleotide-1 and oligonicleotide-2 is incubated with 25 or 50 units of rat liver topoisomerase I at 8 °C for 15 min in the presence or absence of betulinic acid in binding buffer. After incubation the reaction mixtures are electrophoresed in 7% non-denaturing polyacrylamide gel at 4 °C in 0.167 × TBE buffer and DNA bands are stained with ethidium bromid
Animal Study:[4]
  • Animal Models

    LNCaP tumor xenograft model

  • Dosages

    10 and 20 mg/kg/d

  • Administration

    Orally

Selleck's Betulinic acid has been cited by 14 publications

Graphene oxide/gallium nanoderivative as a multifunctional modulator of osteoblastogenesis and osteoclastogenesis for the synergistic therapy of implant-related bone infection [ Bioact Mater, 2023, 25:594-614] PubMed: 37056253
N,N-Dimethyl-3β-hydroxycholenamide attenuates neuronal death and retinal inflammation in retinal ischemia/reperfusion injury by inhibiting Ninjurin 1 [ J Neuroinflammation, 2023, 20(1):91] PubMed: 37029422
Embelin attenuates lipopolysaccharide-induced acute kidney injury through the inhibition of M1 macrophage activation and NF-κB signaling in mice [ Heliyon, 2023, 9(3):e14006] PubMed: 36938407
Citrulline inhibits LPS-induced pyroptosis of RAW264.7 macrophages through NF-κB signaling pathway [ Immun Inflamm Dis, 2023, 11(4):e832] PubMed: 37102651
TAZ ameliorates the microglia-mediated inflammatory response via the Nrf2-ROS-NF-κB pathway [ Mol Ther Nucleic Acids, 2022, 28:435-449] PubMed: 35505966
Spironolactone Ameliorates Senescence and Calcification by Modulating Autophagy in Rat Tendon-Derived Stem Cells via the NF-κB/MAPK Pathway [ Oxid Med Cell Longev, 2021, 2021:5519587] PubMed: 34306308
Betulinic acid promotes the osteogenic differentiation of human periodontal ligament stem cells by upregulating EGR1 [ Acta Biochim Biophys Sin (Shanghai), 2021, gmab111] PubMed: 34519779
Long Noncoding RNA MALAT1 Regulates the Progression of Atherosclerosis by miR-330-5p/NF-κB Signal Pathway [ J Cardiovasc Pharmacol, 2021, 78(2):235-246] PubMed: 34554676
11q Deletion or ALK Activity Curbs DLG2 Expression to Maintain an Undifferentiated State in Neuroblastoma [ Cell Rep, 2020, 32(12):108171] PubMed: 32966799
Betulinic acid restores imatinib sensitivity in BCR-ABL1 kinase-independent, imatinib-resistant chronic myeloid leukemia by increasing HDAC3 ubiquitination and degradation [ Ann N Y Acad Sci, 2020, 1467(1):77-93] PubMed: 31930541

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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