Catalog No.S1570

KU-60019 Chemical Structure

Molecular Weight(MW): 547.67

KU-60019 is an improved analogue of KU-55933, with IC50 of 6.3 nM for ATM in cell-free assays, 270- and 1600-fold more selective for ATM than DNA-PK and ATR,and is a highly effective radiosensitizer.

Size Price Stock Quantity  
In DMSO USD 195 In stock
USD 150 In stock
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4 Customer Reviews

  • The ATM-dependent Ser15-p53 phosphorylation was monitored by Western blotting. Protein loading levels were monitored by probing for actin.

    Brain Pathol, 2012, 22(5): 677-88 . KU-60019 purchased from Selleck.

    Relative quantification of stem cell markers (Prom1, Bmi1, CD15, Msi1, Msi2, Nanog, Nestin, Oct4 and Sox2) in control BORRU epidermal growth factor fibroblast growth factor (EGF-FGF) and radiation-survived BORRU after sensitization with KU-60019 [(EGF-FGF) + KU-60019 + 5 Gy]. BORRU under differentiative conditions [fetal calf serum (FCS)] were used as tissue control (DCtref).

    Brain Pathol, 2012, 22(5): 677-88 . KU-60019 purchased from Selleck.

  • (a,b) Drug synergy experiment in AALE cells with ATM inhibitor (KU60019) and trametinib (40 nM). Displayed is relative cell viability for KU60019 treatment alone and for co-treatment with trametinib. Error bars indicate s.d. (n=3). (b) Deviation from Bliss additivity calculated from experiment in a. Error bars indicate s.d. (n=3).

    Nat Commun, 2016, 7:13701. KU-60019 purchased from Selleck.

    Brain Pathol 2012 22(5), 677-88. KU-60019 purchased from Selleck.

Purity & Quality Control

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Biological Activity

Description KU-60019 is an improved analogue of KU-55933, with IC50 of 6.3 nM for ATM in cell-free assays, 270- and 1600-fold more selective for ATM than DNA-PK and ATR,and is a highly effective radiosensitizer.
Features Improved analog of KU-55933, and is more effective at blocking ATM-mediated DDR events.
ATM [1]
(Cell-free assay)
6.3 nM
In vitro

Compared to KU-55933, KU-60019 is an improved more water-soluble inhibitor of the ATM kinase, while displaying similar target selectivity. KU-60019 has little activity against DNA-PKcs and ATR with IC50 values of 1.7 μM and >10 μM, respectively, as well as 229 other protein kinases such as PI3K, mTOR and mTOR/FKBP12. KU-60019 displays 3- to 10-fold more potency than KU-55933 at blocking radiation-induced phosphorylation of key ATM protein targets such as p53, γ-H2AX, and CHK2, in human glioma U87 and U1242 cells, as 1 μM of KU-60019 significantly induces >70% decrease of p53 (S15) phosphorylation to which extent ~10 μM of KU-55933 is required to achieve. KU-60019 effectively radiosensitizes human glioma cells with dose-enhancement ratio of 1.7 and 4.4 at 1 μM and 10 μM, respectively, and also radiosensitizes the normal fibroblasts but not the A-T fibroblasts. KU-60019 treatment (3 μM) blocks basal and insulin-induced AKT S473 phosphorylation by 70% and ~50%, respectively, and completely reduces radiation-induced AKT phosphorylation below the level of control. The effect of KU-60019 on AKT S473 phosphorylation can be seen in glioma cell lines and normal fibroblasts but not in A-T (h-TERT) cells, and can be significantly blocked by phosphatase inhibitor okadaic acid, suggesting a critical role of ATM kinase in regulating AKT phosphorylation via unknown phosphatase. Consistent with the inhibition of prosurvival AKT signaling, KU-60019 at 3 μM significantly inhibits migration and invasion of human glioma U87 cells by >70% and ~60%, respectively, as well as U1242 cells by >50% and ~60% respectively. [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
BAECs NV;2VGdDTnWwY4Tpc44hSXO|YYm= NFi0NJoyOMLizszN MY[xxsBpyqB? M2DjcWROW09? MoPJZYJwdGm|aHXzJJRp\SCyaH;zdIhwenmuYYTpc44hd2ZiQWTNMXNmejF7OEG= MlmxNlU1QTh3NEK=
BAECs M4TxT2Z2dmO2aX;uJGF{e2G7 MWexNOKh|ryP NVPPb|k4OcLiaNMg MUjEUXNQ MlO1bY5pcWKrdIOgeIhmKGmwY4LlZZNmKGmwIF7PV{Bi[3Srdnn0fS=> NFjjVHkzPTR7OEW0Ni=>
U87 MlfYT4lv[XOnIFHzd4F6 NET3TI4{KM7:TdMg MVGwMlUhcA>? MVvEUXNQ MUNCpIlvcGmkaYTzJJRp\SCDVF2gb4lv[XOn MkW4NlM3OjB2MEm=
U1242 M2S2cWFxd3C2b4Ppd{BCe3OjeR?= NHjVWlc{KM7:TdMg MkjtNeKhcMLi MUfEUXNQ NVjJO2hJemGmaX;z[Y5{cXSrenXzJIh2dWGwIHfsbY9u[SClZXzsdy=> NWfTcIdNOjN4MkC0NFk>
U87 NFzrTYhCeG:ydH;zbZMhSXO|YYm= MXWzJO69VcLi M4Lhe|HDqGkEoB?= NXr2WmF6TE2VTx?= M2\sVpJi\Gmxc3Xud4l1cXqnczDoeY1idiCpbHnvcYEh[2WubIO= NXL3W2pMOjN4MkC0NFk>
U1242  MoPXT4lv[XOnIFHzd4F6 Mn7kNE4xOS1|IN88UeKh MXmxJIg> MoHISG1UVw>? MofpZoxw[2u|IFHUUUBscW6jc3WgZYN1cX[rdImgZZQhdG:5IHPvcoNmdnS{YYTpc45{ M4iz[|IzOzdyNEi1

... Click to View More Cell Line Experimental Data

In vivo In orthotopic glioma U1242/luc-GFP xenograft models, the combination of KU-60019 and radiation significantly increases survival of mice than KU-60019 alone, radiation alone, or no treatment. In addition, p53-mutant gliomas is much more sensitive to KU-60019 radiosensitization than wild-type glioma. [2]


Cell Research:[1]
+ Expand
  • Cell lines: U87 and U1242
  • Concentrations: Dissolved in water, final concentrations ~3 μM
  • Incubation Time: 1, 3, and 5 days
  • Method: Cells are exposed to KU-60019 for 1, 3, and 5 days. Cell growth is determined by AlamarBlue. AlamarBlue is added to the medium to the recommended final concentration. Plates are incubated for 1 hour at 37 °C, fluorescence is determined on a Fluoro-Count plate reader (excitation 530 nm, emission 590 nm), and values are taken as a measure of cell growth. Cell survival is determined by trypan blue/fluorescence activated cell sorting (FACS) assay.
    (Only for Reference)
Animal Research:[2]
+ Expand
  • Animal Models: Athymic female mice harboring orthotopic glioma U1242/luc-GFP tumors or human glioma U1242/luc-GFP tumors
  • Formulation: PBS
  • Dosages: KU-60019 (10 μM) is delivered at a rate of 0.5 μL/h by osmotic pump; KU-60019 (250 μM) in 12.5 μL is infused by CED.
  • Administration: Administered intratumorally by convection-enhanced delivery or osmotic pump
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 18 mg/mL warmed (32.86 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 547.67


CAS No. 925701-49-1
Storage powder
Synonyms N/A

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID